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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Senalik D."/>
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                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YJ66"/>
    <dbReference type="Gramene" id="KZM93309">
        <property type="protein sequence ID" value="KZM93309"/>
        <property type="gene ID" value="DCAR_016554"/>
    </dbReference>
    <dbReference type="OMA" id="MADECNA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1290">
        <property type="entry name" value="MYB-RELATED PROTEIN 306-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG99566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="353" mass="39467" checksum="809E63D4B046910E" modified="2016-07-06" version="1">MGRPPCSEKKGVKKGPWTPEEDIILVSYIQEHGPGNWRAVPTNTGLLRCSKSCRLRWTNYLRPGIKRGSFSDQEEKMIIHLQALLGNRWAAIASYLPQRTDNDIKNYWNTHLKKKLSKSEGHGVGHDDNEHDLNGVSSDSSSSGAPKGQWERALQTDIHMAKAALCEALCLDKSPITTTTLASQAPNFTRASPVQSSPTYASSADNIARLLQNWTRKSPNSWSPEITTCHDQYNSNSFNNYHQVGSTSTGSPSSGFGSLYNNFNYSMNNSSSTDVSMDDAANFTTQSKHDKGKLMLQDYDVTKPIVKNNSSHYDHQQVHEHEELPPLTLLEKWLFDDVTMAAPPQDDLMMNMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZXI5</accession>
    <name>A0A161ZXI5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_020805</name>
        <name evidence="13" type="ORF">DCAR_0625540</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Intracellular vesicle trafficking and protein transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZXI5"/>
    <dbReference type="STRING" id="79200.A0A161ZXI5"/>
    <dbReference type="Gramene" id="KZM91830">
        <property type="protein sequence ID" value="KZM91830"/>
        <property type="gene ID" value="DCAR_020805"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224745"/>
    <dbReference type="OMA" id="YSTNRDC"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000456">
        <property type="entry name" value="Ras-related protein RABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM91830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH06117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="213" mass="23471" checksum="23D1663E4220383D" modified="2016-07-06" version="1">MGSSSGQGGGSNYDLSFKILLIGDSAVGKSSLLLTFISNSVDQDISPTIGVDFKIKQLMVGGKKLKLTIWDTAGQERFRTLTSSYYRGAQGIILVYDVTRRDTFTNLSDVWAKEVDLYSTNQNCVKMLVGNKVDIESERVVSREEGIALAKEHGCLFLECSARTRENVEQCFEELALKIMEVPSLSEEGSSVVKRNILKQKPEYQTQSGGCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AN07</accession>
    <name>A0A162AN07_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_012209</name>
        <name evidence="7" type="ORF">DCAR_0313831</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG94535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN03453">
        <property type="protein sequence ID" value="KZN03453"/>
        <property type="gene ID" value="DCAR_012209"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211806"/>
    <dbReference type="OMA" id="NVYEHEP"/>
    <dbReference type="OrthoDB" id="622307at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF220">
        <property type="entry name" value="LOW QUALITY PROTEIN: NAC DOMAIN-CONTAINING PROTEIN 90-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="4"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZN03453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG94535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="27531" checksum="F50AC2D468C59E7B" modified="2016-07-06" version="1">MATFQPGFRFYPTEEELVSFYLSHRLQGHNQTDLDRVIPVVDIYDLEPSQLPNYSGELCREDKEQWFFFVPMHEREARGGRTNRTTASGYWKATGSPSYVYSSQNKVIGVKKTMVYYRGKAPLGRKTRWKMNEYKAIEEQVIAGASTSCAAPRLRHEMSVCRVYVISGTFRAFDRRPLGTAIMEPVDEPQDGQENIVTRTSSSAEYIYRETDIGNTMIIDNAGVSCSEPMIIHHAAPFSGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B4U3</accession>
    <name>A0A162B4U3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_002679</name>
        <name evidence="3" type="ORF">DCAR_0102806</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B4U3"/>
    <dbReference type="Gramene" id="KZN10023">
        <property type="protein sequence ID" value="KZN10023"/>
        <property type="gene ID" value="DCAR_002679"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN10023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="261" mass="28913" checksum="27E878D04B1D736F" modified="2016-07-06" version="1">MVMGKKNESEKEEESNASPGEERNRTRGEAEIKASQEPKIEEEESSDDEGPFPEPYPVKRIPVKYYCCSSDSLDSSDSDSEESNASPGEDRNRTRGEAEIKASQEPKIEEEESSDDEGPFPEPYPVKRIPVKYYCCSSDSLDSSDSDSEESNDSPGEDRNRTRGEAEIKAGEYPPLDYSQEAKEPSPGRMVPRNVPHSDSSDFSDFESKNVPETQANKSVLANDLMSASSILVLADPSLFLFFSCVFVSKNLHLVSSSTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B897</accession>
    <name>A0A162B897_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic</fullName>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Beta-ketoacyl-ACP synthase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_003929</name>
        <name evidence="22" type="ORF">DCAR_0104178</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B897"/>
    <dbReference type="STRING" id="79200.A0A162B897"/>
    <dbReference type="Gramene" id="KZN11273">
        <property type="protein sequence ID" value="KZN11273"/>
        <property type="gene ID" value="DCAR_003929"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202936"/>
    <dbReference type="OMA" id="TACSIGN"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000027">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004970">
        <property type="entry name" value="PRK06333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="20">
        <location>
            <begin position="56"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KZN11273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG84992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="469" mass="49743" checksum="ABBEB0C664A66E1C" modified="2016-07-06" version="1">MQALQSSPLRVSPLDPLRKPTSGAATVTNVRPPHKKTFISAAATTTSAPKRETDPSKRVVITGMGLVSVFGNDVDTYYDKLLAGESGIGLIDRFDASKFPTRFGGQIRGFSSEGYIDGKNDRRLDDCLRYCIVAGKKALENADLGGDKRFKVDKERAGVLVGTGMGGLTVFSDGVQALIERGHRKITPFFIPYAITNMGSALLAIDLGFMGPNYSISTACATSNYCFYAAANHIRRGEADLMIAGGTEAAIIPIGLGGFVACRALSQRNDDPKTASRPWDKDRDGFVMGEGAGVLVMESLEHAMKRGAPIIAEYLGGAINCDAYHMTDPRSDGLGVSSCIQSSLIDAGVSPEEVNYINAHATSTLVGDLAEVNAVKKVFKNTSGIKMNATKSMIGHCLGAAGGLEAIAVIKAITTGWLHPTINQFNSEPSVEFDTVANQKQQHEVNVGISNSFGFGGHNSVVAFSAFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TH21</accession>
    <name>A0A164TH21_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_024648</name>
        <name evidence="3" type="ORF">DCAR_0728325</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TH21"/>
    <dbReference type="STRING" id="79200.A0A164TH21"/>
    <dbReference type="Gramene" id="KZM87514">
        <property type="protein sequence ID" value="KZM87514"/>
        <property type="gene ID" value="DCAR_024648"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194729"/>
    <dbReference type="OMA" id="EAECEYG"/>
    <dbReference type="OrthoDB" id="660486at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF118">
        <property type="entry name" value="OS01G0924966 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM87514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="17552" checksum="C6E7D759D466550B" modified="2016-07-06" version="1">MDLVKAKEKKGLIIKTWERCRSFGGRKNYSSQIRRPLVKKSKSCPQLEYTPANKYNCGQKRVSVPEGCFSVYVGPEKQRFVIKTRYVNHPLFKTLLEEAESEYGYCSDGPLALPCNVDIFGKLLLEMDSDEIHQKGCHFTRSVSSYRVLSHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UDT1</accession>
    <name>A0A164UDT1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_025833</name>
        <name evidence="2" type="ORF">DCAR_0729684</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UDT1"/>
    <dbReference type="STRING" id="79200.A0A164UDT1"/>
    <dbReference type="Gramene" id="KZM88758">
        <property type="protein sequence ID" value="KZM88758"/>
        <property type="gene ID" value="DCAR_025833"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008:SF0">
        <property type="entry name" value="CSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM88758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH10219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="150" mass="16786" checksum="52FF1D00063F3268" modified="2016-07-06" version="1">MLTLWWIRFVKFVGTPEVCFTSLHLLLKPGLLHILASASETCWSSNEALSSISYATFADIRIRSAMPNLSSLRVLATICLADDRDHLKHVLQSQKSLLQREEAMAYARTLVAGFEMEHIDELIFFADAFGTSCLSLDYKITPRSGKSACI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UM07</accession>
    <name>A0A164UM07_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_026141</name>
        <name evidence="12" type="ORF">DCAR_0730019</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UM07"/>
    <dbReference type="Gramene" id="KZM89066">
        <property type="protein sequence ID" value="KZM89066"/>
        <property type="gene ID" value="DCAR_026141"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196254"/>
    <dbReference type="OMA" id="TVIKEMM"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
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                <person name="Zeng P."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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            <dbReference type="ARBA" id="ARBA00005536"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
    <sequence length="538" mass="59340" checksum="287D5E39EB5A98D7" modified="2016-07-06" version="1">MSMLDSFFNKGFKAPRCKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREEKMMAAQEIIELFCELISVRLPIIESQRECPLDLKEAISSICFAAPRCADLPELIQVQMLFAGKYGKEFVVAATELMPECGVNRQLIELLSVRAPAPDVKLKLLKDIAEEHELDWDPTASESELLKPHEDLLNGPTQFVSETKVPLPEEKKDDDVDDTGSEQVPHYASDSDAGFDTSDFPEVPTASLRPSKGTGTAPEMLPFPASALSGLDPESTNSSGDEESYSHDLKAELEEMLRDKPESEDFKPPSTLVDEDKQFLPFISPPPPSSVSLPPPSSSTALEREINPPPSVLRTSTDDVDLLDVLAAAQAAASSAEHAAAAARSAASLAEFRINEIVSKRNVGGSQITGDNPFHSGSSVSDTGKKQALDHENSFTDSDISNSLHSRLHLGDHPELETTKYPEYDNSELNSNPSISENLNLGQYTPPHQPKRVDSLEDESYLSYPNLFNRGPNSVSREPSFDDKFDSAGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XEF5</accession>
    <name>A0A164XEF5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0518660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XEF5"/>
    <dbReference type="Gramene" id="KZM93065">
        <property type="protein sequence ID" value="KZM93065"/>
        <property type="gene ID" value="DCAR_016310"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220879"/>
    <dbReference type="OMA" id="MYKSYTP"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050266">
        <property type="term" value="F:rosmarinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000015">
        <property type="entry name" value="Spermidine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF11">
        <property type="entry name" value="SHIKIMATE O-HYDROXYCINNAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="432" mass="47537" checksum="27F67E940DF0BCF5" modified="2016-07-06" version="1">MKITVKESTLVPPAVETPRRRLWNANVDLVVPNFHTPSVYFYRPNGGDNFFDTKVLKDALSRALVPFYPMAGRLKRDDDGRVEIDCNGEGVLFVEAESDGVVDDFGDFAPTLELRQLIPAVDYSLGISSYSLLVLQITFFKCGGVSLGVGMQHHAADGASGLHFINTWSDMARGLDLTLAPFIDRTLLRAREPPQPAFPHIEYQAPPSMKLNPDPKESTPETSVSIFKLTRAQLNALKAKAKEDGNTVAYSSYEMLAGHVWRSVCKARGLADDQESKLYIATDGRFRLLPPLPAGYFGNVIFTATPIAAAGDLMSKPLWFGASRIHNALARMDNDYLRSALDYLELQPDLKALVRGAHTFKCPNLGITSWGRLPIHDADFGWGRPIFMGPGGIAYEGLSFVLPSPCNDGSLSVAISLQAEHMKLFSKLLYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XMM7</accession>
    <name>A0A164XMM7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_016611</name>
        <name evidence="4" type="ORF">DCAR_0518986</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XMM7"/>
    <dbReference type="STRING" id="79200.A0A164XMM7"/>
    <dbReference type="Gramene" id="KZM93366">
        <property type="protein sequence ID" value="KZM93366"/>
        <property type="gene ID" value="DCAR_016611"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220779"/>
    <dbReference type="OrthoDB" id="1925898at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36347">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36347:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM93366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18054" checksum="57539BBBFD9D9FE1" modified="2016-07-06" version="1">MNTTLFSFTAKPLTTTPSKFQLHHQKYLLSVTPLKSSTDSTNSKITSKDGEVGAPSSPEVAPIRFKRVSRRRARQQELEQSKPAPKQPKPPKEWEAMTLTEKALELYVGEKGLLFWINKFAYASIYIIIGAWICFRFVGPALNLYQLDAPPLSPTDVLKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A265</accession>
    <name>A0A165A265_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0414853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A265"/>
    <dbReference type="Gramene" id="KZM96789">
        <property type="protein sequence ID" value="KZM96789"/>
        <property type="gene ID" value="DCAR_015849"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="79" mass="9227" checksum="14143B5D34B9234F" modified="2016-07-06" version="1">MFRVALFYGLGNQSTEPMVNIDGNYYVLILPFSEEVGWMSDEKKHRRAEDEERRLTACLDEVRCTICNLRRGCAINSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A9U9</accession>
    <name>A0A165A9U9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WFD4"/>
    <dbReference type="Gramene" id="KZM97407">
        <property type="protein sequence ID" value="KZM97407"/>
        <property type="gene ID" value="DCAR_015231"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218605"/>
    <dbReference type="OMA" id="PRATGHW"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000027">
        <property type="entry name" value="Splicing factor 3B subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000041">
        <property type="entry name" value="Splicing factor 3b subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="14"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="6">
        <location>
            <begin position="442"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="858"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="815"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="830"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG96227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1211" mass="134624" checksum="062AF42A59AA4697" modified="2016-07-06" version="1">MYLYNLTLQQATGIVCAINGSFTGGKSQEIVVARGKVLDLLRPDDNGKIQTILSVEIFGAIRSLAQFRLTGAHKDYIVVGSDSGRIVILEYNKEKNVFDKIHQETFGKSGCRRIVPGQYLGIDPKGRAVMVGACEKQKLVYVLNRDTSARLTISSPLEAHKSHTIVYSIVGVDCGFDNPIFAAIELDYSEADQDSTGQAASEAQKHLTFYELDLGLNHVSRKWSEQVDNGANMLVTVPGGGDGPSGVLVCAENFVIYKNQGHPDVRAVIPRRADLPAERGVLIVSAAMHKQKSMFFFLLQTEYGDVFKVTLDHDNERVSELKIKYFDTIPVTSSLCVLKLGFLFAASEFGNHGLYQFQAIGDDPDVESSSATLMETEEGFQPVFFQPRGLKNLVRIDQVESLMPIMDMKVTNLFEEETPQIFSLCGRGPRSSLRILRPGLAISEMAVSQLPGVPSAVWTVKKNVNDEFDAYIVVSFTNATLVLSIGETVEEVSDSGFLDTTPSLDVSLIGDDSLMQVHPTGIRHIREDGRINEWRTPGKRTIVKVGSNRLQVVVALSGGELIYFEVDMTGQLMEVEKHEMSGDIACLDIAPVPEGRQRSRFLAVGSYDNTIRILSLDPDDCMQVLSLQSVSSPPDSLLFLEVQASIGGEDGADHPASLFLNAGLQSGVLFRTVVDMVTGQLSDARSRFLGLRAPKLFSVAVRGRRAMLCLSSRPWLGYIHQGHFLLTPLSYETLEYAASFSSDQCAEGVVAVAGDALRVFTIERLGETFNETTIPLRYTPRKFVIQNKRKLLVIIESDQGAFAAEDREAARKECFEAAGMGENGKTETENGADDEDKDDPLSDEQYGYPKAESDKWVSCIRVLDPRTTETTCLLELQDNEAAFSICTVNFHDKEYGTLLAVGTAKSLQFWPKRSFSAGYIHIYRFLKDGKSLELLHKTQVDGVPLALSQFQGRLLAGIGPVLRLYDLGKRRLLRKCENKLFPNTINSIHTYRDRIYVGDIQESFHYCKYRRDENQLYIFADDSVPRWLTASYHIDFDTMAGGDKFGNIYFVRLPQDVSDEIEEDPTGGKIKWEQGKLNGAPNKVEEIVQFHVGDVVTCLQKASLIPGGQESLIYGTVMGSLGALLAFSSRDDVDFFNHLEMHMRQEHPPLCGRDHMAYRSAYFPVKDVVDGDLCEQFPTLPMDLQRKIADELDRTPGEILKKLEEIRNKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WTU6</accession>
    <name>A0A165WTU6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">60S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_014982</name>
        <name evidence="9" type="ORF">DCAR_0415842</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WTU6"/>
    <dbReference type="STRING" id="79200.A0A165WTU6"/>
    <dbReference type="Gramene" id="KZM97656">
        <property type="protein sequence ID" value="KZM97656"/>
        <property type="gene ID" value="DCAR_014982"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216105"/>
    <dbReference type="OMA" id="CTIDNCH"/>
    <dbReference type="OrthoDB" id="10250051at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="75"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM97656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG96506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17898" checksum="1521A707FF36E30C" modified="2016-07-06" version="1">MPPKFDPSQVIDVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVSVVPSAAALVIKALKEPERDRKKTKNIKHTGNISLDDVIEIAKIMRPRSMAKELTGTVKEILGTCVSVGCTVDGKDPKDLQEEIDDGEVEIPMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WTV6</accession>
    <name>A0A165WTV6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_014980</name>
        <name evidence="2" type="ORF">DCAR_0415844</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WTV6"/>
    <dbReference type="Gramene" id="KZM97658">
        <property type="protein sequence ID" value="KZM97658"/>
        <property type="gene ID" value="DCAR_014980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM97658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG96508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="6531" checksum="DBE52E615C8B6BB8" modified="2016-07-06" version="1">MHAWLPACHLQNPSSEVDGVWRMACEEPCMPLFYFLLDSAHCSVNVCYSWLMFLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XS50</accession>
    <name>A0A165XS50_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0416762</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XS50"/>
    <dbReference type="Gramene" id="KZM98459">
        <property type="protein sequence ID" value="KZM98459"/>
        <property type="gene ID" value="DCAR_014179"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215929"/>
    <dbReference type="OMA" id="LWAYRAP"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000088">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045:SF20">
        <property type="entry name" value="UDP-RHAMNOSE:RHAMNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG97422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="466" mass="52648" checksum="6669E7C045C796F8" modified="2016-07-06" version="1">MASGEKLHIVMFPWLAFGHLLPYLKLAKLIAEKGHKISFISTPRNIHRLPKLPHDIAPLINLVKIPLPCTPNLPENAEATCDVPFNKVKHLKIAYDQLQDPVTCFLESNSVDWILCDFASYWLGPVASRLGVPCAFYSIFPASILGFFGPPSQMITGDDYRVIPEDFTVKPKWVPFQTSVAMSLYQILVLEPNFEADETENVSDLFRIGNTIQNCDIVVIRSSTEFEPEWLKLVEEIYKKPVIPAGLLPAIDDDHEDEYWDEIKDWLNKQARGSVIYVAFGAETKLNQVQVTELALGLELSGLPFLWALRKQRGLTDPERIELPEGFEERTRGRGMVYPTWVPQVKILSHDSVGGLLFHSGWSSVVEAVQFGKALVLLPFLGDQGLIAKQVEEKKLGLLIPRNETDGRFTRDSVAETIKLVLVDAEGKIYRDKVKEMKGIIGDMDRQNYYVENLIGYLQNHKSMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XVU3</accession>
    <name>A0A165XVU3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0416893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XVU3"/>
    <dbReference type="Gramene" id="KZM98581">
        <property type="protein sequence ID" value="KZM98581"/>
        <property type="gene ID" value="DCAR_014057"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217383"/>
    <dbReference type="OMA" id="IWKCRTA"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF66">
        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="459" mass="51687" checksum="8B1FC4BDBC4CB3C5" modified="2016-07-06" version="1">MDTKLSFRVKRKPAELITPAKPTPNDQKPLSDLDDREGLRYKLSGVQIYAKSPCMENDDPVKAMREALAKTLVFYYPFAGRLREGPDRKLTVDCSGQGVVFVAAEADVRAEEFPLYPPFPCMDQLLYDHQHGSQEIVNSPLMFIQVTRLLRGGFIFAIKFTHNICDAFGMSQFLTALGEIARGRTSPSVFPIWKRELLNVRNPPQPTCTHHEFTHLTTRVNVTTNHSPDSEMVYRSFFFNSTNISVLRNHVPPHLAKCSTFDLLAACIWRNRTIALALHPEQKVRLVFAVNARSRLKSCIPVGYYGNVFVLPGVISTVEELCNKPIGYALELVMNAKANVTEEYVRSFIDLTVMKGRAPISTEWTYIVDDLTQLGLWDTDYGWGKPVYGGMAYSEVDPVPGTGNYFLRIRKKDGNEDEIVAPMCLPACAMQRFDKELKKLLVYGNSGSQIVCIKNLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AXE0</accession>
    <name>A0A166AXE0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AXE0"/>
    <dbReference type="Gramene" id="KZN02060">
        <property type="protein sequence ID" value="KZN02060"/>
        <property type="gene ID" value="DCAR_010814"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG93007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="20422" checksum="23E1D8AEB4CB2FDA" modified="2016-07-06" version="1">MDPRGTKRKHSESVQDGLVVELTKLNNDSANLNSDMKAANEDFAMMCKSMTDRQAEILTRGLFLEEQHRRVIELLNGGEDVVGVTSTGDEERARTSGNTDIRKLARESDESLRDHRVYREIELQEQEKLFKQYKRMLEQNMIEMRERAEIIEKQRRLLSPKLIQLSRSEAEGSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DIJ1</accession>
    <name>A0A166DIJ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_006141</name>
        <name evidence="4" type="ORF">DCAR_0206921</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DIJ1"/>
    <dbReference type="Gramene" id="KZN05304">
        <property type="protein sequence ID" value="KZN05304"/>
        <property type="gene ID" value="DCAR_006141"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206988"/>
    <dbReference type="OMA" id="ILCNQKS"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF67">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872200" description="GDSL esterase/lipase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN05304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG87690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="38689" checksum="F5A9019ACDBEB6D0" modified="2016-07-06" version="1">MQKYADILKQISCLIFLLSALGDLHVSHGQALVPALFIFGDSVVDVGNNNEIYTIIKSNFPPYGRDFISHMPTGRFCNGKLATDFTAENLGFTTYPPAYLSKQARGKNLLLGANFASGSSGYYEDTAKIYHTIPLSKQVLYYKDYQTKLISIAGISNASSIISGSIHLLSSGASDFVQNYYVNPTLYKNYTPDQFSDILMQYYATVVQQLYTLGARKIGVTTLPPLGCLPASITLFGDGSNQCLPNMNAVALSFNNKLNATSLRLQSKLTGLNLVVLDIYQSLYDLVTKPANFGFSEARRACCGTGLLETSILCNAKSVGTCANASQYVFWDGFHPSEAANKILADNLLASGIALVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166E552</accession>
    <name>A0A166E552_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL5c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">50S ribosomal protein L5, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207842</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166E552"/>
    <dbReference type="Gramene" id="KZN06107">
        <property type="protein sequence ID" value="KZN06107"/>
        <property type="gene ID" value="DCAR_006944"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205886"/>
    <dbReference type="OMA" id="KLKAHHF"/>
    <dbReference type="OrthoDB" id="539541at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_uL5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_uL5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000585">
        <property type="entry name" value="PRK00010.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="260" mass="28598" checksum="679431C6AB82F096" modified="2016-07-06" version="1">MASPALLHSSASSFHSHFPALSSRGSLRLPGRHSLTVKAETNIVLVEKSQAETTSRLKKSYLETIVPLLKDEFSYTNIHQVPKVTKIVVNCGIGDAAQNSKGLDAAVNELALITGQRPVKTRAKNAIATFKIREDQPLGIAVTLRGNVMYSFLDRLINLGLPRTRDFQGVSPNSFDGNGNYSIGFREQSVFPEISYDALGKPRGMDVCISTTAPTDKEAHKLLALMGMPFREGAVSSNVVRKKKLKRHHFDSKAKQRARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F8H0</accession>
    <name>A0A166F8H0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_008309</name>
        <name evidence="7" type="ORF">DCAR_0209393</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F8H0"/>
    <dbReference type="STRING" id="79200.A0A166F8H0"/>
    <dbReference type="Gramene" id="KZN07472">
        <property type="protein sequence ID" value="KZN07472"/>
        <property type="gene ID" value="DCAR_008309"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206171"/>
    <dbReference type="OMA" id="LRFDNQQ"/>
    <dbReference type="OrthoDB" id="1916924at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044533">
        <property type="entry name" value="FLZ1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057:SF9">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="5">
        <location>
            <begin position="88"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="88"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZN07472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="168" mass="19121" checksum="4F11E2A98399BB79" modified="2016-07-06" version="1">MDSTITPRRPCFIEEDDGLASLADMEAGFSGSNNQNNAQINHNQHNHFISRPVYYSRKASLRKMSFSSNTSPRFGRLYDARFEEQQPHFLDSCFLCKKPLGDNKDIFMYRGDTPFCSEECRQEQIEADEAKEKNWNLKALRNKSDQKKSTSSASKKAYGFRTGAVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FTN7</accession>
    <name>A0A166FTN7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FTN7"/>
    <dbReference type="Gramene" id="KZN08162">
        <property type="protein sequence ID" value="KZN08162"/>
        <property type="gene ID" value="DCAR_001227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="284" mass="31852" checksum="111626C81957A6FF" modified="2016-07-06" version="1">MEDLKLLSHNLENALWKWWLTLFLFLSSYKIYSGFEEKIDIELEAEKFPDWIIQSSVECSADDEISDGDNKFSLDLEVIDAATPPPTLKRKRFDPVEVQTSLANSVMPPMSQAAPAFVPRRDFPKHLDPNRAGSSAVNPDGPVQVHDKGKAKLFPEFPVDNENAIFKSGFNFLESDVNLSSADFFEEEQGENMAFGNDEMFTKDVFQLNVDGDTQIFASFLNETHDLGFLTTTQAPGSFLEVDQVLSDMGFTEPTNMAVENATGKKEENEADVRTSKIRRSSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WPC4</accession>
    <name>A0A169WPC4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_005737</name>
        <name evidence="9" type="ORF">DCAR_0206454</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WPC4"/>
    <dbReference type="Gramene" id="KZN04900">
        <property type="protein sequence ID" value="KZN04900"/>
        <property type="gene ID" value="DCAR_005737"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205838"/>
    <dbReference type="OMA" id="AYFHYFR"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="UniPathway" id="UPA00213"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016135">
        <property type="term" value="P:saponin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000167">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF870">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 75B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN04900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="51473" checksum="FCF609CDAE6FC2B6" modified="2016-07-06" version="1">MAHQRHILLITFPAQGHINPSLQFAKKLTSMGAEVTFATSLSAHTRMAKTLAATKGLHLASFSDGYDDGFKLTDDANKFMSSIRSHGSERVREILRSSAEKGRPVSCVVYTLLLPWVAEVAREIHVPSALLWIQPAAVLDLYYYYFNGYGDAMKNCLDDPEWSIQLPGLPRLHARDLPSFLLPNCHEMYSFALPSFKEQLDVLNADEEPKVLVNSFDALECEALRAVDKLKLIAVGPILPSAFLDGKDPSDTSVGGDLFQKSRNYIDWLDSKARRSVVYISFGSILTLSKHQVEEIGRGLLKSGMPFLWVIRKKEGENGLEEEKLSCMEELEQQGMIVPWCSQLEVLSHPSLGCFVTHCGWNSTLESLVSGVPVVAFPHWTDQTTNAKLIEDVWKTGVRVNGDEKGVVDGDEVHRCLELVMGDEVLRRNAEKWGDLAREATKGGSSDKNLKAFVEDFDQQVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WPZ9</accession>
    <name>A0A169WPZ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CP12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_005755</name>
        <name evidence="5" type="ORF">DCAR_0206478</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WPZ9"/>
    <dbReference type="STRING" id="79200.A0A169WPZ9"/>
    <dbReference type="Gramene" id="KZN04918">
        <property type="protein sequence ID" value="KZN04918"/>
        <property type="gene ID" value="DCAR_005755"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206840"/>
    <dbReference type="OMA" id="PLENYCQ"/>
    <dbReference type="OrthoDB" id="4362at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080153">
        <property type="term" value="P:negative regulation of reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039314">
        <property type="entry name" value="CP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003823">
        <property type="entry name" value="CP12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33921">
        <property type="entry name" value="CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33921:SF15">
        <property type="entry name" value="CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02672">
        <property type="entry name" value="CP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01093">
        <property type="entry name" value="CP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP12" evidence="3">
        <location>
            <begin position="59"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR639314-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN04918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="13800" checksum="7CE5747EC96EABB6" modified="2016-07-06" version="1">MAATLSGISLSTPGFLAKAIEAPTVKSPWLKLSQPWKKSTQFSAGRMILRPVAAKPADLSDKVAESIKEAEEACAGDPVSGECVAAWDEVEELSAAASHAKDKKKDSDPLEEYCKENPETQECRTYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE12</accession>
    <name>A0A175YE12_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA N-glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">rRNA N-glycosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0933403</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.22" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YE12"/>
    <dbReference type="Gramene" id="KZM81451">
        <property type="protein sequence ID" value="KZM81451"/>
        <property type="gene ID" value="DCAR_029064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453:SF9">
        <property type="entry name" value="ALBUMIN B-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7 9" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0800">Toxin</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH13890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="410" mass="47877" checksum="6D9D077B9BDD9989" modified="2016-09-07" version="1">MTSTSQPTAVSSDEPLTLNLDKNEKDEYEKFIKSLIKEFPPCDVEKELRNFDKESADKELKSKIEKCFPGIPGNAASTRQKESVRRTCSIEELKDKNWLLKVRLKHECRNLDILIKKNNLYLVGYKGKYKEKEEDGAVNAETEQADYWIVLTGKNHREVVKSFQKFPPSKADRSAEKEEKSKMVLARKGRDKERKITDAVTELKAMKEQIERLKGKDNKDLIKRVDTLLAMVPRDITLERVKELIGADDKAALDFLNYLKEPREDYNPERQHRKFIEQSVKVDNEIRRVKDRLAKVKLNRRAFIKAVNYLTASSLDQEDRKFAKHIIKLALMICEAVRFQSIAEHVACNYYHKETHTDSNLPENCITSIYYWGDRSALMQRGGWYDAERRLLGENPTVIRQMDKDKRDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFE9</accession>
    <name>A0A175YFE9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0933999</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFE9"/>
    <dbReference type="Gramene" id="KZM81901">
        <property type="protein sequence ID" value="KZM81901"/>
        <property type="gene ID" value="DCAR_029514"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201199"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF316">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="224"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="69"/>
        </location>
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        <location>
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            <end position="264"/>
        </location>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="289" mass="31484" checksum="5350CD0E238B4157" modified="2016-09-07" version="1">MANPSSEMEQNLRCPRCDSEETKFAYFNNNKTSQPRYRCKSCKKLWTHGGKVRDISSNGEERRVNRSRDSSPAIVSSAPPPPPLPSSMTSAGGIINETGRLNLTRPPPNHNQPPLKFVRMDIQNLSIKPLEPPHDVGQNNIVQQNQQVLIENYSQGFHAQQTPSNPGNQSFLNTTHSSNYIEPNINTFGNNPIGGFTGGPSGSWGWSSAPVHLYIEPTNAHTFGENNNGMGGPNSNPSGMSSSAWSSSPPSYYIEPNNAWNNNNPMDGSARDPSGSSSGAWSSRAWSSN</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="170" mass="18595" checksum="113180755A8A91BA" modified="2024-05-29" version="1">MAQVKRDDIVSLWTQGVQAVKDKSSCAAVITAKELSIVWGSDSRYWKWALEVAELIEVCWLEIDGKYSAINLIKGVNYGVYFVVELSDNLCMNGPVTLKLTCPNGTTEEHKEYLETMPKNTLVGLRVGEFCDTAACGCENTVKFSMNGCDGTTWKTGLTVIGAVIAPVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9V3</accession>
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        </recommendedName>
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    <gene>
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    <organism evidence="12 13">
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG86002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108204914"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000143">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF254">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041769532" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG86002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="493" mass="55068" checksum="A7137CF59656981E" modified="2024-05-29" version="1">MTVNTFLSIFIFCILLCFGLIQSAPETALVTHLPGFNGAFPSKHYSGYVPIGAADGKKLFYYFVLSERNPTEDPVVLWLNGGPGCSSFDGFVYEHGPFNFEKGDSQNSLPKLHLNPYSWSKVSNIIYLDSPAGVGFSYSGNESDYITGDQKTAIDSHKFLLKWFELYPEFLDNPFYIVGESYAGIYVPTLSFEVVKGIDANVKPAVNFKGYMVGNGCTDDKFDGNALVPFAHGMGLISDELYQEVITECQGNFYNPANDNCESKLGKVDREISGLNIYDILEPCYHGSENKITTVTTRLPQSFRKLGETERPLPVRTRMFGRAWPLRATVRDGIVPTWPQLLNSGNVPCTDDEVATSWLNDETVRKAIHAEQASVAGEWVLCTGRISFRHDTGSMIKYHRNLTSRGIRALIFSGDHDMCVPYTGSEAWTRSMGYEIVDEWRPWIVKEQVAGYIQGYGNNLTFLTVKGSGHTVPEYKPAEALAFYSRWLAGEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WB44</accession>
    <name>A0AAF0WB44_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0205892</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WB44"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF28">
        <property type="entry name" value="XYLOGLUCAN O-ACETYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="48"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="103"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG86675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="46413" checksum="1E258BCC6713C9F4" modified="2024-05-29" version="1">MKNYFSLYCYVCIFLCISYFLIHSYPLEIFNKVTPQENHVSNLIQRYQKCDLFTGHWIRDMEQPLYTSKSCPIMGDLRNCFLNGRVDTDFLKWRWQPDTCEISRFDPKLFLEMVRGKSMAFIGDSVARNHMESLLCLLYQGEIPENIYWAEEGRILKYHFRNHDFTLMVLWTPFLVEENEIVSHGIKTDNYTLHLDKVHEVWLKQLPSSLDYAIISTGHWFFNRRFYLYQDGALIGCVSCSEPDIPKYDFKFAVRMSIRTALEYTERFKGITIALRTFSPGHFENGGWNAGGSCTRTSPLHELSDAYKIISTYNVTLELRGVQLEELERAKRRGGADRRLLPLDVTRAMLMRPDGHPDIHFGNVWKRGSHDCVHWCLPGPIDAWNDLLFAALLKETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBP1</accession>
    <name>A0AAF0WBP1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0205743</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBP1"/>
    <dbReference type="KEGG" id="dcr:108203234"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF9">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Rolling W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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    </reference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
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    </dbReference>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="8">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="211"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
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        <location>
            <begin position="350"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="402" mass="45107" checksum="FB7D58B3DDA0607B" modified="2024-05-29" version="1">MDRDFMAAPAAASPPIVSPAPVNKSLAPGFRFHPTDEELVRYYLRRKACGKPFRFQAVTEIDVYKSEPWELAGHSSLNTRDMEWYFFSPVDRKYGNGSRLNRATGKGYWKATGKDRCVRHKGETIGMKKTLVFHSGRAPDGQRTNWVMHEYRLVDIELQQAGVAQDAFVLCRIFQKSGLGPPNGDRYAPFIEEEWEDDAALFVPGGEAEEDVANASEGRTGVNDIEQIPDEDKAPLPSESLIDHQTIPFVCKRERTEDQPMNDEADPEPLSMLFEPKRAKQSDPNSSHQNGSEDSTTSQEPTTAPMSLALMVFPLLENVGTETSTQQVNPPTFDADNLEKSVPPGYLKFISNLENEILSVSMERETLKIEVMRAQAMINILQSHIEVLTKDNEDLKRAAQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WH68</accession>
    <name>A0AAF0WH68_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108209493"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Double-stranded RNA-binding protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044451">
        <property type="entry name" value="AtDRB-like_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031:SF33">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
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        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG88030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="513" mass="55729" checksum="1A5C8645CACD4B94" modified="2024-05-29" version="1">MYKNQLQELAQRSCFNLPAYTCIREGPDHAPRFKATVNFNGETYECPNYCTTLRQAEHSAAEVALNALANRGPSNSLAARILDETGVYKNLLQEVSQRVGASLPTYKTSRSGLGHLPIFTCTVELAGVTFTGEPAKNKKQAEKNAAMAAWSALKQLAQQSENSSQKVNIDEQEHVTVARALQKYRIRAKMSNTPFPIRYPMPKPRPASTQQSPATTSKILPLICAKPTPWNRTIGTTTNNASTCPKTSSQSRYINPSRNDLLGGSSLPVKPPATSVNEWPTDSSRFLPQKFPAAGAAPYIPLRHFRPHHGMAPPVTIRHAIPAYSAPPFPPIQPVNAMRTPHMGMAPPVSIRQVVPAFAAPVRTEDSPAFRAPLTQVTACAEDLAAFKAPVSTPSARVEDSLPASKVTMPPITPPGQEETAPAFKSHAAPAHIHLENPSTAKVPCHPVSVGAENESNLEFSIKTSTFHAETQQTSELSVRPPLVSHMTAETDKEVESKLPESLMVEELNELKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WH90</accession>
    <name>A0AAF0WH90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0207293</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108208295"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="3-oxo-5-alpha-steroid 4-dehydrogenase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF35">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="139"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG88060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="267" mass="29872" checksum="2E6A9657B3FABD67" modified="2024-05-29" version="1">MVLTRFIYPLPASWFLTAMTVISGAASVIAGLMEIKGKSMQYSKFFNTNNKNVEASNTKVIKFSGRNGMILAYAPAFVASAASVAFMQDKGLRFTLVTSALAFHFFKRLFEVIFIHKYSGSMPIDTAIFISAGYFLSSLSLIYSQHLCGEFAEPIVDLKYAGLTIFLVGVVGNFYHHYLLSKLRKEGEKQYKIPRGGLFHLVICPHYLFEILGFVGISCISQTVYGLSFAAGTICYLTGRSFATRKWYLSKFEDFPNDVKALIPYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN53</accession>
    <name>A0AAF0WN53_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0311972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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                <person name="Senalik D."/>
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                <person name="Bowman M."/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="376" mass="43652" checksum="471F0D5757AFFA21" modified="2024-05-29" version="1">MAGAFDSEHLLAEILKRLPPKSLAQCTCVEKSWYDLIQSPYFKALYSDSNQFIYMLAKLFHRGFALYRMDPLLHPRFSSNSCSSDSTYLHDLRPLLVFPTDSHLGDNYFKCIKLGSKIYFYGLALSSGNKYTSFYTMEEKDLMSIEPSEDNVITNFKHLLTKIKNPMHELKLFPIVFVANDKLYVLSGCFLDNIFEVFSPADGTWQVLPNPEDDALEFHYYADGRVSYVCASKMTSRGEEFMQPYLAPDEAFLEVLRYSRFTYMFKQCSTDYIVAFQDSDGKQQLCIITYGCRPLEKRFSPYETTCYVALSFYDIPGDFYTSEDRHFHEPDVYASYADEKTKDGGVIRRYFSAKFLYTKHFIINNFSFGTIETCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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        <name type="common">Carrot</name>
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    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH03942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20835" checksum="A30CCCBCB700E6AA" modified="2024-05-29" version="1">MNYAAWNVRGLNKRSHQKEVLSFLNSNQLTFVGLLETKIKSCNLAKVVGKLKKNWQWYANHVDHYNGRILVGWDASIWDISILNTSPQHVTYNVGFRASNVHFLVTFVYAFNDGSDRVPLWDTLSAFDPNVPWCICGDFNTVLSIEEILGGREHWTPEMQQFKDCVYDSGLAELRTSGDLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108225799"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="41"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH04667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="157" mass="17751" checksum="BEE893EDBBB9D032" modified="2024-05-29" version="1">MDLRVMGFDPLLNTLYHILDDDNHSANDTNKSTQARTYVRDAKAMASTPADVKEYPNSFVFVVDMPGLKSGDIKVQVEEDNVLVICGERQREEEKEGVKYVRMERRVGKFMRKFVLPENANLETIKAVCQDGVLSVTVDKLPPPEPKKPKTIEVKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEL7</accession>
    <name>A0AAF0XEL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0624998</name>
    </gene>
    <organism evidence="8 9">
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        <lineage>
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            <taxon>Apiaceae</taxon>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000833">
        <property type="entry name" value="Protein TOO MANY MOUTHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053213">
        <property type="entry name" value="RLP29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009:SF16">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042138612" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH05579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="50233" checksum="D0ADAD2CA9A771AD" modified="2024-05-29" version="1">MHCSFSLTLSLLLLLSLLTISSTTQQHGKKNLQPNSMEPSELETLFKIMDSMSSDQTWRVSYPNPCKPGSSWMGIECKAGNDSHLHVTRLDFGSPPSPSCKMNSIFPSLIFHLPYLQSAIFSQCFTHTKTTLTISLDKFFSSKLQQLSLRSNPALIGSIPPQISTLKSLQTLTLSQNRLTGPIPKEICGLTSLQHLDLSYNFLSGTIPNQLGSLKNLVVLDLSYNSLTKLIPGTIGQMGMLQKLDLSSNLLTGSLPNSIQKLNSLVFMALSNNRLKGKIPKGLAKLQSLQYFIMDDNPMFIPLPAEFGALHRLQELRLANSGYSGTIPPIFSQLQNLSTLSLQNNRLVGSIPVGFGNLSHIYHLNLSRNLLGGEVPFTSTFFQRLGKNLDLSGNQGLCLSPSEAYGVKKIGVNICGINKTTSLSKPLKHSEAPSLGITKLFFLVNVFFILFLQQILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH86</accession>
    <name>A0AAF0XH86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0727449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XH86"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd20405">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20406">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917:SF153">
        <property type="entry name" value="DUF724 DOMAIN-CONTAINING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="1">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="1">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH08013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="18048" checksum="7616431D81E6390A" modified="2024-05-29" version="1">MAYNFRAGSRVEVRSDEEGFRGAYYVATVLEDPKFEQCIVVYDQLSESEDPNSRNLTEKVHVSRLRPLPPLSKGEMIKVHDNVDAYYNDGWWPGEIVESLQDGKFLVYFADPPDQQVVERNDLRHHLEWANGKWKKPRRKVFFFLSIFLFNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHB7</accession>
    <name>A0AAF0XHB7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0727259</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHB7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12458">
        <property type="entry name" value="RRM_AtC3H46_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000678">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 53-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056276">
        <property type="entry name" value="AtC3H46-like_PABC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034365">
        <property type="entry name" value="AtC3H46-like_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24009">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24009:SF11">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 53-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23182">
        <property type="entry name" value="PABC_AtC3H46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="345"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="542"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH07825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="73311" checksum="B4539AE644095769" modified="2024-05-29" version="1">MDNNEATSTVLSRIKSLDPENGSKIVGYLLIKEIGEKEMIRLAFGPENHLISLINQVKAEMAAAANTPSASPFIPIANPNRPNPFAQSSPRIIVPNNGFMSPSSPSSPWFHSPKQGASASSLSYAAVVNGAAGNNSPSPFASPSNPSFVSPFFQNGDDEALFLEDGGKNMDFTDPIVSPGGRSDSMLFPFGNCSENHPQFLHRRSVSVNDVFLGQSDESSSNVLGGGFGWKPCMYFAKGFCKNGGGCKFVHGGFGGDSPDGSPKFDGFDELMRMKAIQHQRMAMAAGAPMPFNNRCMSLLNDSPRSAAAAALMMGDDYLPFGRCRHDRSDFAGFGFREHPNSSSRQIYLTFPADSTFKEEDVSTYFSTFGPVQDVRIPYQQKRMFGFVTFVYPETVKQILAKGNPHFVCDSRVLVKPYKEKGKLPDKKHMEIDDLPACFSPSGPSSVEPFDLHFGRRMIYNQEMMLRRKLEEQAELQQAIEFQGRRLMNLQLPEMMNPLHRHMAVMQVPFPAPGYPQMNPGLGHSIETEGINQEELIGVNTGNDEAVSPTMPDDPQDNESNDSNGNGCDSNKAKVPSVNESDPPASNLEHILPDSLFASPTKSQQSLVSSAAEMDDTIPSTSTLPSSNLFPSLTPGRTMASLTLGMMLVSMADMEGFDFSFWVTLGSHFVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIS1</accession>
    <name>A0AAF0XIS1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0727321</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May transport inorganic phosphate (Pi).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIS1"/>
    <dbReference type="KEGG" id="dcr:108196062"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14476">
        <property type="entry name" value="SPX_PHO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034092">
        <property type="entry name" value="PHO1_SPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF4">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="723"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="13">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="12">
        <location>
            <begin position="612"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
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            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH07887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="800" mass="92324" checksum="850AB5AE23FF0AE0" modified="2024-05-29" version="1">MKFSKELSSQMVPEWEEAYINYSYLKTILQDIDHFKNRNKPPAAAVTTNNPAGLKRRLTLYRAFSGLIQRSSSKISSPGPMQDVESQPILVSSVDRSDGQEGFETRFLMSGDEGGEYELVFFRRLDDEFNKVNKFYKAKVAEVMKEADVLNKQMNALIAFRIKVDNPKYDCPDDTVEMSRIASEVEASSEAVALSASTSTNMRTTKAAHMDVIDEDRSSRGALSKEENEDDSEIKPLSSSTEEKSRNRPMIKSDESCRPPPLEILDHVTIRQPVETPRSTIKGFLNVPIQKELKFSKENMSKIEEQLKRAFTEFHNKLRLLKSYSFLNLSGFSKILKKYDKITSRKASASYLKMIDNSYLGNSDEVTKVMDKVEAVFIKHFSNSHRKEGLRILRPTAKRQRHRVTAFLGFFAGCTLSLILALILIIRARRILDKPGRHRYMVTLFPLYSLFAFIVLHMLMYAGNIYFWRRYRVNYPFIFGFNPRTALGFREVLLLSLGLAVLALGSVLANLDMELDPKTGDYKKFTELLPLFLVVLVIVILICPFNIIYRPSRFFFLTCLFHAICAPLYKVVLPDFILADQFTSQVQAFRSFEFYICYYFSGNYKLRDNSCSSNDVYKTFGFIVATIPYTWRLLQCLRRFFEEKDNMQGLNGLKYFATIVAVSTRTAYSLDKSIAWKVVAIITSAIAAVSGTYWDLVYDWGLLDQKSKNRWLRDKLLVPHKNVYYVAMVLNVILRLAWLQTVLDINFTFLHRETQITVVASLEIIRRGVWNFFRLENEQLNNSEKYRAFKSVPLPFTYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJ88</accession>
    <name>A0AAF0XJ88_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0728548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJ88"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="142" mass="16266" checksum="77A58D3CC5D2BE31" modified="2024-05-29" version="1">MTAARGTMGYMAPELFYKNIGGVSYKADVYSFGMLLMEMAGKRKNLNPYVDQSSEIYFPSWIYDQIREGKEIQTEDASDIEKELVKKMIIVAMWCIQMKPIERPSMNKVIEMLEGDLELLVMPPKPLICPQETPGTEDQELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XK52</accession>
    <name>A0AAF0XK52_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0728721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XK52"/>
    <dbReference type="KEGG" id="dcr:108196441"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH09265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43943" checksum="81872C64449F7E09" modified="2024-05-29" version="1">MEQSHPGCDGGAEAIINLKPNSTISIAYHPLFGPHDDMILLELDEKILPDVLDQRISLRGQPDEDAVLCTQSKTYAVKFVGNSNSVLLIPPTDQFPHKSNIKKCTGEEVVASVIKVALGNMELVEIAPKVDKLKLLLSMNPLKLGELTETDDLDEMIDVDTGLYRWNDLTDMIQASDEELRTALNALSAVEINGYWRTVDDNYMDAILNILLQNSILNDWSLDALNEDEVVGVLVSDGFTQKIARHCLQVFGSKVEGDSLWRLDEQKVCVHFARGILKGGKMKMDNFMKEWERKVPERMNVNFDMLEGEVLTETLGIQTWVYAFSISSLPCDPAARFAALFNEHKKWEWRDLNPFIRDLSVPGLSSDALLLKYTRRSQPTPDSEPVFTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKL0</accession>
    <name>A0AAF0XKL0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0729315</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus. In addition to ssRNA and dsRNA, binds dsDNA, but not ssDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with ALY2 and MOS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKL0"/>
    <dbReference type="KEGG" id="dcr:108193135"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000168">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase 56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="78"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="279"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH09855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="428" mass="48302" checksum="B2E21DD108A8B7AD" modified="2024-05-29" version="1">MGEIKDNDAYEEELIDYEEEDEKAPDSATAKPGGEAVKKGYVGIHSSGFRDFLLKPELLRAIVDSGFEHPSEVQHECIPQAILGMDVICQAKSGMGKTAVFVLSTLQQIEPVAGQVAALVLCHTRELAYQICHEFERFSTYLPDIKVAVFYGGVNIKIHKDLLKNECPHVVVGTPGRILGLARDKNLGLKNVRHFILDECDKMLESLDMRKDVQDIFKMTPHDKQVMMFSATLSKEIRPVCKKFMQDPMEIYVDDDSKLTLHGLVQHYIKLTEMEKNRKLNDLLDALDFNQVVIFVKSVSRAAELNKLLVECNFPSICIHSGMSQEERLTRYKNFKEGHKRILVATDLVGRGIDIERVNIVINYDMPDSADTYLHRVGRAGRFGTKGLAITCVSSTSDSEVLNQVQERFEVDIKMLPEQIDTSTYMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKL4</accession>
    <name>A0AAF0XKL4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0729330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKL4"/>
    <dbReference type="KEGG" id="dcr:108196704"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000037">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000006">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000025">
        <property type="entry name" value="Molecular chaperone Hsp40/DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000009">
        <property type="entry name" value="Putative Mitochondrial DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF22">
        <property type="entry name" value="MOLECULAR CHAPERONE HSP40_DNAJ FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="215"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="215"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="53394" checksum="D4DC21C53C481C43" modified="2024-05-29" version="1">MAAALASTPTSFSPIPSFVSERSRCLLLSSSSFFAGGAQIWGQNCFISVSTSFNCTEFNRSRICVKRVRTVVSASADYYSTLGVSKSADNKEIKAAYRKLARQYHPDVNKQPGATEKFKEISAAYEVLSDDKKRALYDRYGEAGLKSGVGAQAGAYTTNPFDLFETFFGSNMGGMGGGFPGMDSAGFGTRGRTTVTKGEDIRYDMTLEFAAAIFGAEKEFELSHLETCEVCAGTGAKTGSKMRICSTCGGRGQVMRTEQTPFGMFSQVSVCPKCGGNGEVISEYCRSCSGEGRIRVKKDIKVKIPPGVGKGSILRVAGEGDAGPKGGPPGDLFVYLDVKEIPEIQRDGIDLYSTVSISYLDAILGTVAKVKTVEGTTELQIPPGTQPGDVLVLAKKGAPKLNRPSIRGDHSFTVKVSIPKRISTKERDLLEELASLNGTPTSRTQSRSKVQQAASVAESQNGSIVEESEESGQDDVWNSLKDLAGTVANGVVKWFKDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMA1</accession>
    <name>A0AAF0XMA1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0729874</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMA1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000061">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1412">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH10405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="81738" checksum="998DC608A3C5C470" modified="2024-05-29" version="1">MEFPQHVLVVPFPAQGHVMPLMKLAYNLAYQGVKVTFANITFVEAKILAAMSEADKEQCPIRLVSYSDGLEACPEERNGPGLITSLKKVMPKNIEKLIEQINQSDNKEPITCVMADLTAGWTLQVATVFSQKQFDELALGLELSGHSFLWVVRPNIVSGSSHEYYPDNFLERIASRGMIVKWAPQEKVLAHPSISCFFSHCGWNSTMEGVSNGVPFLCWPYLWDQFDNKKYICEMWKVGLALDCDEDGIISRHEIKTKINNLVTNDGMKMNAKKLMEDAKKSVSQGGVMDLPQHVLVVPSPAQGHVKPLMKLAYNLAYHGIKVTFANSQFVEANILAAMSEADKEQCPIRLVSYSDGLEACPEERNGPGLITSLEERNGPGLITSLKEVMPKNVEKLIEQINQPDNKEPITCVIGDLTAGWTLEVARKMGLKQVAVWPAGPASLALALRIPQLIEAGVIDENGMCSTKSIPGKPALQKILFDFSLAINQVVKNPIIVLCNTYYELDSSSCAMIPGILPIGPLPAISNLNPCSGSFLSQDSTCLSWLDKRPPNSVIYIAFGSTAVFSQKQFDELALGLELSGHSFLWVVRPNIVSGSSHEYYPDNFLERIASRGMIVKWAPQEKVLAHPSISCFFSHCGWNSTMEGVSNGVPFLCWPYLWDQFDNKKYICEMWKVGLALDCDEDGIISRHEIKTKIHNLVTNDGMKMNAKKLMEDAKKSVSQGGGSYENFKSLVKYLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNQ1</accession>
    <name>A0AAF0XNQ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Diacylglycerol kinase</fullName>
            <shortName evidence="15">DAG kinase</shortName>
            <ecNumber evidence="15">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0729876</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="15"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAF0XNQ1"/>
    <dbReference type="KEGG" id="dcr:108196263"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1_DGK_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000006">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000027">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="18">
        <location>
            <begin position="142"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="19">
        <location>
            <begin position="353"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="246"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH10407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="723" mass="79918" checksum="04DA2AD9095C0BCC" modified="2024-05-29" version="1">MDDDREPGFPSHMVESRLFTVSCFIAGLIGVLTIAYTAFQWRRNINLGWMKAIARSKKNPKTRSKAPVAPHTWVLESLSRGKSLKCCVCLKSMSASQNHGPMASDNHIHYCSICGAASHLSCSSKTLKDCKRVSMSGYEHVMHQWAVRWTEVADQTEETSYCSHCEEPCNGSFLGGSPIWCCLWCQRLVHVDCHGSMSNETGDICDLGPYKRLILSPLYVREVNRALQGRLLSSITQGANEIASSVRASISRSQSKKSKQENVASADTVNNCSVGNSSTESTDNNENRNASLGVEENCNSTVSIEGTDQNQNGGIVKILDSKPSFKRNSSINEKNEFHLTGTEQRYELTDLPSDARPLLVFINKKSGAQRGESLKLRLNLLLNPIQVFELSSSQGPEVGLFLFRKIPHFRILVCGGDGTVGWVLDAIDKQNYVSPPPVAILPTGTGNDLARVLSWGGGLGSVERQGGLCTLLHHIEHAAVTILDRWKVSFNQKEKQLRPPKFLNNYLGVGCDAKVALEIHNLREENPEKFYNQFMNKVLYAREGAKTLMDRSFADYPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWQNEDETYDNFDPQSMHDKMLEVVSIAGTWHLGKLQVGLSRARRLAQGQSIKIQLFAALPVQIDGEPWLQSPCTLTISHHGQAFMLKRAVEEPLGHAAAIIADVLENAETNHVITAAQKRALLQEMALKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPS0</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0831216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH11725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VINORINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH11725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="285" mass="31711" checksum="BAE8499F62A59E0A" modified="2024-05-29" version="1">MSTTCTFIKQWATDSKRLLEGAEEDDHMSTSPSWNSALFFPGKKLSPIPFGIPRAKESVEDLDIITKFLYFSKSDIIRIRDKAKLDSLSEKLPSKVQSVLGIIGKAIIDLSVANPANPKRFMIAQALNMRERMHPPLPKDHCGNMFLFTSAESVADEKGVELPILVKQLSASVSRALEDCKTLLSLGDEGQMMIAHAFNNLTNRTISKPGHSIVCIFSDWTKFPFYETDFGWGLPIWVSASYIPLQNSAILISDKSGEGIEAWVNLNVGDLSKFQNDCNIMEFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPV3</accession>
    <name>A0AAF0XPV3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0830426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="129" mass="14849" checksum="6FABF1E876819DD8" modified="2024-05-29" version="1">MQCCVVFRVHKCSVIPVYDIKVHDRVHDTYKGELEGKDFAYDEGKSLFTVGALSRNKLEFTVVLQDMSSNNPYNFAFTYNCCYLQKQTELQSCTPTDGCPHGSDHKRMRCPYQSKTHKMEISYAAKITM</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="366"/>
        </location>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="403" mass="46215" checksum="C736EE102D341AAC" modified="2024-05-29" version="1">MSYTMLDALNGDRDDWMIRVRLCRMWESANPNTGEKYSLDMILMDEKAVYTVLKKIKVVASKGSYRPLSHGFKLMFLRTTNPKKLEEGVTNIPMHGFEFIRPDMINRRINDKDILSDVVGCLTCVGGIKTVSGGWKNRDMTIMTDYSITATVTLWEKLAELFDTSIYKQDDGPYIIIFTSTQVKKFKDDIGFSTTRNSRIYVNLDTPYVASLRERFSEISTKLEISVDSPPSMLPEEKMFINRMTIKELLVLTVRAEIFGIDNANGWHYIGCKVCFTKATPQDGVYTCKNCHEVIDYPLIMFRIAIKVKDKSGDTTFILFNNIAEPLLDTSANKIVNKSNNNKDIPKEFESLLGKDIIFKLRLNDYNLVEGKHNFTVGEIFEPRQTKRIIKDATKRTELTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTG0</accession>
    <name>A0AAF0XTG0_DAUCS</name>
    <protein>
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            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0831590</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="dcr:108199790"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
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    </dbReference>
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        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
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        <property type="entry name" value="Malectin_dom"/>
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    <dbReference type="InterPro" id="IPR000719">
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF66">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041905673" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="28"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="672"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH12091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113507" checksum="F65B57CE7EF68783" modified="2024-05-29" version="1">MAKALHTLGSTFILPLVLVMLAGLKEGFVEGQSGHLPQEEVKALREIANQVGKKDWNFSVNPCDKDDPNISNWNTTKIKEMPQYNNSVLCNCSYPGDICHVESMFLKGQDLDGIPPPSLAKLLHIKHVDFTRNYLHGNIPPEWASTNLEYISFTVNRLSGPIPGYLGNISTLRYLSLEHNMFNGTVPPELGKLANLINLTINVNYLTGQLPMELSNLTNLEEMRLSTNNFSGKLPDFQSWKQLDKLEIIASGFDGPIPSSISSLSNLTELYITNLNGGASKFPQLENMTKLKTLMLRSCNISGEIPTYLSQFSQLQKLDLSFNNLAGEIHSDLTDLKSLEILYLTNNSLTGNIPQWIKDRNPADNIDLSYNKFSLEPQPCRDSLNLFRSYGNNLTHGNCLDRLPCSEDRYSLHINCGGEKVTIGNRTYEGDEDSVGPAKYDYEEGYWGASTTGYFLWTKEVSNDQYTANNASVLRMNDWKLYTRARMSPVSLTYYGRCLANGNYTVTLHFAEIIFRDNISYQSLGRRIFDVYVQDELKLKDYDIEHEAKGVDKAVKRKINAVVNDKTLQIRFVYTGKGTNAVPTRGIYGPLISAISIESDNPPKLSNSDQKRKIIIAVVVVALGLLLLTFSGISWWKGYLGSRLSREEALKGLDLQTGVFTFQQIKAATDNFAAANKIGEGGFGPVFKGVLLDGTIIAVKQLSSKSNQGSREFVNEIGMISGLRHPNLVKLHGCCTERKQLLLVYEFMENNSLARALFGPETSRLDLDWATRQNICIGIARGLVYLHEESILKIVHRDIKANNILLDKDLNPKISDFGLAKLDEEENTHISTRVAGTIGYMAPEYALWGYLTDKADVYSFGVVALEIVAGKNNMKYRPNENFVCLLDWALNVQENGNLLDLVDQRLGSNYNKEEAVRMIKVALLCTNSSPALRPTMSCVLSMLKGDMRIQKLKLNHTEMHGEDYLKFQGLRDKYGQGIYGSSSAHSQTFGDTSDDIKYGSSSTSGHDLYTVNLQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTY7</accession>
    <name>A0AAF0XTY7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0933825</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTY7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000045">
        <property type="entry name" value="Kinesin-like protein KIN-14I isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF12">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-14H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="40"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="413"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="275"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="783"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="839"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="898"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="497"/>
            <end position="504"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH14306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="956" mass="106108" checksum="6FEADFC6D8A2D08F" modified="2024-05-29" version="1">MESEGILQFSVASVVGDVLQKHGKELHAIDLESRKANEDFVNRSEATHWLRKMIGVVAAKDLPAQPSEQEFRLGLRSGIILCNVINKIQPQAVSKVEAPTDHTVIIPDLKAKSTNQYQENVKKFLLVAEELGFPTFELTDLEKGGNTSNVVETVLAMKSYYEWKERGALGTWKYGEMWDADKQVPRRSTEIPIRRSLSRMSSGVSLDSFSSEGFGLNPTDMSGASPLHILIRDLLCDKKRDEIPIIVENMLHKVTEEFEHRIANQTEQMKQDITDIEGPDSNESISSPGSAESKIEDKESSKYVKEEDFNENYEHDYGGITQNMKQLVEHYQKNCITQSMKQLVEHHQKNLQVLKHDLYTTKADMQNLKMKYQDEVHGLGEHLHKIAHAASEYRKVLEENRKLYNQVQDLKGSIRVYCRVRPFLRGNSNQGSGTVANIDNGKITLITSSKNGKDEHKSFSFNQVFSPSATQEQVFSDMRPLVQSVLDGYNVCIFAYGQTGSGKTFTMSGPDNLTEETLGVNYRALGDLFLISEQRRKTIAYDIGVQMIEIYNEQVRDLLAPDDIRNSSQKGLNVPDANLVPVASTLDVINLMNLGHKNRAVGSTSMNERSSRSHSCLNVHVRAKDLTSGSEFYGCMNLVDLAGSERVDKTDAVGDRLKEAQHINKSLSALGDVLSALANKTAHIPYRNSKLTLLLQDSLGGQAKTLMFVHISPEYDALGETISTLKFAERVSTVELGAAKTNKDTSEVKDLKEQIAKLKASLAKKESENIQQSKLVRMMSAGASISSSNSQGGGNTSSEEDGYLKDNTSAWASPTGSPQSNVKPDSGKWVDRIMVNKPNSRKIYPEQPDKKHIIRSNSSSEYEMSINRYDLATTTDESDIEAAASDNSEPDFAKVTTMTVLGSKNRSPTPRQAKTTPIRTPIPQPSVRKPSNGARSGIPKTGRQPVDARRKQGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVW4</accession>
    <name>A0AAF0XVW4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0933826</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000366">
        <property type="entry name" value="DDB1-and CUL4-associated factor homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033270">
        <property type="entry name" value="VPRBP/DCAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129:SF4">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13129">
        <property type="entry name" value="VPRBP PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="273"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="934"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1804"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="961"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1816"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
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            <begin position="1851"/>
            <end position="1903"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1910"/>
            <end position="1920"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH16382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1920" mass="209679" checksum="F43240CD38BAFF04" modified="2024-05-29" version="1">MEAAAAAADVVAAAPPPEKPEEMNEEDDKVSANELLLIKAQRLMDKITSSPNNPSPSVLHALASILEAQESRYMEHTFDSTVNNVRAPHTIGRLGNLIRDNDDFFELISSKFLSEPRYSISVQAAALRILFSCSLTWMHPHVFEENVLENIHGWIVSDASKSSAEDHNSNNVHGLQKSSEAEMLRTYSTGVLATCLAGGAQIVEDVLKSGLSAKLIRYLRIRTLGEPNRKDVNHSVERKLASSTSVRGKEEARNRFRYVSENFQLNAPRMIDGVTGNQIEEKDSDRNFTRPSYKHRNDGGESPDKLADDDDFAADAEGCEGWHIQDLRERYGKSGDRHGHGRPLREEDFDNGGQDSSERRVSHGSVKLRGRGKINGYVDAKHGLASPGTVIGHECRTMDVTTTTDQNLRSIIDVEDNDGHATDREDYDDCFQECKIGSKDISELVKKAVRAAEAEARAAAAPAEAIRAAGDSAAEVVKTAALEAFKETNDEGAAVLAAANAATTVIDAGNAIEISRALSAVDSNPISQVTEQKKYDETEEFYIPDSSSLENLRERFCIQCLVILGEYVEVLGPILHEKGVDVCIALLQRSSRPEEPKVVLLLSDVLKLISTLAAHRKFAAVFVDRGGMQKLLNVPRVAQTFLGLSSCLFTIGSLQVIMERVCALPSDVLYRVMELALQLLECPQDLARKDAALFFAAAFVFKAVLDCFDAQDGLHRLLNLLHNAASVRSGVRGTFASGGSHNSDQSPPLEVLTSSEKQVAYHTCVALRQYFRAHLLLLVDSIRPNKSNRSVARNVPSVRSAYKPLDISNEAMDATFHQMQKDRKLGPAFVRAHWPAVDKFLALNGHVTMLELCQAPPVERYLHDLIQYALGVLHIVTLVPYSRKLIVNAALSNDRVGIAVILDAANGAGHVEPQIIQPALSVLVNLVCPPPSISNKPTLPSNGQQRAERNIPDPTIPNQNETREQTSDSVLEKRISSASLSTTSASNLSLTPVSAVPSGLVGDRRISLGVGAGCAGLAAQLEQVFHQAREAVRANNGIKVLLQLLQPRVLTPPGALNCLRALACRVLLGLARDDAIAHILTKLQVGKKLSELIRDSGGQSSASEQGRWQAELAHVAVELIAVVTNSGRASSLAATDAATPTLRRIERAAIAAATPISYHPRELLSLIHEHLQASGLATTATTLLKEAQLMPLPSLAAPSSLSHQGSGQEIPSVHVHWPSRNTSCTFYSDKLKSASLDEDFSIKLDSSVSSSKKRSLVFASTLGLQLKYYDSCPASKGNSTMKNLCVPEGTSETPSLISAIKPSGDADNSFKTPNTLLMKRKLSKTVETASFTSRKRVKISDNGIQNADYMTPGSVRRSSLLAESNAFTTPRVLKDSHGRLISGGLLSENLEDISNSAQATPSVHRGLLNDPQSVSTERLTLNSIVTQYLKNQHRHCPAPITTLPPLSLLHPHVCPESRQSIDAPSNVTSRLTTREYRSKYGGIHGSRRDHQFVYSRFRPWRTCRDDSGAMLSCMTFLGDSSQIATGCDSGELKIFDSDNSNILECFTSQHALTALQSHLFEGTQLILSSSSHDVQLWDASSVSIGPRHSFDECKGGKLSNSGNSFAALSLESTRREILLYDIQTCKLDSKLTNRNSSSSGRSHVYSLIHFSPSDTMLLWNGVLWDLREPHPVHHFDQFTDYGGGGFHPAGNEVIINSEVWDLRKFRLLRSVPSLDQTVLSFNGSGDVIYATLRRNLDDLTAALQPRRVKHPLYSAFRTVDAVNYSDIATIPVDRCVIDFATEPTDSIVGLVTMDDPAEMYSSARVFEIGRRRPTDDDSDPDDAETDEEDEDDDEDLDVNQLLEIVGNGGSDMDDMSDDDDAPSELEDDEDEDQDEDDGVFMIDDQDEDGGMLEFVMEGDDSQVDESSSSDTDDDDSEGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AI25</accession>
    <name>A0AAF1AI25_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="61" mass="6804" checksum="50BA6BA30A17B94E" modified="2024-05-29" version="1">MAARKGDLVLYSGDLFPTEARKLPSCPRSHSVNRKEKPTHPSAPLVDRCGFTKRLSTVHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIB8</accession>
    <name>A0AAF1AIB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0102755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIB8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF3">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="20"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG83578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="33607" checksum="8623790C45DA817B" modified="2024-05-29" version="1">MGPAPATLSSVVALILKDDASDWWAFINESTQWQTFIFHLLAALFGILSVTALVQLLRIQLRLPEYGWTTQKVFHFLNFLVNGVRCVVFVFRQDVQNLQPEIVQHLLLDVPSLAFFTTYTLLVLFWAEIYYQAHEECADGLRPSFLIINSVVYAIQIVMWLVIWWNPIPVLVIISKVFFAAISLFTALGFLLYGGRLFLMLKRFPVESKGRHKKLQEVGYLTTICFTCFLIRCIMISFNAFDKAANLDVLYHPVLSFFYYLLVEILPSALVLFILRKLPPKRGITQYRPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJW3</accession>
    <name>A0AAF1AJW3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase BRCA1</fullName>
        </recommendedName>
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        <name evidence="14" type="ORF">DCAR_0105097</name>
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    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108214626"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BRCT_Bard1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ePHD"/>
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    </dbReference>
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        <property type="entry name" value="RING-HC_AtBARD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Breast cancer type 1 susceptibility protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763:SF9">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN BRCA1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14835">
        <property type="entry name" value="zf-RING_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="265"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="12">
        <location>
            <begin position="430"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="12">
        <location>
            <begin position="536"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="223"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85904.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="71216" checksum="087A3A92698FF7F3" modified="2024-05-29" version="1">MAEECQSRRQLNPWLLHLQKLALELKCPLCLELLRQPVLLPCDHIFCNCCIKTSTKFGSECPSCQSKYADQEVRSAPFMESLVSIYRSLDATTVNTLFQAIPCGGEKKLNQSPVSVKISSVNGTKNKLNQVDADGNSSSGTFFPLLAKNCGRNVLNDSAEEEKMLNMIAVKSATPGGVQDKHMEVNGLDGSVFNADGRVANIQHPGTDGSVNPEVNKVEEIDMNQMGQLSPTCTDSLGDMNDNSCDPASSRDAKRQKKSSSEHGGGVCAFCHSSEVTDETGEMIHYANGKLVAGSVKPFSNGIYVHKICIDWTPQVYYEGDYIRNLESELARSSKLKCTACGLKGAGLGCFQKSCRRSYHAPCAIKISGCRWDFDDYLMTCPVHKTVKFPGERSKFRKSAIHKAESIAAPIAPKQANFWASSVTGLKDWVLCGSALSYEEKCYLAKFASTCGATISRSWNPNVTHVIASIDANGAYTRTLKVLMAILHGRWVVTMDWIKACKEMNKPVDEEEYEAVLDTHGCWYGPKSGRLQTLQNAPKLFSGMNFYFSGDFFPAYKDDLQNLVTAAGGSVVKTKEQLATLSCAGQGSSTTLVVYNVDPVQPYTMDKGNSVVLQRHRTAEELATEFGCQVLKHIWILESIAACTLHPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJX6</accession>
    <name>A0AAF1AJX6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0102029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46344">
        <property type="entry name" value="OS02G0202900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG82860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="437" mass="49130" checksum="F0C062CABC8EB83B" modified="2024-05-29" version="1">MEQSAERSPNGHRGFRVQAPLVDSISCYCKVDAGLKTVAGARKFVPGSKLCIQPDIIPRAHKTKSSRRERTRVQPPLLPGLPDDLAIACLIRVPRVEHNKLRLVCKRWNRLLAGNFFYSLRKSLGMAEEWIYVIKRDRDGRISWHAFDPTYQLWQPLPPVPGEYSEAVGFGCAVLSGCHLYLFGGKDPIKGSMRRVIFYSARTNKWHRGPDMLRKRHFFGSCVINNCLYVAGGECEGIQRTLRTAEVYDPNRNRWNSVSDMSTAMVPFIGVVHDGMWFLKGLGSHREVLSEAYIPGTNSWTPISDALVAGWRNPSISMNGKLYALDCRDGCKLRVYDEATNSWRRFIDSKLHLGGSRALEAAALVPLNGKLCIIRNNMSLSLVDVSSPDKRVESNPQLWENIAGKGHFRTLFTNIWSSIAGRNGPKSHIVHCQVLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AK42</accession>
    <name>A0AAF1AK42_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0205345</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AK42"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1185">
        <property type="entry name" value="DISEASE RESISTANCE RPP8-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="151"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="415"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="574"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG86144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="935" mass="107349" checksum="AEFCE83D65730D03" modified="2024-05-29" version="1">MAEAIVSIVVGRLTDLLNEEVHLLDGVKIEIQQVVSELIRMKTFLPHAVSRIHVDDVRILLADVRELAYDAENVVESFLVTSSSARSRTKKIKILRTKMSLIFDRFGKYNIRSTLKSEESKKSPHQTSEKLERFYSHTIFEPDIFIGVHGVVDSLIRHLVDGSDDCYPLISICGMGGLGKTTLAQKIYNHSTIKNHFAGLAWVSISQKWQTKNVLRRILICLTPEKEKEILGFDEAKLVNNLIQVQERKKCLIVLDDIWTKDAWDSIKGAFAAEKCLSKLMLTSRNVDVPKYVHPKGFVHEPGCLDAEQSWELLRFKALPKGGDALDVKRMEEMGREMVEYCAGLPLAIVMLGGILVTKPSLIEWEKVYRDTMLSLERGKELGEVYQHQLHEVLVWSYNDLPPQLKPCFLYLGKFNEDEWIEVETLYQLWIAEGMVLSSDKKNGETVMQVAESYLGELVHRSMVQVKYKDIESSLTKFKSCSLHDLMRDLSLSQAKEEDLYKVIDLREKNHSHLNASVGSRAAATRQLVVYFDEEYTSKQANPSFGKKANQQRYRSMLLFNEFKTRSVPPVLSSDVANFRLLRVFALEEVQFDKQTVSGTLFRINLGRVLGSLVYLRYLSVRKTNLLLLPSIQNLVLLQTLKLDVRNDVLFPPWLSRNIMGKLGRLRHLYLPDWKVHSLGKNSKMRFHGLNKLETLENVNTQWCEVKDLPKLTGLQKLTLRVDDSYDDVEEVMKCLTAIALSSTSCLQYLVLTISQCDLGSRNGPDILRKLLSDHRYNLQELKVIGQLPELAQLFEQQQLHDAPIDLSLIHITRLHLMLSFLEEDPMPVLEKIHTLRELLIHRKAFMGRELVCSATGFPKLTRLVLRNLPNLVKWRVEEHSMPVLSHLMIRNCLKLKELPEGIKFLHSLEELRVEVMPPDFYERLKVVNGAKYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKL9</accession>
    <name>A0AAF1AKL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4216 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0205312</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKL9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025312">
        <property type="entry name" value="DUF4216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025452">
        <property type="entry name" value="DUF4218"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258:SF4">
        <property type="entry name" value="DUF4216 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48258">
        <property type="entry name" value="DUF4218 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13952">
        <property type="entry name" value="DUF4216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13960">
        <property type="entry name" value="DUF4218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4218" evidence="3">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4216" evidence="2">
        <location>
            <begin position="175"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG86113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39394" checksum="09DEFD9BC69D8929" modified="2024-05-29" version="1">MGMLKRAVKNRACVEGSICEAYSFNEISTFVSDYFPDEVLTKANRVSRHDDGGNVELNGRLAVFGLSGRAYGKQKRIFLSEKDLHAAHTYILLNCDEIDEYALTAGSVLPANVLPLAMGPDKDQVSCNGYKVNGYEFHTKAYGFGKRTTNSGVCVQGDSCNELNQVFYGELEEIIELSYKGTYGGHINLFKCRWFDLEKGRRVDRHGIVDIDLHKSAYSNEPFVLPTQTTQVYYTPTPGRKRDRPPVNWQVVIHTPARRREEVIAGDVYQEQTLHKPNVINVEDDEVIQLDDENEAHEIDPELLLVEDDIDTDNEELLTDTDTEIDTDEDDGYESAKEDDSDSDSSDNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKU9</accession>
    <name>A0AAF1AKU9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0103089</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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    <dbReference type="PANTHER" id="PTHR34357">
        <property type="entry name" value="F7A19.14 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07802">
        <property type="entry name" value="GCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01227">
        <property type="entry name" value="GCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCK" evidence="2">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG96915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="16934" checksum="36BE57CEF1513D83" modified="2024-05-29" version="1">MSPPTPDQEPQQNPQADSIQSPIQEPMNTTSPDQEMQQNPQGDTNLEQEEEEEGECGFCLFMKGGGCKDTFVGWEKCIEEAEKNQEDIVEKCFEVTAALKKCMEAHSDYYEPVLRAEKAAEAEAVEQLEKEKEKEEGGVVEGEKAKEEEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZL7</accession>
    <name>A0AAF1AZL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417789</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AZL7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000376">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF15">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL7 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="96"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG98446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="404" mass="44505" checksum="147E3AB8BFFE0BE9" modified="2024-05-29" version="1">MNGEEESYSSNRSKERADLVIIFVVASLSLTALLVALSYYCYISNKLSKRRSLNHHQTPTADSEDNAKAYANVQVTTEKGLQLFTFKQLHSATGGFTKSNLIGHGGFGLVYRGVLQNGRKIAVKVMDRAGKQGEEEFQVEVELLSRLRCPYLLTLVGYCSESSHKLLVYEFMANGGLQEHLYRIGGSSAVSSLKWEIRLRIALEAAKGLEYLHEHITPPVIHRDFKSSNILLDRNFHAKVSDFGLAKLGSDKAGGHVSTRVLGTQGYVAPEYALTGHLTTKSDVYSYGVVLLELLTGRVPVDMKRAPGECVLVAWALPLLTDREKVETIMDPKLEGQYSMKEVVQVAAIAAMCVQPEADYRPLMADVVQSLVPLVKQHRLKAGNCSSFNTTQSPKTSDFSKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2F8</accession>
    <name>A0AAF1B2F8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RuBisCO large subunit-binding protein subunit alpha, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0521496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2F8"/>
    <dbReference type="KEGG" id="dcr:108223572"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH02108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="62122" checksum="055F2824B6D6656D" modified="2024-05-29" version="1">MASANAISTASILSSPTQVSKRVNQLQGQKVNYRPSNGGKNRGRRLAVRAAAKDIAFDQKSRAALQSGIDKLADAVGLTLGPRGRNVVLDEFGTPKVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASILAREIINLGLLSVTSGANPVSIKKGIDKTVLALIEELENKARPVQGRDDIKAIASISAGNDDIIGTMVADAIDKVGPDGVLSIESSSSFETTVDVEEGMEIDRGYISPQFVTNPEKMIVEFENARVLVTDQKISAIKDIIPLLEKTTQLRAPLLIIAEDITGEALATLVVNKLRGIINVAAIKAPGFGERRKALLQDIAIMTGAEYQASDLSLLIENTSVEQLGLARKITITKDSTTIIADAASKDEIQARIAQIKKELSETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSEFVPAIKAKLEDAEERLGADIVQKALVAPASLIARNAGVEGEVVVEKVKASEWEVGYNAMTDKYENLVNAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKAKPKTAAAGVPQGMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B443</accession>
    <name>A0AAF1B443_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Outer envelope pore protein 24, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0625367</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">High-conductance voltage-dependent solute channel with a slight selectivity for cations transporting triosephosphates, dicarboxylic acids, ATP, inorganic phosphate (Pi), sugars, and positively or negatively charged amino acids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooligomers form large rather nonselective pores in plastidial outer membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Etioplast membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B443"/>
    <dbReference type="KEGG" id="dcr:108224970"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034426">
        <property type="term" value="C:etioplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022843">
        <property type="term" value="F:voltage-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034626">
        <property type="entry name" value="OEP24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35284">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 24A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35284:SF1">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 24A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="12" id="KW-0626">Porin</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH05944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23491" checksum="743344DEF43BA778" modified="2024-05-29" version="1">MIKGSLKVKNTNDKNSAGATVAVNAGNVKLRASMTEAIIVNGPSFNGLALSLEKPGSFIIDYNVPKKDLRFQFMNSVKVAEKSINLNYIHFRNDNRTILDGTVVFDSANKVSVNHVLGSGNAKLKYVYLHRGLTTFEPSYDVAKDSWDFAVSRKVHGDGMVRASYQTSKNLLGLDWTRTTKQNGSFKISASFTVGEQLKIPNVSAESSWDFEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4H5</accession>
    <name>A0AAF1B4H5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0625293</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4H5"/>
    <dbReference type="KEGG" id="dcr:108227166"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042052121" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="27"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="647"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="955"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOH05870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="992" mass="109164" checksum="8A8A2F09A1119DDA" modified="2024-05-29" version="1">MLFTSQIYSFCAAVLLMSFLFFVASAQTLPNDEVEALQEIATRLGKTDWKFGADSCNTTGSGGNNSITCDCHNTVCHVVAITFKRQNLAGKLPSSELLRLPSLQSLDLSRNYLNGSLPPEWGLSRLTNITLLGNRLTGTIPKEFGNITTLLELLLDHNQLSGPVPPEIGNLPAIERITLSSNDLNGELPPTFANLTTLKDFRIADTNFTGKIPKFIEKWINVKILIIQGTRLNGPIPSGIAQLTDLFDLRISDLNGTEQTFPPLRGMKSIKRLVLRNCNIIGQLPEYLVDMKNLTTLDLSFNKISGEIPISFAGGLSNTEQIYLTGNMLSGSDPENRMPKGQNNIDLSYNNFTPATFCQQNDINLFASSSTRNISGIVSCLANPPCQQNLYSIHINCGGREVIGDQGSKYEADIAGGLASFSRSGANWGTSNTGHFMDDSNRKFISTNISRLYMDRPELYMDARVSPISLTYYGFCLSNGNYTVKLHFAEIMFTDDKTYNSLGRRIFDVYIQGRLVLKDFNIALEAGGVGKGIVKSFPVTLRTGDLEIRLYWAGKGTTSIPEKGVYGPLISAISVESDSIPSEGKRSVGLILGIVVAAVSGFVLVFAVLWWKGCLWSKDRMNLDLKGLDLHTGSFTLRQIRAATNNFDVANKIGEGGFGPVYKGLLLDGTLIAVKQLSSKSQQGNREFLNEIGMITALQHPHLVKLYGCCIEGNQLLLVYEYMENNSLARALFGPDEQQIELDWATRYKICVGIAKGLAYLHEESRLKIVHRDIKATNVLLDRELNPKISDFGLAKLVEEEKTHMSTRVAGTYGYMAPEYAMRGYLTDKADVYSFGIVALEIVSGMSNTSSRSKKEPSYLLDWAQELKAEGNLKDLVDERLGSEYNEKEAMVIINLALLCTNISPVVRPAMSSVVGILEGRSVMQDYFSDRSISSGRKQPADFADIQQQSYVTSSSGDSQLKAESTTHSWNTVSSTSRTDLYPINFGSNNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4Y7</accession>
    <name>A0AAF1B4Y7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0626227</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4Y7"/>
    <dbReference type="KEGG" id="dcr:108227397"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041918745" description="Subtilisin-like protease" evidence="11">
        <location>
            <begin position="22"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="14">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="137"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="377"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="15">
        <location>
            <begin position="663"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH06799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="770" mass="82559" checksum="EEE2718946A0A220" modified="2024-05-29" version="1">MVVRWAFFLLPTYLAITNVLSSTKIPTSKTYIVQINRSAKPETFGTHVEWYASVIQSVALEGDDNIDTERIMYNYETVFHGVAARLSLDEVQRLQQHPAVKAVHPESVYELHTTRSPFFLGLESADNTDVLSGNLTGHDVVVGVIDTGIWPESKSFSDRGLPPVPGHWKGTCQTGRGFDKAHCNRKIVGARMFYYGYEADVGKINERKVYKSPRDQDGHGTHVAATVAGAPVRGASLLGQAQGTARGMAPGARIAVYKVCWAGKCSTSDIIEGIDKAVKDGVDVLSLSLGAPTFAYQTDSLSIATFGAMERGVFVSCSAGNSGPNADSLANVSPWMTTVGASTVDRVFPSAVTLGTGRKITGASLYKGWKDGSLRKQYPLVYIGSNSSISDPSALCLQGSLNPKIVSGKIVICNRGLTNRVEIGQVVKDAGGIGMILANTYEHGEDLIADSHLLPAIAVGNKEGAAIKKYAMTNSHPTAALSFQGTRVGIRPSPVVAAFSSRGPNYLNLEVLKPDIVAPGVNILAAWTHSLGPSSLVTDKRIVDFNILSGTSMSCPHVSGIAALIKSRHPNWSPAAIKSAMMTTAYIHDNKYNPLADASTGAASTPYAHGAGHINPLKALNPGLIYDLGPQDYFEFLCAQISSTDMELFGNRTCHHTLASLGDLNYPALSVSFPEKGNNSAVTLHRTVTNVGKAVSNYHAVVSSFKSVVVEVEPATLHFTKKHQKLSYKVTISGKKRQTGPEFGHLIWKNKVHKVRTPIVVSRPPVPIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6G9</accession>
    <name>A0AAF1B6G9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucan endo-1,3-beta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6G9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH06428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34266" checksum="3736E81CE313F029" modified="2024-05-29" version="1">MRLYSPDRNALQALRNSGIEVILGVPNDHLHWVASDQRNAHEWVQGNVIDYSNVNFRFISVGNGITPFHSQNARFAQFLLPAMQNIRNALSAFGLQNRIRVSTAIDQSEVLSQSFPPSSGNFRPEVRQFIQPIIQFLVKNGNTPLLVNFHPYFSYIHNKQDIPLQINKGKGNRDARLDYALMRSSDMLVQDAWLRYTNAFDAMVDSVHSAMEKVGGNSLDIVVSEVGWPAAGGPAASNQNAATHNSNLIRHVRNSGTPKRPKKRIETYIFSMFDEDRKSEENLRHWGIFWNNKQAKYRINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6J1</accession>
    <name>A0AAF1B6J1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6J1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="68" mass="7260" checksum="41ECA7D0622DD666" modified="2024-05-29" version="1">MAQTCTFQLYFVMVTVLLIFSALLNISMVGAQDSATAPLPDMDAGNASDLRIYGGSVILAFLIAVLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6N2</accession>
    <name>A0AAF1B6N2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0624580</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6N2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000002">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0229">DNA integration</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="13">
        <location>
            <begin position="570"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="223"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH05166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1054" mass="120747" checksum="79CF8B47F08DAFAB" modified="2024-05-29" version="1">MTGILKALNIEKTTTRDGEVYVNKSPILQSPLVNHFRQKGDDKENQGRMTFSHPKVELTHFKGEEDPRVWLRKCNKFFLFHQVKDSFKCDLVEMYLDGKADTWFQSYKFVKETVSWKEFGEAIVKRFGKKGGVDEQEEFNKLVQEGSVMEYIDRFEELKAVLLCRNANLDESYFVSSFISGLKGELKPMVRLMKPTSLSDAIDIAQLQEQTVDLIVKKHGSKEKWGNTKKPETNQQEVKNLSNGGDKKPISTTSMFKKISPEEFQYRKNNHLCYKCGEKYGQGHQCKNSQYTFMLFDEAKDEDILSACEEVDEDEREGAVTEVSLNSLSSQMARKTITLEGKLKEKTITILVDTGSTLTYLNVEVVKQLGLEVTKGEPIQVRLADGRMVVSSEWVPKVKWKIQDYSFCFDMRVLDIGGWDMIVGVDWMEQYSPLLFDFKKLYLKLNAEGNKDEQMTLQGTVKNASISLIRGHKLKKYNQGLVANQIKQEVSATIEGTGQQQADEAEVPEEIRVLLNKFPEVFSIPTTLPPIRNVDHEIPLQQDAQPFKLKPYRYPHSQKTEIENQVQEMLRSGIIKPSNSPFASPVILVRKKDNSWRFCVDYRHLNKITIKDKFPIPNIDELLDELHGAKLFSKLDLRAGYHQILVKAVDTPKTAFQTHHGHFEFVVMPFGLTNAPATFQALMNQVFQPYMRKFVLVFFDDILIYSPDLSKHVEHLELVLKKLQDNQLYVKKSKCSFAATSVEYLGHMISADGVSMDHSKVESIRNWPVPGNVKELRGFLGLSGYYRRFIKNYGIIARPLTNLLRKDGFVWNEETSKAFSKLKEALCTKPVLAMPDFQKPFVLETDACDSGMGAVLMQNGNPISYLSKSFNSKNKGFSVYEKELLALVMAVTRWRHYLVGNHFIIRTDHQALKYLLEQQLHTSLQYKWLSKLLGMDYEIQYKKGVENTAADSLSRIKEWEGNVEMLTAVTHVQPRWIQQLKDSYEEDDDIKMTIGKVLLQDDSVSEYQYEQGILKKGNKLCVGNKGGVRQNLIDSLHQTALGGALRPKCLFTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCL2</accession>
    <name>A0AAF1BCL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0933939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCL2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH14420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="259" mass="29876" checksum="D4F45D9D14BCAD7B" modified="2024-05-29" version="1">MDIEEKQAEHIAHFVQQASILKGASLSKLIVDATSHPSLFAFSEILALPNILELQGMENSAYLDLLRMFAQGTWSDYKRNADHLPQLVPDQVLKLKQLSMLTLAETNKVLPYDLLMQELDVTNVRELEDFLISECMYVGIVRGKLDQLRRCFEVQFAAGRDLRPEQLGSMIQTLSSWLTTSENLLSTIQEKIKWADSMSELDLKHKKEVEERIEEVKKTLPHKAELEFRGHEEFFSESGGVMDYDEDRTRPKRRRHPMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BE37</accession>
    <name>A0AAF1BE37_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fantastic four family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BE37"/>
    <dbReference type="KEGG" id="dcr:108202113"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021410">
        <property type="entry name" value="FAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046431">
        <property type="entry name" value="FAF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155">
        <property type="entry name" value="FANTASTIC FOUR-LIKE PROTEIN (DUF3049)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155:SF4">
        <property type="entry name" value="PROTEIN FANTASTIC FOUR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11250">
        <property type="entry name" value="FAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAF" evidence="2">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="283" mass="32058" checksum="6FD28DAC95D184A5" modified="2024-05-29" version="1">MSSTIVYQGSNWQHPENKPLRLKLAAPKPLGLETTNTKENKNPDSGAWNFLQMLSDRPKKIFSTETFCPSHKLSQNSLELCTENLGSETGSDDTCDIDILTRAKSSTITRQQGRVLSAKKMSTRSFPPPLTTISGSNSLHVKPRREGGRLIIEAVEAPSSRACFEAERSNGRLQLRFLKNCEFFYDTKARSEDTENENIENFEREDYKTHEIEDVFVGDEFKKDGANNKIVGYIKKDKDGIRQDVEVVMGRESIARHNSRCKESGHGNNKRGFCNWEAFWVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEI0</accession>
    <name>A0AAF1BEI0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BEI0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF52">
        <property type="entry name" value="OS01G0631500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041936097" description="X8 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="3">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH15113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="115" mass="12761" checksum="B46DFCDBD9B11867" modified="2024-05-29" version="1">MAKIISHLCCTLIFLSLVAASQCERLTVPNQIPAPTWCVASDSATDEQLQGFIDFACNGKIDCRFIQPGGSCYDPNTPRGHGSYCLDAYYRIYKDCNQIGTIIYKDPSYGDCLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BFX4</accession>
    <name>A0AAF1BFX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BFX4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF93">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="213"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH15412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="35307" checksum="0398F7CB17E5C6F9" modified="2024-05-29" version="1">MQHRFAFECLDRSLRDIMKSSDPARAALPFGGITVVLGGDFRQILPVIPHGDRAEILGACITRSRLWNICKIFILKRNMRLNKGTSQSEIDEVNEFAKWVLEVGDGNLEGGEEAEKSFKEDGIQVPMKFCDLENDNSVENMIRSIYPDFVENSSNAKYLSDRAILTPTNQTVGHLNSLIVDMIPGDAVTYYSVDNAEEFAGTEDDLQSAFPIEYLNSLSIPGMPAHDLKLKVGVVVMLMRNLNQTLGLCNGTRMIVTKFGLFLPKPVFTHGQFYVAISRVTSPGGLKIFIDDDPISKVLRNAIGNCFEIIHYQILPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BH90</accession>
    <name>A0AAF1BH90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">c-Myc-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0935940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AMY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BH90"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026060">
        <property type="entry name" value="AMY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13168">
        <property type="entry name" value="ASSOCIATE OF C-MYC AMY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13168:SF0">
        <property type="entry name" value="C-MYC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02028">
        <property type="entry name" value="CMYCBINDINGP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH16387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13436" checksum="0B5791B80DB8526A" modified="2024-05-29" version="1">MLRNKEESEKEAKKEAFRKYLESSGVVDSLTKVLVALYEQNVKPSSAIEFIQQKMGGPSLSDYEKLQAEMSDLKIKHKELLASHEEKCRELEELKSSLTMSSSAKETDDMEVLDHNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BLG2</accession>
    <name>A0AAF1BLG2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0936184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH16626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108200353"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF52">
        <property type="entry name" value="F12A21.28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="3">
        <location>
            <begin position="250"/>
            <end position="352"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
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        </location>
    </feature>
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        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
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            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-07-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
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    <name>I1TIC0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5 18">NADH-ubiquinone oxidoreductase chain 6</fullName>
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        </recommendedName>
    </protein>
    <gene>
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        <name evidence="21" type="ORF">DCAR_0100221</name>
        <name evidence="22" type="ORF">DCAR_0100224</name>
        <name evidence="23" type="ORF">DCAR_0M036250</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="20"/>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Plant Biol." volume="12" first="61" last="61">
            <title>De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Senalik D."/>
                <person name="Szklarczyk M."/>
                <person name="Grzebelus D."/>
                <person name="Spooner D."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="22548759"/>
            <dbReference type="DOI" id="10.1186/1471-2229-12-61"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 18"/>
    <dbReference type="EMBL" id="JQ248574">
        <property type="protein sequence ID" value="AEY81150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG81076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH16690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069406.1">
        <property type="nucleotide sequence ID" value="NC_087060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1TIC0"/>
    <dbReference type="STRING" id="79200.I1TIC0"/>
    <dbReference type="GeneID" id="88614213"/>
    <dbReference type="Gramene" id="KZM81318">
        <property type="protein sequence ID" value="KZM81318"/>
        <property type="gene ID" value="DCAR_032460"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614213"/>
    <dbReference type="OMA" id="PHRETRT"/>
    <dbReference type="OrthoDB" id="1914986at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1200:FF:000003">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005164">
        <property type="entry name" value="PRK06638.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269:SF17">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 18 20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 18" id="KW-0520">NAD</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0691">RNA editing</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 18" id="KW-0813">Transport</keyword>
    <keyword evidence="14 18" id="KW-0830">Ubiquinone</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010124883" description="NADH-ubiquinone oxidoreductase chain 6" evidence="19">
        <location>
            <begin position="21"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AEY81150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG81076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG81079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOH16690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="205" mass="23463" checksum="FAF4FCF93AF2D25D" modified="2012-07-11" version="1">MILSVLSSLALVSALMVVRAKNPVHSVSFSIPVFRNTSGLLLLLGLDFFAMIFPVVYIGAIAVSFLFVVMMFHIQIAEIHEEVLRYLPVSGIIGLIFWWEMFFILDNESIPLLPTQRNTTSLRYMVYAGKVRSWTNLETLGNLLYTYYSVWFLVPSLILLVAMIGAIVLTMHRTTKVKRQDVFRRNAIDSRRTIMRRTTDPLTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WST9</accession>
    <name>A0A161WST9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_010499</name>
        <name evidence="6" type="ORF">DCAR_0311941</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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                <person name="Zheng Z."/>
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            <tissue evidence="3">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG87371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="325" mass="33732" checksum="B10CE1CE2E352221" modified="2016-07-06" version="1">MTSSSVMDLTLHVFLLFTVVAPSGARDAVPLTSLPSPTPSSNTASSSVVTTVPAISDLHESPSSQVLAGAATTIADTGNLGDSASTSYRSADDDEDLPYFSLEAPGNSPSASSPRSLLDDGVIFRQDESSTDAGVVSEREDVPFIRVELLESASVSKSGSASAKDDGVSKSRSADANDDAVSKSRSAGDTYASSSQSGAGGDSPSASSPRSLLDDGVIFRQYESSTAAGVVSERKYVPFMRLERLEAASDSVLSRSASDSVLSQSGADDDLTTILDDSTVVPPRSGYSVSFEDIGSSFASLYRRYKSTPKKSAEQPTDKAAEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YCG1</accession>
    <name>A0A161YCG1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0623844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YCG1"/>
    <dbReference type="Gramene" id="KZM90299">
        <property type="protein sequence ID" value="KZM90299"/>
        <property type="gene ID" value="DCAR_022336"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224453"/>
    <dbReference type="OMA" id="NCVIDAV"/>
    <dbReference type="OrthoDB" id="1900300at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000152">
        <property type="entry name" value="Trihelix transcription factor ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF7">
        <property type="entry name" value="SEQUENCE-SPECIFIC DNA BINDING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH04435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="325" mass="36022" checksum="247520B10F5EF582" modified="2016-07-06" version="1">MDTDIKQETTATNDSQRKLLGGSSNDRIKRDEWSEGAVSSLLEAYETKWVHRNRAKLKGQDWEEVAKFVSCRGNSTKSPKTQTQCKNKIESMKKRYRSESTAAEASSWPLFPKLDHLLRGSITTQRAPVLPQPTLFSSGVMVTEPTLLLASQLPPASPKEPLSLLTPPPLASPINTGIAAANSHGSDGADQVAKADGMDTKVSDHLSDKQAIDTDSSTPALCSNKEKTKSENFTNKTQRKKRRRRGEWEIGSSVRWLAEVVLRTEQARVDAMRDLERMRAESEAKRGEMELKRTEIIANTQLEIAKIFANMGKGVDSSLRIGRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZLA5</accession>
    <name>A0A161ZLA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrator complex subunit 4/Protein SIEL C-terminal Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_023994</name>
        <name evidence="5" type="ORF">DCAR_0727511</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZLA5"/>
    <dbReference type="STRING" id="79200.A0A161ZLA5"/>
    <dbReference type="Gramene" id="KZM86860">
        <property type="protein sequence ID" value="KZM86860"/>
        <property type="gene ID" value="DCAR_023994"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193689"/>
    <dbReference type="OMA" id="SMARDMS"/>
    <dbReference type="OrthoDB" id="18190at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010496">
        <property type="term" value="P:intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057412">
        <property type="entry name" value="INTS4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938:SF0">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25458">
        <property type="entry name" value="INTS4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 4/Protein SIEL C-terminal Ig-like" evidence="3">
        <location>
            <begin position="781"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM86860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH08074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="900" mass="100757" checksum="3C0A167DA34483F2" modified="2016-07-06" version="1">MEQQLLRSFLELSATDEPPSRHTLTLARSLITNPSTSDSTISTILQALTHSLSLNPNPHTISLLSDISLRHPHLRRLIFSSLRTFSLLPHNSPRLVVESLSILVSIAEQDSELLQLVEELSESVFLSVCFGGCVSARRWLLANAKRILVRPAVLVTVFLGFSKDPYPEIRRVALDGLSCCVGIQDRGVMEGCFLRGVEMLSDTEDSVRCSAVRVISEWGQLLTASSEGASKKYWSDALFEKLCSIVRDMSVKVRLQVFDAIGKITMASENILLQTLSKKVLIKTQTSDVAGAFVHGMEDEFYEVRRSACSSLRELFILSSDFACKALNLLVDMLNDDSIAVRLQALQTMHHMAQHDHQVQEEYLNLLFGALFDTSSSIRSEARRIIQLLKMHSSKMFKFCVNGLVKNMEAFPQDEADIFLVLFIIGRKHGSYAASTVEETSQDIDDFFEGKLGSNGAKTALILVLAISASLSHEINICRIPPRMFSYAVTLLGRISSGLAYALDRHVLFSYLCACSRSTSVSDSELIKGDEILQHIVNDSSRNQESREQSSSEVSIADCQLEGIEVLALVNLVLAQISNMWQPMQVGCIDGVLKTLRSLKEELGSINNTSSQSSGVLVFALKYIRVLELFGKIWTNLMCLKKSPLTGEIEINFGELDYKLRELAYTFLGLDKEVTIHLLELMVVTCTLKLCSLQPWCYVSTLRKLHSIYSVVELLLDKGSIEPSHFLAEAGKSLHGVQTSIGGMSNLFQFRKLLELFSLKQVELAGGLKHVKAELDIINNTCETPLFFVSGLPVGIRLQITLYNMDVDNRLWVKLTVNEYSCQYVYLDLKQFGDSDPVKKFVFAAPFYRTPKTNCFKLRVTLGMEDLSGEKVIVRNFRHPEHDLIYLCTEKEVFLSRIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZPR8</accession>
    <name>A0A161ZPR8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF642 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_025475</name>
        <name evidence="9" type="ORF">DCAR_0729286</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZPR8"/>
    <dbReference type="STRING" id="79200.A0A161ZPR8"/>
    <dbReference type="Gramene" id="KZM88400">
        <property type="protein sequence ID" value="KZM88400"/>
        <property type="gene ID" value="DCAR_025475"/>
    </dbReference>
    <dbReference type="OMA" id="HEEHPAC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000031">
        <property type="entry name" value="DUF642 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006946">
        <property type="entry name" value="DGR2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052437">
        <property type="entry name" value="Pectin_Meth_Modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265">
        <property type="entry name" value="OS02G0527500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265:SF61">
        <property type="entry name" value="PROTEIN DUF642 L-GALACTONO-1,4-LACTONE-RESPONSIVE GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04862">
        <property type="entry name" value="DUF642"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831036" description="DUF642 domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="30"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="203"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM88400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="378" mass="41434" checksum="FD374131BC4F509B" modified="2016-07-06" version="1">MQRMKHMLVLVLVVLCASAKPHAALSIIDGLLPNGNFEFGPKPQQLKGTKVMDPHAIPNWETSGYVEYIKSGQKQGDMLLVVPEGAFAVRLGDEASIKTKVKVVNGMFYALTFSAARTCAQEEKLNVSVSPNSEEKDWGILPMQTMYSSDGWDSYSWGFLAESNQIEITIHNPGMEKDPACGPLIDSVALKTLTSPKRTRANMLKNGNFEEGPYIFPNTSWGALIPPNIEDDHSPLPGWMVESLKAVKYIDIAHFAVPEGKRAVELVAGKESALAQVVKTTPGKVYDLTFSVGDAKNSCEDSMIIEAFAAKSTLQVPYQSTGKGGFKKAKLRFKAVSPRTRVRFLSTYYHMKSDHSGSLCGPVIDDVKLLSVRYPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ALM1</accession>
    <name>A0A162ALM1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARM repeat N-terminal plant domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_011679</name>
        <name evidence="4" type="ORF">DCAR_0313188</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ALM1"/>
    <dbReference type="STRING" id="79200.A0A162ALM1"/>
    <dbReference type="Gramene" id="KZN02923">
        <property type="protein sequence ID" value="KZN02923"/>
        <property type="gene ID" value="DCAR_011679"/>
    </dbReference>
    <dbReference type="OMA" id="CIESQGT"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058868">
        <property type="entry name" value="ARM_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46578">
        <property type="entry name" value="ARM-REPEAT/TETRATRICOPEPTIDE REPEAT (TPR)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46578:SF2">
        <property type="entry name" value="ARM-REPEAT_TETRATRICOPEPTIDE REPEAT (TPR)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26524">
        <property type="entry name" value="ARM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARM repeat N-terminal plant" evidence="2">
        <location>
            <begin position="6"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN02923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="609" mass="67949" checksum="00D610DB5D3B430A" modified="2016-07-06" version="1">MDKISQDCPYPGCFFCVMKEGNPSKRRASILKFFRELPSQDDDGQVLPISGLWNTAMAHPNDPEFIELGIFECMTALIWKGLKNRRWLSHDQNIYIPYYAAHIIGSYTMNMEEFAESAVHAGVVPALVELLRGRLTWVEQRVAVRALGHLATYASTFPAVASHGEILELSIQLAMSSLEIVYSHFYQNADRRLSYHCDLLTRGMGGVEMESRKAEEWASQLQCWSLQLINCFAFKPEFLSTICKQDFLIKLPGMWGGLVNENSPAGIGLLRTICHHKVGKGPVASCPGMIEALCNIARSSDDWQSMAIDCLLWLLQDPTTCHKVIDTAVPALLDLADISTLGDHKKLGDSIVNALQECIPSLGTARNSVSRNSVSDQIKDEIEEILSSRQRFKWEKNLPKEDLHIKQAASLVVKLEGNSLFSAGNISGAASKYSEALSLCPMRSKKERVVLFSNRAQCRLLLQQPLAAISDATRALCLHNPVNRHAKSLWRRAQAYDMLGLAKESLLDAILFINECSQSTDPDLSLRQNKVPDYAERLVKKQMREAWLFKEAAIKHGGVHMEGHGGEVYGQESDDSEWETASESDIGNDGRDEMGENDKARYDKPSKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ANY7</accession>
    <name>A0A162ANY7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_012549</name>
        <name evidence="3" type="ORF">DCAR_0314219</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ANY7"/>
    <dbReference type="Gramene" id="KZN03793">
        <property type="protein sequence ID" value="KZN03793"/>
        <property type="gene ID" value="DCAR_012549"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212905"/>
    <dbReference type="OMA" id="CKVEDAM"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056794">
        <property type="entry name" value="PATL1-6_C_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF3">
        <property type="entry name" value="PATELLIN-4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25099">
        <property type="entry name" value="GOLD_PATL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="139"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN03793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="432" mass="50657" checksum="C423CDE2DC6787D1" modified="2016-07-06" version="1">MVHEGKVHPESTYKDEAIYNVHEIDQEKAYKEYHIPLAMTISKKKVLYEFRSKVEDAIQNGYILKKETFHYEHAKKDEEYLKELKIWGVPLLPSKGHEATDAVLMKFLEARCFKVSEAFHLLQRTLTWRMEIDMEELLQEKLGSDLDRLGRIGDTKAIKGQSLCYKFYGIYKDKDLYKERFNSDDKYEDYLRWNIQFIERCVQTIDFKPGGTNSIILIVDLKDAPTPLIKELPNLHKKTLALFQDYYPGIIYRHLILNAPLWLLTFHALQVRTLTNRNYGKFIFVRPFRVSKTLLKFIAPEDLPVQYGGLKQESDELFPSNHKILNRRVKSGTKECIQIPVNKAGMTVLWDLTVVGFEVSYKVEFVPDDDCSYQILIQKEKKLGKTIRNSFHVREPGKIVIQIENATYTNKTVFCRHLIQPTVTNFTWTKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ARK9</accession>
    <name>A0A162ARK9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0206398</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TJB5"/>
    <dbReference type="Gramene" id="KZM87601">
        <property type="protein sequence ID" value="KZM87601"/>
        <property type="gene ID" value="DCAR_024721"/>
    </dbReference>
    <dbReference type="OMA" id="HTIKYST"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH08953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="395" mass="45943" checksum="79B2A708E45D9DB7" modified="2016-07-06" version="1">MSDSNGDSFGKVKQRLKDRTKKVVETKEMLSKQAVKSREILSKQAVKSKQMLSKQAQNIAKQAQEHERFINKVTHFVGVIGFGTFCFLLGARPQDIRYVYCLFYLIFVPLRWIYYRYKKWHYYLLDFCYYANTIFLVMLLFYPENEKLFMVTFSFAEGPLAWALIVWRCSLVFSSVDKLVSVLIHLLPGLVFFTIRWWDPAFFEAMHPEGTVRRASWPYVEDRSYLWTWLFVVPLAAYSIWQVLYFLIVNVLRHQRLLRDPEVMTSYRELSKKARKANNLWWRLSGLLGDQNRMFMYILLQAVFTVATMALTVPIFLSYELHCIFQILKVSASVWNGGSFLLEVMPRQAVMKEKKKSGMQPIQPQEAQSSVASETLTEAPATAVDAVIVGGTPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WDP2</accession>
    <name>A0A164WDP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Proton pump-interactor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_020991</name>
        <name evidence="14" type="ORF">DCAR_0625342</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the plant Proton pump-interactor protein family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WDP2"/>
    <dbReference type="STRING" id="79200.A0A164WDP2"/>
    <dbReference type="Gramene" id="KZM91644">
        <property type="protein sequence ID" value="KZM91644"/>
        <property type="gene ID" value="DCAR_020991"/>
    </dbReference>
    <dbReference type="OMA" id="HESIELK"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055282">
        <property type="entry name" value="PPI1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219:SF2">
        <property type="entry name" value="PROTON PUMP-INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32219">
        <property type="entry name" value="RNA-BINDING PROTEIN YLMH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="397"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="533"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="248"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="470"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM91644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH05919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="72256" checksum="6F08AF536BA067F8" modified="2016-07-06" version="1">MDSTETELAHVSVEATTEGNISLHKENGILNPDSGITFGSHGTEETGKKAVNNLPENGIPKNAVDEWPEPKQIHTFHLVKYRLYDDQKTKFLLDQADKELKKINQARYLITEKLRAKRADRAQVISQLRALVDESKQFRQIIDEKRKEIEPLHQALGKLRSSNTGDRERSFICSSEEELNNVIKSLQYRIQHESIPLSEEKQLIREIKQLEGTRDKVIAHAAVRAKIQDSLGEKESIQDQVKLIGSGLDGVRKEKKVVQDKLDQLDKEKEAINKVIKDLDAEMDLNTENREKAYEKIRELRKQLDEGNAPYYQNRTTLNKARELAARKDVEGLQNLAATEVETFMSLWSTTKAFRDDYERRILPSLDMRQMSRDGRMRNPDEKPLVVRENFPIAETERVVKPQQKPLKEDSNTSINQKEKSAKQQKETSKVPDSEITVAATVVKDEFEKIEKDPLPVINKVEKDPLPVKNKVDEAKLKELKREEEIAKAKQAQERKKKLAEKAAAKAAIKAQKEAEKKQKEITFLPSCFKNREKKEKKKAGASASVETEEPTEAPEDVVGETETADEVKAVPKSKERKEKMAVRQRGGGRAKGPDSLPKIILKRKKATNYWVWAAALAAILVVLLSVMGYYYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WVA8</accession>
    <name>A0A164WVA8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0626084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WVA8"/>
    <dbReference type="Gramene" id="KZM92305">
        <property type="protein sequence ID" value="KZM92305"/>
        <property type="gene ID" value="DCAR_020330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="246" mass="28371" checksum="859496DBA2571D12" modified="2016-07-06" version="1">MIGNLRLSTTVAWRLKVRVTRMWRSIDRYGQTVAINLIFVDELAGRIHARIPAENINMLENLFIEGETYHVRNFVVRQYGAMHTERCFRNDVYIQMFNMTQIFPTGVVEHIPHHVFQFTELSAIINAALEASHLIDVVGVMEEVHPILTYTNKYNQQKSSIKFTIKDITGLAEVLFHDELAQSFQQGVNDAHQHPIIVIISSCKANFIQGEPKLSNLSATRFFINHDHEAVDDLRNAVRLANWRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XG75</accession>
    <name>A0A164XG75_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0518738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XG75"/>
    <dbReference type="Gramene" id="KZM93137">
        <property type="protein sequence ID" value="KZM93137"/>
        <property type="gene ID" value="DCAR_016382"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG99390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="179" mass="19455" checksum="F5C4DD7138A6FF4E" modified="2016-07-06" version="1">MGNNRRKTVTIIDHSLLSPPKLASALSFNHSFLDVLEEVYSKVFFWGTEIGPHFAIVSLGPLFMLDVVLQVVPLWRIEDQAGNAAVLLTLGFLPGMRVAAVTMSQSKKYSGTFMDPVPENHGGSGSSGTLLTVLYEIIISESPTVDVDAPAFRPEQFVRDRCEKIKVVLVAVGPHTGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XRH0</accession>
    <name>A0A164XRH0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XRH0"/>
    <dbReference type="Gramene" id="KZM93505">
        <property type="protein sequence ID" value="KZM93505"/>
        <property type="gene ID" value="DCAR_016750"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223433"/>
    <dbReference type="OMA" id="VTYGTIW"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13846">
        <property type="entry name" value="CuRO_1_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13872">
        <property type="entry name" value="CuRO_2_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000029">
        <property type="entry name" value="L-ascorbate oxidase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000012">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000016">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034273">
        <property type="entry name" value="CuRO_1_AAO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034271">
        <property type="entry name" value="CuRO_2_AO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF387">
        <property type="entry name" value="OS04G0561900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="540" mass="59997" checksum="EF81141ABA32C68B" modified="2016-07-06" version="1">MWVDSAFLLSLCVALVGIVGAEDPYRFFNWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVFNSLDQPFLLSWSGIQQRRNSFVDGVYGTTCPIPPGRNYTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIRILSRPRIPVPFDEPAGDYTVLIGDWYKANHTDLRKHLDAGKKLPFPDGVLINGRGPNGLSFNVEQGKTYRLRICNVGLEHSLNFRIQGHKMKLVEVEGTHTLQTSYSFLDVHVGQCYSVLVTADQPSQDYYIVASTRFTTQILTGIAILKYSNSAGPVSGPPPGGPTIQVDWSLNQARSIRTNLTASGPRPNPQGSYHYGMIPTSRTIRLSSSAGQVGGKQRYGVNSVSFVPADTPLKLADYFNIQGVFRVNSISPNPTGGGLYLDTSVLGADFRAFVEIVFENTEDIIQSWHIDGYSFFVVGMDGGQWNSGSRNNYNLRDAIARTTVQVYPMSWTAIYVALDNVGMWNVRSEFWARQYLGQQFYMRVYAGTNSIRDEYPIPKNARLCGRAAGRHTRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z7V5</accession>
    <name>A0A164Z7V5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0521431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z7V5"/>
    <dbReference type="Gramene" id="KZM95487">
        <property type="protein sequence ID" value="KZM95487"/>
        <property type="gene ID" value="DCAR_018729"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223446"/>
    <dbReference type="OMA" id="WPTENED"/>
    <dbReference type="OrthoDB" id="1938526at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46872">
        <property type="entry name" value="DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46872:SF10">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH02043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="50616" checksum="66F721B25674C87C" modified="2016-07-06" version="1">MDEKMGRVSVSGVNHAVKQKFVFGKYPEKEWLMKLKALAVNHHTPGITTNSIRPLWDQTLRVREFMALTNFPQRKRKVQQFLKGNEKREAQKLCDEQREAEYVRGQLLRASSASCVHKLNMTDSIENLHKRIHLNSLSSGSLLTSKDNIESLPYVSCIMDNVKDWYHSKKITSLIVDITGSIYTSNPVTPEHINLQEQQSLHLEKGRNSLNLEEARYDCLPSRSNRLVDFFGYQIKRMAVPVGQHFQADVSEWNGLSVNSDFDNSRWLGTKVWPIKMQNVKATSRSVGKGRPSSCSCASPGSTNCIRHHILEKRRLLQSDLGPVLHIWKFDEMGEEVSRSWTVKEQEGFDLIAKRKSSSSNFIQNAMKFFPSKCKEDITRYYFNVFIPRFMSSQARSLLKEVDIDIDDVDDVYTLNWRRSHEDRSRSYQRSCKDVISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZJW8</accession>
    <name>A0A164ZJW8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0522051</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group II-b family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZJW8"/>
    <dbReference type="Gramene" id="KZM96025">
        <property type="protein sequence ID" value="KZM96025"/>
        <property type="gene ID" value="DCAR_019267"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220915"/>
    <dbReference type="OMA" id="PSPRQFM"/>
    <dbReference type="OrthoDB" id="2020995at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000002">
        <property type="entry name" value="probable WRKY transcription factor 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF50">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH02662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="593" mass="65071" checksum="40678C1069A43515" modified="2016-07-06" version="1">MAQGSGLSIDPPSSHLLFTCSLSSDHPQDLSLRHRHKLRLEPSIQQLMDCPKGPSTIQFPVNLNCTTADHDHDQDQERDEDRCFKSDEKRPVIDEMDFFASKKDDEDHDHIAETDGDDQKDGLCGPQHFNVNTGLHLLTTNTSSDQSIVDDGVSSSSEERRVKSELAVLHAELERMNSENQRLRDMVDEVTNNYNNLQMHLVTFMQQQQQQKADDNEVEADDDGGNNSKGEKFVRQNSMRNTVPRQFIDLAVGGGSNDETTDQEPSLSSSEGRDPSHSRMSKQKQDDLYRETDSPNHGSPHWGGSNKVSRLNPSKEVDQATEATIRKARVSVRARSEAPMITDGCQWRKYGQKMAKGNPCPRAYYRCTMAAGCPVRKQVQRCAEDRTILITTYEGNHNHPLPPAAMAMASTTSSAARMLLSGSMPSADGIINSNLLARTLLPCSSSMATISASAPFPTVTLDLTQSPNPLQQLQRPQNQFQLPFANPQQSPNSPAALLPQIFTQALYNQSKFSGLQMSSHDTDANQALGFPPMHNQAQPGTMADTVNALTSDPNFTAALAAALSSMFGGTSHANNSTNNNANVTTSTSNNNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZM47</accession>
    <name>A0A164ZM47_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_019356</name>
        <name evidence="2" type="ORF">DCAR_0522152</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZM47"/>
    <dbReference type="STRING" id="79200.A0A164ZM47"/>
    <dbReference type="Gramene" id="KZM96114">
        <property type="protein sequence ID" value="KZM96114"/>
        <property type="gene ID" value="DCAR_019356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896:SF2">
        <property type="entry name" value="CBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM96114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH02763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="16043" checksum="49611B9699449B32" modified="2016-07-06" version="1">MTTYFNKTKWPEKAPKSEEEQKDFIASLHKRKTELFMVLIEKKLLPLRPGVTNFQNNIWCSDSTQTSKPWSKEFNSSTRYVNARAEAATEAHKFTAQKKFCNVSVQIHPHQVTLQTWGYKENTPSCYIERMIQPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A4C9</accession>
    <name>A0A165A4C9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_015742</name>
        <name evidence="10" type="ORF">DCAR_0414966</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A4C9"/>
    <dbReference type="STRING" id="79200.A0A165A4C9"/>
    <dbReference type="Gramene" id="KZM96896">
        <property type="protein sequence ID" value="KZM96896"/>
        <property type="gene ID" value="DCAR_015742"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216676"/>
    <dbReference type="OMA" id="DHICTKC"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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</entry>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Zheng Z."/>
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            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
        </source>
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    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="74"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM97106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="281" mass="30168" checksum="608677934695CA6E" modified="2016-07-06" version="1">MGKEEVSEQTHEYSGKDYQDPPPAAFIGLDELGKWSFYRAVIAEFIATLLFLYITVLTVIGYKSQSATDQCGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVMYMVAQCLGAICGVGLVKAFQKSYYNKYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKDKAWDDQWIFWVGPFIGAAIAAFYHQYILRAAALKALGSFRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WR09</accession>
    <name>A0A165WR09_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein bL12c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CL12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0415764</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WR09"/>
    <dbReference type="Gramene" id="KZM97585">
        <property type="protein sequence ID" value="KZM97585"/>
        <property type="gene ID" value="DCAR_015053"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215802"/>
    <dbReference type="OMA" id="FFPIHGR"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF26">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12CX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG96429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="185" mass="19490" checksum="270D8A4345D72A25" modified="2016-07-06" version="1">MASSTPSTLLVPLQRSSSYSHPVNSSISYPTHPNSLISSPKHQRNFLRPVSAVEAPEKVVELGDKISDLTLSDAQKLIDYLQETLGVSAASLAPVAAVAATGAVADAPAAAEEKTEFDVVIDEVPSNARIATIKAVRAIITSLALKEAKELIEGLPKKFKEGVSKEEADEAKKQLEEAGAKCSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z3V9</accession>
    <name>A0A165Z3V9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_013060</name>
        <name evidence="9" type="ORF">DCAR_0418034</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z3V9"/>
    <dbReference type="STRING" id="79200.A0A165Z3V9"/>
    <dbReference type="Gramene" id="KZM99578">
        <property type="protein sequence ID" value="KZM99578"/>
        <property type="gene ID" value="DCAR_013060"/>
    </dbReference>
    <dbReference type="OMA" id="DCILMQD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF11">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007869743" evidence="7">
        <location>
            <begin position="26"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM99578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG98690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="110" mass="11776" checksum="0B49450EF0777764" modified="2016-07-06" version="1">MAYHYGKVALFLLAALVGKEHGVVAGLAGCGLIKSVVSVDCILMQDLKTGHLILTSPRAMLLRQSIGTTIGCMVAPLSFMLYYKAFDVGNPTGEFKAPYVVIYRSMAFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A694</accession>
    <name>A0A166A694_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="9">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0310786</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="9"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A694"/>
    <dbReference type="Gramene" id="KZN00788">
        <property type="protein sequence ID" value="KZN00788"/>
        <property type="gene ID" value="DCAR_009542"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215400"/>
    <dbReference type="OMA" id="QHLYNRQ"/>
    <dbReference type="OrthoDB" id="25308at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
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        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF39">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
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        </location>
        <ligand>
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        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="335" mass="38012" checksum="4B8937FE15486DAC" modified="2016-07-06" version="1">MRMRDEIVDYQGEEEDETVSFYPPAKKGDVVVVGYALTSKKIKSFLQPKFQDLARKKGIVFVAIDQSMPLSEQGPFDIVLHKLSGKEWRKVLEDYRQMHPEVTVLDPPDAIQHVYNRQSMLQDVADLDLSDTYGTVGVPRQLVIKKDPSSIPDAVSKAGLKLPLVAKPLVAKSHELSLAYDCYSLEMLEPPLVLQEFVNHGGVLFKVYVVGDAIKVVRRFSLPDVSKRELSKSAGVYRFPRVSCAAASADDADLEPCVAELPPRALLERLSRELRRRLGLHLFNMDIIREYGTLDRYYVIDINYFPGYGKMPGYEHIFTDFLLSLVQSNKYKKHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
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            <taxon>Magnoliopsida</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="193"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20429" checksum="2BB55422F0717016" modified="2016-07-06" version="1">MASSALLALALALAFCIQGSLGNVICENLSKDACAFAVSSEGKRCVLEQRMCRSGMGKYTCRSSEIEADTLKDWIESDECIKACGLERNVLGISSDSLLESQFTQKLCSTPCYNSCPNIVDLYFNLAAGEGVYLPKLCEARGVNARRGMAELKSSGLVSPGPVASERLGEAPGPISTEMFVEAPEPAMGPASL</sequence>
</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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            <tissue evidence="6">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CCX2"/>
    <dbReference type="Gramene" id="KZN03600">
        <property type="protein sequence ID" value="KZN03600"/>
        <property type="gene ID" value="DCAR_012356"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212861"/>
    <dbReference type="OMA" id="AFLFSCW"/>
    <dbReference type="OrthoDB" id="539709at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116:SF9">
        <property type="entry name" value="OS08G0346600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116">
        <property type="entry name" value="PLASMINOGEN ACTIVATOR INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="365" mass="41056" checksum="320FCEE7C267675A" modified="2016-07-06" version="1">MRKILFDPIGTTLPPQLPPSPAPAPSPSEPYYQQSNDFYGSVYNYFLPPGLPEDSAYEMSTISIVICLLILSICSLLFIFHLRLRSRWSHHLQKFSSAWSLRVLLVMYVALWASNEILRLPFFRRRYIYPYLPPLNLAQEVNLCKAYVVLSLGLYEPAILATLLFLLRVSVKKRCPSQLSAIAKVGSMCLPVFLGQILVVYFSPLQAQLPEIFYRGSVISKDLHGNTNVYCTYPWSSTIVFACFGMSYTLGFSLSCWEVLSVVINKSIIYRINFLAMTINSTIPLQIVFLGLSAFSSTGKVASEAMLIAMFFCAALCVIVGLGTLVIKPTADALASVVDFHRSNARVHIRDLEFDKKQEENEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EXP0</accession>
    <name>A0A166EXP0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_007939</name>
        <name evidence="14" type="ORF">DCAR_0208977</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EXP0"/>
    <dbReference type="STRING" id="79200.A0A166EXP0"/>
    <dbReference type="Gramene" id="KZN07102">
        <property type="protein sequence ID" value="KZN07102"/>
        <property type="gene ID" value="DCAR_007939"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209461"/>
    <dbReference type="OMA" id="HRRIMYN"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF42">
        <property type="entry name" value="SHAGGY-RELATED PROTEIN KINASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZN07102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG89738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="380" mass="42942" checksum="6B93D5C20124BE69" modified="2016-07-06" version="1">MADDKEMTAPVVDGSNEVTGHIISTTIGGKNGEPKQTVSYMAERVVGTGSFGVVFQAKCIETGETVAIKKVLQDRRYKNRELQLMRSMNHPNVVGLKHCFFSTTSNDELFLNLVMEYVPETMYRVLKHYSNANQQMPLIYVKLYTYQIFRGLAYMHAVPGVCHRDLKPQNVLVDPLTHQVKICDFGSAKMLVKGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVGQLVEIIKVLGTPTREEIRCMNPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCSPLEACSHPFFDELREPNARLPNGRPLPPLFDFKQELSGASPELVNRLIPDHVKRQMGLQFLHPTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FRU9</accession>
    <name>A0A166FRU9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FRU9"/>
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        <property type="protein sequence ID" value="KZN08102"/>
        <property type="gene ID" value="DCAR_000771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="285" mass="31237" checksum="5EE67B572D77E689" modified="2016-07-06" version="1">MFIFDTIRFQEEFQIPEELQEAMMLLNSLYANNFEEQKSARIVSWIPVEQNQSAHYLADYGLANVNLFGEVPRTLGNLQHFLDRDMGIAVPFDVMDNLGMGEVVDADPPPKPVHISSPHVDSAFPTLEHNTLLDLFHAFISQEGFKSKAQSMGSFVFPKHGSTLAVPTDTSSLKGKGKMYEKASFNGNGVLSSAAVEVLESGSLNHISEVFAQSSVNLDVQVFQDVSAKEILDQAVQGWSSRGNGASSSRVPGSARANSDMMEVADVMAEWDSFVAPNAEEQEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G4P9</accession>
    <name>A0A166G4P9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166G4P9"/>
    <dbReference type="Gramene" id="KZN08530">
        <property type="protein sequence ID" value="KZN08530"/>
        <property type="gene ID" value="DCAR_001060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="161" mass="18049" checksum="7017FF39B230A60E" modified="2016-07-06" version="1">MASGMNTIQTLPLDMIGELLEATLGHGFQCFNNLFKAWAQSQRTLVIRKLLGNLSISRLFRFWKRGSTEDTASFEEFMHVACDMGVGDAIVFTSCRELFSNPGNNNAQFAALEELSGRGYFLGQVATFITKVLFRRHSSVSALNALLSLHQNNLYRQIYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IJE6</accession>
    <name>A0A166IJE6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0104086</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IJE6"/>
    <dbReference type="Gramene" id="KZN11194">
        <property type="protein sequence ID" value="KZN11194"/>
        <property type="gene ID" value="DCAR_003850"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205028"/>
    <dbReference type="OMA" id="DFHDLCE"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000003">
        <property type="entry name" value="Obg-like ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="32"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="392" mass="44381" checksum="67666B4992F44469" modified="2016-07-06" version="1">MPPKSAKAAPVEKPILGRFSSHLKIGIVGMPNVGKSTLFNTLTKLSIPAENYPFCTIEPNEARVYIPDERFEWLCQMYKPKSEVAAFLEIHDIAGLVKGAHEGQGLGNNFLSHIRAVDGIFHVLRAFDDPEITHVDDTVDPVRDLETISQELRLKDLEFMARTIEDVEKSMKRSNDKQLKVEHEVCLKVKAWLDQGKDVRLGEWKAAEIEILNTFQLLTAKPVVYLVNMNERDYQRKKNKFLPKIHAWVQEHGGETMIPFSCVLERNLADMPADEAAKYCEEKQLQSALPKIIKTGFAAINLIYFFTAGPDEVKCWQIRRQSKAPQAAGTIHTDFERGFICAEVMKFDDLKELGSESAVKAAGKYRQEGKMYVVQDGDIIYFKFNVTSGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IUA6</accession>
    <name>A0A166IUA6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IUA6"/>
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        <property type="gene ID" value="DCAR_004153"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN11497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="173" mass="18764" checksum="8E041C5FF4927354" modified="2016-07-06" version="1">MADGFNLPGRKRNKENAADGSFSGIREYAGGRALDSFLQKRAENIKKSRTNPPGFAPVEGSLQNSSGSNRSPLSTVDHNSPLGFVMGTTTKGSERTPVGSTGPNNAAGHVNDTQERGNKKAFCFASYGCHCPVMNQYQYILNERITHSTTLLHFTHLSPIFSVNQLTGMTRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Gramene" id="KZN11649">
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        <property type="gene ID" value="DCAR_004305"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85438.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="379" mass="42803" checksum="CC91890FCEA6D8EF" modified="2016-07-06" version="1">MSLPQDLIDLVLFRLPVKPLLRCRCVCKGWCSLIDSNAFVKKHHKRMIECSTRGVIISGFDGKFYLTDFESLDGGGDEAVAVPMNGPVKSIVSGAEFFGAANGLVCLSKNQMNELFILNPSTRKARKIPSAPAYFPRSFDSSEIGLCGFGYDHVNDDYKIVKIGECHVKFRGILVIVYSLKTNSWKRIQNVPSKNTQFLGDRGLFANGALHWFTISGIMDFANIVSYDLGLEMFKEIPFPPHGTFGRSLVPVEESFGILYNYPSRVDVWLMNNSGEGNLWSKVLSLKQAGPLGPFSFVRPVAFSKCHKNVLLEVDRAKLVWYDIKRKRVKNVRIHGIPNSFESYLFIESLLQITNDKEDKLLQKPSQDNQGKKEQKKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J7Q1</accession>
    <name>A0A166J7Q1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_004537</name>
        <name evidence="5" type="ORF">DCAR_0104911</name>
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    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG85719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J7Q1"/>
    <dbReference type="STRING" id="79200.A0A166J7Q1"/>
    <dbReference type="Gramene" id="KZN11881">
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        <property type="gene ID" value="DCAR_004537"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1875580at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN11881.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85719.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="199" mass="22204" checksum="9845D74BE38BC127" modified="2016-07-06" version="1">MPKPALGPQRTSLIIWFAAIFCAVVAVAVVAIGIFVIVSYLVIHPTTPTISVSYANLDRFAYNQIGILDVQVSIVIKAKNENVKNHAVFYGLVLTLNFHGLPVAKLVNNPFDVKKNSSREFVYVVQSNQIPLRSVYSDYVQSALTKHKVSFELKGKVKTRWRVWVVGLVKFSVNMDCDLQFFVPNGSFTYRSPCSSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175Y933</accession>
    <name>A0A175Y933_DAUCS</name>
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        <recommendedName>
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    <organism evidence="17 18">
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 16">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175Y933"/>
    <dbReference type="Gramene" id="KZM80045">
        <property type="protein sequence ID" value="KZM80045"/>
        <property type="gene ID" value="DCAR_000462"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192295"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000027">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004970">
        <property type="entry name" value="PRK06333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG84993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="459" mass="48920" checksum="C53C70FD8E203C47" modified="2016-09-07" version="1">MQSLLHSTFTPAFRYRNPTLTSPKPPRKFSVFAASAAPRREKDPKKRVVITGLGVVSVFGNDVDTYYDKLLAGESGVDFIDRFDASEFPTRFAAQIRGFSSEGYIDSKNDRRLDNCLRYNIVAGKKALENAGLGEDMRSKIDKERAGVLVGSGMGGLTVFCDGVENLITKGYKKISPFFIPYTITNMGSALLAIDLGFMGPNYSIVAACATSNYAFYAAANHIRSGEADLMLAGGAEAAIVPVGLGGFTSCRAMSQRNDDPKAASRPWDVDRDGLVMGEGAGVLVMESLEHAMKRGAPIIAEYLGGAVTCDAHHMTDPRPDGVGVSMCIQSGLQNAGVSPEEVNYINAHATSTLAGDLAEANALKKVFKNNMSEIKVNATKSMIGHCLGASAGLEGVATVKAIATGWLHPTLNQVNLEPEVDFDTVPNKKQQHEVNVGISNSFGFGGHNSVVVFSAFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YG62</accession>
    <name>A0A175YG62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7054 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_030235</name>
        <name evidence="3" type="ORF">DCAR_0935118</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YG62"/>
    <dbReference type="STRING" id="79200.A0A175YG62"/>
    <dbReference type="Gramene" id="KZM82666">
        <property type="protein sequence ID" value="KZM82666"/>
        <property type="gene ID" value="DCAR_030235"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201879"/>
    <dbReference type="OMA" id="NQNANAR"/>
    <dbReference type="OrthoDB" id="1919859at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040358">
        <property type="entry name" value="At4g22758-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055482">
        <property type="entry name" value="DUF7054"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33270">
        <property type="entry name" value="BNAC05G50380D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33270:SF18">
        <property type="entry name" value="OS02G0324700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23156">
        <property type="entry name" value="DUF7054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7054" evidence="1">
        <location>
            <begin position="12"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM82666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="135" mass="14890" checksum="82E708C0AAF41BA1" modified="2016-09-07" version="1">MLSKQKKNQGDKRNRLLVSVTVMGSAGPIRFVVHEEELVANVIDQVLKCYAREGRLPVLGSKPDNFVLYSPIAGTEALSPADTIGSFGVRNFMLCKKPGLDKAIEQDAAPKMTRKSSGSWKTWFNKSLNLKISSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YHC8</accession>
    <name>A0A175YHC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial intermembrane space import and assembly protein 40 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0935233</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
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    </evidence>
    <sequence length="562" mass="62428" checksum="79349A9D7705A5AF" modified="2016-09-07" version="1">MEKDQELRFVCKWCDKKYPCGKSLGGHMRSHVIAAANSAESDEKLSALMAETGLIKCEAPSVAAVAGAGGGGINGDFGGPSGGYGLRENPKKTWRAVGSTFPIQQQERICKQCGKGFQSLKALCGHMACHSEKERVLKDDHSWTSEDPKLVMDGHSDTEEAEDLRRQTRSKSKRYKRIVVKSSHFNLVNNNSINYGSSSVSEVYEQEQEEVAICLMMLSRDSGNWGGVNSVVESSDNNSVILETKSSSIDMKLGRKGGLSLGHEALEMKKLGGARKLKLSALDAESVQLENSHSGYFKNGVKMVDSDISVDELFRYGEYRKSLKGHEGVEISNADMDMGFNRGKNYETGLSKSFGKEKGFSEFGSSSKSLKYDLKRTRDAPESPELRKESLRRLIYEDSSDGELSKNVYKRSKYECLNCNKTFNSHQALGGHRPCHKKPTAKFGSRYASGENNLDDDDNPPDMTPTRKYESVSNKKQYASNAAKKIRTKKNKGHECPICFRMFKSGQALGGHKRSHFINGAEDFSNQVPLFKQDAAEIHDMIDLNLPAPEEDEADENEKFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YI56</accession>
    <name>A0A175YI56_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0935755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YI56"/>
    <dbReference type="Gramene" id="KZM83209">
        <property type="protein sequence ID" value="KZM83209"/>
        <property type="gene ID" value="DCAR_030778"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200755"/>
    <dbReference type="OMA" id="QWRAGPF"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF42">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH16206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="27633" checksum="FE0564357ACCB559" modified="2016-09-07" version="1">MEALDEFLNFSDEDEKTKLPLSSSPLFSFNGLKDDDPPSVTEFVEEELEWLSNKDAFPALESCMDIILDQSAIFQSDQNHLSPVSVLENSGTSYIGNLMVPTSFPVRGRSKRTRKRRRRFPELSIRQPWQWNQMSMEDLKQEHENPFVSKTVTRVATIGRKCQHCQTEQTPLWRAGPMGPKTLCNACGVRYKSGRLVPEYRPASSPTFSSKLHSNSHKKIMEKRRQSQLSEMVVNGVCGYGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YN38</accession>
    <name>A0A175YN38_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_027559</name>
        <name evidence="8" type="ORF">DCAR_0831952</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YN38"/>
    <dbReference type="STRING" id="79200.A0A175YN38"/>
    <dbReference type="Gramene" id="KZM85019">
        <property type="protein sequence ID" value="KZM85019"/>
        <property type="gene ID" value="DCAR_027559"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199849"/>
    <dbReference type="OrthoDB" id="10261634at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="14"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM85019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="308" mass="33793" checksum="E4CF363BDFF4DB71" modified="2016-09-07" version="1">MLAITGNRFYTVGLISAWYSSNIGILLLNKYLLSDYGFKYPIFLTMCHMTACSLLSYVAVVRMELVPMQMLRSQNQFFKICVLSFVFCASVMSGNISLKYLPVSLNRAIGATTPFFTAVFAYLITMNTETLLVYATLVPVVIGVVIASGEEPSFHLFGFIMCVGATAARALKTVLQGVLLSSEGEKMNSMNLLLYMAPVAVVLLLPVTLYMEGNVVEITLALSRVDFRIVWYLLFNSVLAYFVNLANFLVTKHTSALTVQVLGNAKGAVAVVVSIFIFKNPLSVVGMFGHALTVLGVILYSEAKKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YP63</accession>
    <name>A0A175YP63_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0832492</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="9">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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    <feature type="domain" description="Cation/H(+) antiporter central" evidence="13">
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            <end position="615"/>
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            <begin position="621"/>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQX3</accession>
    <name>A0A175YQX3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_026705</name>
        <name evidence="4" type="ORF">DCAR_0832911</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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    </evidence>
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            <dbReference type="EMBL" id="WOH13401.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="8289" checksum="2733BA701F3996C4" modified="2016-09-07" version="1">MASAAATSSLVMKLLLVLVGLLFISHMVQLTSHETSFSPSLAARSRRHSPSPRERRERDCEDRTLICGSSSPPGGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W194"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434:SF16">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="6" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG80934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7565" checksum="3C038AE7BC00B075" modified="2024-05-29" version="1">MVRALMCLELILNAVNINFVTFSDFFDTIAAAEAAIGLAIVSSIYRNRKSTRINQSNLLNKNDTLGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W241</accession>
    <name>A0AAF0W241_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication protein A OB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0101078</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W241"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04480">
        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04481">
        <property type="entry name" value="RPA1_DBD_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="3">
        <location>
            <begin position="17"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="3">
        <location>
            <begin position="487"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="4">
        <location>
            <begin position="613"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="905"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="907"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG81922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="106977" checksum="1F7FEE229E561C56" modified="2024-05-29" version="1">MSEKTTSSFIVNDLSPYSPIDTVDTTTYDWKLRVRVQSFWKSMSREKQEFWGVNMLLIDDSNGRLHAFANSKYCGDLLKEIKEGEIYVISNFKVKDYLGDEKYRAVRSKKHIFFTPHTLFKKATDVGLPIELYAFDLFHYDALEKLADDNRSSVHVTFFDQFGELAEKDFRNADCRNLYVIICCAKVGRYEGLAHLSNYPATRVYINPKHYCMNELKRRCISEKKNEAVMVEIQEEEEVVVQMPRKILKVKDIKSLPKDFGEDSIFCEVTVKRITDNKSWYFRKCTGCDLELELEHGKFICSRPNGCGRIIPYPEKRFRLCTLCSDETGSVAIIFPDHEITKLIDKTVIDLHADCSDEAEEEKFPEVLKVFLKQKYTINLSINQENVEKGSTVFHAKEILQAQEKGDSFDPNSATVVEIGDISLVNESGTEQNASETPNTEKSTNMKTRPRNISESLAFNPTDASVSPLVKKIKLEKQFKTMASNHYTAVENLKPGVDQYKIKVRVIRLWRGATKSGEEFKSFNVIVIDQKGHRIHGFVPTKCADEFQFIVYVGRVYSIKNFDVQIYKQTEKFRVLRNATQLVFNQDTIIQQLADDGVTIPANAFDFYDHSQLEELSKQTTYLADVVGIIKDYDNIRDLTNRHGQSQRQAKFYITDGNSNISVTFWDNFGQNFDKLMKSGLEKPVIIIISGCRVGKWNGEIDISNNTATTVYLNYKHHSVVTLRKLLTNPEFAKKALGKPKVKSMAMATVKELENLGKDDIEGFFMAHVKITKIDETFSWFYNACTSCDMEVTVGNPCPICEACNRYVPYPQKKFKIHVVAEDQTGQMPVVLGDREVRAITGRRASDFADQIFSVQAFPGCVLGITDKEYSIVIHIREANVLNKFKYYWATNICRGFVKLPAEANEGASSSRAPTSQAAESTDNDQGISTVDLKSSLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W503</accession>
    <name>A0AAF0W503_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0102316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W503"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009768">
        <property type="entry name" value="MAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31246">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 70-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31246:SF5">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 70-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07058">
        <property type="entry name" value="MAP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="18"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="128"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG83142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="565" mass="64819" checksum="23C99BB6A86D8DE7" modified="2024-05-29" version="1">MVGLEELVAHPDPVILELNRLQNQLKEKERELGDTRSEIKGLRTADVLKAKAVEELANELNKLDEKLRLNEALLEQKNLDIKKLTNEKKEALAAQYAAEATVRRVHADQKDDDSFPIESVIAPLEADIKMYKHEVMTLQEEKRALERLTKSKETALLEVEKILKSALERALVVEEIQNQNYELKRQIEICQEENKILEKTNRQKVIEVQKLGETIQELEEAILVGGAAANTIRDYRRRISELNEENRTLERELARIKVSANRVATVVANEWKDENDKVMPVKQWLEERRVMQAEMQRLRDKLAISERRANTEAQLKDKLKLRLKTLEEGLKQTPNITVNQKASSGSPKPGNSSSTENVSNGERMRSVSQPRASAINRNLQKPILEKELKRGNSLKMRYGFGELLVRKGLWSSRSKVGNIDELERAKTGKENDVNIDKMKENGSEVLDNVEPYVAVREDSESITGLNPQTDDSVSGFLYDKLQKEVISLRKSCEKKDNNLSAKDEEIKVLMKKVDTLVKALEVESRKIKRETAEREKNSAANMINGYNNSRKTKRSDLVYRRFQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W748</accession>
    <name>A0AAF0W748_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mannan endo-1,4-beta-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0103669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W748"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057232">
        <property type="entry name" value="DUF7910"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010431">
        <property type="entry name" value="Fascin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551">
        <property type="entry name" value="FASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551:SF9">
        <property type="entry name" value="FASCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25490">
        <property type="entry name" value="DUF7910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7910" evidence="6">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="164"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG84485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="53938" checksum="0CF0414584A7EA15" modified="2024-05-29" version="1">MLDGTGVQFKSVTSQKYVSSENGGGMNISVDRDVPSLWETFTLWRISETKFQFRTFQGQFLTYDAEGNFVSATAQSPSATETFYLERNIDNRVHIRNNRGTYLQASSINQLTADYPGNPGWEDNAATFQMIIASNTLHGDYQLANGYGPDKAKEVLKTHRDGFVTEQDFDFLYRHGINTVRIPVGWWIASDPNPPAPFIGGTLDALDNAFSWAQAYNIKCIIDLHAAPGSQNGMEHSASRDGTTGWPTPSHISQTLNVIKFLASRYAEHPALLGIELLNEPSAWTVPLDILISYYQQGYQIVRKYSPTAYVIVCQRIGNADPIEIYQADIGSRNVVVDLHYYNLFDTNFINMSAADNIQFIHKSRETQLQALNSRNGPLIFIGEWVNEWDVVNSSNADYQDFGRTQLDVYNMASFGWSYWTLKNAEHHWDFEWNIRNNILQLGKYCRPIYCFIKVTETERNAHSHDSVTHTQGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W750</accession>
    <name>A0AAF0W750_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0103655</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W750"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056876">
        <property type="entry name" value="Msl2-3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057483">
        <property type="entry name" value="MSL2/3_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045042">
        <property type="entry name" value="YnaI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634:SF2">
        <property type="entry name" value="LOW CONDUCTANCE MECHANOSENSITIVE CHANNEL YNAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634">
        <property type="entry name" value="OW CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24956">
        <property type="entry name" value="Msl2-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25237">
        <property type="entry name" value="MSL2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive channel protein 2/3 transmembrane" evidence="12">
        <location>
            <begin position="139"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="10">
        <location>
            <begin position="270"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel protein 2/3 C-terminal" evidence="11">
        <location>
            <begin position="345"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="470"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24956"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG84472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="731" mass="80394" checksum="BE1A8B7F15E23B16" modified="2024-05-29" version="1">MGTGCTLQLSPQLGMYRNHPSSREFQVPNYLFSCLIKPSLICLSDRLHKRVHAAPYKRNHFTCHSLLNLGHSLHISSLKNTAAELTRSCGALQGKHVVFKLVPAITILLFAVWGLGPVMRLSRNIFLHKSDKSWNKSGTNYVVTSYLQPILLWTGATLICRALDPVVLPSEASELVKQRLLNFVRSLSTVLAFAYCISSVIQQTQKYFMETSEPADSRNVGFQFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIQATRPFIVSEWIKTKIEGYEVSGTVEHVGWWSPTIVRGDDREAIHIPNHKFTVNVVRNITQKSHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRVFLENVNPENQALMILISCFVKTSRFEEYLCVKETILLDLLRVISHHRARLATPIRTVQKMYGDADLDSMPFADSVFTRGRAPTNRPLLLIEPSNKVNGEGKTKSQTRSARTNGEQDNKAVARTSDNQIDVKGGPSDAKTKEKSSSDSKVKANSSAGVVPDPDSKKDQKFEGMPVYDTKQGDKVGDKVLSKSTAKAVSKIGEISSSNSNAPVSKNVTENNSTKKQPKNGELKSAPQNIVVENPSAAVPETSKRKANSSISNSKQAERLTAVDIPASRPVSEENIVLGVALDGSKRTLPIEEGMPPSATSKEVKELATSRSGSASSTVDKDKKDNQNSAGATPADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7I8</accession>
    <name>A0AAF0W7I8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043459">
        <property type="entry name" value="NFD6/NOXY2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156">
        <property type="entry name" value="OS02G0230000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156:SF37">
        <property type="entry name" value="PROTEIN NUCLEAR FUSION DEFECTIVE 6, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="102" mass="10966" checksum="D4BCBC79C35ABA34" modified="2024-05-29" version="1">MAAAAYRCGGSRLLSSSKRLFISKTLTPKSFPTPSASTSFTPLRYFYSSYVLYSTLLFASVLASVESLMPLHSAIASARLTSKIALHSSCWSCLSQGFATPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7M1</accession>
    <name>A0AAF0W7M1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="15">Guanine nucleotide-binding protein alpha subunit</fullName>
            <shortName evidence="15">GP-alpha</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0103955</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The helical domain is required for self-activation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the G-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7M1"/>
    <dbReference type="KEGG" id="dcr:108227928"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010476">
        <property type="term" value="P:gibberellin mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanine nucleotide-binding protein alpha-1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000733">
        <property type="entry name" value="Guanine nucleotide-binding protein alpha-1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002976">
        <property type="entry name" value="Plant_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01242">
        <property type="entry name" value="GPROTEINAPLT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 15" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="56"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="195"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="226"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="391" mass="45632" checksum="95E6C81D50D3403C" modified="2024-05-29" version="1">MLFSVLENMGSLCSKQRHNPADSEENAQTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIKLLFQTGFNEAELKSYITVIHANVYQIIKILYDGAKELAQNEEESSNYALSDEIKEIGEKLSEIGGRLDYPRLTKELAQEIETLWKDDAIQETYSRGNELQVPDCTLYFMENLQRLAVADYIPTKEDVLYARIRTSGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAVSEYDQTLFEDENRNRMMETRELFEWVLKQPCFEKTSFMLFLNKFDIFEKKVLDVPLNVCDWFKDYQPVYSGKQEVEHAYEFVKKKFEELYFQSTAPHCVDRVFKIYRTTALDQKLVKKTFKLVDETLRRRNLFEAGLL</sequence>
</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="155" mass="17203" checksum="0B7A8623EF4A2560" modified="2024-05-29" version="1">MKKCELCNSIARMFCESDQASLCWQCDAKVHSANFLVAKHSRTLLCQSCQALTPWTGSGPKLGPTLSFCESCVHENTNYNNDTDEDDEDEEDDEDDEEDEEEDDGENQVVPLSSSSLAPPQVTSSSSSEESSARLFNGAESSRKSRFSISWSRTR</sequence>
</entry>
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                <person name="Senalik D."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="6">Leaf</tissue>
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    </dbReference>
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    </dbReference>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFY8</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFY8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="233"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG88827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="477" mass="54556" checksum="9F2626244461418A" modified="2024-05-29" version="1">MFALAIIYQYSPKFRPLVKSLMFVANMLQQSSPYLQQALNRYIQKIESSCDPYIQITFHEHSSRQFSERSGAYVAIQRYLEANSSRMGKRLKAHSVKGHESLVLSMNNNEEITDEYQGVRVWWTYSNNVVSTTNKLQLEKQRSYTLIFHNRDRDLITKSYMKHVLTKGKALSLKNRRRKLYTNKYGRWTEVDFEHPATFDTLALKADTKKDIVDDLITFSKAKDYYMKVGKPWKRGYLLYGPPGTGKSTLIAAMANLLHYDVYDLELTSVTCNTELKKLLTETCGKSITVIEDIDCSLDFTGKRELQNIEMKEKDKPSGNQKPSQVTLSGLLNSIDGLWSSSAGERIIVFTTNSLEDLDPALIRRGRMDKHIEMSYCSFEAFKVLARNYLEIESHKLFGRIEELLAETNISPADVAETLMLKSGDGENCLKCLISVLEDVKDISEDIKEEKHKVVKVDKRKKKVKEALAVKDVAANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHD7</accession>
    <name>A0AAF0WHD7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0207633</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHD7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001533">
        <property type="entry name" value="ABC transporter G family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF19">
        <property type="entry name" value="OS09G0472100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="123"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG88398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="722" mass="80736" checksum="88D5745BB7299F1C" modified="2024-05-29" version="1">MTAEKDAQEETIAPDLHQQPPYPSTNFPFPHAPCPNCVPPLQPEPPLIANASRHEAASLRLYDEKRSWKNRSSRVSDIERVQSAPRDDGARIYITWNDVWVTVPSKKGGRRPILQGLTGFVEPGQSLAIMGPSGCGKSTLLDTLAGRLGSNTKQSGDILVNGRKQTLAFGTSAYVTQDDSLMTTLTVREAVYFSAQLQLPNSMPIAEKRERAETTITDMGLQNAMNTRIGGWTVKGLSGGQKRRVSICIEILTRPSLLFLDEPTSGLDSAASYHVMSRIIRLAQQDKRTIIASIHQPSAEVFELFHNLCLLSSGRQVYFGSTGAANEFFTSNGFPCPTMRNPSDHFLRTINKDFDTEEEQKINGGQTAAEAIDILVKAFQSSEQRNQVQNRVSEICQMNVQTVASKRSQASFVTQCLVLTRRSFLNMHRDLGYYWLRLIIYTALAVCIGTLFHDIGHTYGSIQARGSMLMFIAAFLTFMAIGGFPSFVEDMKIFTRERLNGHYGVAAFVVANTFSSVPFLFLIGLIPGAISYYLVGLQKEFGHFVYYALLLFVCMMLVESLMMVVASVVPDFLMGIIAGAGIQGIMMLTGGFYRLPDDMPKPVWKYPMYYISFHKYAFQGFYKNEYIGLTFPNEKAGGPPTITGDEILRDIWQVEKGYSKWVDMTILLAMVATYRVLFFLITKGVEKFKPVIRDFLSNISFKQQSDSEDSLHQQESSVPFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ28</accession>
    <name>A0AAF0WJ28_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0208520</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ABC-type transporter activity"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="term" value="P:hormone transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of secondary shoot formation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ABCG_PDR_domain2"/>
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        <property type="entry name" value="ABC transporter G family member 34"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_trans_N"/>
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        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
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        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF14510">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="571"/>
            <end position="598"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="619"/>
            <end position="644"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="656"/>
            <end position="675"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="682"/>
            <end position="705"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="711"/>
            <end position="730"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="764"/>
            <end position="791"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="1185"/>
            <end position="1203"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1322"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1328"/>
            <end position="1347"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="1359"/>
            <end position="1378"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="1408"/>
            <end position="1430"/>
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            <begin position="169"/>
            <end position="442"/>
        </location>
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            <begin position="839"/>
            <end position="1091"/>
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            <begin position="1"/>
            <end position="27"/>
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            <begin position="1"/>
            <end position="19"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="1438" mass="161238" checksum="061074BEC9218997" modified="2024-05-29" version="1">MDGSNLYNLSNSVRNSRNSRSTRERAGSLRSSSSAIWRSSGMEVFSKSARDEDDEEALKWASLEKLPTFDRLRKGLLLGSQGAGASEIDIDNLGTEERKKILERLVNVAEEDNEKFLLKLRDRVDRVGIDLPLIEVRFENLKIDAEAHVGSRALPSFLNFNISIVEGLLDTLHLLPNRKQRLTILDDVSGILRPCRMTLLLGPPSSGKTTLLLALAGKLDPTLKFSGRVTYNGHEMTEFVPKRTAAYISQHDLHIGEMTVRETLAFSARCQGVGSRYEMLAELSRREKDANIKPDPDVDIYMKAAATEGQEASVVTDYVLKVLGLDVCADTMVGDQMIRGISGGQRKRVTTGEMLVGPSKALFMDEISTGLDSSTTYQIVKALRHSVHILQGTALISLLQPAPETYDLFDDIILISDGQIVYQGPRENVLEFFESMGFKCPERKGVADFLQEVTSKKDQKQYWLRKDEPYRFITAIEFSEAYQSFHVGRRLGEDLATPYDKNKSHPAALTTDKYGVGKTELLKAVTAREILLMKRNSFVYVFKVFQLTTMAIITMTLFLRTELHQNSVTDGGIYMGALFFGVVMLMFNGLAELAMTIAKLPVFYKQRDLLFYPTWSYSLPVWIIKIPITFIEVAVWVVLTYYVIGFDPNVGRFVKQYIVLLLINQMASALFRLMAALGRNMILAFTFGGFALLVLFALGGFVLARDDVAKWWLWGYYSSPMMYGMNAIAVNEFLGHQWHKLVEDGSETIGVAILKSRGFFPYAYWYWIGVGALIGFVLLLNFAVAIALTYLDPLGKPQAVIPDKDSAEAIELASRGGTSAEANENKKKGMILPFEPHSITFDEVMYSVDMPPEMKEQGVVEDKLLLLKGVSGAFRPGVLTALMGVSGAGKTTLMDVLAGRKTGGYIEGNITISGFPKKQETFARIAGYCEQNDIHSPHVTVYESLIYSAWLRLPSEVDSTTRKMFVDEVLELVELNTMKAGLVGLPGVNGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIHQPSIDIFEAFDELFLMKRGGQEIYVGPVGRHSCELIKYFEGINGVNKIKDGYNPATWMLEVTSSAQEMVLGVDFTDVYHNSDLYRRNKALIQENSSPRPGAKDLYFPSQYSQSFITQCMACLWKQRCSYWRNTSYTAVRFLFATAIAVMLGSMFWDLGSKLRSRQDLFNAMGSMYAACLFLGVQNASSVQPVVDVERTVFYRERAAGMYSALPYAFAQVLVEIPYVLAQAGVYSIIVYAMIGFDWTVVKFFWYVFFTFFTLLYFTLFGMMTVAVTPNADIAAIIAAAFFALWNVFSGFIIPRPKMPVWWRWYSWVCPVAWTLYGLIASQFGDRDDKTLSDVKVTVKKFVEDYFGFEHHNVWVAGAAVAGFTVLFAYTFAYSIKSFNFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLL9</accession>
    <name>A0AAF0WLL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0209732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLL9"/>
    <dbReference type="KEGG" id="dcr:108205651"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000024">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="184" mass="20983" checksum="1B6334D55E7F0E77" modified="2024-05-29" version="1">MSSPSKRREMDLMKLMMSDYKVEMMNDGMQEFYVHFHGPSDSPYQGGVWRIRVELPDAYPYKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALMMRDRTSYEQRVKEYCEKYAKAEDVGEAPEESSSDDEMSEAEYDSSDEVMAAGPVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMD1</accession>
    <name>A0AAF0WMD1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0310140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMD1"/>
    <dbReference type="KEGG" id="dcr:108214059"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF4">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="667" mass="73018" checksum="B13D11FCD6741CC5" modified="2024-05-29" version="1">MGSNEMEISREIENDQLGEVRNEVEDVKRLPPWTTHITIRGIVASIAIGIVYSVIVMKLNLTTGLVPNLNVSAALLAFVLIRTWTKLIHKAGYVSSPFTRQENTIIQTCAVACYSIAVGGGFGSYLLGLNKNTYEQAGIDTEGNSPGSYKEPAVGWMTGFLFVSSFVGLLALVPLRKIMVIDYKLSYPSGTATAVLINGFHTPKGDQMARKQVHGFTKFFSISFLWGFFQWFYTGGTGCGFVNFPTFGLKAWRQTFYFDFSMTYIGAGMICSHLVNLSLLLGAVLSWGLMWPLIGEQKGVWFPETLPESSMKSLNGYKVFISIALILGDGLYNFLKILYFTSKSICTSMNKKNNKITSDDGNHPIDELQRNEVFIRESIPLWIACSGYVLLSVVSIIVIPIMFPQLKWYFVLVAYVLAPSLSFCNAYGAGLTDMNMAYNYGKVALFILAALSGKDNGVVAGLVGCGLIKSIVSVSSDLMHDFKTGHLTLTSPRSMLVSQAIGTAIGCVVAPLVFFLFYKAFDVGNPDGDYKAPYAIIYRNMAILGVEGFSALPQHCLQLCYGFFSFAILANLLRDISPKKVSKWVPLPMAMAVPFLVGAYFAIDMCMGSLVVFVWHKLNSNKASLMVPAVASGLICGDGLWILPSSILALAKINPPICMNFLATKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMX7</accession>
    <name>A0AAF0WMX7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310142</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMX7"/>
    <dbReference type="KEGG" id="dcr:108213968"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1193">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPP13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="173"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="420"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="544"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="874" mass="100093" checksum="1B013827102545E7" modified="2024-05-29" version="1">MVDAAVSFAIEKLHEFVSQQVNIRIGVKDGIEWLKDELGYLLISVRAAEAHQDKDHIRRWTDSVKDVANQAVIILERFSAQQEQHAADEQGGVLECMRRLICICKKEANLYDIGKDIESLKEKIIGIKSRRDEYHINDIIIATPRVQKRRRTLLRAASFDHEKDVVGFEVDYQTLLAQLDNQDSSLGFISIHGMGGLGKSTLASKLYHSSELSHFKSRAWVCVSEDYDITHVLRKIIKCFEKDEQDLLNKMEEVELLRHLRKILLEGDHYLVVIDDIWDVDFWKKIKSAFPDKKNGSRVIITTRNKLVAEGVEDTCFIHELSFLSKDESWKLFCKRAKPTPNLEMLGKEMVDKCGGLPLAIVILSGLLLQKKTYQFWSNVKDQLWRKLKGESSEIQELLNLSYDDLSFNMRQCFLYLARYPEDHTIQVSKLKLLWIAEEFVEKRDGEYMEDVAEDYVNELINRNMIQIELQTVDGQVLVFKIHDLVRDLVIEKAREQKILGIFDPSQQHPDPIHLLQGQARHAIYNGIGEYLKLLGPNSDDLNLRSLAVTSEIDKIKVEEVKLMYTRFHYLKVLDLTSVHGSEGIPEAVGDLVLLKFLGLMGGDKRKDIVIPSSIGKLKSLQTLHGLDWPDNEFEFPKEISELKELKHIIFHGNEGNLNIGRDQTKLQTISTIMYKEWCHIDTNKWTNLHTLVISEEEDSYEEEEYSLESIANLTSLRTLVLLLYGVISTMQPLSSCKHLNKLVLLCKIKDLAEVNFLPDSVTDLTLSVTASTEDPMPILGSLSNLTSLWLMNSGDKMVCSADAFPCLQFLRIQPATGALFELQVDDGALPSLRAFTLERLPFLRAFLLNADRDMIPRRLKSLPDVPDFQQYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WR82</accession>
    <name>A0AAF0WR82_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0313938</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WR82"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010328">
        <property type="term" value="F:auxin influx transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000044">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="711"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="755"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="848"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="929"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="407"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="1049"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG94642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1270" mass="136474" checksum="2D817936C4A2E5AF" modified="2024-05-29" version="1">MANDVSEDMSGTSTAQVLDTGVERSSGGHGSKEKESTNIVPFHKLFAFADSVDVMLMIFGIIGSIGNGLSMPLMTVLYGELANSFGQNQDRVHVIQVVSKVSLKFVYLGLGTGVASFLQVACWMVTGERQASRIRKLYLQNILRQDITFFDMETNTGEVVGRMSGDTVLIQDAMGEKVGKFIQMITGFVGGYFVAFFNGWLLTVVLMSSIPPIVMAGGMMSLVSSKMSTRGQDAYAKAATIVEQTIGSIRTVASFTGERQAVVNYNKSLTKAYKSGVQEGFASGLGLGTVMSVAFCSYAMAVWFGAKLVIEKGYSGGTVIVVIVAVLTGAMSLGQASPCMSAFAAGRAAAFKMFETINRRPVIDPYDKRGKKLDDIQGDIELRDVHFSYPARPDEQIFSGFSLAIPSGTTAALVGQSGSGKSTVISLIERFYDPQGGEVLIDGTNLKEFQLKWIREKIGLVSQEPVLFSSSIKDNIAYGKDGATVEEIRAACELANAAKFIDKLPQGLDTMVGEHGTQLSGGQKQRVAIARAILKDPRILLLDEATSALDAESERIVQEALDRIMVNRTTVIVAHRLSTVINADMIAVIHQGKMVEKGTHVELLENSEGPYSQLIKLQEVSNSRDKTDAGAGSGSASSQRMYSSISRVSSGVGNSSSRRSLTLSFRLPTGHTEIVIGEPEPKPEPEPKDPNQKTSPEVPLRRLAYLNKPEIPALFAGSLAAIVNGVTYPIFGVLLSSTIKILFEAPHELSKDSKFWALMFVALGTVSFVAYPAQSYFFAVAGCNLIKRIRSLCFEKVASMEVGWFDKPENSSGAIGARLSTDAATVRALVGDRLGQLVQDGASAVSGLIIAFVACWQLAFVILALIPLVAVNGYAMYEDASQVATDAVGSIRTVASFCAEEKVIDLYTKKCEGPKKNGIRQGMISGTGFGLSSASLYFVYAVSFYAGARFVALGITDFENVYRVFFALTMAAISISQSSSVASDSYKAKAAAASIFSILDRKPEIDLNEESGVTLQNVNGDIQLQHISFMYPTRPDVQILRDLSLTIRSGKTVALVGESGSGKSTVIALLERFYDPNAGNITLDGIEIRTLQVKWLRQQMGLVSQEPALFNDTIRSNIAYGKGGDATEAEVIAAAEKANAHQFISGLQQGYDTLVGERGIQLSGGQKQRVAIARAIVKSPKILLLDEATSALDAESERVVQDALDQVMLNRTTVVVAHRLSTIKGADVIAVVKNGVVVEKGKHEKLISITDGFYASLVALHTSNTREEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYN6</accession>
    <name>A0AAF0WYN6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0416518</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYN6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000018">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG97178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="408" mass="47454" checksum="B6CAADF70C5E4201" modified="2024-05-29" version="1">MSKGYHSVHVLLLSDCFPIPNLFTCKELVVRVGNAWLYEPNLNTLRQKVNLPVGSCELAVPLKSKDHYSSNARREAYATILHSAHVYVCGAITAAQSIRMSGSTRDLVILVDDTISEYHRGGLEAAGWKLHTIERIRNPKAERDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNIDILFEMPEITAIGNNATLFNSGVMVIEPSNCTFQLLMDHINEIVSYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWEGDEEEKKEMKIKLFGADPPVLYVIHYLGLKPWLCFRDYDCNWNVNILQEFASDVAHRRWWKVHDAMPENLHKYCLLRSKQKAALEWDRMQAEKGNYTDGHWKIQIKDSRLQACFEEFCFWESMLWHWGETNWTDNATATLAPPVPIISGLLPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0M9</accession>
    <name>A0AAF0X0M9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0518552</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X0M9"/>
    <dbReference type="KEGG" id="dcr:108223737"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG99204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="607" mass="67703" checksum="896EF028534A9EB3" modified="2024-05-29" version="1">MEMSSDQKEQQQQQEVPKKPKGGLITMPFIIANEAFERVASYGLLPNMILYFIMDYGMSLTKGQNILYLWNAATNFTPLFGAFLSDSYLGRFATIALGSVSSLLGMILLWLTAMIPEARPGPCDPRTPQLCKPASSFQHFLLYASFGLISIGAGGIRPCSLAFGADQLDKRDNPKNERVLERFFNWYYASASLSVVIALTGIVYIQDHAGWKVGFGVPAILMFLSASLFLIASSLYIKQTVRMNLFTSFCQVITVSYKNRKLALPPQDSDGWYHHQKDSIHYVPTDKLRFLNKACIIRRPEDITSDGVASNPWTLCTVDQVEEMKILIRVLPLWSSGIMMSINISQSSFQLLQAKSMKRHLIGGFEVPAGSFGLFTVISLFVWIVIYDRLVLPLASKIRGKPVYLGVKVRMGIGLFFSLLGMVASAIIEHVRRRKAIEQGLLNNPMGLVNMSAYWLVIQHVFNGLAEAFNAIGQTEFYYSEFPKSMSSIASCMFGVGMGVANLLASLILSTVDDITKRGGKESWTSTNINKGHYESYYWLLAIMSSVNLLYFFICSWAYGPCVERLNGIGIETSNGTGKELMKQSSRVKDETAEKSEELFKSRLLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X176</accession>
    <name>A0AAF0X176_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0518577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X176"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF87">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="90"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG99229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="186" mass="20670" checksum="CCCE544727CE85F5" modified="2024-05-29" version="1">MGTPGLGLEITELRLGLPCMGGKSKAAEKKRAFWEIDGEEDGSSSTTTEEKTQKVESGGKGSGNQVVVGWPPVCSYRRKNSFNGREKGSKTYVKVSMDGAPILRKIDLGPFGEYSELTRALQKLFHSFDHSEKLKDAENNEYVTIYEDKDGDWMLVGDVPWEMFTESCKRLRIMKNADAKGIGLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1H0</accession>
    <name>A0AAF0X1H0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transport inhibitor response 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0418232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with auxin and auxin-responsive proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X1H0"/>
    <dbReference type="KEGG" id="dcr:108218844"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000029">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000028">
        <property type="entry name" value="Transport inhibitor response 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF36">
        <property type="entry name" value="TRANSPORT INHIBITOR RESPONSE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="COI1 F-box" evidence="7">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="8">
        <location>
            <begin position="101"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG98886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="68372" checksum="5B79FA088B04CFC2" modified="2024-05-29" version="1">MSEAQAQMSEDDADLAAAAGGASIRTAVVTTGGDYSSPYPDQVLENVLENVLIFLTSRRDRNAVSLVCKSWYRAEALTRSEIYIGNCYSVSPRRAAERFRRVRSVVLKGKPRFADFSLMPPDWGAHFAPWVIEMAEAYRGLEKLYLKRMNVTDDDLAVMARSFGNFRELVLVCCEGFGTAGLAVVASKCRQLRVFDLIEAEVSDDDEVDWLSCFPESGTCLESLIFDCVEFPINFDALENLVARSRSLKKLRVNRFVNLEQLHRLMILAPQLTDLGTGSFSPQDHPIDQEPDLVSAFTACRSLVSLSGFRETVPDYLPAVYPVCANITSLNFSYANITADELKPVIRNCHKLQIFWVLDSVRDEGLQAVAATCKDLRELRVFPIDATEDNVGPVSDVGLQAISEGCKKLESILYFCQQMTNAAVISMSNNCPDLVVFRLCIIGRYRPDHSTGEPMDEGFGAIVKNCKKLTRLAVSGLLTDKAFSYIGQYGKLVRTLSFAFAGDSDLGLKYVLEGCSKLEKLEIRDCPFGDSALRSGLHHYHNMRFLWMSTCRVTRQGCREIAQQIPRVIVEVFRWNQILEEGEKSDLVDTLYLYRSLEGPRADAPKFVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X436</accession>
    <name>A0AAF0X436_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0520805</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X436"/>
    <dbReference type="KEGG" id="dcr:108224105"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000123">
        <property type="entry name" value="Putative methyltransferase PMT15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1049">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH01421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="637" mass="72114" checksum="CCFA29CDEFE8C5A1" modified="2024-05-29" version="1">MAGPVVHPEDYYTPTTKPNRPVPFWKKPQVYTIVVILCITSYFLGAWQHNGAATTTTTTSNLEATNIQCNPVVETTIKSPALDFTAHHKAEDVPVSKEVTKTYPACDIKLSEYTPCQDQKRSLKFSRDRLIYRERHCPEKRELLKCRVPAPFGYRAPFKWPVSRDLAWYANVPHKELTVEKAIQNWIRFEGERFRFPGGGTMFPNGADAYIDDIGKLINLKDGSIRTAIDTGCGVASWGAYLMSRDILPLSFAPRDTHEAQVQFALERGVPALIGVMSSTRLPYPSRAFDIAHCSRCLIPWGQNDGAYLIEVDRVLRPGGYWILSGPPINWQKHWKGWERTKEDLNAEQTGIENVAKSLCWNKFIEKGDIAIWQKPLNHMECKLNKKISQNPPFCPAQNPDEAWYTSIETCLVPLPEASDDEVAGGGLKKWPARLNAIPPRISQGTLKGVTAEVFQNDGELWKKRVAYYKNVNSQLGQAGRYRNLLDMNAHLGGFAAALIKEPLWVMNVVPVEANVNTLGVVFERGLIGTYQSWCEAMSTYPRTYDLLHADSVFTLYKDRCEMEDIVLEMDRILRPEGSIIVRDDVDILVKVKAIADGMDWESRIVDHEDGPLEREKLLFAVKRYWTAPAVPDEQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X635</accession>
    <name>A0AAF0X635_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0521875</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X635"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF90">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="440"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="4">
        <location>
            <begin position="605"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="841" mass="96179" checksum="11ABBEE9F06F6340" modified="2024-05-29" version="1">MTPHLGGRLFQQYIVDAFSTIEQARLWYIRTHQTTLRSDLYSQLCDSLRSGNTDASRVGKGIILPAGFVGSRRYMQQNFQDALAVCRYIGHPDIFLTMTTNPLWDEINEMMKLLPHCLPQNSPDIIARVFKLKLDQLVDEIKNKEFFGKCIGVMYVVEFQKRGLPHVHMLIWLSSDSKKYLQANICCHTRSIKYIFKYCLKGHDRATVEIKSRKKDTAESSKDSPVDEINQYFDGRYICACEAAYRIFGFNIHYRTHSVQRLSFHLPGERSCVQIGRLFYTNHSSGELWYLRLLLTKVRGPTSFKDLRTVNGKLCQTFQEACKEYGLLDDDNEWHQVLQQCSSCGLAPQIRELFVHIMVNCRVTDLKKLWNDHSVHMTDDIVLIRRKLPKSIGKSLNDFNDMPQPPRNYLDCGLNNLIIEETSYNLDLMAEEHKSLISEMNEEQLQVYNAIMDSLQKTDGGFFFVYGSGGCGKTYLWKTLISKLRSESLIVLPVASSGIAATLLPGGRTAHSRFKIPIMLDDCSTCNIAQQSDIAQLLHQHYNDPKYLSERAILTPTNNTVAHVNALIVDRIPGESQSYYSVDNAEDYPGTETELNNSFPPEFLNSLNVPVGVVVMLMRNLNQTLGLCNGTRMMVTKLLPQCVQCEVISGSFLGTRHFIPRMELFPTDSKLPYKLMRKQMPLQICYAMTINKSQGQSLEKVGLYLPKPVFSHGQLYVAISRVTSPEGLRIFVESQMGLTTNITANVVYKEFYKGDETYRAVRSGKHIYFNTDTLCSKVVDTCLKIQPLSFDLYCLDDVYALKKDNRFLIGEFLDSYISMQSVTYPLKLIMEKKSNLLLFIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X6F7</accession>
    <name>A0AAF0X6F7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0520034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X6F7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000008">
        <property type="entry name" value="BTB/POZ domain-containing protein POB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045890">
        <property type="entry name" value="POB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46336:SF3">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN POB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46336">
        <property type="entry name" value="OS02G0260700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BACK" evidence="4">
        <location>
            <begin position="49"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH00661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="25322" checksum="997E60225E2B31F3" modified="2024-05-29" version="1">MVKKFIDINNFLKLSLAWQRVQVLESYNPTNPLDSTGYLEHWIKPLVNCVLMAEAVQPLTDAAKQFLAVRYRDISKFTDEVMRLPLASIEAIFSCDDLQVASEDFVYHLILKWSRPHYPELEERQEILVSRLSRFIRFPYMSCGKLKKVLTCTDFDHEECFFLSAHCNLDQQSYFHCFGLFLGIKAVGYRNLFGIPWTEFISEDSQYILHQWCTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDK1</accession>
    <name>A0AAF0XDK1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0625266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
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    </reference>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </reference>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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</entry>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
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            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="2">Belongs to the CYSTM1 family.</text>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH05394.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="65" mass="7146" checksum="7850FB175148350B" modified="2024-05-29" version="1">MNEPPKYAYPYPAQGNYYGPPVMAPPQYAAAPPPRRESGFLEGCLAALCCCCLFEECCDVSIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEY2</accession>
    <name>A0AAF0XEY2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0625180</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEY2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019199">
        <property type="term" value="F:transmembrane receptor protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000468">
        <property type="entry name" value="LysM receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000468">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044812">
        <property type="entry name" value="CERK1/LYK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057097">
        <property type="entry name" value="LysM_RLK3/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204">
        <property type="entry name" value="CHITIN ELICITOR RECEPTOR KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204:SF15">
        <property type="entry name" value="LYSM DOMAIN RECEPTOR-LIKE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23577">
        <property type="entry name" value="LysM_RLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041988842" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="22"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="302"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH05759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="601" mass="66226" checksum="CAF3D8971975B67D" modified="2024-05-29" version="1">MFSVSCFGLLILSSVFVSVHSECSRGCGLALGSYYVWPESNLTIISDFTNIRIEEIVSYNKQLIPNQNSVQSDIRINIPFPCDCINGEFLGHVFKYSLRSGDTYDRIANLYYSNLTTVEWLKRFNTFDPNNIPDTNEKLNVTVSCSCGSDAVSKEYGLFVTFPLRVGDSLVSVANQSNLNLDLGAGSTLLQSYNPGVNFSAGRGIVYVPGRDKTGRFPPIKNSGGIAGISVAAIAGLLLFAGCFYVVYYRKRRVAKIELSPAAEDQSIQDSTVAASPRLTGITVDKSVEFSYEELAKATNDFNISFKIGQGGFGAVYYAELRGEKAAIKKMDMQASREFLAELKVLTHVHHLNLVRLIGYCVEGSLFLVYEYIENGNLSEHLRGSGDRPPLSWPVRVQIALDSARGLEYIHEHTVPVYIHRDIKSANILIDKNFHGKVADFGLTKLTEVGSTSMPTRLVGTFGYMPPEYAQYGDVSPKVDVYAFGVVLYELISAKDAIVKANGSMTESKGLVALFEEVLNQPTPKDDLTKLVDPRLGDSYLVDSVRKMAQLAKACTQENPQLRPSMRSIVVALMTLSSSTEDWDIGSFYENHALVNLMSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH81</accession>
    <name>A0AAF0XH81_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0626521</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XH81"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
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        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
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        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="828"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH07092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="100139" checksum="1C626569957AEB1D" modified="2024-05-29" version="1">MLTLRSMNRIRVFISPLPCIDLSYANYRFSSFLSPLGSDDNVHQLRAHDVVLAFKEWFRSRERYVRPSMDRIYVILKNTDDAVVIDSELMKLDLRLNERFVLDVLSCGEDVLSCLKFFDWAGRQRGFEHTRATFYAIFKILSKAKLMTLMTDFLDSYRRQSGVCKTRFYNTLVMGYAVAGKPDVALRLFAKMRFHGMDLDEYCYHVLLNALAEQGCFDAFDMVAKQIVFRGYQNNVTHAVLVKGLCKKKEWDRAESYTRDLVTSGTGLSEAVIGTLVAGLCRDKMFERAAEVVEEFGGSKVPLQAAYNMWIRELVRAGELEGALEILKKMKGMDGPDVFRYNMLINKLLKKNRLVEVYDLLMEMMEKQLCPNEETMNSVLCLFCKAGMVDDAVHLYDSKVEFGLSTSNTTHNFLISTLCAEGNTNEAYRVLRNSLTQGYLPGQNSFSIVSDALCREGKVDEMKDLVLLALERNFAPSSSIYNKVISAMSKNNRVEDGYMLHGQLNQLNKVTSRHAYMSLIDAFKKMNRGDIAVRLLIEMQEKGHDPTPTTFKHVIELLFDMENPEQKFLRMLEIQLSRQGQNRHVYRMFIFAAGHARKPDLARAVYDMMSASNIPPDVKTNTHMLLSYLKNERTASAVKLFNELKVEGKAGKKLGQCLIIGLCRMNKVEMALSYVEEMRRNKETPSLRCYEELVLVLCNNSKYDEVVNIVDDTIRDGRPLSTFIGNLLLLHSFKAENLYYYWIRSRHNTTSFSWALGGLIRAFSPYISDVPDIEDLEEVTQQLFPLDTFTYNLLIRRVIMAKRIPSGRRMENACTLFRRLRQKGLEPNRMTYDCIVHGFAKFGMESEARRWSEEMMRRGFEHTSCTKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNJ6</accession>
    <name>A0AAF0XNJ6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0729799</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNJ6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF75">
        <property type="entry name" value="PROTEIN RADIALIS-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH10332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="81" mass="9228" checksum="02C325B96CB8C6D6" modified="2024-05-29" version="1">MSLDLLSSSCSKSNWTIEQNKLFENALAMYDKDTPDRWMKIANATGGRSVEEIKMQYQKLVHDIALIEAGKVPLPNHLKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR46</accession>
    <name>A0AAF0XR46_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0830925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XR46"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000170">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF53">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="44"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="288" mass="31906" checksum="A342DDBB49493B5F" modified="2024-05-29" version="1">MAQSSFTSTDLGANPMQQQKKIVIQNEHGENLVGLLHETGSLEIVVMCHGFRSNKEFNVLVNLAAALEKEGISVFRFDFAGNGESEGSFEYGNYRREAEDLRAVVHHFSGMNHLVTAVLGHSKGGNVVLLYASKYHDVHTVVNLSGRFNLQNGLQTRLGKDFLERIKKDGFIDGKTKPGEANYRITEASLMDRLNTNMKEACVQIDKDCRVLTVHGSADEVVPVEDALSFGNTIPNHKIHIVEGADHCYTSHQDELAPVVLPFIKDGLQKAYNPNNLEIVVYVIKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRA4</accession>
    <name>A0AAF0XRA4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine carboxypeptidase-like 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0832195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRA4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000211">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF349">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041912433" description="Serine carboxypeptidase-like 45" evidence="2">
        <location>
            <begin position="26"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH12688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="52715" checksum="697731390C1D6BBE" modified="2024-05-29" version="1">MAVTNALRFLGAFVLLLSHVLWCGAEPITELPGQPENVAFQQHSGYIVTDALHGRALFYYFVEADTSDHLSRPLTLWLNGGPGCSSLGYGAFMENGPFQPGKKGNLIKNKYSWNLESNMLYLESPIGVGFSYSNTSSDYIMWNDTTTAKDNLAFIINWLEEFPMYKDSDFFLLAALILEHNKGKNTQPINLKAIGLGNPLLDIEISVEAGEFLWSHGAISDETLYLEKTVCNNSKFLQEYIHSGWSEGCNDVFNRVSDEMGADVLSDDLLLPICLSSSTSTTQFKPMGLHGKSHEAFARRGIVGDPCLQSRIFTYLNKPEVQKALHANTTHLPFHWDFCEGPLVYQEENLALNIIPLVRYLLNKGIPILLYSGDQDSKIPLTQTRIIADKLAKDLKLQSYTNYGPWYDKKQVGGWWQSYGRLTEGKNVTYLTFATVRGAAHEVPFTSPSQALTLFHSFLHALPLPTPPPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYI9</accession>
    <name>A0AAF0XYI9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0934912</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YABBY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYI9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010582">
        <property type="term" value="P:floral meristem determinacy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048479">
        <property type="term" value="P:style development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006780">
        <property type="entry name" value="YABBY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056775">
        <property type="entry name" value="YABBY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056776">
        <property type="entry name" value="YABBY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675:SF1">
        <property type="entry name" value="PROTEIN CRABS CLAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675">
        <property type="entry name" value="PROTEIN YABBY 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04690">
        <property type="entry name" value="YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24868">
        <property type="entry name" value="YABBY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YABBY N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="YABBY protein C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH15374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="162" mass="18049" checksum="7EC3A0AACD049EA5" modified="2024-05-29" version="1">MDLVPPSEHLCYVRCNFCNTVLAVGIPCKRLLDTVTVKCGHCSNLSFLSTRPPLQGQCLDHPASLQAAFGNTDYNKKGQSSSSSSSTSSEPASPKAPFVVKPPEKKHRLPSAYNRFMKEEIQRIKTANPEIPHREAFSAAAKNVHSSCGFWIHLRHKQRWCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYQ8</accession>
    <name>A0AAF0XYQ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0935260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYQ8"/>
    <dbReference type="KEGG" id="dcr:108202985"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF20">
        <property type="entry name" value="FAD SYNTHETASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAD synthetase" evidence="10">
        <location>
            <begin position="163"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH15717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="357" mass="39567" checksum="12B04BEB0AB80BF4" modified="2024-05-29" version="1">MLSGSFMTHCILDYDNLHSKLHLKSQLGYVSKKKLNQFSSSSFDFGRPLVSILPNSPFAKNPIISHSLSQQEDDGEPPSERSSAVAGGIVALGKFEALHIGHRELAIQASRVGIPFLLSFVGMAEVLGWEFRAPIVAKCDRKRILSSWAPYCGNVTPTEFEVDFSKVRSLTPRQFVEKLSKNLGVRGVVAGKNYRFGYRASGDASELVNLSEKYGMEAYIINSVMDKEQDSRVIGPDNPKEHGQVSSTRVRYALGRGDMKYVSELLGRQHRLMLMMNDKERFARVNNRISASNSCILNLSPKVGLYENCSVVIGDENFVPCLVTIDTTHIHLEMDDVETCILIVSQDVSLLGIDFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1T9</accession>
    <name>A0AAF0Y1T9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">Poor homologous synapsis 1 PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0936125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y1T9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057619">
        <property type="entry name" value="PH_PHS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25349">
        <property type="entry name" value="PH_PHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poor homologous synapsis 1 PH" evidence="2">
        <location>
            <begin position="28"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="32820" checksum="6D413BA6864882AE" modified="2024-05-29" version="1">MAGNSSASMELAIIDKNKANLSSIRDLYEVEYSRFFTPHSHSPDSSSSHPSLTPLRSSRGSWISSDILASLRLFAAAFDRRGFVLLVSLGRKVLEEHYISKLHITWPQVSCLSGYPARGSRVVLLSYKDSAGQIHKFALRFLSVYETEQFISSLKEILDDSSDVGEPSSNFGSAQSPPSECVISSGSPYRPTKDWSPAPSTNTQSQRMSPILNPDALQTSNPPEPIYYNPVEATHPNFPPSFTSLLLNCNPAVHQAAAQVQPSLSEDADLKDQIARYMEDSSFQGMSASYRPGHDFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AI88</accession>
    <name>A0AAF1AI88_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0100386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AI88"/>
    <dbReference type="KEGG" id="dcr:108205025"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000025">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF27">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="6"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG81241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="169" mass="19247" checksum="126E7B1C5DE31758" modified="2024-05-29" version="1">MASETNKNPVSVYDFTVKDAMGKDVDLNIYKGKVLLIVNVASKCGMTNSNYTELNQLYEKYKDQGFEILAFPCNQFGEEEPGSSDQILEFVCTRFKSEFPIFDKIEVNGDNTAPLYKFLKTGKWGIIGDEIQWNFAKFLVNKDGQAVDRYYPTTSPLTIERDVRNLLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKZ5</accession>
    <name>A0AAF1AKZ5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205744</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKZ5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF9">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG86533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="352" mass="38599" checksum="50348082F8998D53" modified="2024-05-29" version="1">MIWMLQILITTAEPQVPCYFIFGDSLADNGNNNALATQAKVNYPPYGVDFPGGIATGRFTNGENAADILGKLLGFLNYTLPHATATGPEIVKGLNYASGGAGILKRTGRNLGEHINLDLQLKHHGMIVSKIMSLQGNNATFTKDYLSKCIYTVGMGSNDYINNYFMPDKYNTSKRYTPDQYADLLIHKYSRQLESLYIHGARKVVVFGLGLIGCTPAKVSDFGANASGCVDKINAAVTLFNERLKPIIDELNNKFGSAKYIYINTTNISLGDPSSIGFTVFWAPCCKPSVDFGKGQCKPNQPPCNDRSKYIFWDGFHPTEKVHMGTASRAYIAASPLDSYPMDIRSLALLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMA0</accession>
    <name>A0AAF1AMA0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0207176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMA0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044:SF29">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG87943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="106" mass="12207" checksum="7D17EB74690C0316" modified="2024-05-29" version="1">MESMMFGVPVIAMPMHIDQPFNTVVVKEVGVGQEVERDEDGRFRREEIAKVIRNVVIEKSGEIVRRKAKEMREMIREKGEKEIDEVVGELVNLCKEKKDKVTSSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMW4</accession>
    <name>A0AAF1AMW4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zeta toxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0208290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMW4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044802">
        <property type="entry name" value="NADKc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010488">
        <property type="entry name" value="Zeta_toxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153">
        <property type="entry name" value="CALMODULIN CALCIUM-DEPENDENT NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153:SF13">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06414">
        <property type="entry name" value="Zeta_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zeta toxin" evidence="3">
        <location>
            <begin position="177"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="482" mass="54823" checksum="7BE937C2E648EF7C" modified="2024-05-29" version="1">MHYRLKRIKDSRIVPRIRVSDTGQPVRLERFSHYVARQMGFADKRDCPHLCKLASDYIKKTDGVEENIYAFFSHDPEVDSLFIKLVEEFERCILSYFAFHWRHAHTMISQVLGSGSGPKKKFKHIVMAATREQRFERVLKNLKVARVFNTLVEEMKAIGLAANDDSTCTDVMVPMAHKDRSPVLLFMGGGMGAGKSTVLKDILQEPFWAGAAGNAVVIEADAFKESDVIYRALSSRGHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRRRYRMGVGYKVEDGNVIENYWEHCDEELEPDGTKKRRPYRIELVGVVCDAYMAVVRGIRRAIMCRRAVRVRSQLTSHKRFANAFSTYCNLVDNARLYCTNALEGPPKLIGWKDKERNLLVDPEEIHCLKSIGLLNEAADSVHELYKPGHEMQVGAVWNDIVLSPSRMLIQQELKFHIQQVENMAAQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AN57</accession>
    <name>A0AAF1AN57_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cation/H+ exchanger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0205467</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AN57"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000003">
        <property type="entry name" value="Cation/H(+) antiporter 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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                <person name="Simon P."/>
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</entry>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF17">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9724" checksum="C7446FFE7B19D961" modified="2024-05-29" version="1">MIVQKFKDIQDSSDPHFRFEYRTDSHNHLTQLFWADGDGRKNYEVFGDAVSFDATYRTNRYSGLCLTIYSSVYKLATNLFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AST9</accession>
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        </recommendedName>
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    <organism evidence="4 5">
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG91005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nucleotidyltransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
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    </dbReference>
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    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="460" mass="50356" checksum="C79AAAB88ADA3C4B" modified="2024-05-29" version="1">MACNGTGGCDSGCYKDENDSTNGVLPSVTNSSNTNLCLKCKSSEIIAGGTGFSGGTNMKLCADCFRGNLYGKFKLAVSANGMISPSDNVLVAFSGGTSSRVALQFVHEMQVKSQKNFDASRDRSLPVFGVGVAFVDESAFHTLPSHELDKEIEEIKLIVDELSPPTKAFHVIPIESICLSNSNSAKDNLNELLNAVSDKTGKEDLMVQLRMMSLQKTALENGYTKLVLGSCTSRLACHVIASTVKGQGYSLAADIQYVDSRWEIPVVLPIRDCTLQELNMLCKLESLKTMELHNGSRAGINGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNLQLASQRRRKKFNLKSNELLPPESYCPLCSSPLDKNSISNLAFENGHTSYGSFVPKCCSSCQFQILPKEPLHMEQFYSLLPQPITDRAKDGSCRSQRSIREQIQDCLLSDNEDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU22</accession>
    <name>A0AAF1AU22_DAUCS</name>
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        </submittedName>
    </protein>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="13395" checksum="6E16DFB4D536F7FC" modified="2024-05-29" version="1">MISGLTLLTSLRGLRGNIFAFRLFISPIDNQICTGFASHSHTFSLYNCRPTFRGIMMRNECTEDFSGLPYLYALLHCLICAWIWSMLAVYITSNLPRSHHLLWRQPQNGCSCTCTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU64</accession>
    <name>A0AAF1AU64_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0314401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
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        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45764:SF34">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="31"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG95099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="17338" checksum="331BB49356D03DBA" modified="2024-05-29" version="1">MTPQRQNGSGSDKFDACNRMRLLISDDDDFDENKRKRMQSNREAARRSRMKKQQHVHELITEIGQLQNQCKVIMSKINQVTNMFLGVVSENNASRAQLSDMTKRFHLLKSVVQFVEEAEDLGIDVSDVLMESPKFPCPKQQVPTSANMFDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX59</accession>
    <name>A0AAF1AX59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAC-A/B domain-containing protein</fullName>
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    </gene>
    <organism evidence="4 5">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May promote appropriate targeting of ribosome-nascent polypeptide complexes.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AX59"/>
    <dbReference type="KEGG" id="dcr:108215764"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000002">
        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000006">
        <property type="entry name" value="Putative nascent polypeptide-associated complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="3">
        <location>
            <begin position="58"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG98159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="21692" checksum="E0121EBDEAA42ADB" modified="2024-05-29" version="1">MTAQTQEELLAAHLEQQIIDEPVVEDDEEDDEDDDEDDDNDEVDVEGQGDASGRSKQSRSEKKSRKAMLKLGMKAIPGVSRVTVKKSKNILFVISKPDVFKSPNSDTYVIFGEAKIEDLSSQLQSQAAEQFKAPNLSNVIPKAEPSVIAQDDEDVDETGVEPKDIELVMTQAGVSRSKAVKALKAADGDIVSAIMELTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZR9</accession>
    <name>A0AAF1AZR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0417940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AZR9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF522">
        <property type="entry name" value="REVERSE TRANSCRIPTASE TY1_COPIA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="4">
        <location>
            <begin position="94"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="3">
        <location>
            <begin position="153"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG98596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="63919" checksum="7EE8DB0377DEF4F2" modified="2024-05-29" version="1">MANHGESSQTAFNSHDSHSNSQNTARKGHAAEQCFKLVGYPDWYQNFKAKPQQSRIAANVNINDQPGILGSNPQANSYTADTSQVDNKMVSAFVSQVGTVMLTPSITLQHVLYVPEFKHNLLSVGKLLDQNNLIAKFDQDKCFFQDHKSQIIQASSQDYSLFTKMTGKSFTAIVVYVDDLLVTGNDLDAINSIKTLLHQKFTIKDLGDLKYFLGVEVLRTSDGIHLNQRKYILDLLAAAHLSDCNSADFPLSKGLHLSTNDVPKLPDPEKYRRIIGKLLYLNLTRPDISYAVQQLSQFLQAPAQPHYEAALHVLRYLKGTLNVGLFYKADCPLHLTAYSDADWGTCSFSAKSLSGYAVFLGSSLISWKTKKQKTVSKSSAESEYRSMSYTTSELIWIEGLLQDLHSVSLAVNSVSQFSILFVYKHQLHELISTVLHFILPNTQLLIIFQSECLEIRNFRYTLSVPINSIQFPWDVPSILYIPKIAIFYNIKHYYTHYILPLSPFYNNKNTITPTTFLHYLKSIIKKYMSPTNLPTFHLTLLISYTFSWSPCPIPMYITHWDGGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1Q8</accession>
    <name>A0AAF1B1Q8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0520695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the oleosin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1Q8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012511">
        <property type="term" value="C:monolayer-surrounded lipid storage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000136">
        <property type="entry name" value="Oleosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203">
        <property type="entry name" value="OLEOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203:SF25">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B975"/>
    <dbReference type="KEGG" id="dcr:108199361"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000197">
        <property type="entry name" value="Glutathione S-transferase U10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF676">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="89"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH11044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="217" mass="25340" checksum="72129D5E63A8B07A" modified="2024-05-29" version="1">MGDQEVKLFGLWASPFSKRVETALKIKGIKYEYFEEDLSNKSSELLKYNPVHKKLPVLLHNGKPVCESLVILEYIDETWKSERSLLPEDPYARAMARFWAKFIDDKCIAAMWSIRRSSERPEKGIEELTEHLKTLENELKGKKFFGGDEIGLVDITANLIALWLGVLLELKGIELLTKEKFPRLCEWIDYYLDHQIIKETLPDRENYREYLSNVLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAT2</accession>
    <name>A0AAF1BAT2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0729064</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAT2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000003">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="421"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH09606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="85630" checksum="1AEE924D08224CE0" modified="2024-05-29" version="1">MPFGLTNAPATFQALMNEVFAPYLRKFVLVFFDDILVYSKNIHEHQTHLQMVFEVLRKNQLFIRHKKCEFAVKQIEYLGHVVSQDGVVVDQQKIEAMLQWPRPGNVKALRGFLGLTCYYRRFVRGYGEISKPLTQFLKKGGFQWSDSAEIAFQQLKNAMSTTPVLRMPDFEQKFIIETDASHTGIGAVLMQAGQPISFLSKALAPKHLGLSTYEKELLAILMAVEKWRTYVLGQKFIIKTDHQALKHFLDQTLTTILQQKWLSKMLGYDYEIIYKKGKDNVVADALSRATENQSFCASIVVTVASWKEDLKASYVDDNWAEDLFAQEAILGALPADYTNREGLLVKEGRYYVGSSTNLRNKIIETLHNGLEGGHSGVQATVKRIDARFVWPELKKDVSKWVSECDICQRNKSEHVPSPGLLQPLPIPNEVWETISLDFVEGLPKSGGKNVILVVVDKLSNYCHLMALSHPYSANVVAQLMLDNVVKLHGVPTAIISDRDPIFISTFWKELFKSMGTKLKLNTAYHPQTDGQTERVNQCIEMYLRCLCGQKPKQWSNWLPMAEWWYNTTFHSAIGMSPYKALYGRNPPSINYQMPAVKVPVVDEFIRDRIEIQKLLKSNLVKAQERMVWYANRKRTDRSFEAGDEVFLKLQPNHKLSAKYYGPFKVLQKIGKVAYRLELPPGSKIHLTFHVSQLKKRIGTGKPVQTALPEMEVESAVKIPERVLQRMLVKRGNLPATMVLVQWATETPEEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCL9</accession>
    <name>A0AAF1BCL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Brf1 TBP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0830812</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCL9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000066">
        <property type="entry name" value="Transcription factor IIIB subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Transcription factor TFIIB cyclin-like" evidence="10">
        <location>
            <begin position="96"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Brf1 TBP-binding" evidence="11">
        <location>
            <begin position="353"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="505"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="534"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH11331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="61983" checksum="616BCD94EDF81C57" modified="2024-05-29" version="1">MQWMLSSVFNDDDSNVLRRARNFYRIALERNFTKDVQSILLRQLVLYVACRTSEPDPKPYLLIEFAEFLGRNVYELGAVYLTLVQQLSLQDVQSIQKAVDPSLFIHRFANRLLGQTDVNVETTALKIIASMKRDWMQTGRKPSGLCGAALYISAISHGYSYTKSHIVKVVHICEATLTKRLIEFENTESGSLTVCLLMSSPKKQKNMKRSLGHWAKPPLASNPPIRVKFFCKHKGELTFAHGLCRECYAEFMNFSGGLDGESEPPAFQRAQHERMEKEYAEKNAVDSSCDHMAIQGQNSNYIPGESRDENLHSSEHVNAGGATKEVPIDDASYSKLQGADYISAESPASETLSDIEDLEVDNYIHTAEEMQYKKIIWEELNREYVEEQLAKEAAAAAAKEACEAHMRNCPEDMEDARKLAAEVAAAMEQSKKERRQKRAAEAKNAAPPRTAAEATRQMLAKKKLDSKINYDVLDELFNDSVSPDNKKSRLNDEVYASGTEYVKQNMKENDSELLPENENEGGPEEFSEGLQYGNEEDYIDDYGYDEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD96</accession>
    <name>A0AAF1BD96_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0934722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BD96"/>
    <dbReference type="KEGG" id="dcr:108202479"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF35">
        <property type="entry name" value="ZINC TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042225664" evidence="9">
        <location>
            <begin position="23"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH15185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="339" mass="35852" checksum="D78B233303FA70DE" modified="2024-05-29" version="1">MSPHHFVLFSFVLLMIPNLILAECTCDSEAEVRDKNLALKYKIVAIASILGASAIGVCIPFLGKIIPALNPEKNLFFLVKAFAAGVILATGFIHVLPDAFENLTSPCLDQNPWGKFPFTGFIAMVAAIGTLMIDSYATSYYSESHLSNKAQAAVGDVEDSGTHVHGHVHGGMASEASQQLIRQCVVSQVLELGILVHSVIIGISLGASESPETIKPLVAALTFHQFFEGMGLGGCITQAKFKSRAVAVMALFFSLTTPVGICIGLGIANSYDENSTTALIVEGCFNAASAGILIYMALVDLLAVDFMSPKLQNDGKLRLGANVSLLLGAGCMSLLAKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BG70</accession>
    <name>A0AAF1BG70_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_0936109</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BG70"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000021">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF90">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042234741" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="19"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="295"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH16553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="618" mass="68691" checksum="7EE5EAEE5391C325" modified="2024-05-29" version="1">MEVLGGFLILMLLQGCFASLSPSGVNYEVVALMAIKNDLIDPHNMLENWDITFVDPCSWRMITCTPDGYVSVLGLPSQNLSGTLSPGIGNLTNLQYVLMQNNAISGSIPAAIGKLEKLQTLDLSSNKLHGEIPSSFGDLKNLNYLRLNNNSLTGKIPDSLSGLGALTLVDMSFNNFSAPVPKLPARTFRITGNPLICPEGAENNCSVIYQKLPSFPPDDQIESGRKDHHHIAIAMGTSFGAALLIIIIIGSLVWWRYRHNKQIFFDVHDQYDPDVCLGHLRRYTFKELRTATDHFNSKNILGRGGFGIVYKGCLNDGTTVAVKRLKEYNTLGGEIQFQTEVELISLALHRNLLRLLGFCTTESERILVYPYMVNGSVASRLRDHVLGRPILDWSRRKQIALGTARGLFYLHEHCDPGIIHRDVKAANILLDEDFEAVVGDFGLAKLLDHRESHVSTAVRGTVGHIAPEYLSTGQSSDKTDVFGFGILLLELITGQKALDFGRAASQKVVMLDWVKKLRQDGKLDLIVDKELKKDYDKIELEEIVQVALLCTQFNPSNRPKMSEVLRMLEGDGLAEKWEASQRDETPSCRTSETTPERYSDFIEESSLVVEAMELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WTS6</accession>
    <name>A0A161WTS6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_010859</name>
        <name evidence="3" type="ORF">DCAR_0312330</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG93051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WTS6"/>
    <dbReference type="STRING" id="79200.A0A161WTS6"/>
    <dbReference type="Gramene" id="KZN02105">
        <property type="protein sequence ID" value="KZN02105"/>
        <property type="gene ID" value="DCAR_010859"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210414"/>
    <dbReference type="OMA" id="WDIKFKD"/>
    <dbReference type="OrthoDB" id="742916at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF25">
        <property type="entry name" value="BNAA09G04570D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN02105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="519" mass="60176" checksum="F5DB587567DD618D" modified="2016-07-06" version="1">MVAVFNKELLSWYLLTLKLKETVEAGVSTQQASALYRSEQTYLLEHHEESSSIREIDAFGSGPRPAISEWVININAKLEQARQEDLAGSWGRVSIYRVPQYLKGGDNKAYIPQIVSLGPYHHGKKRHRNMDRHKWRSFYQILKRTNQDLKLYLDSMKELEERTRACYEGKINLSSNEFVEMMVLDGCFILDLFRGASEGFQQIGYSRNDPVFAMRGSMHSIQRDMIMLENQIPLFVLDRLLGLQLGHPDQKGLVARLALSFFDPLMPTDEPLTKSNRNKMEHSVGYTNTFDPLSDQGLHCLDVFRQSLLRRGPSPLPQVWKKQWSHVCRVADKRRQQLTHCVTELRESGVKFKKKKTDRFWDITFSNGLLQIPRLLIHDGTKSLFLNLIAFEQCHLDCGNEITSYVIFMNNMINSTEDVGYLHYCGIIEHWLGSDHEVAELFNKLCQEVVFDINDSYHSRLSAEINKYHDHRWNAWQATLKHNYFNNPWAIISFVAAVVLLILTFAQTIYGVFGYYHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XA41</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_023175</name>
        <name evidence="3" type="ORF">DCAR_0622832</name>
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    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XA41"/>
    <dbReference type="Gramene" id="KZM89462">
        <property type="protein sequence ID" value="KZM89462"/>
        <property type="gene ID" value="DCAR_023175"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM89463">
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        <property type="gene ID" value="DCAR_023174"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM89462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="17467" checksum="E505D4EC6A6DA436" modified="2016-07-06" version="1">MDVAKMKDGDDKKVTGLGVDLEKKMVLDPEKKMVLDPEKKMVLDPNKKMVLDPKKKMVVNRSYDEDLRLFERMCRNFCLEEIEAAGYTLLEDDKTTTTRPAASASAKRCQPKEEEEQQQEEEIEVVYDFKRPKLPLPPGRPFVRDGDVYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XC95</accession>
    <name>A0A161XC95_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XC95"/>
    <dbReference type="Gramene" id="KZM90243">
        <property type="protein sequence ID" value="KZM90243"/>
        <property type="gene ID" value="DCAR_022392"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="86" mass="9246" checksum="7C10FB7FD0FE20C3" modified="2016-07-06" version="1">MNSGQASELQTRVSDVLRRRGEESGLQAFLRARCINTQGQQGYVGISRHQNATCTSTAQQMPGSNLLASGNGAYHGFNKNGDTNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XV59</accession>
    <name>A0A161XV59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallothionein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_018908</name>
        <name evidence="7" type="ORF">DCAR_0521626</name>
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    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallothionein superfamily. Type 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XV59"/>
    <dbReference type="STRING" id="79200.A0A161XV59"/>
    <dbReference type="Gramene" id="KZM95666">
        <property type="protein sequence ID" value="KZM95666"/>
        <property type="gene ID" value="DCAR_018908"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220428"/>
    <dbReference type="OMA" id="NEGSEMN"/>
    <dbReference type="OrthoDB" id="1111048at2759"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000347">
        <property type="entry name" value="Metalthion_15p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543:SF37">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01439">
        <property type="entry name" value="Metallothio_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM95666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH02237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="7627" checksum="9C387AE89063495F" modified="2016-07-06" version="1">MSCCGGKCGCGSDCKCGSGCNGCGMYPNMENTAAATPLIDGVAPTMMYAEGSEMSFGAEGGHACKCGSNCTCNPCKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XYB7</accession>
    <name>A0A161XYB7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_010471</name>
        <name evidence="2" type="ORF">DCAR_0311905</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XYB7"/>
    <dbReference type="STRING" id="79200.A0A161XYB7"/>
    <dbReference type="Gramene" id="KZN01717">
        <property type="protein sequence ID" value="KZN01717"/>
        <property type="gene ID" value="DCAR_010471"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212436"/>
    <dbReference type="OMA" id="YMEMEQN"/>
    <dbReference type="OrthoDB" id="696337at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265">
        <property type="entry name" value="AVR9/CF-9 RAPIDLY ELICITED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265:SF26">
        <property type="entry name" value="OS06G0554600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN01717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="197" mass="23053" checksum="895E3E3C1392EECA" modified="2016-07-06" version="1">MEVEECSSIVATKMSSTMSIVLCMLKKGFSRSKLKIFDLHTKMKHEKHASTSMSSVLLHHHYAASSICRSTDVAMSFVSPRKDYEFSCSNTPLIRRRKRYYQYRHSYNHPKVFVDDLRWSEYESVEASPAFSLPGFGRSPVRVRQLRVSDSPFSIKDAEENTEQLDRAAEDFIRKFYEELEKQRRMAPPATYYRRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZK58</accession>
    <name>A0A161ZK58_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_024586</name>
        <name evidence="8" type="ORF">DCAR_0727884</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZK58"/>
    <dbReference type="STRING" id="79200.A0A161ZK58"/>
    <dbReference type="Gramene" id="KZM87452">
        <property type="protein sequence ID" value="KZM87452"/>
        <property type="gene ID" value="DCAR_024586"/>
    </dbReference>
    <dbReference type="OMA" id="YYLPPHD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF68">
        <property type="entry name" value="BASIC 7S GLOBULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007830442" description="Peptidase A1 domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="6">
        <location>
            <begin position="43"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM87452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH08443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="433" mass="46998" checksum="8D86D7CAA3A4D3FE" modified="2016-07-06" version="1">MSSALHYTLLILLLAVALSSAAAQAKPKAFLYFIKKDPEALQYSTTLEFSTQVNFVDVVLDLGGEHTWFDCDSYDLPTYKPISCGTTKCNNVKSNGCIGCNLPPKPGCTNNTCGVSSYNPFTNDLYAQGLGEINLFSTETNGLAVGELHKLPKPFPFSCADSDLLRGLSNATKGMTGLANTVTSLHSQWSSKFKLPHKFALCLPSTSELNPGHMFIGGGPYYFPPYTKNIATQLLTTKLVINPVSTAPIYTEGDHSDEYFIDVTSISVDNRPVSVNASLLSIDMNGYGGTKFTTVTPYTKLETSIYKALVGDFKKAASLRKMKRVASVAPFSACFRASSVATSQTGPVVPYIDIGLAGNQHWRFYGANSMVSLKKDVLCLAFVDGGSKPRTSVVIGGHQMENYLIEFDLVSSKLVISTSLLFRNTTCQQFRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZKH8</accession>
    <name>A0A161ZKH8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA synthetases class I catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_023644</name>
        <name evidence="9" type="ORF">DCAR_0727122</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZKH8"/>
    <dbReference type="STRING" id="79200.A0A161ZKH8"/>
    <dbReference type="Gramene" id="KZM86510">
        <property type="protein sequence ID" value="KZM86510"/>
        <property type="gene ID" value="DCAR_023644"/>
    </dbReference>
    <dbReference type="OMA" id="AANECIH"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF26">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE 1, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="7">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM86510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="36546" checksum="944A816334DF91BF" modified="2016-07-06" version="1">MVLLPGQVNFVSPLEFFTMHEFNVRSPALLTKQKEVFKPRVPGKVGMYVCGVTTYDFSHIGTARAFVTFDILYRIWLPHQPTVGLWDSNFVSGLLWWVLHFCLSELTYKTSRTGDRTLNPTPALYKTSTLQDCQDALSSSREGSADTAGGNKVHITDAANECIHNLQNDVKKMSCDLHTYSLLNSALPKALKLINSSLTLVKVLGQLKSKALIRSGFEDEYIEDQIKERALARKNKEFSRGDKIRSYLASKGIALMDAGTETICRPCVPVQQEQLAVPKEAKPCKDTEKQQQAQLVQVKSEEPLLNEQEKSSCVEAVKENLTMPTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZP93</accession>
    <name>A0A161ZP93_DAUCS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_025265</name>
        <name evidence="4" type="ORF">DCAR_0729013</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZP93"/>
    <dbReference type="STRING" id="79200.A0A161ZP93"/>
    <dbReference type="Gramene" id="KZM88190">
        <property type="protein sequence ID" value="KZM88190"/>
        <property type="gene ID" value="DCAR_025265"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196437"/>
    <dbReference type="OMA" id="DESDHTA"/>
    <dbReference type="OrthoDB" id="1920951at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33868">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33868:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM88190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="478" mass="53316" checksum="01E9FAF8A2464670" modified="2016-07-06" version="1">MAAAEARAAWQRTANRCFVQEDAKRAPKLACCPSSGATAKQVDSGPASATDGQDYCVPGFVPHSNNPSYMNVSPDTKWWLQMQPNYGYKKGFLNDQLNTMDAEVDIHGVGFASLNVKTSRVHLNNVGTLVTRNLNSDPTHGSEGRVCASFIKKERSVNKQELKAEYAKGVQESVKMKDGKESYEFVEMDHIDGASLNTSDEWCLDSGSPLIGSGKKDPWWKTADSDELASFVTQRSSVHVENCDLPQPQVNHIRRDPNVRARLPNYNGSFNSSVDMKPQNADRSNRSSYRSGGGTPKSTCGSNWSVEGNSKSGVDSPLSYNTTRSGKAETNTSDKDDCKAQLLEALRHSQTRAREAEMAAKQAYAEKEHVVKLFFRQASEFFAYKQWFQLLQLENLYYQIKNNKNHPMSDLFPSVLPWMPIKNRKMRKKAIKGKCSKQGRSRCNIHKYTFMFALGLSLVGAGLFLGWTIGWMFPALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A6T3</accession>
    <name>A0A162A6T3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_019502</name>
        <name evidence="3" type="ORF">DCAR_0522312</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A6T3"/>
    <dbReference type="STRING" id="79200.A0A162A6T3"/>
    <dbReference type="Gramene" id="KZM96260">
        <property type="protein sequence ID" value="KZM96260"/>
        <property type="gene ID" value="DCAR_019502"/>
    </dbReference>
    <dbReference type="OMA" id="VRAEIIY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF93">
        <property type="entry name" value="F-BOX_FBD_LRR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM96260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="416" mass="47656" checksum="24385E3D28612B6F" modified="2016-07-06" version="1">MEMDIDYISNLPEHITDKILSCLSLRDAVRTSILSRKWRYKWISLPQLVFDNQSLLISSQDQTLIRNKLINIVDHVLLLHSGPIQKFKLSHRDLQGVNDIDRWILFLSRGSLKEFILEIWKGHRYKLPASLYSCQKLIHLEIFNCMIRPPTPFNGFKNLKSLDLQHITMEQDVFDNLISSCTLLERLTLMNFDGFSQLNIHAPYLLFFDIGGVFEDVNFNATNNLAIVSIGLYATAGYDQNPALGSTGNMIKFFAPLPRIQRLEVQSFFLKYLAAGKVPERLPTPCIELNYLSFRINFCDRAESLGALCILRSSPNLQELEILVRPDDQAVDGRDARISEEYFHNCPFNQLQLVKLVGISYAKQELNFVNFLLANSPVLEKMTIKPAAAIGGCELLKELLRFRRASVRAEIIYLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A8A3</accession>
    <name>A0A162A8A3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_015688</name>
        <name evidence="3" type="ORF">DCAR_0415027</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A8A3"/>
    <dbReference type="Gramene" id="KZM96950">
        <property type="protein sequence ID" value="KZM96950"/>
        <property type="gene ID" value="DCAR_015688"/>
    </dbReference>
    <dbReference type="OMA" id="IIREAFW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM96950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="58" mass="6322" checksum="371AFC77276C99DB" modified="2016-07-06" version="1">MGIFSGMLGCFSPSSSARVNDKENVKDKPNCLSSNHKSKSKAPIPVSYFPVNSRISYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A997</accession>
    <name>A0A162A997_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_014686</name>
        <name evidence="4" type="ORF">DCAR_0416213</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A997"/>
    <dbReference type="Gramene" id="KZM97952">
        <property type="protein sequence ID" value="KZM97952"/>
        <property type="gene ID" value="DCAR_014686"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831952" evidence="2">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM97952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10568" checksum="0A6E65F7D404E8BD" modified="2016-07-06" version="1">MRLVGLLFAVVMVHLILTTLECRALARSDIFKNHRHHEQRDSTSVAGGEGVTTSFLNSGNDLSCSNSSFNQQSICANSTGNDDKRLVPTGSNPLHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AAM2</accession>
    <name>A0A162AAM2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DELLA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_014176</name>
        <name evidence="10" type="ORF">DCAR_0416765</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DELLA motif is required for its GA-induced degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GRAS family. DELLA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AAM2"/>
    <dbReference type="STRING" id="79200.A0A162AAM2"/>
    <dbReference type="Gramene" id="KZM98462">
        <property type="protein sequence ID" value="KZM98462"/>
        <property type="gene ID" value="DCAR_014176"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218420"/>
    <dbReference type="OMA" id="ICNVVAY"/>
    <dbReference type="OrthoDB" id="761920at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1290">
        <property type="entry name" value="Transcriptional regulator DELLA, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038088">
        <property type="entry name" value="DELLA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021914">
        <property type="entry name" value="TF_DELLA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12041">
        <property type="entry name" value="DELLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01129">
        <property type="entry name" value="DELLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0939">Gibberellin signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transcriptional factor DELLA N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="6">
        <location>
            <begin position="231"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="6">
        <location>
            <begin position="310"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="6">
        <location>
            <begin position="449"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LxCxE motif" evidence="6">
        <location>
            <begin position="166"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="6">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LXXLL motif" evidence="6">
        <location>
            <begin position="362"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM98462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="530" mass="58351" checksum="AB96EF51D78809FA" modified="2016-07-06" version="1">MVTKRDRPPEKGQGSSAAKMWIEDQPDTMDELLAVLGYKVRASDMTVIAKKLEQLEVAMGIDTVHGATHLSETTHYNPADISTWVESMLTELSPSVSETHSSLIHDNHSEYDLRAIPGGAIYGGDSSTSSGSKRMKVSESCVVESGPGPLVVDSQEAGVRLVHTLLACAEAIQVDDLKLADALVKHVSLLSTSQVGSMRKVASYFAHALARKIYKIFPKDSLEVSFLDMYQMHFYESCPYLKFAHFTANQAILEAFAGCNRVHVIDFGLKQGMQWPALLQALALRNGGPPSFRLTGIGPPQPDNTDALQEVGYKLAQLAESIGIEFEFRGFVANSLADIDASMLNIRPREEEAVAVNSVFELHRLLARPGAIEKVLNSIKKMNPKIVTVVEQESNHNSPVFLDRFTQALHYYSTMFDSLETTGFTQTNDTQDLLMSDVYLGRQICNVVSCEGPDRVERHETLTQWRTRMCEAGFDSVHLGSNAYKQASMLLALFAGGDGYQVEENDGSLMLGWHTRSLIATSAWQLVGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AGL9</accession>
    <name>A0A162AGL9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AGL9"/>
    <dbReference type="Gramene" id="KZN08806">
        <property type="protein sequence ID" value="KZN08806"/>
        <property type="gene ID" value="DCAR_001462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="76" mass="8488" checksum="24F15503694FA4E3" modified="2016-07-06" version="1">MPQEVRSIIQQIMILKKRCGNVSSTNDPGFAAQFMGEMGFQIVPQGQAQAEMEINDFILEDEIKDVSEDEAVVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AWP9</accession>
    <name>A0A162AWP9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glabrous enhancer-binding protein-like DBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0208738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GeBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AWP9"/>
    <dbReference type="Gramene" id="KZN06903">
        <property type="protein sequence ID" value="KZN06903"/>
        <property type="gene ID" value="DCAR_007740"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205970"/>
    <dbReference type="OrthoDB" id="661680at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007592">
        <property type="entry name" value="GEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053932">
        <property type="entry name" value="GeBP-like_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662">
        <property type="entry name" value="BNAANNG10740D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662:SF33">
        <property type="entry name" value="DNA-BINDING STOREKEEPER PROTEIN TRANSCRIPTIONAL REGULATOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04504">
        <property type="entry name" value="GeBP-like_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glabrous enhancer-binding protein-like DBD" evidence="3">
        <location>
            <begin position="95"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="353" mass="39216" checksum="727BA777CD08EC41" modified="2016-07-06" version="1">MARKRPSRPIDILAAAAANDQSRHPDNHADRSGSGSESNKLSGSDPDSVVIPKPIEQNSPNRKSSRKRSLPKHRLLDESEGSSKKARVGSIGSVNRVWSEQDEIGLLQGMIEFKSRKGEDPYSDMNAFHSFVQDSISVHVSKKQVVDKIRKLKYKYLANAERGDVVFVKPHDETVFELSKVIWGGDGSVRVTKDKEDGNVVENGMNENGVEVDDEEGGNVVENGVNEKFVVVENGIEVDGEEGGNVVENVVNGKVDVVENGVEVSDEREDGGRDFKEVYPYLSIGWESDFTCSSLFKNYLTGNMHSVGSGKLREMDKKWKNLYMEELKLYVKKLDLVKEHAQAVLDRLSTSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SUG9</accession>
    <name>A0A164SUG9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_023788</name>
        <name evidence="13" type="ORF">DCAR_0727288</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SUG9"/>
    <dbReference type="STRING" id="79200.A0A164SUG9"/>
    <dbReference type="Gramene" id="KZM86654">
        <property type="protein sequence ID" value="KZM86654"/>
        <property type="gene ID" value="DCAR_023788"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195614"/>
    <dbReference type="OMA" id="WVNNLVV"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000016">
        <property type="entry name" value="Polygalacturonase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853179" description="Polygalacturonase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM86654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH07854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="41862" checksum="7CB8481227892992" modified="2016-07-06" version="1">MAKLILSCILFFTFTALFSLSNAANTINVIKYGAKPDGKTDSTQAFVKAWVAVCNSAQAVTMYVPQGRFVIRAVEFRGPCKSKINVQIDGTIVAPGDYRALGNTGNWILFIKVNRVSVNGGTLDARGAAFWNCRESGKNCPEGARSITFNWANDIKISGLTSINSQAAHVVINGCNNVMVSNLRLNAPDQSPNTDGIHVQTSTGVTITGTTIKTGDDCVSIGPGTRNLWMERIQCGPGHGVSIGSLGKDANEAGVQNITLINSAFTGSDNGLRIKSWARPTTSFVRNVVYQNIQMRNVENPIIIDQNYCPNNQNCPHQTSGVKITGVTYKNVQGTSASKVAVAFDCSPTQKCTGIKLQDVKLSYQNRDARSTCKNSGGTTSGTSMPNSCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U228</accession>
    <name>A0A164U228_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0102035</name>
        <name evidence="1" type="ORF">DCAR_025409</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88334.1"/>
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</entry>
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                <person name="Zheng Z."/>
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                <person name="Macko-Podgorni A."/>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
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        <recommendedName>
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    </protein>
    <gene>
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    </gene>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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        <property type="molecule type" value="Genomic_DNA"/>
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        </location>
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        </location>
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            <end position="411"/>
        </location>
    </feature>
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        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM88744.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH10206.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="411" mass="45297" checksum="0DDC6721F406BB49" modified="2016-07-06" version="1">MASWFARTIASTLDLDEPEQPKQPQNDDVNQPEDLSDESPPLSPNRSVKEDLSELTKTVTRQFWGVASFLAPPATSNSDPPTDDLEGSEIIPGIRSDFAEIGGRFRSGISKLTDNVASVSEITSKMASNFLQLGDDEEEERQQGDDGAVGVTDVVVDFVKDITVHPDTWLDFPLPDHEDDQDFDMSDAQQEHALAVEQLVPRLAVLRMELCPGYMSEAWFWKIYFILLHPRLDPHDAEVLSTPQILEARALLAHNIKKPTNANPEQDWSSRGASYVKDSTNLQDEEHLSASSTVALESVPIETSAVAVEFPVATVPESETEKHPITSTEIEIVDKSVIEEENVKQNKDQNLRSTSASDIVENNDEDDADEWLKEESSGTGGSKGTTIPLDNDDDDDVSFSDLEEDEGDAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ULR4</accession>
    <name>A0A164ULR4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0730010</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PEX13; forming the PEX13-PEX14 docking complex. Interacts with PEX5 (via WxxxF/Y motifs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ULR4"/>
    <dbReference type="Gramene" id="KZM89057">
        <property type="protein sequence ID" value="KZM89057"/>
        <property type="gene ID" value="DCAR_026132"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196076"/>
    <dbReference type="OMA" id="FHWSHAI"/>
    <dbReference type="OrthoDB" id="441517at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000217">
        <property type="entry name" value="Peroxisomal membrane protein PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054154">
        <property type="entry name" value="PEX14-like_M_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23020">
        <property type="entry name" value="PEX14-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="16">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14 central plants" evidence="18">
        <location>
            <begin position="146"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="17">
        <location>
            <begin position="313"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="90"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="267"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="332"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH10541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="512" mass="55273" checksum="B492BF6B51C66CF4" modified="2016-07-06" version="1">MATQSAPPSNFSDQNPVSEVAKPASEVHQDAKDISPPSVFVNSEPIREDQVQNAVKFLSHPKVKGSPVMYRRSFLERKGLTKEEIDEAFRRVPDPTPTVSTGQPAEDGQLNSSKMQQQSPTQAIQPASNTPIGTVSKGGTSALYHLGWQQALLAVGLLAISGAGTALVFKKAIVPRLKSWIRKVVSEEDEDLANKTNSKPSLAEEAAAAAKSAAAAAADVARASQDMLISKNGEKQYFEELMSLLGVQVQEMKSMSNAIRNLEGKTSISGQEDHGFSLNNSKPPYANGKSDFTPSSARPVSPPASVEPSGAPPHPKSYMEIMAMVQRGERPSNIRDVNDLPPNPNQPVPTPSLAPRPKPWEVGQVQNSSNQALQSQDNGFTQMNGNNSAPWWQQKQSNNVRINDIESVDEYSAGSSGAQTIDRPIQRSWVPPQPPPVAMAEAAAAIRQPKKSTFQSEPLTDDQFLARSSELTNELQRVTKISESGGITEANGESYVPNTEIQTEESNSYVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VYZ7</accession>
    <name>A0A164VYZ7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH05249.1"/>
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        <property type="gene ID" value="DCAR_021578"/>
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    <dbReference type="KEGG" id="dcr:108224629"/>
    <dbReference type="OMA" id="MHQVFPG"/>
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        <property type="term" value="F:thermospermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:polyamine biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="332" mass="37307" checksum="C5EDC7422F44AD38" modified="2016-07-06" version="1">MSTTLTLSTIQKRLSTPLQIQPLPSPPLRNLRRSFKPNLIKISPLRYYPIKAQQTQKPDPEIKPQDGIPIEFVKTLASFKSRYNYIRVIEVSRRSDHPFAGSRLLLLDGPGNIHSISYLFKSLTSTYFDVFAILPPILPPGPLGILGFGAGSAARLILELYPHGVIHGFEIDPDVISVGREFFGLTKMEKKYDDRLFVYVGDALNASVEGGYSGFLIDLFGEGCLIPELQDPGTWEMLKKRLRKGGKMMVNVGGSCVEPEDSRRDGRVIMEETLKAMHKVFPGEVYVMSLGNGKDDSSIAFIGEMPNLESWRKALPKSLKLYVDMWRPYREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WIP7</accession>
    <name>A0A164WIP7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0625533</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_020810"/>
    </dbReference>
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    <dbReference type="OMA" id="GKMSENC"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="487" mass="55757" checksum="4112A50336C53EAC" modified="2016-07-06" version="1">MFNKIIKRGQRKGSKSDNNIDYGATGNRSSGSVSTSNVVVNHASRGGLTAGQGGGGGSAGVVAAEPGSIEVLPLFKDVPVGERQGLFLRKVQVCCFLFEFTDTMKMAREKEIKRQSLSELVDFVQSGSAKISEGNQEEMVRMISVNIFRCFPPAAHENTGSENVEPEEDEPFFEASWPHLQLVYELLLRYVVSSDTDTKIAKRYIDHSFVLKLLDLFDSEDPREREYLKTILHRIYGKFMVHRPFIRKGINNIFYRFIYETQRHNGIGELLEILGSIINGFALPMKEEHKLFLLRALIPLHKPKPVGLYHQQLAYCITQFVEKDHKLADTVIRGLLKYWPVTNCQKEVLFLGELEEVLEATQAGEFQRCMVLLFKRIAHCLNSPHFQVSERALFLWNNEHIVGLITQNRTVILPIIFEALEKNIRSHWNQTVNGLTVNVRKMLSEMDPELFEECQRQFAEKEAKAEELEVQWETKWQRLVAAAEQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WJ38</accession>
    <name>A0A164WJ38_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ycf20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_020794"/>
    </dbReference>
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    <dbReference type="OMA" id="KSCIMPP"/>
    <dbReference type="OrthoDB" id="5493at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007572">
        <property type="entry name" value="Uncharacterised_Ycf20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33787:SF5">
        <property type="entry name" value="YCF20-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04483">
        <property type="entry name" value="DUF565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="155" mass="16967" checksum="7464685C1DDB5F42" modified="2016-07-06" version="1">MSFSMSSTSIGLLKIGLFPGVKVLHSKCAAPVSRSSRRLFKVRAAQNNGGPRRLVDIIRIVPEISSNYFKKPSRRALFGGISLLGGFYVAQTISLSFGALGVNDVIAAVVCVLLTEYVTRFYYSRPKVTFPVALLNNFKMGFTYGLFIDAFKLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WZF9</accession>
    <name>A0A164WZF9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_020151</name>
        <name evidence="19" type="ORF">DCAR_0626294</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WZF9"/>
    <dbReference type="Gramene" id="KZM92484">
        <property type="protein sequence ID" value="KZM92484"/>
        <property type="gene ID" value="DCAR_020151"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227853"/>
    <dbReference type="OMA" id="WNDRFNI"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000413">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000267">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000295">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000402">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF76">
        <property type="entry name" value="OS03G0145102 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854177" description="Protein kinase domain-containing protein" evidence="16">
        <location>
            <begin position="21"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="606"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="685"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="714"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KZM92484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH06865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="967" mass="104508" checksum="FA314538E476FC99" modified="2016-07-06" version="1">MRSLFVHTVLLLLVVGQVRSLNPSLNDDLLGLIVFKADIQDPDGKLESWNEDDNSPCGWMGVRCNPRSNRVTELNLDGFGLSGRIGRGILQLQFLRKLSLAKNNLTGSLAFSLAQLENLRVVDLSQNNLGGPIPGNFFSQCGSLRSISLANNKFSGHIPDRLSSCFTLATLNFSGNQFSGLLPSGIWDMQGLRSLDLSSNLLEGEIPKGIEGANNLREIHLEKNRLTGEVPAGIGNCLLLRSIDFSDNSFSGFIPSTMQMLTLCNNLNLHKNSFLGEVPEWIGDMKSLESLDLSENRLSGPMPISLGKLQSLKLLNLSKNSLSGSLPESIINCVNLVVVDVNQNFLTGKLPSWLFGLSLQKVDFSENNLNGNIDASVKSLTDHSRRSLLVLDISNNSLTGEIPSALGDISSLQYLNLSRNSLTGGIPDSIKNLKALDVLDLSNNQLTGSIPMKIGDAVSLRKLWLEKNFLSGDIPASIEKCTPLTSLILSHNSLTGSIPVAFAKLTNLQTVDLSYNKFSGTLPKQLANLAHLISFNISHNQVQGDLPAGGFFNTISPSSLSGNPSLCGAAVNKSCPAVLPKPIVLNPNSSDSNPDSIPVNPGHKKIILSISALIAIGAAATIIIGVIAITVLNLRVRSTTPHSAAAFTLSGGDGFSSSPTSDGNSGKLVMFSGDPDFSTGAHALLNKDCELGRGGFGAVYRTVLKDGRSVAIKKLTVSSLVKSQDDFEREVKKLGKIHHRNLVALEGYYWTPSLQLLIYEFVSGGSLYKQLHEVSGNSLSWNERFTIILGIAKSLAHLHHQNIIHYNLKSSNILIDSSGEPKVADSGLARLLPMLDRYILSSKIQSALGYMAPEFGCKTVKITEKCDVYGFGVLVLEVVTGRRPVEYMEDDVVVLCDMVRGALEEGRVEDCVDEKLRDKFPPDEAIPVIKLGLICTSQVPSNRPDMAEVINILELIRCPSEGQEELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X6C1</accession>
    <name>A0A164X6C1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0626629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_019870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="64" mass="6936" checksum="B4D22013443A1929" modified="2016-07-06" version="1">MAATDTLLLPFKSRQGLTVKASIHPSFHNHLPHCNTQSSISMSHNLPSTNTINKQLDPTEIAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YAZ9</accession>
    <name>A0A164YAZ9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0520053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZLN9</accession>
    <name>A0A164ZLN9_DAUCS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">DCAR_0522130</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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        <property type="entry name" value="MYB-LIKE PROTEIN X"/>
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    </dbReference>
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        <location>
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            <end position="37"/>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="17286" checksum="7857A09F9136AFAC" modified="2016-07-06" version="1">MLKQSPSRNQRSKEFKVKHALQICLLLAICIWLLYQVRQTCQRKLASEESFGTAKLSTDFGMLKLGRKGLDPQLQEIVTDIKGTATEDAEVEGKLRESKGDNIGVVNDEIDGQDQEKDDEEESEQLEDLIDEDDTEGKGSVEIDSDGEDVDLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z5L4</accession>
    <name>A0A165Z5L4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_013000</name>
        <name evidence="8" type="ORF">DCAR_0418103</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z5L4"/>
    <dbReference type="Gramene" id="KZM99638">
        <property type="protein sequence ID" value="KZM99638"/>
        <property type="gene ID" value="DCAR_013000"/>
    </dbReference>
    <dbReference type="OMA" id="YLFTINP"/>
    <dbReference type="OrthoDB" id="601557at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM99638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG98758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="328" mass="37859" checksum="4F67582583704948" modified="2016-07-06" version="1">MGRAKLNMELIEKAKVRTTTFLTRKKNLTKKAKELATLCGIKTAVIINPPRQVTPQGRPLKLETYPENDDVVLELIDKYKGQSSEDRKKRTSLLPCFFQERNRKVQRALAKLRSTNVRSKYPTWDLRFDNFMEEDLRNVAVFLENKIVDAKGKLGQMKANVSNSNNIYNAYLLQQQQMMLTRERSMEVGNQNGINYLFTINPPQTGFMQVPVPMPMPIQQQNFAFLDHYQDQRMVTFGHDYIGGAPNIEYNANSSTPLYNYPMMTGDSITYANNSLRDPQNYSDEGRQPFAHHQMENHHMRLGSTPHQMVATSHQVDAHAQFDENHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B0H5</accession>
    <name>A0A166B0H5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_010945</name>
        <name evidence="21" type="ORF">DCAR_0312428</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B0H5"/>
    <dbReference type="STRING" id="79200.A0A166B0H5"/>
    <dbReference type="Gramene" id="KZN02191">
        <property type="protein sequence ID" value="KZN02191"/>
        <property type="gene ID" value="DCAR_010945"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215072"/>
    <dbReference type="OMA" id="SCYSEAR"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000481">
        <property type="entry name" value="Wall-associated receptor kinase-like 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000161">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF62">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007870991" description="Protein kinase domain-containing protein" evidence="18">
        <location>
            <begin position="23"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="329"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="682"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KZN02191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG93147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="698" mass="76085" checksum="92817022D09F1D38" modified="2016-07-06" version="1">MILIITTIITTLFLLSSSSSQASHNSSCSRVCGAHKSTQIHVQYPFGFSPGCEIQLACLNSSVKIEQFRVQNVTNDHILVDIPAQCDRNISAIRPLFGRNFAPSSRNSFLLQNCSRPVKECLIETSLLEDRFQVDTCQSVRSNVSCYVEDRDVEFMSFETLEKTGCSELFTAIAVDLFDNRSVDSSVAIEIQTLELGWLLEGPCKCVAQADCVNLTLGGLQGFRCRCKSGFEGDGFVDGGGCRKVSGCSASGYMSGRCGGTTKVGVLFGGIAAGAGIVAAVALIYYCIRKRTSHLKNQLSAKRLINEATGSSTVPLFPYRDVEKATHGFSEKHRLGTGAYGTVYAGKLHNDEWVAIKKLRHRDADGIDQIMNEIKLLSSVSHQNLVRLLGCCIEKGEQILVYEYMPNGTLAQHLQGERGKGLPWTVRLTIATETAHAIAHLHTATHPPIYHRDIKSSNILLDVNFNSKVADFGLSRLGMTDDSHISTAPQGTPGYVDPQYHQNFHLSDKSDVYSFGVVLVEIISAMKVVDFTRPPTEINLAAIAIDKIGKGRVDDIIDPFLEPNRDAWTLSSIHKVAELAFRCLAFHKDMRPSMTEVAEELEHVRLSGWAPIDENIGMGSSVSSSCASPFNGSELSVGGTNIKKAGIGSRRVIVTQREVACLTTMEEVSDSSPVSVHDPWLSDQSSPSTNSLLGNITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C343</accession>
    <name>A0A166C343_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0313543</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166C343"/>
    <dbReference type="Gramene" id="KZN03220">
        <property type="protein sequence ID" value="KZN03220"/>
        <property type="gene ID" value="DCAR_011976"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211687"/>
    <dbReference type="OMA" id="ETSSCCE"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000055">
        <property type="entry name" value="GATA transcription factor 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052138">
        <property type="entry name" value="GATA_ZnFinger_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255:SF4">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG94250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="326" mass="36524" checksum="889A3F1F33D115CF" modified="2016-07-06" version="1">MTPTYLNLLASPPSTSADHGDLLQQTSIFGSNNYQDASSCSYITTHNFFDLETTKNQSDEICRQDIQQSQFRQDLADNLASSHGGSFDIENETSDTGLKFSIWKQEEKYANQESTYNYNNEIKLMSPKMRWMHKMKNDVDSPPLKTTTTLIKLGDQKPPPSSPIGADNTSNTSSNSMISHPIRTCSDCNTTKTPLWRSGPQGPKSLCNACGIRQRKARRAMAAAANDTAFEKETTPPAVIKTKKYNKLKTKTKQEKDKTCVSVAKFKKRSCNLMIEGEDEAGKKVCLEDFLLNLTNNLAYHQVLPQDEKDAAILLMAMSLHHTHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F0D3</accession>
    <name>A0A166F0D3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008029</name>
        <name evidence="3" type="ORF">DCAR_0209072</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F0D3"/>
    <dbReference type="Gramene" id="KZN07192">
        <property type="protein sequence ID" value="KZN07192"/>
        <property type="gene ID" value="DCAR_008029"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044716">
        <property type="entry name" value="LEUNIG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44376:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL COREPRESSOR LEUNIG ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44376">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF FILAMENTOUS GROWTH FLO8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN07192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28019" checksum="6CCD09C2DDB3B953" modified="2016-07-06" version="1">MAFKNEGDVEKMLDLYIYNHMKEINLQDVAEIFAKEANIETSQLVEIEVNLMRRGWLLFWLTLCSRLLNKAGVNAQSSGQQMAFGKEWDKERELAMKNEGDAQKMLELYIYDHMKKKNLLDVASRFAKEANVETSQLGETDENLLLEWWEFFCPAFCSGARVNAQSSGQAMASWENQGAGSTRQTSMVNLQPDMLAEFSTTGGEDNGSSQMPSRGRVDSDGLNHVLSRTHAPAIYLLLMALYVVRTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F3S3</accession>
    <name>A0A166F3S3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0209203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F3S3"/>
    <dbReference type="Gramene" id="KZN07305">
        <property type="protein sequence ID" value="KZN07305"/>
        <property type="gene ID" value="DCAR_008142"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209550"/>
    <dbReference type="OMA" id="YYSAPKQ"/>
    <dbReference type="OrthoDB" id="446132at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008773">
        <property type="entry name" value="PRK11814.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="273"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="544" mass="61050" checksum="516927DBAB215713" modified="2016-07-06" version="1">MSSLLANGISHFPHTESPCFTKGFLPDLIKIKSSKPRTHNSRIFKIRADVDYKPQTLDSDALKGNSDTDQLHKFLKRDYKWGFSEEIDSFSIPKGLSEETIRLISSRKNEPDWMLEFRLKSYQKFCKMTEPSWSDNTYPKIDFQDLCYYSEPKKKPTLNSLDEADPELVKYFEKLGVPLNEQNRLANVAVDAVLDSVSIATTHRKTLEKAGVIFCSISEAIKEYPDLVRKYLGKVVPPEDNFYAALNSAVFSDGSFVYIPKGVRSPMQISTYFRINAMETGQFERTLIVAEDRGFVEYLEGCTAPSYDTNQLHAAVVELYCHEDAEIKYSTVQNWYAGDEDGKGGIFNFVTKRGICAGNGSKISWTQVETGSAITWKYPSVVLEGDNSVGEFYSVALTNNYQQADTGTKMIHKGKNTRSRIISKGISAGHSRNCYRGLVQVQSKADNARNSSQCDSMLIGNNAAANTYPYIQGKNPTARIEHEASTSKIGEDQLFYFQQRGIDYEKAMAAMISGFCKDVFNELPDEFGAEVNQLMSLKLEGSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I5E0</accession>
    <name>A0A166I5E0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0103631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166I5E0"/>
    <dbReference type="Gramene" id="KZN10770">
        <property type="protein sequence ID" value="KZN10770"/>
        <property type="gene ID" value="DCAR_003426"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211186"/>
    <dbReference type="OMA" id="AFINLKC"/>
    <dbReference type="OrthoDB" id="2011998at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF42">
        <property type="entry name" value="OS02G0734300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG84448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="165" mass="18952" checksum="C5601E838E65DD6B" modified="2016-07-06" version="1">MVVALEARNGRHLQRYYKGSRQVVGCIPYRVVENGKDFEVLMISSQRKGKGMLFPKGGWENDESIEDAALRETEEEAGVKGIIEKKLGNWIFKSNGSDVYHEGHMFPLCVEEQLDSWPEKDIRSREWMSASDAKKVCQQWWMKAALDLLVDRFTSPQKQELDAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169W8X3</accession>
    <name>A0A169W8X3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_010575</name>
        <name evidence="6" type="ORF">DCAR_0312023</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169W8X3"/>
    <dbReference type="STRING" id="79200.A0A169W8X3"/>
    <dbReference type="Gramene" id="KZN01821">
        <property type="protein sequence ID" value="KZN01821"/>
        <property type="gene ID" value="DCAR_010575"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211214"/>
    <dbReference type="OMA" id="LHPRIDF"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006393">
        <property type="term" value="P:termination of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF135">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN01821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="57698" checksum="8333350FFEC5E240" modified="2016-07-06" version="1">MAVSTMHIHFLTPPAPFATPPSTLHFSFAPYSWTTFLSVRSFTHRTPLFSIMGFQSPINLIAHCSKKPTIAFIDTGIMLFSVLQDIGIEEKGAKVLLDSHPVLNSMPIESIRARIESLRAIGFDGLALSKLIVKRPDVLTAREMDSFTYYLCNKLKGDVELIQLQRLFNTTEPRFLVGFEEKVSLLLHHGFPEEKLVYVLNNVKINKAFCLKSREEIERMLTYLDRFGGVEFILGRPAVLNYDLDTQIIPRIGFLLELSGGNEDATATVLNKLPFILAYTVEHLKDHVEFLISFAGLTDQEIFRIILVYPNVFSASKDRKLKPRIEFLKECGLSSNDIFKFLIKAPLFVSLSFEGNLAHKLVLLVKIGYANKTKELALAMGAVTRTSYKNMQAVISLFLNYGLTCEDILAMGKKHPQILQYNNKSLDEKMEYLVEDMGREIGELLAFPAFLGYQLDGRIKHRYEVTKHKLGEGMSLNKLLSVSTESFSSKKKKKKLPVKASSKPTKMKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YBL5</accession>
    <name>A0A175YBL5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0623474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBL5"/>
    <dbReference type="Gramene" id="KZM80989">
        <property type="protein sequence ID" value="KZM80989"/>
        <property type="gene ID" value="DCAR_031860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH04068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="14610" checksum="DE04B7EAB33080C9" modified="2016-09-07" version="1">MEYPFFVPDFFKFMTYDDCSSNELLIPQKFCDMQHNALTGNWILSIRNGYKIHVAYDEQSRKLTGLLDLFTDFQLVDGEILLFEYVDNSNFKVYIVGEDGNEIEFPAIVHCSQTSSPVPGKTVSSVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YBS3</accession>
    <name>A0A175YBS3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0934395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBS3"/>
    <dbReference type="Gramene" id="KZM80641">
        <property type="protein sequence ID" value="KZM80641"/>
        <property type="gene ID" value="DCAR_031868"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH14868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="397" mass="43973" checksum="A076D41CF6BFFF1E" modified="2016-09-07" version="1">MNNSNLKISITASPLIDLDRLEQFWRTAKNKGTNEDSSVEFEIDGKEHILTVANINEAFVNTLADNESYRDLANDATLTRFFRKIGYAGPVLKEDLIDRYPTGEMDRKYLRKEWNMLFDAMVKIFSAKTFGWNAIPSYIRKPTHSMVHGYKVNVDKMIMAQLRSAIIRKVQIYPRFVTMFLNGVSGIVANASNTKKCFVLKKNTHITIINSNPHGDMRLHYTKHMSDQVSNLGSSFQDNPVICCLEDEAGPPIIFEILSSTHITSFFSSLTSRAEDRPNLSDVDLPIQAMEEPPSSGPQEPIAQIVMDTTPLNTEFNQGRIQSENETATTLVTLPSATFPIKVAVTVSVAVKDSAGITAPLVVDSIPAVRVSLAVTSIPAGKVHTAVFTQTDSIVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE83</accession>
    <name>A0A175YE83_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_029091</name>
        <name evidence="4" type="ORF">DCAR_0933438</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family. LYRM9 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YE83"/>
    <dbReference type="STRING" id="79200.A0A175YE83"/>
    <dbReference type="Gramene" id="KZM81478">
        <property type="protein sequence ID" value="KZM81478"/>
        <property type="gene ID" value="DCAR_029091"/>
    </dbReference>
    <dbReference type="OMA" id="SLWVLHK"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd20269">
        <property type="entry name" value="Complex1_LYR_LYRM9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045291">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR36758">
        <property type="entry name" value="OS01G0342800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36758:SF1">
        <property type="entry name" value="OS01G0342800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM81478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9541" checksum="3C06B0107356E197" modified="2016-09-07" version="1">MKKVISAYGEVLRLVRRLPEDSRPYYAKYARENFVNYREVDPNDANALQELLARTYNHSLWVLSKYSVEEAAAAKLKNICCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YH29</accession>
    <name>A0A175YH29_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_030037</name>
        <name evidence="9" type="ORF">DCAR_0934898</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YH29"/>
    <dbReference type="STRING" id="79200.A0A175YH29"/>
    <dbReference type="Gramene" id="KZM82468">
        <property type="protein sequence ID" value="KZM82468"/>
        <property type="gene ID" value="DCAR_030037"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200668"/>
    <dbReference type="OMA" id="LMQYGFA"/>
    <dbReference type="OrthoDB" id="6418713at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015605">
        <property type="term" value="F:organophosphate ester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015120">
        <property type="term" value="F:phosphoglycerate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004696">
        <property type="entry name" value="Tpt_PEP_transl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00817">
        <property type="entry name" value="tpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="116"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM82468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH15360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="421" mass="46044" checksum="C54712962E26445C" modified="2016-09-07" version="1">MASLANAMQFSIQRYQFPSGNKLFGARTSSSGLIPCKIQSVSSSLGFSPESFSPVGIPGKALKFSGWDHLLRRRGAVDFQVTKAAAADAGDHEIESVEGISKANPGFFEKYPALLTGFFFFTWYFLNVIFNILNKKVYNYFPYPYFVSVVHLLVGVVYCLISWTVGLPKRAPVNKELLGLLTPVAACHALGHVMSNVSFAAVAVSFTHTIKALEPFFNAAASQFILGQQIPLPLWLSLAPVVLGVSMASLTELSFNWLGFTSAMISNISFTYRSIYSKKAMTGMDSTNVYAYISIIALLFCLPPAILIEGPKLMQYGFQDAIAKVGLYKFLSDLFWIGMFYHLYNQVATNTLERVAPLTHAVGNVLKRVFVIGFSIIVFGNRISTQTGIGTAVAIAGVAIYSLIKANMEEEKRKAAKALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIA9</accession>
    <name>A0A175YIA9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rapid alkalinization factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_030529</name>
        <name evidence="9" type="ORF">DCAR_0935468</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant rapid alkalinization factor (RALF) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YIA9"/>
    <dbReference type="Gramene" id="KZM82960">
        <property type="protein sequence ID" value="KZM82960"/>
        <property type="gene ID" value="DCAR_030529"/>
    </dbReference>
    <dbReference type="OMA" id="NCRQGAQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008801">
        <property type="entry name" value="RALF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136:SF95">
        <property type="entry name" value="PROTEIN RALF-LIKE 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136">
        <property type="entry name" value="RAPID ALKALINIZATION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05498">
        <property type="entry name" value="RALF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044598" description="Rapid alkalinization factor" evidence="7">
        <location>
            <begin position="24"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM82960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH15921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12555" checksum="B23FEB3FB2681FE8" modified="2016-09-07" version="1">MRSSTTLLVICATLLITILSISAVDASAASNSYLSFLPASGTVADFISDESEFDLDSDINRRILATRRRYISYGALSRNTVPCSRRGASYYNCRQGAQANPYNRGCSKITRCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YID6</accession>
    <name>A0A175YID6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_031033</name>
        <name evidence="8" type="ORF">DCAR_0936003</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM83464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YID6"/>
    <dbReference type="STRING" id="79200.A0A175YID6"/>
    <dbReference type="Gramene" id="KZM83464">
        <property type="protein sequence ID" value="KZM83464"/>
        <property type="gene ID" value="DCAR_031033"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201903"/>
    <dbReference type="OMA" id="QEICNYI"/>
    <dbReference type="OrthoDB" id="988630at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11442">
        <property type="entry name" value="bHLH_AtPRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000082">
        <property type="entry name" value="Transcription factor ILI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044293">
        <property type="entry name" value="PRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46446:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR PRE6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23174">
        <property type="entry name" value="bHLH_ILI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="6"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM83464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH16450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="95" mass="10676" checksum="C8C606BF3E63B131" modified="2016-09-07" version="1">MSSRRSRSGQSSGASRISDDQIADLVSKLQQLIPEIRNRRRNSDKVSASKVLQETCNYIRNLHREVDDLSDRLSELLESTDSNSAEAAVIRSLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMF9</accession>
    <name>A0A175YMF9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0830778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
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    <sequence length="241" mass="26080" checksum="97FA5391B33063B7" modified="2016-09-07" version="1">MTPTKQPQSCISVAMGKAVELLPPRALEIFVTTEGKNVPHLTIDFILDIKVQSDVRCWCHAKIKEILNGNGWCYTCCLKCARAVHPVEGISYCLACAEPADPVSQRFIIVVSVDDTTGNTSLTLFNKEAEQLAGVPLANILQEDGDMSKIPAAVNNIIRKVCVFQIKVTPHNTSHGCEKFTVIRVMEEPATEASKVGAHTDVEASKLGAHREAEASKTNAHTTTGREVQAAGNLNKSMKIA</sequence>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="464" mass="49991" checksum="775E161487F2A627" modified="2016-09-07" version="1">MEEEKKNQEILNMSDQNLDPSSSVTPPFSGEEIITPVTYPPPPNIFDMPDIDVEKYDFMEMLRTQNYYFPPTTSIFDLLGAPSPAIGPPQPQQLTAAAVGTSSDMPVLTPVTPNNSSSISSSSNEAVTEDQISIKTGEDDEEHKPEQKNKRLISEPRKKKPKKQREPRFAFMTQSDIDNLDDGYRWRKYGQKAVKNSPFPRNYYRCTSAGCGVKKRVERSSEDPTTVVTTYEGTHTHICPVNAPRGSIGMFPDAYSFRGLGAGAGIGGGGLMSSGIGSSFSGGIGSSFSGGIGEGIGGGMSINAGYRVGGIGSRLVEGGGIGGGSNIGNVSGTGSGSGSISDINSHYAAEQLARYQQRMQQQQQQQQQQRQPYSLYNSIINPSLPSYNFGITNAPAATATTTTTTTSANAAIDSGSFSNRMLQDRHNLLPMTPNQQLTMFRDQGLLQDMVASSQIQSEKKEDKK</sequence>
</entry>
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    <accession>A0AAF0W4L7</accession>
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        </submittedName>
    </protein>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="492"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG83014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="492" mass="54323" checksum="D248DF268704F27B" modified="2024-05-29" version="1">MTRGVATVLKDNDCVAAITSVCWSKRGHHILVAAADKTLTLWDVVKREKVVQVTLQQSALQARLHPGTSTPSICLACPLSSAPMIVDLDTGKETTLPMSLPETNGTTAPPSRNKFSDGSAPFSPTAACFNKYGDLVYVGNSKGEILVIDYKNNQVRGAVPISGGSVIKNIVFNRSGKFLLTNSNDRTIRIYENLLPLKDGVKALDEIIETLKDLTEVEKLKAIGSKCLTLFREFQDSITRVHWKAPCFSGDGEWVVGGSANKGEHKIYIWDRAGRLVKILEGPKEALIDLAWHPVRPIIVSVSLTGLIYIWAKDYIENWSAFAPDFKELEENEEYVEREDEFDLIPDCEKVKQSDVAEDDEIDIMTVEKDTTFSDSDMSQDEVLYLPVDISPDVPEEQDKCIGTGDSNHSGSPLSEDAEQNGQVGKYASSPIDAMDNSDNGGMRLKRKRKPSEKVLEQQAEMSKKPVQKKPLGKLEKKSKPGVDQDSDVSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6H4</accession>
    <name>A0AAF0W6H4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Meiosis-specific protein ASY3-like coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0103085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6H4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037731">
        <property type="entry name" value="ASY3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046845">
        <property type="entry name" value="ASY3-like_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36027">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN ASY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36027:SF1">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN ASY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20435">
        <property type="entry name" value="ASY3-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiosis-specific protein ASY3-like coiled-coil" evidence="2">
        <location>
            <begin position="17"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Meiosis-specific protein ASY3-like coiled-coil" evidence="2">
        <location>
            <begin position="428"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="781" mass="86592" checksum="8B7FAEC3C293F876" modified="2024-05-29" version="1">MKNNKSNLIFILLQGKMSQCRSFGSNYHPSSQPKKISIGIVVDQSMKAKFSKVQQDDAAAAFPVTRNLGSSKVDATEDMHNYEKVKNPTKEKQTDVAVQETSPWASTRSFQQNLPSSQGVCDAHQAPSTLFNQSQRSNNGPQTDDGLQKKVSIGIRAIKGQGDGSSNRVEEVPLTTAQEIKVPGKEVAQEKAENTENKANDTLRMKIWEVLGTVSTPAKTFSRSPTLKTCTDNLEPELNSNKKKCSNVKPRQNSDTIESDSDSLNHTIKRPVTRSLTRKRPTKVQQRKAKITPSSSYKHRKPEKSIFSFEEGRSGRISPAMVGGSSVSKSEKKKSSRVEPRKLQFSKLDNADEICQVTDSSKTKFHVEGSPGFKNGAGGFFGFLKANRDTCQYPIKNRDDTLGINSETVFPKNMDEEDVVDPTVKTIIEPQFDFNTPLLGTNPPTETYICGSPPKSNNEKQEDVYSPVKGVKGVLNMEHIRSFKGFFASTLNSDKKNKETETLDEVVHEESPVEKSLPNLQVNNTVSSPSTSSSEEDGSESSDSSSTEGPSEAEPPEIVTAREPEILFRQTKRCRISEAADAITYSPSTTPPQASGKFHGLPSELNEDNGLARAVSLFAMVLERVKSKMNSAASKRSAEILTSVAMEMQLQLQNAESQIQKDVGKLIGIGKTKRRRLETTFTEQQGKLKDIYARFKNEVNVHLQDCKSTLEGLELQQTEFKDIIEEQNASHGKLLLQAEEAIYTRLNDAEQKVKTIQKSARQKMLQLKYVIAECLKEDAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAU6</accession>
    <name>A0AAF0WAU6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0205753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAU6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032452">
        <property type="term" value="F:histone demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF121">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ706-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="JmjN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="126"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG86542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="54152" checksum="D313A98F697878A4" modified="2024-05-29" version="1">MYYPSREEFEDPLVYLQKVAPEASKYGICKIASPLSSSIPAGMIMMKENRGFNFTTKVQPLRNYTIRDFESFTSKSTAGRYSVSGCLPPDFVEREFWQEMGSSNKRTVEYGINVEGTAFSNNSSDQLGSSKWNLKILPHLLRSTLRLLKHDIPGVSEPMLYIGMLFSTFTWHVENRYLYSINYLHCGAPKTWYGVPGHTTVDFEKVVQQYVYKQEILSTNGEDGAFNLLVEKTTMFSPKILQEHNVPVCKVVQMSGEFVITFLKAYHAGFSHGFNCAEAVNVSTGDWFSFGAAASQRYALLRRMPVVHFEELLCKEAMLLSKHSSDQDISSEDLVIQLHFRPTHFRPQCGRIYNWAPQLSPELLYRPTHFRPSLVGFETIHVYDTSTMGQIYLIQSTWTDVVGRSVVGNDLKTDRFCLFPTDFGVIYYLNLEVCFLFLFMAVLKPKPNRLKPNRCFLKPKPIRRFGCGCGFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBS9</accession>
    <name>A0AAF0WBS9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104307</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBS9"/>
    <dbReference type="KEGG" id="dcr:108204587"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF5">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="141"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG85120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="708" mass="77819" checksum="0A332EAB90A64468" modified="2024-05-29" version="1">MCGMIDLNTVNEDDADTTPSEFDSAPVKSPAVCLELWHACAGPLISLPKKGSCVVYLPQGHLEYVVCDYGDDVASRLPPHVFCRVVDVKLHAEAATDEVFAQVSLIPDSQIELNWKEGKSEVVVEDEDVEIAAKAMTPHMFCKTLTASDTSTHGGFSVPRRAAEDCFPPLDYKQQRPSQELVAKDLHGLEWRFRHIYRGQPRRHLLTTGWSAFVNKKKLVCGDAVLFLRGGDGELRLGIRRAAQVKSGAKVAVAYNEHMNDIASVVNAVSMKSVFNLCYNPRSSSSDFVIPFSKFSKSLANSFSPGIRFKMRIETEDATERRCTGLIVGTGDVDPLRWPGSKWRCLMVRWDDMEATRHNRVSPWEIELSSSVPGASGLLSPSMKRSRIGFTTQPDFPISRDGTGISDFRESSRFQKVLQGQEILGFHASFDGVDARKNHPPDPRGCFPGSMNWDSAVGNGVRSVMGSSEISYDGVNFSESLRLNKVLQGQEILSKRPYGGCPTVNQMLEHNDPRFIEGARAVSGGTEWPSVMQGYSTCMRPSAPLVRKSPMSSVPVFQQPSNPDPKCGAVASRGNLTSSSPYGRNRFEMESQQNLASSGLLNSHKQLGSFNLPLETQASNMGSSNLVSSCKSSCRLFGFLLTEESPAISKESNPSRIQSAYVHKAPHHPRGDEQFNSKPSSMTKIVGGSCTKAKDLYNVRDMLLDIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WD14</accession>
    <name>A0AAF0WD14_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WD14"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="38563" checksum="38371FF5BD4FBA22" modified="2024-05-29" version="1">MATMEQLPYELLYYIILLLVQSPGGAVAFARIISVCRQFLLFAEDEHILKLVNFDLEMEFKNFYRYQNLNSLLIKCSGAGNEAARFLLGKVILVSSSQLRVSEWLKVEWDGHPCDDLKLSDIKSILATDVPGSDHKACSFLTYFLPENLSAGRFSQTRLVHYQLVKLFLSKGSHHDFIKMDIFLKSYIRYFTKCGESSLVFGFIKYLVSRAHHIKAVEKLVKNKELFKDTYRELRDTWEDPSRYSSVRRYLSMTSVALFFLDELDRRCRTVGVDWRQAIYDDDFVEEFWRNGTNNLLDKEYLVFNQVRAGTIHCFEHDLYKFKHVIQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHR1</accession>
    <name>A0AAF0WHR1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0209366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHR1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF208">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="320" mass="36115" checksum="5F2160252267C9A2" modified="2024-05-29" version="1">MGGASLPPGFRFHPTDEELVGYYLKRKVEGLEIELEVIPVIDLYKFEPWELPEKSFLPMRDMEWFFFCPRDRKYPNGYWKATGKDRNIVCQSSAIGYRKTLVFYRGRAPAGDRTDWVMHEYRLSDDVSQDSSSSQGPFALCRIIKKNEQGIKNGDYYGDLTAKQVGCSSSNGASMSNQASGDLVVIPDDMPVQASPLSTPYRTTTMVESESVQVQANPSSLWHSPNFIFDSSLEYSQGQEGLSECFPQNEVSNTMTPWDTFGQFEISPSSSYSNFTEKVELADDLTQFGCMSPYSGHIDYATYFGNGSMPPPEAYDWSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHT5</accession>
    <name>A0AAF0WHT5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208031</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of 2 alpha and 2 beta subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="8"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHT5"/>
    <dbReference type="KEGG" id="dcr:108206569"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="9">
        <location>
            <begin position="70"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG88796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="394" mass="43407" checksum="CBB16A07503DA554" modified="2024-05-29" version="1">MALLRRSPHLLKPLTPPPPAASALSLRHAFSSDTTPITVETSIPFTGHNCEPPSRSVETSAKELMTFFTDMATMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAGITRKDCIITAYRDHCIYYCRGGTLLGTFAELMGRKDGCSNGKGGSMHFYKPNNGFYGGHGIVGAQVPLGVGLAFAQKYNKEDHVSFALYGDGAANQGQLFEALNMAALWDLPAIFICENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDALAVKQACMYAKEHALKNGPLILEMDTYRYHGHSMSDPGSTYRTRDEVSGVRQERDPIERVRKLLLAHDIATEKELKDIEKEKRKEVDDAIAKAKESPMPDPSELYTNVYVKGYGVEACGADRKGVKAVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ62</accession>
    <name>A0AAF0WJ62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0310093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJ62"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF184">
        <property type="entry name" value="OS03G0311250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="16397" checksum="9146AFE9FA22E5F4" modified="2024-05-29" version="1">MQFEKDIGLDPSEGSASDLEEYLGEKPKTFRDFDGFDILEWWRHNSIRFPILSQMAQDVLAFPISTVASESAFSTGGRVLNDFRSSLRPKMVEALICAQDWMRRTIKALCVEEDPEEMKQLDEGIPTSKILKLLIFYCNLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKT2</accession>
    <name>A0AAF0WKT2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metallothionein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0310439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallothionein superfamily. Type 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKT2"/>
    <dbReference type="KEGG" id="dcr:108214579"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000347">
        <property type="entry name" value="Metalthion_15p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33543:SF33">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01439">
        <property type="entry name" value="Metallothio_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG91191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="7897" checksum="67778DBF99E4B497" modified="2024-05-29" version="1">MSCCGGNCGCGSDCKCGNGCSGCKMYPDMSYAEESSNKEALVFGVAPKKEYVFEGSAENGGCKCGSNCSCDPCTCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WL32</accession>
    <name>A0AAF0WL32_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein LHCP TRANSLOCATION DEFECT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0309795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the import of light-harvesting complex proteins (LHCP) and subsequent routing of these proteins to the chloroplast signal recognition particle (SRP) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WL32"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090391">
        <property type="term" value="P:granum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000251">
        <property type="entry name" value="Protein LHCP TRANSLOCATION DEFECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044242">
        <property type="entry name" value="LTD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47317">
        <property type="entry name" value="PROTEIN LHCP TRANSLOCATION DEFECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47317:SF1">
        <property type="entry name" value="PROTEIN LHCP TRANSLOCATION DEFECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG90551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="188" mass="20510" checksum="CC92159CFCB69CF6" modified="2024-05-29" version="1">MGSCSVGGNVVLQLNQHSYAKRQVFRSSSSSSSFVIKSKFVGTIDKRLGWLSLSKIGLLSTTSASASGATCWFKFGKNGVDAEQAGIYGSQSRNDFDRDDVEQYFNYMGMLAVEGTYDKMEALLNQKIHPVDILLILAATEGDKPKIEELLRAGADYTVKDVDGRTALDRASTDEIKDFILGYSVPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNL3</accession>
    <name>A0AAF0WNL3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0311141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNL3"/>
    <dbReference type="KEGG" id="dcr:108211191"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021414">
        <property type="entry name" value="DUF3054"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35283">
        <property type="entry name" value="T12C22.21 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35283:SF3">
        <property type="entry name" value="T12C22.21 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11255">
        <property type="entry name" value="DUF3054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27649" checksum="06D6B7DF17E85884" modified="2024-05-29" version="1">MFLSSQTPVAPVSSSTVHRCSPLKPTLPLPPLFSTRRPPNFSITTRKSPLFLVNSEATSNNNNAPLPKTADSAPPITLVGQDSVPLEGVIQFEKSSFSARLDKWGRVALLAGGDVLALLLFSAIGRFSHGFSVVDVETLRTADPFIAGWVLSAYFLGGYGEDGRGVNGLSQAVIAAAKSWIVGIPLGIIIRGLAAGHVPPTSFILVTMGSTSVLLIGWRTLLYSIFPNDQRKKNDIYKRGSPFELFELLTSLIRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPL7</accession>
    <name>A0AAF0WPL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0311095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPL7"/>
    <dbReference type="KEGG" id="dcr:108210966"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF72">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG91840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="485" mass="54012" checksum="E5B5D193480EEF55" modified="2024-05-29" version="1">MTRVELIFVPAPEVGHLVAAVELAKLLVRRDERISFSILIQKMPYDTGTAAFVEKLKKDDPDRMAFVDIPAPSEATMTELRSLSRTAFHEAFVSHQRTVVRDLVTEILKRSESSKLGGFVLDMFVTPMIEVANDFNVPAYVFFTSSAAFLNVMFYAQDLKDNKNLEISDYKDSDIELSVPGFSNLVPAKGLPSVILDKGGSDMMTSIARRLRKTKAIFVNTVLELEAHAIKSLIDDSNTPLIYHVGPLINSEKGEPTSQKKKSNEDIINWLDRQPCSSVVFLCFGSKGSFDIEQVKEIACALELSGQRFLWSLRRPSENTEKREMPKDYEDYNGVLPEGFLERTSGIGKVIGWAPQVAILSHPAVGGFVSHCGWNSTLESIWCGVPIATWPIYAEQQTNAFQLVKELGLAVEIKLDYRKDVIGDVSPVVTAEEIEVGIRRLMNGEGEMRNKVKTMKDVCRKALDEGGSSYSSVGQFIQEVIDNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVG4</accession>
    <name>A0AAF0WVG4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="4 15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0415583</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4 15"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVG4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014857">
        <property type="entry name" value="Nse1_RING_C4HC3-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08746">
        <property type="entry name" value="zf-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosomes element 1 RING C4HC3-type" evidence="17">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="242"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG96249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="302" mass="34204" checksum="985ED2886F33C576" modified="2024-05-29" version="1">MILLSLMLRMRMRRVGLEESLFLLRVIHIVNSYNDRLFHDYLKKINSCLAYVQFELLVNNVADEQSKLGTKYTVPQIAFYKGIIEAIIQDDTAKGSITNIDALNTRLENQVPNGGGPESQGVSSQVPAAFRNFSLSQKERTLDELVRDKWLCPTSDGRIGLGVRSFLDLRSWFRSNDVPACDVCNEAGVKADPCSNESFNVRIHNYCLTRKFSQRKAERICPGFGTHWNYSVPKSEFVVHEEELQNGNSQNEPPPEPPRRKKQRRGRTEDDTSGSGPSQTCTVKSEMRTTRRSSRLVGASQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WW10</accession>
    <name>A0AAF0WW10_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0416060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WW10"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF94">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="9">
        <location>
            <begin position="106"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG96724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="570" mass="63359" checksum="2D394039EB045E26" modified="2024-05-29" version="1">MATNRQLLKVEPAIWRACAGTSVHIPAVNSRVYYFPQGHSEQSASTPTFSPLVFARPYTLCCVRGVGFFANPETDEVFAKMHLVPVPSDQSVLLRNEQDENDVVSFSKILTPSDANNGGGFSVPRFCADSIFPALNFEADPPVQNLGVRDVHGVLWEFRHIYRGTPRRHLLTTGWSKFVNNKKLVAGDSVVFMRNRRSGEISVGIRRAVKANADCNGRWNYYEDKREREGFSRDVRGRVAVEAVEEAAELAAKGLGFEIVYYPGAGSADFVVKAEKVERSFSVFWTVGMRVKMAVETEDSSRMTWFQGTVSSAVLPDSGPWRGSPWRMLQVTWDEPEALQNVKRVSPWQVENVVATPSLHSEFHPTKKCKLSQDPGLLSAGDGELLFPMPFLSHSLMGHLNPSWMNYNSFPAGIQGARQDQICVSGVPNNILENSQQICSKFFVGDMSPMAETTELNIGSAQVDNLSPDSRSSVHFFGNEQVGKQRVSKCSQKVGISSFQLFGKIIHINNPIEGGLDDVKCTGEDGSKIYREGRVDHPLKRSLTYPFTEFFGTVDVQCQRDSASEDVYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1S4</accession>
    <name>A0AAF0X1S4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0418413</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57783"/>
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            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108216543"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="F:protein-disulfide reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
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    <dbReference type="InterPro" id="IPR036249">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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            <begin position="64"/>
            <end position="76"/>
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    </feature>
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        <location>
            <begin position="77"/>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00012612"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG99066.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="652" mass="75639" checksum="A1D1A8FE7F0D5C39" modified="2024-05-29" version="1">MKKSDISLRSCSTRKRKKIETDDEKGKNKKVKIQTLSHETLEQGGDESAEAIRSDMSLKSCSISERKNNGSKDEKGKKKKSSKKKIKKKKGSYMINLSALRFGRFVRRKKKTTQSCVNDSGPQWVVQKHPKAVREGDRVNLQQLLFTRDRDFLITYNNRRVQAKHLENKVIVLHFVPLVPWSEYWMRFETTILLDVYNALLPKGDFEVVFIGVELDTANAASNCPTPRECFENKFSIMPWIGIPFSDVKSRTDWETVFPLSGFLTRDTPASFVIDHTGMVLQCNANDIFHMYGARAYPFTDKRMECLLKEDAEARNHPSITKLLASSERNHLINKDNQAVPIHNLEDKVVGIYFYEDYPNHALTSEIQKAYEQLAQKNNFEIVLVYVHDSFATCERTSEKSYWKTLKKMPWLALPFQDPVCKKLQRVFDYPLYTCEDEDGPDHSLVIIGPQGKFVERYGADILKNYGIAAYPFTQKGVAKLEAERIKELKLDMFWSRKTTLIQKNGSTVQLSQLEGKRIIVIVEDDQHIPDAKFWRMLRARYLQVKGTGNEFEVVHICKNKERHSYGKNIAPISWLRHPSRHPSHRYGLNVLEIFTRVFRRGSVVGLLAFDRDGRLVRRTPYPSIVKENVDFPFGVMEEEFLRELVDRELEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4C4</accession>
    <name>A0AAF0X4C4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="8">GTP-binding protein OBGC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0520271</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X4C4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF39">
        <property type="entry name" value="GTP-BINDING PROTEIN OBGC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="70"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="269"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH00895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="55294" checksum="3AC213C3C2ECEA23" modified="2024-05-29" version="1">MLSLVSSSSTPSSSSSLSQFHQTCFLPFLPQRLLLNSPSEEFHFSTIKCRLARVKDPPPPSPDNLIKEPHKYFDQVMVTVRAGDGGHGAVLTMPTPKAPSKSHTKYDKGKTRKRVSFKRDFDGSLILPMGGHGGDIVIYVDEGRDTLLEFHKKSRFSAKRGGNVDAMSILTSHLSNGSAGPSLRIPVPPGTVVKHKRGKFLADLARPGDEILVARGGQGGISLLEMPDHKKKKMMTMTSNVMRDESDKVLLLGQPGEEVSLQLILRVVADVGLVGLPNAGKSTLLAATTLAKPDIADYPFTTLMPNLGRLDGDPSLGAGKYSSEATLADLPGLIEGAHLGKGLGRNFLRHLRRTRLLVHVVDAAADDPVNDYRTVKEELRMYNPDYLERPSIVVLNKIDIPEAFNKLPYLKEEIMRIGSDEPSAQVDLNSEDTLQSPSSSNMEQKDNLSSEISEKARRIKEIEEYPRPNAVVGVSVLKGTNINEMLKEIRAALRKCSDPNEVLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X524</accession>
    <name>A0AAF0X524_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0521128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X524"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004332">
        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF171">
        <property type="entry name" value="OS12G0597300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="617"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH01743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="743" mass="84050" checksum="2E2BC0F13C062591" modified="2024-05-29" version="1">MAQDHLISEGKGGSMLVQNEHLGSRDQDSVFGQSCNLVFQQSNESVLSLGHESYDDVSLRLDNERELLVDCELVLSQIKNHGDKHENGNGTDQGKSAKPDVDLNEIGIEECRPQLNAYVENYEFGLLENHGLTILENSEVDKNLDIVHQNDDMGNALHSDMFDLPLVVSPPVLQSRRLVTDPVFELAVGQTFPDVSSCRRVIKETAIALHFEIMTLKSDQTRFTAKCATKGCPWRLHAAKNPEHNCGGITHLGHQQASVQWIANSLEQIIKENPNYKPKDIVEKINRDHGITLSYKQAWRGKERVMAILQESTEGDYRLLPQYCDQIRRTNPGSIATVYVNPADSSFQRLFVSYHALIYGFLNGCRPLLCLDRIVPNSKHLCTLLYATGYDGNGELFPLAFGVVEEENDENWIWFLSELHELMKAKTECIPELTILSNMKRGIVDGVEQSFPTAFHGYCMRFLIESFKKEFNHKKLANLFLEAANALTIFEYESIMLEIGATSQEAASWIQQLSPQLWANAYFKGTKIGQLTANIVESLDSWIVNACKLPIIQMMECIRRQLMTWFCERREASTQWASILVPSAEKLVSGAIECARTYQVFRANEAEYEVLSAELPFIVDIQKCWCTCQGWQLLGLPCSHAAAALLFCNQTVYRFTESCYTVAAYRNAYSETIHPVPDKRQWNQADENSVPNIVINPPKSIPPLSKPRKRRVRSADLGRVKRTVHCSRCYQPGHFRSTCSTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XA62</accession>
    <name>A0AAF0XA62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0623450</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XA62"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF202">
        <property type="entry name" value="SENESCENCE-INDUCED RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042195301" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="586"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOH04045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="888" mass="99114" checksum="4D220D499D95C9DE" modified="2024-05-29" version="1">MLSKSYLLFELVLAYFLNPIPLVHAQDDPPGFLSIDCGLPEGSDYKETYTGLNYKSDANLIDSGESKSLLPSLISDSYETYLGNVRSFPKGKKNCYTIQPSGGKGSKYLIRATFMYGNYDSLNQFPIFDLNLGPDTWSKVTIEANLSVERKEIIHVLSSDYIHVCLVNKGTGTPFISALELRPLSSTNSMYTIESRSLQTLIHADCDSKVTNTDTRYKGDIYDRTWYHFNVSTNKEVNTSLDINNANDYRVPTNVLRTASIPENATDSLQFEWTTNNASDEFYMYMHFAEVEKLQANQYREFNIYINGKRWNKQLVVPEYLRATYYYPLSPLTGNTEYIVTLNKTASSTLPPIINAFEIYTGVIFSKSGTNETDVAAILNIKSTYKVTRDWQGDPCEPGDFLWDGLKCNYDTSDSARIISLNLSMTGLTGEIVPSIANLTELKTLDLSRNNLSGQVPDFLSQLALSVLNLTGNNFTGPIPAQLLENERQGILSLSFDGQEKGNDTSKKNCEQEPCKKKGNKSNAAIVGAVVGSVLLLAAILIGIWIWRSRRASQVRKLRNQAQVNNGDIEKKNRQYTYSEVLNITRNFQKVLGKGGFGTVYHGYVGDLEVAVKMLSPSSTQGHKEFQAEASLLLNVHHKNLTTLVGYCNEGTNMGIIYEYMANRSLDKHLKGNSFGILSWQFRLQIALDAAQGLEYLHHGCKPAIIHRDVKTSNILLNEQFQAKLADFGLSRAYSAEGGTHVSTVVAGTPGYLDPDYYTSNRLTEKSDVFSFGVVLLEMITGRPAILTEDRTHIAQWVDSVVQNGDVKQVVDPRFRGKYDVNSVWKAVELAMTCASRISSRRPTMNKVVMDLNECLAIEIGKHDSDLYSSNGMVSMTDMESALAPRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XC92</accession>
    <name>A0AAF0XC92_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF223 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XC92"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd04481">
        <property type="entry name" value="RPA1_DBD_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36191" checksum="7EC69A99937E375C" modified="2024-05-29" version="1">MFDHLADLEKSRTDWKIKAWVTRLWPTNNAESGVVKGFNLILLDNDQFNLITQRHKLCSMCTVDVMGMIEEWEPASRVPTRYGERETLRFKLSDGRVVLGVCLWGELAVTVSNEYESEKKKPVVAIIASSKLTTFRQELQIGALPSTRIYLNLNIEAVEVFRKRFQLLVLADDNSFASTVVLTDRVVKRLAKTTVTNLMNSSKEAPNSEMPPVLRNIVGKTVTVKISLSKSNVAGDSNIYKVVDLCEGSVSGKKVAEYSPITKFPSFEQSQTDDYVVCLETPTSSDSVSKKIKMVKCFCIILMHMIVEQIEIIFTMMVFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDC0</accession>
    <name>A0AAF0XDC0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0624334</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Required for the correct development of pollen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDC0"/>
    <dbReference type="KEGG" id="dcr:108193062"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000035">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF884">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="23"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH04922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="371" mass="42165" checksum="ED897A6968A1194E" modified="2024-05-29" version="1">MGATGSKLEKALGDHFPEGERYFGLENFGNTCYCNSVLQALYFCVPFRDQLLEYYANNKSSSDAEENLLTCLAELFSQIGSQKKKTGVIAPKRFVQRVKKQNELFRGYAHQDAHEFLNFLLNELVDIVEKEHQAAKDPLGTSPPDKIANGVKTSQANGVKKEPLVTWVHENFQGILTNETRCLRCETVTARDETFLDLSLDIEQNSSITSCLKNFSSTETLNAEDKFFCDKCCSLQEAQKRMKIKKTPQILVIHLKRFKYMEQLGRYKKLSYRVVFPLELKLNNTVENSDSEYSLFAVVVHVGSGPNHGHYVSLVKSHNHWLFFDDENVEMVDESAVQTSFGSAQEYSSNTDHGYILFYERAPSNGLIKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDM4</accession>
    <name>A0AAF0XDM4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0625298</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDM4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000433">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF60">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041989991" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="22"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="626"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH05875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="976" mass="108455" checksum="CA8326B8BC796B49" modified="2024-05-29" version="1">MGFLHSTFVLLVLYFMLLSDAQLLPAQEAHDISRLNCASGVLEGFNYQACRISLISAEMKGLNLTGELPAEFSNLTNLLVIDLSRNYINGSIPSAFGQMHLTNLSLVGNRIRGSIPPEIGNIDTLEELVLENNQLEGPLPAQLGSMIRLKRLLLSGNNFSGKIPETYGNLKNLTDFRVDGNGLSGKIPEFIGKWTNITRLDLQGTGMSGPIPLAISLLNNLEYLRISDLSGTNSSTIPDLKDMKSIKTLTLRNCLMSGEIPEYIGKLITLKSLDLSFNKLTGPIKDALEPLHSKMNFLYLNNNSLTGAVPKWISNSNDNYDASYNNFTQTPGYNCQTSRVNLISSHSSTTDNSGLDWCLKKDLPCSRKPRYHSLFINCGGNKTQWERNEYEEDTNEEGASYFYSDSSEKWAYSSAGEFVGRKGADYLAINDNITEEIYRTARLAPSSLRYYGLCLQKGSYKVRLHFAEIQYSGDNTFSSIGRRIFDVSIQGHLRKKNFNIAEDAKGFGLPTTLEYNVEVDDGTLEIHLYWAGKGTNAIPDKGVYGPLISAITVTPNFKVDTGRLSTGAIAGIVVCSISGVILILLLLWRKGFLGGKDIEDKELRGAIELQTGYFSLRHIKAATNNFDPANKIGEGGFGPVYKGVLSDNAVIAVKQLSSKSKQGNREFINEIGMISALQHPNLVKLFGCCIEGNQLLLVYEYLENNSLARALFGHEEQKLNLDWPTRKKIFLQIARGLAYLHEESRLKIVHRDIKATNVLLDKDLNAKISDFGLAKLDEEENTHISTRIAGTIGYMAPEYAMRGYLTDKADVYSFGIVALEIVSGKSNTNYRPKQEFVYLLDWAYVLQEQGKLLDLVDPILGSNYSKEEALRMLNMALLCTNQSFSLRPSMSTIVSMTEGKTKVEAPMRTESEDDMRFKAFEKGAHDSQSHSYTFSQESRERSISVDGPWADSSVSFQSKDASTSKLLPDLYDVNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XE95</accession>
    <name>A0AAF0XE95_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May promote appropriate targeting of ribosome-nascent polypeptide complexes.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XE95"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000006">
        <property type="entry name" value="Putative nascent polypeptide-associated complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="3">
        <location>
            <begin position="5"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="4">
        <location>
            <begin position="74"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH06328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12633" checksum="2D773174268ECA3D" modified="2024-05-29" version="1">MFFQILFVISKPDVFKSPHSDTYVMFEEAKIEDLSSQLQTQAAEQFKVPNLSHVMPKPEPSVTAQEEEDVNETGVEPKDIELVMTQAGVLRPKAVKALKAANGDIVSAIMELTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFS9</accession>
    <name>A0AAF0XFS9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0625680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFS9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000005">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000050">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000002">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="944"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="9">
        <location>
            <begin position="33"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="177"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH06257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="967" mass="112776" checksum="099CEFE5698F29B8" modified="2024-05-29" version="1">MGRGKWGTRSNPKRGKTIDITVENRELDDGPHAYRFSWQLRNFSRLNDKVYSTHFSCGGFNWRVLIFPNGNNVDCLSMYLDVADALSLPYGWNTRAQFSMSVINHIDPKYSVRKDAEHIFHARESDWGFTSFMQLSELYDPNKGFLLRDTCFIEADVAVRKVIDYRGYDSKKMTGYVGLKNQGATCYMNSLLQFLYHIPYFRKAVYHMPTTENEIPSGSIPLALQTLFFKLQYHDTSVGTKELTKSFGWETCDSLMQHDVQELNRVLSEKLEQKMKGTAVEGTIQKLFEGHHVNYIECINVDYKSLRKESFHDIQLDVKGCRDVYASFDKYVEVEHLGGDNKYHAENHGLQDAKRGVLFSNFPPVLQLQLKRFEYDFMRDTMVKINDRYEFPLELDLDIEDGKYLSPDADRSIRNLYTLHSVLVHSGGLHGGHYYAYIRPTLSEQWFKFDDERVTKENLDSALEEQYGGDEEFTPTNPGFNNAPFKFTKCSNAYMLVYIRESDKEKIICDVDEEEIAEHLRVIFREMNCSSDFVNIIVTHFDVARDEDLLQQIGKDIHFDLVDFEKVRHFRIQKQIPFMKFKEEVAKEFGVPVQLQRYWIWAKRQNHTYRPNRCLTPREEAQMVGQLREISNKGNNAEMKLFLEVLPGPEQCPIPPPGGEKEDILVFFKLYDPKKEELRYVGNLFVNCAGKPIDILRNINELAGFDPDEEIDLYEEVKFEPCIMCERLDKGSSFRSSQIEDGDIICFQKHLQPHSMEEYRYSDVPSFLEYVKNRQIVHFRPLESPKDDNLCLELSKMNTYDDVVGSLALKLGLDDPSKIRLTPHNCISQQPKPHPIRYRAAENLLDMLAFRDQISDILYYEVLDIPLPELQSLKTLKVAFLRSPEEEVVVHNIRLPKKSTVGDLLDEIKTKVELSHPNAELRLLELLHHKIYKVRTTQIRFKVVIMYLYILVYILISAFLHILLYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XGL6</accession>
    <name>A0AAF0XGL6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucan endo-1,3-beta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0625856</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XGL6"/>
    <dbReference type="KEGG" id="dcr:108225587"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042046329" description="Glucan endo-1,3-beta-D-glucosidase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH06429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40381" checksum="A0315A87C8C49865" modified="2024-05-29" version="1">MSPKVTTLLLLLSSLFAFLQATSGAGPQDVGVCYGSFGNNQPSAQEAVPLAQSMKIRRMRLYSPDRNALQALRNSGIEVILGVPNDHLHWVASDQRNAHEWVQGNVIDYSNVNFRFIAVGNGITPFHSQNARFAQFLLPAMQNIRNALSAFGLQNRIRVSTAIDQSEVLAQSFPPSSGNFRPEVRQFIQPIIQFLVKTGNTPLLVNLHPYFSYIHNKQDIPLQINKGKGNRDARLDYALMRSSNMLVQDAWLRYTNAFDAMVDSVHSAMEKVGGNSLDIVVSEVGWPTAGGPAASNQNAETHNNNLIRHVRNSGTPKRPKKRIETYIFSMFDEDRKSEENLRHWGIFWNNKQAKYRINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XLX4</accession>
    <name>A0AAF0XLX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0728909</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XLX4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF98">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH09452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="42889" checksum="FE0F2459C4ED25AB" modified="2024-05-29" version="1">MHFQGLVFLNLGYNNFSGRIPTSIGYLVSLQTLILRSNNLYGELPSSLRNCSILGFADFGLNKLSGKVPSWIGKDLPQLYALILKSNRFHGSMPHEICYLSNLHFLDMSINTISGTIPLCFYNLTAMIRKGSEVAEHTYSSVDFTDIIASPPEGAPTTNFTYFDNVLARWKGQEFQYGSNFAYLKMIDLSANNLTGEIPVGVTKLLELKGLNLSGNRFNGKVPLKIGQLKGLECLDLSANKFSGEIPQSMSGLYFLAFLNLSNNNFSGRIPSGTQLQGFNTSTYEGNTGLCGKPLINICPGNEPADNDGRSSNEFEVEGDDSEYERWLYISVVLGFSTTFWGMIGTLVLNRRWRYSYFLFLENLKERIYVMMVVRIGRLQRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XP57</accession>
    <name>A0AAF0XP57_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0729857</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XP57"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000446">
        <property type="entry name" value="protein ANTHESIS POMOTING FACTOR 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH10389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="37993" checksum="7E5D5D2E15D17DE3" modified="2024-05-29" version="1">MYNCAVVCIITGGVQSEREDKVSLELNEEILQSMEVGMAFRDYNGRISSLDFHKTSNFLVTASDDESIRLYDISNATCLKTINSKKYGVDLVCFTSHPTTVIYSSKNGWDESLRLLSLHDNKYLRYFKGHHDRVVSLSLCSRNDHFISGSLDRTVLLWDQRADKCQGLLRVQGRPATAYDDQGLVFAIAFGGYIRMFDVRNYEKGPFEIFSVGGDMSDANAVKFSNDGRLMLLTTMDGHIHVLDSFRGTLLNTFNVKPVSSTSTLEASFSPEGSYVISGSGDGSVFAWSVRSGKEAASWMSSETEPPVIKWAPGSLMFVTGSSELSFWIPDLSKLATYVGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR53</accession>
    <name>A0AAF0XR53_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphosulfolactate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0831254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XR53"/>
    <dbReference type="KEGG" id="dcr:108197484"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48413">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48413:SF1">
        <property type="entry name" value="PROTEIN HEAT-STRESS-ASSOCIATED 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="(2r)-phospho-3-sulfolactate synthase ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32653" checksum="D68823B0E2CAAC8F" modified="2024-05-29" version="1">MYRWKTRFHEEEDRPEKPRRYGVTEMRAPNYTIFTRDLLQETFESMGEFVDGLKFTGGSNSMMPKSFIKDVTDMARKHNVYVSTGDWAEHLLRKGPSGFKEYLEECKQLGFDTVELNMGSFGFPEETLLRFVRLIKNAGLKAKPQFSVKFEKSDIPKAGNRAYGSYVVPTPQNSEMIEDMDLMITRAERCLDAGADMIMIDADDVCKYVDTIRSDIIAKVIGRLGLEKIMFETSNAKASEWFINQYGPRVNLFVDQGQVLNLECLRGQNIGNISRSVFGRSSFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR63</accession>
    <name>A0AAF0XR63_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0831269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XR63"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF37">
        <property type="entry name" value="OS05G0311600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH11777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="223" mass="25434" checksum="3B35E10168E9B207" modified="2024-05-29" version="1">MDKFVIKTKRPRSPGSVNKNSGAKSGAKTDSGSNASVPNPIPNAPVEERASTEFNSEELISDPGLRIPIAEYNVNIRDQVRRAYIAKGPFQITDYNFPKKQLNKEMRSFQANWFKEFDWLEYSVAKDEAYCLWCYLFKPDREENTGKNAFTTAGFNNWKKALVVFRAHVGSVGSSHNEATRHCQAFKNQRQSISHIFSAQGYEIEVDYRKRLTAVLNVIRLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSH0</accession>
    <name>A0AAF0XSH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription elongation factor SPT4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0832784</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">May regulate transcription elongation by RNA polymerase II. May enhance transcriptional pausing at sites proximal to the promoter, which may in turn facilitate the assembly of an elongation competent RNA polymerase II complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSH0"/>
    <dbReference type="KEGG" id="dcr:108197722"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000002">
        <property type="entry name" value="Transcription elongation factor SPT4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="10">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH13275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="116" mass="13293" checksum="E2AA755137A42049" modified="2024-05-29" version="1">MAHAAAQIPTSFGHELRACLRCKLVKTYDQFRESGCENCPFFKMDEDNERVVDCTTPNFTGVISVMDPPRSWAARWLRIGRFRPGCYTLAVSESLPEDLQNLCEDEHVPYNPPKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSP2</accession>
    <name>A0AAF0XSP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0832108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029472">
        <property type="entry name" value="Copia-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222:SF100">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34222">
        <property type="entry name" value="GAG_PRE-INTEGRS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14244">
        <property type="entry name" value="Retrotran_gag_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Retrotransposon Copia-like N-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH12602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="415" mass="47272" checksum="39E41F00A07F1A7D" modified="2024-05-29" version="1">MLMCMYQTEQALTSINTRVLSSLPLISSIYTYKHSHTKENSSPIQTILNGSNYITWSQAMRSYLKGKRLWRYVNGDFTIPTKEKDETDSKFKDRIEEWDVKNHQAITFFRNSSVSKINLQFGNYDTAKEIWDLLASRYTTSDLSHQYQIMNFLSQIQSFWDQLTLSEPKWLHTEDATLYHTYRDQQRLIQFLMALTQDFEPVRAALLHRVPLPSLESSVTELISEETRLGIGKINSMTDTVLAVPPVSTSDKPFCRFCRQSGHVTKDCVALRNHTCQHCHQKGHYSIQFCKKKPSFHANKFTRSSAAATSESTSENAPTTSTSELESLLKQVLSKTSSPSTAFSVTSGTSYVILVLILSSPLLVVMYRILRQDRLLGQGVDAEGCLSSLPFIFQNHHLLIWLLLFPLQPAYGILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVH0</accession>
    <name>A0AAF0XVH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0934566</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Alfin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVH0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF98">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH15033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="271" mass="30461" checksum="9605FDA65A78E172" modified="2024-05-29" version="1">MDAGEDASIKMSVNDVFKDFRGRRKGLIKAMTTDFDKFYEDCDPGKENLSLYGYPDGSWEVKLPVDEVPPEYPEPVVGINFSKEGMCQSNWLSLVAIHSDAWLISTAMYLGARFSFDKNDRMRLFEKINGLPNVFEVVIGNFSEQLSTDPSNVKEAFTAKQPEQLAANQSNEKTNISGKMTQSFGLQPKRVKMSPPPKADVDSDEEELADELDTSKCGACGEQDDAEGFWIFCDVCEKWFHGECVKITRAKAKQIGHYKCPGCSIRRARVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y261</accession>
    <name>A0AAF0Y261_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0935765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
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        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
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    <comment type="similarity">
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    </dbReference>
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    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
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            <dbReference type="EMBL" id="WOG81059.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="50" mass="5720" checksum="06AA7739187B0303" modified="2024-05-29" version="1">MEEPTRKGCNQRKVLTLSTTLFSDDVPWAKGDKWVGSFPVPIYTIFCLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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        <text evidence="1">Belongs to the plant acyltransferase family.</text>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG81899.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="218" mass="24595" checksum="8E9C254D9A9EABA3" modified="2024-05-29" version="1">MALDIEVLSKQDIKPSVPTPQHLKKYSISFLDHIAPRVFIPLIFYYTAIDKDKNNLKFDHLKKSLSEALTKFYPLASRFIDNAHVYCNDDGAHYVEARVDCNLKDIIRDPIPNELIKLLPLEVDDAAVDESLLGAVISHKLADGLSCVNFMTTWAPIARGEPNVPSPILDLVKLFPPLDISGFEDRIGILKENIICKRFVFDATKDMTQPTRFEAVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AEX4</accession>
    <name>A0AAF1AEX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">50S ribosomal protein L21, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0101070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AEX4"/>
    <dbReference type="KEGG" id="dcr:108202376"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_bL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_bL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_bL21_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG81914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="23856" checksum="0D5C221E03C59C82" modified="2024-05-29" version="1">MALTLSPSSLSSPSSLSSPKQNPLSLSLSTPTCNFLSHNFSSLTLSTSFKPTFSFVTKFSQTDQSVVVEAESGSENGSEPEPEEAQVVESQVVESSSEEPKKEDVFAVVMIGGRQYIVFPGRFLYTQRLKGANVNDKVILNKVLLVGTKTSTYIGKPVVPNAAVHAVVEEQGLNDKVIVFKYKKKKNYRRNIGHRQPNTRIRIMGITGYEDSPAVTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AL06</accession>
    <name>A0AAF1AL06_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AL06"/>
    <dbReference type="KEGG" id="dcr:108207358"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35296">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35296:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13946" checksum="F0FFB3E2E57E048F" modified="2024-05-29" version="1">MECLVLPVSLLRRQCIRTRLGYRELKGGGFGTSDDPVRVVVGKERREFWVDPFVLEENPFRVLIELVNRNEGRGSKKRVLFVDVDAILFEHMLWLMHNDSSSLFQLNLKEVVDFYAQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALG2</accession>
    <name>A0AAF1ALG2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-catenin-like protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103603</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALG2"/>
    <dbReference type="KEGG" id="dcr:108223009"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000245">
        <property type="entry name" value="Beta-catenin-like protein 1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="7">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="520" mass="59359" checksum="4E55BF6AAB7CD21E" modified="2024-05-29" version="1">MERANNKRSREAAGINAAADDNIDLSLLEAVEKSQNSIQVLDVKTLKRLVLAFERKFRDNIAARLKYPDNPEKFADSELELHDEIEKLKILAGGPELYPELVNLNTIPSILSLLNHDNTDIALDVVGLIQDLTDEDVLEDNDENAQVLVDCLIENNVLELLVQNLLRLNENDSDEMAGAYNTLATIENLIEVRPAVAEMVCERTKLLRWLLGKIKVREFDGNKQYASEILAILLQTSAGNQKRLGGINGVDVVLQSVAMYKSRDPKSSDEEEMVENLFDCLCCLLMPLENKEKFLKAEGVELMIIIMNQKKLCYGSAIRALDFAMTNYPPACERFVDVMGLKTAFPAFMGKVPMNKNKKRRNKEELEERLISLVASLFGGISRGSRRERLLSKFVENEFEKIDRLMELYMRYSNRVKAETERLNNHELDDLEMDEEEIYNRKLESGLYSLQLIAVILGHLWTSDHSGIKARIELLLKQQKLTRKDVRDILQEYHDNIGDLDGPEEKERVQTKIEKFISAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALV7</accession>
    <name>A0AAF1ALV7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0104070</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALV7"/>
    <dbReference type="KEGG" id="dcr:108204907"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="16">
        <location>
            <begin position="87"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG84885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="237" mass="27566" checksum="76266957BCDDC8F0" modified="2024-05-29" version="1">MVAKDYTVDLNKPLVAQVGHLGESYQEWVHKPIVTKEGPRFFENDVMEFLTLNDWWVIPLIWFPVVCYFEFKSFRMGHDFPQIALMVVCGILIWTLMEYTLHRFLFHIKTKGYWTNTLHYLLHGCHHKHPQDALRLVFPPAAAAILLVPLYNLVKLAATPSTTPGLVGGILFGYVCYDMTHYYLHHGQPTSKVPRYLKRYHLNHHFRIQTMGFGITSSFWDRVFGTLPETKSAAKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALZ9</accession>
    <name>A0AAF1ALZ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0207343</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALZ9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF1">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="129"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="560"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG88109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="671" mass="75214" checksum="17D442BEFEAC9764" modified="2024-05-29" version="1">MANLGCISQPHLLSQGNNGGMEDMYTELWKACAGPLVDIPRNGERVYYFPQGHMEQLEASTNQELNQSIPLFELPNKILCRVVHVQLKAEQETDEVYAQITLLPEPRQNEPISPDSCHPEPPRTAVHSFCKVLTASDTSTHGGFSVLRKHANECLPPLDMTQATPTQELVAKDLHGTEWRFKHIFRGQPRRHLLTTGWSTFVTSKRLVAGDSFVFLRGENKELRVGVRRHAQQQSSMPSSVISSQSMHLGVLATASHAVATQTLFVVFYKPRTSQFIIGLNKYLEAVKNGYTVGVRFKMRFEGEESPERRFTGTIVGVEDVSSQWKCSKWRSLKVQWDEPASIARPERVSPWEIEPFVASVPPSLSQTAPVKNKRPRPPSEISALEPTSTAVSAAWNPSHDSSQLNVSLEGQRTDSNVYRQPMQSAKYSNLTTESSHVRAPWSKISDENEDSKSASAWSVQRSFTNQNPTYQYNEQLPLPVEEKKSDAVTTCRLFGFDLKSSTDAAKKVTPLTPVNVFIKAADGGDSEEKSELSKDSKMKHQGLQVSPKEVQSKQNCSTRSHTKVQMQGIAVGRAVDLSVLRGYDELISELEEMFDIKGQLYPRDQWEIVFTDDEGDMMLMGDDPWQEFCDMVKRILICSSQDVKKMRVGMKLPISSTDHEVSGFSLDSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMG7</accession>
    <name>A0AAF1AMG7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0105100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMG7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG85907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="100" mass="11998" checksum="3098119544826848" modified="2024-05-29" version="1">MGSTDKDIKVYEWAVQKVTYSVDMWLHYCVFAINTYGDLDTIKRLFGRGLVYVGSDFLSYTMWDKYIEYEYTHQEWSRLAMIYTRILENPNQQLDSYFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1APV9</accession>
    <name>A0AAF1APV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1APV9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21546">
        <property type="entry name" value="SPOC_FPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF2">
        <property type="entry name" value="NUCLEIC ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="559"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="891"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="891"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG90763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1101" mass="121881" checksum="9C9AF38DD01CAF54" modified="2024-05-29" version="1">MVSADQPLKKRKLYESHNSPPQSLSDDEIQRRRRNQEEIRNVYEHYKLLKQCISNKNPNFMPKLEESYLALLTASRGCTSVQRIVAEFIPRYASFCPTALEAATKAVINIHNCSLAVINRGEDGDGVACRTATLCISGLVEICQAAQSEAPTSSVIQRICSSVFIDVLSFFVSTFNGIDLFHIVDKGVLKMQDSTSLFSDFKQKISAEDDNEVAKLSKLRALSLIWILFCCPKNALAASFELCDSVATEELHNHGLYFLRQITSEFEPNDVANHVDSKKGGIVLSTDSDNPMHETEEITHHGLVSHDNHKSDGPPLILKKCLLGLVVSKNLPLRKWMFLRYKKFCTSASSQVASKITSLLEEVFQSFAEQVKAADDQLNSIEDNSIASKNFSAQYLGLKRQNSEICRRDGPYVNNLTEKLLDQKLTDSRPLISPETPMRVTVGSNVDVGGPRSMDFDTSDAGEVLRPRSSTPRDLLNNQMHSPIRRKSFDMRSNSFEGTSYFIIVKTLPLKSSAGSIGHLQLPFDSPQPHMPLPSPSTSHGSWFSDGDPAAMDIFSASRRLWLGSLGPDASEALVRFQFEKFGPTEQFLYHPYKGFASVEYKNLMDAVRARGYMRGCAPWGAPLHVKFLDIGLGTRGAINGVAIGSCCHVYVGNVPTQWAKDEILHEIKKVVYKGPLMLTDLRSEMAVLMEFGNPEEAANVMAHLRQWRKESCKYLLPSNVGPANARMHLESSRHGPITPAYFRSKSGNSFTESPHAQTVLESPSESYRTKIRGGLVANEQMWTYNNPKTEIYPAQGTHSFMTTASQGPPLPPQPNYASSSMRPYFPLSNCWEARSLSNPHPPNPASVGLVPTIIHTPVVPPPFLPPSVTPLTQMPGSSVQQFDQTFFRPAVPPPLINVPPQPELAPPLPPPPSSPPPLPQSLPPVVPPPPSSPPPLPPREYSNVVSSGPCIAHSWQGTLSKSGVHYCTVNAQRLHSDMCKYSNASSEPAEWPAKLDMTKRTDFRHVKTTFSSTPAHKREVCQLLPSAAGDYKGFQDFISYLKQRECAGVIKIPAARPMWARLLFILPYSPEVVSMLSISPTSTDCLVALVLPKETNFEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATP2</accession>
    <name>A0AAF1ATP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">biotin carboxylase</fullName>
            <ecNumber evidence="3">6.3.4.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0311962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051602">
        <property type="entry name" value="ACC_Biotin_Carboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48095:SF2">
        <property type="entry name" value="BIOTIN CARBOXYLASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48095">
        <property type="entry name" value="PYRUVATE CARBOXYLASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl phosphate synthase ATP-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="106" mass="11533" checksum="EA6B0D638473CC74" modified="2024-05-29" version="1">MKNAGFTIVPGSDGLLQNTKEAIRLAREIGYPVMIKAEILVFATAGGGGRGMRLAKERDKFGKLLQAVESEASAAFGNDGVYLEKYIHNPMHIEVQNTLHILYLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AV30</accession>
    <name>A0AAF1AV30_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein L34Ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0312596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AV30"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012870">
        <property type="entry name" value="DUF1666"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46741">
        <property type="entry name" value="OS09G0413600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46741:SF2">
        <property type="entry name" value="RIBOSOMAL PROTEIN L34AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07891">
        <property type="entry name" value="DUF1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="94154" checksum="E26B60386B09E103" modified="2024-05-29" version="1">MSVRRLFISCSCFSSRNTGLVNECLHYKMSSFLAIGWFSLSTLLLPLFGIVFSYIFRKHSSMNYSSSFNYVKEENNIDDSENDGLFKDGNSDFCLRFKFPTYEEFSRSEYDSCNLISFEEKPSLSSRKYEFTPTESVSGFVDEMEPSRYKVERVNAEMKSCSFGNGEVKEDELWLDRVISSLYVEAESDHKEGYKVKGDELLLDRVISPLHVEADSDEKEIHKGSTDGLSVEHVIKDNDAPEVMGDEENLISEKDKSDACDEEEEPSSMDNGFLEAFSSNDVHTFKSHLVDSCNNGFLSDGEFGGTFVLDHMMDLDEHKEESDMKMKELDKENQESEDLGEEDSDIVKELRIFEEESLQPSHKFKYNFLSEKHFGEQLESSGRRDHNYIKGSGKLASPGLKNDSVMDSDQDTNKLETLWEHQDLIEQLKMELKKVRATGLPTILEESESPKIIEDLKPWKFDDKFHHDNPMGELHKFYKSFRERMRKFDILNYQKMYAIGFLQLKDPHQSISVEKSSAVDITTSLWENLYMYKSKKHETDPTKKFIKELQSDLELVYVGQMCLSWEILHWQYEVALDLWESDPRGIHRYNEVAGEFQQFQVLIQRFLEDESIEGSRVQNYVKHRCVLRNLLQVPIIRGKCNLDNITDISKDKKKSRTSKRDEYSITSDMLVEIVEESIRIYWRFIRADKNCSTAILKSRKGQTELQDPADSQTFREVQKDFKKKDRMLKDQIRSGNCILKKLKKCREDDAEDQVLIFFSQVDMKLVSRVLNMQRLTADQLAWCRNKLNTISFVNRKIHVEPSFCLFPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWK6</accession>
    <name>A0AAF1AWK6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0314403</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AWK6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000023">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000031">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="82"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="277"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG95101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="428" mass="48287" checksum="2F18165E7CF088FC" modified="2024-05-29" version="1">MDSSSCPSNAIPVVSTFTSPFETCDDQKKPLSLWPGMYHSPVTNALWEARSSIFERLLDFSVDAPPQSQLLTRTPSQSRTAILYNFSSDYILREQYRDPWNEVRIGKLLEDLDALAGTISVKHCSDDDHMTRPLLLVTASVDKMVLKKSISVDIDLKISGAVIWVGRSSIEIQLEVTQLTNGSTDTFNSVALMANFIFVARDSKTGKAAPVNRLSPETKEETLLYEEAEARNELRKQKRGGDKRDIENGNRLEALLGEGRIFSDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMHRAFELAFSTAYVFAGMMPCFLEVDHVDFLRPVDVGDFLRFKSCVLYTEYENADKPLINIEVVAHVTKPELRSSEVSNKFYFTFTVCTEAKAKSNGFKIRKVVPATEEEARTILERMDAEVMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZ60</accession>
    <name>A0AAF1AZ60_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417307</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Water channel required to facilitate the transport of water across cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family. SIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AZ60"/>
    <dbReference type="KEGG" id="dcr:108215818"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000043">
        <property type="entry name" value="Aquaporin SIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044222">
        <property type="entry name" value="SIP1-1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46739">
        <property type="entry name" value="AQUAPORIN SIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46739:SF3">
        <property type="entry name" value="AQUAPORIN SIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG97966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="236" mass="25323" checksum="CB6EC070D58719D1" modified="2024-05-29" version="1">MGAIKSAICDGVLTFLWVFCVSTVGPLTYVIATALSIKGIFSLAITTVLVFLLLFIFGFIGEILGGATFNPTATAAFYAAGVRGVSLFSAALRFPAQAAGAVGGALAIKEVMPEQYKHMLKGPSLKVDLHTGAIAETVLTFTITFAVLFIVLRGPRSPIVKTWLLAMSTVTLIVLGASYTGPSMNPANAFGWAYVNNRHNTWDHLYVYWICPFVGAILSAWIFRAIFPPPVKQKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B211</accession>
    <name>A0AAF1B211_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0522596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042045953" evidence="2">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="72" mass="8114" checksum="F00CC3DB930A0D48" modified="2024-05-29" version="1">MGGFKLLLLFLFCLICVRSGGQLFSSAAESVDHQQEEQVFGPHRPVNDTTKDFDVDKRKVPTGSNPLHNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B247</accession>
    <name>A0AAF1B247_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B247"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF470">
        <property type="entry name" value="CYSTEINE-RICH RLK (RECEPTOR-LIKE PROTEIN KINASE) 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="285" mass="32285" checksum="BD50F48BBE3F1C66" modified="2024-05-29" version="1">MITVRLFLSVAAVRNWSVSQLDVTNVFLHGDLPETVYMRLPPGYAYLSDQCPSNSHEYVCKLVKSIYGLKQAPRRWFVKFSTALKEYNFQQSHSDNSLFTLHKNDQFVAVLVYVDDILMSGSSDKLIAEVKAYLLTKFKIKDLGPLKYFLGIEVARSAKCFYLNQRKYVLDLLRDTGMTVAKPSTIPIEQNHSLLDNQSALLKCSEASTYRQLVGRLIYLTITRPDLSYTVHVLSQFMNCPRVDHLYAVFRVLRYLKQSPGQGLLISATSPLTLTACCDSDWGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2F7</accession>
    <name>A0AAF1B2F7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-ascorbate oxidase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0623019</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2F7"/>
    <dbReference type="KEGG" id="dcr:108224943"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13846">
        <property type="entry name" value="CuRO_1_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13894">
        <property type="entry name" value="CuRO_3_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000029">
        <property type="entry name" value="L-ascorbate oxidase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000012">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000016">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034273">
        <property type="entry name" value="CuRO_1_AAO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034275">
        <property type="entry name" value="CuRO_3_AO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF387">
        <property type="entry name" value="OS04G0561900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042183024" description="L-ascorbate oxidase homolog" evidence="3">
        <location>
            <begin position="21"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="31"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="157"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="374"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="59825" checksum="8DB02E74BED9D607" modified="2024-05-29" version="1">MQLSLAAVLVSALVVASVQAEDPYRYFNWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVFNSLNQPFLISWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIRILSRPRIPVPFPDPSGDYTVLIGDWYKSNHMKLKAILDNGRKLPFPDGVLINGRGPNGVSFNVEQGKTYRLRISNVGLENSLNFRIQGHKMKLVEVEGTHTLQSTYSSLDIHVGQSYSVLVTADQPAKDYYIVVSSRFTSKILTTTGILKYSNSAAPVSGPLPGGPTTQIDWSLNQARSIRSNLTASGPRPNPQGSYHYGMIKTSRTIRLSSSAGQVHGKQRYGINSVSFVLADTPLKLADYFKIGGVFRIGSISPNPTGGGLYLDTSVMGADFRAFVEIVFENPENIVQSYHLDGYSFFVVGMDGGQWSSGSRNNYNLRDAIARCTVQVYPRSWTAIYVPLDNVGMWNLRSEFWARQYLGQQFYVRVYTNSNSIRDEYPIPKNARLCGRAAGRHTRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5G8</accession>
    <name>A0AAF1B5G8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0623309</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20401">
        <property type="entry name" value="Tudor_AtPTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056618">
        <property type="entry name" value="Chromo_PTM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047365">
        <property type="entry name" value="Tudor_AtPTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508:SF1">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24294">
        <property type="entry name" value="Chromo_PTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21743">
        <property type="entry name" value="PTM_DIR17_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DDT" evidence="9">
        <location>
            <begin position="188"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="413"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="936"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1411"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH03906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1683" mass="187389" checksum="9F91168B85C801A9" modified="2024-05-29" version="1">MEAGVVQVERRGRGRKRKIEEIENVCVDGLKRETRSKALVGSYVNKEFEGSFYLGKVVSYDRGLYRVEYEDGDSEDFESGELRPFLIENDCHDSILVLRKKELDEIILNKYEKERVDKMRGADVIGGVEDSPLGEVMDHGANGVIADVDCSSDSGEDGLDGALCVDAEACIVLPPEFPPSTGNIGVPEESVAELLSVYSFLRSFSVSLCLCPFGLEDFVGSLNCSVQNTLLDAIHVALMRVLRRHFEALSSEGSEFASKCLSSMDWSLIDSLTWPVFTAEYMMMMGYTEGPKWKGFYVDALQMEYYSLTVSRKLVILQILCDDVLDSAELRAQVDMREESEVEVDRDGDTIATHVGPKRVHPRYSKTSACKDQEATEFMKETGDAKLSINSSSLGYNNNGLDAGTDGDQDANSDECRLCGMDGTLLCCDGCPSSYHSRCIGVSKLCIPKGDWYCPECAINKISPKITGRTSLIGAEFFGVDPFEQVFLGTCDHLLVLKVSMNTGSIVRYYHKEDLSRVVLALCSEAQHLDMYSGICERIIKYWQIPANIIPIAEKVETGLSSIKIEDGSCPTPVSSLLNRNGHGICEPSVSEDIASCIAESSSENIAGPSAGNLTIENDSSKASLNMHIETGHPLTHPCNSGSIEQDRPFQAEKLSEQIRGTATISSDSVSHQDDLSETTQLKLVGRSSQRDHAMCTSGNSNSCNRGQSNCMISIKSCSNIGDGCFYLGSSFKPQAYMNHYIHGDFAASAAANLVKLSSEENYMLVSKTSNNYKKAMSANIALQIKAFSSAVNRFFWPNSEKKLMEVPRERCSWCLSCKAMCQSKRGCLLNAAASNAIKGTMKILSGICPVKLKHGQGSLHSIATYVLLMEESLRGLTVGPFKTLDYRKKWCQQAEQASTCSAIKSLLLELEENMNQVAFSGDWFELVDGWSVESSATNGTSAAEPRQKRGPGGRFSRKLSAIPEIKADDDLDMSNNFVWWRGGILSKFMFHKGILPQILVKRSARQGGSRKISGIYYAEGVETPKRSRRFIWRAAVEMSRNVSQLSTQVRYLDIHVRWSDLLRPDQSSLEGKGAETEAYVFRNASICDKQILGSKVRYAVSFGNQKHLPSRIMKNVIGVEKHEDETEKYWFLETHIPLYLVKEYEEKMDNVHLRLAEKPMNVLSKMQKQQLKVSRKDIFFYLTQRRDNLESCRCASCQLDVFLGNAVTCSKCEGYCHLQCTSSPKVHMSVEVEFVMTCKRCYQTEATLPNEKYPASHLLLQRQKPLNAATAKKSEKQNGCHRPLASNGALQHSVRKKPASNLKPRNKSKDCFWGLVKKKNGEDGTDFRLKNILLKGNLKLSEVECDLCKKPYDSNLIYICCEICTKWYHADAVELEESKILEVTGFKCCKCRRMRSPRCPYADQEETDALESKKSNKRASKQASQVAGSYHETIPEQLTQGDSATHMLPIKKGLVCIKGDNPLTFSLSRMNNGTQQTSEVALEQNPTFSGSVPQKLPVRRHLKQDDDVVGYSTNNTSVDSPKPIPGSTFLPVDESAPCLEWDVSTNIEDDFMFDVEDLNYEDGEFEPQTYFSFNELLAFDDGVQLDGIDPSGNIIVDVDDSSVIPEDVNLEQYGIVTEQQEHLDSFESSFQVVPCQTCFFTDPIPDSCCQICGSCMHSHCAQWVVDTSNNGAWRCGSCRNWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5R6</accession>
    <name>A0AAF1B5R6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0626619</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5R6"/>
    <dbReference type="KEGG" id="dcr:108227924"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="546" mass="58951" checksum="CC7C9213A6A3C4D5" modified="2024-05-29" version="1">MGLSSQSYGIQSMLKEGHKHLSGLDEAVLKNIDACKQLSTITRTSLGPNGMNKMVINHLDKLFVTNDAATIVNELEVQHPAAKILVLAGKAQQEEIGDGANLTISFAGELLQNAEELIRMGLHPSEIIIGYTKAINKTIEILNELIEDGSDTMDVRNKDQVTLRMKSAVASKQFGQEDILCSLIADACIQVCPKNPANFNVDNVRVAKLVGGGLHNCAIVRGMVLRNDAVGSIKRMEKAKVAVFVSGVDTSATETKGTVLIHSAEQLENYAKTEEAKVEELIKSVAESGAKVIVSGAAVGEMALHFCERYKLMVLKINSKFELRRFCRTTGAVAMLKLGQPNPDDLGYADSVSVEEIGGARVTVVRNEEGGNSVSTVLLRGSTDSILDDLERAVDDGVNTYKAMCRDSRIVPGAAATEIELARRLKEFSFKETGLDQYAIAKFAESFEMVPKTLAENAGLNAMEIISTLYAEHASGNTKVGIDLEEGACKDISTMSIWDLHVTKFFALKYAADAVCTVLRVDQIIMAKPAGGPRRDAQPAGPMDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B664</accession>
    <name>A0AAF1B664_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0624082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B664"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="46" mass="5492" checksum="6493512770CFF6D5" modified="2024-05-29" version="1">MDVHLPNMDGLQLLKCINKDYDIPVICKFLKYVSLSYKSWLYINVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAP4</accession>
    <name>A0AAF1BAP4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alginate lyase 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0831959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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        <text evidence="1">Belongs to the MNN1/MNT family.</text>
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    <dbReference type="KEGG" id="dcr:108197224"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042058154" description="Glucuronosyltransferase PGSIP8" evidence="6">
        <location>
            <begin position="22"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH12201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="53776" checksum="2A9BF4E972E4B632" modified="2024-05-29" version="1">MIMRVVSLVVVVLVVVVKVTAEGSGGVAENGSGERKRKNAYATMMYMGTPRDYEFYVAMRVMLRSLYDLKVDADLVVIASLDVPLPWIRALEQEDGAKVVRVENIKNPYRSYRKTRRFKLSFNKLHAWSLVQYERVVMLDSDNIFIRKPDELFQCGQFCAVFINPCIFHTGLFVLEPSTEVFKDMVNKLESGSNSQDGADQGFLVSYFPDLLDQPMFHPPQDGSKLDGTYRLPLGYQMDASFYYLKLHWSIPCGANSVITFPGAPWLKPWYWWSWPVLPLGLQWHQQRRQTIGYGADMPYAIAIQSIFYLGIITLTRMIRPGSISKHFYLRTEKRIILFQTVLKLIATLSILLAYIIPYFLIPHTIHPIMGWSLYLLGVFALSCVTVNALSLPLLPVLTPWMAIIGALSVMACTLYPDGVIRASAVFGYAFCSSPVVWLALIKVMNSVQVSFEREALLPKLSELTPPTGFNKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDV9</accession>
    <name>A0AAF1BDV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0935331</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDV9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF112">
        <property type="entry name" value="RECEPTOR LIKE PROTEIN 30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042000041" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="95"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH15785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="79122" checksum="5BA3A0235810F77D" modified="2024-05-29" version="1">MNLLVLACALAFLSTSYLGCQGTDLSEDSRNKTCIAGEKQVLLDLKKILIDQDKWLESWVGDYCCSWQGIGCDKDGHVTELDVGDCSSKGDSIIPFLVRNLTFLTTLTLSQNNFQGLIPKSIGDLKSLVTLELSFNNLSGPLPESFCRLSKLEKLDVGDNQLSGTIPKCIGQLSTLKWLDLFQNSWEGVLSEQQLVNLTSLTFLSISSKSKLVYHVSSEWIPPFQLQGFTIENMRVGPEFPPWLLTQEELLLLKMSNSSLVGTIPADWFGNLLSHAVHVDLSYNDISMARPSFISAPNNLSTLRLSNNRLSDEFPAYICNFSSLTALLLSDNNFTGELPRCLGNLTQLEELDVMDNHLSGHVPDVFGSLERLSYLNFYNNSFEGKLPLSFQNLTQLLIFDVGNNNFRDALPTWTSQQIPYLTYIGLRANHFYGTIPIQLCNFSSIQIINLARNHITGNIPQCFNNLTAMTTNETGIRYYFGDTLVGGKVLIDDVKGYELKYTSTLDFFYSINLSDNNISGEIPEELMDLRNLLNLNLAGNNLVGKIPARIGKIEQLEFLDLSRNKLSGPIPQSLSELKFLVRLNLSFNDLSGRIPTGNQLQTLDNLSSIYAGNNQLCGRPILKLCSGDAKPHEGHDYEKDNSDNDSDSDEHVWFYAGIGPGLLVGFLGFCSSLHYMKSWRHSYFNFVEMVLDKIAVFIALSWKKFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEP8</accession>
    <name>A0AAF1BEP8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DEAD-box ATP-dependent RNA helicase 50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0832655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BEP8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="320"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="351"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="568"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="167"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="320"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH13146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="726" mass="81767" checksum="DF5D0B2AC01985CD" modified="2024-05-29" version="1">MLAKLPPAISFEFRRTEALRRPSLSFTTTCKNHNNDNATSYTPKWRRKCSDSGGGGSRVSIVARAAYTRRKLETAGAYELIDDETGEKVIVWGDEKDDDDVDDSGVSKDVLKWKPEKKISVSASRRSGSDSESRGNGWVIYVCVSGNKSLAGNFGRLKVQKVRKLARATSSSQQTVRGREYVDKASKGTFSDTSADSPDENEKLAVRRNFDALRDKLNVTRAGRSQDKQDTTQRKAMTREDMEDYEDVDEDEVDEVPKHQTRSPKYQRTDSRASDTRTSASSFRGWSRGVPMEDEYEYRPTVYPKKGKKGNADSDFFSRKSFKDMGCTEYMMEALKSRLFNRPSHIQAMAFAPVFEKKSCIIADQSGSGKTLAYLLPVIQRIRQEELEGIGKSLPQNPRVVILVPTAELASQVLTNCRLMSKFGVPFRSMVATGGFKQKTQLESLRQELDVLIVTPGRLTYLVKEGFLQLTNLTCTVLDEVDILYSHEDFEPALQILMNSSPYTTQYLFVTATLPVNIYNKLVEVFPDCEVIMGPGMHRTSPGLEEILVDCSGDDGAEKTPETAFLNKKTALLRLVAERQVAKTIIFCNKIETCRKVENALQRFDRNEYRVRALPFHAALAQETRLANMEEFRSSQRKDSLFLVCTDRASRGIDFTGVNHVILFDFPRDPSEYVRRVGRTARGAGGVGKAFVFVVGKQVSLARRIIERNLKGHPLHDVPSSYEQMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BFI0</accession>
    <name>A0AAF1BFI0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance protein RPS4B/Roq1-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BFI0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058546">
        <property type="entry name" value="RPS4B/Roq1-like_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF517">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23286">
        <property type="entry name" value="LRR_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein RPS4B/Roq1-like leucine-rich repeats" evidence="2">
        <location>
            <begin position="132"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH14962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="403" mass="45681" checksum="0ADB432871892A7D" modified="2024-05-29" version="1">MGREITCQQSLKEPGQRSRLWYYKDSLEILSDQTGTGVLEGVALDMNMTNACQTELQTVAFSMMRKLRLLKLNNVQLSGGYTDFPKKLKWLTWHNFSLRALPNGFPLSSLVAIDMQSSKLQRLVQGNMLLGSLKFLNLSHSHDLVKSPNFAEFNALEQLILEDCVSLIEIDESIGMAEGLVLLDIKDCKLLKRLPKNIYKLKCLETLIISGCSNIRMLDAEMKNMESLKDFHADGLDFGNSSYRNQKSESWLDFFQGLDYCLAPSLQYLIFSGNPIRFLPDCFKGLERLKELHVREYSQLQALEDLPNIQNFLVALDCSVLEKITFDAPGTVLKGFAIPNGCGNLLEMQSRFKIVPIGDIDSQFINNCGIYDVEVKKRMQVKLHNTYTETATRCPIQVISLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WTC3</accession>
    <name>A0A161WTC3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_010659</name>
        <name evidence="6" type="ORF">DCAR_0312114</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WTC3"/>
    <dbReference type="STRING" id="79200.A0A161WTC3"/>
    <dbReference type="Gramene" id="KZN01905">
        <property type="protein sequence ID" value="KZN01905"/>
        <property type="gene ID" value="DCAR_010659"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210786"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="189057at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030899">
        <property type="term" value="F:calcium-dependent ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02036">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000068">
        <property type="entry name" value="Site-determining protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010223">
        <property type="entry name" value="MinD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01968">
        <property type="entry name" value="minD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF6">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="4">
        <location>
            <begin position="63"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN01905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="328" mass="35771" checksum="531FEB580318665A" modified="2016-07-06" version="1">MISLLTHSNPLSYLPHSSPTPSISPFFTHTKTLKPSKLKSLKINSILQYNRKPELAGDTPRVVVITSGKGGVGKTTTTANIGLSLARLGFSVVAIDADVGLRNLDLLLGLESRVNYTVVEVLNGDCRLDQALVRDKRFSNFELLCISKPRSKLPMGFGGKALVWLVDALKDREEGCPDFILIDCPAGIDAGFITAITPANEAVLVTTPDITALRDADRVIGLLECDGIRDIKMMVNRVRTDMIKGEDMMSVLDVQEMLGLALLGVIPEDSEVIRSTNRGFPLVLNKPPTLAGLAFEQAAWRLVEQDSMKAVMVEEEPKKRGFFSFFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WUK4</accession>
    <name>A0A161WUK4_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0626050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WUK4"/>
    <dbReference type="Gramene" id="KZM92277">
        <property type="protein sequence ID" value="KZM92277"/>
        <property type="gene ID" value="DCAR_020358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH06622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="385" mass="43926" checksum="2E26CF8414CB0186" modified="2016-07-06" version="1">MGGADALTDDILTEILKRLPKKALFRFKCVNKSWNRLIQTPYFGALQSDHNKTNLRNIYMLVFLHWGNLALYGIDPVHGLGYNSDSTDLHDLHPLLVFPTGRRMSHSVIGFRTETRFEVFSPVDEAWHVLPTPHDDNAVPGDFDDYEFTYLFLEKSEQVVYFAVVGYILSFNLKTHKWNPASPHRLTMLPPVSIVNGIAFGFDNSYASPHRPTSFPPLTIVNGISFGFNSYVNICASKPLSKGEEVMQPYLAADKDFLEVLGSPSRFTYMTKRKMFYEDYIIGLEDSDNKEVMCFLTHGSKPAEDPISCPDYKTSYVALSIFRICNDLYTEKRPASEHYSTYADEEGEDGAVVRRYFNAKFLYTKHFIISNSRLHAMGMIVTCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X762</accession>
    <name>A0A161X762_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0208537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X762"/>
    <dbReference type="Gramene" id="KZN06725">
        <property type="protein sequence ID" value="KZN06725"/>
        <property type="gene ID" value="DCAR_007562"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205614"/>
    <dbReference type="OMA" id="NANCHIH"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF88">
        <property type="entry name" value="REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="500" mass="56092" checksum="2420D6807020F023" modified="2016-07-06" version="1">MFDGLLKSKFQTKCKSLVKMTKTRLEMIKKKRSAMQKYLKNDIADLLQNGLDSNAYSRAEGFLKEMKLSSCYEYVELACSCISTHLAAMSKNRECPEDCREAVASLMFAAARFADLPELRELRSLFTHRYGKSVDGYANKEFVAKLKLLPHSKDKKLQLLQDIAHESGIEWNSKALEQKLYKPPAVEENVSKNTSAVDRNKQNDQRVTSGAKEQDICSDKKEVNVSKNTSAVDRNKQNDQRVTSGVKEQDICSDKKEEVTYDDKKVTKSRRTPRNGFQAKKMDQDVRPSDPVRSSSKEKLDGGKTASSYSSIPPPYTRQPVSKTDPILDVPSGGSDVRGEESRKLDAQPDKESKPKPKSVRRKPAKLPNPPPGQDYIGSLKGDETATNSDGTTEEDANQIGHDLSDEEERKMDKLLMLYSNKNVAPEASKEPKSRHREFSLESRAASLPLETTSPVETKRGHARATSYETDMNANCHIHPKLPDYDDFVARLAAFRGEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XII4</accession>
    <name>A0A161XII4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0208775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XII4"/>
    <dbReference type="Gramene" id="KZN06939">
        <property type="protein sequence ID" value="KZN06939"/>
        <property type="gene ID" value="DCAR_007776"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206382"/>
    <dbReference type="OMA" id="GARSANW"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011949">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47105">
        <property type="entry name" value="OS02G0173600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47105:SF1">
        <property type="entry name" value="OS06G0665100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG89537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="28728" checksum="608AFBFEE8EB30D5" modified="2016-07-06" version="1">MSIMTKLRCITLDVTGTLIAYKGDLGDYYCMAAKSVGLPCPDYKRVHEGFKIAYTKMAKEHPCFGFAEKIPNIVWWKTCVRDSFVKAGYDYDEETFEKVFKRIYSTFGSAAPYTMFSDSKPFLRWARDEGLLVGLVSNAEYRYRDVILPAMGLNQGSEYDFGVFSGLEGFEKPDPEIYKIALKRAGNIAPEEVLHIGDSMRKDYVPAKSIGMHALLLDRFKTPDAEEWRKSGATVLPDLVAAQEWLTSDQSTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y5W7</accession>
    <name>A0A161Y5W7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Beta-galactosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0728456</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="4 11"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y5W7"/>
    <dbReference type="Gramene" id="KZM87669">
        <property type="protein sequence ID" value="KZM87669"/>
        <property type="gene ID" value="DCAR_024770"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196472"/>
    <dbReference type="OMA" id="TYNTAKI"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22842">
        <property type="entry name" value="Gal_Rha_Lectin_BGal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000142">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000098">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH09005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="823" mass="92033" checksum="779CF6D13D3A2B60" modified="2016-07-06" version="1">MAAKALTLLLLCLVTVCNCMEVTYDSRSFMIDGERKLLISGSIHYPRSTPEMWPDLIKKAKAGGLNMIETYVFWNAHEPEQRKYDFTGNLDLMRFLKTVRDEGMYIMLRIGPYVCAEWTFGGFPVWLHNLDGVKFRTANDVFMNEMQNFTTLIVDMAKKEKLFARQGGPILITQIENEFGNVEHPYGEAGKVYMNWAANMAVSLDTGSPWIMCQQKDAPPIVINTCNGWYCDDFTPNNASSPKIWTENWAGWFMSWGSVRPKRAAEDVAFAVARFYQTGGSLQNYYMYHGGTNFGRTAGGPYITTSYDYDAPLDEYGNLNEPKYSHTKQLHNLLMSMEKPLTEGKVTHIDFGNGAEGTVFVDGQTTSCFFSNKNTSSDALIKYQDMNITVPAWSVSILPDCQTEAYNTAKVNTQTSVMVKRENGAENQPSALKWSWRPENLDVAVIQGKGSIHASEILDQKVLNDMSDYLWYMTSVDIDEKDPVWGTNMSIRINGTGHVLHAYVNGEYVGSKWAKYDVHNYDFETPVKFKPGRNQISLLSALVGLKNYGAFFDDYRAGVCGFVDIVGRNGDEIVTKDLAGHQWVYKNSLHGLDNQLYSQTSKFSAKWQLGYLPVNRSMTWYKTTFKAPLGKDPVVLDLLGLGKGYAWVNGNNLGRYWPSYLAPEDGCDDECDYRAKYDASQCNYGCGEPTQRWYHVPRDFLSATGVNELVLFEEFGGNPSQVNFKTVVVGSACGSAYEHKTMELSCQGRSITQIKFASFGNPMGTCGSFKKGTCEGSKDALSILQKACIGKETCSLVANENIFGPTSCADSPKKLLVEAVCAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YGS7</accession>
    <name>A0A161YGS7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0626052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YGS7"/>
    <dbReference type="Gramene" id="KZM92279">
        <property type="protein sequence ID" value="KZM92279"/>
        <property type="gene ID" value="DCAR_020356"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225340"/>
    <dbReference type="OMA" id="EECELHH"/>
    <dbReference type="OrthoDB" id="1026046at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF29">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="125" mass="14854" checksum="2D43EC4AF2B2AE05" modified="2016-07-06" version="1">MGSGDFKNLLNFHLHIHHHHHHHNHHNNHKKEMKEIPKGCLAVMVGQGEEQQKFIIPVIYINHPLFMQLLKEAEEEYGFDHHGPINIPCHVEEFRNVQVMIDQENSHHHGHAHHHHNHHVWCFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZJS5</accession>
    <name>A0A161ZJS5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_024406</name>
        <name evidence="3" type="ORF">DCAR_0728214</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZJS5"/>
    <dbReference type="Gramene" id="KZM87272">
        <property type="protein sequence ID" value="KZM87272"/>
        <property type="gene ID" value="DCAR_024406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM87272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="76" mass="8196" checksum="35A3070251EB5D21" modified="2016-07-06" version="1">MRMHTQSHIIKMSVASCLKLLLIFGVMLLFIAPSLAIRPVTYPALKPGPVVIPRAPPGVPSRYHPPPASPTENTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZS65</accession>
    <name>A0A161ZS65_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_023237</name>
        <name evidence="3" type="ORF">DCAR_0622768</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM89400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZS65"/>
    <dbReference type="STRING" id="79200.A0A161ZS65"/>
    <dbReference type="Gramene" id="KZM89400">
        <property type="protein sequence ID" value="KZM89400"/>
        <property type="gene ID" value="DCAR_023237"/>
    </dbReference>
    <dbReference type="OMA" id="PKHGEED"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37202">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37202:SF1">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM89400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="213" mass="23532" checksum="3BDE31257DB4DA16" modified="2016-07-06" version="1">MSGREVREYTNLSDPKDKKSGKGRIDDEDTTFQRMVAKMQEVAGERGGYLHGRGALDSDDLLYLKEQMEAEEDAERLLRRTEKRAFAAFKISFLRTIISLTALNKAASIADSSPATIPLPLRVEPKPKSGIRQQDLLKKVVEVKPKRPRVATPHDNKNVTVSAITGHSANSKPEANQGEKVAPSVDGDKTKNNMDNPVKSLLVAYVNSDSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ADI9</accession>
    <name>A0A162ADI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_013398</name>
        <name evidence="6" type="ORF">DCAR_0417642</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ADI9"/>
    <dbReference type="STRING" id="79200.A0A162ADI9"/>
    <dbReference type="Gramene" id="KZM99240">
        <property type="protein sequence ID" value="KZM99240"/>
        <property type="gene ID" value="DCAR_013398"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217782"/>
    <dbReference type="OMA" id="CLNVKCG"/>
    <dbReference type="OrthoDB" id="1487578at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000682">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g16610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000450">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF503">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="32"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="570"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM99240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG98301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="641" mass="71403" checksum="E8E5E5A55746013D" modified="2016-07-06" version="1">MISRSANSLCYSSTTILRFNTRYICSTSNTSSCSSWNTQLRELAKQGHYQKALYLYPQLLSSGASPNAFTFPFALKSCAALCLPVPGQQLHAHVIRTGCHSEPFVQTALMSMYCKCFLVENARKLFDESTYARRLTVCYNALIAGYTHNSEFCDAVLLFRRMRLLGVAVNSVTMLGLVPGCTVPLHLGFGMSLHCLNVKCGLDGDMSVGNCLLTMYVRCGSVWSGRKFFDEMPEKGLISWNAMISGYAQNGHANLVLDLYREMELKGVSPDPVTLVAVLSSCANLGASEVGMEVEQKIKRRGFGSNQFLQNALVNMYARCGNLVKAQAIFDDMPEKNLVSWTAIIGGYGIHGHGDDAVKLFDQMIDAGIRPDKTVFVSVLSACSHAGLTKRGLEYFDSMEKKYRLQPGSEHYSCVVDLLGRAGRLKEAQELIESMSGKPDGAVWGALLGACKIHRNVELAELAFERVIELEPTNIGYYVLLSNLYSETKYTEGILKVRVMMRERKLRKDPGFSSVEYGGRNHLFMAGDRSHPQTEEIYAMLNRLEDLAKETSERRKIDDDGGNSELVSCARVHSERLAIAFALMNTALGSRILVTKNLRVCTDCHIFIKLVSKIVDRQFVIRDATRFHHFQNGVCSCNDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AL40</accession>
    <name>A0A162AL40_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0103323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AL40"/>
    <dbReference type="Gramene" id="KZN10486">
        <property type="protein sequence ID" value="KZN10486"/>
        <property type="gene ID" value="DCAR_003142"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214318"/>
    <dbReference type="OMA" id="DAYHNCF"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="94" mass="10738" checksum="069B47A0643E7CC7" modified="2016-07-06" version="1">MGFLRSEKKAPTPASTSPCANLRAAYDKCFNRWYSEKFLKGQWDKEECVSEWNKYRDCLSQHLDDKHLARFLEAEGIGNMIHQVDASSPKGAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AUM0</accession>
    <name>A0A162AUM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U6 snRNA-associated Sm-like protein LSm8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="10" type="ORF">DCAR_0207884</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AUM0"/>
    <dbReference type="Gramene" id="KZN06143">
        <property type="protein sequence ID" value="KZN06143"/>
        <property type="gene ID" value="DCAR_006980"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206905"/>
    <dbReference type="OMA" id="TLESYMN"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000035">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG88649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="98" mass="10677" checksum="B6268BC7D5CBE90C" modified="2016-07-06" version="1">MSAGAGLENLVDQIISVITNDGRNILGILKGFDQATNIILDESHERVYSTKEGVQQLVLGLYIIRGDNISIVGEVDEELDASLDLSKLRAHPLKPVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162BA73</accession>
    <name>A0A162BA73_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_004539</name>
        <name evidence="16" type="ORF">DCAR_0104914</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162BA73"/>
    <dbReference type="STRING" id="79200.A0A162BA73"/>
    <dbReference type="Gramene" id="KZN11883">
        <property type="protein sequence ID" value="KZN11883"/>
        <property type="gene ID" value="DCAR_004539"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205261"/>
    <dbReference type="OMA" id="WQQEGFN"/>
    <dbReference type="OrthoDB" id="10257567at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="14">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="13">
        <location>
            <begin position="432"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="423"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="504"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZN11883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG85722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="682" mass="78989" checksum="C5BA809A31AA0B70" modified="2016-07-06" version="1">MESSGGSERDKLNSSSTPVPSVANFWKEFDLEKERSVLDEQGLKIAENQENSQKNRRKLAESTRGFKKASAEEKLSMFNTLLKGYQEEVDNLTKRAKFGENAFLNIYQKLYEAPDPYPVLSSIAEKDVKLMEIESENRKMKVELEEFRTEATHLKNQQATIRRLEERIRQLEQQMEEKVKEVVEIKQRSLAEENQKSLEVLKEREQLLQDQLRQAQDSVSNMQKLHELAQSQLFEYRAQSEEDRAAKQSEVNLLMDEVERAQTRLLSLEREKGVLRSQLQTVNEEHGNDKSDDMDPNSVLENSLNAKEKVISELNMELHNMETTLSEEREQYMKEIKKLSALLNEKDAALVETKKELHARPTEKLVDDLRKKVKILQAVGYNSIEAEDWEVATSGEELSQLESLLLDKNRKMEHEVTHLKVKLSETTSLLETAEGKITELTAKLSEQQMLIQKLEDDISKGYNAKDRKGALFDDWELPESGTTGQSDNTDQKHVSSEQDQNSMLKVICNQRDRFRTRLRETEEEIRQLKEKIGSLMAELEKCKADNVKLYGKIRYVQDYNLEKVVSRGSKKHVDDLESGFSSDVESKYKKIYEDDINPFAAFSKKERDQRYKELGFRDKITLRSGRFLLGNKYARTFAFFYTIGLHALVFTCLYKMSAYSNLSHGPEEFLATEKNIDLPHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UBT5</accession>
    <name>A0A164UBT5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_025759</name>
        <name evidence="4" type="ORF">DCAR_0729603</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UBT5"/>
    <dbReference type="STRING" id="79200.A0A164UBT5"/>
    <dbReference type="Gramene" id="KZM88684">
        <property type="protein sequence ID" value="KZM88684"/>
        <property type="gene ID" value="DCAR_025759"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193161"/>
    <dbReference type="OMA" id="DDTVYAC"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000360">
        <property type="entry name" value="FAD-dependent oxidoreductase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000033">
        <property type="entry name" value="Putative oxidoreductase C1F5.03c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="48"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM88684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH10141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="416" mass="43391" checksum="E524F0B9BF4880DA" modified="2016-07-06" version="1">MSLTSSAHFSVPPNPLFSVKTTTIAASRIKASSTMDNNPQSTNSPSKKVVVCGGGVIGVCAAYYLSKKGAAVTLVEKSSIACAASGKAGGFLALDWCDSGPLSSLARASFNLHRSLAQELDGYNTYGYRPLTTLSLSVTESKSGIGPGPGAPQWVDGPAKQVRTIGTPETTAQVHPQLFTRTLLSRAVAEHGVEVVIGKLESVVAEGGCVTAVELEGGRVVEADAVVLALGPWTSKLLMLNELFRVYGLKAHSIVLEPKEADAISPHALFLSYYAAGGGKPMDPEVYPRPTGEVYICGMSAEVEVPDDPEEVFPNPESIEVLKKVAKNVSSHLAEGEATVKAEQACFLPCTDDSAPVIGEVPGVKGCFVATGHNCWGILNGPATGAAVAELVIDGHATIVDLGKFSPARFIGRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UP50</accession>
    <name>A0A164UP50_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0730117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UP50"/>
    <dbReference type="Gramene" id="KZM89157">
        <property type="protein sequence ID" value="KZM89157"/>
        <property type="gene ID" value="DCAR_026232"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH10648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="23612" checksum="E8526F725128FED0" modified="2016-07-06" version="1">MEKNRALKEKFKELEQHEYWDHSNNSSSVFDLMSRSPRELMSSLQDEEEELLSSNVSSVCSDCLEDEESEDEEVRSVVVSSGSVYDDKLSNEDVDNGINRIYEEGKKVEINKVSLCNEDVDDGINKIYDGGKKVEISKRSSADVKNAEKIRKVGWEFSVLISVLVLIGLVIAVMFSVRSTWYVDQGLDQASKGPYHYPWAMILQAQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UWL7</accession>
    <name>A0A164UWL7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0622846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UWL7"/>
    <dbReference type="Gramene" id="KZM89475">
        <property type="protein sequence ID" value="KZM89475"/>
        <property type="gene ID" value="DCAR_023162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="149" mass="17349" checksum="B13C8BC4D96087B5" modified="2016-07-06" version="1">MFSGNHFYVICFNLKKITVEIIDNRSGDRVDTMYDGIPETMQENFGLYIAQQSPKKSMLLSNAPVQRLQMKWRTSNKNVDSGVFAMHHMETYMGYGLRNWECKFAAEVGIEQKRQLERARQIYATKIVYSGINFLKGQMTTEIKFVNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W3R8</accession>
    <name>A0A164W3R8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inositol polyphosphate multikinase</fullName>
            <ecNumber evidence="8">2.7.1.140</ecNumber>
            <ecNumber evidence="8">2.7.1.151</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_021389</name>
        <name evidence="10" type="ORF">DCAR_0624899</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Inositol phosphate kinase with a broad substrate specificity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>1D-myo-inositol 1,3,4,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>1D-myo-inositol 1,4,5-trisphosphate + 2 ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.151"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.140" evidence="8"/>
    <dbReference type="EC" id="2.7.1.151" evidence="8"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W3R8"/>
    <dbReference type="STRING" id="79200.A0A164W3R8"/>
    <dbReference type="Gramene" id="KZM91246">
        <property type="protein sequence ID" value="KZM91246"/>
        <property type="gene ID" value="DCAR_021389"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227987"/>
    <dbReference type="OMA" id="DCAFAAT"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051765">
        <property type="term" value="F:inositol tetrakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF51">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM91246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH05482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="31239" checksum="D3B7E81FAA015F93" modified="2016-07-06" version="1">MFKAPSHQVAGHQARDGKLGPLVDELGRFYKPLQSGERGSQEVAFYEKFCSNTTIPDHIRNFFPIFYGTQLLEASDGSGKHPHLILQDLVSGRVNPSIMDIKIGSRTWPPESPEDYIQKCLQKDRGSTSLPLGFRISGLQVNGGEESASWKPEKKYIKSLTADDVRLVLRKFVSSNVSAESSVQPDCSLASIVYGGSTGILAQLLQLKAWFEEQTVFHFYSCSILFMYEKGSMLEGKKSNAEIKLIDFAHVLDGCGVIDHNFLGGLCSLIKFIREIIDTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W9A6</accession>
    <name>A0A164W9A6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W9A6"/>
    <dbReference type="Gramene" id="KZM91461">
        <property type="protein sequence ID" value="KZM91461"/>
        <property type="gene ID" value="DCAR_021174"/>
    </dbReference>
    <dbReference type="OMA" id="AWATPIT"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36405">
        <property type="entry name" value="BNAA10G09140D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36405:SF1">
        <property type="entry name" value="OS07G0520600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="165" mass="18847" checksum="9246E3C7BC248912" modified="2016-07-06" version="1">MKVNWNFRSHLAHEQNTIKRQPGSIRVGATGKVSTLMTHELDEVKGSSSHHVSRKRNTQNFPVTVYCGESRRQACYQSGNSTTSTGQRAETRQKQQHNMRHTGRDIPVFASEDASVHMKPVRDEKRKPQYIEVVDLKCGTSLKKAWSRHTTKNRSLKFSRLSVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WIG1</accession>
    <name>A0A164WIG1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TPM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_020819</name>
        <name evidence="3" type="ORF">DCAR_0625523</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WIG1"/>
    <dbReference type="STRING" id="79200.A0A164WIG1"/>
    <dbReference type="Gramene" id="KZM91816">
        <property type="protein sequence ID" value="KZM91816"/>
        <property type="gene ID" value="DCAR_020819"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193028"/>
    <dbReference type="OMA" id="NNCAEVK"/>
    <dbReference type="OrthoDB" id="417797at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007621">
        <property type="entry name" value="TPM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35514">
        <property type="entry name" value="THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35514:SF1">
        <property type="entry name" value="THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04536">
        <property type="entry name" value="TPM_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPM" evidence="1">
        <location>
            <begin position="97"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM91816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="228" mass="24839" checksum="BA333F8A2B24C77F" modified="2016-07-06" version="1">MVGFLLQFPCSVPAFRSSSTNPLLAATVVSAHSDHSLTTQQNSSHFTRWAAAFPSKSLSFALSATLAISLSLSGIGYAEAKVGVNKPELLPKNFTPVIDVAGFLSEGQENRIAQEITAIEKDTGFKLRVLAQNYPDTPGLAIKDYWQVDDRTIVFVADPTFGNILNFNVGDLVDLDIPRSFWSRLAGKYGNMFYWKEKGEDASIEAAVMAISNCLREPVGPNNCSEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WYC3</accession>
    <name>A0A164WYC3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_020199</name>
        <name evidence="10" type="ORF">DCAR_0626240</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WYC3"/>
    <dbReference type="STRING" id="79200.A0A164WYC3"/>
    <dbReference type="Gramene" id="KZM92436">
        <property type="protein sequence ID" value="KZM92436"/>
        <property type="gene ID" value="DCAR_020199"/>
    </dbReference>
    <dbReference type="OMA" id="KMCGSAK"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000006">
        <property type="entry name" value="Pectinesterase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF87">
        <property type="entry name" value="PECTINESTERASE INHIBITOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854288" description="Pectinesterase inhibitor domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="8">
        <location>
            <begin position="27"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM92436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH06812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="196" mass="21551" checksum="A36FEA489A444CDB" modified="2016-07-06" version="1">MTRSYYLVPFFLLLAFYSEAASSPIPANIGFIKSSCNATAYPDLCISSLSLYATTIQNSPHQMAVMALEVSLNRTKLAQTFMHLLTKFKGLTAKQQEAIADCKDEVEDSLDRVNRSSIEMKSLGQAGGQEFIWHMSNVETWISAALTDDTTCMDGFSDLATEGKIKESVRAQITSVAHYTSNALALINSFAAMQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XEI3</accession>
    <name>A0A164XEI3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0518662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XEI3"/>
    <dbReference type="Gramene" id="KZM93067">
        <property type="protein sequence ID" value="KZM93067"/>
        <property type="gene ID" value="DCAR_016312"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043422128" evidence="2">
        <location>
            <begin position="25"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="12639" checksum="FF7E5147BC0EFE47" modified="2016-07-06" version="1">MTGVIRCLLIIVLCVQLLIVTSFATENKLSMSVKQNLDDHVVAINPRGEEGSKERADHEIKDEVVVSKKSQGKKGSGGGNNVVRDPAGKKKNDAMSKPAEDSSCVVEVVLNWLDFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XSN0</accession>
    <name>A0A164XSN0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">DCAR_016789</name>
        <name evidence="32" type="ORF">DCAR_0519196</name>
    </gene>
    <organism evidence="31">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="31">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="32">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XSN0"/>
    <dbReference type="STRING" id="79200.A0A164XSN0"/>
    <dbReference type="Gramene" id="KZM93544">
        <property type="protein sequence ID" value="KZM93544"/>
        <property type="gene ID" value="DCAR_016789"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223255"/>
    <dbReference type="OMA" id="WNDKMDS"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000463">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF27">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE_ENDORIBONUCLEASE IRE1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="20" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 28" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="21" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854401" description="non-specific serine/threonine protein kinase" evidence="28">
        <location>
            <begin position="20"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="486"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="30">
        <location>
            <begin position="779"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="422"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00065357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="KZM93544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="WOG99840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="913" mass="102103" checksum="0C158A6C4769A480" modified="2016-07-06" version="1">MAILEFITLFLCVIACIYAGYADLSANSGRSEISVAEQNLDGANGFQKFHTTSLLPAKNKHDRALVVAPDGTISLMDVSSKKIIWSFASGPSIYSLYQALPDHEGDNGSFFIDLGQDDWELYMHVNDSTAVKLGFSAEELVRRAPYVSSDTVLLGMKNTTVFLVDAKSGSVIHTFGSAGSPNPVDLIGTKKPIVARKDGLHEPSGPDPLYITRTDYTLKYSSQKTGKVLWYLTFADIEASFQCQRIEKLFSGGSSGDEFCQTKPLVYRIRNLPTLESIMVSDRLGMFPSRGRNPSLPVPGTNHQESRGKTFPALLHSESDNLLDFSQHEQRSLPGGRLDVEGKLVPALPHSDTEGRIFALPEGEHVNAARKSSPEGQPLYSVSFIVQLFVFILSIVAVFFRTRSRKQSMSKLQAQGITAQTATYKKKKTRKPSVMKNNVSTKRSQNEKSGDGSSEVLDFDKVEKKFELAFDCADNDIDGRKVGKILISNKQIAKGSNGTVVLEGNHDGRSVAVKRLVRTHHDVALKEIQNLIASDQHPNIIRWYGVEFDQDFVYLSLERCTCSLYELITTYSDSCQSQSSGKGKDRQTSADATASLLWAVDDIHYLKLWKANGYPSPHLLKLMRDIVNGLAHLHELGIIHRDLKPQNVLIRKERTLCAKVSDMGISKRLSGGMTSLSKQTTGYGSSGWQAPEQLRDERQSRAVDLFSLGCILFFCITGGNHPFGDSLERDLNIVNDKKDLFLIENIPEAMDLVTGLLDPNPTLRPKAVDVLHHPLFWNSEMRLSFLRDASDRVELEDRETQSEILKALEGIGSLALNGKWDEKLENAFLNDIGRYRRYKYDSVRDLLRVIRNKLNHYRELSEEIRGILGVVPGGFDSYFSSRFPKLLIEVYKVIHKYCSEEESFSKYFTSIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YTJ5</accession>
    <name>A0A164YTJ5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_018190</name>
        <name evidence="3" type="ORF">DCAR_0520820</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YTJ5"/>
    <dbReference type="Gramene" id="KZM94948">
        <property type="protein sequence ID" value="KZM94948"/>
        <property type="gene ID" value="DCAR_018190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854728" evidence="1">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM94948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH01436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="81" mass="8990" checksum="69C50DCD0C73A354" modified="2016-07-06" version="1">MSLQRSAFRVLFFIIIVTSQVVSCRVLRAANQTRSFLPSSELQHLSSSSSFEDLTNKNIDITHTYTASKKVVPGGPNPLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A6Q8</accession>
    <name>A0A165A6Q8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A6Q8"/>
    <dbReference type="Gramene" id="KZM96999">
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        <property type="gene ID" value="DCAR_015639"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10040922at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="184" mass="20992" checksum="1B2A54C59B7F6C03" modified="2016-07-06" version="1">MKLPEESGFDLLRDYNWSAKDTEFKIFEKKLMKEELDRCRKKTNAQIMFIAYTTGSFRVGFTMPGGYPQNREPDEEKVLLSKKTAFNAFVTTYGCIVFGIIDIFSFSLADCIFTRRYRPIVYTSTVLNIISVTAMMLTFITGTYNIVSVIAMMLTFITGTYNVVSVIAMMLTFIAGEHITLFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A6V2</accession>
    <name>A0A165A6V2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_015632</name>
        <name evidence="2" type="ORF">DCAR_0415094</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A6V2"/>
    <dbReference type="STRING" id="79200.A0A165A6V2"/>
    <dbReference type="Gramene" id="KZM97006">
        <property type="protein sequence ID" value="KZM97006"/>
        <property type="gene ID" value="DCAR_015632"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217783"/>
    <dbReference type="OMA" id="FQQMFEP"/>
    <dbReference type="OrthoDB" id="1065058at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010237">
        <property type="entry name" value="Pyr-5-nucltdase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01993">
        <property type="entry name" value="Pyr-5-nucltdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725:SF72">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01132">
        <property type="entry name" value="C1.5.3:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM97006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="272" mass="30733" checksum="9E89DED276C98CC3" modified="2016-07-06" version="1">MDYQNQYLSKSGYECLLFDVDDTLYPFSSGLSTQCTKNITEYMIQYLGIDATNASEMCLKLYKDYGTTMSGLRAIGYDFDDDHYHSLVHGRLPYQNLKPDPVLRNLLHSIPIRKVIFSNADEAHVAKVLSILGLEDCFESVICFETLNPTHKSNITDDARNSGEDGNDSALLESPIICKPFKIAFEEAFKIANINPEKTLFFDDSARNLQTAIRMGLHTMVVGSSYRAKGVDYALESIHNIREALPELWETLEKSEEVLYSREAAIETIVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WVC6</accession>
    <name>A0A165WVC6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_014934</name>
        <name evidence="4" type="ORF">DCAR_0415891</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="4">Leaf</tissue>
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        <location>
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        <location>
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</entry>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG92590.1"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Yildiz M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
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        <name evidence="9" type="ORF">DCAR_0103241</name>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the oleosin family.</text>
    </comment>
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        <property type="protein sequence ID" value="KZN10417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="PANTHER" id="PTHR33203">
        <property type="entry name" value="OLEOSIN"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <comment type="catalytic activity">
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        <location>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH14761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="631" mass="70053" checksum="FEAC4CCBD2C3AF45" modified="2016-09-07" version="1">MSANETPQHAEQWNDSEEDDDMEEQNWDDFVADEEDDEEFDSVMLCLFCASTYKSSDELFQHCKAAHCFDFSSLKNDLKLDFYGCFKLINYVRSQVAKNSCWACGCSFGSKEDLHGHLHDIVNFKECTRPWDDDKYLNSFLQEDPLLHNFFEDDEYEGGYNVLADDDELGRIWSGTERMMIDDETDLETMAPECNSPLEDGSKQIVYALATHSISEVPLVKDTVSSSDLSNGGTSFSYPNNKPLQKFSAKISENEIMNVNRSYFGSYSSFGIHREMISDKVRTDAYHRALTDNPSLLKGAVVMDVGCGTGILSLFAAKAGASRVFAVEASDKMAAVASQIAKENGLLCESKAEGSNECSGVINVVHGMIEDVSSEHVKPQSVDVLVSEWMGYCLLYESMLSSVLTARDKWLKPGGAILPDTATMFVAGFAKGATSIPFWEDVYGFSMSCVGRELVEDASKHPIVDVVDPQDIVTNAVVLQTFDLVTLKSEEMNFTATVELEPKFDASKINTGSESRTMWCYGVVLWFENGFTSRFCHENPVILSTSPYTPSTHWSQTIFTFKEPIALSSGKSKANISSPVGTEASPAMKLRLRISIARALEHRSIDIAIETTAIGFEGRKHSLPVQIFNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGI7</accession>
    <name>A0A175YGI7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable 6-phosphogluconolactonase</fullName>
            <ecNumber evidence="5">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0934907</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGI7"/>
    <dbReference type="Gramene" id="KZM82477">
        <property type="protein sequence ID" value="KZM82477"/>
        <property type="gene ID" value="DCAR_030046"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200564"/>
    <dbReference type="OMA" id="ARMIQPA"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000009">
        <property type="entry name" value="Probable 6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF17">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH15369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="267" mass="29253" checksum="42F7D970B1BD31D9" modified="2016-09-07" version="1">MALSGACRDGMEVRIQESVEELSTDLADYISDLSEATIKERGAFCIAISGGSLVSLMRTLISAPYIKTIDWAKWHIFWADERVVAKNHVDSNYRLAKDIFLSKIPLVPSHLHSINDSLTAEKAANEYEFVIRQLVRTRVVGVSEISDCPRFDLILLGMGYDGHVASLFPNHSVLNEKDDWVTYIIDSPKPPPERITFTLPVIKSAANVAVVVTGSNKADIVHMAIDDVGLECPSVPAKMIQPINGKLIWFLDKSAASKLGGASQTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YK21</accession>
    <name>A0A175YK21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_030771</name>
        <name evidence="4" type="ORF">DCAR_0935745</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM83202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YK21"/>
    <dbReference type="Gramene" id="KZM83202">
        <property type="protein sequence ID" value="KZM83202"/>
        <property type="gene ID" value="DCAR_030771"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200670"/>
    <dbReference type="OMA" id="HSFYVAD"/>
    <dbReference type="OrthoDB" id="879807at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000996">
        <property type="entry name" value="Small kernel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF540">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="400"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="536"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM83202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="69659" checksum="84D107FCC027ABE3" modified="2016-09-07" version="1">MITFSALRQLTKFTPTISRNYTSATLQLLQLSITNQSLTQTLQSHARVCYLGLTQHSVIVTKLISAYSLCKNPLAARLVFDSVRVKNVFIWNTLINGYAKNGLCSESFELFREMCRNGDDPDNFTISTMAKVCGDVEDGLLGDLVHGVCVKNGFGVDIVVGNAFIFMYCKCGRFRDAFEVFDEMPERNVSSWNVMIGGVSGLGGDGVSEVVWEFVRCMVVEGFGFDGFTISSLLPFCGRSNGKCDFGRELHSYIVKNDGGFFVESDFYLGCCLIDMYGKSKRVNVGRNVFNCMRDRNIFAWTAMISGYVQNGDFDEAMFLFGEMQSKGRIEPNKVSLISIIPACSSLGGLLGGKQVHGFAVRKELNHEVSLCNALIDMYSKCGSLNYARKVFDFHCISKDAISWSSMISGYGLHGKGSDAVLLYNKMLQSGIKPDFITVVGVLSACGRSEMVEEGLDIYKHINEYGLEPTLEICACIVDMLGRSGQLDKALDFIKTMPLEPGPSIWGSLVNASVLHGNSDMLDLAYKFLIQVEPANPSNYVSLSNLYASSRNWDAVAEVRAKMKERGLKKLPGCSWISVNNETHSFYVADKAHHCSDLIYQMLQELIPVMMGASNSTILEDLALSYLEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKM7</accession>
    <name>A0A175YKM7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0830541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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        <source>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </reference>
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    </dbReference>
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        <property type="gene ID" value="DCAR_028597"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KZM83981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11261.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="164" mass="18707" checksum="A1B29029B121A5DA" modified="2016-09-07" version="1">MILVDKKLITDGIKNGEKYQIYPEDVDISRVPRIFHSSCANGHVFYNFPQDDREQLKMLGQPRSFRGPRVQRHFGDDDESSRGEVSASKRREVYRVVVEANRVTVEDCLKLLEGIDCKSIACSVQKANLFFAELNKDDATRLKGLFCPLSLLSRADQIGENKKK</sequence>
</entry>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KZM84247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YLL5"/>
    <dbReference type="STRING" id="79200.A0A175YLL5"/>
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        <property type="protein sequence ID" value="KZM84247"/>
        <property type="gene ID" value="DCAR_028459"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
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        <property type="entry name" value="TRAFAC_GTPase_members"/>
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        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
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        <location>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF00009"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YLU6</accession>
    <name>A0A175YLU6_DAUCS</name>
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        <name evidence="6" type="ORF">DCAR_028019</name>
        <name evidence="7" type="ORF">DCAR_0831412</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YLU6"/>
    <dbReference type="Gramene" id="KZM84559">
        <property type="protein sequence ID" value="KZM84559"/>
        <property type="gene ID" value="DCAR_028019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sugar phosphate transporter" evidence="5">
        <location>
            <begin position="100"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM84559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH11916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="28003" checksum="AC77C0F1DCC8D529" modified="2016-09-07" version="1">MHRCIPTRQLPILSQLCIFSDAHNPPTLSHPCSSLHRYNPLTKSLTHTLSLSASPSPRFHSLQSSPPKLLAVERRSNSVQVKAAADSITESSDDEALVSKFKDTLLIAILPLAVVHALGNLSTNMSLGKVSVSFTHTIKAMEPFFSVVLSAMFLGEVPNIWIISSLIPIVGGVGLASMTEVSFNWAGFWSAMASNLTNQSRNVLSKKLMVKKESAGLNVNQVYTWSFIAAVCYHAYQQYDHYEHDIWKAEARVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMT6</accession>
    <name>A0A175YMT6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">gibberellin 2beta-dioxygenase</fullName>
            <ecNumber evidence="10">1.14.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_027911</name>
        <name evidence="14" type="ORF">DCAR_0831538</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>gibberellin A1 + 2-oxoglutarate + O2 = gibberellin A8 + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15005"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58524"/>
            <dbReference type="ChEBI" id="CHEBI:58594"/>
            <dbReference type="EC" id="1.14.11.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.13" evidence="10"/>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YMT6"/>
    <dbReference type="STRING" id="79200.A0A175YMT6"/>
    <dbReference type="Gramene" id="KZM84667">
        <property type="protein sequence ID" value="KZM84667"/>
        <property type="gene ID" value="DCAR_027911"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197819"/>
    <dbReference type="OMA" id="KQEDYLY"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000014">
        <property type="entry name" value="Gibberellin 2-beta-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="12">
        <location>
            <begin position="169"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM84667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH12040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="318" mass="35664" checksum="A9D7D971CF78E6C7" modified="2016-09-07" version="1">MVVLSQPVEHVNFPAIKSCNGNSTHIPVNVDLSNPDAKTQIVNACQEFGFFKVINHGVPMEIVTKLEAQALSFFKQPQNHKNKAAPFGYGNKNIGRKGDTGWVEYLLFGTDTQLISQNSLTILPNDFWAMVNKYLSAVKNLACEILKLMAEELNLQPKNVLSRLLSDKKSDSFFRINHYPASDRNELGFGEHTDPQIISVIRSNNASGLEIALKDGTWTQVPADPSSFFITVDDCLQVMTNGRFRSVKHRVITESLKERLSMIYFGGPPLSEKIAPSTSLMQGEESLYKEFTWGEYKKAAFNTKLAFNRISFFEKSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQN6</accession>
    <name>A0A175YQN6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0832394</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQN6"/>
    <dbReference type="Gramene" id="KZM85418">
        <property type="protein sequence ID" value="KZM85418"/>
        <property type="gene ID" value="DCAR_027160"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198769"/>
    <dbReference type="OrthoDB" id="1904546at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05489">
        <property type="entry name" value="xylanase_inhibitor_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000045">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033868">
        <property type="entry name" value="Xylanase_inhibitor_I-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF103">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH12885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="424" mass="44920" checksum="0A9FC701CFE3F0C6" modified="2016-09-07" version="1">MATSSIFIHFAVLYSLVFISSAQTPIRPTGLLLPVTTDRSTLQYTTQIQQRTPLVPVKLTVDLGAPFLWVDCDKSYTTSSYKPARCNSAQCSLAKSTSCMTECYSPAKPGCTNNTCTLFPENTITSSGTSGALGSDVVAVPSANGASVSVSQFLFVCGSTDMLNKLSSGVTGMAGLGATKVSLPPQFVSAFTLKNKFGVCLSSTSSNGAIFFGSFDTSTSPLTKTPILVNPVSTAGISTEGEASSDYFIGVKSININEKPIKIKASLLEINATDGYGGTKISTVQPYTVLERSIYKAVVNAFVKELNVSTVASVAPFGTCFRSKDIGTLYTGPDVPKIDLVMQNKDVYWRIFGGNSMVSVSNDVMCLGFVDGGKNSRTSIVIGGRQVEENLLEFDVASSTLGFSPLLFRKQICANYNFVVTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YR30</accession>
    <name>A0A175YR30_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0832689</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YR30"/>
    <dbReference type="Gramene" id="KZM85690">
        <property type="protein sequence ID" value="KZM85690"/>
        <property type="gene ID" value="DCAR_026888"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198938"/>
    <dbReference type="OMA" id="TEVFVII"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF460">
        <property type="entry name" value="SUGAR TRANSPORT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH13180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="543" mass="59436" checksum="BABC042F47D62C7E" modified="2016-09-07" version="1">MAGGGFVAESSVKQYEGRVTAYVIISCIVAATGGLMFGYDIGISGGVTSMEEFLKKFFPSVYRKSEASSSNTNQYCKFDSQLLTLFTSSLYLAALVASFAASASNKLFGRKITMLVGGIIFLVGAFINGFAQDVAMLIIGRILLGVGVGFANTTVPVYLSEMAPPKIRGALNICFQMAITVGILIANLLNYGFEKMKHNGWRWSLGLAAAPALIMTFGAIFLPDTPNSFIQRGKHDKAKHVLQSIRGTQNVDEEFQDLLEASEVARQIKDPWKNIRKRQYRPQLIITLLIPFFQQVTGINVIMFYAPVLFKTIGFGDDSSLMAAVITGVVNVFSTLVSIFTVDKFGRRFLFLEGGGQMLICQITVALLIASQFGVSGRGDFSKPVGNATIAFICLYVAGFAWSWGPLGWLVPSEIFPLEIRSAGQSINVSVNMFFTFVIGQVFLTLLCKLKYGLFFFFGGFVVIMTIFIFYFVPETKNVPIEEMNRVWKAHWFWHRFIPDDAVGLAHDTDHVQNGNGSGSGTSNGRSNGSDKGKDVELASSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YR54</accession>
    <name>A0A175YR54_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mediator of RNA polymerase II transcription subunit 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_026977</name>
        <name evidence="3" type="ORF">DCAR_0832597</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YR54"/>
    <dbReference type="STRING" id="79200.A0A175YR54"/>
    <dbReference type="Gramene" id="KZM85601">
        <property type="protein sequence ID" value="KZM85601"/>
        <property type="gene ID" value="DCAR_026977"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197778"/>
    <dbReference type="OMA" id="ENFKQHW"/>
    <dbReference type="OrthoDB" id="782223at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033244">
        <property type="entry name" value="MED32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35989">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35989:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM85601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH13088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15402" checksum="0D30D8DBC5860547" modified="2016-09-07" version="1">MDSIVDTLNTAYQEYVAAAAGALEAKELAGAQNTPATDAALENFKQRWELFRVACDRAEEFIESVKQRIGSECLVDEATGSVTGKTGQSAAPGLPPISAVRLEQMSKAVRWLVIELQQGSGTAGSSSLTHPSAPFDARFSEDPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3F4</accession>
    <name>A0AAF0W3F4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF8003 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0100517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202945"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058316">
        <property type="entry name" value="DUF8003"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513:SF2">
        <property type="entry name" value="MRAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26010">
        <property type="entry name" value="DUF8003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041948220" description="DUF8003 domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="865"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1282"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1310"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1345"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8003" evidence="4">
        <location>
            <begin position="785"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG81371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1439" mass="154553" checksum="B9F222B7B5EAE122" modified="2024-05-29" version="1">MHPVWTINRLCWCVSAGYFFLLAVSLSSAQYGGIPAESQFNNSESRSWKYLSPSNGSVSCESLEGVGSFDTTCVLNADLYINTDLYVFGHGNLEILPRLSIICPLEGCVLSFNLSGNIKVGEYAAIVAGSVILSAARLSFGPNSLINTTSLGGSPPSQTSGTPSSYEGAGGGHGGRGASCLKSNQTKYWGGDVYAWSSLSEPWSYGSKGGGTSDTHQFGGSGGGRVKLVVKEILYLNGSVMAEGGDGGSEGGGGSGGSIIVHAKKLQGFGTISASGGRGWAGGGGGRISVYGYSNQEAVKVTVHGGLSLGCPSNGGAAGTYFDASVLSLRVGNDNITTETDTPLLDFSTSPLWSNVFVENYAKALVPLLWTRVQVRGQISLLCGGILIFGLSDYQVSEFELVAEELSMSDSIIKVYGEFRVAVKMLLMWNSKITINDSGDTIDASSVLEVRNLAVLRGNSVVSSNSNLAVYGQGLLSLTGQGDVIKAQRLSLSLFYNVTIGPGSLLQAPLDDENSKIEVTKSHCGSQSCPKDLITPPDDCHLNYTPSFSLQICRVEDILVYGIIKGSIIHIHRAATVIVDNDGTITASELGCRNGIGKGNYLNGASGGAGHGGRGGSGIYNGKMSGGGDTYGSPNLPCELGSGSEGPHASYGHVAGGGMIVIGSGQWPLSKLYISGSMRADGQSYDKAVENINGSTVGGLGGGSGGTILLFLQALALVQDSHLSVAGGHGVPFGGGGGGGGRVHFHWSNIDVGDEYVPIATINGTISSRGGTGDSGGLPGEEGTVTGKKCPKGLYGTYCTECPVGTYKDVEGSDASLCTPCSVEYLPSRASFVYVRGGGTQSLCPYRCISDKYRMPNCYKPFEELIYTFGGPWSFALLLSSVVVFLALSLSTVRVKLVGSSSSYDNANSVERLSYNHFPHLLSLSEVQGTRADETQTHVHRMYFMGPNTFREPWHLPYSPPSSVIDIVYEDSFNRFIDEINSFAAYNWWEGSVHSILTVLAYPCAWSWKQWRRRNKVQFLEEYVKSEYDHSCLRSCRSRALYKGMKLGATPDFMVAYIDFFLGGDEKRRDMVTIIQKRFPMCIIFGGDGSYMSPYTLHSDALLTNLLAQHVPPSVWNRFVAGLNAQLRTVRRGWIHSALIHVINWIQSHGNSQLEFHGVKIELGWFQATASGYYQLGILVVVGDYSPHSLHQSDFLECSEDCPSPSSQKVAPFVQKSFLQPQASQLYTNHALCRKKITGGTNGGLINDATLKSLDVRKDFLFPLSLLFHNTRPVGRQDTLQLLISIILLGDIFVTLLLLLQSFWISLATFLAILLVLPLSLLFPFPAGLSALLSKGPRRASLARVYALWNATSLSNIVVAFVCGLIQYGISSSQPPEEADMWNSMSGEDEKWWLLPATLMLFKLIQARLTDWHIANLEVQDFSLYCPDPDAFWAYEPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W602</accession>
    <name>A0AAF0W602_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal peptide peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0102147</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W602"/>
    <dbReference type="KEGG" id="dcr:108227402"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38688" checksum="0DC57745E6F6C056" modified="2024-05-29" version="1">MKNHERAANLALLGLTLAPLIMKVDPNLNVILTASLTVYVGCYRSVKPTPPTETMSKEHAMRFPLVGSAMLLSLFLLFKFFSKDLVNAVLTGYFFILGIVALSATLLPSIARFLPKHWNEDVIKWRFPYFSSLEVEFTRSQVIAAIPGTFFCAWYVSKKHWLANNFLGLAFCIQGIEMLSLGSFKTGAILLGGLFFYDIFWVFFTPVMVSVAKSFDAPIKLLFPTADAARPFSMLGLGDIVIPGIFVALALRFDVARAKESQYFKSAFLGYAVGLVLTIIVMNWFQAAQPALLYIVPCVVGFLAFHCIWNGEVKQLLAFDESKHSDSQDNDDSQDKDDDPKVSKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W989</accession>
    <name>A0AAF0W989_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0103989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W989"/>
    <dbReference type="KEGG" id="dcr:108210066"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000031">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG84804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="249" mass="27257" checksum="A4C1900E003103E7" modified="2024-05-29" version="1">MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGSAIGILSKDGVVLVGEKKVTSKLLQTSTSTEKMYKIDDHVACAVAGIMSDANILINTARVQAQRYTLSYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKAAAVGANNQAAQSMLKQDYKDDITREEAVQLALKVLSKTMDSTSLTSEKLELAEVFMSSGKVKYQVCSPEALSKLLVKYGVTQPPAEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WB81</accession>
    <name>A0AAF0WB81_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRISC and BRCA1-A complex member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mediator of RAP80 interactions and targeting subunit of 40 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">New component of the BRCA1-A complex</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0104807</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BABAM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WB81"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21502">
        <property type="entry name" value="vWA_BABAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026126">
        <property type="entry name" value="BABAM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660:SF1">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG85616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="256" mass="28688" checksum="C06922474FAD1D03" modified="2024-05-29" version="1">MEASSGEYTLPPSTYSSEDIIFCIDTHNECLREMKAKGAEGQAFTRLDAIKQSILLFITSKLTINSDHRFAFCALGNSPFWLQKEFSNTPESALASFRKITPHSSSDQADLTQLFRAAAHEAKRSRAQNRILRLILIYCRSSVPPHHKWPATQKLFTLDAMYLHDKPGAENCPQKVYDALVDTIEEVSEYEGYVFESGEGLPRILFRNMCVLLSHPQQRCIQDDIDIPKSLPKKSAAVEITAGGENVPSSSQELSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCA9</accession>
    <name>A0AAF0WCA9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0205693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="301"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="42762" checksum="97F870EAEC660BDE" modified="2024-05-29" version="1">MAGRPDVATEHVNSRIKIAAVSRPLFQKKIATSNCDLIPDTLYAAAIAGDADAIAQLATKADRLNLKGETILHTESRNGNAEHVRFILREFADKNLLSKLCESKYTALSTAIDHGRTEVAGILIDAARQWPINSFQDFLRLGNYWMDTALHIAVTRKNVDVVKLLVEADPSDTHTHNKAGETPMYIAVKAGFNDIAEIISTTCSAPYLYGRYGSSAVLIKNVDHADSSGGTLYEIMNKDALYVAAIAGNAGARAALEMQADKVDNVQPILQVESSEGNTEHVRFILSEFANKNLLTKLNSINETALHWAAYKGHTEVAEILIDAASRLPPSDDDSQVTSSQAFLRQADKHLRTALHNAVMKDNVAIVKLLLEADPSDTHSKNDWHFTLKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDK5</accession>
    <name>A0AAF0WDK5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0206811</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDK5"/>
    <dbReference type="KEGG" id="dcr:108209722"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
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        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF231">
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    </dbReference>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1"/>
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    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG87582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="475" mass="52487" checksum="DA6ACE6BC94000A0" modified="2024-05-29" version="1">MLSPEDLSSSLSGFIQEPLPISNPIPNPISGPATAKRKRSLPGTPDPDAEVIALSPASLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNTKEVIRKKVYLCPEKTCVHHHPSRALGDLTGVKKHYSRKHGEKKWKCDKCSKKYAVQSDWKAHSKICGTREYKCDCGTLFSRKDSFITHRAFCDALAEETASRVPAPNTLHLRNELMMNMNNLQSNGSAQFSGMFGSVDDQTGQLNLDGNKSRLPLWLDHNPNAFLASNSGGLAPELVQQIPNNMYGLVLKEEQDTGGNMSSMYYANNINTTPTHHHQEASQSHMSATALLQKAATMGSTRSNSPGMLANSFGLMNSTMSSNAHNFSSLNQGAIMLMGDHHNNAARASDRFMMMMGEKQIHENSMNYNQLGGGLTRDFLGVGENNSNQNIFNSGSNINNSELESGRLFLQQELQAKFGSMSSTMDLAEQYSCTTTGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WI54</accession>
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            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal-dependent phosphohydrolase HD domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF34">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="73"/>
            <end position="211"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="468" mass="54664" checksum="0D404ED4E3136670" modified="2024-05-29" version="1">MGAINNDDYPRYLYASSMADRRVSKQVHDNVHGNIYLDPLSLKFVDTEQFQRLRDLKQLGLTYLVYPGAVHSRFEHSLGVYWLAGETIHKIKIDQGSELGIDHFDIQTVKLAGLLHDVGHGPFSHMFEKEFLPKVRHNHNWSHEQMSLKMIDYIVDEHNIEIDPDILKKLKEMIVSSSEDTSSTSLKEKRFLYDIVANGRNGIDVDKFDYIVRDSRACGLTCCFRFDRPLDTMRVIGDEICYRAKEYLTIYKLFSSRADLHRTVYTHAKVKAIELMFVDALTKAHNHLDILSCIDDPAEYWKLDDSIMKHIETTRSEELKESRDLISRIRRRDLYQFCNEFTVPKDQLEYFKDVTPQDIICSQKAGVTLKEEDIAVSNIKIDMTRGRKNPLESINFFKDYECNEKFPIPDERISLLLPECFQDKIVRVYSKKPELVEIVSEAFENFQVKNYGMKTQVHGTPQKKRQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WIM1</accession>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L16"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="rplP_bact"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG90349.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13218" checksum="E089EC18D72B8B9B" modified="2024-05-29" version="1">MTGIAHQGNHIAFGTYALQALDHSWITSRQIEAGRKVLVQNARRTGKIWVRIFPSKPVTIRPTETRMGRGKGAVAFWVAVVQPGRIIYEISGVTEQMAKEAITIASSKMPVRTKFISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ51</accession>
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            <fullName evidence="3">Alpha-carbonic anhydrase domain-containing protein</fullName>
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    </protein>
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    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>campanulids</taxon>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJ51"/>
    <dbReference type="KEGG" id="dcr:108208561"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF236">
        <property type="entry name" value="ALPHA CARBONIC ANHYDRASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041926545" description="Alpha-carbonic anhydrase domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
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        <location>
            <begin position="30"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="272" mass="30064" checksum="76557F00D9274CAC" modified="2024-05-29" version="1">MASFRFSVLVITISLVIVTAYSTLDADETIEFGYVGPKGPDKWGSLDPKFAVCADGKAQSPIDISRTKATPNNQLRALSRDYYSAGAKLVNNGFNIAVHFDSSAGTMNIDGKNYSLLQLHWHTPSEHTMDGKQLDAELQLVHKADDGSMSITGILYQVGNGDSFLGRVQKHLKELAKDKCGPNEHSEIPLGNLSTRQLKRSTRNYFRYTGSFTTPPCTENVTWIIFGKPSSMSQQQIDALKAPMEEAYKMNARPVQPDNGRPVNLHDEPKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMI9</accession>
    <name>A0AAF0WMI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0311094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMI9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG91839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="474" mass="52644" checksum="04C24787F86EE685" modified="2024-05-29" version="1">MMRVELIFMPVPEVGHLVATAELAKLLVGRDERISVRILIQKMPYDTGFAGFTQKLKKDVPERIDFVDIPAPDETTMAELKALPLTSFHEAFVSNQRTVVRDIVTAILKQSETSKLGGFVLDLFTTPMIEVANDFNVPAYVFFTSSAAYLNIAFYAQDLKDSKNLEISGFKNLDIELSVPGFSNLVPAKGLPAVILDEAGSALMRTIARRLRKTKAIFLNTVLELEARAIKALEEDGNTPVIYHVGPLINSEIGQPTSQNKISNEAIMSWLDRQPCTLSYPDTVKEIACALELSGHHFLWSLRCPSQTKEKRDLPKDYEDYNEVLPEGFLERTSGRGKVIGWAPQMTILSHPAVGGFVSHCGWNSTLESVWCGVPIATWPIYAEQQTNAFQLVKELGLAVEITLDYRKDLIGDVSPTVTAQEIELGIRRLMNAGSEMRNKVKAMKDLCRKANNEGGSSYSSLGQFIEEVIDNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN80</accession>
    <name>A0AAF0WN80_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0311949</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN80"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000134">
        <property type="entry name" value="Casein kinase I isoform delta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG92674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="463" mass="52497" checksum="D81210E997C24016" modified="2024-05-29" version="1">MEPRVGNKFRLGRKIGSGSFGEIYLGTINQTKEEVAIKLENIKTKHPQLLYESKLYRILQGGTGIPNLRWFGVEGNYNILVIDLLGPSLEDLFNFCSRKLSLKSVLMLADQMINRIEFLHSKSFLHRDIKPDNFLMGLQRRANQVYMIDFGLAKKYRDTSTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYLLMYFLRGSLPWQGLKAGNKKKKYERISEKKVSTSIEALCGGYPAEFSSYFHYCRSLRFDDKPDYAYLKGIFRDLFIREGFQFDYVFDWTILKYQQAEISVPPSRGLGRTAGPSSAIPPATPYINRQTVKQVGLSSTDVSDRICAQDKPIDSLTKQKAPVENHSIPNRDAILPGTTFSERLTRQSGIFTGEAFPGGNELDNPYSCITEASPGKLHKISGEQRGSFNPRHASSGKGHSNLMNYETTLRGIDSLHFGETKRGQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNP4</accession>
    <name>A0AAF0WNP4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNP4"/>
    <dbReference type="KEGG" id="dcr:108214047"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000059">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTM1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="22"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG93880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="348" mass="37126" checksum="43D926A5D8C65CCC" modified="2024-05-29" version="1">MANFADEHPVKSFGWAARDTSGHLSPFNFSRRATGEKDVRIKVLYCGICHTDIQNATNGWGASTYPVVPGHEVVGEVTEVGSKVEKVKVGDKVGVGYIVGSCRSCDDCASDLESYCPQQKLTCGGVYYDGTLTFGGYSDSLNLPLDAGAPLLCGGITTYSPLRYFGLDKPGMHIGIVGLGGLGHIAVKFAKAFGTKVTVISTSLAKKQEAIEHLGADSFLVSRNPEEMQAAAGTMDGILDTVSAVHPLQPLLMLLKSHGKLVLVGVPTKALELPVYPLVQGRRVIGGSGTGGMKETQEMIDFAAKNNIIADVEVVAMDYVNTAYERLEKADVRYRFVLDIGNTLTKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVB9</accession>
    <name>A0AAF0WVB9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYB transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0414865</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVB9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000168">
        <property type="entry name" value="Telomere repeat-binding factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044597">
        <property type="entry name" value="SMH1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267">
        <property type="entry name" value="SINGLE MYB HISTONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267:SF8">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="11">
        <location>
            <begin position="73"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG95541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="178" mass="20322" checksum="F43424C9FE190173" modified="2024-05-29" version="1">MGAPKQKWTSEEEAALKAGVKKHGAGKWRTILKDPEFSGDKLRNMSVIANGWGSGEKARLALKTMHQSSRDDDNSVVFVYRILIGQSYYESNNQLEPVGSNKIIIGTYIEKQYWAPPNFKRLLSAKLKFLTNTGRLIKIKRYKIASTSVSSDKRKQQPSMLLLERKQRSYKLMMNSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVF7</accession>
    <name>A0AAF0WVF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0416152</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVF7"/>
    <dbReference type="KEGG" id="dcr:108219584"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:spindle pole"/>
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        <property type="term" value="F:gamma-tubulin binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GCP"/>
    </dbReference>
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        <property type="entry name" value="GCP_C"/>
    </dbReference>
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        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
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    </dbReference>
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        <property type="entry name" value="GCP_N_terminal"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="60"/>
            <end position="351"/>
        </location>
    </feature>
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        <location>
            <begin position="354"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG96815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="699" mass="79374" checksum="BFDA67602180B861" modified="2024-05-29" version="1">MESTPSWNVDRPFLTGRFHQETKSASGLCESKGFSMETFSPGLDKAIACYHASLQEMIVIDDLLSCLVGIEGRYISIKRVKGNVDSISFQIDASMDLALQESAKRIFPLCESYLLIYQFVESMSQFKAGLVNHAFAAALRALLLDYQAMVAQLEHQFRLGRLSIQGLWFYCQPMMGSMQALSIVIKKASAKSIVGSAVLNLLQSQAKAMAGDHVVRSLLEKMIQSASSAYLVILERWLYEGVIDDPHGEFFIAEDKSLQKESLSQDYDAKYWRQRYSLKDEIPSFLASAAETILTTGKYLNVMRECGHTIQVPMSENTKLMSSGSSHHYLECIKAAYEFSSSELLNLIKEKYDLMGKLRSIKHYLLLDQGDFLVHFMDIARDELVKRPDEISVEKLQSLLELALRTTAAAADPFHEDLTCCVERTTLLKRLSTLKDLKFIGTVPEKDDVDEPVNITGMDTYSLSYKVQWPLSLVISRKALTKYQLIFHFLFHCKHVNRQLCEAWQIHQGIRALNMKGTAVSVSSLLCRSMLKFINSLLHYLTFEVLEPNWHVMHNRLQGAKSIDEVIQYHDFFLDKCLRECLLLSPVLLKKMERLKSICLQYAAVTQWLIASSIDIPKTDAQSNTSHETKKSKNVKRRNPSQKLKLASEDKTLVEPILKFEKEFTNELRSLRPILSSSAQAEPYLTHLAQLILGVGRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVV5</accession>
    <name>A0AAF0WVV5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
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    </dbReference>
    <dbReference type="InterPro" id="IPR053264">
        <property type="entry name" value="Lipoate-ligase_2_inactive"/>
    </dbReference>
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        <property type="entry name" value="BIOTIN/LIPOATE A/B PROTEIN LIGASE FAMILY"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43506:SF1">
        <property type="entry name" value="BPL_LPL CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="32"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="250" mass="28542" checksum="CC790F12BF703AD8" modified="2024-05-29" version="1">MAALPLMKLIRLKGVPILQQLHLEERLLRTSPHNWCIINDGTNLPHIVMGISGKPAELLEINSVLRDKVPVIRRFSGGGTVIVDTGTIFVTFICNKDDVSEVQPYPRPIMSWSSLLYSRVFQGVGDFVLRENDYAFGNRKFGGNAQSITKNRWIHHTSFLWDYEINNMAYLKLPKRAPEYRLARNHLDFICCMKEYMPRTNFITRTVDALQGEFSVETTDLEAVESPSNTKFIHSSKILTERELEEAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1F4</accession>
    <name>A0AAF0X1F4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0418267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X1F4"/>
    <dbReference type="KEGG" id="dcr:108216506"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000294">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g09900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002041">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g09900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="427"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="462"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="497"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="532"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="567"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG98921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="68286" checksum="AE4526F2B80D2018" modified="2024-05-29" version="1">MDLMVPIQQSREGFCLFHSFCKDSDIKIQTLRCKDRVRVCFSGGVKVFDGEKFDSGGNGDGLGRNEVFSEKRRNEVSAVSRSDNFGLNGEGANFDVRNGGGLSLSKYCCSRNFEEYESNNCLRRLVRDGELEEGFRCLERMVYQGDVPDIITCTSLIKNFCRVRKTKKAARVLEILEESGAVPDVITYNVLISGYCKVGEINSALEVLDRMSVAPDVVTYNTILRSLCDRGKLNQAMEVLDRQLQKECYPDVITYTILIEATCKESGVDQAMKLLDEMSSNGCKPDVVTYNVLINGICKEERLDEAITFLDNMVSLYGCQPNVITYNIVLFSMCNTGKWTDAEKLLTEMLRKGCSPSVVTFNILINFFCRRGLLGRAIDILDKMPEYGCTPNSLSYNPLLHWFCKEKKLDRAIEYLHVMVSRGCYPDIVTYNTLLTALCKDGKVGIAVEILNQLSRKGCPPVLITYNTVIDGLTKVGKTKFAIELLGEMRGKGLQPDIITYSSLVGGLSREGKIEEAVNFFHDLEGLGVRPNAITYNNVMLGLCKAHRTDRAIDFLINMVAKGCKPTVGTYTILIEGLAYEGLVKEALEILNELCSRGAVKRRSARQMAAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X2F7</accession>
    <name>A0AAF0X2F7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Eukaryotic translation initiation factor NCBP</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Novel cap-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">mRNA cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0519506</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2F7"/>
    <dbReference type="KEGG" id="dcr:108220011"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000008">
        <property type="entry name" value="Eukaryotic translation initiation factor NCBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH00148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="24725" checksum="37B8C3AE9373A21A" modified="2024-05-29" version="1">MELIEKKEGESGDQSSLNNDKEAEERQARDLKAGFHPLKNKFVLWYTRRTPGVRSQTSYEDNIKQIVDFSTVEAFWICYCHLARVSSLPSPTDLHLFKEGIRPLWEDAANCNGGKWIIRFKKVVSGRFWEDLVLALVGGQLDYGDDVCGAVLSVRFSEDILSVWNRNASDNQAVMSLRDAIKRHLKLPHSYVMEYKPHDASLRDNSSYRNTWLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3Z7</accession>
    <name>A0AAF0X3Z7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aquaporin SIP2-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0519370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3Z7"/>
    <dbReference type="KEGG" id="dcr:108223187"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044226">
        <property type="entry name" value="SIP2-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47720">
        <property type="entry name" value="AQUAPORIN SIP2-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47720:SF1">
        <property type="entry name" value="AQUAPORIN SIP2-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH00014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="25856" checksum="8F1D74143DF5228A" modified="2024-05-29" version="1">MGSKIKLLASDMIMSLMWVSSSVLIKRLVFNVLGFGSDAKGEVFKCGVSILNMFFFAWLSKVTKGGAYNPLTVLSPVFSGGFSTFLFTVGARIPAQVIGSIAGVRLMLDTFPEIGRGPRLNVDIHKGALTEGLLTFAIVSISLGLTVKLPGSFYMKTWISSFSKLTLHILGSDLTGGCMNPASVMGWAYARGDHITKEHIFVYWLAPVEATLLAVWTFSLIFRSQKGEKAKVKAEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7K4</accession>
    <name>A0AAF0X7K4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0522285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9G1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="167"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="683" mass="78294" checksum="F94DF6EE897318D5" modified="2024-05-29" version="1">MQDLEEEEGREVEHAEVREKLLELYHRRDSMLWQKARLNWITLGDGNSKFFHQAIQKRRNANNILRILCNNEWVDNPESIRKVFFNHYSNFFKDNHVSLFSLGDLKLSRLSEEGKRDLIREFTSKEIEAAVNSLAADKAPGPDGYNVRCIKYLWPFLKQKIEKFIQDFGESLVLPRGVNSSFIALIPKVTNPVSVKDYRPINLINSSMKILMKLLSSRLSQYMNVLIGDLQSGFMKGRQAAESIMVVKEIAHSIQAGRGKGLILKLDFEKAFDSVNWGFLLEVLEAMNFDPKWISWIKKLLETMRISVLVNGVPTKEFSPGKGLRQGDPLSPLLFNIVGEVLSAMFSKAAKEQIFRGFILDKGTEELTHLQFADDTVVFMDGSIESATGIKRVLQCFQLISGLKINYEKCEIFSSSAAETDLNLCAAILGCKIGSWPMKYLGLPIGISSRRVAFWNPLLQKFSMRLASWKSEALNQAGSLTLVKAVLDSLLVYWMSLHYIPVAVVKKMEKLRRDFLWGKIGTRYQHGRALHSIGWKKICKDKNVGGLGLSTVRAKNVALLGKWYYRWNCDRNRTWNCWIRDKYKLAKSVGLEEACGIKKASEGMKAIMKVSECSSLKDKISRRNFRWRVKNGRMALFWEDWWCGNTPLREVFKQLFHLSKLKEATVSDFLSYCKNGQDMSEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9R4</accession>
    <name>A0AAF0X9R4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0623471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9R4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF517">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH04065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27031" checksum="573AAF5A4F5FB00F" modified="2024-05-29" version="1">MTRDCEMKTPFIQKLLYVVTDCSPGNHKKTLFMVGETGNNDYTSALFQGRPVEELKHKLMPRIVQSIMDAVRKVISIGAERIIVPGQFPLGCFPVYLTEFPSNSTTAYNKHKCLIALNDLAEAHNNYLQHAISTLQTQKPNTKIVYGDYYNAFEWLLNNAPHLGTRSLLSLRNQSTLKACCGTGGKYNLNSTQRCGSPSVPVCPDPDRHISWDGVHLTQKANRLIATWLVADFLPMIDCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XA94</accession>
    <name>A0AAF0XA94_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0622769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XA94"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF232">
        <property type="entry name" value="CYTOCHROME P450 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH03372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56983" checksum="2D3E6DCE400BF4AB" modified="2024-05-29" version="1">MAEIYSNIWFLMILTLVFFIFLVKLLKHRKNFENFGYSKLPPGGRGLPIVGDSINWYNAVSSSHPPSFVKEQVKRYGKIFSCNLLGKRAVVSADPSFNRFIMQNEGKLFQSSYPKSFRNLVGINGVINAQGEQQKKLHSIASNMMRLDKFSFHFMKDIQLVLHQTVTNFENNEVVILQDVCRKVAINLMVNQLLGVSSDAEVDEMAHLFSDFVDGCLSVPINLPGFAFRTAMKAREKIIDKFDKIVKKHREERKSGAELVGNGLLGRLVDDESLADDTMADFVINLLFAGNETTAKTMLFAIYFLTQCPEAMKQLLEEQQALKSQKNGSDAEDEMLTWQDYKAMPFTQCVIDETLRLGGIAIWLMREAKQDIEYQDYVIPKGCFVVPFLSAVHLDENIYEDSLLFNPWRWMEPQNMEKRNWRSSPFYTPFGGGSRFCPGAELARLQIALFLHHFVTTYRFIFISKASFHTHKHTQLILIGSKSQTRLCYSTVLLSIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAD4</accession>
    <name>A0AAF0XAD4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0622675</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAD4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF68">
        <property type="entry name" value="BASIC 7S GLOBULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Xylanase inhibitor N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanase inhibitor C-terminal" evidence="5">
        <location>
            <begin position="86"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="27910" checksum="66AFA1B15D32275B" modified="2024-05-29" version="1">MAGIGNGITSLHAQLSSQFKVPHKFALCLPSTSENAPGHMFVGGGPYYFSPYGKDIAKQLITTPLVENPLKIRGVTQSGEDAEYDFYVNVTSISVDHKLVSFNASLLSIEKDGFGGTSFSTTSAYTILTSSIYESLVSAFVKAAAFRKMIRVASVAPFGACFDSKSIARSQTGPVVPYIDIGLAGNTHWRFYGANSMVAVNNDVLCLAFVDAPFVRSSIHIGSHQMENYLIEFDLVSKKLGISTSLSFRNTTCSQSRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XBR9</accession>
    <name>A0AAF0XBR9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0623500</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with 1-naphthylphthalamic acid (NPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000155">
        <property type="entry name" value="ATP-binding cassette transporter, subfamily B, member 2, group MDR/PGP protein PpABCB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="486"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1002"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1045"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1126"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1229"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1266"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="361"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="685"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="1007"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1328"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="930"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="943"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="963"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH04094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1574" mass="172859" checksum="FAACD9BDF2E55CA8" modified="2024-05-29" version="1">MAESSYEMDYSSHRRPRHTTPVRSHIPASGMSSFSSSYNFSHLSNARRSRPVVPPTPFASDTDTSWQGELSWQFESTGWRDGRNLGAALSPWTATPMSQTSRIFKQSANDYYLSRTTNAFQNLSHSNYEHSGYGTVPSGRFELQSYIARDPGISSSFVGGMASTSGPSNERKKSRGASGVPIVEGRPRKPVSQAEKDSLSLISFDAIQDVERQIVLDPNSNLDEHQRWLSVSHAYTPDHEAVHGHRDHHQESLHNHGGQRHGSHGISHGHGRHNHMSSGPYDISKEQDYGNHGHNVHDSKQYSTSHHYGRGGDHGYDDNDRKSAYEVEDDDEDEDDVGPPRSVGLFSLFRFSTNFDILLVIFGCVGAFINGGSLPWYSLLFGEFVNKIAKDSKNNNLHLMDDVQRICLLMMGLAAIVVVGAYMEITCWRLAGERSAHRIRTEYLRAVLRQDIAFFDTGINTGDIMHGISSDVAQIQEVMGEKMAHFIHHVFTFFCGYAVGFIKSWKVSLAVLAVTPLTMFCGIAYKAVYVGLTSKEVATYRGAGTVAEQAISSIRTVFSFVAEDKLSAKYAELLENSVPFGKKIGFAKGIGMGVIYLVTYATWALAFWYGSILVAKHEITGGAAIACFFGVNVGGRGLALSLSYFAQFAQGTVAASRVFEVIDRVPEIDPYSPEGRRLSSTRGKIEFRGVSFVYPSRPNILILRNLNLVIPPSRTLALVGASGGGKSTVFALIERFYDPTQGLITLDGHDMKTLQVHWLRSQIGMVGQEPVLFATTILENVMMGRENATKKEATKACVAANAHNFISALPDGYDTQVGDRGTQMSGGQKQRIALARAMIKDPRILLLDEATSALDAESESLVQQAIDKISVGRTTIVIAHRLATVRNAQTIVVMDQGSIVEIGDHHQLMEKEGAYFNLTKLASDALSNSASKSTDAHAGTDFSMHEKSVQDSSRSKQVHEISRSQYMKSTQDASRVEADEQKEGKSNDYRLSEIWSLQRPEFSLLMVGLIFGMFAGAILSVFPFILGQALQVYFDDDEEKLKRDVKYLCLTLVGLGIACIISMTGQQGFCGWAGTKLTQRVRNLLFQSILKQEPGWFDFDENSTGVLVSRLSIDCISFRSVLGDRFSVFLMAVSSAAVGLSVSFAIEWRLAIMAALVTPFTLGASYFSLIINVGPKMDNGSYARASNIASGAVSNIRTVATFSTQQQIVESFDRALSEPQKKSVKRSQILGVALGFSQGAMYAAYTLTLWFGAYLVDKNHANFGEVYKIFLILVLSSFSVGQLAGLAPDTSMASTAIPGVTSVINRMPLIRNDRRKVKRIETSKPFDVEFKMVTFAYPSRPEVIVLRNFNLKVKGGSMVALVGSSGSGKSTVIWMVQRFYDPLQGKVLMGGSDIRELNLKWLRKQTALVGQEPALFAGTIRDNIAFGNPNASWAEIEEAAKEAYIHKFICGLPQSYETEVGESGVQLSGGQKQRLAIARAILKKSKVLLLDEASSALDLESEKHVQEALKKVSERATTIVVAHRLTTIRKADAIAVVREGSVTEFGSHDKLMASHLNGIYASLVRAETEANVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCG3</accession>
    <name>A0AAF0XCG3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCG3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019557">
        <property type="entry name" value="AminoTfrase-like_pln_mobile"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044824">
        <property type="entry name" value="MAIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033">
        <property type="entry name" value="PROTEIN MAIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46033:SF8">
        <property type="entry name" value="PROTEIN MAINTENANCE OF MERISTEMS-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10536">
        <property type="entry name" value="PMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase-like plant mobile" evidence="1">
        <location>
            <begin position="71"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="445" mass="51453" checksum="D0D878AB60EFC576" modified="2024-05-29" version="1">MEDILPGPVSQEVILDNSYHVSDAVWAGVDRGPLECFCGNRSMRSWFLSDAQKEILHAIGFGVFANPRLILQTDARLVSALVERWRPETNTFFMRQGEMTVTLEDVGYILGLPIHGRSLVGDAIDNQKDFFQRNWFEELSTADVDPAHTRGGVRYTWLFETYGADPGPDPERILIHTQAYLFVVVGAVLFPTTSRNMVHPRYLRHLRRLREIPTWSWGSAVLSYLYRGLYHATQKDAKKISCCVWLLMLWSYERFEIGRPIPDESRGSYPVADRAAPHHSLPHYRGEFDGVALDVLSWTPYSEFHEMEGDSDGDHDISAEDWEARYAGLARVPLLHYDIVEYQHSDRVLRQFGLRQPIPPSPVSMTRYRQEKEVFLIQWERFVDHPEVVVAQHSLDADDTGYLEWYEHISRRRVGKPQPVPQPQYSTRELFDNLQAFQLVSLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF61</accession>
    <name>A0AAF0XF61_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caffeoyl-CoA O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0626359</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF61"/>
    <dbReference type="KEGG" id="dcr:108224929"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF14">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31033" checksum="E83FA73A63EADAEB" modified="2024-05-29" version="1">MASSLACRCVNSFPQFRNRLFTTTSLPKPNAVVFLKHIACSSIAAAETLTSDAVVVSGDGKYGRKEIISITPRLYDYLLDNVREPQILRELREETSTMRGSQMQVSPDQAQLLAMLVQILGAEKCIEVGVYTGYSSLAVALVLPESGRLVACERDGKALEVAKRYYEQAGVLHKVDVRHGLAADALKLMIQNGEGCSYDFAFVDADKRNYQEYFELLLQLVRVGGIIVIDNVLWHGKVANPLVNDAKTVSIRNFNQGIMEDERVSISMVPIGDGITICRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XG26</accession>
    <name>A0AAF0XG26_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 25 member 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0726825</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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        </source>
    </evidence>
    <sequence length="488" mass="55204" checksum="55C3CF8E36A11776" modified="2024-05-29" version="1">MQSACTHICNSPLNPLRPHFLGFLPPQFSPPHLNFTPINHSLRCISCANRTPKGTDRLNARGKDNVWSVDIDTDKGRDGRIRKKKRERRVRKVAKKSGGSGKVMVSGSVLMEVETVLQTQEPVIRPAWNTFSSSLNGIWKGVGAIFSPITAEMEPIDVGKKGEHLFDCYTLCHIETVGCTSGEQKSQIKRRVNWVTLNPYGENHQLKGTENRSKEKYVREDSSSQKKVYDANTGNHILPKYESFNFETSDVMEDDVMSMEPGLVFFEDGSYSRGPVNIPVGEVSESEYFLSPTFKFEQCLVKGCHKRLRIAHTIEFSNGGSDIQIMRVAVYEEEWDSPVNLPDLSAIEFDLKPFSQRKRVQPSQLVGFWKVFEMSATPIYGEETIAEEAEGTTPYVYLCTETLKKRNLPDNPVYFGEEEVLDMADVTVLWLPGGITSYVDVNKDGDLCIGVGWYSDEGINLVMERDYGLDGKLKEIRTKSEVKRRWPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRD7</accession>
    <name>A0AAF0XRD7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0831378</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRD7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1551">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH11882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="483" mass="53899" checksum="73448A95F43A81B8" modified="2024-05-29" version="1">MNSEAEAAEKQAHVVCIPYPAQSHMKGMLKMAKLLHSKGALITFVNTEFNQRRFLKSGALESVESLPGFSFQTIPDGLPPSDADATQDIPQLCHSIIQNNLLPAFEDLLAKLNAGIHPVTSILSDGFMPFTADAAHSLGIPVVLLWTIAACGFMGFYQFKNARDRGLVPLKDESYLSNGYLDTIIDWIPGIRDIRLRDLPSHIRIIDPNDIMFNFFLESTQRAKNGTAQVLHTFDDLEQELVDVISSMYTTSVYTIGPYQLLVNQIPLDEKEGLKSIGYSLWEEEKTCLQWLDSKEADSVVYVNFGSITVMSAEQLVEFGWGLANSNFCFLWIIRPDLIVGDSSNTLGVEFMDAIKNRGFISSWCPQEDVLNHVSVGGFLTHGGWNSTIESISAGVPMLCWPFFADQQINCKFMCDKWECGLEIPNNGKRDDVEKLVRLLMEGVEGKKMRRKAIEWKKLAEKACGPNGSSSLNLDKLVLLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XS84</accession>
    <name>A0AAF0XS84_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0831207</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XS84"/>
    <dbReference type="KEGG" id="dcr:108197620"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000058">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="13">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH11716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="394" mass="44541" checksum="A10FB94ED32604D7" modified="2024-05-29" version="1">MYSSNSWSCSKCTFINPISQPPICQICLTPQNPDSHSSRSQSQSQPKWSCKLCTFLNSPGVDVCEICGARALDSLLAEASAGSKRTFEDLLDKGLGDDGGSNATKLAKLDVVHLDETSTNEDLKTLKILSYNVWFREDLEMHKRMKSLGDLIQMHSPELICLQEVTPNIYDIFQRSSWWKLYKCSISSEMAFTRPYFCMQLSKVPVKLFSCKPFQNSIMGRELCVAEVVAEKDKPLIIATSHLESPCPAPPKWDQMYSKERVSQAREAIDSLNKNKNVIFCGDMNWDDKLDGQVPLPDGWIDAWTEHRPGELGYTYDTKTNKMLSGNRTLQKRLDRFLCSLHDYKIDGIEMIGMEAIPGLSYSKEKKVKKEVKNIILPVLPSDHYGLLLTISSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWP2</accession>
    <name>A0AAF0XWP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0933342</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000033">
        <property type="entry name" value="Kinesin-like protein KIN-12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="195"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="276"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH13831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="766" mass="85423" checksum="FA1718C72AD9E411" modified="2024-05-29" version="1">MLRNLRKSSNLEEKENTQVNINIIVDEEVGFKSTKVCKTPLKASKNRNVDAMLPLKTSEKQGVLAENSYGVGQKNVVSSNVFEMGIDSIRYNKNGNGGFANVITPMPSRANGKGSLSNSTQCTPIKSVIRPPNPKLCLTGGGTGAASVGGGARMANFEALSKGVPISSSSSTVVNSIEVPQFGIKEDPSFWMDHNVQVLIRVRPLSGAEKSMFGYNRCLKQESAQCITWIGQPESRFTFDHIACETLDQETLFTAIGLPMVENCLSGYNSCIFAYGQTGSGKTYTMLGDVNNLGDKSNPDRGMTSRIFEFLFARIQAERESRRDERLVYNLKCSFSEIYNEQITDLLDPSSTNLLLREDTRTGIYVENLSEYEVQTVGDILKLLSQGSANRKVAATNMNRESSRSHSVFTCVIESRWENDRDSMPYLRFARLNLVDLAGSERQKTSGAEGDRLKEAANINKSLSTLGHVIMLLGDVVHAKTKHVPYRDSKLTFLLQDSLGGNSKTMIIANVSPSICCAAETLNTLKFAQRAKLIQNNAVVNEDSPEAVTALKYQIHLLKEELLVFKKQNVARSLTFGSTSIGDTRQEIGSACINKELEVGCQNKGGSSTLLIVCLQGELSILQQQVQESYLREIETEDDDLRKEIGGVTKANMRLSSKIAKLKHEYEKSLDDMQVSSNEEGRRLQEALYHIQVLEQEIAAKDREWYMYYGVSILNIEHLQDQEQRHSLVSIDYKCFERLFEGNRKLKLVKLKALLHLFFRSFLFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AEV1</accession>
    <name>A0AAF1AEV1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2 RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100027</name>
        <name evidence="9" type="ORF">DCAR_0100053</name>
        <name evidence="10" type="ORF">DCAR_0100104</name>
        <name evidence="11" type="ORF">DCAR_0100147</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AEV1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000029">
        <property type="entry name" value="50S ribosomal protein L2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="7">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG80882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG80908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG80959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG81002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="15000" checksum="36374B8B095C66A7" modified="2024-05-29" version="1">MAIHLYKTSTPSTRNRTVDSRVKSNPRNNLIYGQHRCGKGRNARGIITAGHRGGGHKRLYRKIDFRRNEKDIYGRIVTIEYDPNRNAYICLIHYGDGEKRYILHPRGAIIGDTIVSGTEVPIKMGNALPLSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALG6</accession>
    <name>A0AAF1ALG6_DAUCS</name>
    <protein>
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        </submittedName>
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        <name evidence="1" type="ORF">DCAR_0206293</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG87073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="5639" checksum="66CA40BA641F9AE1" modified="2024-05-29" version="1">MVGLNTEVAAVGGATVTVKTGSGDWLPVSWFPRAEKVRSQATGACSSGRDLLGSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMD4</accession>
    <name>A0AAF1AMD4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Growth-regulating factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207764</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transcription activator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The QLQ domain and WRC domain may be involved in protein-protein interaction and DNA-binding, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="5"/>
            <end position="49"/>
        </location>
    </feature>
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            <begin position="218"/>
            <end position="262"/>
        </location>
    </feature>
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        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="308" mass="33627" checksum="5C5273B2B3580392" modified="2024-05-29" version="1">MSLMGPETGRCRRTDGKTWRCLKDVVPDQKYCERHMHRGRSTKRVEAFGIKSHNLAVTSAKLSTPISGNCQNKNPSPCRSNDLTKSATSITTLANGKSKPTNRLVKKDKSSRLVTINACNHNISCVGTIIYMSIHLRLQELNFKKMTPFIISSRENTDCLDGVDAGSNQLKDTNKGYSTNGDKHGASVTPELGNSTESVVYGDTIDTNTVCINCAITVEESQRCKRTDGKRWQCSREAIPQEKYCGSHINRGSKRCRLSPEAATAAATLATLVTLKNGPSPTNLNTDLCILHATHPPTVLESSTSGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AN09</accession>
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        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0205332</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="36" mass="3900" checksum="9A51E30953E28F3A" modified="2024-05-29" version="1">MAEAVVSFAMQRLGELLISEANLLHGISDQINENVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQ40</accession>
    <name>A0AAF1AQ40_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DCAR_0310296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG91048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="203"/>
            <end position="224"/>
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            <end position="318"/>
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            <end position="368"/>
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    </feature>
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        <location>
            <begin position="375"/>
            <end position="396"/>
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    </feature>
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        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="30"/>
            <end position="505"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG91048.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="56590" checksum="FBFD25D3AE240977" modified="2024-05-29" version="1">MNKYKVIEKKCPGCEIHKLKYSTTRIPVKHLIFVWVVTLSAALPISSLFTFVYFMIRDFQIAEREEDIGYYAGFLSSSFMFGRALTSVLWGILADRYGRKPVIIFGTISVVIFNTLFGLSTSFWMAVSTRFLLGSLCGILGPMRAYATEVCRREHQSLGLSVISTSWGVGLVIGPAIGGYFAQPAENYPNIFSEKSIFGRFPYFLPCLLISLFAFLVSIISFWLPETLHIHHGNKNEQCDAADALENSMYKYRVDGVSRHGNKNEQCDAADASENSMYKSRVDGVIRSKERLPSSKLSLLRNWPLMSAITVYCVFQLHDTAYAEAFTLWADSPRKYGGLHYKIPEVGEVLAISGFGLLILQMFLYPVLERTFGAILVSRIGAVATIPLLTCYPYIAKLSGNLLFAVLSCASILMNVLMVSVSTGLYLLQNRAVSSDQRGAANGISMSALSLFKAFGPACGGSFFSWAQTRQNPTFLPGDQIVFSILNSIEFIGLAMTFKPFLVLPNDDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQJ2</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Daucus</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="55" mass="6425" checksum="C415B0C9AF008EAB" modified="2024-05-29" version="1">MDVFNSEGSSWADQWDPEPLPNEKKGKNDSSKDKTLKKKLSFEWVRKLVKKSDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVV7</accession>
    <name>A0AAF1AVV7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0313660</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVV7"/>
    <dbReference type="KEGG" id="dcr:108213028"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000285">
        <property type="entry name" value="RING-H2 finger protein ATL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042293691" description="RING-type E3 ubiquitin transferase" evidence="18">
        <location>
            <begin position="17"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="109"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
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    </evidence>
    <sequence length="381" mass="43594" checksum="3D0E71447AAD4791" modified="2024-05-29" version="1">MHRLIVLSMFLIVIKADHTRGIPANDEAVNEFQPSLAIVVGVLGVMFILTFFLLFLAKCCHRQSGSNGENEGRFGESRSGYSGIDKTVIESLPFFRFSALKGSREGLECAVCLSKFRDIEILRLLPKCKHAFHISCVDQWLERHSSCPLCRHRVSSEDHASVTYSGSLRFLRSQSDLRQDSNLELFVQREEDHTQHKSSRFMRGSSLNNTLEVQDREELPIQGTCHKNEAEEEENLHKFNHKIIVAEGVLLKNRWSSVSSSDLMFLNSEMINEASTNRLSSKEAANNLYVISTQDDSIPFARVVENNSSKILEPNEKRSMSEIIVHPRSTRSRSITRGESSGSQNDVNEERQRKLWLPIARRTVEWFANRERRFTQIQSHV</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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        </location>
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            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
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            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="EMBL" id="WOG94845.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="511"/>
            <end position="531"/>
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    </evidence>
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</entry>
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    <accession>A0AAF1B088</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
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        <property type="entry name" value="CSC1-LIKE PROTEIN RXW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="563"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
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        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
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        <location>
            <begin position="111"/>
            <end position="182"/>
        </location>
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        <location>
            <begin position="204"/>
            <end position="357"/>
        </location>
    </feature>
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        <location>
            <begin position="368"/>
            <end position="636"/>
        </location>
    </feature>
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        <location>
            <begin position="735"/>
            <end position="756"/>
        </location>
    </feature>
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        <location>
            <begin position="735"/>
            <end position="748"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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            <dbReference type="EMBL" id="WOH00383.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B106</accession>
    <name>A0AAF1B106_DAUCS</name>
    <protein>
        <recommendedName>
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    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108220682"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106286">
        <property type="term" value="F:(E)-caffeate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF2">
        <property type="entry name" value="BUTYRATE--COA LIGASE AAE11, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="453"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG99735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="64473" checksum="59ED74978D85B214" modified="2024-05-29" version="1">MDELSKCGANYVALTPTTFLKRASMVYANRASVIYANVRFNWLQTYQRCCRLSSSLRSLNITKNDVVSVLAPNTPALYEMHFAVPMAGAVLNAINTRLDANNIATILRHSEAKMLFVDYEYVPVARDALRLLISQNYSCLPLVIVIDDIDSPTGIRLGELEYEQLVRHGDPSYVPEELQDEWDAIALNYTSGTTSAPKGVVYSHRGAFLSTMSLIQGWEMGTEPVYLWSLPMFHCNGWTFTWGVAARGGTNICIRNTTAQEMYSAIAQHKVTHMCCAPVVFTILLEAKPEEKRNISNPVNILTGGAPPPAPLLEKLERLGFHVMHAYGLTEATGPALVCEWQAKWNILPRQEQAKLKARQGVSILTLADVDVKDLSTMQSVPHDGRTMGEIVLRGSSIMKGYFKDKVATQKAFKQGWFLTGDVGVIYPDGYLEIKDRSKDVIISGGENISSVELESILYKHPSVIEAAVVAMPHPRWGESPCAFVVAKTTDKNKLQEEDLLKHCHQNLPKYMVPKKIQFLNEIPKTATGKPRKTELREIAKTLVISEKPAAKKPTNKGNSRPNYNGHETHDQVLAMSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B320</accession>
    <name>A0AAF1B320_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0521525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B320"/>
    <dbReference type="KEGG" id="dcr:108220100"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37720">
        <property type="entry name" value="OS10G0481400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37720:SF2">
        <property type="entry name" value="OS10G0481400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH02137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="85" mass="9636" checksum="0C328AD973A58006" modified="2024-05-29" version="1">MISVINQERLLGAALGALFTGALVFEQRRRIYKSISENEAQFASLTQTKEPIFEKRSRLEFAHLWNKAVDHTFGPVIESLSSRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B584</accession>
    <name>A0AAF1B584_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0623050</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B584"/>
    <dbReference type="KEGG" id="dcr:108225477"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000433">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000561">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At2g16250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000448">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041911106" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="521"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="849"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="855"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="869"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="899" mass="98793" checksum="5438AB6F234152C7" modified="2024-05-29" version="1">MLIFTRFRLLILILYVLFVSTFQQYQVVPLSSYTERVSLLQLRSSLGIRNKQWPLKSEPCANWVGVKCLNGSVVGINISGFRRTRIGSQNPHFSVDSIANFTSLGYFNASRFSLPGSIPDWFGMRLSALQVLDLSGCSIVGVIPDTIGNMTSLSVLNLSDNGLTGVVPESLSQLSNLSLLDLSRNSLVGSFPASFGSLRNLTLLDMSMNNLSGFIPPYLGTLTKLQILNLSHNGITSLVPPQLGDLSSLVALDLSFNSFSGSLGLDLRVLTNLRKMVIGNNRLSGSLPSNLFSELTQLQLIVLSYNDFSGEFPDILWSMPSVRFLDASGNKFTGIFPNSSSAVTASPAVFNLSQNMFYGDLPYVVRRFRVIDLSNNYFKGKVSEYARNNAVLNRNCFRGESSQRSVAACASFYYKRGLPFHYFGLPVKQPPPLKSDEMSQRDIIIFLGVLGGVGLTAIVIILLIFLILCTRKSQTTNQRATGVGPVATSDAIPAIPGEYLKFSSLGKGFTYQQILQATREFSDLNLIKHGHSGDLFLGALEGGVHIVIKKKLSPVKEAYLPELDFYSKVSHPRFVSLLGHCLDDESEGFLIYKYMLNGDLASNLYRTKSSDGDSLQSLDWITRLKIATGVAEALCYLHHECTPPFVHRNVQASSILLDDKFEVRLGSLSEVCAQEGDMHQSRITRFLQLPQTSKQGSSSGTPTTTCAYDVYCFGKVLLELVTGNMGLSASSEGNMKELLEETLRYISIYDKELLTNIIDPSLIIDEDLLEEVWAMAIVARSCLNPRPAKRPLARYILKALENPLKVVRDESPSSAGHKTVSSRVSWSAGLFGNWRSSSDVPSVPLNKAGGNSFKSSVTQSSQVSVQSSRVDHLSSSKGHQSRDIFPEPLEEDTVRPHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5X3</accession>
    <name>A0AAF1B5X3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0624581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="10">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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        </reaction>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH08480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="378" mass="42155" checksum="BDAA9A21220CEC49" modified="2024-05-29" version="1">MMFMLLKRARKLGQLSYLYCPSCGLSSKASKAFGFVNEGTPSSSVEYKRNVGSGSGSSSFHPFSLLSDSWDNVKDYKIELVDNQTWGVSSGLADAWKGGGQIKDLRNDVVDEFETYVPSNNNDQDFYDIEEMRIRGNLFYKLDKDSKEYEEYNHEFLKKKSSKNKDAQNEKKKEKQSHDSSPIADKSSKNKDGQKDSKMKMNVTDFSVRSGKLDNVNENYHLTSSLADIHGLRDGKKLRTPTFNQLTAAYHEPFCLDIYISKASVRACIVHRATSKVVAVAHSISKDMKFDLGSTRNATACAAVGKVLAQRALADDIHNAVYTPRRGEKLEGKLLVVLESVIKNGVNVKVKIKQRKFTKSGIAPAAKNFNTGSVSRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8M7</accession>
    <name>A0AAF1B8M7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldose 1-epimerase</fullName>
            <ecNumber evidence="5">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0729943</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B8M7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000008">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF3">
        <property type="entry name" value="ALDOSE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041960246" description="Aldose 1-epimerase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="346" mass="38227" checksum="7DBDBF5AF7B8733D" modified="2024-05-29" version="1">MLVFLVFNLLIQLSRGGDIGIYELKKGDFSIKVTNYGATVLSVMLPDRNGKFDDVVLGYDSIKDYSNGTTYFGALVGRVANRIGNARFTLNGVVYHLSANDGNNTLHGGKKGFSKMVWTVEDYQANSHITFTYNSPDGEDGFPGDVSVEVTYMLMDTDKLAIKMKAKPKNKATPINLASHTYWNLAGHNSGDILSHTIQLLASKITPVNKHLIPTGQIVPVAGTAYDFLQAREIGSRISELSDGYDINYVLDGTSKKHFNKVAMVHDSKSGRKMELWTNQPGVQFYTSNMIASVKGKFGFIYHKHAGLCLETQGFPDAVNQPNFPSQIVNPGATYEHIMVYRFTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8P8</accession>
    <name>A0AAF1B8P8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0727453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B8P8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000014">
        <property type="entry name" value="Pyridoxal phosphate homeostasis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="5">
        <location>
            <begin position="27"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH08017.1"/>
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    </evidence>
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            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pectinesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 12"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCM1"/>
    <dbReference type="KEGG" id="dcr:108199289"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000008">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF19">
        <property type="entry name" value="PECTINESTERASE 68-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041784364" description="Pectinesterase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="13">
        <location>
            <begin position="47"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH12202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="347" mass="38342" checksum="FF054BF7401C87FD" modified="2024-05-29" version="1">MSSFKPQFFLIFFVLFSLQTACRSTETFNITRPKHKWVGPSGNLTIHVDLHGSGDFKSVQAAVDSIPNYNQKNVLIKIAAGCYIEKVVVPASKPYITFQGAGRDRTAIEWHDRASDRGPDGQQLRTYQTASVTVFASYFSAKNISFKNTAPAPMPGLEGWQAAAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKDCYIEGSIDFIFGNGRSMYKDCQLHSIATRFGSIAAQDRNCPDEKTGFAFVHCRVTGTGPLYVGRAMGQYSRIVFSYTYFASIVARGGWDDWDHASNKNKTAFFGVYKCYGPGAAAVSGASWARELDYETAHPFLAKSFVNGRHWIAPTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WYE8</accession>
    <name>A0A161WYE8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_012501</name>
        <name evidence="12" type="ORF">DCAR_0314163</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in plant development processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WYE8"/>
    <dbReference type="Gramene" id="KZN03745">
        <property type="protein sequence ID" value="KZN03745"/>
        <property type="gene ID" value="DCAR_012501"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215057"/>
    <dbReference type="OMA" id="KANCVHF"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000788">
        <property type="entry name" value="Serine/threonine-protein kinase Nek3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN03745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG94866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="614" mass="69044" checksum="B65CA71521414141" modified="2016-07-06" version="1">MENYEILEQIGRGSFASALLVRHRHEKKKYVLKKIRLARQTIRTRRSALQEMELISKVQHPYIIEYKDSWVEKGCFVCIVIAYCEGGDMADAIKRTNGVYFTEEKLCKWLVQLLMALDYLHKNHILHRDVKCSNIFLKKNQDLRLGDFGLAKMLTSDDLACSVVGTPSYMCPELLADIPYGSKSDIWSLGCCIYEMAAHKPAFKAFDMQSLIKKINKSIVAPIPVMYSGAFRGLVKSMLRKNPELRPSAADLLRLPYLQPYVLNIHLNSSSPKRHTFPSQQFVTNYVKQTRFSEPEAVTMHSDRENRRSFGYDRALNPSVSGAEQDSLCYSEGSHDSRKFNRKFSIGSIGEDSVSDNTVVTKSISSVKSPIFTPAKVSGTIPRRAATPRIYTTISDHDLCKKIQTPSRKPLPSTRRASLPMSARTLSKTPYKASAELLHDVASPDVSVNTPIIDKMTEFPLASDEDPFFPVRKTSSTSAQCSSTSPSCDNNAITKDKCTVQSFDKSRRTVTRSYITNVDYVAPITRSECSENNPATCISSRPSSESGSQQHRFDTSSYQQRAEALEGLLEFSANLMQQNRFQELSVLLKPFGPEKVSSRETAIWLTKSFKDTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X1P4</accession>
    <name>A0A161X1P4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0417152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X1P4"/>
    <dbReference type="Gramene" id="KZM98807">
        <property type="protein sequence ID" value="KZM98807"/>
        <property type="gene ID" value="DCAR_013831"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218782"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000009">
        <property type="entry name" value="Auxilin-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF70">
        <property type="entry name" value="DNAJ DOMAIN, CHAPERONE J-DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="555"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="782"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1002"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="906"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="788"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1002"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1050"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1201" mass="134720" checksum="0EAF4FB003912716" modified="2016-07-06" version="1">MESSLSTPYHRRRLSGVNGFSSKNAYDDVFSAPPTLGARKVKSRVLEDYKEIFASSRASSIPVLDISPPAGVDADSVSDYRNSELDYSNIFGGLRDDDAGGVCYEQVIANAAGTNRETTKTSRKIRTPSAPESPIHGSDCLKASNESKVLSSDEPVQSVDDKKHFSMSYHKTNTGSKDGTNGTTHVAQFLPVRGFTRFIDETTPQKIEADKSKSSVVSDVLHKVDVNEAILENRNTTKGMSHLTNKDAFRRDSKSRSNSGWDGSYSYDKLFNSHENNLKSHPTENRPPSGIPYKIDSSGNSKRLWASKNDASEHVAGKSSSCIPGEELDVNSAAAASAAVLKDAIQKAQESIRLAKELMERKREGLRRPSSHNSRNNVKVKDKMENVIAYEGNRLKDTNAKDISGSVDFVSQLFSGMRKKSAIRSGLSPDFKQKERSFIKENVVDDENAKSLGMAEESELQSLEKKREVIPSIESMDKVESNVMEQQQVYNEKTDEKAQKLVYVERKLNAVKGDDQTDLNENKVEPVCIVSEPKKVENFRVLQEDEFVEAIHTHIHINKDGEKSSKDSQQEKEDATESESNNDLRVNIVPETEDIENKQDISQQDHIAKTTKDIEKDGSDYGVEKNGDDERISIDIHDGEGSEHIFENFGNEDLSGRFEKAEFICIETEEIEVLQVAHHGSDVYDHVHDKNETEKAFKYNINHVHLETGQEVDDHSTATKDFYDFNVDEAEAVKLAYNPREKEHLSTGNLAPDLADVIDTINNEKVVSEILLSKDSSPKFGSTFTDHGGIESTRKGGVRKDRMPSDEEAGKSTLGYVNDNLQHDAHFEISEGPKAKEKTSEVEVEIKIRKDTTKNVEGVDKIINIKEKGMEMNEEIQARQGKEIHDVSSSKNFDMEKEAKRTVSVVKEKEGLIKNNEEMKRDHEREKEKIAVERAIREVRERAFAEARERAERAAVERATAEVRQRVMAEARDKFDKDTAATKLSAEKASVQAKLRSERAAVERATAEARERALEKALSQKSRAQTERSTSQNHAGPSRDNSTKKSSSSDLETNCENNTDSVQRNKANVEKHERIMERTAKALAEKNMRDLLAQKELAEKNRLAEALDADIKRWSNGKEGNLRALLSTLQYILGPGSGWQPISLTEIITTNAVKKAYRKATLYVHPDKLQQRGANIQQKYICEKVFDLLKAAWNKFNKEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X6V0</accession>
    <name>A0A161X6V0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0728965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X6V0"/>
    <dbReference type="Gramene" id="KZM88143">
        <property type="protein sequence ID" value="KZM88143"/>
        <property type="gene ID" value="DCAR_025218"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195724"/>
    <dbReference type="OMA" id="GVDWDID"/>
    <dbReference type="OrthoDB" id="588983at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1820:FF:000001">
        <property type="entry name" value="Desiccation protectant protein Lea14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045043">
        <property type="entry name" value="Lea14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459:SF19">
        <property type="entry name" value="DESICCATION-RELATED PROTEIN LEA14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="16649" checksum="887AE32F70BC859D" modified="2016-07-06" version="1">MAGFLDKAKEYVTEKVAEMKKPEASVTDVDLKDVSRECITYDAKVSVSNPYSTSIPICEISYKLKSAGREIASGTVPDPGSLKGNDTTMLNVGLKVPHSVLLSLAKDIGADWDIDYELGIDFVFDLPVFGNITIPVSSKGEIKLPTFSDLWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XGF7</accession>
    <name>A0A161XGF7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0207776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XGF7"/>
    <dbReference type="Gramene" id="KZN06049">
        <property type="protein sequence ID" value="KZN06049"/>
        <property type="gene ID" value="DCAR_006886"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209724"/>
    <dbReference type="OMA" id="FFHESCN"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF113">
        <property type="entry name" value="CARBOXYLESTERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="35688" checksum="894807CC7899A5F2" modified="2016-07-06" version="1">MSDQESSNIDPYKFFKISLNPDGSLTRPESFPNVPPTPDTNDLADSQLALSKDIHTKNGILIRLFRPLECPTKLPLIVYFHGGGFVFFSATSAPFHNSCNRMTTVCQALVLSVDYRLAPENRLPAAYDDAMEAIMWVRDQAKHTDGCDPWLKDHADFSRVFLMGSSAGGTMVYYSGLRALDEDLSPMKIRGLIINQGYFGGVERTPSELRLVNDRVVPLAANDLMWSLALPEGVDRDHEYCNPATGSSSEKIQKLPRCLVRGYGGDPLVDRQKELAKMLEARGVQVTASFDEEGAHAVELTDPVKATKLYDEIKNFVHSSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YAR2</accession>
    <name>A0A161YAR2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0622962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YAR2"/>
    <dbReference type="Gramene" id="KZM89579">
        <property type="protein sequence ID" value="KZM89579"/>
        <property type="gene ID" value="DCAR_023058"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225666"/>
    <dbReference type="OrthoDB" id="1928787at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35461">
        <property type="entry name" value="BNAANNG14610D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35461:SF1">
        <property type="entry name" value="LOW PROTEIN: ATP-DEPENDENT RNA HELICASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="156" mass="18335" checksum="F9E60CEF2E1C7A4D" modified="2016-07-06" version="1">MQHSCKLYTESIQKREPGCGELICKKKKKSTYTAHRSVVVHKEHVVVKTIQEVKIKEEEKPARVNEKIRGGFVGQNARERSYILAKKIKELEMLDAYNMDHVFDVQEVLHHYSHLTSPVYLDIVDKFFINMYPDFYLPLPPVHVNKLMRRLNTVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YL56</accession>
    <name>A0A161YL56_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0520055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YL56"/>
    <dbReference type="Gramene" id="KZM94239">
        <property type="protein sequence ID" value="KZM94239"/>
        <property type="gene ID" value="DCAR_017482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="286" mass="32742" checksum="F99E4E4DB1D39241" modified="2016-07-06" version="1">MDNIPYHMISDLEPHIGSQWRLKVWVTRIWQQFNHNGESIGTNMILVDVLFNNLTEVLFDGGVDLIQPHVFHFTNLSNVMATATQDEFLIDVVGILGRVQPINTFINKYNLQQSCIRFTIYDMDTSAKVTLYNEMAESFDQAIHDVVQHPIIVIISSCRAHSFRGQAELTNLAPTRFFINHNHAAVEELRDAISLHQKQMEHNRKNKRRMLAAEKKDESEYSPPKTNSSSSTTSRKMLLPSEKNNEVGESSKTLSDKIDKLIEAIEKLTAAIKKNQRRKFKCSITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZLI9</accession>
    <name>A0A161ZLI9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0100445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZLI9"/>
    <dbReference type="Gramene" id="KZN07986">
        <property type="protein sequence ID" value="KZN07986"/>
        <property type="gene ID" value="DCAR_000655"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214943"/>
    <dbReference type="OMA" id="LLGYMFP"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF3">
        <property type="entry name" value="OS04G0543600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG81299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="577" mass="63190" checksum="2C99683F5D4A6445" modified="2016-07-06" version="1">MEVLNQPAASTNVCFSSYIHSLSETPHRLRNRMLATWTPEQELNQMRLRSGANMKRKLMWYDLVALGVGGMLGAGVFVTTGRVARSNSGPSVFISYIIAGISALLSSLCYTEFSVQIPVAGGAFSYLRVTFGEFVGYFAGANILMEYVLSNAAVARSFTEYLCCAFGENDPNSWRIEVDGLVKGYNMLDLPAVALIILITICLCNSTKESSMLNLIMTVFHVLFFVFIIVAAFYNGNTENLVKPAGLAPYGAKGVLDGAAIVYFSYIGYDSVSTMAEEIKNPTKSLPIGIVGSVVIVSALYCLMALALCVMVPYDQISESASFSLAFHKMGWKWAGNVVGAGASLGIVASLLVAMLGQARYLCVIGRARLVPLWLAKVHPSTGTPLNATIFLGVCQATIALFTELNIVIEMISIGTLLVFYLVANALIYRRYVISSNNPPQHTLLYLFILSCTSLGFSLSWKLEHQWWGLPLCGGSMIILTAFFQYMVPCDPQSTEWSVPLMPWPAATSIFLNVFLMTTLPKLSYQRFGLWACLVTLLYVFYGVHSTYQGEAEDASISSTNHSNFSTQQEKLDIQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZVR9</accession>
    <name>A0A161ZVR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_020443</name>
        <name evidence="8" type="ORF">DCAR_0625958</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZVR9"/>
    <dbReference type="STRING" id="79200.A0A161ZVR9"/>
    <dbReference type="Gramene" id="KZM92192">
        <property type="protein sequence ID" value="KZM92192"/>
        <property type="gene ID" value="DCAR_020443"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227222"/>
    <dbReference type="OMA" id="SWHTLPR"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000005">
        <property type="entry name" value="DNA repair metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000039">
        <property type="entry name" value="DNA repair metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF31">
        <property type="entry name" value="DNA REPAIR METALLO-BETA-LACTAMASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="227"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM92192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH06530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="583" mass="66669" checksum="5D65FB6CCDB5E83E" modified="2016-07-06" version="1">MPIEMPRGVPFSVDTWTAVSMQKRHHFLTHAHKDHSQGISSFHSYPIYSTHLTKILTLHYYPQLHDSLFVNIEVGDSVVIRDPEADFTVTAFDANHCPGAIMLLFEGTFGTILHTGDCRLDQECLQRLPEKYLGTKTKNPKCSIDYIFLDCTFGRFSKMPSRQSAIRQLINCVWKHPGAPVVYLTCNMLGQEEILVNVSQTFGTKIFVDKEENPECYHLLKLTVPDILTEDSSSRFHMFDGFPNLSERAEAKLADSRNRSQPEPLIIRPSAQWYACEDVHLKTEITRKVKLSEAVRDQSGIWHVCYSMHSSREELEWALQLLRPKWVVSTTPSCRAMELTYVKKHCFISEVASDDPLWKLLDLSFEVPQIAEQVSVKSASCSSILELGAELEPWLIGVSADQQKHLHLSPPSKRPHVTLFGRARFGLPDSEVFHEQKEREIIENDPLNIDEQVAIVGSASNGLQYSSESHEEMRTDMMYGNPSCEVHDEQYKSPEREYTFKETHMKSPENKNVVDIIKTNNSSEVKMTLADETSFSPDGSSKRFCANLRKMYRKMHVPVPQPLPSLVELMKSRKNAKRKFTFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZWE2</accession>
    <name>A0A161ZWE2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_020173</name>
        <name evidence="6" type="ORF">DCAR_0626268</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the APS1/VSP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZWE2"/>
    <dbReference type="Gramene" id="KZM92462">
        <property type="protein sequence ID" value="KZM92462"/>
        <property type="gene ID" value="DCAR_020173"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226465"/>
    <dbReference type="OMA" id="LNPWKTI"/>
    <dbReference type="OrthoDB" id="59415at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07535">
        <property type="entry name" value="HAD_VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010028">
        <property type="entry name" value="Acid_phosphatase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014403">
        <property type="entry name" value="APS1/VSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01675">
        <property type="entry name" value="plant-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF7">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
        <property type="entry name" value="Acid_phosphat_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002674">
        <property type="entry name" value="VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007830757" description="Acid phosphatase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM92462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30050" checksum="A252DBB8F2C89EC7" modified="2016-07-06" version="1">MKAFYTIFLFLSLVYVSSCHENFDPHTLPRPLIVDLYKKLPISEDVALHCTSWRFAGEANNLSPWKTIPQECKDYVDDYINGKAYKSDLERVSREAILYAQTVELKGDGKDIWVFDVDETLLSNLPYYADHGYGLEIFDNVAFDKWVDKGTAEAIESSLKLYEELVKLGFKVFLLTGRSERRRNVTVENLTKSGFHDWEKLILRGAEDQGKPATQFKSEKRSQMVEEGYRIHGNSGDQWSDLSGSAISARSFKLPNPMYYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A2I1</accession>
    <name>A0A162A2I1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_017405</name>
        <name evidence="12" type="ORF">DCAR_0519915</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A2I1"/>
    <dbReference type="STRING" id="79200.A0A162A2I1"/>
    <dbReference type="Gramene" id="KZM94160">
        <property type="protein sequence ID" value="KZM94160"/>
        <property type="gene ID" value="DCAR_017405"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221909"/>
    <dbReference type="OMA" id="HQSCKNK"/>
    <dbReference type="OrthoDB" id="665906at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000006">
        <property type="entry name" value="ethylene-responsive transcription factor ERF014-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051032">
        <property type="entry name" value="AP2/ERF_TF_ERF_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF231">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="32"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM94160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH00549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="231" mass="25709" checksum="E8D4905E60BF17AF" modified="2016-07-06" version="1">MVMKPQVSVESNATASKDIAINTSSVNKKMNKFKGVRMRSWGSWVSEIRAPNQKTRIWLGSYSTAEAAARAYDAALICLKGPTANLNFPTFSQQNFPLNPHQVTAMSPKTIQKLAAQAANASLDNATTSSPEDLPSPPPPSTTSSSSSPSNTIEEDDLSFISKFEDYCKPDQTALDTMDRTAPWFNSFGSFKFRDMIDDMLIFDHPQPSTMNVIEDHFYEEGEDIRLWDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A7M6</accession>
    <name>A0A162A7M6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_016008</name>
        <name evidence="15" type="ORF">DCAR_0414688</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A7M6"/>
    <dbReference type="STRING" id="79200.A0A162A7M6"/>
    <dbReference type="Gramene" id="KZM96630">
        <property type="protein sequence ID" value="KZM96630"/>
        <property type="gene ID" value="DCAR_016008"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219514"/>
    <dbReference type="OMA" id="KHYIRMN"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF60">
        <property type="entry name" value="SHAGGY-RELATED PROTEIN KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="133"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZM96630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG95372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="52330" checksum="BF01B2991A80069D" modified="2016-07-06" version="1">MNVMRRLKSIASGRTSTSDPGGDSRDSSIRRMKIEQDADHKVSDEPLSTEKSSTSAEQYMDSASENTAKPQKLARDELPKQMHQMKIKENKPDNSNKKNIEPTVISGKGTETGQIISTTVVGPNGQQKQTMSYMAERVVGTGSFGVVYQAKCLETGEAVAIKKVLQDKRYKNRELQIMRMLEHPNVVQLKHCFYSTTEKDELYLNLVLEYVAETVYRVSRHYGRINQSVPVLYVQLYTYQMCRALNYIHNVVGVCHRDIKPQNVLVNPHTHQLKLCDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSVGCVLAELLLGRPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPHYTEFKFPHIKTHPWHKVFNKRLPADAVDLVSRLLQYSPKLRSSPLEACAHPFFDDLRQPDACLPNGKALPPLFDFTPQELSGASTELRDRLIPNHARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ABE4</accession>
    <name>A0A162ABE4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nitrate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_014288</name>
        <name evidence="22" type="ORF">DCAR_0416651</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 17">Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrite + NAD(+) + H2O = nitrate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17913"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.7.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="18">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the nitrate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ABE4"/>
    <dbReference type="STRING" id="79200.A0A162ABE4"/>
    <dbReference type="Gramene" id="KZM98350">
        <property type="protein sequence ID" value="KZM98350"/>
        <property type="gene ID" value="DCAR_014288"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216720"/>
    <dbReference type="OMA" id="MNNCWYT"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009703">
        <property type="term" value="F:nitrate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050464">
        <property type="term" value="F:nitrate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02112">
        <property type="entry name" value="eukary_NR_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000021">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.650:FF:000001">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.420.10:FF:000003">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000025">
        <property type="entry name" value="Nitrate reductase [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012137">
        <property type="entry name" value="Nitr_rd_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022407">
        <property type="entry name" value="OxRdtase_Mopterin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000233">
        <property type="entry name" value="Nitr_rd_NADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00559">
        <property type="entry name" value="MOLYBDOPTERIN_EUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="14 17" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="503"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="603"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KZM98350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG97311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="859" mass="96229" checksum="A4768B79FE1C003A" modified="2016-07-06" version="1">MAASMVNRQFHRLEHAGVRSFKPPASPEDKNIVTNVEFASSDDEECMTEFKESIDKGKSELKPSVLDLRDEGTADNWIERNASLVRLTGKHPFNCEAPLARLMHHGFITPVPLHYVRNHGAVPKATWADWTVEVCGLVKRPGCFTMEQLVNDFPSKEFPVSLVCAGNRRKEQNMVKQTIGFNWGASAISTSVWRGVPLHHLLKRCGIYSRKNGALNVCFESAEDLPGGGGSKYGTSIKKEFAMDEARDIILAYMQNGELLSPDHGFPVRMIIPGFIGGRMVKWLKRIVVTTKESENYYHYNDNKVLPSHVDAELANSEAWWYKPEYIITELNINSVITTPCHDEILPVNSYTTQRPYILRGYAYSGGGKKVTRVEVTMDGGESWQVSKLEHQEKPNKYGKYWCWCFWSLEVEVLDLLAAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFRVKTNVCKAHKGEIGIVFEHPIQPGNQSGGWMSKEKTLVKRSVSTPFMNKASKVYSMSEVSKHSSAESAWIIVHGHIYDCTRFLGDHPGGADSILINAGTDCTEEFDAIHSNKAKKMLEDYRIGELITTGYTSDSPNNSLHGATKLVALVPREKIPCKLISKTAISHNARVFRFALPLEDQVLGLPVGKHIFLCASINNKLCMRPYTPTSTIDEVGYFELVVKVYSKGVVPKFPDGGLMSQYLDSIPLGTVLDVKGPLGHIEYTGRGNFTVSGKHKFAKKLAMISGGTGITPVYQVMQAILKDPEDETEMYVVYANRSEDDILLKNELDAWAEKYADKVKVWYVVEKANEGWKYSSGFITEAILREHIPAGSEDVLALACGPPPMIQFAVNPNLEKMGYDINDSLLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B527</accession>
    <name>A0A162B527_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_002759</name>
        <name evidence="5" type="ORF">DCAR_0102894</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B527"/>
    <dbReference type="STRING" id="79200.A0A162B527"/>
    <dbReference type="Gramene" id="KZN10103">
        <property type="protein sequence ID" value="KZN10103"/>
        <property type="gene ID" value="DCAR_002759"/>
    </dbReference>
    <dbReference type="OMA" id="TNHENCE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032451">
        <property type="term" value="F:demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044842">
        <property type="entry name" value="ALKBH9B/ALKBH10B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447:SF2">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="280"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN10103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG83717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="505" mass="55444" checksum="1E2D74901B770DB2" modified="2016-07-06" version="1">MSPAAGGGLPGMTSDSFAKDAIISWFRGEFAAANAMIDALCGHLTEIGGDGKEYEAAFAAIHRRRLNWIPILQMQKFYSIADVANELKAVAAAKKERAMEVAAVEESVKEVDGGEEVADVESGRSDESPKSEITDTGSHEVQHMLPNIEICSDHENCEARRAQINMTKGFVNVVRGLKLYDEIFTETELSKLTDYVNELRIAGQNGELSGETFIMYNQAKQQVKGGNKRELIQLGAPIFGPIKEDATNHCPKSNIEPIPATLQGVIDHLVLWHLISENRKPNSCIINFFDEGEFSQPFLKPPHIDQPISTLLLSESEMAFGRTLVCDNDGNYKGSLMLSLKEGSLLVMRGNSADVARHVMCTSPSKRISITFFRVRTQTHESISTAVAPMTGAMTLWQPGVPNSYALSNGARNGYEEMDMIPKWGVLRSPMVMLAPVRPMVLGPKRMSRGGTGVFLPWNASGTRKHTKHLPPRAQKGRFLALPPPVETHKPEVTADSEMISIEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SWL9</accession>
    <name>A0A164SWL9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0727414</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>gibberellin A12 + 2 2-oxoglutarate + 3 O2 + H(+) = gibberellin A9 + 2 succinate + 3 CO2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:60772"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
            <dbReference type="ChEBI" id="CHEBI:73255"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:60773"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SWL9"/>
    <dbReference type="Gramene" id="KZM86761">
        <property type="protein sequence ID" value="KZM86761"/>
        <property type="gene ID" value="DCAR_023895"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194490"/>
    <dbReference type="OMA" id="MLINEAC"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045544">
        <property type="term" value="F:gibberellin 20-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000003">
        <property type="entry name" value="Gibberellin 20 oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH07979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="375" mass="42498" checksum="86BCC7CC4186BC59" modified="2016-07-06" version="1">MPMPSPVSLKSRSSPTNETKKELVFDASLFQHELNIPAQFIWPDHEKPCSDRSPPLVVPPIDLKGYLAGDPSAISNAIMLINEACQKHGFFQVVNHGIDLELINEAHKHISGFFGMPFLEKKRAEKKAGDISGYASSFTNRFSSKLPWKETLSFRYSADQQSANKVEDYFTNAIGEDFRQFGRICQEYCEAMSNLSLVIMELLGMSLGIGPSYLREFFEGNDSIMRLNYYPPCQKPDQTLGTGPHCDPTSLTILHQDDVGGLEVFFDDKWHSILPDKEAFVVNIGDTFMALSNGIYKSCLHRAVVNNCTPRKSLAFFLSPKMDKVVRPPEALVLDSARNFPDFTWSTFLEFTQKHYRSDMKTLDAFAHWLQDRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TT81</accession>
    <name>A0A164TT81_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0728727</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with the C-terminus of GC5, but not with GC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TT81"/>
    <dbReference type="Gramene" id="KZM87921">
        <property type="protein sequence ID" value="KZM87921"/>
        <property type="gene ID" value="DCAR_025022"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193563"/>
    <dbReference type="OMA" id="CNIMFIE"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000229">
        <property type="entry name" value="Probable Ras-related protein Rab-6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH09271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="206" mass="22790" checksum="2993558C1FD40C88" modified="2016-07-06" version="1">MAPVSALAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVIVYDVASRQSFINTVKWIEEVRSERGSDVIIVLVGNKTDLVDKRQVSIEEAEAKAGDQNVMFIETSAKAGFNIKALFRKIAAALPGMETLSSAKQEDMVDVNLKSSVSSSQSSESGGCPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U286</accession>
    <name>A0A164U286_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_025419</name>
        <name evidence="17" type="ORF">DCAR_0729223</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U286"/>
    <dbReference type="STRING" id="79200.A0A164U286"/>
    <dbReference type="Gramene" id="KZM88344">
        <property type="protein sequence ID" value="KZM88344"/>
        <property type="gene ID" value="DCAR_025419"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195269"/>
    <dbReference type="OMA" id="FKCVENE"/>
    <dbReference type="OrthoDB" id="1720310at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000252">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF5">
        <property type="entry name" value="OS04G0586500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853494" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="23"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="507"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZM88344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH09764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="855" mass="96113" checksum="C9A0C87314A3EF11" modified="2016-07-06" version="1">MLGFSLYLLFLLLPLFFAISSAVLPNYIPTDNILLNCGASSKIASSNGRQWDTDSAYISNTSTTSSTQSEPTPSIDQIPYSTARVFPSKFTYKIPVSAAGQKFLRLYFYPTNYSQSLDMTKSFFSVHTANHSLLSNFSVSLNLQKDLPHLVKEYIITVDESRSLEVTFTPNPKSFAFVNGIEVVSIPDKLYSEGIDDSKHVIKFLGDQDGFPIDDSIALENLYRLNVGETGDISSTDDTGMFRSWDRDEPYLLGSLGLTPTLKIPIKYTSDTPPYTAPEIVYYSARTINLESYGSKLTWVFTVDSGFYYLLRLHFCEFLREIIDSNYRVFYIFINNQTAEEQADVIKWTGGRGIMVYKDYVAYVSDNLDGSNSKTDLWLALYPNMEDNPHYKNAILNGLEIFKLSKGDTLAAPNPESLEPALPPETVKSRHVQKRGPLVSICIGGSLGGLILFSLILVGFLLHKRRTPRSQDIDHKSRSTTNNISSFPSVRSKKFSLEEIKLATGNFDDNFIIGRGGFGNVYKGYLENGVCTVAIKRLNPSSRQGFHEFQTEIKMLSNLRHLHLVPLIGYSNDGAEMILVYDYMVHGTLSSHLYGKNNHPLSWKQRLQICIGAAKGLHYLHSGVERMIIHRDVKSTNILLDENMVAKVSDFGLSRVGPRDPTVTHISTIVKGSFGHLDPEYYKRKHITCKSDVYSFGVVLFEVLCARPAIMPELPMEEVNLAYWARNCYREGNLVEIVDKNLIGEIAVESLNKFGEVGYSCLREQGTDRPSMSDVLWNLEFALQLQESWERLDQDVHSNLGDQNVHLLSDARDYIAKIYHKLFSSTSSVTSGARSGRFKSGIGSVFSEIFNMKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VUL1</accession>
    <name>A0A164VUL1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VUL1"/>
    <dbReference type="Gramene" id="KZM90877">
        <property type="protein sequence ID" value="KZM90877"/>
        <property type="gene ID" value="DCAR_021758"/>
    </dbReference>
    <dbReference type="OMA" id="ETTWDAY"/>
    <dbReference type="OrthoDB" id="5314041at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF38">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="665" mass="74179" checksum="B4AA53289D574299" modified="2016-07-06" version="1">MASTDFPLRWEGTGDQWWYASPIDWAAANGHYDLVRQLLRLDANHLIKLTSLRRIHRLESVWDDDEHFDHVSRNRSEVAHKLFCECETNGSKNSLIEAGYGGWLMYTAASAGDMDFVKKLLKIDPLMVFGEGEYGVTDVLYAAARSKNSEIFRLLLDFALLPRLFIGNDKETEHIGEIPSAYRREMINRAIHAAARGGNLVILKDLLECCSDILTYRDNEGSTILHAAAGRGQVEVVKDLITSSDIINSVDNQGNTSLHVAAYRGQLAAVEVLMHASPPSMYSKNNAGETFLHRAVAGFQIPIFRRVDRQIELMKNLARGNNFVIEEIINAKNNDGRTALHLAVIGNIHTDLVELLMTAHSINVNICDNDGMTALDLLKQRPRSASSEILTRQLISAGGIFNCQDHSARKVIASHLRMRSIESSPGTSFEIPDSEILSQMVTDKGSNACGSARLSPELAQPNKHVLISEKQSPKINIKSGSGTDASQRWRRFLHWPKMRKRKSKPEPEPLEKTVDENSVTSSEEVPVSLRSKYSKPSSLPNHKRALSVRSNAPSPTAKKKFASGLVHGVMQTMPHFNATRRTRSCSFSKSPATSSPYYWDKQKGMYVENEIVAEASCSNSVLGDETPNVLHKPAFAHKRPLINQICFRGKPQHREFYDPSIPSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W5M1</accession>
    <name>A0A164W5M1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0624980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W5M1"/>
    <dbReference type="Gramene" id="KZM91315">
        <property type="protein sequence ID" value="KZM91315"/>
        <property type="gene ID" value="DCAR_021320"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227821"/>
    <dbReference type="OMA" id="ARLVLCM"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000792">
        <property type="entry name" value="ABC transporter F family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001124">
        <property type="entry name" value="ABC transporter F family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000355">
        <property type="entry name" value="ribo_prot_ABC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="423"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="732" mass="81200" checksum="F6217F8613AA50D1" modified="2016-07-06" version="1">MGKKKSDESGANAKAKTNSKDAPKDAKKISVSAMLASMDQKPDKTKKASSSSTAGKSKPKAAPKVSSYVDGLDLPPSDEEDDDIASEEEIETSDANKQTVRHQKTEEKPLQISVTEKELKKREKKEMITAQAAEYAKKVALKDDRDAFTVVIGSRAAVLEGQDDADANVKDITIENFSVSARGKELLKNTSVKISHGQRYGLVGPNGMGKSTLLKLLAWRKIPVPKNIDVLLVEQEVVGDDRSAVDAVVSANEELIQIREEVATLMKSSSAAGDDEDADGTASLDAAEKLTELYEKLQIMGSDAAEAQAAKILAGLGFTKEMQGRPTRSFSGGWRMRISLARALFVQPTLLLLDEPTNHLDLRAVLWLEEYLCRWKKTLVVVSHDRDFLNTVCNEIIHLHDLKLHLYRGNFDDFETGYEQRRKEANKKFENHEKQVKAAKRTGSRVAQEKVKNRAKLTAAKEASKNKSKGKVDDDEPLPDAPRKWRDYTVEFHFPEPTELTPPLMQLIEVSFSYPNRDDFRLSNVDVGIDMGTRVAIVGPNGAGKSTLLNLLAGDLVPSEGEMRRSQKLRVGRYSQHFVDLLTMEETPVQYLLRLHPDQEGFSKQEAVRAKLGKYGLPSHNHLSPIAKLSGGQKARVVFTSISMSKPHILLLDEPTNHLDMQSIDALADALDEFTGGVVLVSHDSRLISRVCGDEEKSEIWVVENGTVEKFPGTFDEYKEELQQEIRAEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WQ27</accession>
    <name>A0A164WQ27_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0625818</name>
        <name evidence="7" type="ORF">DCAR_0625871</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    <reference evidence="2" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X8X2"/>
    <dbReference type="Gramene" id="KZM92853">
        <property type="protein sequence ID" value="KZM92853"/>
        <property type="gene ID" value="DCAR_016098"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223969"/>
    <dbReference type="OMA" id="KCNERER"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743:SF5">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="235" mass="26079" checksum="537201847AB83FC7" modified="2016-07-06" version="1">MAANENKSPDFYAVLGLTKDCSDLQLKSAYKKLALRWHPDRCAAPGNSKYVEEAKKKFQSIQEAYSVLSDKNKRFMYDFGVYDSNDDDDNGMGDFLNEMATMMSQNQSNENVVESFEELKTLFEDMFQDDLASINSSSQSGATASCSSSLFDTFGKNPSLPNKRNFEQNSVKPEPENSFNFDTHFQGFSFGVEQEGSLREGGCAPGEFRSRRSGRKQKVSSSHDVSSGNNAGISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XSL1</accession>
    <name>A0A164XSL1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0519194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XSL1"/>
    <dbReference type="Gramene" id="KZM93541">
        <property type="protein sequence ID" value="KZM93541"/>
        <property type="gene ID" value="DCAR_016786"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224110"/>
    <dbReference type="OMA" id="SWCERDG"/>
    <dbReference type="OrthoDB" id="1913441at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000103">
        <property type="entry name" value="F-box/kelch-repeat protein At5g42360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47712">
        <property type="entry name" value="OS09G0555300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47712:SF1">
        <property type="entry name" value="OS09G0555300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="578" mass="65638" checksum="CDF4C522E94FF266" modified="2016-07-06" version="1">MIPESSAREASFRQDLEVPSASKRRVKSISQKLKKKSVTSGEEDDTAGISFRCLTLYGRSGGCKVGADATEDFGDMVGKRRSNASEEGKGYNTICGTEETAVDCFSYGVKDKFWKKTNKKILELKESQRSSGMHVFLPDDILEMCLMRLPLTSLMTARLVCKKWRNMTRTSRFMQLRREGLYQTPWLFVFGVIKDGFCSGEIHAFDVSFNQWHKIDAEVLKGRFMFSVASIQDDVYVIGGCSSLNNFGKMDRSSYKTHKGVVVFSPLTKSWRKAASMKYARSAPILGTYEICSDCSIIKNQHVRQDRRFLRSRVGGVSDVYEDPHRLSVRRQVRSSLDENEVSFFSSMKSNKFVRPKSDHSSAKVRKRFVLIAVGGVGNWDEPLDSGEIYDSASNKWMEIQRLPVDFGIVCSGVVCNGMFYVYSENDKLAGYDIERGFWVGIQMTPFPPRVQEYYPKLISCNHRLFMLSVSWCEGDGQIGRRNKAVRKLWELDLMYLTWTEISVHPDAPLDWNAAFVADRNLIFGVEMFKIFGQVLDFLTVCDVSVAESNWDHISRNHVAHEVDASSCLTKSLAVLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XZ15</accession>
    <name>A0A164XZ15_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0519455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WIP C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XZ15"/>
    <dbReference type="Gramene" id="KZM93755">
        <property type="protein sequence ID" value="KZM93755"/>
        <property type="gene ID" value="DCAR_017000"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222111"/>
    <dbReference type="OMA" id="DNDHESM"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000523">
        <property type="entry name" value="Zinc finger protein WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001230">
        <property type="entry name" value="zinc finger protein WIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043584">
        <property type="entry name" value="WIP1/2/3/4/5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878">
        <property type="entry name" value="ZINC FINGER PROTEIN WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23115">
        <property type="entry name" value="zf-C2H2_STOP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH00098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="370" mass="42158" checksum="7776C0FF2B709E19" modified="2016-07-06" version="1">MTDPYSNLYSHWFRFNPPQPPHQHHYHHHYTYFSNSPPLPPPPTSTINIPNYFVSPPPRPNSPPLREALPLLTSSPIRKTENHFLDKNTTGFDLNTPSYMDSDYKTIYSTPEPKDENEEDEEITVSLQIGLPNPSAADMAAYNMQNILSASCSSSNSNSTMSTPCYSSNNNDSNSTITGMHQQHISKYWIPTPSQILIGPTQFSCPVCYKTFNRYNNMQMHMWGHGSQYRRGPESLKGTQPTGMLKLPCYCCAPGCRNNIDHPRAKPLKDFRTLQTHYKRKHGIKPFMCRKCGKVFAVRGDWRTHEKNCGKLWYCICGSDFKHKRSLKDHIKAFGHGHASYSVGGMEEEEDDDDPASEVEQDDDDQSSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YG62</accession>
    <name>A0A164YG62_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0520306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YG62"/>
    <dbReference type="Gramene" id="KZM94465">
        <property type="protein sequence ID" value="KZM94465"/>
        <property type="gene ID" value="DCAR_017708"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223849"/>
    <dbReference type="OMA" id="VMIGACT"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031425">
        <property type="term" value="P:chloroplast RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001050">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g33760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000231">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF452">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="629" mass="71497" checksum="ECF1099EC781C646" modified="2016-07-06" version="1">MSKFKQYKRSVFKHESLRYFSTTSAAAVLVADSHNHFSTCNAQTLHSPNCQMQPTINPKFFLSKLVDCRNVCHIRQVHAQVTVTGLLENLFVTNKLLYLYVQYKDMNDACEMFDRMPERDPYSWSVMVGGFAKIGDYMNCFGTFREYVRTGECPDNYTLPNVIRACRDMMNVRMGTLIHNVVCKCGLDVDQFVVAALVDMYARCRVLDDARRLFDRMVRKDLVSWTVMIGACVELGDAGEALVLFDRMSEEGVAPDKVTMVTAVNACAKLGVMYKARQMHDYILGKKFSLDVILGTAMIDMYAKCGSVDSAREIFDSMREKNVISWSAMIAAYGYHGEGRKALDLLPVMLSSGIMPNRITFVSLLYACSHSGLIEDGLRLFSLMKEEYYIRPDVKHFTCMVDLLGRAGRLDQAMKLIEEMTAEKDEGLWSSLLGACRIHGHTELAEKAAHSLLELQPQKPGSYVLLSNIYAKAGRWKDVAKIRELMTHRRLKKVPGWTWIEVENQTYQFSIGDRTHPKSKEIYEKLMNLIKELELKGYVPNTDFVLHDVDEELKLDSLYSHSEKLAIAFGLISTAEGSPIRITKNLRVCGDCHTFSKFVSAVTQRVIIVRDANRYHHFKDGACSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XIK5</accession>
    <name>A0A165XIK5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XIK5"/>
    <dbReference type="Gramene" id="KZM98205">
        <property type="protein sequence ID" value="KZM98205"/>
        <property type="gene ID" value="DCAR_014433"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218547"/>
    <dbReference type="OMA" id="PMLPNIV"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12203">
        <property type="entry name" value="GT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000061">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000092">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654">
        <property type="entry name" value="FI21293P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654:SF59">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR DF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="495"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG97156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="553" mass="62108" checksum="1EFF37E48461D23A" modified="2016-07-06" version="1">MLHGASPPPPPPGFAGIGGAPAMEVEEEGGGGGGEESERTSGSSGGNRWPKQETIALLKIRSEMDAEFRDSSLKGPLWQQVSGKLAELGYNRSAKKCREKFENVYKYNKRTKDGRTTKPDGKTYRFFDELQALDTQSSSQPARIVATSSMLLNTTTQTIPTSTVSSTPSPIASTLNMSTNPIPSIRVGDVPSFNMVSLSNSTFSSSSTSSDEQRGERKRKRKSKWKDFFEGLMGEVIKKQEELEKKFLDELEKRERDRMIREEAWRVQEMTRINREHELLVQERSMAAAKDAAVLEFLQKISDQQKNPSSNPITIPQLQQLQLQIQPLVQPSQPPPPPPPIPQQLVIPQPQPQPQPSTPAVAPPQPQSSRGGTDNLQPPAPSRWPKAEIEALILLRKDLDNKYNETSPKGPLWEDISAGMRRMGYNRNAKRCKEKWENINKYYKKVKESNKKRPEDAKTCPYFHQLDALYKEKSKLSHGSASTPMLPNIVQPEQQWPLQQHRQQQQDTEMEDPESENNDNFNEGDEEDGEDDDDTGAGYEIVPNKQPASSQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y386</accession>
    <name>A0A165Y386_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0417137</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Y386"/>
    <dbReference type="Gramene" id="KZM98791">
        <property type="protein sequence ID" value="KZM98791"/>
        <property type="gene ID" value="DCAR_013847"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219439"/>
    <dbReference type="OMA" id="MPQETEN"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010117">
        <property type="term" value="P:photoprotection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905157">
        <property type="term" value="P:positive regulation of photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009641">
        <property type="term" value="P:shade avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000856">
        <property type="entry name" value="B-box zinc finger protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051979">
        <property type="entry name" value="B-box_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832:SF52">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG97796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="294" mass="32674" checksum="76E00860FC24D24F" modified="2016-07-06" version="1">MKIQCDVCNKEEAAVFCPADEAALCDACDHRVHHANKLAGKHQRFSLLQPSSKQFPICDICQEKRAFLFCQQDRAILCKDCDISIHKANEHTKNHSRFLLTGVKLSATSDIYPSPSSSSVTNKGDTVTSFKKSGNLITKSHPITSPLTDSKASKIANSQPIVNGDGPTSSISEYLEMLPGWHVEDFLDSSSAINGFSKTSDNDLLPFWDDDVDKNLKYFSSENTGIWVPQAPPPQQQPHFDHSSNMAFGSQICFKDPKDTTTNIRYSKRWIDDGGFTVPQISPSISSKRSRTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZJZ5</accession>
    <name>A0A165ZJZ5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0310044</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN00130">
        <property type="protein sequence ID" value="KZN00130"/>
        <property type="gene ID" value="DCAR_008884"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210750"/>
    <dbReference type="OMA" id="MEYIVAR"/>
    <dbReference type="OrthoDB" id="1898799at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF136">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="161"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="803"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="906"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1044"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="7">
        <location>
            <begin position="1153"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1326" mass="151269" checksum="052933675332FCE9" modified="2016-07-06" version="1">MVGVADIPVVGVFVEKLSEYTVEALFRGLRYMFCYKALVDQLSSETEKLNIEEANMSREVEKEKMNGKIIKDHVSKWQVKAQEFQESDDEVSPSCSCIPRLPIPDPVTRLYIGRNAVKKAEAVTQLTNSGKEHLAGEIAYLPEVTFMPKPGTTFEEFQSRKDTYQKLWESLVNEDGPLVHGIYGMAGVGKTRMMENIWENAMKEKIFKKVVCVNVGNEDKVKMKLEMKLQNQIAGLLDCKLESQDVGRRASQLENSLKNGGKILLVLDDVWRAIHLDHIIGTPFVDGSRSKGSKILFTSREMDACLANKCEHPVEIKSLWPVEALSLFKNTVGPDTIEEVGDESLVKKVCDECGELPLLICAVGEALKRKPHISWDDAHKQLKNGKFEKIPGVPPKVYAGIKLSIDNLQDDDAKLCLFLCSFFPEDAVIDMKMLIQLATGSLRIPDVESRVLAMVHYLKTSSMLLNSGEDNETKIHDIIRDVARSIAFTDPKYAFLQVTCKSQDLPSNDNYHSRRFLRIDAENDDVHFDEHLVCPDLHTLWLQSNLQIWRGQYANRPQQFSGGFFRIFANLSCLMLQKVKISLEQFSLQPLSNLRTLSLLECDIGNTDGRLFPKSLESLYIYICNLPEPLDVANLKCLRKLEIDNQDPVLVMENVISTLSSLEELHISCGFIYSYQEYHLKPIVMEIIKLTHLTSLHFEFYKDEGVDNTTFEGTDIFSNLDRYNIFVGTNLNRYVVFDQHWRLPLTRSIEFRGDHSKLWEGLIVRAEEVVFHSIHVEVSSICRRACWKKLGMDECANLEPLASENQPATWFSKLTNLEISSCHKMKYLFSSNTARSLVQLQQLSVENCQSIEAIIMNEGPSVGEPIIFYKLKSLKIDSARLASFYAENSVHPPAEYQPFFDGRVAFPCLETLELIDVPDIRVIWGKDCCKDTLSSFCELKYLKVSCCSELEILIPHDVLHRLRNLENIDVSYCNSLRTLFAPSVIGHLKRLKGLSLKGCGKIRDIIEAGEQVITDGILFPELTAVDLHMAHELKSFWGDHGAAANTSRVSVTLPRLSSICLYHLSGLDSFFHNANYEFHMPVIQKVEVIGCGLSTLFTLSVFKNLEKLEMLFIEECELLKGIIKDGDTPETRDKIITLNHVSRVRLLHLPKFENIFCNGTYECYMPALKYLEIVDCKFSVLFTCSVFRKFQQLEKLRVSSCESLEHIVEELGNDEIFEVNNKCITSSQLSSIKLSDMTNLKSFGCTSNYVFNMPRLQNFELFRCPEIVYFNSSKTNTSLVCVTSDWCTEEDFRDLDEYFIRQDHKRRSTIRENEEEEEPREIETES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AFW8</accession>
    <name>A0A166AFW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_009943</name>
        <name evidence="13" type="ORF">DCAR_0311221</name>
        <name evidence="14" type="ORF">DCAR_0311223</name>
        <name evidence="15" type="ORF">DCAR_0311225</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AFW8"/>
    <dbReference type="SMR" id="A0A166AFW8"/>
    <dbReference type="STRING" id="79200.A0A166AFW8"/>
    <dbReference type="Gramene" id="KZN01189">
        <property type="protein sequence ID" value="KZN01189"/>
        <property type="gene ID" value="DCAR_009943"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214037"/>
    <dbReference type="OMA" id="GMGIAQM"/>
    <dbReference type="OrthoDB" id="1690600at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="18"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="98"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN01189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG91965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG91967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="166" mass="16718" checksum="BE47B9F6971985FC" modified="2016-07-06" version="1">MSSSSFSGDETAPFFGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGIYGLIIAVIISTGINPKAKSYYLFDGYAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVGIILSSRAGQSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BUM4</accession>
    <name>A0A166BUM4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0313160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BUM4"/>
    <dbReference type="Gramene" id="KZN02896">
        <property type="protein sequence ID" value="KZN02896"/>
        <property type="gene ID" value="DCAR_011652"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213428"/>
    <dbReference type="OMA" id="PWIASNN"/>
    <dbReference type="OrthoDB" id="652551at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000585">
        <property type="entry name" value="Probable inactive receptor kinase At1g48480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF32">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG93872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="608" mass="66785" checksum="730847E0D29318AF" modified="2016-07-06" version="1">MSYFEYLAGAVMTFFLLLNAVKADLAADKAVLLALRTAVRGRTLLWNTTESSPCKWVGVQCRNNRISELRLPGMGLTGNLPDNTIGNLTQLVTLSLRFNALSGSLPLDLWSLTNLRNLYLQSNEFSGPIPSFLFSLKNLVRLSLASNNFSGPISSDFSKLNRLGTLYLEKNQLIGEIPNLDFPGLVQFNVSDNLLVGNIPEKLSSMPESAFVGNALCGRPLKSCNGNGTKNKFRFSVGMIAGIIAGSVVVVLAILVVLFLVCRTRRGIKEEVRVYRSSKETEVAKINKEKNAEDEDNLSSTYPGLNEKVGSASGKKLVYFGKTEKGFDLEDLLRASAEVLGKGTFGTAYKAIFDTGMVVAVKRLKEVAVPEKVYREKIEAVGNMNHENLVPLRAYYYSVGENLLVYDYMPMGSLSALLHGNRGAGRTPLNWKTRTAIALGAARGISYIHSQGSTFSHGNIKSSNILLTTSYQACLSDIGLAQLVGPNTSPNRLDGYRAPEVTDARKVSQKGDVYSFGVLLLELLTGKAPTHTFLNEEGVDLPRWVQSIVKEEWTAEVFDVEILKYEHAEEDMVQLLQLAISCSAQYPDKRPPMTEITQKIEELHVSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C902</accession>
    <name>A0A166C902_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0313830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166C902"/>
    <dbReference type="Gramene" id="KZN03452">
        <property type="protein sequence ID" value="KZN03452"/>
        <property type="gene ID" value="DCAR_012208"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211787"/>
    <dbReference type="OMA" id="HDAYTIS"/>
    <dbReference type="OrthoDB" id="1104395at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG94534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="177" mass="19016" checksum="367A22CC7A71C2A7" modified="2016-07-06" version="1">MATNQIMIALLFIFALHFNVGLSTSQVLKGSVSCLDCRKDYDLSGIKIALKCKQAKKLTTTYTNTNGSFETALPSDTSASTISSNCLATVLGGPEQLYTKQKNIFTTLVKIHGYESSYTISNPLSFYTSCPQSHGKCGATNSGTVESSKTFELPPPGDWGLPPAANYIPFFPIIGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CIL2</accession>
    <name>A0A166CIL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0314392</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CIL2"/>
    <dbReference type="Gramene" id="KZN03942">
        <property type="protein sequence ID" value="KZN03942"/>
        <property type="gene ID" value="DCAR_012698"/>
    </dbReference>
    <dbReference type="OMA" id="AQYENFG"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG95090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="861" mass="96390" checksum="AECBF449F1198585" modified="2016-07-06" version="1">MEGGRNYRFTTTFHSFCALLIFFSLNFRYSHSEKEFAVGVILDTGSWNGRMVNSCIMIAASDFYNINGHYQTRIVTQIIDSKGDPLKALEAVQDLLKKNVGALFLGPEMSLDGTLLALLSEKAKVPMFSLGGSLTSSDEYPYFFQLSHSEALQSKATAALVESFQWKTVNFICEDSEFKSGVLSHLFEYFQEKSIQIGHISALSSMISDDGIRAQLNKLMSMQTKIFVVHMSPSLASRLFLSVKSLGMMSEGYAWILTDKTRNFINSEELMQQMEGALALKPYVPPSIKLQNLTLRWRKEFQDEGPFMNVKELSPSCVWAYDAIWVLAGAVEGAYTKQYPKLAEEHSSESKKILMKELVKTRLKGISGDFQIVNWTVSLLKAFEIVNVIREGERRVGFWEKGVGITSEIMHSSLSSNDLKVIIWPGGSITAPRVAWPRGKKLSVGIPKKNSFTEFISLEYDIHSNTTRALGFCADVFEAALEALQYEVPIEYIPFVDANGESKGTYTDLLYQIYLEKYDAVVGDVTITNNRSHFVDFTIPYTDIGVGTITKVKENKDMWIFTKPVGADLCLITAVFFILTAIVIWFIEKPFNKEFQGSLYQQIGTIFLSTLFLSSRQKLSSNLSRFVMFIWVILVLILTSSYTATLASLLTVQQIGLASRGANVGYQTDSFVERAIASNLNFMDYSLRPYSSAEEYADALSKGSKNGGVDGIIDEMPYIKAFLSKYSPDYAMVDSASTTNGFSFAFRKGSPLVPEISRAIAKLREEGTLDTLEKKWYNKPSSLVNQELPPKPQVLKVDSFGGLFVIGGVSLGLALFARIFYIIRSKLNIYNYMFQTLASGNLAIMLRHMISSSELKETTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DEH8</accession>
    <name>A0A166DEH8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SOSEKI DIX-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_006003</name>
        <name evidence="12" type="ORF">DCAR_0206754</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms long polymer filaments with other SOKs proteins polymers (e.g. SOK1, SOK2, SOK3 and SOK4) crucial for polar localization and biological activity. Binds to ANGUSTIFOLIA (AN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SOSEKI family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DEH8"/>
    <dbReference type="STRING" id="79200.A0A166DEH8"/>
    <dbReference type="Gramene" id="KZN05166">
        <property type="protein sequence ID" value="KZN05166"/>
        <property type="gene ID" value="DCAR_006003"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209052"/>
    <dbReference type="OMA" id="CQAGNEN"/>
    <dbReference type="OrthoDB" id="1280899at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090708">
        <property type="term" value="P:specification of plant organ axis polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010369">
        <property type="entry name" value="SOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048351">
        <property type="entry name" value="SOK_DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021182">
        <property type="entry name" value="SOK_magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083:SF4">
        <property type="entry name" value="PROTEIN SOSEKI 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083">
        <property type="entry name" value="UPSTREAM OF FLC PROTEIN (DUF966)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06136">
        <property type="entry name" value="SOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031043">
        <property type="entry name" value="UCP031043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOSEKI DIX-like" evidence="10">
        <location>
            <begin position="53"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="197"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN05166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG87526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="402" mass="44561" checksum="01FF2004AE7D37C9" modified="2016-07-06" version="1">MELRTSNSRLGRSTSNSVVEVLYRDTSTKSVTTSPERTKVWTQPPSKHHSSKVPVVYYLSRNGQLEHPHFIEVPLSSSSSGLYLRDVIDRLNSLRGKSMATMYSWSSKRSYKNGYVWNDLAENDFIYPTHGNEYILKGSELLEPAKLEESMNSTSKQLEISKSADDSDDSPAITRRRNQSWSSIDLQEYKVIYKAESSGELSSQAANASTQTEERRRRRKARNEITDSGLTREPAESTESTREITELTRDEFSPPGSESSTETLETLMKGDGKMIKSVGGRDDLTANNQSSGRMKASTVLMQLLTCGSISFKNCEDHPGLSLVTSQYKRGLPRGGEGTPVGKSAGENSVGKKNKGVFGSLGRGKVKISEDREYFSGSLVDETNKTEVPALKRSSSYNADRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DY69</accession>
    <name>A0A166DY69_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_006659</name>
        <name evidence="3" type="ORF">DCAR_0207498</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DY69"/>
    <dbReference type="STRING" id="79200.A0A166DY69"/>
    <dbReference type="Gramene" id="KZN05822">
        <property type="protein sequence ID" value="KZN05822"/>
        <property type="gene ID" value="DCAR_006659"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209951"/>
    <dbReference type="OMA" id="CMCMLVY"/>
    <dbReference type="OrthoDB" id="1869454at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016971">
        <property type="entry name" value="UCP031277"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116">
        <property type="entry name" value="PLASMINOGEN ACTIVATOR INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116:SF2">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031277">
        <property type="entry name" value="UCP031277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN05822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="384" mass="43313" checksum="734D5C075ED899D5" modified="2016-07-06" version="1">MPQKKFDADALGVVTICLVAILVLICVICIVYSFYFRSRVHNQGFVQLGYFSGPWIIRIAFIVFAIWWGVGEIIRLNFIRREGRALNANYKWQKTVCKCYIVSNLGMAEPCLFLTLVFLLRASLKETESGTLNQKWNYKTAGYILLFCFPVFILQLMVILIGPKFNKGGSYKRHLPHYFTNTVGATKQADDDTALCTFPLLSTIFLGIFAMMITTYLFLLGRRILYLVINKGLQRRVYTLIISVASFFPLRVLLLGLSVLSKPEHFWFEALAFLAFLSFLCCAGVGVFLLVYFPIADSLALKNLQDLEAMRRISDEYADTISLLANRTTIEEIDAGSSSGTSNKHESALFGTMDNYESMGRFVELSLFSASQHSSPPGSPQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FCF5</accession>
    <name>A0A166FCF5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_008451</name>
        <name evidence="2" type="ORF">DCAR_0209548</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KZN07614"/>
        <property type="gene ID" value="DCAR_008451"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KZN07614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90305.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="103" mass="11793" checksum="82841FAC0FA61142" modified="2016-07-06" version="1">MAYGIEITSDLRWHATLLELTRFLTKEKKMHNSWSAMMTPAASALTSNKDRFSKEFVSFLGFCFADFKKQPTPMQALDHPFLNDTITEDEMITCLVDDGHIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">P-type ATPase A domain-containing protein</fullName>
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        <name evidence="5" type="ORF">DCAR_0100900</name>
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    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG81749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN08397">
        <property type="protein sequence ID" value="KZN08397"/>
        <property type="gene ID" value="DCAR_000943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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    </dbReference>
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    </dbReference>
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            <end position="45"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="61"/>
            <end position="106"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN08397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG81749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="147" mass="16388" checksum="00C092E203DF8163" modified="2016-07-06" version="1">MQVSKTKLEEGKIHVYRPLHYLGLAAVALAIFPIFMKAVVALRYFNLSNINILVLITVIAETGERVNADQVQLNTILSIKPGDVIPIDGVVVQGNCDVDEKTLTGEWSHSLTPLQLMDDAIMMMISWILQMELVVHHVAMLIVQVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GUF6</accession>
    <name>A0A166GUF6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_002015</name>
        <name evidence="2" type="ORF">DCAR_0100266</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN09359">
        <property type="protein sequence ID" value="KZN09359"/>
        <property type="gene ID" value="DCAR_002015"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN09359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="11477" checksum="C55D70FC03D22546" modified="2016-07-06" version="1">MWYLGPDNMGQLKPKYLLVPNPSIRDALCLQEIIEIERARERRMSFLTPFKRANQYLRTHRTVDRTLSLMAIVAIGSAFWISSGPDKKPDKDEAVRTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J3U1</accession>
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        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_004421</name>
        <name evidence="6" type="ORF">DCAR_0104780</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J3U1"/>
    <dbReference type="STRING" id="79200.A0A166J3U1"/>
    <dbReference type="Gramene" id="KZN11765">
        <property type="protein sequence ID" value="KZN11765"/>
        <property type="gene ID" value="DCAR_004421"/>
    </dbReference>
    <dbReference type="OMA" id="NYEREAW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF7">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN11765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG85589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="296" mass="34430" checksum="B0F6A5E88CD937E4" modified="2016-07-06" version="1">MIQDIFHSVSSISRGAIKTINSLPTLVRKLFSSFEDTVLNELSNAPIPEGKIHFLTEYAMIYLTRISLHKELLTHIIVSKPTKSLRNQEDDLFLDASGGTPLELHMIWIIISLKINLERKSELYQDSTLRYVFLTTNVNYIIKTITAYPELLKMIGKEYLSKLSNYVVQAAQDYISSIWHRVLHCLRDDGLHYQIPFYNGISRKSVKNRFKAFNTTFEEVCQTQSSMLVPDIHIHCQLHKQMISNLLPAYESFLQKYGMQIQGERYKERYIKYTSEELKFKMLSITEANLALNSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WNF0</accession>
    <name>A0A169WNF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_005717</name>
        <name evidence="16" type="ORF">DCAR_0206426</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">COPII-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WNF0"/>
    <dbReference type="STRING" id="79200.A0A169WNF0"/>
    <dbReference type="Gramene" id="KZN04880">
        <property type="protein sequence ID" value="KZN04880"/>
        <property type="gene ID" value="DCAR_005717"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206222"/>
    <dbReference type="OMA" id="LSLCIQM"/>
    <dbReference type="OrthoDB" id="3979788at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01468">
        <property type="entry name" value="trunk_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="11">
        <location>
            <begin position="237"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="12">
        <location>
            <begin position="319"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="14">
        <location>
            <begin position="562"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="13">
        <location>
            <begin position="668"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZN04880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG87203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="916" mass="99996" checksum="CABDA90A2CA3156F" modified="2016-07-06" version="1">MANQPKISSAYSVTFAPSNPKSPEVRPETKFTPPLSSPVGPTYPSPPVIVPNQIPLPSVRVPSLNTAIQTNFIPSPYGRTPTPSSVTPPNHIALQLARSPSPTSVSPANGIRDGSLYPYLSTPPGPPRFSSPLQPASLPFRTSTATPQQLPLTSGSSLPTSASPSPRYSNSSVDLQDQVSEASEDLLPFIEVTNVLFSASKVLKHKKLVNRTSLGFGALVSSGRDIPLGPQIIQQDPHRCQNCGAYANFYCTILIGSGQWQCVICRNLNGSEGEYIASSKEELRTLPELLSPFVDYVQTGNKRPEFVPVSDSRTSAPVVLVIDECLDEPHLQHLQGSLHAFVDYLPPATRIGIVLFGRTVSVYDFSEELTASADVLPGRISPSRESLKALIYGTGLYLSPVHASRHVAHLIISSLRPYELNFPEASRDRCLGTAVEVALAVIQGPSAELPRGAIKRSLGNSRIIVCAGGPNTHGPGSVPHSFSHPNYAYMENSALKWMENLGCEAHRQNTVVDILCAGTCPVRVPVLQPLAKASGGVLIVHDDFGEAFGVNLQRASTRSAGSHGLLEVRCSDDILITQVIGPGEEANVDNHESFKNDSSLSIQMLSVEEMQCFALSMETRSDIKSDHVYFQFAIQYTNVYLADISRVITVRLPTVDSVSAYLESIQDEVASVIIAKRTLLRAKNSSTANDMRATIDERVRDIALKFGTQTPSSKLYRFPKELNLLPELLFHLKRGPLLGNIIGHEDERSVLRNLFLNASFDLSIRMVAPRCLMHREGGTFEELPAHDLVMQSDSAVVLDHGTDVFIWLGAELASQAGKCAAALAACRTLTEELTEMRFPAPRILAFKEGSSQARYFVSRLIPAHKDPPYEQEARFPQLRTLSAEQRTKLKSSFIHFDEPSFCEWMRGLKISPPEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFD0</accession>
    <name>A0A175YFD0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="12">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0933967</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 10 11">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="12"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFD0"/>
    <dbReference type="Gramene" id="KZM81881">
        <property type="protein sequence ID" value="KZM81881"/>
        <property type="gene ID" value="DCAR_029494"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202474"/>
    <dbReference type="OMA" id="QHYGEHK"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="10">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH14448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="148" mass="16381" checksum="76FDACAA3B8B06B6" modified="2016-09-07" version="1">MEQTFIMIKPDGVQRGLVGEIISRFEKKGFTLKGLKLITVDQPFAEKHYADLSAKPFFRGLVEYIVSGPVCAMVWEGKNVVTTGRKIIGATNPAESAPGTIRGDFAIDIGRNVIHGSDAVESARKEIALWFPEGIAEWQSSLHSWIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGI8</accession>
    <name>A0A175YGI8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_029786</name>
        <name evidence="5" type="ORF">DCAR_0934526</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. PCMP-E subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGI8"/>
    <dbReference type="STRING" id="79200.A0A175YGI8"/>
    <dbReference type="Gramene" id="KZM82288">
        <property type="protein sequence ID" value="KZM82288"/>
        <property type="gene ID" value="DCAR_029786"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000797">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF436">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM82288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH14996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="393" mass="43515" checksum="908A94FDD73C341E" modified="2016-09-07" version="1">MGTTVTTAIQHQEASWHFFLPLNHNFLRYRAMHARPGFLKSKLSSSHAATVELSFAMPSPIIILTPSSTDQPFPAVLISPFPLLPILSNINVYARDIALLGALNLAETFHSFMEKQKTMGKEVEMALMDMYGKCGAPEKAINMQLLNAMSEVYGITPRSEHFGCMVDILARAGLLEEADIFIRNFPFKADVGVWGPLLGGCRLHNDVLMGQKVAEILIELDLSHSRRYVLLTNIYAADNRWTDAEKVRRGMRISKVQKTPGYSLIEQSQWPAYIFQFLFLLEIFGTSGNIAGFICEAIQGVGGIVELALGYLSALYSIIKKARGLFISDEIQSGFVRTGSHVWGFESHGIVPDIVTMAKDRLDEQPEFLLDPNEISEDGTVVLISVSAGCVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMK8</accession>
    <name>A0A175YMK8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0831886</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YMK8"/>
    <dbReference type="Gramene" id="KZM84964">
        <property type="protein sequence ID" value="KZM84964"/>
        <property type="gene ID" value="DCAR_027614"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197419"/>
    <dbReference type="OMA" id="CTALWVI"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH12383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="385" mass="44485" checksum="407E19CCFDE5A2F0" modified="2016-09-07" version="1">MGKGGRMPVNTDQQKPVDQKLIRRVPHQKPPFTISDIKKAIPPHCFNRSIIRSSSYLVFDLIACFLLYHVATSYIPLIPAPLSYLAWTAYVYVQGCFMFAVWVVAHECGHQAFSDYHWLNDSLGFVLHSLLLVPYFSWKISHRRHHANTNSLDRDENHVPRFKNTIRSYYHHFNNPLGRVFIIAFTLILGWPLYLIVNIAGRSYDRFASHFDPYSPIYSQRERAQIILSDIGVLAAGYGLYRIAAIKGLSWVFLVYGAPLHVANGFLVMITLLHHTHLSLPHYDSSEWDWLRGALATVDRNYGILNIVFHHIADTHVLHHLISSIPHYHSQEASEAIKPVLGDYYHYDDTPFYKAMWREAKECLYVEAEEDGDKASGVYWYKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YNB5</accession>
    <name>A0A175YNB5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pectate lyase</fullName>
            <ecNumber evidence="4 9">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0832168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 9"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YNB5"/>
    <dbReference type="SMR" id="A0A175YNB5"/>
    <dbReference type="Gramene" id="KZM85206">
        <property type="protein sequence ID" value="KZM85206"/>
        <property type="gene ID" value="DCAR_027372"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199856"/>
    <dbReference type="OMA" id="TWILAMI"/>
    <dbReference type="OrthoDB" id="1637350at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000009">
        <property type="entry name" value="Pectate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF130">
        <property type="entry name" value="PECTATE LYASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620733" description="Pectate lyase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH12661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="448" mass="49055" checksum="688B1915F50D6EF0" modified="2016-09-07" version="1">MALSKVIVSLSALLLLLLFVGATAVLNNSDHLSSIKSDEKAELQNSVNSSMAARLEDVDGEGLNEHAVSNPDEVASMVDMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLADCGIGFGRNAIGGRDGRFYVVTDSRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQELIMNSFKTIDGRGVNVHIANGGCITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHVWVDHNSLSNCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGNRYLAPANPFAKEVTKRVETPVGHWKGWNWRSEGDLLLNGAYFTPSGAGATSSYARASSLGAKSSSMVGTITSGAGVLGCRRGHQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQE0</accession>
    <name>A0A175YQE0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_026733</name>
        <name evidence="2" type="ORF">DCAR_0832871</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="173" mass="19173" checksum="09E3A282234469F9" modified="2016-09-07" version="1">MASILASVSFHFPSSMTSVSHSFSSSATAALPCKNGQNSRDSSFCLHNSFLRKERNRAKKLVCNAAEYKFPDPIPEFAQQETEKFKSHLVKKLSKKDMFGDSIDEVIGVCTEIFSTFMHTEYGGPGTLLVEPFIDMADTLNERGLPGGPQAARAAVKWAQDCVDKDWKEWNGN</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG81155.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="209" mass="23192" checksum="301F46B5CBDAA01F" modified="2024-05-29" version="1">MHAFEYPAVDSRFNDLFNQGMYNHTTLLMKKILEVYTGFEDIKEIVDVGGGTGATLARIISKYPLIRGINFDLPHVIKNAAPLAGVEHVGGDMFESVPKGEVIFMKWILHDWSDDHCLKLLKNCCKSLPELGKVIIVESVVPEYSDTSSLARLNNAVDSDMIMLALNPGGKERSLREFEELAKESGFAAVKTICSASVYSVLEFSKKTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6R2</accession>
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        </alternativeName>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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    </reference>
    <comment type="subunit">
        <text evidence="9">Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.</text>
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        <subcellularLocation>
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    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG84139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="586" mass="65072" checksum="D9B9C67CECD3A840" modified="2024-05-29" version="1">MADSQHSARSSTVTVNDGDDEVSGGFLPLASGPQQPYVSELLSFTLDRLHKEPELLRVDAERIEKQMQEVAVSNYRAFISAADALLAVRQEVSSIDKHLTSMIAEIPKLTSGCTEFIDSAEQILEKRKLNQTLLSNHSTLLDLLEIPQLMDTCVRNGNYDEALDLETFVGKLSTMHPKLPVIQALAAEVKHTTQSLLTQLLQKLRSNIQLPECLRIIGYLRRIGVYSEYEMRLQFLRCREAWLTGILDDLDQRNAYEYLKGMVNCHRMHLFDVVNQYRAIFADDTSGSEENHDGGLLFSWATHQITSHLKMLKVMLPKIAEGGSLSNILDQCMYCAMGLGWVGLDFRGLLPPLFEEAVLNLFSKNMGTAVENFQLVLDSHRWVPLPAVGFSANSFGDENQEDVTPPPNLMDHPPLAVFVNGVSAAMNELRPCAPIRLKYIIAQELIKGLRAVSDSLLRYNTTRMLRENESVLFLSLCRSFTEVAFPHCATCFGRCYVGGASLITDSKGLFDGINRLVAASISRDLPKPVNNSENKNTTENGEAPPAENGVIDNGGQTESLGAVEKEEHDTSPQTEEKPVNDDATRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W773</accession>
    <name>A0AAF0W773_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W773"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503:SF2">
        <property type="entry name" value="OS04G0455700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503">
        <property type="entry name" value="OSJNBA0006M15.15 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="184" mass="20866" checksum="4E78C96B3F9E4EC5" modified="2024-05-29" version="1">MARLQDPTAISYQLTIKKARNIELNIMGSLFIRCYMSAGDRKRVRFETKEVSSSNMAWNQSFSFDCFGPEESVSNMLLEGSVMFELRRRCGTSFFGRTGKSQLLGKAEVPWNTVYESSTMDIEKWIVMNPKRRLPEGVKPPAIEVGMRVTGAQKALNHRKSSGGEILEEREIFALDAALEFSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7F6</accession>
    <name>A0AAF0W7F6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103743</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7F6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000033">
        <property type="entry name" value="Stromal processing peptidase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000040">
        <property type="entry name" value="Stromal processing peptidase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF33">
        <property type="entry name" value="STROMAL PROCESSING PEPTIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="6">
        <location>
            <begin position="200"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="7">
        <location>
            <begin position="352"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="7">
        <location>
            <begin position="920"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1248" mass="139499" checksum="0DE5690EE1FE5DA7" modified="2024-05-29" version="1">MQASSVMLGATPMVAPVQVKPLVYVPPTSSVVASRTCQTRRITLSKSRRKPARAQFVSNKNVWKPYSSVLVETSAQKVSSDQLRCFSCLHHGARRKYRVNRSMPTVFVDKSSSFLSRRELDNIFVKQAHIPHAVVGPDEPHAASTTWPDGVLEKQELDLLDPQIGGGEITSLEDFLASELPSHPKLYRGQLNNGLRYLILPNKIPANRFEAHMEVHAGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTRSKDSDGDLLPVVLDALNEIAFHPNFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTIAHIEAVFGQTGIENETVTAPTPSAFGAMANFLVPKLTVGLGGNLSQERSSLSADHSKMRKERHAVRPPVEHNWSLPGIGEDVKPPQIFQHELLQNFSVNMFCKIPVKKVRTFGDLRNVLMKRIFLSALHFRINTRYKSSNPSFTSIELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELARYMDALLKDSEQLAAMIDNVASLDNLDFVMESDALGHTIMDQMQSHESLLAVAGTVTLEEVNSIGAEVLEYISDFGKPSAPIPAAVVACVPKKVHVEGVGESEFRITPAEIISAAKEGLEEPIEPEPELEVPKELISSKQLDEPRLQREPCFISVSQDAGSSKVYDKETGITQRRLSNGIPVNYKITSNEAKGGVMRLIVGGGRAAETLEAKGAVVLGVRTLSEGGRVGNFSREQVELFCVNHLVNCSLESTEEFICMEFRFTLRDDGMRAAFQLLHMVLEHSVWLDEAFDRARQLYLSYYRSIPKSLERSTAHKLMQAMLNGDERFVEPTPQSLQNLNLQCVKDAVMNQFVSDNMEVSIVGDFSEEDIESCILDYLGTISASTGSERAQRYHPIIFHQCPPSLHFQQVFLKDTDERACAYIAGPAPNRWGFSCLGEDLFESLRDVSAYDESLVEVQDVKKDMQKRLHSHPLFFAITLGLLAEIINSRLFTTVRDSLGLTYDVSFELNLFDRLNLGWYVISVTSTPAKVHKAVDACKNVLRGLHSSKVVQRELDRAKRTLLMRHEAETKSNAYWLGLLAHLQASSVPRKDISCIKDLTMLYEAATIGDIYLAYEQLKIDDDSLFSCIGVAGAQAGEEIIDEVEPVEHFHGVIPVGRGSSTMTRPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7G3</accession>
    <name>A0AAF0W7G3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0102232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="668" mass="75376" checksum="078F00D9A3C886B0" modified="2024-05-29" version="1">MKMDGKMSKKARNDDKIGGVDRISSLPDQLLHHVLSFSDARLAVQTSVLSKRWKLIWTTLPVLNFTWSSKNTDFTFSSGIKFIDNIFSRRNNTSDVLKLNLFFFYESPQYRTPLVDKCTNYAISHNAREVNVDVLGADYTVTMKLIGLHAIVLNLKLPFDLYLKELDSCWPLPALTTLCLVRPDYLKPYKLPALYLFDLFALRTLLLDGFELPSSVSLPALRTLILHIVEFPENMSDFCRIFRALGNLRNLTLFFWTTLRCDCVIDCPQLVNLEINYCTSKSGKSIVSSNSGKILVLAPKILNFSSVGIFPIKFGAFDLENVNIQLRDSAVIKTVAPSKTKKIFHKQVTHMLVGLSRTKILALDSNTIEALSALSDFLVQTSSPFFMLEHVKVPHGYEESSMSTYLKWFLLGCCPKATIITILPQFKYYLSFTKASVFGQKIEIIEPASLAAQNVLPQGPLTDPTKVLVGSKKLHTKINDDRATQFDVTVEGLGNDGVSSSRRDSDFGLWQGHEVKSEFARLLHNIMNNYPETFEHFTTSDKKLSTMKLNMLCTSVNVFTKISMTQVDTEMIAEYRDVFAGLQKFGFNVSWLVSRLNYIEKLRNSQPLSELHVIDCNINSAKSKLQDLETLRKKKMEEIQKAFGSMGTNLAVGCIGNDLLRAPEFNGN</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF11">
        <property type="entry name" value="BETA-D-XYLOSIDASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85002.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="48623" checksum="8B606C6E252A0B52" modified="2024-05-29" version="1">MYNLGHAGLTYWSPNINVVRDPRWGRALEMPGEDPFVAGTYASNYVRGLQDIEGTENFTDLNSRPLKVGACCKHLAAYDVDNWLGVDRLHFDARVHEQDMVETFLPPFEMCVRDGDVVSVMCSYNKINGIPACADTKLLKDTVRGEWDLHGYIVSDCDSIEVMIDNQKWLNDKPEDTVSQALKAGLDLDCMGSYPKYMGNAVVQGKAREDLIDKALINLYVVLMRLGLFDGNPSLKSLGLDNVCHKDHIELATEAAREGIVLLKNEKETLPLDPKKYNVLALVGPHANATEAMIGNYKGIPCQYTSPINAFSAYAKVKYAMGCGDILCKNESFISEALDATAEEADATIIFVGLDQTVEAEYKDRVNLNLPSYQPQLIQQVSKVSKGPVILVVMSAGGVDISFAKNDSKIKAILWAGYPGERGGQAIADVIFGKYNPGDKHL</sequence>
</entry>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="entry name" value="NUCLEASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        </location>
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        </source>
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        </source>
    </evidence>
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</entry>
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        <lineage>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
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    </comment>
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    <dbReference type="PANTHER" id="PTHR12542">
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WI82_DAUCS</name>
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        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DCAR_0209397</name>
    </gene>
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        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="3 10"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WI82"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018238">
        <property type="entry name" value="Glyco_hydro_14_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF1">
        <property type="entry name" value="BETA-AMYLASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00506">
        <property type="entry name" value="BETA_AMYLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="443"/>
            <end position="444"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="533" mass="59387" checksum="567143071CEB6FE9" modified="2024-05-29" version="1">MALSSSTSFMNLKDIRNTKHSDESSSGMVCFAQMKPSCSLRARSSMPDPSQHFPENSAIEFGRDQQAEKFHGSSRPHSKTESRVPVFVMLPLDTVCSGGRLSKPKAMNASLKALKMAGAEGVMVDAWWGLVEKDGPSQYNWEGYAELVNLVQRNGLKIQVVMSFHQCGGNVGDSCSIPLPPWVLEEMSKNPDLVYTDISGRRNPEYISLGCDSLPVLRGRTPIQVYSDYMRSFQEIQVGLGPCGELRYPAYPESNGTWNFPGIGEFQCYDKYMRASLEASAEAAGNKDWGKGGPHDAGHYNQFPEDTDFFRTDGTWNSKYGEFFLKWYSGKLIEHGDRILSAARGVFQGTGATLSAKIAGIHWHYTTRSHAAELTAGYYNTRNHDGYLPLARMLSNHGVVLNFTCMEMRDREQPEHANCSPEGLVRQVKMATKSAGTELAGENALERYDGGAFAQVLATSRSDSGNALSAFTYLRLNKNLFEAENWRHLVEFVKSMQEGGRDMRVPEIDTRKTDLYVRFISEKNVRSKEAAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJB6</accession>
    <name>A0AAF0WJB6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0208391</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable ATP-dependent zinc metallopeptidase. Involved in the assembly and/or stability of the complexes I and V of the mitochondrial oxidative phosphorylation system.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJB6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000005">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="355"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="774"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG89155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="811" mass="89682" checksum="DB874C2FC67DFB4D" modified="2024-05-29" version="1">MIFSRIGRSLSRSPRSTSLLTNGRAALVGESILQGPGSVDGKLGLVRKYLAANGNLAVSKAYLSDFRYLLANPRLHRYFSTEEGPKKKNYENFYPKDKKEIPKGDEQKSQSKEDSNTDDGGDFQSAFMKQIQNLIIPLLVIGMFFSSFPFGGREEKQISFQEFKNKLLEPGLVDHIVVTNKTVAKVYVRSTPLNRSRGDGPVAQGPDTDNDTRGKKGQYKYYFNIGSIESFEEKLEEAQEALGIDPHDHVPVTYNSEIGWYQEVMRFAPTLLLLGTLMYMGRKMQGGLGVGGGGGKGARGIFNIGKAHITKVDKNAKNKVYFKDVAGCDEAKQEIMEFVHFLKNPKKYEELGAKIPKGALLVGPPGTGKTLLAKATAGESAVPFLSISGSDFMEMFVGVGPSRVRNLFQEARQCAPSIIFIDEIDAIGRARGRGGFSGSNDERESTLNQLLVEMDGFGTTSGVVVLAGTNRPDILDKALLRPGRFDRQISIDKPDIKGRDQIFQIYLKKLKLDNEPSYFSQRLAALTPGFAGADIANVVNEAALIAARSDQTTVTMEHFEGAIDRVIGGLEKKNKVISKLERRTVAYHEAGHAIVGWFLEHTEPLLKVTIVPRGTAALGFAQYVPNENLLMTKEQLFDMTCMTLGGRASEQVMLGKISTGAQNDLEKVTKMTYAQVAVYGFSEKVGLLSFPQREDGGFEMTKPYSNKTGDIIDTEVREWVAKAYEKTIELVEKHKEHVAQIAELLLEKEVLHQEDLLRVLGERPYKPAEMTNYDKFKQGFMEEDDKSKPATESVSEEDDDSAPLVPDVVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJU8</accession>
    <name>A0AAF0WJU8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0309925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJU8"/>
    <dbReference type="KEGG" id="dcr:108215306"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000008">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="36"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="632"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="972" mass="109650" checksum="645A5FB133BAE3B0" modified="2024-05-29" version="1">MEVSDSLINKIYNSFSADVHFAAPISSLSTNEVELVRGVLQILQGFSSSLFYWDKNKQSYRVKRGIFLSHLSRSSLNAILGQFVYSATCLRLVELLVDKVETSIQSPMPTLSAFTSCVTAWLKRLRNIALKEEIGISKTSESGSTPTLLGLASSLSSLCSGAEYLLQIVRGAIPDVYFKLDPRVPAAEISVHVLNHLYKQLNAICLVEAGQEDAYKALLYLFVGSLLPCIQGLDSWLFEGILNDPFEEMFFYANKAIGIDEAEFWEKSYVLRSIKYQKLDDYATEVKADNQDVMGRKPTSVGAYAKGKEQVGRDFQACPLFIKDIGKEIISAGKSLQLIRHAPKTHSVNSGSNEAISQSKSQFTLSEVFCVSLAALIGQGDHISEDMWQDDTVVSLFQSSVETHKSEETGKSMSGVTCLKKLLVDTSPWKRENGFRNMHNIAGDSTDWEREDTSTSSIVDDLLLRRPYCHENPAVTVCHRSLHENKDYWNTLNLSRNFALPPLNDESLREAIFGEKKMPFSSSAGTNYAFGFHFGESEYLRKQEEQYILEQLFPFPTLLPSFKEDLHVSEVLPFQRNSTLTSRVLSWIQSAELKATPLPVVIMQECLVVYMKKQVDKIGSHILTKLMHDWRLMDELGLLRTIYLLGSGDLLQHFLTVIFNKLDKGESWDDDFELNMVLQESIRNSADGMLLSAPDALVVSITKDNGANVEEKLSASALVSTPRKTKGNFDIDGLNSLKFTYKVPWPLELIANTEALKKYNQVMSFLLKVKRAKFVLDKARRWMWKDRSSTTLTRKRHWLVEQKLLHFVDAFHQYVMDRVYHSAWRQLCEGVSAAGSLDEVIEVHEAYLLSIQRQCFVVPDKLWALIASRINTILGLALDFYSIQQTLSSGGTISTIKARCEMEVDRIEKQFDDCIAFLLRVLSFKLNVGQFPHLADLVTRINYNYFYMSDSGNLVTAPATSKLGKSFPVRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLG7</accession>
    <name>A0AAF0WLG7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0309847</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF385">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="6">
        <location>
            <begin position="59"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="255"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1291" mass="146754" checksum="7A9B8A31DB2E19E7" modified="2024-05-29" version="1">MEHYNHWLVVLIWVLPVLVILTIITIIIRSILTRDRPCVDPPSNPFAAPSSTSSSPPGSWDVFLSFHGGDTRGSFTAHLYKALHDAGVRTFMDEPELRTGEEISAGLFNAIHGSKMCVVVLSENFAYSKWCLKELVEIISCQRTKGLVVVPVFYYVDPSDVRHRRKGFMEGLRSQKNAGSVEMVQKWETALAAVGRIKGHHLQKHANENEADVVQKIMNVALLQASMKVHGEEHLFGVDSVIEEIYKKLQMELNEVRVIGICGMGGIGKTTIAKAFYNNYFNKFVISCFIENVKQKSQGADPLVWLLEQLLVKLLRKNDFKVTDVGSGIRLLKEILCFNKALIVLDDLDQSIPSDLQIKLCNLASPGSRIIFTTRDANLLNQLKEDIPEVDIYTVKKLGQNDSLELFSYHTFRKSMPPEMFRELSAEFVTYAGGLPLALKMLGSSLRGRNHVSFWEAMLIKVQKIPMDQIQEILQLSYDELDETEKAIFLDIVFFFVGNDKDDAVYAFKSCDFYPDLGIPVLEERCLITVDECNRLQMHNLIQDMGRKVARKEFNQGNGRYLRLYQENACERLQNLEGADHIELLLLDLTRSKARQLTTKIFRKLCKLRLLEIIDPHNILSGDLKNSCPELRYMRWSHSPWTSVNAKFRSQRLVCLDMSYNRLDSSWKPPASLKSLNMSYSKLKSIPNLSELKVLERLLLQGCQNLSKVPSTISQLSKLGHLDMGSCRLLRELPESIGQLTLLRLFNLSHCVNLNQLPETITQLTRLNHLKLECCYNLKRLPERIGNMQGLITFHARWSGIEQLPDSFGELINLVHLNLFWCKNLTALPDSICKLMFLKELNLGGSHKLKRLPEELGKMQSLERLYAGCEEINELPDSIGELSSLQRLLLYGCQNLSKVHPTIGQLSNLGHLDMGRCRLLKELPESIGQLTLLRHFNLSHCVNLNQLPGTITQLTRLNHLKLESCHNLKRLPERMGNMQGLKTFVASWSGIEQLPDSFGELIKLVHLNLFWCKNLTALPDSICKLMLLKALDLGGCHELKRLPEELGKMQSLERLHADCEQINELPDSIGQLSSLQVLNLSACRNLRYLPSSIWNLTSLSTLQFPSSYREIINLPERVKSTRLERLDLCCNIRLWLPKIQSVSSLKELSITDESQSFSTETLSLLKLSNLHSLILRNHSGCGPSFYELPLNLQQLWVYDNATLEQLPDLSRLKCLRGLVIRRCISLRSLPPLPPHFQSLKVDGCTKLQDVPDLSMLKDIEELSFVRCSNLKSISLKESSLQVCRCLLLSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLS4</accession>
    <name>A0AAF0WLS4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0311550</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLS4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="11">
        <location>
            <begin position="43"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="12">
        <location>
            <begin position="296"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="441"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG92287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="85312" checksum="C3DD128171F46F17" modified="2024-05-29" version="1">MIYKTFQDSSKAVECKSHDFPSHFHFDKDKYLEEEVFIGFGMEVNKLLQQLVSNTKKQLEVISIVGMAGLGKTTLAKRLYNDPYVVSYFYVQAWATCSQVYHKRDLLLSILRSVTKIADYVCKKNENMLAHDLYCALKGRRYLIVIDDLWSNKAWDDLKRCFPDDNNGSKIMLTTRLKDVALHAQSKGSPLSLRFLTEEESLDLFKQKACITGPFFEYLNLTGKSITRKCRGLPLAVVVIAGILKNNLEMDWWAQVEKSISSYIVSHENQYMNTLALSYNYLPQHLRPCLLTFGAFPEDHEIPVHKLIWIWIGEGFIHQNGTKKILEDIAEDYLTDLMWRSLVVVGKKGSNGAIKTCRIHDLLRDLCLKKAEEDHFPPNIYRYNKHCYSCPHSSTNLSSDCTCLSSKVSQSFSKDLSMICDTSKHIRSLDISSIELFVFPSEVLQLVHLRYLELRFRSGNPPESISCLRELQTLIMSSRMNMVVPNNMWKIINLRHLCIKSGENLVKSSDVEEEPSLLENLQTMSLVSPTRPFQHILARTPNLKKLGLCGQLTTNSGDLQFPDLGLLMHLKALKLLNTIPLCKAGRLSDSIIFPKSLTSLSLSNTYLDWSEAWAFEMIPNLEVLKLKFHAFVGKDWETSLGAFPRLKFLKLDELDIVTWTASRDHFPVLQCLQVHRCSNLLMIPEDFGNICTLEWIEFSECSDDAANSATYMQKEQEKNGNDFLKILHNPGETSSYTNTEYSRSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMW3</accession>
    <name>A0AAF0WMW3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pathogen-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMW3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28043" checksum="048B330A9E302C35" modified="2024-05-29" version="1">MEDENKRVEVARDKYRSFLDDAAGTEWRHGGPPVYDQINLLFEHGRTKDWPKGSLEETVQNAIKSWEMELSHKTRIQDFKSVNPDKFRLIVNGREGLSGEETLRLGSYNALLKSPLPEGLQYYKAEEESFQSSHDAFRSAFPRGFAWEVVGVYSGPPMIAYKFRHWGFFEGPFKGHAPTGEMVQFYGMGLLKVDELLRAEDVEIYYDPADLFAGLLRGPKDPPFPESSSDKSAASATITSIKRCPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN99</accession>
    <name>A0AAF0WN99_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0312363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN99"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
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    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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    </dbReference>
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        <property type="entry name" value="OBL-like"/>
    </dbReference>
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        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
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    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="204"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="469" mass="53666" checksum="0F4A86F54EFE046F" modified="2024-05-29" version="1">MARSCSKEFASNYMLLNSENLGFIDLFYIVLSKDLKNRKFIDCSEGAREESSSRRWIIFASIVAQKLLFFLAKPLARFGSKVEYWLNLISSNGGFVGLVQNFLRGRVVEPNKESANFFSITEYTDKRVDLDKSIKPGDWRYLSALSIMAAKASYENKAYLETIVRDHWKMELLGSFNFWNDYQEKANTQAFLCRETRGDEELIVVSFRGTEVFDAEAWSSDVDLSWYELHCGVGKVHGGFLKALGLQKNLGFPKEIEQVDDRPVAYYFIRNMLRELLAANKNAKFIVTGHSLGGALAILFPAILAFHKEDLLLERLQGIYTFGQPRVGDSKFGEFMEEQLKNNSIRYFRTVYCNDIVPRLPYDDNNFMFKHFGECLYVNSFYKGQIVAEEPNKNYFSLLDAIPQMANAVWELARSFVIPYTRGADYKEGALLRVIRLLGVLVPGISAHLPQNYVNASRLVSSDVLRIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQD1</accession>
    <name>A0AAF0WQD1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0313438</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF5">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="6"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG94145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="6423" checksum="E888B78706C69DF6" modified="2024-05-29" version="1">MEHLLGLLRVRVKRGVNLAIRDTRSSDPYVILRLGKQVVYDNDTFSLDDKMGDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WSP8</accession>
    <name>A0AAF0WSP8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0313986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WSP8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="323"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="458"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="277"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG94689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="547" mass="62364" checksum="086E87547F0F5679" modified="2024-05-29" version="1">MSDDFGLSSPNLIQTEYRPLEVLEKEIYTQAEQASISKINVPEERNLVSIDLGHDINKGKEADMGFGGAHHELPCSLSPEIDFPLKNKPDSNSASLFELLGRYEKRGKNLADEHCNPSSEITTSSRKLSTEEIIRVAAERYIEFPGKDLDCVTTFIHPYGSALSSLSLEETRGADLAHLLLAAADKIWDGKIDSARRLLMRCECKASKSGNPVERLASYFSEALQERIFRETGSRRIKMVSVDKTVLATHDDIPFSKVMQFATTQTILDHVAMETNIHIIDLHIRSGIHWPPMIQALSERKAHPIQHLKITALDTEDKQKVEDICKRLESFANPLNIAFSFNVVTVDDIRDLKKELFGIQAGEAVVIYAPTILRTMIPWPDKLETLMRVIGDIRPLLMIVQEVDANDNSPSFINRFVEALFFYSTWFDCLEDCLDRSNQYRMTLENNYFGRGIINCISTEGEERITRSVKIDVWRSYFARFQMVEVEVPKSSLRQAEMVLKKEFPCARFCTLNNDGKCLIIRWKGTPMFSLSTWKFQEAPLYVELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTM9</accession>
    <name>A0AAF0WTM9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTM9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF191">
        <property type="entry name" value="OS05G0489400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MULE transposase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="139" mass="16067" checksum="0EDC690AE6A62A77" modified="2024-05-29" version="1">MDIQVDFNPNVLRRARRKCMKNIKGIDADQYAKLWDYRMDLLQRNPNSTIELQFESGKPQYLTDSCKCLGLPGVFKRMYKCFGRLKHAFKLACRKVIGLDGTHIKNHYKGQILTTVGVDPNNCMYPLAYAVVETEKKDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTT9</accession>
    <name>A0AAF0WTT9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0313275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTT9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000040">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1392">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG93985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="485" mass="54380" checksum="5B87F06714BCF15A" modified="2024-05-29" version="1">MGKDKVKSPHVLLFPLPLQGPVNCMLKLAELLALNGLQCTFLNTDYIQNCLLKHTHVQSRFDNYPEFRFEAIPDGLPEDHPRTGDKFLDVANGIQEVMKPIFVEMLTSGKLSSKSSSPVSFVIADGFYSFAVDLAKEIGIPLMYFETISPCALWTFFCLPRLIELGEVPFKEEDYDRKVSGVPGTKNFLRGRDLPSFFRISDLNHPIIQFILKEIQEIPRSQGLLLNTSENIDGPIISQISTLCPNIYNIGPLHSLHKSILLSAQKASSHTSSSNSLWEDDKSCMTWLDAQPQKSVIYVSIGSIAVMTVDQLMELWHGIVNSGKRFLWALRPGSIAGKDSKYAIPAELSDATKERGCIVSWVFQEEVLAHPAIGLFLTHSGWNSTLESLVEGVPMICWPYIVDQQVISRFVQEVWKVGIDMKDNCDRILIEKTVREIMEDRKDEFEKSSSVMAKLAGQSVADQGGSSHHNFNRLLKDIRAMSVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WU34</accession>
    <name>A0AAF0WU34_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0414821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WU34"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF108">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR VRN1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="220"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG95499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="32590" checksum="52D4BA89C27DFA7E" modified="2024-05-29" version="1">MSIYFFSQMIPEKFARKYRKYLNVRVCLKASKGEIWPIDLVGYGGELWLQNGWPEFASCYSLRFGHFLIFKYQGNSHFDVFIYDKSGIEIDYPFVSTLTVRDEQDSFNQARSTQVGKRTMIDINELKACKKTRANSACIEPCHIGDCWMRKLQHQKIEKVTSDKDLEVKLDDNAQPLGMIDQTKLELNLYNPMHQGDIKRAFTQRYMRSNQNFQVHLHANGKTWPIWCYAFCNRCRFARGWSQFAEDNSLSVGDVCVFELVNRSQNLLQVFIYRATKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUA5</accession>
    <name>A0AAF0WUA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UMP-CMP kinase</fullName>
            <ecNumber evidence="9">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxycytidylate kinase</fullName>
            <shortName evidence="9">CK</shortName>
            <shortName evidence="9">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Uridine monophosphate/cytidine monophosphate kinase</fullName>
            <shortName evidence="9">UMP/CMP kinase</shortName>
            <shortName evidence="9">UMP/CMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0414929</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="9"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUA5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009123">
        <property type="term" value="P:nucleoside monophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>CMP</name>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG95604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="206" mass="22617" checksum="19BE5A3E9BCA47CC" modified="2024-05-29" version="1">MGSVVDSSNKANGSALANKKVTVIFVLGGPGSGKGTQCANIVQHFGYTHLSAGDLLRAEMKSGSENGEMIQNMIKEGKIVPSEVTIKLLEKAMLDNGNDKFLIDGFPRNEENRAAFEAVTGIEPEFVLFFDCSEEEMQKRLLGRNQGREDDNIETIKKRFKVYMESSLPVIEYYNAKGKVRKIDAAKPVEEVFEAVKACFTPVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUH6</accession>
    <name>A0AAF0WUH6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R3H-associated N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0314120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
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                <person name="Grzebelus E."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </evidence>
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    <protein>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Grzebelus E."/>
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                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family. Cyclin T subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X676"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20587">
        <property type="entry name" value="CYCLIN_AcCycT_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20588">
        <property type="entry name" value="CYCLIN_AcCycT_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000026">
        <property type="entry name" value="Cyclin-T1-5 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000028">
        <property type="entry name" value="Cyclin-T1-5 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="68"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="183"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="493"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="496"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH02546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="603" mass="67899" checksum="95E882879A99AD17" modified="2024-05-29" version="1">MAGVLSGDPLQHGLYEGGSSYSNNQSEDGSGGGGQWYYSRKEIEENSASRKDGIDLKKETYMRRSYCTFLQDLGMRLKVPQVTIATAIIFCHRFFLRQSHARNDRRTIATVCMFLAGKVEETPRPLKDVILVSYEIIHRKDPDGAQKIKQKEVYEQQKELILMGERVVLATLGFDLNVHHPYKPLVDAIKKFGVAQNALAQVAWNFVNDGLRTSLCLQFKPHHIAAGAIFLAAKFLKVKLPHDAETSWWLEFDVHPLQLEEVSNQMLELYEQNKVPHSQTSEVEGSAVSVPTPRAPTKAPPTTEERLVINSNSQGRGATSKTGLVKSGSSRPAHDQSYLDISDAPPRTTPNGNSDYASTHINGQPDQKGDIMVNDGQFFGSESFPHQGNPEEVQGGSMYDTEGHDGTDLERNDGRSGTRVAKDFRDKWNSRYKDHRDGKIGHSPREAVINIDKNKVKAALEKRKKARGSTTRKTDFTDEDDLIERELEDGIELAVESEKSRSKREQNWSSDEYGHHSRNQDEDGDGEHETKWQSPLKNEFDNVEEGEVPTFDDTGRSYQSSNIRKRKAGSPLDRSVVGKRHEYMTSVSNQHDHDNFQEDRGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X823</accession>
    <name>A0AAF0X823_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial</fullName>
            <ecNumber evidence="3">2.3.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-ketoacyl-ACP synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0522574</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X823"/>
    <dbReference type="KEGG" id="dcr:108220149"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000015">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF297">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="13">
        <location>
            <begin position="34"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH03180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="469" mass="49935" checksum="1F38DB9BB290122B" modified="2024-05-29" version="1">MRMKRCCNLFQLIPSRLHPLRSLSTFDPPPSFSPRRVVVTGVGMVTPLGCGVETTWKRLIKGECGIRAVAPDDLKMSSFDESTKLHTFDQLSSKVAAFVPCGTNPGEFNDQVWLNSKDHRSMSRFIAYALCAADEALKDANWLPSGPEDKEKTGVSIGGGIGSISDILDASQMICEKRLRRLSPFFIPRILINMAAGHVSMQYGFQGPNHAAVTACATGANSIGDAARMIQFGDADVMVAGGTEASMDALSIAGFCRLRALSTKYNSSPQESSRPFDCDRDGFVIGEGSGVLVLEEMEHAKNRGAKIYAEFRGYGMSGDAYHITQPQADGRGAILAMMRALQQSGFGPDQVDYINAHATSTPLGDMVEAKAIKSVFSNRAASGDLALSSTKGAIGHLLGAAGAVEAIFAILAIHHGKAPLNLNLRKPDPIFSDGFMPLTVSTELPIKAAISNSFGFGGTNASLLFASTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8R3</accession>
    <name>A0AAF0X8R3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0521936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8R3"/>
    <dbReference type="KEGG" id="dcr:108223444"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958">
        <property type="entry name" value="CONDITIONAL LOSS-OF-GROWTH 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH02547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1175" mass="130331" checksum="C86F34E9011AF840" modified="2024-05-29" version="1">MATSYSPNRSPGSSRLQLGSMSRLRSSSVKKPPEPLRRAVADSLSLSHPGNPSAVASEAFRILRDYLAAQTTTDLAYSVIIEHTLAERERSPAVVTRCVALLKRYLLRYKPSEETLLQIDRFCVSIIGECNISPTRKLSTWSRSSSKSGASATSSNISPLPVSSFASGALVKSLNYVRSLVAQHVPKRSFEPAALTGVPATSRQLLPSLSSLLSKSFNSHLNPAGAKDSSDLKEGSAASVSNSPIIETVDGIEGLEFIAFDIFKWRWQGHQRLSLLSPDSEHIMNCQEVSKHSFLEVGAAALLVGDMEAKMKGELWRSFGAVDMPYFDKLLQTSLLTTVTNSASARAHMRAITASKRSKTGSLQIWEDSHVSTYRPRPRPLFQYRHYSEQQPLKLNSVEVCEVIAAVCSATPSPTANLMTMTSKLSSSSGKPSMDVAVSVLIKLVIDMYVMDSATAAPLTLSMLEEMLNSPLLDSKSRAFDLILNLGVHAQLLEPLVADDASTIEEEYSHEPYLDSETQLANQGTVKPDYYKTANSSAIDKFESWILGILYEVLLHLVQIEEKEESIWASALSCLLYFVCHRGKIRRSRLEGLDARVIKVLIQVSRRNSWAEIVHCKLICMLTNMLYEVPDGPTTSTLASPRILVEQVDLIGGIEFVYIEFVLANLRDDRRNLYMILLDYVLHQINEACLATGVSEYSDDESQVIATLLTLADAPEALHISVRLGVDGVGDLLRRSVAAALSRYANCDRLNMLLEKVIEKFDTLVRSLSNLDTEFTHLRHISKSHTYLESIEDGVLRNDVCMKAKLAWATLHSLLHSERVPYRQNGYLWLGDLLMAEISDKKDAIWSNVKNLQQKIALAGVNDYSEDLEVPISIWLLCGLLKSKNNLIRWGFLFVLDRLLVRCKFLLDEKKIQHLGNDVSDQLQEKSRLEKASVVIDVMSTALSLVAQINETDRLNILKMCYILFSQLCLKVLPSSSMSRGDTLHDDANPGTLYGEDPMEDTKNKFGSKNDTLTSETASMAALLLRGQAVVPMQLVARVPAALFYWPLIQLASAATDNIALGVSVGSKGRGNIPGATSDIRATLLLLLVGKCTAEPAAFQEVGGDDFFRELLDDTDSRVAYYSSTFLLKRMMTEEPESYQRMLSNLVYRAQQSNNEKLLENPYLQMRGILQLLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9R5</accession>
    <name>A0AAF0X9R5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0522479</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9R5"/>
    <dbReference type="KEGG" id="dcr:108224067"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042116336" description="Subtilisin-like protease" evidence="9">
        <location>
            <begin position="28"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="12">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="145"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="402"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="13">
        <location>
            <begin position="674"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH03087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="83293" checksum="A2616C3351AE8959" modified="2024-05-29" version="1">MGAATATPLCVFLSLILLSLLHAPTFAREKHSYVVYMGAHSHGKSVSSADLDRVTESHYKFLQSFLTRTEEAKDCIFYSYTRHINGFAATLDDEEAAQIARHPNVVSIFPNRGRKLHTTRSWGFMGLEDHGVVRPSSIWKKARFGEDTIIGNLDTGVWPESESFSDEGLGPVPSKWKGICQNDGDPSFHCNRKLVGARYFNKGYAAVVGSLDPVYDTPRDIEGHGSHTLSTAGGSFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPPEGGNECFDADILAAFDVAIDDGVDVLSVSLGGDAVGFFNDSVAIGSFHAVKHGIFVSCSAGNSGPDAGTVANVAPWQFTVGASTMDRQFPSYVTLGNKIRFKGESLSVEVLPRKHFYPVVRSKDAKAADAYAEDAELCKAGSLDSTKVKGKILVCLRGDNARVDKGQQALSAGAVAMILANNEVSGNEIIADPHVLPASHITYTDGLAVYEYIQSTKSPVAYLTRPTTQLGTKPAPFMAAFSSTGPNLVTPEILKPDITAPGVSIIAAYTREVGPTNQRFDKRRVLFNAVSGTSMSCPHISGIAGLLKTLYPTWSPAAIKSAIMTTARTQDNNMHAMTNASHLKASPFSYGAGHVQPNRAMDPGLVYDLTVDDYLVFLCSLNYNQSQITLFSEEPYTCPKHIILMNFNYPSITVPNLKGSITVTRTVKNVGSPGTYKARIVRPPGITVAVKPTSLKFGKIGEEKKFKITMKLKQRSAAKDYVFGKLIWSDGKHFVRSPIVVKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XC09</accession>
    <name>A0AAF0XC09_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0622925</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108227854"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PPP2R3A_B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoprotein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit B''alpha"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000067">
        <property type="entry name" value="serine/threonine protein phosphatase 2A regulatory subunit B''beta-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF0">
        <property type="entry name" value="MIP22305P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH03526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="539" mass="62063" checksum="87164CE756B99E97" modified="2024-05-29" version="1">MDVDFNGDVGSLDAELLQLAEVSSLAIKSNPFVAEKLFEQWLSLPDTVSMVKSLLNNAKAGVPLNVSGTCSSPNAASSNSIPSMFSAGSTPPLSPRSSSGSPRIVKQRAGPSVLGSPLKVLSEPVKEVIPQFYFQDGRPPPNELKERCLFRINQFFYGHMDGLQINEFKPVTKEICKLPSFFSPALFKKIDANSTGIVTRDAFVDYWVNSNMLTKDTATQIYIVLRQPDLNYITQDDFKPVLRELLSTHPGLEFLQSTPEFQERYAETVIYRIFYYMNRCGNGRLTLRELKRGNLIAAMQHADEEEDINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIRYGNHALTYRIVDRIFSQVPRKFTSKVEGKMGYEDFVYFILSEEDKSSDPSLEYWFKCIDLDGNGVLTRNELQFFYEEQLHRMECMAQEPVLFEDILCQIVDMVKPENESYFTLRDLKGGKLSGSVFNILFNLNKFMAFETRDPFLIRQERENPTLTEWDRFAHREYIRLSMEEDAEDASNGSADVWDESLEAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF21</accession>
    <name>A0AAF0XF21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0626299</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF21"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07968">
        <property type="entry name" value="OBF_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000005">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000173">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000072">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="18">
        <location>
            <begin position="328"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="19">
        <location>
            <begin position="649"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="19">
        <location>
            <begin position="800"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="934"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1117"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="943"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="978"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1117"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH06870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1177" mass="133206" checksum="C36B50D20453A303" modified="2024-05-29" version="1">MSSTKFHVMCSLFNWMQKSKSSAKKRSKFRKFIDNFCKPSDYFSAIRLILPSLDRERGSYGLKESVLATCLIDALGISRESSDAIRLINWRKGGAKTGASAGNFSQVAAEVLQLRQGTTSGELTIKELNDMLDRLASSENRAEKTSVLSELIQKTNAQEMKWIVMIILKDLKLGTSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLSDRSQRHKRQDIEVGKPVRPQLALRVSNAAGAWKKLHGKEVVVECKFDGDRIQIHKNGEDIHFFSRNFLDHPEYGHAMSKIIQKNVVTDRCILDGEMLVWDTSTNRFAEFGSNQEIAKAAKEGLENDRQLCYVAFDILYVGDTSVIHQSLKERHELIRKVVKPIKGRLEILVPNGSTNVQRPSGEPCWSRIAYSVDDVERFFKEIIDNRDEGIVLKDLASKWEPSDRSIKWLKLKPEYIRAGSDLDVLIIGGYYGSGRRGGEVAQFLVGLAERPTTNSYPRRFISFCRVGTGLSDEELDAVVTKLKPYFRKYEYPKKAAPSFYQVTNNSKERPDVWIDSPEKSIILSINSDIRTIRSEVFSAPYSLRFPRIDRVRYDKPWHECLDVQSFIELVHSSNGTTQREGNQGVLMDDKPKRTKARNVEKNNVTVVPSHFIQTNVSDIKGETLIFSSMMFYFVNLPPTQSLDSLHRLVAENGGTSSMNLNNTVTHCIAAESKGIKFQAAKLRGDVIHYSWLFDCCSQKKLLPLKPKYFLHLSEQSKKLLEEEIDEYSDSYFLDLDIVDLKQLLNNVDRLEDSKSVDYYKKKYCPKDKWSCFTGCCIYFHLSTQSNPVSKALLELARRRLKLEVSMGGGKVCSSLSQATHLVIQSLLFVDVGIAGPLNSFSTTDVHHICNNKMHVVGSQWLEDSIESSQRLSEDTYSIKLEGLEDLNVGECKSELKIEDERDIDDVGKQNLLHSPGKDEKHRKTEAVAVSYSSDAPSRKRRGRPASTSRKDKKTVKRQPKRLRARVGNKLAKICESESDVDVCLEDTGKEEESKIDLEMHEIDEMAGRREHVNTVGPDTVKYSESSQRGNVIENMDIEGTERSKWVDTLHGTGMEERKSIGSNEPEKLEVMVDPVQSLLLDMIPSLGTQTNAVTESKPQPQTNAVTESKPQPQTNAVTESKPQPSEPNQVPTKKKKVSYKDLAYNLLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XII7</accession>
    <name>A0AAF0XII7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0626465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XII7"/>
    <dbReference type="KEGG" id="dcr:108224866"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH07036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="496" mass="55072" checksum="FFF8C4E45E4DC002" modified="2024-05-29" version="1">MAYRGEDELLSQKLLQEKGRAVVQRNDQANIFPRFWIESRKLWQITAPSIFSRVAAATMNIVTQAFAGHLGEVELASISIANTVIVGFNFGLLLGMASALETLCGQAFGAKRYSMMGVYMQRSWIVLFFCCFLLLPLYVFATPVLKLMGQPDDVAEQSGIVAVWLIPLHFSFAFVFPVQRFLQSQLKANVTAWISLVVFIIHAFISWLFVYQLELGVIGIALTLDVAWWLLVAFQFGYVYWGWCGETWSGFSMEAFSGLWEFVKLSAASGVMLCLENWYYRILILMTGNLEDATLAVDALSICMSINSWEMMIPLAFFAATGVRVANELGAGDGKGAQFATIVAVLHSTIIGLIFAILIMIFRVPLTMIFTSSPDILNATEKLTYLLAFTILLNSVQPVLSGVAVGSGWQSKVAYVNLGCYYLIGIPLGIILGWVFHLGVGGIWGGMIFGGTAVQTIILAIMTIQCNWELEAEKAVNHIEKWSRIRSNDEQQELRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XLJ7</accession>
    <name>A0AAF0XLJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA ligase</fullName>
            <ecNumber evidence="11">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0729740</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="11"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196143"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000220">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000006">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="14">
        <location>
            <begin position="1129"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="509"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="553"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH10273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1401" mass="156883" checksum="CEF21AD935A5CD35" modified="2024-05-29" version="1">MASKTLTLDSSSLFLNSLNSTPSPLTPFPTDFPQSKRIPNSRFIVDGFRHAGDHSVSYFLSHFHSDHYTGLSPNWSKGIIYCSQITARLLVEVLKVPAAFVVSLPMSEIVVVDDCEVRLVDANHCPGAVQFLFKVRVSDGGFERYVHTGDFRYCDDMKLDDALCEFVGADVVYLDTTYCNPKFVFPVQDESVDYIVEAINRIGVENVDKSKSVLFLVATYVIGKERILVEIARRCNRKIHVDRRKMDILGVLGFGEDEVFTEDESESDVHVVGWNVLGETWPYFRPNFVMMNEIMAAKGYSKVVGFVPTGWTYEVKRNKFAVRTKDSFEIHLVPYSEHSNYEELREYVKFLKPKRVIPTVGVDVEKLDSKHALAMQKHFAGLVDEMAVKQDFLKSFHRGARNSSDKPTNVNTAGIEEKDLASDEVQTFENIDSELHDQAPVPQDILLVNDNDIEELVQELHDCLPAWVTRDQMLDLLQSSNKDVVEAVSYFYEHEIEFHEQAAASTSSLCKSQPGSESDSLFPLTPDTKAHDTRAEKSPLSLRFNSLGTKPAIKHDISPAKKKRNIKNKPSKRARTGSSKEATVPTQHTITKFFSKLVCPDPGADKLVNVCAKSDEENIMSQMDCPSPYKEELEQFMQIVDGSPSLRNYASHILDKSKGDISKALDIYYSDRDCSLDENKGKLTESSKSGTVQNHTGCCSSVQAPKETENLLKMDDVSAVIQPLNKAVSLVPEKDKVHISPREQPTDVASCLVSLPPQDYSPIEHACWKGGQPAPYLHLARTFDLVEEEKGKIKATLMLCNMFRSLLALSPEDVLPAVYLCTNKIAPDHENMELNIGGSIIVSALEETCGANKSKIRTMYNNLGDLGDVAQLCRQTQSLLATPAPLSIRGVFQALHKISIQTGNGSTSRKKGLIMNLMRSCREKEMKYLVRTLVRNLRIGAMMRTVLPALAQAVAMSSSPAERSTENTKQQYQALSAVVLEAYNVLPNLDLLIPSLMDHGIKFSSTTLSMVPGTPIKPMLAKITNGIPQVLKLFSCKAFSCEYKYDGQRAQIHKLGDGSVRVFSRNGDETTSRFPDLVNIIKESCSSEASSFVLDAEVVAVDRKNGCKLMSFQELSSRERGGKDSLIAVDNIKVDICVFAFDIMFANGEQLLSIPLRQRQTYLKKLFGNERAGYFEYARGLIVEADDAVMTNDETLTKMNSFLDDALRSSCEGIMVKSLDVEAGYTPSKRSDTWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLVACYNPDLEEFQSVCRVMSGFSDPFYIEMKEFYSEDKILDKKPPYYQTGEKPDMWFNPELVWEIRGAEFSISPVHHAAIGLVHPSRGLSIRFPRFVRSRPDRKPEECSTAMDIAEMFNSQTRKMDVNVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XM47</accession>
    <name>A0AAF0XM47_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0728437</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XM47"/>
    <dbReference type="KEGG" id="dcr:108193818"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051992">
        <property type="entry name" value="OxStress_Response_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172">
        <property type="entry name" value="OS08G0516900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172:SF96">
        <property type="entry name" value="PROTEIN OXIDATIVE STRESS 3 LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="144"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH08986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="27176" checksum="20DB9609CC124330" modified="2024-05-29" version="1">MSIAIESNRMDRSGFDDTMSFKAIYDSSDSGDRRAADDDDSCSSSSIGKNSDVSGGGQSDSGDGDGEVQSSFKGPLDCLDSLEEVLPIKRGISNFYSGKSKSYTSLSDVSSCSSIKDIVKQEDAYTRKRKNLLAHSNYFDRSRNGNLGSSSGISKRLANSSRSSIALSAMVSSSESNNSSECLGSNSSSPTPSLPPLPPNGRRSKSPNNGLMASPPTPERKFSPWRSFSLSDLQHAATATTESALVNNKRSFVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVC8</accession>
    <name>A0AAF0XVC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933558</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVC8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039605">
        <property type="entry name" value="AHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500:SF18">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
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        <location>
            <begin position="95"/>
            <end position="235"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH14042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="25810" checksum="E985CE0DF7DA8D13" modified="2024-05-29" version="1">MEGEKVIHYSAPAAATEGVASVYAEGKKKRGRPKKYGPKKKQRLDVESPQGLFLYVLFFRNQFHAHNTSRTPVGPYGGQELNHCTNPLLVAFCVCANLGSHVVTVDPGEDVLHKLMSISKREAKIICVLSGSGVVSAVTLRQSNPYGDTMNYEGSFDILSLSGFFIPDDNGGMKSIFGNMNVSVAGPDGRVMGGELAGLLTAAAPVQVAITSCLPSYQEPKQFAPTSSCVHATGEGETQGASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y0X8</accession>
    <name>A0AAF0Y0X8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="12">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="13" type="ORF">DCAR_0936082</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
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        </lineage>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOH16527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="23S RRNA METHYLTRANSFERASE"/>
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    </dbReference>
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        <property type="entry name" value="RADICAL SAM SUPERFAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="methyltransferase_(Class_A)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
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    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
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    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="169"/>
            <end position="402"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="435" mass="48274" checksum="A94F931439ED4DB7" modified="2024-05-29" version="1">MVLIRINYNLLRMASISIHQSPIHLFSNSTHFLHRLSISSSSAATCLSASSSSFSTVCSPDKRISRYENTKAVLKGMTYTEFEKWVSSHGYRPGQALMLWKCLYGDNIWAHSVEDLEGLNKDFKKMLSKQAEFTVLKMKDILTATDGTRKILFTLEDGLVIETVVIPNEKGRTTVCVSSQVGCAMNCQFCFTGRMGLKRNLSAAEIVEQAVFARRLLTSEVGSIANVVFMGMGEPLQNIDNVIKAVDIMVHDQGLHISPRKITVSTSGLVPQLKRFLRESNCALAVSLNATTDVARNWIMPINRKYNLSLLLETLREELKSKHKYKVLFEYVLLAGVNDSIEDAKRLIDLVQGIPCKINLITFNPHSGSKFKPTSDEKMIEFRNILAEGGCVVLLRPSRGDDQMAACGQLGNPGEIQAPLLRVPAQFQAALEAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AFU0</accession>
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        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein bL12 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0100719</name>
    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF1">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L7_L12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="3">
        <location>
            <begin position="49"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG81568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="86" mass="9167" checksum="9241D7E3B03E7A79" modified="2024-05-29" version="1">MKEPPVVGMMKAGSVLGGMGAVKGALGAKEDAMLEKTAFELMLDAPGKIKELVEKIPAVFKKSFSKEEAEQIIMKMKALGAKVVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARF1</accession>
    <name>A0AAF1ARF1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0311944</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARF1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01989">
        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF65">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="422"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="13">
        <location>
            <begin position="703"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG92669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="784" mass="88214" checksum="32EB9514E03B31C1" modified="2024-05-29" version="1">MSFTTSTSTSPAEMTSVAVAVNGHPGGKGGKDSLRAVKWAVENLMSKAHHFVLIHVVPTITSIPSPSGPPIPIKELDANVVTMYLHEMKVKFEDIFVPYKKLCKTREMETLVLEGENPAAVLVRYISDSKITYLVLGSWTSNFMTRTIKGPGIPSTVLKDAPGTCTIYVVSRDNLITNSVNALSTSGRSSKIWLFSQNKHGSFRIKKLPSGHYSSSVESKDFDMIGASSMTAYNDPHSQSLPHGSSHNYSLVVQQGNYQTAEAGVADAHKLNQFTYISSQNVEQYFCEHGMQAEAERVRTELRNTVAMYNQACENLVHAQAKIHLISSDCLEESKRVNAALKREETFRKIAAEEKEKHLEAVKEIEIARNLLTKEGYERELAELNYLRESVEKQKIAHALFLSDKRYKRYTREEIKMATGNFSVNNLIGEGAYGKVYTCKLDHIPVAIKVLRPGADKKHEFLKEVEVLSKLRHPHMVLLLGACPEDGCLVYEFMEKGNLEDHIFRRSGRPPLPWFIRFRIAFEIACGLAFLHNSKPDPIIHCDLKPGNILLDKNFVSKIGDVGLAKLISDVVPDNITEYGDSIIAGTLFYMDPEYQRTGTIRPKSDVYAFGIITLQLLTGCRPKGLILRVEDALENGSIYEVLDKSVTDWPLPEAEELARIALQCSSLRCRDRPDLDQEVLPVLKKLVDFSDSHTEVQRFNIDAPAHYYCPILQEVMVDPYIAPDGFTYEHKAIKLWLEKHNVSPVTKLRLQQKMIHPNHTLRSAIQEWTSRVQHPQVPALKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATP9</accession>
    <name>A0AAF1ATP9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATP9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48137">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48137:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG91995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="38" mass="4571" checksum="DA488A00B7E431A5" modified="2024-05-29" version="1">MSPVISEILCIGFMINTYLRHLVQSFSVCFLYWFYDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU78</accession>
    <name>A0AAF1AU78_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0414447</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AU78"/>
    <dbReference type="KEGG" id="dcr:108203997"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="4">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG95144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="161" mass="19143" checksum="477ACFA2845B7AEB" modified="2024-05-29" version="1">MRPEDNEPPYIARVLSRIHNQIRIAYYYRPKDTVHKRRLFFGKKELFESNNLDVQSAETVIGKCIVHSFDDYTKLDPVHDEDYFSPFKYDYTNKTYTPHPVQLYCKCELPYNPDEWMLHCDKCKDRSKTLGACRYHPACVKMTTVQAEKLKNYLCDDCVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVB1</accession>
    <name>A0AAF1AVB1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0313143</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">(Microbial infection) Interacts with Grapevine fanleaf virus (GFLV) 2B-MP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVB1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000001">
        <property type="entry name" value="cysteine-rich repeat secretory protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000020">
        <property type="entry name" value="Cysteine-rich repeat secretory protein 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051378">
        <property type="entry name" value="Cell2Cell_Antifungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080">
        <property type="entry name" value="ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080:SF3">
        <property type="entry name" value="PLASMODESMATA-LOCATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041908828" description="Gnk2-homologous domain-containing protein" evidence="16">
        <location>
            <begin position="23"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="130"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG93856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="29497" checksum="13C621CDA2BEDADA" modified="2024-05-29" version="1">MAITLELIILFLVSFFPIPSLSISDAFVYGGCSALKYVPNSPYESNLNSLLTSLVNSATYSAYNKFTITGSSPNDVVYGLFQCRGDLAMPDCATCVARSVSQIGSMCRQTAGAAIQLEGCFVKYDNTSFIGVEDKTVVLHKCAPSIGYDTDLMNRRDAVLRSLGGSGGPFRVGGSEDVQGMAQCVEDLSAGECQDCLKEAIGRLKSDCGGAVYGDMFLAKCYARFSTSGAHAYAKSNHGNSHSDGEKTFAIIIGLLAGVALVIIFLTFLKKVFGGHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWE3</accession>
    <name>A0AAF1AWE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0414953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AWE3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37754">
        <property type="entry name" value="CALCIUM ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37754:SF4">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="200" mass="22680" checksum="417FD004E64BA22E" modified="2024-05-29" version="1">MGQVLDKVQGTPFISKSHFFVFCVFEFTWGNYVFQLLHKVVVVPSKQWRRTQIQKISDKVFDRVKKETGHDGLTFEDLYIAVLLVYNEINKRLPGPHFDPPSKEQVKALMLECDLNLDGVLNREEFVKFVKHSTKDTFFVVSQGLIVTLAVAPTVALLTKRSTEGIPHVGRIVQKLPTSVYASIITLAVVLFQNSSQSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AXJ1</accession>
    <name>A0AAF1AXJ1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0416073</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AXJ1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STOP1/2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46352">
        <property type="entry name" value="PROTEIN SENSITIVE TO PROTON RHIZOTOXICITY 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
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        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="47606" checksum="063C391E96B1021F" modified="2024-05-29" version="1">MKILGQLDGQMNGICNAIRNCEWDPKVMLTNLSIMEEKIHQLQDLVHLIVGRRTEAVKGSEELAAQQQQLINADLTSIIVQLISTAGSLLPSAKNTFSSGNPLGSQLGQPGGIGYPSGSSITGNNNVNKVEDHSNQIDLIGNNVEEHDQKDEEEVDEGEHLPPGSYEILQLEKEEILAPHTHFCLICGKGFKRDANLRMHMRGHGDEYKTPAALAKPHKEMCSEAPLIKRYSCPYIGCKRNKDHKKFQPLKTILCVKNHYKRTHCDKSYTCSRCNTKKFSVIADLKTHEKHCGRDKWLCSCGTTFSRKDKLFGHITLFQGHTPAIPLDENKGMVDLSDHGEGNEAPSKVAQTDFDFSSNDFQNILDGKGIANDDTRIFSPLDFDTGSLGGFSDFPRPPFEDSASSLSFLISDACNYPRETGRSSISNNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYB5</accession>
    <name>A0AAF1AYB5_DAUCS</name>
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            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
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    </gene>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF108">
        <property type="entry name" value="OS03G0286100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="275" mass="30669" checksum="EDCDCAF715DD4567" modified="2024-05-29" version="1">MSDSGESSDLVEGEFFMEGLRDVDIELDSGEGRNGVVDDFAFGDGDEEDGDGLGICLDSLQLEEVMDVNNNESNGNGDFEWEEVDGRVEDREVLSMFFEDEGNDDASVLQLRESGGVGDAERSRTRVNLEWEVLLNVNNFGVNHGFGNDDADGGYVDHDEYMTAEYETLFAQFGENENAVLGRPPASKNVVENLDLVLIKEEDVENDDALCAVCKDGMNVGEKAKQLPCSHRYHGECIVPWLGIRNTCPVCRYELPTDDPDYERRKRSLRDVQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYN8</accession>
    <name>A0AAF1AYN8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0518952</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYN8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04480">
        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="6">
        <location>
            <begin position="203"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG99599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="374" mass="42782" checksum="6BA31C9D99DAE5B9" modified="2024-05-29" version="1">MEYTMLKDLNDTHDSWRIRKNGELISVNMIFIDEKENLIHATIKKHLVSRFRHLLREGSAYSIKNPKVVQATGDYGRLSKSVQISRHGFQFVMPADTESRSNDTTILTDVVGLASGIGEVDIVGNNWKKRDLHIITNHSVNATITLFAQHSRKLKFIESSNSNKYTLEEKMFFHRMNIKELEYVATVRGKIFEIKNQFGWYYVACKSCGKNFEAEDGDYKFKVHVHIKDHTGTTTLTLFSNVVERLLDISAKKLVNRLASGDSDHPKELNTLIGKEFIYRLRLNHYNFKQGYANYTVSEIYEPRKCLEHEYESKKALEVCSLESSMVDDAEESGANKVEGGVSGNQLKAAVEVRKRRKMVVLDDDDDNDNGDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYW9</accession>
    <name>A0AAF1AYW9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylinositol 4-phosphate 5-kinase</fullName>
            <ecNumber evidence="6">2.7.1.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0416825</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="6"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYW9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17302">
        <property type="entry name" value="PIPKc_AtPIP5K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000015">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017163">
        <property type="entry name" value="PIno-4-P-5_kinase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF140">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037274">
        <property type="entry name" value="PIP5K_plant_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="8">
        <location>
            <begin position="356"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG97485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="753" mass="85878" checksum="BECF50EAD472B900" modified="2024-05-29" version="1">MSEPTTVILERETDKLKPLIVSKSRSQATTRRVTPTFSTGIVTTSSEKRLPNGDLFIGRFSGNVPHGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATFEGEFKSGRMDGFGTFIGSDGDTYRGEWVNDRKHGYGTKGYRNGDYYEGMWRKNLQDGKGKYVWRNGNEYVGEWKNGLINGRGVLIWRNGNRYDGNWENGVPKGSGVFTWPDGGCYVGSWNKESENRMLNGTFYPGGGNGGCNWKEDMLKFNSKLVAPLMLGDKGSDMRKRSSVEGRGSVEGGRGSMEGGRVFPRICIWESDGEAGDITCDIIDNVEATMLYRDGNMLPRDDVGKFRRNPCCFNREVKKPGQMITKGHKHYDLMLNLQLGIRHSVGKHSSIVRDLKPSDFDPKEKFWTRFPSEGSKITPPHPSAEFRWKDYCPVVFRHLRELFQVDPADYMLAICGNDALRELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLIKMLPSYYKHICRYENSLVTKFFGVHCVKPVGGVKTRFVVMGNLFCSEYRIHRRFDLKGSSHGRTTDKPEDEIDETTTLKDLDLNYVFRLQQNWYQDMIKQIDRDCEFLEAERIMDYSLLVGLHFRDDNTGEKMGLSPFLLRTGKKDSLQNEKFMRGCRFLEAELQEMDRVLAGRKPLIRLGANMPARAERVARRSDFDQCNPGSSETDEVVLYFGIIDILQDYDISKKLEHAYKSLQADPTSISAVDPKLYSKRFRDFVGRIFVEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYY1</accession>
    <name>A0AAF1AYY1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0417082</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYY1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000316">
        <property type="entry name" value="serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF128">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STY46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="13">
        <location>
            <begin position="189"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="300"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="586" mass="66077" checksum="B30E9934E76F4DB4" modified="2024-05-29" version="1">MDDTESCSSRAVDSAPTQIRKRKQKFEVYHEVLRRLKESENEEASQPGFDDELWSHFNRLPTRYALDVNVERAEDVLVHKKLLHVAHDPATRIALAVRLVQAPLSEENSVESLHSTLTEKGDPQSSSYTVRQRHVIRPNSYHLLANVHPPPAFGSSPSLELAVEARISLIPDGSSTMSANLKIFRPRHEITISTTDKPKLLSQLTSLLSEIGLDIKEAHAFSTTDGYSLDVFVVDGWAFEESDHLKSVLEKEIPKIEMKSWSTNHTSSAELGNSDDMLMPDQVSIPKDGIDVWEIDSKQLRFEQKIAAGSFGELYKGTLHGQDVAIKCIKAEYISAGVQKEFAQEVYILRKVRHKNVVQFIGACTRSPSLCIVTEFMSGGSVYDFLHKQKGFFKLPAILKVAIDVSKGMNYLHQNNIVHRDLKAANLLMDEHEVVKVADFGVARVQTQSGVMTAETGTYRWMAPEVIEHRPYDHKADIFSFGIVLWELLTGKVPYENLTPLQAAVGVVQKGMRPIIPQQTHPLIVELLEKCWQQDPFLRPEFSEIIDILQHIAKTVGEEEKPVKRRNRVGEYSQLRQGCSVKSEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1T4</accession>
    <name>A0AAF1B1T4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0622821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1T4"/>
    <dbReference type="KEGG" id="dcr:108225123"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF42">
        <property type="entry name" value="GERANIOL 8-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="56478" checksum="ED1BF73C1E3EAA90" modified="2024-05-29" version="1">MEYLVLIASSLFFLILVQYLFGLYARSKLPPGPIGLPLFGNLLQIGPKPHKSLAQLAREYGPLMRIQLGSVTNVVVSSSEVAREIFQTHDAEFSGRSVPDAVAGGLQNHDVAMPWISAGDQWRTLRKALSIYLTNPKKLDKLQELRLRVVKKMVDHVREISRRGEVVDIGKLAFTTALNQMSNTCFSVDVADFGSNQDVNGFHYAVNTVMEVDGKMNFSDYFPWLKKFDPQGIRKDAKAAYGWLDKLCEKFIMQRMSHRESNLPPHGDLLDSFLDFRQDNPVGFDVKQIKVLLMDLFIAGTDTNSSTIEWAMTELMIHSNIMQKLREEISERKREKAHLEEADILELPFLQSVLKETMRLHLVVPLLLPHKSETNVKLNGYTIPKDTRVVINAWAIARDPNSWENPNQFTPERFLNSEVDYKGRYFSFVPFGSGRRICPGVRLAERVMSLMLVSLVGQFDWELPNNMSPEELDLDDTFGVTSQKAIPLVLIPKTLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3H5</accession>
    <name>A0AAF1B3H5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0521237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06859.1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="168" mass="18814" checksum="E8AC875A0E9BDECD" modified="2024-05-29" version="1">MGPCVSKPSTKDDDVDDHFSKSKLGASNITLVTDMDKWEEMLSEADEAGKIVLVNFSADWCNPCRDVLPVFRSLAETYTSIMFLIVDVDELAEFSTSWEIKATPTFFFLKDGQELDKLVGANKEELKRRTDVMAKSLTNSRSLVSNRDQNSSQSRTDDNAKMQNVGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B537</accession>
    <name>A0AAF1B537_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0625928</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
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            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="EMBL" id="CP093348">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF24">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 3, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="86"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH06500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="375" mass="42339" checksum="3DA00FE3C6AA5E5D" modified="2024-05-29" version="1">MEIPAAFFVVPLGILFLFSGLVVNLIQAFFFSTVWPFSKNMFRRINSQVAELLWLEFVWLADWWAGVKVELYTDAETLHLMGKEHSLIVCNHKSDVDWLIGWILAQRSGCLGSSVPVTKKSAKFLPVVGWSIWFSGAIFLERSWLKDEKTLKSGFQRLRDFPSPFWMALFVEGTRFTQAKLLAAQEYASSMGLPSPRNVLIPRTKGFVTAVSQMREFVPAVYDLTFAFPKSSPSPTMLRLLKGQPSVVHVHLKRHLMKDLPETEDAIAQWCKDVFLAKDKLLDKHEASGKFGDGELQSLGRPLKSLVAMTSWICLLIFVSLKFLQWTSLLSSWMGLAFSALGLATVTVLMQFLIQFTQSERSSPANLLIPPKPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5H0</accession>
    <name>A0AAF1B5H0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0726754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5H0"/>
    <dbReference type="KEGG" id="dcr:108195566"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="158" mass="17111" checksum="4E697B67366EEBCF" modified="2024-05-29" version="1">MGAITTNVEVESSVPAQTIYKGFLLDMDNIIPKILPQAIKRVEIISGDGGIGTIKQITLGEVSQFTIVKQRIDEIDREGLKYSYSIIEGDLLMGIIESIASKFTVVPTEGGCIVKNTAVYTPIGDAVIPEENVKEATEQSGMVFKQIEAYLLANPAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B9V4</accession>
    <name>A0AAF1B9V4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aluminum-activated malate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0728673</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aromatic acid exporter (TC 2.A.85) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B9V4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH09217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="58220" checksum="7CD2A335638CFE1C" modified="2024-05-29" version="1">MVNELESVKKYLYVLMLKAKRFPGMAWRSMWKVGKEDPRRVIHSLKVGLSLTLVSLLYLMEPLFKGIGSNAIWAVMTVVVVLEYTAGATLCKGLNRGLGTLAAGSLAFLMEFIATEYGRVFRAIFIGVAVFVIGAAATYLRFLPYIKKNYDYGVVIFLLTFNLITVSSYRVDNVLKIAHDRFYTIAIGCGICLLMSLLVFPNWSGEDLHNSSVSKLEGLAMSIEACVSKYFKDHEIEPDISKDNTEEDPIYLGYKAVVDSKAYDETLALHASWEPRHSRHCYRYPWQQYVKLGAVLRSFGYTVVALHGCLETEIQTPRSVRALFKDPCTRLAGEVSKALRELADSIRNRRQCSPEVLSDHLHEALQDLNAALKSQPRLFIGSKTNQTSNMLALAAATARQTIDKHLSSVKTDSSALNEWKLSKRVSEKTKEAERKALRPTLSKIAITSLEFSEALPFAAFASLLVETVARLDIVIEEVEELGRMASFKEFDPDDEITVTCENPKVEAASTYNNNLPSHAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDM5</accession>
    <name>A0AAF1BDM5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0935087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X265"/>
    <dbReference type="Gramene" id="KZM86423">
        <property type="protein sequence ID" value="KZM86423"/>
        <property type="gene ID" value="DCAR_023557"/>
    </dbReference>
    <dbReference type="OMA" id="ERIWETR"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="11">
        <location>
            <begin position="32"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="12">
        <location>
            <begin position="278"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="448"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH07595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="783" mass="89679" checksum="35185FB08E2C84B6" modified="2016-07-06" version="1">METSNSSFQKVGSQRDARSTTVVGYDEDARILIEKLKGGRKQLQFISVFGMAGLGKTTLVRKVFTEPLIEYHFHVRAWTTVTQEPKKRDLLLGILKSGFDVDNKNESDMELRATLKRRLSGQRYLVVMDDIWDSKDWNDLMLCFPDDNLGSRIVFTSRLADVPFKVHPGCYKYPLRFLSHEESWNLLRYRVFLEDSCPTSLIKIGQEIARNCKGLPLSLVVMAGILASDQTANWWNQVGEDTSSNVSNAPEQHMKTVELSYKHLPNHLKPCFLYFAAFPEDYEIPAWKLILLWMAEGLIKTTQTTEKFPEDEENSLEKVAAGYLNNLISRSLVMVSKTGSNGGIKACIVHDVLRDFCIQRASKANFLQQLPRYQLHVSNTPTKSTVKRVYSGSDSEKNFILELTRYFQLENLFSPYETLLNKSTDSLKVNTILCCNASLSFYADCYDFYALGAFRVLRVLDLSGTGLSNVPHVMVSLLTLRYVALCIKPVDDHRGFVDLSLPNSMNSLIFLETLIVHNRKENGIHVPYETWMLNRLKHLILRGNIVHQDIPDSQMHCLSNLKTVSTQIPLDQCKKILEYTPNLRKLGIRAALYLSSTKLRIPNLDKLQHLENLSLQNDLNPGYDLTDNLLHRELTSPFMFPATLKKLTLVCTFLKWDEMEQIGMLPNLEVLKLQCEAFRGEKWETTDGGFCRLKHLAFKFIKFVQWSAYSEQFPNLQHLKMDECTQLEEIPIGIGDIYTLERIEIRNCNYAVVQSAHKIIEDQLNKGNDFLKISVFQGMQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZUC8</accession>
    <name>A0A161ZUC8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_021093</name>
        <name evidence="3" type="ORF">DCAR_0625240</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZUC8"/>
    <dbReference type="STRING" id="79200.A0A161ZUC8"/>
    <dbReference type="Gramene" id="KZM91542">
        <property type="protein sequence ID" value="KZM91542"/>
        <property type="gene ID" value="DCAR_021093"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226457"/>
    <dbReference type="OMA" id="FLCTPYS"/>
    <dbReference type="OrthoDB" id="7333885at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36343">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36343:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM91542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="118" mass="13044" checksum="C2609F81BECB070A" modified="2016-07-06" version="1">MATKLAISSSITPILPFSTKLSPPLALPHTVASPTTKAKQFRIYAEPGGGEQADLKQKDKKFITKEQEPEQYWQTAGEREGENPMSTPLPYIILFGMSTPFIILAIAFLNGWIKAPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZZW8</accession>
    <name>A0A161ZZW8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZZW8"/>
    <dbReference type="Gramene" id="KZM80146">
        <property type="protein sequence ID" value="KZM80146"/>
        <property type="gene ID" value="DCAR_000175"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN08296">
        <property type="protein sequence ID" value="KZN08296"/>
        <property type="gene ID" value="DCAR_000842"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36381:SF1">
        <property type="entry name" value="ETHYLENE-REGULATED TRANSCRIPT 2 (ERT2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="36226" checksum="09136BE85D2A59A9" modified="2016-07-06" version="1">MSVQLKKGSSLVDLYDINRGLLKKPPLKKRTKHHNSCGAPKCSTKLVNPERGSKRFLKWEMGKFFVAVLKILFVSAVALMTKRMVLGATVSAFCLFFLEYGGRFLYRLMRGLLICRTKKTSVSFTRTEWDSSEGGFVSGCSSLTVSSEEVCDIPADLDMCFSDFTNSDSNKKLEFEDRGSGEGILVSDQNCNCDGLGTKSRGSRRERVKSKMKKLVPKKIKRSMKKRSAVKRGGTDVLEDDNCEAKEYDTEVDASDDSEDSDVEAKAEKARDVRRFISKYWFLCFIALAGLFQGRSLAVVLTLFWCLIVKLVECTIKHRKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_010047"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="109"/>
        </location>
    </feature>
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        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN01293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="131" mass="14898" checksum="49B4C8F711626E3F" modified="2016-07-06" version="1">MQNKRKKCATQETTKSADIEKGAGNEDFVMEKIDHPDEKERERLENFRANLIGIGLGKLFMPTPGFKNMDHQHNGTPAADTPPVQEDFDADEENESDEDQPLSIRRSTRLSMKTKFKFTNTPETTINLDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U6H0</accession>
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    <gene>
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    <organism evidence="8 9">
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <reaction evidence="5">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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        </reaction>
    </comment>
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            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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        </reaction>
    </comment>
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    <dbReference type="EMBL" id="CP093349">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0016491">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH09941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="120" mass="13949" checksum="3B281F4B6B724928" modified="2016-07-06" version="1">MPRRDDARDLLFNSDRDFLVKSNSDELVMREQLEPKVVVLQFEHLTPDYNTTWLSTAHLTDIYNELLSDVDRPFEVVFIAVGDGKEDEFRDMFCSMPWTAVPFSDSVARKQLAKKFIASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UIY0</accession>
    <name>A0A164UIY0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_026013</name>
        <name evidence="3" type="ORF">DCAR_0729879</name>
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    <organism evidence="2">
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            <taxon>Apiaceae</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UIY0"/>
    <dbReference type="Gramene" id="KZM88938">
        <property type="protein sequence ID" value="KZM88938"/>
        <property type="gene ID" value="DCAR_026013"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195008"/>
    <dbReference type="OrthoDB" id="1107553at2759"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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        <property type="entry name" value="F-box_dom"/>
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        <property type="entry name" value="SCF_F-box_component"/>
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    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
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    </dbReference>
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        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
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        <property type="entry name" value="F-box-like"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PROSITE" id="PS50181">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM88938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="387" mass="44429" checksum="3EC6229C1BBB824B" modified="2016-07-06" version="1">MATLNDIPEGLLLKIFTAQPVKSLLRLTSVCKLWYQLINSDFFVKSHLAYQKSSLFTNKTLFIRHRGSYSTNLVDGEPSNNVFESHGYDMLPVHSEFNPNVRLRCYGICDGLVCLSHGVGKLRACCPVYLWNPGVRRVLVLPPMVNDPDQRVFNYSYLCFGQHEGDYKVISVVPMGGVYKVYVYSLNQDSWDLLEFYDESKQTSNFDIWPHVWARFVNGAAYFVSGFSRPNRGIVCFDLSRKVIRRMNLPHNDFCVYNFLMEEYQGSIALIENDGDDHQVDVWELRANGNDNSYLWNKKLSMTPETMDTAMGMGTHLGAFGFVNKDKVVLWMTGGTYSGYKYFLLNLENKSVQPFTIPERYFGQLDRHVNVLYDLTESLVLLAKNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UL18</accession>
    <name>A0A164UL18_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0729976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UL18"/>
    <dbReference type="Gramene" id="KZM89021">
        <property type="protein sequence ID" value="KZM89021"/>
        <property type="gene ID" value="DCAR_026096"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193786"/>
    <dbReference type="OMA" id="LNHKNWD"/>
    <dbReference type="OrthoDB" id="1904066at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000549">
        <property type="entry name" value="ARM repeat superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF8">
        <property type="entry name" value="TORTIFOLIA1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH10507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="573" mass="63108" checksum="55D7A8A72BAA2F00" modified="2016-07-06" version="1">MAAKDFKHNVLSCLTRLSDRDTYSAAATELEQIAKALKHDNILPFVACITATDSSDKSLVRKQCVKLISIISQTHGNLISPHLAKILSSVIRRLCDPNSAVRSACIDAITSLASHVTKPPFSLIAKPIVDALVTAQEMNFQSGAALCLAAAINGSPDPEPNFLKRVMQRLEKLLRSESYKPKAALLSVFASVIGVNGALSQQGVRNLVSCLVEFVSSEDWGARKAAAEALEKLAVVERNLMIEFKVSCLKTFEARRFDKVKVVRESMNEMVKAWRELPDVLSEVSPTTKSQCSSNEFASDGQNPQSSKKSIDSTSGALQKKQNHSFVNDSPRTNDPSPVSASKTSLNKAAPAMFRKLDRVMPTNQKVNTATPVTVASVGSNWSEKVFDNPEEKKNKIDVPRARSHPDPVPFKDNVSESSVVARNETEDVYRTEKDGENLSVIQKQLLQIENQQSILLNILQGFIGTSRNGMRALESRVHGLELALDEISYELAAPSGRMSNTNSTPKICFKLPGTGFINSKIRRTISPLLVHNMADENCRSNAFRLKDRRFQQQGDCGFIINPLAEVHDYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UPZ5</accession>
    <name>A0A164UPZ5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0730173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UPZ5"/>
    <dbReference type="Gramene" id="KZM89201">
        <property type="protein sequence ID" value="KZM89201"/>
        <property type="gene ID" value="DCAR_026276"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193303"/>
    <dbReference type="OMA" id="ELYMIQM"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF48">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH10703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="441" mass="51841" checksum="95CB0396718054E7" modified="2016-07-06" version="1">MKSHAIHTNHYNKIPKIIPFLGIALLLFTIIPFYYPFQKPSVVKSTSPETPTLVTVIDHSDVNLVEINEHEDCDLFSGEWVRDPDAPYYTNKTCWAIHEHQNCVKYGRPDLDFMKWRWKPDGCELPGFNPYQFLELVRDKSLAFVGDSIGRNQMQSMICMLSRVEFPVENSYTSDQNFIRWKYMSYNFTLSMYWTPFLVRAEEADPNGPTKTGLFNLYLDEFDLKWTSQIEDFDYVIINAGHWFSRPGFYYEKGQIVGCRFCQVENITDYPMTFGYRKAFRTAFRAINSLKNFKGVAYLKTYSPMHFENGNWNDGGDCVRTKPLKSNEAKLEGINLELYMTQIEEFKIAEQEARRRGKRFRLMDLTPAMLLRPDGHPSRYGHWPNENVTLYNDCVHWCLPGPIDTWSDFLLQMIKTEGRRSYEQRLQLKHKKAGSSKFMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VYY9</accession>
    <name>A0A164VYY9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_021579</name>
        <name evidence="9" type="ORF">DCAR_0624662</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VYY9"/>
    <dbReference type="STRING" id="79200.A0A164VYY9"/>
    <dbReference type="Gramene" id="KZM91056">
        <property type="protein sequence ID" value="KZM91056"/>
        <property type="gene ID" value="DCAR_021579"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM91056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH05248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="218" mass="24138" checksum="1140F7C67685B9CB" modified="2016-07-06" version="1">MENIQESKLTNQGDDDRESIRSGEGDVDSMTTKRTRDQGGRETLIPSKGIGILLSGLFGTGNESSVSIENAGLLALTRVGKFGAVFASIPSPIFAALYCLFFAYVGAAGVSFLQFCNLNSFRTKFILGFSVFLGFSIPQYYWKMHIFWTTHYIRKTVQSERTEANTGGTSSGHTKLTQEVRNSTHYPLDSINIFPQRGSQIVDLEGALLTYLMFFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WM00</accession>
    <name>A0A164WM00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_020671</name>
        <name evidence="8" type="ORF">DCAR_0625684</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WM00"/>
    <dbReference type="STRING" id="79200.A0A164WM00"/>
    <dbReference type="Gramene" id="KZM91964">
        <property type="protein sequence ID" value="KZM91964"/>
        <property type="gene ID" value="DCAR_020671"/>
    </dbReference>
    <dbReference type="OMA" id="VHSGRMK"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF95">
        <property type="entry name" value="EXOSTOSIN GT47 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="2"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM91964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH06261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="37776" checksum="351AF8A20D9AAA6D" modified="2016-07-06" version="1">MNSSLKIFVYPHKPNHPFANVLLPYKFEPGGNYASESYFKKALMTSHFITNDPSEADLFFLPFSIASLRHDKRVGVGGIQDFIRDYIYEISHEYPYWNRSGGADHFYVACHSIGKMAMEKAVEVRGNAIQLVCSSNYFLQGYVPHKDASVPQIWPRQGDPPNQPPSKRKTLAFYAGAMNSRVRESLVGLWKNDSEISVHQNRLKTPYHESLLGSKFCIHAKGFEVNTARIGDALYYGCVPVILADHYDLPFADTLNWKSFSVVVSTADIPELKKILKEIEFEEYVKLQKNVMEVRNHFQWHTFAVNYDAFYMVMYELWLRRTTIRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XK94</accession>
    <name>A0A164XK94_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_016529</name>
        <name evidence="4" type="ORF">DCAR_0518890</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XK94"/>
    <dbReference type="STRING" id="79200.A0A164XK94"/>
    <dbReference type="Gramene" id="KZM93284">
        <property type="protein sequence ID" value="KZM93284"/>
        <property type="gene ID" value="DCAR_016529"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222712"/>
    <dbReference type="OMA" id="MGRDYYK"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000030">
        <property type="entry name" value="DNAJ heat shock family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000020">
        <property type="entry name" value="DnaJ subfamily B member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF524">
        <property type="entry name" value="DNAJ HEAT SHOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM93284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="347" mass="38545" checksum="6DE663E8BA2EFF5B" modified="2016-07-06" version="1">MGVDYYNILKVNRNATVDDLKKAYRRLAMIWHPDKNPTHQRTEAEAKFKNISEAYDVLSDPQKRQIYDLYGEEALKSGQVPPPPPASRVGHPGSYANNYYHQKHPNPSFRFNPRNAEDIYAEMFSGSEGGASGSGGGRSRAYNDGFFRGSETEFGNSSGGSGGAQRKAAAVENVLPCSLEDLYKGAKKKMKISRTIVDASRTRNVEEILTIDIKPGWKKGTKITFPEKGNQELGVIAADLIFVIDEKPHAVYTRDGNDLIMNQEVSLVESLTGKIFEITTLDGRIVPIAVTEIIKPGHELVVRNEGMPISKDPRKKGNLKIKFDVKYPTRLTAEQKSELRRVLGGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YQS4</accession>
    <name>A0A164YQS4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0520719</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YQS4"/>
    <dbReference type="Gramene" id="KZM94857">
        <property type="protein sequence ID" value="KZM94857"/>
        <property type="gene ID" value="DCAR_018099"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223685"/>
    <dbReference type="OMA" id="FLCICIN"/>
    <dbReference type="OrthoDB" id="1430376at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000006">
        <property type="entry name" value="Pectinesterase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF117">
        <property type="entry name" value="PLANT INVERTASE_PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH01337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="202" mass="21757" checksum="55F97EBDF463F5FC" modified="2016-07-06" version="1">MEGFSKNFTSATAFYLLLVFLCICINSSIAINYGAEKASTKFIKTSCSTTNYPSLCFTSLSSHTNAIQTSSRLLAQTALSVALNHVALTSSALVRLSKAPGMKPREVGPMNDCLEELTDSIDELKRSMGEMSQLNGSPNYALLISDIQTWVSAALTDEDTCMDGFDGKAGNTKNVVRGRILNVVHLTSNALSLINNYNSLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YYE6</accession>
    <name>A0A164YYE6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_018296</name>
        <name evidence="5" type="ORF">DCAR_0520924</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YYE6"/>
    <dbReference type="STRING" id="79200.A0A164YYE6"/>
    <dbReference type="Gramene" id="KZM95054">
        <property type="protein sequence ID" value="KZM95054"/>
        <property type="gene ID" value="DCAR_018296"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221625"/>
    <dbReference type="OMA" id="SRCKNTE"/>
    <dbReference type="OrthoDB" id="770224at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="368"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="187"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM95054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH01540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="447" mass="50793" checksum="F3A69CEEAA395611" modified="2016-07-06" version="1">MAYNFSASGINDSQGSYRPLASLEYKEQKLSTADIMRVAGERYIQFSNNHLDAITMFIHPYSSSLSALSTEEASGADLAYHLLTAAENLTYKQYDRASRSLTQSQSMASDTGNPVQRVSYYFAAALRERIDRETGRFTNQEGGPTGFTSNTDLDTFYSSDQELPLSKVTQFAAVQTVIDSVANDVKVHIIDLQIRSGVQWTALMQALAKRNSCPLELLKITATATTDRENVEETGKRLQRYAKFLNIPFSFKIVVIPDMKDIKEELFDIKTGETVVVYSPTVLTTMISRPERVEALMRVIRRFKPSIMVVIEVEANHNSPSFVNRFIEALFFYSAWFDCLEDCMDRNNEYRVILEKNYFSRGIQNIVATEGDERVTRSVKINVWREFFARFRMEETEVSTLSLCDATLALQTQFPCGNSCTLDSNKKCLIVGWKKTPIYSVSAWRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YGH1</accession>
    <name>A0A165YGH1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0417444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YGH1"/>
    <dbReference type="Gramene" id="KZM99061">
        <property type="protein sequence ID" value="KZM99061"/>
        <property type="gene ID" value="DCAR_013577"/>
    </dbReference>
    <dbReference type="OMA" id="CFSNGIM"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33659">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33659:SF11">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG98103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="131" mass="15026" checksum="9E4B078E373FEC12" modified="2016-07-06" version="1">MEGEEADVGRKRMRQSVEDKQIMVTDAEETVGEETEDGVPLSEQMQLDIASIHEKINSFTNLVSELMESGKSLLLEISNQFGERLISIHKEQTDKWEQEIRELRLLDAANEETNSLLHDARYLLQNVHIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DVE9</accession>
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        </alternativeName>
        <alternativeName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
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    </organism>
    <reference evidence="10" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the universal ribosomal protein uL11 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_006568"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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                <person name="Macko-Podgorni A."/>
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    </reference>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="gene ID" value="DCAR_003484"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR058196">
        <property type="entry name" value="zf-C2H2_STOP1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46352">
        <property type="entry name" value="PROTEIN SENSITIVE TO PROTON RHIZOTOXICITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46352:SF14">
        <property type="entry name" value="PROTEIN SENSITIVE TO PROTON RHIZOTOXICITY 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23115">
        <property type="entry name" value="zf-C2H2_STOP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23118">
        <property type="entry name" value="zf-C2H2_STOP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG84510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="346" mass="38554" checksum="27240249B29E5A1D" modified="2016-07-06" version="1">MSSSGDLRHFPANTITSSSLLTADPSIPLLNLAAVQTRIDAVRTILSNSLQTQTLIGKVQLQSISDEITGSIQQIVVNGTALISSSIPPTSPARPDPTLMFLDPELQSRSDIKPDIKLDTVSDDWETIELGSEELLAEFAHFCDVCGKGFKRDANLRMHMRAHGNEFKTLEALAKPEKGLNFGVKKIRFSCPMIGCNRNKCHDKFKALKTVVCVKNHFKRSHCPKSYVCSRCNKKSFSVMSDLRNHLKHCGEVKWKCTCGTSFSRKDKLFGHMALFEGHMPAIMEDEEKGKEVVEEKDSGVNVSENRKFEGLIGEYGSIEEFCLDDMLNTSNDCSLVTQMDGFFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IMK5</accession>
    <name>A0A166IMK5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_003943</name>
        <name evidence="22" type="ORF">DCAR_0104203</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 20">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The LHC complex consists of chlorophyll a-b binding proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IMK5"/>
    <dbReference type="STRING" id="79200.A0A166IMK5"/>
    <dbReference type="Gramene" id="KZN11287">
        <property type="protein sequence ID" value="KZN11287"/>
        <property type="gene ID" value="DCAR_003943"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205519"/>
    <dbReference type="OMA" id="HCKMATS"/>
    <dbReference type="OrthoDB" id="423598at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3460.10:FF:000001">
        <property type="entry name" value="Chlorophyll a-b binding protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19 20" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="6 20" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15 20" id="KW-0157">Chromophore</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 20" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="12 20" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="18 20" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="9 20" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 20" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>3</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KZN11287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG85017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="28041" checksum="8E15E17640B60FC7" modified="2016-07-06" version="1">MAAASMALSSPSFAGKAVQVAPSSSELFGNGRVSMRKTVKAPVSDSPWYGPDRVKYLGPFSGEAPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHSRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWATQVILMGAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEAFAELKVKELKNGRLAMFSMFGFFVQAIVTGKGPLENLADHLADPVNNNAWAFATNFVPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166INW7</accession>
    <name>A0A166INW7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_003985</name>
        <name evidence="4" type="ORF">DCAR_0104246</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166INW7"/>
    <dbReference type="Gramene" id="KZN11329">
        <property type="protein sequence ID" value="KZN11329"/>
        <property type="gene ID" value="DCAR_003985"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007875365" evidence="2">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN11329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="86" mass="9442" checksum="6B9E8BEC2DF64E62" modified="2016-07-06" version="1">MASSRALLALLLILAFSFSSARCRKVLIIHEGVYVHPLHEEPAATTTPSKRGQAFIMNENLVDQQLKSISRRHDQSVPSPGDGHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IWJ4</accession>
    <name>A0A166IWJ4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_004223</name>
        <name evidence="3" type="ORF">DCAR_0104517</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IWJ4"/>
    <dbReference type="Gramene" id="KZN11567">
        <property type="protein sequence ID" value="KZN11567"/>
        <property type="gene ID" value="DCAR_004223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007875502" evidence="1">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN11567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="83" mass="8968" checksum="41C1E95D4E5832BF" modified="2016-07-06" version="1">MALFSMFLGCFLDSSPQVAVEGDDKTASAAAYSLDISENNCESKSHSDQDFSSGDVEKEINNKSKGSQILVSYFPTGSRLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J7V5</accession>
    <name>A0A166J7V5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104918</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J7V5"/>
    <dbReference type="Gramene" id="KZN11886">
        <property type="protein sequence ID" value="KZN11886"/>
        <property type="gene ID" value="DCAR_004542"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213769"/>
    <dbReference type="OMA" id="TESNAMM"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="7"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="7">
        <location>
            <begin position="338"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG85726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="566" mass="62285" checksum="BC13C757DC7232CB" modified="2016-07-06" version="1">MAGQSRKWMILVVTIWLQAFTGTNFDFSAYSSEFKSVLGVSQVQLNYLAVASDLGKALGWSSGLALMYLPLWLVMFMSAFMGLVGYGLQWLVISNIITLPYFAVFLLCLLAGCSICWFNTVCFVLCIRNFPTNRPLAISLTVSFNGVSAALYNLAAKAFDSSSNDTYLLLNAFIPLVTSIAALVPILRQPSLDPLPDDAVKRDQLIFLLLNFLAVTTGFYLLFIPSTDPSSARLLFCGAIALLILPLCIPGMVYARDWFHHTIYSSFRVEGSGFILINHVDLELHKEILSRDNSLVDNASYVDSGDGYYDLLDGSETDSSEGCCKTLITRDRLAMLGEEHKAKMLVRRMDFWLYYFVYFFGGTIGLVYTNNLGQIAQSLGLSSTTSTLITIYSAFSFFGRLLSATPDIIRTKFYFARTGWLAIALLPTPIAFFLLAASGSEVALQAGTAIIGLSSGFIFAAAVSVTSELFGPSSVGVNHNILITNIPIGSLLYGLLSALVYDANASPDIEMMPNSLVCMGRKCYFLTFVWWGCVSVLGLASSALLFLRTRSAYDHFEKNRRSELLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WA43</accession>
    <name>A0A169WA43_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_010613</name>
        <name evidence="8" type="ORF">DCAR_0312071</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WA43"/>
    <dbReference type="Gramene" id="KZN01859">
        <property type="protein sequence ID" value="KZN01859"/>
        <property type="gene ID" value="DCAR_010613"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN01859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="108" mass="12875" checksum="323AF61B841C4FCB" modified="2016-07-06" version="1">MDELCMCRLLVVERTAWTENNAGRRFVSCVKGRNGCNIFRWTDPPVCARGRAVICGLLRRIERNEEELEKLMLLQEEKSNCRVQKFRCLNVKMFVFIVVVIWFVWTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YAI7</accession>
    <name>A0A175YAI7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0205252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YAI7"/>
    <dbReference type="Gramene" id="KZM80569">
        <property type="protein sequence ID" value="KZM80569"/>
        <property type="gene ID" value="DCAR_032099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="16577" checksum="814EA02B28324E96" modified="2016-09-07" version="1">MSRSLDECDAILDNMCDCYRMSVEKTRWYGDAIGRRYRECPEGVCGFHKWVDPPLCTRGQEALRELQRRHEVSYEDDCRRRDALLAWYDRRLEDERKKFTESLAGLSLLCDVVKNLVLEASDPVEPVEPAPSLYPPMEEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDA1</accession>
    <name>A0A175YDA1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_029217</name>
        <name evidence="9" type="ORF">DCAR_0933624</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDA1"/>
    <dbReference type="STRING" id="79200.A0A175YDA1"/>
    <dbReference type="Gramene" id="KZM81604">
        <property type="protein sequence ID" value="KZM81604"/>
        <property type="gene ID" value="DCAR_029217"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202168"/>
    <dbReference type="OMA" id="FHKSETC"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM81604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH14108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="312" mass="33827" checksum="FC2E3C26089BE51E" modified="2016-09-07" version="1">MERIYIDNNYNLRKVMALSGVVSLINNSPVTLYNHSLRNSGFDSRADCGVLSSLENLDSPIVRYYRSGSPSSKSPIVFTTPVKVEEDVVVMDGVLVGPVSGGRHRSLSMSDSGGSSSSAGKGFYKMEMCRTWEDFGSCRYGAKCQFAHGKEELRPIRFSNKSKLEAQICKSYTSGSCSYGSKCRFIHQQNNSPESAKDSPFTVISSTPQTVSQSDSSASATDSPVSVISSKTRTVSPIKLENAMNTSESSSFTFAKTDWSPLDDGIDVVLPCSSSTDKAPRGEIDAYINSVLYGPSKRMKLPVFAAICPSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFC8</accession>
    <name>A0A175YFC8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0934619</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFC8"/>
    <dbReference type="Gramene" id="KZM82309">
        <property type="protein sequence ID" value="KZM82309"/>
        <property type="gene ID" value="DCAR_029807"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202729"/>
    <dbReference type="OMA" id="EKQIRNC"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000177">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000017">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043500942" evidence="12">
        <location>
            <begin position="24"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="610"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="628"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH15084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="647" mass="71804" checksum="33BC8C17E9A41984" modified="2016-09-07" version="1">MKRWWSMIVAVVALVLVVESVMAQQKNDTILNSGCSQYNASSTLEFFRNLNTTLADLRREITVNRTYFGTQQQARTTNPVYTMFQCRKYLSTAECVSCFDRAALDIRSICKLANGARVIYDDCFLRYESNGFYTTATALGNTGRCNNDTVANSTSANFNTAAGDVLNNLLLATPKIKGYYAATTEQVVGSNTTVYAVAQCAETVTETECQNCMNVAYENIKSCPPRSGARAIDAGCFMRYDDSAFFASNETTNIKKYLKDGGSSDKKAIIGGIAGGVGLLLILLALLLWYRLSGKKKVAQRGHILGSTELQGPVTYNYKELKSATKNFNEEYKLGEGGSGDVYKGIVKNGNIVAVKRLALSTTKAKASFESEVRLISNVHHRNLIRLLGCSSKGPDLLLVYEYMENGSLDRFLYGGKRGTLNWKQRFDIIFGTARGLAYLHEQFHVRIIHRDIKPGNILLDDELQPKIADFGLARLLPEDQSHLNTKFAGTLGYTAPEYALQGQLSEKVDTYSFGVVVLEIVSGRRCSDTNIESDTDFLLEYAWKLHEKDMHLKLVDETLDPNDYTTEDAKKIIEIALMCTQSPASLRPTMSEVVVLLLSDDRSLEQRPLSKPTFVHSENRIREDTHSTPPQSVSNATATLSDFVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YG80</accession>
    <name>A0A175YG80_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0935064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YG80"/>
    <dbReference type="Gramene" id="KZM82619">
        <property type="protein sequence ID" value="KZM82619"/>
        <property type="gene ID" value="DCAR_030188"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200497"/>
    <dbReference type="OMA" id="CLCDLRP"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00322">
        <property type="entry name" value="FNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215:SF1">
        <property type="entry name" value="F9L1.8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00410">
        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH15523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="259" mass="28293" checksum="382D1CF482358CB9" modified="2016-09-07" version="1">MTSSTLLLRRVLSTAAALRQTSASLHPVSLSSISPAAESLFHVSLDVSSSPSLVSSYTRPGQYLQLRLPHNNRSTLLAIASPPSLAESSGVFEFLVKSVRGSTAEMLCELKEGDIVELSHAIGNGFEVEEIRRCETVFIFATGSGISPIRSLIETGFNADERSDVRLYYGARNLIRMAYQDRFKDWESSGVRIVPVLSQPSDNWTGERGFVQAAFSRSKKIYNPHSTGAVLCGHRQMAEDITSALVSDGVSIRKILKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGP3</accession>
    <name>A0A175YGP3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DCAR_029605</name>
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        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="26"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH11001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="326" mass="36254" checksum="3CABFD146EEA5C5A" modified="2016-09-07" version="1">MGDLKLVIQKGPRQGETLEFRPGSIIRLGRVVRGNSVAIKDSGISTKHLEIQFESDLGKWVITDLGSSNGTVLNGSVIDVKVPVCLSNNDVVKLGEVTLILVKICDEVAVGKGIEGFGEVENVVEKPKPRRGRKKEIGGGKGNENVVAVEVNQGRQLRSRVTGNAINKEGGKFEDFGKQLESLAAVERKMHKGRGKKKVVKEEPEIEVEEDLVKALPELVLDRAVKESTINEFADMSRVDDVLEMKDIEQKEGSGVKNGDFNLQVEPDLEKMTLGEWFDFLEVYLPKKIHDETEELIFEMKERAKQFHEYSVQQKRAMGKGKSLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQC8</accession>
    <name>A0A175YQC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_026915</name>
        <name evidence="13" type="ORF">DCAR_0832661</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQC8"/>
    <dbReference type="STRING" id="79200.A0A175YQC8"/>
    <dbReference type="Gramene" id="KZM85663">
        <property type="protein sequence ID" value="KZM85663"/>
        <property type="gene ID" value="DCAR_026915"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197048"/>
    <dbReference type="OMA" id="NFVQFGR"/>
    <dbReference type="OrthoDB" id="2015537at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="97"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM85663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH13152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="424" mass="47460" checksum="BC838EA44C547EB1" modified="2016-09-07" version="1">MGVIDICKGSTCLIKPQIKLTFGHSDRPNCCSFERSQKSSWVSCMASTVPLNDVKEGQKQHLTGDSFIRPHLRKLSPYQSILPFEVLSTRLGRKPEDIIKLDANENPYGPPPEVFEALGAMKFPYIYPDPESRRLRAALAEDSGLEDKYILVGCGADELIDLIMRCVLEPGDKIVDCPPTFTMYEFDAAVNGALVIKVPRKSDFSLDVEMIAKVVEQEKPKCIFLTSPNNPDGSVIHDDALLKILHLPILVVLDEAYIEFSGMDSRMQWVKNHENLIILRTFSKRAALAGLRVGYGAFPLSIIEYLWRAKQPYNVSVAAEVSACAALSNPTYLETVKVALLQERERLFNLLEKVPFLNPYPSHSNFILCEVTGGKDAKKLKEDLSKMGVMIRHYNNKELSGYVRVSVGKPEQTDVLMNCLNTMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W415</accession>
    <name>A0AAF0W415_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100867</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W415"/>
    <dbReference type="KEGG" id="dcr:108217115"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF246">
        <property type="entry name" value="TRANSCRIPTION FACTOR, TCP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="7">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="18531" checksum="EE2EAE9EBE2BED29" modified="2024-05-29" version="1">MSSQKDFNDDSQVSGGPSQPAPRKDLHTKVEGRGSRIRMPTRCAARVFQLTKELGLATDGETIQWLLQQAELAIIRATGTGTVPATATVTADGSLRVPETKEGAAEDVSKTSGLAPVGPSTVSVPGYGMAAENGMKMNQNQTSVSCEARASSAEEEGYDEMGELTLNEESVENLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W455</accession>
    <name>A0AAF0W455_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0100393</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W455"/>
    <dbReference type="KEGG" id="dcr:108218462"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="54"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG81248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="483" mass="54570" checksum="512565A6B96CBDD1" modified="2024-05-29" version="1">MGAKLTCCWEPEHNGGSDNHHLEHEDSGESGDLPSFRQYTVEQLRIATSGFAVENIVSEHGEKAPNVVYKGKLENQRRVAIKRFSRSAWPDRQQFLEEARAVGQLRNHRLANLLGCCCEGDERLLVAEFMPNETLAKHLFHWESQPMKWAMRLRVAMYLAEALEYCTSKGRALYHDLNAYRIVFDEDANPKLSCFGLMKNSRDGKSYSTNLAFTPPEYLRTGRVTPESVMYSFGTLLLDLLSGKHIPPSHALDLIRDRNLEMLTDSCLEGQFSNDDGTELVRLASRCLQYEPRERPNPKSLVTALIPLQKETEVSSHELMGIPDSTAAFPLTPLGDACLRKDLTAIHEILEMLGYKDDEGTATELSFEMWTNQMQETLNSKKKGDVAFRHKEFSAAIDYYTQFIDVGIRVSPTVFARRSLCYLMTERPLEALNDAMQAQVISPVWHIASYLQATALSTLGRENEGQVALKEGTVLEGKRDENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W484</accession>
    <name>A0AAF0W484_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W484"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042161727" evidence="1">
        <location>
            <begin position="23"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="162" mass="16796" checksum="21BF047A2397DEF1" modified="2024-05-29" version="1">MAFSHAMLMLTFSLTFVVLSSGQAPAMSPSTTMPPMMMTPPPMVTPPPMMMTPPPMMMTPPPMMMPAPIASPPEMMTPPPVMMTPPPMMMPAPMATPPEMMMTPAMSPDSMSSPPAPMGPAMAPTPTMTGMSPVESTPGSAAFKQGSSLAMLALLGGAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4C8</accession>
    <name>A0AAF0W4C8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0100499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4C8"/>
    <dbReference type="KEGG" id="dcr:108204806"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF2">
        <property type="entry name" value="OS10G0484300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="500"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="570"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="72910" checksum="1AF1650E8A71308D" modified="2024-05-29" version="1">MCSSLLKSIKIASFLHPSCNFYCISRPRFSSSSLATIHDSQPSSISSSLYSFLPHTQNPKNIVDMICSSFRKNGEKPNFSILEKDSKWLISHLGSQEISRVLLRCQSDSDTALCFFNWVRNDLCVSPNVHNYCVIVHILACSKNFKQAMVLLLEVVELSRGVSGCSGDSDVFKVLVLCTNECSWEPGVFEMLVKAYVKKGMVREGFVAFEEMLRFGLVPSVVTVNCLMNGLAKLNYVDRCWYLFEEMRRIGIHANCFTFNILTHVLCKGEDVDKVNKFLDEMEEEGFIPDVVTYNTLIDSYCKKRRLKDAIYLFNIMYRRGVLPDLVTHTALINGYCRDMNMREARKLFNCMIQEGLCPDVTCYNTLIYGYCKEGMMQEARTLLRDMIGNGVRPDNFSCWILVKGYEKQDRVLSALNLLVELQRFNVSIPRDIYNYLIVALCKDNRAMAAKNLLERMSRDGQETTEKIFNEIIICLCKRDYVEEALTIKADMVYKGFKPSLVTYRAIICCLCRSTRNVEGESLMREMIESGFLPDVEIFRALVKGFCAERNVYKAELLLSFFAVEFHIIDTECYNVLFKVLSEDGDIAKLIDFQDNMLKLGISPNVMTFRHIIDAMSKNMGSDTNNVCANQECKALVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W552</accession>
    <name>A0AAF0W552_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-terminal processing peptidase</fullName>
            <ecNumber evidence="9">3.4.21.102</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0102376</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Protease involved in the C-terminal processing of the chloroplastic D1 protein of photosystem II. This proteolytic processing is necessary to allow the light-driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.</text>
            <dbReference type="EC" id="3.4.21.102"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.102" evidence="9"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W552"/>
    <dbReference type="KEGG" id="dcr:108204704"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009543">
        <property type="term" value="C:chloroplast thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06782">
        <property type="entry name" value="cpPDZ_CPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.44:FF:000002">
        <property type="entry name" value="carboxyl-terminal-processing peptidase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000043">
        <property type="entry name" value="carboxyl-terminal-processing peptidase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.44">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32060:SF7">
        <property type="entry name" value="CARBOXYL-TERMINAL-PROCESSING PEPTIDASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32060">
        <property type="entry name" value="TAIL-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="203"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG83202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="520" mass="56917" checksum="A5B5A88225BB2CAA" modified="2024-05-29" version="1">MVLLGCSVRPGNSSLLLSSNPNFSYKVHSNSFVVSSVEIKIWNCLSCVRLNASVRQQRFVIQSVRRDRSSHLGLHGIYFRNSGKFSARVRRSVKSLVSFRERFPVIVLQFVVGVMLFMAVAVPVSKSPSWALTEENLLFLEAWRTIDRAYVDKTFNGQSWFRYRENALRNEPMNNREETYVAIRKMLGTLDDPFTRFLEPEKFKSLRSGTKGALTGVGLSIGYPTKNDSSPPGLVVISSAPGGPANRAGVLSGDIIMAIDSTSTETMDIYDAAERLQGTDGSAVELKISSGPTVRHLSLRREKVSLNPVKSRICETSGAGKDASRIGYIKLSSFNENASGAVREAIDDMRRNNVSAFVLDLRDNSGGLFPEGIKIAEIWLDKGVIVYICDSRGVRDIYDTDGSNAIAASEPLVVLVNKGTASASEILAGALKDNKRAVLLGEPTFGKGKIQSVFELSDGSGMAVTVARYETPAHTDINKVGIIPDYPLPASFPKDEDYLCGCLQDPISSCFLDRVGLFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8W9</accession>
    <name>A0AAF0W8W9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0104651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8W9"/>
    <dbReference type="KEGG" id="dcr:108207027"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="6">
        <location>
            <begin position="44"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="341"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG85462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="57585" checksum="1891006BFA08435E" modified="2024-05-29" version="1">MSVNCIRNLQFLQSIENFLKSPWLMQTCSYSSFKKSKNDPLQDRRMIDRIRLSAKGGDGGNGCYSIRRSRHDRRGKADGANGGRGGDVILECSPRIWDFSSLKHHINAQRGGNGASKNMIGSRGPDKVVLVPVGTVIHLVDGEIPVMVENNSSPDLEPWDIPSTVDVTSKTTEQSSNNLSSQLMQTKYNNTLVNSANTKKASQSPSNDSISKFPDISNKTWDEDSESNIKTLGSGTEPNIVGDNISETELGEDIEEEEQVRYDVAELTVPGQQIIIARGGTGGLGNLCYPKAFKNPKLLKLGEQKNKGVDDIESEANGHSSLSLGMPGSHAVILLELKSIADVGLVGMPNAGKSTLLGSLSRARPTVGHYAFTTLRPDLGNVNYVDFSVVVADIPGLIKGAHENRGLGHAFLRHIERTQVLTYVLDLAAALDGKTGVHPWEQLKDLILELEFHRVGLSSRPSIIVANKMDEPGADKVYEELKRRVPGVPIFPVCAVLDEGVEELRSSLRKLVIGEESHRLQLDNIELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDB8</accession>
    <name>A0AAF0WDB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0205379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDB8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12288">
        <property type="entry name" value="RRM_La_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034878">
        <property type="entry name" value="La-rel_plant_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF62">
        <property type="entry name" value="LA-RELATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="9">
        <location>
            <begin position="116"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="214"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG86178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="47546" checksum="792D419941C7119C" modified="2024-05-29" version="1">MAQAHPPEKIKENPDQLEINSGKMKEITCSTSGGSSSSNNTSVPFKFNVQAPEFVPSAYNNNSNSPTHMPVSGYFYPCFHYISGNDGAADWVYVGDQDSLQLVSSPPPEITNYAKDVLTAELKQKIIKQAEYQFSAMSLLANESLIKQINKDPEGYVPISFIASTKKIKSLVSNSHSLAQALRSSSKLVVSSDGKKVKRKHPFTEKDKEDLQSRTIVVENLPEDHSHQNLEKIFNVVGSVKTIRICHPPDVNSPRPKGNIIISNKLHALVEYETPELAERAVDKLNDERNWRKGLRVTLLLRRSPKSVLKNRKSEFDGYFDDDCDEDDTPGQGIPENSSQPISLESAVDNLAEESSAAAKKGWARGRGKSRLRAPIQTGRGSLLSSPMSSSSPLCEGSSTKQATKGPRMPDGTRGFTLGRGKPICTSSAAISKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGB3</accession>
    <name>A0AAF0WGB3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208385</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of target mRNAs. Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of CLV1, PNH, WUS and FAS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGB3"/>
    <dbReference type="KEGG" id="dcr:108209374"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012940">
        <property type="entry name" value="NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF128">
        <property type="entry name" value="PUMILIO HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07990">
        <property type="entry name" value="NABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="701"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="721"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="757"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="793"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="829"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="865"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="902"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="938"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="974"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1055" mass="114726" checksum="23D88C8644BF75D7" modified="2024-05-29" version="1">MLSELERRPVHSGNEGSFGDDFEKELGLLLREQRRNQDSDDRERELNLYRSGSAPPTVEGSMNAVGGLYNNGGGGSVFSEFDLNNKSGNGFSSEKELRSDPSYLSYYYSNLNLNPRLPPPLLSKEDWRYAQRLQGGSSTVGGIGDRRKVNKNENSNGGGGVSLYSKPPGFNANKQKSEGELENQAPAGWGVDGLIGLPGFGLGSKEKSLGGIFQDDLGRVTPTSGHLSRTPSRNTFTESVDTLGMAEAELSQLQQDLASADNIRTSTKVQNSSSGHNGGPPSSYSYAAALGASLSRSTTPDPQHIARAPSPCPTPIGGGRAGNSERRGFSSNTCNDVHSNISEPTDLATALSGMSLSNGVMNEEDCLASHIEQIVDDHENYLFNMPGGQNNVNQQSYLKKSELGQLNMPSPHSTKLSHSISEMGNGDGYGYSSSSTQADLQRTAATSAYQKGFPASLFNGGGGFGSNYQQTDGTNSSFTNYGGSGYPLNLPMQSMMSSHLGNSNMPPLFENATAASAMAMPGMDSTMMGGTFTSESHPNYAALDSQNLGRIGNQMAGSALQAPFVDPAYLQYLRTAEYAAQASINEPSVESNYLNNSYLDLLQKAYLGSLLSPQKSQYGISLGSKTAGPHGYYGNPAYGVGLSYPGSPLASPVLPHSPVGPGSPIRHGDVNKRFHSGMRNVAGGGIMGPWHLDASNMEHSFASSLLEEFKSNKTRCFELLDITGHVVEFSADQYGSRFIQQKLETATTEEKNMVYQEISPQALALMTDVFGNYVIQKFFEHGMPSQRRELANQLLGNVLTLSLQMYGCRVIQKAIEVVDLDQKIEMVGELDGHIMRCVRDQNGNHVIQKCIECVPENHIQFIITTFFDQVVTLSSHPYGCRVIQRVLEHCEDQKTQDKVMEEILACVSMLAQDQYGNYVVQHVLEHGKPHERSTIIRELAGKIVQMSQQKFASNVVEKCLTFGDPSERQLLVTEILGTTDENEPLQAMMKDQFANYVVQKVLETCSDQERELILSRIKVHLNALKKYTYGKHIVARVEKLVAAGERRAAQSQQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ04</accession>
    <name>A0AAF0WJ04_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homogentisate phytyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0208679</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJ04"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13960">
        <property type="entry name" value="PT_UbiA_HPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044502">
        <property type="entry name" value="AtHST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009525">
        <property type="entry name" value="PRK12887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009:SF7">
        <property type="entry name" value="HOMOGENTISATE GERANYLGERANYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009">
        <property type="entry name" value="HOMOGENTISATE SOLANESYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG89441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27277" checksum="EF8B194BD5A8DAD2" modified="2024-05-29" version="1">MNIYVVGLNQLFDVEIDKVNKPNLPLASGEYSMGLGKAIVSACGLMSFAMGIMFQSPPVFFALLICFLFGSAYSVELPFLRWKRNAFLAAISILTVRAITVNLAFFYHIQKYVLGRPMVFPRSLCFATVSISLFTTVIALFKDIPDVDGDRDFGIQSLSVSLGQKRVFWLCIGILLIAYASALVIGASSSFLLSKLVTVIGHCTLASVLWLRANSVNLKDNASVTSFYMSIWKLFYAEYLLIPFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJS2</accession>
    <name>A0AAF0WJS2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0208881</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208822"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902417">
        <property type="term" value="F:(+)-abscisic acid D-glucopyranosyl ester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000024">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="557"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="910"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="953"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1030"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1056"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1139"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="303"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="614"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="919"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1238"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="864"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="871"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG89643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1623" mass="182076" checksum="359486B8B2C581AF" modified="2024-05-29" version="1">MAFGPLVWYCRPASDGVWAKAVQNAFGAYTPCADSMVVFISYVVLLGLCLYRIWRIMTDLTVQRFQLKSKYYNYMLGLWAAYCTAEPLFRLIMGISVVNLDGETTLAPYEVVSLIVKALAWCSMLVMICVEMKVYIHEFRWFVRFGVIYVLVGDVVMLNLMLSMSDFYTRSTLYLYISEVVIQGLFGLLLLVYIPSLDPYLGYSPIQTESADNDEYEELPAAENICPERHVNIFSNIIFSWMNPLMQLGYKRPLTEKDIWKLDTWDQTETLNEKFQKCWAEETRKPKPWLLRALNKSLGGRFWWGGFWKIGNDLSQFVGPMLLNKLLESMQNREPAWIGYIYAFLIFVGVVCGVLVEAQYFQNVMRVGYRLRATLIAAVFRKSLRLTHESRRKFATGKITNLMTTDAESLQQICQSLHTLWSAPFRIVIAMVLLYQQLGVASLLGALLLVLMFPIQTYVISKMQKLTKEGLQRTDRRIGLMNEILAAMDTVKCYAWESSFQSKVQDVRNEELAWFRKAQLLGACNSFILNSIPVLVIVVSFGLFSLLGGVLTPAKAFTSLSLFAVLRFPLFMLPNIITQVVNANVSLKRLEELLISEERMLLPNPPVEPGLPAVSIKNGFFSWDSKSEKPTLSNVNLDIPCGSLVAIVGSTGEGKTSLISAMLGELPAVADTSVVIRGTVAYVPQVSWIFNATVRQNILFGSVFEPSRYSRAIDVTALRHDLDLLPGGDLTEIGERGVNISGGQKQRVSMARAVYSDSDVYIFDDPLSALDAHVARQVFEKCIKEELKGRTRVLVTNQLHFLSQVDRILLVHDGMVKEEGTYEELSNNGILFQKLMENAGKMEEYVEEEEEGVDKESQTLKPVANGVTNDVPKDGSQAKKSKEGKSILIKQEERETGVVSLKVLARYKNALGGLWVVMILFTCYVSTEVLRVLSSTWLSIWTDESTPKNHGPGFYNLIYSLLSFCQVLVTLANSFWLILSSLYAARRLHQAMLNSILRAPMVFFHTNPLGRIINRFAKDLGDIDRNVAPFVNMFLGQVSQLLSTFVLIGILSTMSLWAILPLLLVFYAAYLYYQSTAREVKRLDSISRSPVYAQFGEALNGLSTIRAYKAYDRMANINGNSMDNNVRFTLVNMSGNRWLAIRLETLGGVMIWLTATFAVMQNGRAVNQEAFASSMGLLLSYALNITSLLTAVLRLASLAENSLNAVERVGTYIELPSEGPSVIDSNRPPPGWPSSGSIKFEDVVLRYRPELPPVLHGLSFKIPPSDKVGIVGRTGAGKSSMLNALFRIVELESGRIIIDDYDVSKFGLTDLRKVLGIIPQAPVLFSGSVRFNLDPFSEHNDADLWESLERAHLKDVIRRNSLGLDAEVSEAGENFSVGQRQLLSLARALLRRSKILVLDEATAAVDVRTDALIQKTIREEFKSCTMLIIAHRLNTIIDCDRILLLDAGQVLEYDTPEELLKNERSAFSKMVQSTGAANAEYLRSLVLGGDGENRSQREETKRLDGQRKWLASSRWAAAAQFALGVSLTSSTNDLVQLELLDDENNILNRTKDAVITLQGVLEGKHHKIIEETLEQYNVPGDRWWSSLYKMVEGLAIMSRLGRSRLNSEGGFEDKAIDWDGIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM19</accession>
    <name>A0AAF0WM19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cation/H+ exchanger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0311640</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM19"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF26">
        <property type="entry name" value="CATION_H(+) ANTIPORTER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="48"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="12">
        <location>
            <begin position="483"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="13">
        <location>
            <begin position="627"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG92375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="87077" checksum="69DFC6309EE56178" modified="2024-05-29" version="1">MSEARQRAAGMVCYAPKMILSQGILEHENPLDYSVPLFVVQLLVVSCLSRVIHLVFKPFRQPKALSQILCGVILGPAILGRSSVILEALFPLRGVVVYDTMANIGLVFNLFLIGVEMDIFHSFRMERRSITIAFVGMLLPFAISCSISYILEVHLPKGLNHTNYIIFVALAASVSAFQLLSRQLVELKILNTEIGKTALQSSILNDCFSWIAVIVFLVVAKNDGVSSSPIWLLLCSVAFVLFCIFLVRPAIWLLIKNTPEGESFSELSICLIFSGVMMAGFISEAIGMHAIFGAFVFGLVIPSGPLVQVLIERLEDIVTGILVPLYYASFGIKAKTDEFGDGQHWALLLIIVFAFIAKAIGTFLTALFYRMPLRDAATLATLMNAKGLIELIILNQGLEHEIIDACSFTIMLFATLFTIGITLPLVSIIYKPSRKLVTYRGRAIDKAKSETELRLLVAVQSSRNVPTIINFLDASNTTTGLPLCVYVLHLAELTGRASAMLIAHGTQEKGMAPLNKAQAQSEQILQEFKKLEEQSRCISIQPLTAISPYATMHEDICNLAEDKKVAFIMIPFHKQQTVDGEMTTTNPEIQKVNQRVLENAVCSVGILIDRGLTGISPMAGTELAHHIAVLFFSGPDDREALSLGMRMAQHPNNSVTVIRFIPGKQAAMEGSEHRHIPRRNHRPTPMDTEKFLDDDYIREFRLLEARDESLYYAETVVNDSEETVAAVKSVDNIHDLFIVGRRQGTLSKLTDGLTEWSETPELGAIGDLLVSPDFPARVSVVVVQHYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN06</accession>
    <name>A0AAF0WN06_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN06"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="34358" checksum="CA1E78F7A998744D" modified="2024-05-29" version="1">MSPVNITLIAAHRGEYISDDKDENVQEILAFCQLLLKHFANSYLSIKHSSVGDNNVVEIGQSGDTPVYHKACSKKNKMYRNNVKSNLHQFVMDPLGLKRRPLSPLSPSSLRERQRRRCGAHDSTDGNGSLLCKENQNPLRKAQTFGSGGNTIPGILHKGSLKTSQCSGPSTAESVVNSENQHKYLKETCPAERKSFLGNRGGLTNRVFAAVTQRTPLARLIAIQSNKQSVSNRFQPTIFNCFQISSLKASQCSVASTADFIFAHNFFSYLMLLTAMTKLALSTPSDLQYNKPGSVGHASDGGFLLSITSLYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNT6</accession>
    <name>A0AAF0WNT6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0310938</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNT6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000014">
        <property type="entry name" value="Kinesin-like protein KIN-7G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7K, CHLOROPLASTIC ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="65"/>
            <end position="384"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="909"/>
            <end position="933"/>
        </location>
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        <location>
            <begin position="386"/>
            <end position="473"/>
        </location>
    </feature>
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        <location>
            <begin position="733"/>
            <end position="816"/>
        </location>
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        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
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            <begin position="33"/>
            <end position="43"/>
        </location>
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        <location>
            <begin position="559"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="145"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG91688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="933" mass="103970" checksum="048D520C732E480C" modified="2024-05-29" version="1">MASKPGLRSQKLSSRASSSTTSSSKQFPEASADDLSSPASSTARSKAKHYSAETVSVNARKLKENVTVTVRFRPLSPREISQGEEIAWYADGESIVRGEHNPSIAYAYDKVFGPTTTTCHVYDVAAQHVICGAMEGINGTIFAYGVTSSGKTHTMHGDQRSPGIIPLAIKDTFSIIQETPSREYLLRVSYLEIYNEVVNDLLNPAGQNLRIREDAQGTFVEGVKEEVVLSPAHALSLIAAGEEHRHVGSTNFNLHSSRSHTIFTLTIESSPCGENDEGEAVNLSQLNLIDLAGSESSKAETTGVRRKEGSYINKSLLTLGTVISKLTDGKASHVPYRDSKLTRLLQSSLSGHGRVSLICTVTPSTNNSEETHNTLKFAHRAKHIEIQAAQNKIIDEKSLIKKYQHEIRTLKEELEQMQRGVAAIPQLKHSDGDDLVTLKQELEDGQIKLQSKLEQEEDAKAALLSRIQRLTKLILVSTKSSQSIFPHRPGQRRRHSFGEEELAYLPFRRRDLIVDDEQIDTCTTLDQNSETADETLKEGKTTRKNGLLNWLKPRKRDSSGTFTSTSDRSSGTKSVSTPSTPKAESGNLLAESRLSHSTPTENTPSADLLLSIKHERETPEESSQQVSPLQLTNCIVSYVNCIKSVAELETQLNEKSFELEVKIADNRIIQEQLTQKIHECEKLQQTVLTLRDQLSDAQDLKKFSHVIGNSDRYDETQKNHRELSTEKETAVSKDKYEGLFLKAQMEIAELTEKLTNLTESREKLSIQNQKLAEESSYAKGLASAAAVELKALSEEVAKLMNQNEKLASELAEQKNSQIQRRTIAANRTGRRDVNLRRHEQGTVNSVSKRELALFRERELSYEAALAEKDQREAELHMRVEESKQKEAYLENELANMWVLVAKLKKSQGAEVNASDITRGPISDNDYEHWNSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRV5</accession>
    <name>A0AAF0WRV5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">cytokinin dehydrogenase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DCAR_0313532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2</text>
            <dbReference type="Rhea" id="RHEA:13625"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15825"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="EC" id="1.5.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.12" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF127">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="61"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG94239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="517" mass="58431" checksum="C18B6907551BFFBF" modified="2024-05-29" version="1">MSFLISSLISVLGRYLNPLISIKSSSSIPNHGTLSVKLAPKLHFTSDAIKMASTDFGKIITSNIPSAVLFPSHIDDIKNLITLSYNSTTPFPIAARGHGHSVRGQATARDGVVVDMKSLSNSHVGTRVSWTSSLGYYADVGGEQLWIDVLCATLEHGLAPVSWTDYLYLTVGGTLSNAGMSGQAFYYGPQISNVLELGVITGKGEFFTCSKHTNSELFYAVLGGLGQFGIITRARIVLDKAPKRVKWVRMLYHDFSAFTRDQEHLISITGLDYVEGHLLMNQSSANNWRSSFFSPNDVSNIASLITQHKIIYCLEVVKYYDDFTTHTIDKELKVLLEGLSFNPGFVFEKDVTFMDFLNRVRSGELQLQSQGLWDVPHPWLNLFVPKSRIFDFHQGVIVNMIHARNITRGLVLLYPTNRKRWDDRMSAVTPDEDIFYSVGLLCSGDNDNWKAMDEQNKDILEFCEKAGIRFKQYLPHYTTREEWIDHFGEKWSIFQERKTMFDPKMILSPGQRIFHHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WU41</accession>
    <name>A0AAF0WU41_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plant bHLH transcription factor ACT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0313443</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WU41"/>
    <dbReference type="KEGG" id="dcr:108211543"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
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</entry>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="265"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="322"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="1016"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="794"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1312"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="808"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG97315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1428" mass="157511" checksum="E7212551EB3E4FF8" modified="2024-05-29" version="1">MTGGRCSRRRKGGCDVGIVDSRVPIDFSRENCEIEEIDLGIGKVDLFTQARKALSERCPFDLEDGQAVKTVSLPKEFADLLSRKSDGRKRHKKSHAAESKSTRLAKSKGAAFWARNEEYFRGLTVDDVDKLYQLGSIKFSGDWNCLMIPCVNNVRASSCVSNGNDSNLNNGFGDVSPASYKKEEEEAKDAHDDKLAEVECGEGIELALEEKDRALNQQGSGLEWLLGSRSKVYLTSERPSKKRKLLGGDAGLEKLFVASAVEGSSSLCHYCSLGETGDQLNRLIICSSCSMAVHQRCYGVQEDVSESWLCTWCKNMNQKDLERPCLLCPKQAGALKPVRKKGCGSDSSGFAHLFCCQWMPEVYIEDTRRMEPIMNLEGIMETRHKLICRLCKIRHGACLRCSNGACRASFHPICAREAKHRMEIWGKFGSDDVELRAYCSKHSEIQNNIITLQDGDSRLLIPDPHITKHQPMSTMTHKIKIGRKNEEKVAGHNETSELDLDRVDSSVPHGDVFPNASSNLTNQLAFGDTQQSNNADVLAKKSRDDIDLSECLIDRGRVKMTDVASEIGVSPEVLTSNLIGEHMVPEVQCKIVEWQKNHATIGPSQKNLKVRFKHPTKVEAGSTDETHVLVSESCIPNVSVTSVPPRRRTKNDIRILRDGKALCLTESSSIADGVAMSDKSAHHLAMHEPACKSDTTQKILIEPDGSQDILRNSSSKTEGASLLICTAVKSFQAEGGAVSENSAALGSVVANPVCDTAVNCVPNIIETEAVSISYMHPTIQKTLSQIPNLVVKRPLSDEDDGSRDGEFSSAEASSSSSICCHHQTEDSVSPGSTSKVGGIVEQLAQARRMGILEQSPADEVEGELVYFQQHLLHNAVKRKHFSDNIIVKVLTNLPKETDALAKQKWDAVTANKYLSELKELKRQGRKERRHKEAQAVLAAATAAAAASSRISSFRKDSQEESAQRDIVNSSSGRASLYSQQMLRAKETLSRLGTTRVSIEKNSDAVHSTSDFSKEYPRTCEICRRSETILNPISICSSCKVAVHLDCYRSVKDSAGPWYCELCEEMSSSRSFGAAAVNSWDKPYFLAECGLCGGTAGAFRKSTDGQWIHAFCAEWILESTYKRGQANLVQGMETISKGSETCHICQRKQGVCVKCNYGHCQSSFHPSCAKSAGFHMNLKACGGKLQHKAYCEKHSMAERAKVETQRHGIEDIKSLKPVRVELEKLRLLCERIIKREKLKRELVLCSHEILASNREAVALSAVSYSSLCPTDVSSESATTSLKGYTDDYKSGSEAIQRSDDITVDSTVAGKRRIKLPVSMDNDQKTDDSSTSQQLFPSKPSDRVSFSGKQIPIRPSSAASWSFSSEGEKRAKFRKHPETFEKELVMTSDQASMKNQRLPKGFVYVPIRCLSNEKEAVPGACTQEHIRSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X2S5</accession>
    <name>A0AAF0X2S5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2S5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF70">
        <property type="entry name" value="MAJOR ALLERGEN PRU AR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="3"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="17915" checksum="AF09733A8DDB2EEB" modified="2024-05-29" version="1">MGFASFTDEYTSPIAPSRLFKASIIDSHNLVPKLLPQGIKSIELVQGDGGAGSIKQVNFIDGRNFNSVKYHIDELSEEKYTYNYTLLEGDALVEKLQKITYEVKFESSPSGGTISKVTSKYYTDGDFMLKEEDIKAGKEKVLMMYKVVEAYLLQNPDAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3Z5</accession>
    <name>A0AAF0X3Z5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0520383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3Z5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052259">
        <property type="entry name" value="Nucleoredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871:SF96">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="8">
        <location>
            <begin position="291"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012612"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="594" mass="67779" checksum="7270E7FDBC619934" modified="2024-05-29" version="1">MIGTPSGFATDDELVRVGGWAVMKIEPELTDLMVMTRSLPNNCKADDSVKKHIEKPAKGKKKRNKGKKGNKPYRIGDTVNLKDLLFTQERDYLIKYKDVQIKKEQLVGKVIVLHFVSLVKGHDNLIPDPDLIDIYKKLEPKGGFEVVFVAVGDGLTCLDTRESTSTYSTPQQCFEEIFSTMPWTAIPFSDLKSRNRLEKLFCIVSDTRLEMEPISFVIDSRGVVLQADATQVFSMYGSAGYPFTKQRLKCLDHEDYVTMEQLSVSTLLASPQRDYVITNKGDQVPLHKLEDKVVALYFFTDISNVRITSKLKLAYKELCEKMEAFEVVLINLLSISSDTCEDTFWKTFEAMPWLAIPSKDTDCCKKLLRIFDSTICDPGPYPVSKLVIIGPRGKFIEPCGANILCCYGIPAYPFTRFSAVNLKIEKVKDVKPEMFWNLDAIFRQQNGSEVQFSQIVGKRIIVLFQLFNDIPEQTLKKLIALYNQMKGTDDEFEVIHIREESKWGHVGAVIPWLMHPPFSNASDAGKVMRRLFYYGENGLVAFDRDGRIVRMTRRLAVGKTVFPFFDAEKMEDEVLQDMQEDLYEPLETLLCCCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5N2</accession>
    <name>A0AAF0X5N2_DAUCS</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="1" type="ORF">DCAR_0521650</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="50728" checksum="1BF01239F04035D4" modified="2024-05-29" version="1">MIYIFFFNNEFCCSKPNSLSSYTRLSPYPDTNVSNLVIGIASSSNFWRYRKPYIESWWKPNVTRGFIFLDRAPNEPSSLAPYRISEDTSRYNAFNRRKNPHVIRLVRVVEEIFKADNGDDVRWYVIADDDTILFLDNVAEVLRKYDHSKYFYVGMHSECTDSNALFSFNMAFGGAGYALSYPLARLVAKNLDWCLKRYPTFVGSDQIMHSCISDLGVSLTLEKGFHQIDFHQDISGLLSAHSQSLVLSLHHLDAVDPIFPSMNRLDSVKHLMKAARIDEARLLQQTICYQKHKNWSFSISWGYSIHIYEKIIPLSVLQAPLETFSRWRRPAYWPYYMFNTRPVLNNSCEAPHVFFFQSIERTRENHHETVYIRSSARGLPACSSSGNHSADHISEIRVLAPVRVHEDGSRRECCDSEETYMNSTSFKIRSCMEDEILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7L5</accession>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0521334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X7L5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000024">
        <property type="entry name" value="Methionine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="439"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH01947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="596" mass="67848" checksum="4CB5FB09B494ECA5" modified="2024-05-29" version="1">MEFLVVEDMKKVPIPGKRNMLITSALPYVNNVPHLGNIIGCVLSADVFARFCRLRGYNVLYICGTDEYGTATETKAMEEGLTPKEICEKYHAIHKDIYNWFDISFDEFGRTSTPQQTGICQAIFTKLFKNRWISENTMQQPFCDTCQRFLADRFVEGTCPKPDCSYDSARGDQCEKCGKLLNPAELKNPRCKVCRATPRIRDTDHLFLELPLLKDILVKYINKMSVEGSWSENALKATQAWLKEGLKPRCITRDLKWGVPVPHEKYEDKVFYVWYDAPIGYISITSCHTSEWEKWWKNPENVELYQFMGKDNVPFHTVMFPSTLLGTGENWTLMKSISVTEYLNYESGKFSKSKGVGVFGNDAKDTKIPSEVWRYYLLTNRPEASDTLFTWKDLQAKLNSELLNNLGNFVNRVLSFIAKPKGRGYGSIVPDAPGAETHCPTKALAERVGKYVEQYMDDMEKIKLKQGLKTGMSISSEGNAYLQNTEFWKLYKEDESSCAIVIRTSVGLVYLIACLLEPFMPSFTMEVFKQLNLPPKKVFSLSDKDGDLGQVSRPWEFLPAGHVIGTPAPLFKELTNEEVEHFSRKFAGSQATRAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8R2</accession>
    <name>A0AAF0X8R2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0622912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8R2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="467" mass="50522" checksum="A139373C0FCC5C03" modified="2024-05-29" version="1">MKELWSMALPITGMNCLVYIRAVVSVLFLGWLGSLELAGGALSIGFTNITGYSVLVGLASGLEPVCSQAYGNKNYDLLTLSLHRMIFILLITIIPISFLWINLNSIMLFIGQDPDITSTAASYCIVSLPDLLTNSFLQPLRVYLRSQGVTQPMMWCTFVAVVFHVPLNYVMVVVMKLGVSGVALASVLTNVNMLLFLCGYVYVYGRWEWKWTAGIGGGGVGPLLRLAVPSCIGICLEWWWYEIVTVLAGYLPNPRLAVAATGILIQTTSLMYTVPMALAGCVSARVGNELGAGKPYKAKLAAMVALVCAFLIGIIHVCWTSIFRDKWAGLFTKDEMLKALVASVMPLMGLCELGNCPQTTGCGILRGTARPAVGARINLGSFYFVGTPVAVGLAFWFRVGFTGLWLGLLSAQVACAISILYAVLVSTDWEGEALKALKLNEVEMVEKSENGEENIRFLAKANGNTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9L9</accession>
    <name>A0AAF0X9L9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0522423</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9L9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000015">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000015">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000032">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="10">
        <location>
            <begin position="6"/>
            <end position="150"/>
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                <person name="Iovene M."/>
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                <person name="Moranska E."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <ligand>
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    </evidence>
    <sequence length="410" mass="47022" checksum="42666912C9A3F454" modified="2024-05-29" version="1">MTKSVGADSRRASTDSSELQSERDSSGKGKRLWKKVKYQLVEYHSLPAYLKDNEFILAHYRSEWPIKHIFMSIFTIHNETLNVWTHLIGFFLFLVLTIYTAMEVPKVVDSNTLQHLHDVLRKADMHKLREDLLTCLPSFPTLPDLHNFRDALKNSLPLTDLLTSLSNWHIVELLTNCLPESFSHINHTDNCVLRNVKDDMVSMIAPQTTRQITRWPFYSFMGGAMFCLLASSVCHLFSCHSKRLSYVMLRIDYAGIAALISTSFYPIVYYSFMCYPFFCMFYMGFITILGVATVLVSLLPVFDRPEYRSVRAALFFGMGFSGVAPVLHKLILFSHQPEALHTTGYEILMGVFYGIGVLVYAMRIPERWMPGKFDIAGHSHQLFHVLVVAGAYTHYCAGLVYLKWRDMEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCL0</accession>
    <name>A0AAF0XCL0_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCL0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="136" mass="14537" checksum="2F145E293BEBEB1F" modified="2024-05-29" version="1">MANQQHHHNITNGGGVHDGVFLCFDCNNRPFRSHMDLLRHRLNDHQHQHDAAQAPAPPPPPVNNGIPGDESTDDELIIILDPPPALGEPVAAVPPQSLPQNGPVVDGHAVNEPPQVQPPVENGHAANDRVANGAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCT6</accession>
    <name>A0AAF0XCT6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCT6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="70027" checksum="335E158C5275E360" modified="2024-05-29" version="1">MSGRLMKKVLKEQEAANQQHEKKDLYSDDDSESSNTSPLASSVNPFDLLDDQSQDVRGSSSGYPEVEESLTEKVVLNDTVSQSNAKTKKNKKKKGKEDKILDKKNIESASYKMSNMSMFGEESSSPRYGLSGAIPKNETSGALSSSVLQVEPKMLNAETELRRIFGSKVVNSLGRSHQAGPSRQNRGGRRGNHNHRKTILVSPLEHWPRWDGSFSMEYLETKDHYHYFRYVQSSAYKQAQIAFESAKATHDLNGIANILLYHPYHVDSLITLAEYFKFSGEHQMSADATSKCLYALECAWHPMFSPLQDKCKLKYSHETNRPFFSTLFAHMKNMDRRGCHRCALEICKLLLSLDSDDPMGAMFCIDYFALRAEEYTWLERFSEEFKSDNSLWLFPNFSFSLAISRFCLEGSTDANEAEKTSSTDLMCHALMLHPPVLKKLVAKVPLKDQMWANILNHKFFSIDRTGVPSLDHLINIYVERSYIIWRLPDLQKFLRNSAMKVIDDLDHNRGDAKDWACVREEAFSSEKNEYDHLLVSDFSDSVQTMPPDNLQNFILDPQAMAMQNADQAINPPAVGRAPREVANRNALAVLLESILPWNHYGTSGAGEFEDEHNNDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDL9</accession>
    <name>A0AAF0XDL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0625767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential for the control of the cell cycle at the G2/M (mitosis) transition. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the CDC2 and CDK2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDL9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010332">
        <property type="term" value="P:response to gamma radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20511">
        <property type="entry name" value="CYCLIN_AtCycB-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000032">
        <property type="entry name" value="G2/mitotic-specific cyclin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0195">Cyclin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="217"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="9">
        <location>
            <begin position="289"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="293"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH06341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="46961" checksum="BBA77E692CCA8E5F" modified="2024-05-29" version="1">MASRPVLQQNIEEAVKQKNMAAGEGRNRRALGDIGNLVTVHGIEGKQQQIPRVIRPVTRSFCAQLLANAQAAAENKKQRAVIGDGVVAHKAVHQKKVTVMPKAEDIIVISPDTEEVKKVDRHLNKKKAIEGSSKKKSQTFTSTLTARSKAAAFGITRIPKEQIVDIDAADANNELAAVEYVEDMYKFYKLVEHESRVFDYIDFQPEINEKMRAILVDWLIEVHNKFELMPETLYLTINIVDRYLATKSVARKELQLVGISSMLLASKYEEIWAPEKKILSRLEWNLTVPTPYVFLVRFIKASIPNEPAVENMAYFLAELGLMNYATVMYCPSMLAASAVYGARCTLDTAPFWNETLKLHTGFSEQQLMDCARALVRFHSCAAENKLRVIYRKYSLAERGAVALLPPAKALLNVAASPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDU9</accession>
    <name>A0AAF0XDU9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glucomannan 4-beta-mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glucomannan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0625526</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.</text>
            <dbReference type="EC" id="2.4.1.32"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.32" evidence="11"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDU9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047259">
        <property type="term" value="F:glucomannan 4-beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051753">
        <property type="term" value="F:mannan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06437">
        <property type="entry name" value="CESA_CaSu_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000015">
        <property type="entry name" value="Glucomannan 4-beta-mannosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF64">
        <property type="entry name" value="OS09G0572500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="179"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH06103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="523" mass="60288" checksum="9F97C1BE16660F84" modified="2024-05-29" version="1">MRNTIFHKTEITGSLCDLWKEIRVNLVAPFLYIALWACVAMSLMLFVERLYMTAVILCVKVLGKKRSTQYNVELVLQRMEKNGSHPKLLVQIPMYNESEVYKLSIGAACRLLWPTEQIIIQVLDDSTDQVIKTNVMAECKKWQDQGVNLMYENRANRNGYKAGALREGLQKQYASDCEYVVIFDADFQPEPNFLMKTVPYLVGNNKLGMVQARWKFVNADECLMTRLQEMSLNYHFSVEQEVGSTTCQFFGFNGTAGVWRIRAIKDAGGWKERTTVEDMDLAVRASLQGWKFVFVGDVSVKNELPSTFKAYRFQQHRWSCGPANLFKKMTKEIMLCPRVSVWKKIHLIYAFFFVRKIIAHWVTFFFYCLVIPACVLVPEVHLPKALAIYIPATITILNTGGTPRSLHLLVFWILFENVMSLHRTKAAIIGLLEASRVNEWIVTEKLGNTNQSNKSKVSFFKSRFRCGERIHKLELVMGVFMLYCALYNMQYCNDHMFVYLFLQSGAFFTIAFGFVGTFVPSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFM8</accession>
    <name>A0AAF0XFM8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0626599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFM8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd23431">
        <property type="entry name" value="beta-trefoil_Ricin_AtEULS3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040249">
        <property type="entry name" value="Ricin_B-like_lectin_EULS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257">
        <property type="entry name" value="RICIN B-LIKE LECTIN EULS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257:SF2">
        <property type="entry name" value="RICIN B-LIKE LECTIN EULS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="285" mass="32392" checksum="65313E49967EBCA4" modified="2024-05-29" version="1">MRMVNNIRLNIDAFLNSDGRVQDGTIIGLWEWLQGENQQWKIIPHQYEPSTIDNQTLHVWDYLGRMPSVRVYTKAETNCSLAIRHGKVILAQADPSDPSQHWVKDEKYSTEFKDKHGFPSFALVNKATLQGMKHSVEACHPVQLIPYVPGILDESVLWTLSADLGDGYSAIRMASNTRLSVDAFKNSKGRIHDGTIIGLWEWLKGDNQRWKIVPHQSVPTSIYDHTRQIWGCLSRMPSVRVCTKADTNFSLAVRHGKVILARADPSDLFQVFPCILIYSVIILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIJ7</accession>
    <name>A0AAF0XIJ7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH11818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="337" mass="37756" checksum="8CBB1AE29A8A13E7" modified="2024-05-29" version="1">MSKDVDIISTLPDDLLGRIISLLPTKDAIGTAVLSSRWKNLMDFVPVLDLACHVPTAGFIDTVKKLLPSTSSSANTQVIQNLRLQICECHWCIVPVHVNRWVRSAIERKVVHLDVCLPDFVSGTVANDMILALRIIASDSLQTLNIRGGLGLCMPCSTGSFKNLKTFNLRINNPDKEILANLFCSLPQLEELSVEATFSMIRPGDMNICINIIAPALKQLCLCINQKDYHDVDFKILIDTPMLKYIFLGDDFLAAYLVKSLPCIVIATLAIGMNLYHEDRVFRAIELLRRLSNVKYLSVTSDASAVSEHCLRSPTTISLLDNFQQNSQFYMFYVFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRX6</accession>
    <name>A0AAF0XRX6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aluminum-activated malate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0831008</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aromatic acid exporter (TC 2.A.85) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRX6"/>
    <dbReference type="KEGG" id="dcr:108192501"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH11521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="57925" checksum="761D49EBF97F3A2E" modified="2024-05-29" version="1">MESLRKYMFGLMVKAWRICGKAWTSIWTVGKEDPRRIIHAFKVGLSLTLVSLLYLMEPLFKGIGSNAMWAVMTVVVVLEFTAGATLYKGLNRGLGTLVAGSLAFLMEFIATEYGKIFGAIFISVAVFVIGSAATYMRFLPDIKKNYEYGVVIFLLTFNLITVSSYRVDNVLKIAYDRFYTIAIGCGICLVMSLLLFPNWSGEELHNSSVSKLEGLAMSIEACVSQYFKDHELEPDISKEESEDHDPIYMGYKAVLDSKSYDATLAKHASWEPRHSRHCYRYPWQQYVKLGAVLRRFGYTVVALHGCLETEIQTPRSVRALFRDPCTQLAGEVSKALTELADSIRKRRQCSPDVISDNLHKALNDLNTALKSQPRLFVGSETNQTSNMLALAAVTARQSVDNHLPSANTESSTLHDWKLSKRVSKKTKEARKVLRPTLSKNAITSLEFSEALPFAAFASLLVETVARLDIVIDEVEELGTKANFKEFYADDEITVTCERFKVETASDLKNNLPEQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XX23</accession>
    <name>A0AAF0XX23_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Peroxidase</fullName>
            <ecNumber evidence="3 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0935384</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 18"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XX23"/>
    <dbReference type="KEGG" id="dcr:108200201"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF28">
        <property type="entry name" value="PEROXIDASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="6 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041779148" description="Peroxidase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="40"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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            <position position="269"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
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    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="50"/>
            <end position="130"/>
        </location>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="83"/>
            <end position="88"/>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="136"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH15838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="341" mass="36702" checksum="E94CA283D80DC8B8" modified="2024-05-29" version="1">MAMHLNIYLFMILMNFSLAFATRNLTNLSNEACVGDVSIGLSFGGYQEICPEAEAIVYSWVDKAVADDPRMAASLLRLHFHDCIVNGCDASVLLDDTPNFVGEKTAAPNANSLRGFELIDAIKSDIESLCPGVVSCADILTIAARDSVILSGGPGWQVETGRKDSRTASKTAANNDIPAPTSDVQTLVSKFQAAGLDLNDVVTLSGAHTMGKARCTTFISRLNGSADISSGIGPDVNLDFISSLQQLCLAADANAALAQLDLVTPSTFDNQYYVNLISGEGLLTSDQVLVTGDEQTRELVRLYAEDQEIFFEEFRRSMIRMGRLQPAGTDGEIRKNCRAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XXF7</accession>
    <name>A0AAF0XXF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0934312</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XXF7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.20.10">
        <property type="entry name" value="Plasmodium vivax P25 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF515">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042039682" description="Protein kinase domain-containing protein" evidence="17">
        <location>
            <begin position="21"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="405"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH14789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="732" mass="82667" checksum="2BFAFBDA9B77DB02" modified="2024-05-29" version="1">MELVLKIILVSSVLIQEVSAQETSVAMPDCPQKCGDVTIHYPFGIGRNCSLDWRIQISCNDNFTPPKPFLNYSNLEVVNISVLEGTIEVKNPVFKDCSNRGLAQRVVFVYKPFRFSSTRNLFIAMGCNNLAVIKNLEGSNLGGCMSFCDQKTLTIDKRDCFGVKCCQAIVPPYFWNFTTSLRTTDDDKNLDDICKYAFIVNKKWFENLTDIYSVQLMKEVPAVLDWNLNLTDECYTLRPTNVSRNMSVCGTYATCHNLKCSCLKGYQGNPYLPHGCRDTDECAPSHKKVCAQNCMNTPGGYRCSCKRGWELDTNARGCTEATNVKKARIILAIASCTILGVVLVLAATWWLCKVMKRRRVRIVKEMNFERNGGLLLRQQVSSIEGNVDTTKLFSSKELDMATDHYNKDRILGQGGQGTVYKGMLADGSIVAVKKSKIDDESKKEQFINEVVLLSKTNHRNIVKLHGCCLETDVPLLVYEFIPNGTLFEYIHDYNQDFPLTWDIRVRIAIEIAGALFYLHSASSLPIYHRDIKSSNILLDKKYKAKLADFGTSKSIAIDQTHLTTRVQGTFGYLDPEYFQSSQYTDKSDVYSFGVVLVELLTGQKPILAPRAHDEERSLATHFILAMKENRIFDILDPRFAKEGDKEEIKLFADIAYRCLNLNGRKRPTMKQVAAELESIDKSHTLLTAEQQYEEIEYPVSHLNGLWDLTSTSMSSGTSNSVKVDIEPLIPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XZE9</accession>
    <name>A0AAF0XZE9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0935262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XZE9"/>
    <dbReference type="KEGG" id="dcr:108201516"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH15719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19795" checksum="80A5FB25073B06DC" modified="2024-05-29" version="1">MTNFQKVIRSLLELLSIPERKSTVYEDLKQLCHPDIVRPHSVTTTAHQDLIRIGVSSRSNAITISEMGKKFAEAAGDNRNALLSDASLNDVPDEEIYNHLQGVCGLGAYNAYTIMIFGLEKMLDHWPNEAEFRLKYNKFNEQLRRCPSSDEAVVASKSWMPFRGLATWIIWTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y114</accession>
    <name>A0AAF0Y114_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0935804</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y114"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF7">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH16254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="646" mass="74998" checksum="7EF4652F0FDA0D6A" modified="2024-05-29" version="1">MKVGLLRWKTMVILVLMLITVLSPIFLYTDTRFSIPSSSSFILDPIPDNTSIHQVSTFAVRPLNVLPQESLTVLKQEPGIINANNSESSRKTRQLTEEDNHEEAKDENPIRQVIEEEKVESKAVDKDEPLEVEKNDPVEQEKVILTTTTTKKEDPKFEQKPAKTSKKVSSREAPKVKPEKQNAPTVPVDARVRHLKDQLIRAKVYLSLASTRNNPHFIRELRLRMKDVQRVIGDSTKDSELPRNAVEKLKAMEETLVKGRQMQDDCSAVVKKLRAIIHSTEEQLRVHKKQALFLTHLTAKTLPKGLHCLPLRLTTEYFTLNTSERQFPNQEKLEDPRLYHYALFSDNVLAAAVVVNSTVSNAKDSSDHVFHVVTDRLNYAAMRMWFLINPPGKATIQVQHVEEFTWLNASYSPVLKQLNSPSMIDYYFKNRRADSDPNMKFRNPKYLSIMNHLRFYMPEIFPKLNKVLFLDDDVVVQKDLTRIWSLDLKGKVIGVVETCGESFHRFDRYLNFSNPLISKNFDPRACGWAFGMNVFDLEEWRRQNITEVYHSWQNLNHDRQLWKLGTLPPGLITFWKRTFPLDRSWHVLGLGYNPNVSQKDIERAAVVHYNGNLKPWLEIGIPKFKTYWTKYINYDQLYLRECNLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AK80</accession>
    <name>A0AAF1AK80_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-adaptin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0104869</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Adaptor protein complexes are heterotetramers composed of two large adaptins (beta-type subunit and alpha-type or delta-type or epsilon-type or gamma-type subunit), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <sequence length="844" mass="93621" checksum="43688C9697A91177" modified="2024-05-29" version="1">MPPQPAAQRSASPSQPSGKGEVSDLKAQLRQLAGSRAPGTDDSKRDLFKKVISYMTVGIDVSSVFSEMVMCSATSDIVLKKMCYLYVGNYAKYNPDLALLTINFLQRDCKDEDPMIRGLALRSLCTLRVPNLVEYLVGPLGSGLKDGSSYVRMVASIGVLKLYHISASTCVDADFPALLKHLMLNDPDAQVVANCLSSLQEIWSLEANTSEEASREREALLSKPIIYYLLNKIKEFSEWSQCIVLELVSKYVPPDSSEIFDIMNLLEDRLQHANGAVVLATIKLFLQLTLSMTDVHQQVYERIKAPLLTQVSSGSPEQSFAVLSHLHLLVMRAPMLFSTDYKHFYCQYNEPFYVKKLKLEMLTAVANESNTYEIVTELCEYAANVDIPMARESIRAVGKIALQQYDVNAIVDRLLQFLEMEKDHVTAETLVLVKDLLRKYPQWSHDCIAVVGNISSKNIQEPKAKAALIWMLGEYAQDMQDAPYVLESLIENWEDEYSAEVRLHLLTSVMKCFFRRPPETQQALGAALAAGIADFHQDVHDRALFYYRLLQYDVSVAEGVVNPQKQAVSVFADIHSSEIKDRIFDEFNSLSVVYQKPTYMFTDKEYRGPFAFSEELGNLSIGVEAPETIVSSQIVDANDKDLLLSTSEKEDNKGLSNNGSAYNAPAYDGSLSLTAPQVQSDLLSLDAPLPSHPPHSSLAIDDLLGLGMSVASTPPTPPPPVLKLNAKAVLDPNTFQQKWRQLPVSVSQDASISPQGVAALTNPQSLLRHMQGHFIQCIASGGQSPSFKFFFFAQKSEDSTIFLVECIINSSTSKAHLKIKADDESSSQAFSTLFQSALSNYGMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKW5</accession>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG83835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="350" mass="37759" checksum="1BC894DC20B92D60" modified="2024-05-29" version="1">MAEKNQFVAGVTVGPSCYLDDQRHARRKQRFTLFNAALDMLSGSPNEKNRKNKKSVSRKDDKINGNNAEGWKKFVGSMRPLHMQATRSSSPSPRHSPISEPALGFPLSATSWGTASKYDSPNSLHESGSTGTMSLGTGTVSLGLSPCASPNNLQGASTGTTSPDLSPCASPNNLQGASTGTMSSNSSPDNLDKSTGTTSPGLSPNNIGTMSNSVSDDDLEEFIGTVSHSVSASNLRNLAIANSRRKHRKSSGDRSKLGRKSRMSPSKSTSNLQDLSLQKMRRYKSALNLHDLDLNEETEAEEDTDDKLLEKCAADVMIDVKADQFIKQFYQQMKSQHKGSKASTQTLTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AM51</accession>
    <name>A0AAF1AM51_DAUCS</name>
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        </recommendedName>
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        <name evidence="6" type="ORF">DCAR_0206994</name>
    </gene>
    <organism evidence="6 7">
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF166">
        <property type="entry name" value="OS10G0104700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="614"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG87763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="779" mass="88614" checksum="1C2036D5B0BA8269" modified="2024-05-29" version="1">MALKKVIAICQSGGNFETGKDDSMSYIGGEAYAIDLDAQSQLSHFKEELAEMFQYNVDSLSVKYFLPGNRKTLITISRDKDLQRMVNFFEESGQVELFILSKEGTSQDVVNAPLISSSRANTEGMNISHEILNIAEADGTTDIEMDTANGLTRSLVVSGTTDDNCRNIVMQWENIITGVDQRFSDFTEFRDALHKYSIAHGFTYKFIKNESQRVAVKCKAEGCPWRIWASMVSGTQLFCIKKMNSTHTCEGAAVKAKHRVARDWVGNIIKEKLKVSPNYKTRDIVGDIKREYGIELNYSQAYRAKGRAREKLHGSYRKAYSQLPLFCEKIVETNPGSVATFTTKEDSSFQRLFVSFYASISGFRQGCRPLIFLDYTPLNSKYKGMLLSATAADGNDAFFPLAFAVVDEESDENWHWFLSHLKDVASTSQRITFVADFQKGLVEALREIFGGGAFHGYCLHHIAEKLNKDLKEPFSYEARRLMVQDLYAAARASKLDAFERCTESMKAISPEAYNWVIRSEPDHWATAFFGGARYGHISSDFGRPFYSWVSDAKELPITQMVDSLRGKLMELFYKRRIDSSQWETKLTLSMEEKLKNEISKAHSLQVTHLHDSTFEVRSESVDIVNIEHWDCSCKSWQITGLPCCHAVALIKLLDRSPYDYCFRYFTAESYRATYSESINPIPNVEGTVMSEPTETAFIVTPPPTKSPSPKVGKRRKQDGTFDIIRRTLQCSKCKGLGHNKRACRLVILKQLHFKIYIKVFIPAKMPNPRTCYKLIRYMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMB7</accession>
    <name>A0AAF1AMB7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">SPARK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0104508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040336">
        <property type="entry name" value="At1g61900-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059003">
        <property type="entry name" value="At1g61900_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33831">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33831:SF5">
        <property type="entry name" value="OS07G0102300 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="At1g61900"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
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            <begin position="85"/>
            <end position="233"/>
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    </feature>
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        <location>
            <begin position="287"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="48932" checksum="BFD9C1A7B3DF609C" modified="2024-05-29" version="1">MRELVVQSLCVYLNLLIVLICKFYLFFGLSGSCCNSLHSASPAILNGNEPLLPVTSPTAAPQPFLPLLAPSPLGPFTNSSVPKLSGQCLLDFGAAQSMMSDASIDCVATFAPYLANVVCCPQVEATLTVLIGQSSKFSKELALNGTSAKHCLSDFQQILVSQGANNSLQQICSIHASNLTEGSCPVKDVDEFESIVDTSNLISSCEKIDLVNECCEQVCQNAISEAAKKLALKAYDLMNIDGHHAVTDQSTRDGDCRNIVLRWLANKHNPSQAKKVLRGLSNCKINKVCPLVFPDVSPVTKGCHDGVSDQRACCNALAGYASRLQKQSFTTNLQALDCAATLGMKLQKENITKNVYELCHISLKDFSLQESGCLLPSMPSDVILDQYSGISFLCDLNDNIPAPWPSTSQLPAQSCNKTIKIPALPAAASGQRSIYSKDVKYFLLIVASVVLTIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMT5</accession>
    <name>A0AAF1AMT5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0208199</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMT5"/>
    <dbReference type="KEGG" id="dcr:108209200"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015215">
        <property type="term" value="F:nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000075">
        <property type="entry name" value="Nicotinamide adenine dinucleotide transporter 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="10"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="113"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="213"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="319" mass="34880" checksum="A4D09DE4E1689A2D" modified="2024-05-29" version="1">MTAGDRRSFRDIFCDAAAGASAGAIAATVVCPLDVIKTRLQVHGLPNASHSNRKGSVITTILQNIIRSEGLKGLYRGLSPTLAALLPNWTVYFAVYGSLKSLLRLHGDDNGQLTIGANMVAASGAGAVTAIATNPLWVVKTRLQTQAMRQGIVPYKSIFSALRRIAHEEGIRGFYSGLLPSLVGISHVAIQFPAYERIKSYLAKRDNTTTTELSTEKVAIASSLSKISASVMTYPHEVIRSRLQEQGQVRNSKQQYKGVIDCVKHVLQKEGLPGFYRGCATNLMRTTPSAIITFTTYEMIKRFLEQTLPFSEKHPEAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMW8</accession>
    <name>A0AAF1AMW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc transporter 6, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208305</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMW8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF26">
        <property type="entry name" value="ZINC TRANSPORTER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="35922" checksum="69CC8E6EFDE7C4BC" modified="2024-05-29" version="1">MTICAYDSGRELWCRDGKAAAHLKLISIFIIFFTSVIGISLPVLFARFFQGKPAYDKAVLLIKCFAAGVILSTSLVHVLPDAFNALADCQVSSHHPWKDFPFSGLVTLVGVLMALLVDLTASSHVDSHSERDVSEYKPIGAAEMKCCGGKVVEREADLESEERMSVAEEEMAVRVSQVLEIGIIFHSVIIGVTMGMSQNKCTIRPLVAALAFHQIFEGMGLGGCIAQAGFSFGTTAYMCLMFSVTTPMGIVLGMLVFFVTGYDDHSSNALIIEGLLGSLSSGILIYMALVDLIALDFFHNKLMNSEIFLKKVSFLALFLGSTFMSILALWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AST7</accession>
    <name>A0AAF1AST7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0310917</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AST7"/>
    <dbReference type="KEGG" id="dcr:108211356"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042182145" description="glucan endo-1,3-beta-D-glucosidase" evidence="18">
        <location>
            <begin position="24"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="19">
        <location>
            <begin position="365"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG91667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="480" mass="53033" checksum="46B753A1369E3638" modified="2024-05-29" version="1">MGLWATSLCVLLFVIFEGFRVECLGVNWGTMATHKLPPEDVVQMLKDNGINKVKLFDAEQSTMSALAGSGIEVMVAIPNDQLDSMTSYKRAEKWVQHNITRYTFNGGVNIKYVAVGNEPFLKSYNNSFLNITFPALQNIQNAINEAGHGDTIKATVPLNADVYGSPEDNPVPSAGRFRQDILGQMTQIVQFLDKNKAPFTVNIYPFLSLYGNDNFPIDYAFFDGATLPIRDNGIDYLNVFDANFDTLVSSLKAAGFGDMPIIIGEVGWPTNGDKNANVRYAQRFYNGLLSRLAANKGTPLRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKYEMDFSGQGQNKFLVPAQNVKYLPQQWCAFNPDAKDLSKLAENVNYACTFADCTALEYGSSCNGLDANANASYAFNAYFQVQNQGDLSCNFQGLASVTTQNISQGECNFTIQIMSSSRSSSAGPSLVVILFLAFLTLIMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AT71</accession>
    <name>A0AAF1AT71_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0311391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </dbReference>
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        <property type="entry name" value="THIOREDOXIN Y1, CHLOROPLASTIC"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="37"/>
            <end position="133"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="140" mass="15909" checksum="7070B4FB9AB7C81A" modified="2024-05-29" version="1">MQADYVYHILFITLLKFFVLHATSYSLNMKVKAKKQSFSTFDEMLATSEKPVLVDFYATWCGPCQMMVPILNEVSASLEDKIMIVKIDTEKYPGIADKYSIQALPTMIIFKDGKPCDRVEGALTASQLIQRIETTLKVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATJ7</accession>
    <name>A0AAF1ATJ7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3 8">peptidylprolyl isomerase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0311146</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
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            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
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        <property type="entry name" value="PPIase_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="60"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="170"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="289"/>
            <end position="381"/>
        </location>
    </feature>
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            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
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            <begin position="481"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="588" mass="65070" checksum="3E0C0EB9262DC278" modified="2024-05-29" version="1">MVIITESNASNLVNEEDEEPGEVIESAPPLKVGEERLLGSSGIRKRLLKRGLAFELPDIGDEVTIKFVGWLLDGTKFSESDGVTYALGSGKMVSGLDRGIVTMKKGERALFTLPPELAYGEGTNGVPPNSVINFQVELISWITVIDVSKDGGIIKRVLVKGEQTGKPSDLDEVLVKYAVMLADGTLLAKTPEEGVEFYLNDGHFCPALSKAIKTMNRGEKVNLAVQPNYAYQLGSMNSDQNSHAIPKNTVLSIDMELLSFKRVISVTTDSKVMKKILKEGQGALTADEGALVTVRYKAMLKDGSIFEKKGYDGEEKLQFITGEEQVITGLDRVAMTMRKGEQAMITIDPEYGFGSVEVRRDLAAVPSYSTLSYEVEMLNFVKEKEPWELIGSERIQAAEMKKNEGNVLFKNGKYQRALKKYDKAATYVGEDGSFGDDSQKLVKSLRVSCWLNNAACCLRLNNYQGAIELCSKVLDTEFSNVKALYRRSQAYIETADYHLAEADIKKALEADPGNREVKAIQLKLKKLQAESNKRDAKLYSNMFSQMTKRLKLENVEDTKKNEVTMAMETENVGVSCGSPDNEMIVDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUH3</accession>
    <name>A0AAF1AUH3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUH3"/>
    <dbReference type="KEGG" id="dcr:108212511"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47169:SF2">
        <property type="entry name" value="OS01G0541250 PROTEIN"/>
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</entry>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG96465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="634" mass="70267" checksum="A348570FE5FCE703" modified="2024-05-29" version="1">MATRQNEKPNNIVYSLLFLVYLFSYSSAISTCPNCGTIEVPYPLSTSNNCGDPEYSLRCEPHTKKLYFDALNGSSYLVLKINASIQRIVLQPSSWLPGTCVTQDMLVSEGLWLNQTLPFNITSSNTIFLLNCSPRLLASPLNCTPSSICHHYLESSGHIDATRALKCSNGPINPCCTFIAGGMPSAYKIRLHNSGCRAFRSILGLDSDKPASQWEEGLEIQWTSPSEPVCKSQIDCSATSKCSPAGNTGVLRCQCKRDYNWNHVLGTCIKKKNYSKVGRNLKISIAVVLFFVIAVVMALVTMKKSGKFSNRAKLVKAREEMLKSSSGCRSARMFSLKDIKKATNGFSKDKILGVGGFGEVYKGELRDGSIVAIKSAKVGNIKSTQQVLNEVAILSQVSHQNLVRLLGCCVEAEQPLMIYEYISNGTLHDHLHGKFSSNFLDWKCRLRIALQTAEALAYLHSAAYTPIYHRDVKSTNILLDGEFNAKVADFGLSRLASPGLSHVSTCAQGTLGYLDPEYYRNYQLTDKSDVYSYGVVLLEILTSQKAIDFSRAEDDVNLAIYASQRENKGAIMEVLDQRLLAEDHSVNFMTSLRLFSDLAFSCLREKKGERPSMKNVVQQLECITEMVTRQESQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AY62</accession>
    <name>A0AAF1AY62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicastrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0416782</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AY62"/>
    <dbReference type="KEGG" id="dcr:108219308"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000075">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041084">
        <property type="entry name" value="Ncstrn_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008710">
        <property type="entry name" value="Nicastrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092:SF0">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18266">
        <property type="entry name" value="Ncstrn_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05450">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041923765" description="Nicastrin" evidence="11">
        <location>
            <begin position="22"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Nicastrin small lobe" evidence="12">
        <location>
            <begin position="46"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG97442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="667" mass="71839" checksum="F98C44BA56042390" modified="2024-05-29" version="1">MDTKLLLSLLCLIASLRSSFSASFQADLKSVPDLEKSMYSIIDGFPCVRLLNLHGEIGCSNPGNGKVVAPVVRLDSAENIVQPSALLVSSDEYDSLLKRISDDSHFARNVAGVLVQSDMQDKLKGLSPDTKFPQAEFSPYDSRTHQWNPNGSGTMWGRYNFPVFLLSQSSTAKLQEVAEKNEKRNKGSATHVAEFDVVMQTTKAGTHNSESCLEEEACLPLGGYSVWSALPPIDTTLSTQSKPIILAVTSMDAASFFRDKSLGADSPISGLISLLAAVDALSRVDGLKDLAKQLVFVVFTGEAWGYLGSRRFLAELDQHSDATSGLSHMLIETVLEVGSVGKGFDDGVKSFFAHATGVSSGTNDTLKALKKAQESLGTESIKISSASSSNPGIPPSSLMAFLNKDSRTSGIVLEEFDTVFDNKLYHSHLDDITNINSSAIIAAATIVARSLYILASDNKELSSSALSAVSVNTSLVEELLGCLLSCEPGLSCDLVKQYISPTSSCPSHYVGVVLEEPSSTPYLGYVGDVPRFVWNFLAEKTSFPSKNDSSACPKDCSNTGESCIKAETDGKGVCVISTTRYVPAYSTRLKFESESWKVLPKNASDPMGVADPVWSESNWAPIQLRVFLVQDSSYDYLILFVGIGLTVLSYIAIVVARSIIAKSMKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYW2</accession>
    <name>A0AAF1AYW2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS4c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0519817</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYW2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000081">
        <property type="entry name" value="30S ribosomal protein S4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1050.10">
        <property type="entry name" value="Ribosomal Protein S4 Delta 41, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003717">
        <property type="entry name" value="PRK05327.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="66"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH00454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="166" mass="18994" checksum="3F17ABDD6B94FE67" modified="2024-05-29" version="1">MSRYRGPRFKKIHRLGALPGLTNKRPRAGNDLRNQSRSGKKISISYSSRRKTKGSTGQVLLQLLEMRLDNILFRLGMATTIPGARQLVNHRHILVNGRIVDIPSCRCKPRDIITARDEQNSRALIINSFNSPSQDEMQKHLTLQPFQYKGLVNQIIFDLNLHILNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1G6</accession>
    <name>A0AAF1B1G6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0521929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1G6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH02540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="129" mass="14630" checksum="45B486BC60B596EE" modified="2024-05-29" version="1">MKHISGSGNKFHPCFCTRQIRSNPYCYKGVPRLQTGFQLMQTTSSIEKILPEVLVPFLVVRDEADKVTDPAVSKLLFETASSSDKTINYGEFPENLDIIFSDVIAWNGQLEKERKIRNDTVETENSSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3K5</accession>
    <name>A0AAF1B3K5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide methionine sulfoxide reductase A5</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0624760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRA family specifically reduces the MetSO S-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3K5"/>
    <dbReference type="KEGG" id="dcr:108224954"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000004">
        <property type="entry name" value="Peptide methionine sulfoxide reductase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF3">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="11">
        <location>
            <begin position="55"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH05344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="29318" checksum="8989A4C10523211D" modified="2024-05-29" version="1">MKQSDRKLCIYYNHHHYTLLVVYMMWLLSMNIVVLGIRIPDRIQKDPKDEVVVKTAVFGLGSFWRSESVFGCLDGVVRTAVGYAGGSKTNPEYRSLGDHAECVKIEYDPRVINFRQLLEVFWSNHDSRQVFGQGPDVGNQYRSIIFTNGTEESRLATLSKEREQNRLKSSIVTTQIQPLGPFYPAESDHQKFELKRNHFLLQLIGNLPEEELQSSSLAAKLNSYAAELCPWRTQKRLDAKINDIVRKGWPILQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3U1</accession>
    <name>A0AAF1B3U1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0522367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="Probable ubiquitin-conjugating enzyme E2 23"/>
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        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBC_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="467"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
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        <location>
            <begin position="717"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="717"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="745" mass="82410" checksum="7DA163A33445EFAC" modified="2024-05-29" version="1">MESPAIGRSYIAAKAKKRVFPGGSSMDPDVIEIPPPTPINRSSKSKTLKLKEVVDQEIIDVDMDENSCNVMLTEGIGTNYKGKGAITNNSLGIDGDGGQSSKKNSAPGSHSVVNLEGFACDLSFLDDDYVDMYPDGAHVFDDNQMYEDEYEILQSHFDNIDIPPGVEAPVSWFPGPAQNKINVGATKNPALSNPKPQPSPGVILPEMEASHSLWSLDPWGRMPTTNTLGMETPVQGVSHPHKAGLSSSWSTIENAQSKKNIASSKYFQHKAQSSGGWGFVNKSTPFGLASSHGPHKPPGSLNQQYIEAKAAFRGDNILRKKTKHIARNPYIPNASEASPVTSSWYKHDNSMYVMVPPNYPTHYNPFLSEHMSFDGVAYDPFLQDSFTIQTNATDLGVPLGSSSGSQKDATFLDCPSNSSQQNVTDKHVNRDEILMKLHQFKKFDVVQDPSDHHYVHNGSSSKQPSKSWAKKIQEEWKILEKDLPDTIFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDVLFPDNYPHVPPHVYYHSRGLRLNPNLYNNGKVCLSLLNTWSGSQKEMWIPNLSTMLQVLVSIQGLILNAKPYFNEPAYDRLSGTPHGEKSSQHYNESTFILSLKTMVFTLKKQPKHFEDFVVGHFFKCALDVLVACKAYMDGAQVGCLVKGGVQDVDEGDKSCSQNFKATLGAYLKPLVDAFTQIGVKDCEQFIPPSQTGNSQTGFRQSGHRHMSPALKTPNYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4Z6</accession>
    <name>A0AAF1B4Z6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0624250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISP family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH04838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Putative pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAP_sf"/>
    </dbReference>
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        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
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        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042268866" description="SCP domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="25"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH04838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="161" mass="17608" checksum="5E00F913B5F2CB4A" modified="2024-05-29" version="1">MENKYLYLLTFLTLATLSHHSHAQNSNQDFLNAHNTARSQVGVGPMVWDAVVAQYALNYTNSRIGDCGLVHSGGPYGENLAKGSGSFTGTAAVNLWVAEKADYNYATNTCASGKVCGHYTQVVWRNSVRLGCARVLCSNGVWWFITCSYDPPGNYIGQKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5D6</accession>
    <name>A0AAF1B5D6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5D6"/>
    <dbReference type="KEGG" id="dcr:108227320"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000494">
        <property type="entry name" value="F-box/LRR-repeat protein 10 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF86">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="649" mass="71987" checksum="057949F29D57A791" modified="2024-05-29" version="1">MSSEGSDPPQPAPPSSPSETLDLLPIALLGTILTKLDIPSICSAASTCKSFNSSASTILSFLPHFHLLDVAPSIGLLRPLLPPNNPHLQTLHLDCARLDDSSLDCLLRPSLRHVSLHNCADFSGSLLSQLGIRCKLLRSLYLSSVADKRWRSMCVSDLEQLLTGCTQLETLSLMFDVSIFLRPNFARVWPLASAKITCLEIGYISSVMVTELLSPILGPQQSPSHMQCSILPNIHKLWLAVDYITDTMIGTISKSLVSLTHLDLRDAPIIEPSVTIDLTNSGLQLINPHGKLKHLSLIRSQEFVATYFKRVNDLGILLMADRCESMESICLGGFCRVTDTGFKTILHSCTSLYKFKVTYGSQLTDLVFHDIDATSLALTHVSLRWCNLLTNLSFVRLASNRNLSVLDLRDCRNLGDSSLKAIGTLHKLKTLLLDGSDISCVGLSYLKESVMHSIVSLSVRGCKRLTDKCISILFDGSSSRELRELDLSNLPNLSDATVLLLAKSRVQLVELRMRQCPLIGDTSVMALASMQADDDTWRGSSLQLLDLFNCGGITPISFRWLKKPYFPRLRWLGVTGLVNRELVDALARSRPFLRVSYCGEELGTNQWDNSNDMYMHDYEEVDELEQWLLDDDNEIADEEMVDAADDVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6F2</accession>
    <name>A0AAF1B6F2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0727390</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6F2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF20">
        <property type="entry name" value="CYTOCHROME P450 CYP749A22-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
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        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH07955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="58956" checksum="D675356AADAED30B" modified="2024-05-29" version="1">MGLIAYLIIFVSGFMTLYVLLAVVRFAGKVWWNPLRVQYKMKLQGITGPSYKFLYGNTKEILVMRRSSMEKPMDDLSHHIFPRILPHVHSWMNNHGANFLNWYGPQAQLVVTEVELVKEILNNKDNSFPKIELEGYAKKLLGDGLSSSTGEKWLKLRKLSNHVFHAENLKNMIPTMVTSVGMMLERWKEHENREIEVFEEFRILTSEVISKTAFGSSYLEGEDMFRMLMKLTLIVSRNLHVVRFPLISKIIKTKDDKESEKLEQGIHDCISKMINNREKESSGVMESNGSGDFLGSLLEANNNRDASKRLLMQDMVDECKTFYFAGHETTTSLLGWTILLLSTNKQWQEKARNEVVELFGQANPTSDGIPKLKNMNMIIEESLRLYPPVPVIKRKVERQVQLGNLKLPPHLELYISPLALHHDPQIWGEDVHIFKPDRFAKGVANATKNTAAAFLPFGFGPRTCVGLNFAIVEAKIALAMILQRYAFTLSPTYVHSPIQTFMVRPQHGVQIILLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8B2</accession>
    <name>A0AAF1B8B2_DAUCS</name>
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            <fullName evidence="3">Adenylyl cyclase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0727848</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH08408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41532" checksum="096825A22825BC54" modified="2024-05-29" version="1">MLKTASSVGRLARVFKHSRPALLFSDPLHLTAGDETPLSGSSDSPWIPQRRHLSSGMFHLYNTVKELYDKILKSVNEQRTAPPNAWLWSLIENCANVEDIKLLFDVLENLRRFRLSNLRIHENFNENLCREVTKACVRVGAIDFGKKALWKHNVYGLSPSVGSAHHILMHAKENNDANLMVEVMKLLKRNDVQLQAGTADIVFSICYNTDKWDLISKYSKRFVRAGVKLRETSFDVWMEFSAQRGDIESLWYIEKLRSDTMKRHSLSTGFSFAKGCLLERKPEIAAATIHVLSQTLSEAKKPGIIVELQKLVSDWTLKVIKCQKEEDRKDLAAALQADIPAMVNGLVELGLDVNVSTEDLLAKDLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B9Q0</accession>
    <name>A0AAF1B9Q0_DAUCS</name>
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        <alternativeName>
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    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0727892</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 13">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 12">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4 10 13"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B9Q0"/>
    <dbReference type="KEGG" id="dcr:108196047"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11314">
        <property type="entry name" value="AmyAc_arch_bac_plant_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012850">
        <property type="entry name" value="A-amylase_bs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013775">
        <property type="entry name" value="A-amylase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07821">
        <property type="entry name" value="Alpha-amyl_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001028">
        <property type="entry name" value="Alph-amls_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00810">
        <property type="entry name" value="Alpha-amyl_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041785941" description="Alpha-amylase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="14">
        <location>
            <begin position="27"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal beta-sheet" evidence="15">
        <location>
            <begin position="365"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001028-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH08451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="431" mass="48175" checksum="BB4B3B974C29661C" modified="2024-05-29" version="1">MPTFATFPSLLCLFFLILHTFAAPSPTLLFQGFNWDSCNKQGGWYNYLINSVSHLAAAGVTHVWLPPPSQSVAPQGYLPGRLYDLNVSAYGNEGELKALVKAFNDKGIKCIADIVINHRCAEKKDSRGIWCIFEGGTADDRLDWGPSYICRDDTAYSNGKGNLDTGLDFSGAPDIDHLNLRVQRELSDWMNWLKTEIGFDGWRFDFVRGYSPSITKIYMQNTSPEFAVGEYWDSLSYGPDGKPNYNQDNHRNALAQWVQTGGGVVTAFDFTTKGILQAAVQGELWRMKDSNGNPPGFVGISPGNAVTFIDNHDTFSQKMWPFPSDKVMQGYAYILTHPGIPSIFYDHFFDWGLKDAISKITAIRIRNAISETSSVQIVASDADLYVAKIDDKIYVKIGSRYDVGDLVPPNYKVSTSGTDYCVWEKIKNQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAS2</accession>
    <name>A0AAF1BAS2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0729033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAS2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507:SF21">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE CHAIN 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9853" checksum="AEC529362E29E4B3" modified="2024-05-29" version="1">MICIYNIIYSHLYKRLYSATKFILYTAGGSVFLLMGPTLNFATLVNQSYPVALEIILYIGFFIAFAVKSPIIPLHTWLPDGVLHIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAU7</accession>
    <name>A0AAF1BAU7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0729109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAU7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000079">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="200"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH09651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="354" mass="39979" checksum="B55DF97C033D3E46" modified="2024-05-29" version="1">MENHGDHFHEAQYTDQNGVLQTTKIPVVQELARKGITNLPQRFITSPQTLSATTQVEVPTIDLLKLRVESDSSRYRELAKLAGAAKEWGMFLVANHGVHDSVLDSVKDVVMGFFGLSFEEKKKSVGSFMSVDNMGYGRNFVRSENQPLDWIDRMTMVASPVDDENLRVWPKKPHNFREVMVKYAEETQVIFSDLLQAMAEALSLDKNAFLQQFEPKRSEPDLAIGITPHSDASGLTLLIQFGANDGLQVLKDNKWLSPIWPQDMLLVNIGDLMEIMSNGKVTSSWHRATTSTNYERFSVAVFYNPPPDAEIEPMRGENSGEGMYKKVNVREYVKHYYKISPTVGKEAILYAKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDT2</accession>
    <name>A0AAF1BDT2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0935253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDT2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH15710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="148" mass="16793" checksum="CAC0F55A2A360237" modified="2024-05-29" version="1">MRTSPISLEDAFDFYCKGISSAGPIWDQILGYWKESQKNPNKVLFMRYEEMKDEPYLQLRRLANFLGIPFSPEEENSGLIDQIISICSFKSMTKVDVNKTGTVVDGFNNSSFLRKGVVGDWKNCLTADMASRLDQITEEKFRGSGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WSG5</accession>
    <name>A0A161WSG5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_010369</name>
        <name evidence="11" type="ORF">DCAR_0311800</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WSG5"/>
    <dbReference type="STRING" id="79200.A0A161WSG5"/>
    <dbReference type="Gramene" id="KZN01615">
        <property type="protein sequence ID" value="KZN01615"/>
        <property type="gene ID" value="DCAR_010369"/>
    </dbReference>
    <dbReference type="OMA" id="HECKEEP"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04480">
        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04481">
        <property type="entry name" value="RPA1_DBD_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="7">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="9">
        <location>
            <begin position="128"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="8">
        <location>
            <begin position="282"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN01615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG92530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49455" checksum="62C14C3EB5739557" modified="2016-07-06" version="1">MAIKNILIHVRATRIWESYTIDKNNFQKKLLNTKVIFVDEEQSQIMVQIWNNQKEDYFPMLKEGSFYAISEFRVVPILKAYRAVATELALGFDHNTKVVPKEDTDRIPYFRFNPTKFEDMPSLLWDTKNFIEVVGLLEEYGDQETASNRAKKLDILLLDSRNNNMIVTLWEDKAAQFQEGLQQSNEGPIFVIVTGLLVKKYSGQNIVLSSGDATKTYFNIDCTPITELKHTMVAAAEKNNTNVSPPNKKVFVSTAENALKTVKIQNILDIELTSTSEMMRFICEASIISISKYDGWYYNSCPKCPKSIRIDSNTFYCDSCKKEIDGYTQRYKVIIGVQDDSGKTTFTLLNNDAEQLIGIPVQGIIANLGQDKLTADIPPVLNNIIGKKCAFEVKVTSFNRDGRAGYTIGCLMELPGSSSGIKGGRSTDDEGPSKKIRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XXT9</accession>
    <name>A0A161XXT9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_010258</name>
        <name evidence="6" type="ORF">DCAR_0311632</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XXT9"/>
    <dbReference type="STRING" id="79200.A0A161XXT9"/>
    <dbReference type="Gramene" id="KZN01477">
        <property type="protein sequence ID" value="KZN01477"/>
        <property type="gene ID" value="DCAR_010258"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214835"/>
    <dbReference type="OMA" id="ETRAGCS"/>
    <dbReference type="OrthoDB" id="420046at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012272189" description="Aminotransferase class V domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class V" evidence="4">
        <location>
            <begin position="82"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN01477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="628" mass="70092" checksum="3A6B39B13A1F777F" modified="2016-07-06" version="1">MAFFRLIIILVIALAHPIANHQEKSLWSSSRKLVTEFKSFGSLENGLPETNSTEEKLSWLRSQVIGANVAFETPFGERLLTYADHTATGRSLRHIEDYILHNVLPFYGNSHTSDSYVGQQTTKMVDEASRYIKKCLGGGEDDALIFCGSGTTAAIKRLQEVMGISIPSIMRERVLAELESRERWVVFVGPCEHHSNILSWRQSLAQVVEIGLNKDGLLDTNDLVLQLQHYSSTKRPMLGSFSACSNVDGTYTDTRALAYLLHHYGAFACFDFAASGPYVEIKMRSGEMDGYDALFLSPHKFVGGPGSPGILMLNKALYRLKSSPPSTCGGGTVKFVNCFSETDTLYIQDIEEREDAGTPPIIQKIRAASAFWIKEYIGYKSISNQEQNYTKRALERLLRNPNISVLGNTNRSRQAIISFLVQTTTRPSSNELINPDKSKQNETDNKRSKPLHGRFVAKLLNDLFGVQARGGCACAAPYGHRLLNLNEPASSAIRSAIQKGKGGARPGWTRISFPYYMSNEEFEFLLEALEFTATYGQRFLPMYNFNWETGAWTLRKEVSSHQLPIKGICEPDMTKEEQISAVTKGVEISGVYASYLSTAKQIACLLEEFPSQRPVPDDIDTDILTFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y6S0</accession>
    <name>A0A161Y6S0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0728850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y6S0"/>
    <dbReference type="Gramene" id="KZM88049">
        <property type="protein sequence ID" value="KZM88049"/>
        <property type="gene ID" value="DCAR_025124"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196367"/>
    <dbReference type="OMA" id="HIANTHN"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd02972">
        <property type="entry name" value="DsbA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="1">
        <location>
            <begin position="58"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="26763" checksum="389F9FE98D1EB0C7" modified="2016-07-06" version="1">MATLKHSNAQIPIVNRTNIIIVVLLLLLAEIDEAQVTIPDKYDGFVYSLPKGPDFISIEAFLDPVCSDSRDSWLPLKQLVHHYPSSIFLIVHPFPLPYHDNAFITSRALHVVDKINSSATFGLLEKFFENQERFYNAQTFNWSRASVVDLVAKFAAKATGLSNKSAIQAGFEDSTTDQATRISFKYGCSRGVFGAPVFFVNGFVLPNQGTPVDYETWRSIIDPLLDKKGITRQDLVQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZIP8</accession>
    <name>A0A161ZIP8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_023916</name>
        <name evidence="3" type="ORF">DCAR_0727431</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZIP8"/>
    <dbReference type="Gramene" id="KZM86782">
        <property type="protein sequence ID" value="KZM86782"/>
        <property type="gene ID" value="DCAR_023916"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM86782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="226" mass="26473" checksum="FA8D022DE7FBBBBB" modified="2016-07-06" version="1">MERKGRAARGRPTGNFSKSRPITASAKRGSKRRNQSSKQWKEIKYIKLDELKGIQAEVEKYKDLIDEEIPKALKVNGSNIIRYAYNILERVKRCVDSKTLEEVSKGDAEALEVALQKLIDCSWEGPVVRFADRKMQERSEKREESHRKIILQCERWVDENVLKMVEDGCEEGLRMATNQTHYKSLRNMEYGKSFYEALMGKKEEPIKADDKEEDWTVVRRRRRNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZM59</accession>
    <name>A0A161ZM59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Lipoxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_024394</name>
        <name evidence="22" type="ORF">DCAR_0728224</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZM59"/>
    <dbReference type="STRING" id="79200.A0A161ZM59"/>
    <dbReference type="Gramene" id="KZM87260">
        <property type="protein sequence ID" value="KZM87260"/>
        <property type="gene ID" value="DCAR_024394"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195869"/>
    <dbReference type="OMA" id="INPCALQ"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01751">
        <property type="entry name" value="PLAT_LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000005">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000001">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042057">
        <property type="entry name" value="Lipoxy_PLAT/LH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 17" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PLAT" evidence="19">
        <location>
            <begin position="84"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="20">
        <location>
            <begin position="214"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KZM87260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH08776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="906" mass="102572" checksum="EF2F0ED31C684BCB" modified="2016-07-06" version="1">MLKPQIHNLSSSKTLLQCHKPFFSGNLGAKPAFPNIKRARSSSSGNKNKNISTSVSHAARSSSSIKSVANYTETTTEVLATVVVQFTVSGFLSNLGWNPLDDISDLFGKSIQLELVAAHLDPSTGLEKKTIKGYAHRAGNVDADDVKYECKFKVPNDFGEIGGVKVENEHHNEMFFKTIQLDGFPNGSILVNCESWVDSHRDNTDNRFFFTNKSYLPSDTPEGLKKYRENELMKKRGDGTGERKSKDRIYDYDVYNDLGDPDKDAKLARPILGGKEFPYPRRCRTGRPRSKKDPLSEKRGSSVYVPRDEEFAEVKSLSFSAKTVYSVIHAVIPSIENVSIDAELGFPYFTAIDTLFNEGIDLPEVPKSTLINLLPRLLKTVTDQGKNLILFETPEFLDRDKFKWMKDEEFGRQTLAGLNPCCLQLVKEWPLKSQLDPKEYGPPESAITTKTVEEVIRGFMTVDEAIKKKKLFIIDYHDLLLPYVKKVREIKGTTLYGSRALFFLTPIGTLKPVAIELVRPPMDGKPQWKEAYAPGIDATSGWLWKLAKAHFLAHDSGFHQLISHWLRTHCATEPYIIATNRQLSEAHPIYRLLHPYFRYTMEINALARQALINADGIIESSFAPRKYSIEFSSVAYDKLWRFDHQALPEDLISRGMAEKDPRAPHGLKLTIEDYPYANDGLILWDTIKQWVTDYVNHYYPKPGQIESDEELQAWWEEIRTVGHGDKKDEPWWPNLKTPKDLVQIITTIIWVTSGHHAAVNFGQYDFAAYMPNRPTIARVKMPSEDPTDEEWKTFERRPEDALLSCFPTQLQASKIMAILDVLSNHSIDEEYIGEAPEPSWAEDPAIKSAFEVFAGKLKELEGIVDARNNDKKLRNRTGAGVVPYELLKPYSEPGVTAKGVPNSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZS57</accession>
    <name>A0A161ZS57_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_022053</name>
        <name evidence="2" type="ORF">DCAR_0624112</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZS57"/>
    <dbReference type="STRING" id="79200.A0A161ZS57"/>
    <dbReference type="Gramene" id="KZM90582">
        <property type="protein sequence ID" value="KZM90582"/>
        <property type="gene ID" value="DCAR_022053"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF57">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM90582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="201" mass="22553" checksum="D18134E83DB7C5D6" modified="2016-07-06" version="1">MEVMVPLAARDFSFDSSNSVPLSPRGFSYYFDQNGGGKYRDSEAFTFSIRGDLNSASADELFDNGKIRSGEPIRKKIIASFGRNNKVCSEHSTVSKENAKELSSAGSNVGWLEKKWKLKYMFPKKLKEEDMKNSSAKKKKNERGVVALSAHERHYKVKRAAAEEMRRRTFLPYKQNLMIGCVDFAAAGVSDPSRASIKVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A3I5</accession>
    <name>A0A162A3I5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_017932</name>
        <name evidence="5" type="ORF">DCAR_0520537</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A3I5"/>
    <dbReference type="Gramene" id="KZM94690">
        <property type="protein sequence ID" value="KZM94690"/>
        <property type="gene ID" value="DCAR_017932"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223741"/>
    <dbReference type="OMA" id="ARRFINM"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000856">
        <property type="entry name" value="AFG1-like ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF29">
        <property type="entry name" value="AFG1-LIKE ATPASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM94690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH01156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57467" checksum="E911F216FA118ABC" modified="2016-07-06" version="1">MRRFVACGRHLRSAFRRQSDIIYCGIGRRSYFVDTNRKALCKQSYNVDQCLHGHLSFVISRTLYADVSRNTDEELSSSWPSGPLVEYERRIAAGELVDGDTCQIGTLKELQRLYDELVKSADACRLDRYAASEKAARSRWLWSRFMPQSSYSPVKGLYLYGGVGTGKTMLMDLFFEQLPSNWRKKRIHFHDFMLTVHSRLQKHRGVADPLEVVAGEISDESILLCLDEFMVTDVADALILNRLFGHLFNNGVILVATSNRAPDNLYERGLQRDLFLPFIATLKERCVAHEIGSSTDYRKMTSAEQGFYFIEKYESDVLKQKFEQLIDSHSAGPQEVEVVMGRRLLVPLGANGCAYFPFEELCDRPLGAADYFGLFKIFHTLVLDGIPIFGLHNRTAAYRFVTLVDVMYENKARLMCSAEGTPFDLFERIVTISDAQQMAPRTSSRSRKNDEYDLCVDNELGFAKERTISRLTEMNSSEYLEQHAELLAEKWLPQSDNKEAIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A9X9</accession>
    <name>A0A162A9X9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_014446</name>
        <name evidence="10" type="ORF">DCAR_0416484</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A9X9"/>
    <dbReference type="STRING" id="79200.A0A162A9X9"/>
    <dbReference type="Gramene" id="KZM98192">
        <property type="protein sequence ID" value="KZM98192"/>
        <property type="gene ID" value="DCAR_014446"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218880"/>
    <dbReference type="OrthoDB" id="1930739at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF473">
        <property type="entry name" value="OS05G0437100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="160"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM98192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="363" mass="39024" checksum="E58FB6477BF491EC" modified="2016-07-06" version="1">MNLPPKEYLAAEGSTMYVDHQFSQSQNSGDHLQYSYRPQQVTTSGGSGMMWFGGGGDGGGIGSGGVISYGVNSSSPSYSSGSSSSGQKRTRSDQESSVTTQFPGQQLPKLQRGISQDSSSLSSVKEEPDGIAPAAADATPPPAAEIDQSAQPGASYERRKYRGVRQRPWGKWAAEIRDPHKAARVWLGTFETAEAAARAYDEAALRFRGSRAKLNFPENVRMIPPPLAPPPQPRMANSSSSAFHQPAPFLSQQFEASDTLKDYFEYSRLLQSSGDLQLHQQPNLLHQMLNVPTAPTQSFGGSFSSQSSVTMLNSPFGQPFPNQQIAGLFQRPAAGTRGEGSSQDFPATSSRMPGQYFPPSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AAZ2</accession>
    <name>A0A162AAZ2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_014468</name>
        <name evidence="7" type="ORF">DCAR_0416463</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AAZ2"/>
    <dbReference type="STRING" id="79200.A0A162AAZ2"/>
    <dbReference type="Gramene" id="KZM98170">
        <property type="protein sequence ID" value="KZM98170"/>
        <property type="gene ID" value="DCAR_014468"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216487"/>
    <dbReference type="OMA" id="LCIFARH"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000051">
        <property type="entry name" value="N-alpha-acetyltransferase MAK3 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="15"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM98170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG97124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="24036" checksum="DA3DB4463BD1E9D7" modified="2016-07-06" version="1">MDEERVKEGEEECEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPNLSFLAFHKGKCIGTVVCKMGEHRNTFRGYIAMLVVLKPYRGKGIATELVTRSIKVMMESGCEEVTLEAEVTNKGALALYGRLGFIRAKRLFRYYLNGVDAFRLKLLFPRPELSSGHLIVENEDMPHEESQPELSSGHLIVENEDMPHEESQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B3V9</accession>
    <name>A0A162B3V9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_002319</name>
        <name evidence="11" type="ORF">DCAR_0102405</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B3V9"/>
    <dbReference type="STRING" id="79200.A0A162B3V9"/>
    <dbReference type="Gramene" id="KZN09663">
        <property type="protein sequence ID" value="KZN09663"/>
        <property type="gene ID" value="DCAR_002319"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205258"/>
    <dbReference type="OMA" id="PWITSGC"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000600">
        <property type="entry name" value="Phosphoenolpyruvate carboxylase kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="9"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN09663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG83230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="275" mass="31198" checksum="0E5F0B1514EE42B2" modified="2016-07-06" version="1">MSEALKRDYTVCEEIGRGRFGVVSRCYSAVSGESFAVKSVEKRLLADDSIDRQCLYNEAKIMQVLAPHPNVIQVYDVYEDEDWLHLVIELCNSPDLFCRVSERVFDESEAKSVMVPLLEAIAHCHRLGVVHRDLKPDNVLFDEWNQLKLADFGSAECVRENEMLTGVVGTPYYVAPEVLAGRNYNEKVDVWSAGVILYVMLTGVAPFYGNSATEIFEAVLRANLRFPVRYFHSVSPAVKDLLRRMLCKDASRRLSAEQVLGHPWMQGNREMSIPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B7U9</accession>
    <name>A0A162B7U9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_003759</name>
        <name evidence="2" type="ORF">DCAR_0103988</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF23">
        <property type="entry name" value="OS12G0134500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH09019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="211" mass="24421" checksum="8EC644A365718B49" modified="2016-07-06" version="1">MTSNEEAYSQFGRRSNQPENEDYGETQVTSNNDGISINRLMDANLPRRRISRRHRVVVVRRREAIRQRRDAYIQQFRRANHMNSEVHSSGIESQSRFDSVYPEVFMTTIEYAETLESLAEDDDVELKATPTAKSFIDSLKVKKVECEKDIPFCVVCQDMMNVGDFLKELPCMHNYHVDCIQSWLATRNTCPVCRFELPTSDLVLGSVEDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WJD2</accession>
    <name>A0A164WJD2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_020787</name>
        <name evidence="13" type="ORF">DCAR_0625560</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH06137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WJD2"/>
    <dbReference type="Gramene" id="KZM91848">
        <property type="protein sequence ID" value="KZM91848"/>
        <property type="gene ID" value="DCAR_020787"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226816"/>
    <dbReference type="OMA" id="KHAMQTQ"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF591">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="57"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM91848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH06137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="27833" checksum="2D50D4BB55584FC7" modified="2016-07-06" version="1">MEWNNGNNQIRPSFLSHQSTSFNFLYNSHYPETETQAGAHGGMGDVMEDVNRVQQHGASHEKKKRMTSEQLEYLESRFQEEMKLDPDRKIKLARELALQPRQVAVWFQNRRARWKTKQLEHLYLSLKHQLDLVSMEKHKLQEEVVALRAILKQEVTRKQVWGGCNNPATTTDMTSAEDTVESTSVFPSSMHVKTTRVRSCTENNNMNVTANYQGTYVFEADEYNNSANWGAAGTAVMPCYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X8N1</accession>
    <name>A0A164X8N1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alanine transaminase</fullName>
            <ecNumber evidence="9">2.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0518431</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Photosynthesis; C4 acid pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X8N1"/>
    <dbReference type="Gramene" id="KZM92845">
        <property type="protein sequence ID" value="KZM92845"/>
        <property type="gene ID" value="DCAR_016090"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222962"/>
    <dbReference type="OMA" id="AYMARTM"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000140">
        <property type="entry name" value="alanine aminotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="155"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG99083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="531" mass="58208" checksum="DE6645E28E2E06A6" modified="2016-07-06" version="1">MRRFVANRLVKTAAAASLFSSNKTTTTTTSSTPLLSRFLTSSPPPNPIMPSHSITLDFINPKVIECEYAVRGEIVNLAQKLQQELVDKPGSHPFDEILYCNIGNPQSLNQQPITFFREVLALCDHPAILDKSETQGLFSADSIERAWQILDQIPGRATGAYSHSQGIKGLRDTIAAGIEARDGFPADPNDLFLTDGASPAVHMMMQLLISSKNDGILCPIPQYPLYSASIALHGGTLVPYYLDEATGWGLEISELKRQLEDAKSKGVTVKALVVINPGNPTGQVLAEENQRDIVEFCKAEGLVLLADEVYQENVYVSEKKFHSFKKVSRSMGYGDKDIPLVSFQSVSKGYHGECGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMSPPKVGDESYESYTAEKDGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPEKAIKAAESAKTAPDAFYARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVERLTEFHKGFMNEFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YPT2</accession>
    <name>A0A164YPT2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_018060</name>
        <name evidence="4" type="ORF">DCAR_0520676</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YPT2"/>
    <dbReference type="Gramene" id="KZM94818">
        <property type="protein sequence ID" value="KZM94818"/>
        <property type="gene ID" value="DCAR_018060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM94818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH01294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="111" mass="13136" checksum="EA4B9C76EFEB576C" modified="2016-07-06" version="1">MDSNTQMNLLQLTNENASMEERIKMLQAENSILEHKIKLMEIQQTHDEAVVKVLKDHIEERRAFNRLLRDDSNFSPSEMERHEKIREEIINEHEAKKRAKVSPNVDEQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZWD8</accession>
    <name>A0A164ZWD8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_019758</name>
        <name evidence="11" type="ORF">DCAR_0522603</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group II-b family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZWD8"/>
    <dbReference type="Gramene" id="KZM96516">
        <property type="protein sequence ID" value="KZM96516"/>
        <property type="gene ID" value="DCAR_019758"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222123"/>
    <dbReference type="OMA" id="HQENDQH"/>
    <dbReference type="OrthoDB" id="2020995at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000002">
        <property type="entry name" value="probable WRKY transcription factor 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF50">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="279"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="503"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="507"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM96516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="541" mass="58779" checksum="7C542878E5A64E27" modified="2016-07-06" version="1">MAKPCGLTFLHHNNNLSPQSYIINSFPTQQESHWKAEARPMDLSPPRGSFQFQLSTAHDHDQDRPDEMDFFARKRDDHDRDQSKVTDNLASEADDHRDLSDFNVNTGLHLVTANTCSDHSIVDDGLSPDSDDKKAKNELGALQADLERTNIENHRLKDLLSQVTNNYNTLQMHLVTVMQQQSHKSADYEPEEFINLGLGGQRSAEADAEPSLSLPEGGEHIRSPANNMDHSSKEDDVDPGSQNAWMGSHKVSRLNPSNSVDQATEATIRKARVSVRARSEAPMITDGCQWRKYGQKIAKGNPCPRAYYRCTMATGCPVRKQVQRCADDRSILITTYEGNHNHPLPPAAMAMASSTSSAARMLLSGSMPSADGTINSNVLARSFLPCSNNMATISASAPFPTVTLDLTQNPNPLDCQRPQNQFQLPFHNPQQNVASSAALLPQIFSHALHNQSRFSGLQMSQDLVPPHQAGSLADTVNALTADPNFTAALAAAISSVIGGGSNPTGSPTNNVNVTQTPTNNGSLTTSNNNSNGNIRVTNSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WNG1</accession>
    <name>A0A165WNG1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0415708</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WNG1"/>
    <dbReference type="Gramene" id="KZM97537">
        <property type="protein sequence ID" value="KZM97537"/>
        <property type="gene ID" value="DCAR_015101"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217583"/>
    <dbReference type="OMA" id="HKHGLVQ"/>
    <dbReference type="OrthoDB" id="1056333at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010547">
        <property type="entry name" value="TOM20_imprt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409:SF3">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06552">
        <property type="entry name" value="TOM20_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="203" mass="22397" checksum="87317CFD537F54FB" modified="2016-07-06" version="1">MDMQTDFDRILFFEHARKAAEASYSKDPLDSENLTRWGGALLELSQYQNVVEAKKMLDDAMSKFDEALMVDPKKHDALWCMGNANTSYAFLTPDTEEAKVYFDKAADYFQQAVDQDGGNELYLKSLEVAAKAPELHSEVHKQGGFAQQAALGTGPSTSTSAKVGNAKKSSDLKYDIFGWVILVVAIVAWTGFAKSNMPSPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WZV1</accession>
    <name>A0A165WZV1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416005</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WZV1"/>
    <dbReference type="Gramene" id="KZM97803">
        <property type="protein sequence ID" value="KZM97803"/>
        <property type="gene ID" value="DCAR_014835"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216471"/>
    <dbReference type="OMA" id="TASRRCK"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="20"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG96669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="152" mass="16008" checksum="A990CA62729F06EC" modified="2016-07-06" version="1">MEGGGKLKKGAAGRKGDGPRKKSVTRSVKAGLQFPVGRIGRYLKKGRYAKRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKSRIIPRHLLLAIRNDEELGKLLAGVTIAHGGVLPNINPVLLPKKSEKAAAAKEPTKSPSKAAKSPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AS88</accession>
    <name>A0A166AS88_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        </source>
    </reference>
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        <text evidence="10">Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.</text>
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    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="WOG94490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="106" mass="12101" checksum="CC0307A4751974AF" modified="2016-07-06" version="1">MGKRCGEWREDISRHSNDATAVLPISMPGFENPERSSQMRSEVTNINHTGPIRTPPSISDQMRRYHSHHVQKTYMHAHMNKAKHIRVGDEATPLPTNTLYVGLLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
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    <gene>
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        <name evidence="7" type="ORF">DCAR_004826</name>
        <name evidence="8" type="ORF">DCAR_0205380</name>
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    <organism evidence="7">
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="7">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF123">
        <property type="entry name" value="9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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            <end position="42"/>
        </location>
    </feature>
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        <location>
            <position position="294"/>
        </location>
        <ligand>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </reference>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="87"/>
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    </feature>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG87643.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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</entry>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EXJ7"/>
    <dbReference type="Gramene" id="KZN07097">
        <property type="protein sequence ID" value="KZN07097"/>
        <property type="gene ID" value="DCAR_007934"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207775"/>
    <dbReference type="OMA" id="DKYKKMT"/>
    <dbReference type="OrthoDB" id="779403at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000018">
        <property type="entry name" value="agamous-like MADS-box protein AGL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF387">
        <property type="entry name" value="AGAMOUS-LIKE MADS-BOX PROTEIN AGL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG89733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="28855" checksum="26D5A5FB44976154" modified="2016-07-06" version="1">MTRKKVKLAFISNDATRKATYKKRKKGLMKKVEELTTLCGIDACAIIYSPYDEQPDVWPDIAGVERVVEKFKSMPEMQQSRKMLNQESFTRQRIAKTNEQLKKQLKENREKEMTEVMSQCLTGQLGLHNLMLPDLNDLGFLVDQKLQEINKKMNEMALQEATQQQQVVTPEEAPQEQQVVPTTVSDNVGVLAGGVVEEQRLDAGGNTDVDAYGLVPQWFNNDAVVSDNVTADYQDLGFMTPFGDENNPVAWANFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F5U7</accession>
    <name>A0A166F5U7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008213</name>
        <name evidence="3" type="ORF">DCAR_0209288</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F5U7"/>
    <dbReference type="Gramene" id="KZN07376">
        <property type="protein sequence ID" value="KZN07376"/>
        <property type="gene ID" value="DCAR_008213"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007873116" evidence="1">
        <location>
            <begin position="29"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN07376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="171" mass="17272" checksum="890A0EDF2455CC08" modified="2016-07-06" version="1">MTYYNLAKLVILVSTLVFLSGSLPQGHAQVQPQLVTGLTGLLNITRIQFTTVQLACTPTGNPPSTGGGVAGVAGALFGGSCNGATGSLAGVFTNASGFAQGILTLAEGIVIDPSRGMPCFINTRLPVTGTTCTVLPPTGLLQAVFQLISVVTNPFGGLLAVATTGPWVIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F9T4</accession>
    <name>A0A166F9T4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pectate lyase superfamily protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_008357</name>
        <name evidence="8" type="ORF">DCAR_0209447</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F9T4"/>
    <dbReference type="STRING" id="79200.A0A166F9T4"/>
    <dbReference type="Gramene" id="KZN07520">
        <property type="protein sequence ID" value="KZN07520"/>
        <property type="gene ID" value="DCAR_008357"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207850"/>
    <dbReference type="OMA" id="SNANMEV"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF66">
        <property type="entry name" value="OS06G0106800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007873188" description="Pectate lyase superfamily protein domain-containing protein" evidence="6">
        <location>
            <begin position="32"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="478"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN07520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="58516" checksum="92980F7417C608BE" modified="2016-07-06" version="1">MGSKNHRIHQVRNVFIVIASVILLSANNVDGKKDIAPKSQGAEYAAINCRAHSASLTEFGGVGDGKTLNTKAFQDAIKHLSQFESQGGSELIVPPGKWLTSSFNLTSHFTLFLHKDAVLLASEVETDWEVIAPLPSYGVGRDTTEGRYISLIFGTNLTDVVITGENGTIDGQGRIWWDKFKKNEIKHTRPYLIEIMFSTDIQISNITLVNSPNWNVHPIYSSNIIIQGITILSDLHSKNTDGINPDSCSNIRIEDCYIESGDDCVAVKSGWDQYGIAFGMPTKQLIVRRLTCISPTSAMIALGSEMSGGIEDVRVEDITAINTESAVRIKTAPGRGAYVKDIYVRGMTLNTMNWVFWITGNYGQHPDNKTDLNAIPVVENINYQNVVAKNVSIAARLDGIPGHQFKGICISNVSIEMAPSATPPIWNCTDEVAGMTTSVTPPPCPQLAAQGPPACKFVTDKLPIENVKLKMCPVPTGGTAPGAQPPTPSASAPTAFRGVASTLPGGKAPSAAPEAPSVASVENSPLPAPGLNAPLAAPGLNAPLGAPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FMG5</accession>
    <name>A0A166FMG5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE homolog, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100396</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the plastid-encoded plastid RNA polymerase (PEP) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FMG5"/>
    <dbReference type="Gramene" id="KZN07948">
        <property type="protein sequence ID" value="KZN07948"/>
        <property type="gene ID" value="DCAR_000617"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204768"/>
    <dbReference type="OMA" id="FLHYDTR"/>
    <dbReference type="OrthoDB" id="533138at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.20:FF:000006">
        <property type="entry name" value="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.10">
        <property type="entry name" value="MurE/MurF, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001126">
        <property type="entry name" value="PRK00139.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23135">
        <property type="entry name" value="MUR LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23135:SF4">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-L-ALANYL-D-GLUTAMATE--2,6-DIAMINOPIMELATE LIGASE MURE HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="MurE/MurF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase N-terminal catalytic" evidence="5">
        <location>
            <begin position="261"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase central" evidence="7">
        <location>
            <begin position="350"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="6">
        <location>
            <begin position="578"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00064883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="771" mass="84986" checksum="5CC1D5FCB2C72C89" modified="2016-07-06" version="1">MTLHTIFTLSPPLPISLKSSFPPKFLKTHCPNFPIFRIKSPLSLTPFAIGPDGKFYPTPSDNDPPEAAEDATHGVNKYTQISRQASRARKIADEEYKKDQSIFLNAIADVEDAPDNPNLAEENDSGDDLFGEIDKAIALKRKEFVKQGLLKPNPKKEKGKAGEVVDELEPEEADDLEEIDELQGLAGNVEDSELESSDLEVSDDDLRKNDLSDNESFDVDFNSFGSGKARIVDPKFRMTLAELLDESKVVPVSVYGNLEIEITGIQHDSRMVESGDLFVCCVGKKNDGHLFLSEADKRGAVAVVASKEIDIEDTLGCKALVLVEDTSAVLAALSASFFRYPSKKMSVIGITGTNGKTTTSYLIKGMYEAMGVRTGMLSSVEYYIHGDNKLESPHTAPDTVLVQKLMAKMEHNGTEALVMEASSHGLASGRCDEIDFDIAVFTNLTKGHSDFDGSEEEYRDAKAKLFSRMVDPERHRKIVNIDDPHAAFFISQGNPEVPVVTFAMENKNADIHPLKYELSLFETQVLVNTPQGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEEQAFGVIVDYANTPDGLSRLLDLVRELGPRRVITVIGCCGESERGKRPLMTKISTEKSDVTLLTSDNPRNEDPLDILDDMLSGVGMTMQDYLKHGENDYYPPLPNGNRLFLHDIRRVAVRAAVAMGEEGDVVVVAGKGHETYDIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWRLPESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FYR8</accession>
    <name>A0A166FYR8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Staygreen protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_000893</name>
        <name evidence="9" type="ORF">DCAR_0100848</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the staygreen family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FYR8"/>
    <dbReference type="STRING" id="79200.A0A166FYR8"/>
    <dbReference type="Gramene" id="KZN08347">
        <property type="protein sequence ID" value="KZN08347"/>
        <property type="gene ID" value="DCAR_000893"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205944"/>
    <dbReference type="OMA" id="VGEWPHR"/>
    <dbReference type="OrthoDB" id="2012322at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024438">
        <property type="entry name" value="Staygreen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31750:SF4">
        <property type="entry name" value="LP06106P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31750">
        <property type="entry name" value="PROTEIN STAY-GREEN 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12638">
        <property type="entry name" value="Staygreen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Staygreen protein" evidence="7">
        <location>
            <begin position="54"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN08347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG81697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="272" mass="30872" checksum="5C45434A13192F33" modified="2016-07-06" version="1">MATLTANSLSLHIPPKFKPNITQKSQSSVYYFTRRPKKYSSLFPVARLFGPAIFEASKLKVLFLGVDEKKHPGNLPRTYTLTHSDITSKLTLAISQTINNSQIQGWYNKLQRDEVVAEWKKVKGKMSLHVHCHISGGHFLLDLFARLRFFIFSKELPVVLRAFVHGDGNLLNNYPELQEAMVWVYFHSNLPEFNRVECWGPLIEAAAPSSGFRVRANQNEESPSSNWNLPQPCEEACSCCFPPMSSIPWPRQLSNPVEQSSDEAIQSLPQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J5Q5</accession>
    <name>A0A166J5Q5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0104850</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J5Q5"/>
    <dbReference type="Gramene" id="KZN11824">
        <property type="protein sequence ID" value="KZN11824"/>
        <property type="gene ID" value="DCAR_004480"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211886"/>
    <dbReference type="OMA" id="ACIVEEP"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG85659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="566" mass="61930" checksum="ECC1B7C42DD61939" modified="2016-07-06" version="1">MEENEPLLESNKQKSYGGFRTLACIIANESLEKAAAFGLSANLILYLENEYHMGLVTGTNIINLWGAAGNFLPVPGAFLADSGVGRYPMIAVGSVFGLLGMILVWLTTVIPEARPPICDILTAECSSSTAPQVIFLCFSLGLTAIGAGGIRSASMAFGADQFVKRYNQEESLAALESYFGWYYVASAVAVIISLTFVAYIQEHSGWQVGLGVPAVLMLLGVLLFFSASSFYIRSQDKSSLFTSFSQVIVASYKKRRFTITPDINNVYYHKKESALVVPSDKLRFLNKACIVEEPERHLTADGDIQDPWSLCTVDQVEELKAILKVIPMWSTGVILSVTLSQGSLQLVQALSMDRHITPGFEIPAASLGVFTFISAISSVVLYDRVIVPLASRIMEKPFHLTSKTKMGTGIFLSILSMLVLAYIEYVRRGLATQQGLSEPVVNMSVFWLILPNCLIGIAEAINAVGQCEFFYSEFPKSMSSIGSTLRGLTFTVGGLVATAMLNIVDQVTRRGGKASWISSNINQGHYDYYFLMIAGMCVVNMLYYLVCSWAYGPCELVAVKDSQKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WE42</accession>
    <name>A0A169WE42_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_012555</name>
        <name evidence="8" type="ORF">DCAR_0313646</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WE42"/>
    <dbReference type="STRING" id="79200.A0A169WE42"/>
    <dbReference type="Gramene" id="KZN03799">
        <property type="protein sequence ID" value="KZN03799"/>
        <property type="gene ID" value="DCAR_012555"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211876"/>
    <dbReference type="OrthoDB" id="730183at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF4">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="10"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN03799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG94352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="290" mass="33181" checksum="DF90F2B01D2525EB" modified="2016-07-06" version="1">MGLRDIGAALPPGFRFYPSDEELVCHYLHKKIAHEDVSKGTLVDIDLHTCEPWQLPEVAKLNSTEWYFFSFRDRKYATGYRTNRATTSGYWKATGKDRAVVDPGTNCVVGMRKTLVFYKNRAPNGIKTGWIMHEFRLENPLIPPKEDWVLCRVFYKSKTESNIELSQQNVYDAEARAGGDTSPNAAQIMLPSCNIYHNQTFSSESPAPHDHQNPSNHHTISPLNPNLLLLSHHQFHTDHLNEMIMINSKCEIIDNYGFLMDTSFEDMHPGDGTSNMEMKFEDNHSSIFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YHS1</accession>
    <name>A0A175YHS1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0935606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YHS1"/>
    <dbReference type="Gramene" id="KZM83073">
        <property type="protein sequence ID" value="KZM83073"/>
        <property type="gene ID" value="DCAR_030642"/>
    </dbReference>
    <dbReference type="OMA" id="CENVYND"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35986">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35986:SF1">
        <property type="entry name" value="OS10G0430800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH16057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="136" mass="15809" checksum="3E72D4EFB404700F" modified="2016-09-07" version="1">MKQHISKHFYLEKVFDDLSTDRPKQVWRYRNFFGDDASSSQKTTQSGSYSENTNVEPKPISNESSTDDDTMADPFDWIVHTPVPERVEDNEKAASCTMSHRRHKRSHKRSHRRLISRREASTDMRHALADKGMASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKV6</accession>
    <name>A0A175YKV6_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0830461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH10984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKV6"/>
    <dbReference type="Gramene" id="KZM83758">
        <property type="protein sequence ID" value="KZM83758"/>
        <property type="gene ID" value="DCAR_028820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34355">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34355:SF1">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH10984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="119" mass="13133" checksum="48C69A8B28BC8FD2" modified="2016-09-07" version="1">MSLTLTLSSDTTVKQTTTLNKPTIYTNKNRFTTGSRTTNPKTHNAFPAEFLRRIAEKMAKTLSLVTIKRSSRKVSSSATLARSRSCVDTTLDTHRAEAVSDCIEFLNSSSVLRKCNSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMV5</accession>
    <name>A0A175YMV5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_027891</name>
        <name evidence="2" type="ORF">DCAR_0831561</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YMV5"/>
    <dbReference type="Gramene" id="KZM84687">
        <property type="protein sequence ID" value="KZM84687"/>
        <property type="gene ID" value="DCAR_027891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="entry name" value="OS02G0162600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35101:SF12">
        <property type="entry name" value="OS02G0162600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM84687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="79" mass="8866" checksum="6FF71B12A482E83B" modified="2016-09-07" version="1">MATSLMARYVSEVAPPLFVNSIRRHCMSKVLDTIHEDEKELSSLGDSFSFYVKRSSQERSLQLSSSAFAPVSNKINQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPF8</accession>
    <name>A0A175YPF8_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPF8"/>
    <dbReference type="Gramene" id="KZM84998">
        <property type="protein sequence ID" value="KZM84998"/>
        <property type="gene ID" value="DCAR_027580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH12420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="247" mass="28506" checksum="E3A205BF192EB72B" modified="2016-09-07" version="1">MNELQNSPPTKKRKICTSMSPLILLDVDIILKICSLLQEDGFIDLFFFIQVWFPFQTSETLATLLNNLDWSRVHEVVEPFKNLECRVFNNFVKHSAEIGVKGAMCYVSCKNLIKAKNPQHHLQILKSISAHDSMSFLAYHIFHTLYHPSTLRENAVILHAKCATDSEFRAKVEKNCTTLKGRYRKYNCNRYYRPDIFPQNGVCSSFVSGEDHNMDVFGLGCSYKEIVNSSCSECMILMMNFKIFRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YRQ3</accession>
    <name>A0A175YRQ3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0832539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YRQ3"/>
    <dbReference type="Gramene" id="KZM85542">
        <property type="protein sequence ID" value="KZM85542"/>
        <property type="gene ID" value="DCAR_027036"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </evidence>
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</entry>
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                <person name="Cheng S."/>
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                <person name="Macko-Podgorni A."/>
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</entry>
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            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG83390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="90349" checksum="9EA175815AA2CD53" modified="2024-05-29" version="1">METEKLDQNGDCSMDLSDDQLLNTESSDACGVFDEPELVPRVGDEFQIEIPALVTGPEYYYYLKSPVDAENRGHVPSDFGLGLSVPVTWNKNVNKVMKKGEQDFSGHSTESVEETVDGYQGSSNFLVPGVCADVWNDIEEESFLLGLYIFGKEFSQLKRFVGSKKMEDILFLYYGKFYRSVQFTRWTKITKRRKVKTKKCAYGQRMFSGLGLRELLSRILPHVSDERKKELKEVSTKFAEVCVSLEDYVSTLKDIVGLKTFIEAVAIGKGKKDLTSMANGLPRPKVPVNKPYSSLTHSEIVNILTGGDRLSKAKSNNLFWEAVWPRLLERGWHSEQPKNQAYVPGANNSLVFLTPGVKKFSKSRLKRGNHYYDSVTDILSKVGSEPELLKLDTEKDEGKKEDENGWVEMNNEEEKLPEKRHCYLQPRTPSRDVNIIKYTVVDTSLANGKFYRELSALPKDVFKMDSLSEESDNGTSEVSTNESDCANTMFVNQETKSTRGDKILSERGETLNSSSDKPVQIRGPYSLKVEEKGKPNKLYGDKQTRKPVRSHLSQKLKRNDLDNLASTTKQLRTMLACSNEEIGSGRSTLPLVTKLEEDAHGCCTDNHDPNVKALEVVGSSQDRLSFTISPKGSSPQSTEDAHNDDSHGPENPQSRPLIDLNLPQQPVEFEDGIFMTDSTIMQNDITSKQRDDLCAAKTSADISLPEQPVNINSRRQSTRNRPPTARALEALVNGYLTAATRRKDSHEASRARPHKRSRIEVAATDDVTTNDAVMVGESGSHTIDDGNVNMIDKLQILSNESGSHVSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5V9</accession>
    <name>A0AAF0W5V9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0102754</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5V9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF118">
        <property type="entry name" value="BAHD ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG83577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="50978" checksum="1721EC0F32BC5394" modified="2024-05-29" version="1">MAFRGLIRSSIFRRQLHVISRSSSNIRPASPTPSKLKQYNIPLHDRMIPDFYIPMVFFYPVNSNHSSKAVPDNVSKSDLLKKSLSETLSKYYPFAGRLCSGSYVDCNDQGVHFVDAQIGCQLSEVLEKAPVKEEEEGLGHLFPPRTIWNQLSDLYPGMIMHVQVNHFTCGGIAVAVSLCHRLGDALTFCSFLRYWASLSFNSGDHQKSLHLCPRFVYELLPPSYDSDSIPIVSYPDKNWTTKAVVFPNRKLAKLKAAVENEDKVDGVLEDQKYTRNELLTALIYRCIVASVARTNSGTHGGSALLRSVNVRPMLDPPLPETSVGNFVIPNYILTSTESETKYRTLVGRMREEKKQLNGIKSLEGHEVETKLNELSKKNYRFYAISSICNFPLYDVMDFGWGKPINATLVDTPLVDNITLMDTANDGIRAMIGLGEQDMKFFLSHKELLTYASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBJ4</accession>
    <name>A0AAF0WBJ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Preprotein translocase subunit SCY2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0205245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBJ4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG86049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="63582" checksum="A2DB6FD2B88BBB75" modified="2024-05-29" version="1">MEATLLSSRHSHPQFFPPLHSGKSLVFKSTSVLCRPRYAKTPQFSLRRTNSELSRKDLGLSKGLHCLKMNWNLSLCINDQLRGDYLRVKAASADSLNHELLVPNTDDNSVDDGENLIISYPKPPRYKNRYLNFVHMGTLFNNAAESFFKSEICRRLFVTALLLVMSRVGYFIHLPGFDRKLMPEDYLRFVSGSVDELGDFAPELKLSFFQLGISPQIVASIVMQVLCHFVPSLVKLRKEGLDGQKKIKSYIWWISLCFGTVEGLILACYSLPYSIYAETSRVKHVMVTTLLLVSGAMTMRQGSSLIICVGILSGYKETLHKLLTQVSGSTLSWWPYALAVLVIFTVMTMYAVVVTEGYRKIKLQYYGFKLASSSRQGSPITEVEPYIPFNINPSGMQPILTTTYLLADPSILAGLLGSPVWEYIKQMLNPETSTGAGPWVYYTIYSFFVFVFNIFDIANMPKEVSDYLNKMGARIPKIKPGKATIEYLTKVQAFTSFWGGLLLATLATTSSILDHYLRSNNLGFSISFTSVLIIVGSIIELRRSYQAYNVMPSLSKALKDMVYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ71</accession>
    <name>A0AAF0WJ71_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0208316</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054292">
        <property type="entry name" value="DUF7028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059153">
        <property type="entry name" value="NSD_PHD-1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF12">
        <property type="entry name" value="GB|AAC80581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22970">
        <property type="entry name" value="DUF7028"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23011">
        <property type="entry name" value="PHD-1st_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1343"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="932"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="973"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="10">
        <location>
            <begin position="1072"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1379" mass="157628" checksum="8E149023AAAD0848" modified="2024-05-29" version="1">MAFDPEDEDEQRRREAMTTVNRRKLLEHQKVEVRSCTDEGFLGSWHSGTVIATESSARWVQYDHLLLDEYSSTKLVERIEVPSIVDGIEINNKSMSNYRGWIRPLSPPCYFSIWFLHYGQCVDVYYSDAWWEGVILDHQDGSAERNVFFPDIGDEMAAHKDNLRISQDWDEQTELWKPRGNWIFLELIEEIEKDWPILVSVKQIWYDVRARSGFDNLKEWTSCLTDIWRELLLEVIVDNIKLFLKDFFPTLSSSSSGNTLHDCGHLMELSGPILDAVLKNKECFVDSLAVVPGSNGFLNRSEFLHVDEKGEYRQPCDEINRDKLATVTTFDRLGKMPLSSSVKTIDEEIACASSRTMAIAPSMTGEVSKVNSCTNHEESFNASLEVSKITKNNVWHPAGPNIVSDPDYCPEAVDEYCRLFKSGNKRYLKVQNNVWKHLLFMGWKIEYRNNERINGMQQLRYIEPGSPEKYFVSLVDVCEYLKAGASKELCSFTDLKIPKEKAQDNWVPFCLPEPEFYEDAVTEYINISKCGRQPSEEHILNVLKHLSFLGWKVEYTKETWRYRFIMPKDGGSEKEFRSLFEVCQYMETSSSDIVSMFPQDVQNCLSYAADATLFPTEHSQERKSVLIGPSPDEELIFEPEYCPQAVMNYHTHVTKEKGDHSKWKTGSMLKDMQLKVKKHLFAVGWKLYHYRTKKKLRYRYISPSRKMYDSLRSACKGYIDEEIHVGKLAEVQLPREARYSPSASEEVRKEIHCENKKKRKSSSLHLASLSSNSVTKENKSKKIQAIKSKDNINRPVSVSDRVLRSSKRAREVVDASSSHRNPCTILSWLMDNQVVLPSEKVQYRIGKDCKMMAEGRVTRDGIKCNCCQETFTLHNFEVHANSTCHQPSANIFLENGRSLLECQLQLRRDLNTRGSNRGRLEVKGKPNDNKTDSICSVCHSGGNLLLCDGCPSTFHTRCLGLKGVPTGNWFCPSCCCKICGQSKNSGNIEVFTNSSVINCDQCARQYHISCLRMKGHVLESYPKKYWFCNFKCKQISVGLQTLLGKPVQLGRDHMTWTLLKYIKPDKHYHNQSDIVDSTEIYSKLNIALGVMHECFEPLNEPRTRRDLVEDVIFNRRSKLQRLNFHGFYTVLLEKNDELITTAILRVHGEKVAEIPLVATRSHYRRRGMCHVLMDELEKVLVKLGVERLVLPAIPSMLNTWISSFGFSRMSPSERLELLDYTFLSFKGTTMCQKLLLMTPRIKSNPPSLVKQHSDVISGDDIEGSSGGSEILQTERVDDNGAVGQTCINLGGGVGGEDKSCSDYWVSQPTGFEYVPCQNGEMGLQSSGKGRILKPVAAESPKELVIGMYVLHLLILIYLLNFCCVTRTLQLCHLCCQTDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNY8</accession>
    <name>A0AAF0WNY8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="9">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0311969</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="9"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNY8"/>
    <dbReference type="KEGG" id="dcr:108213312"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF20">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038186">
        <property type="entry name" value="ITPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="110"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF418">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
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        <location>
            <begin position="296"/>
            <end position="330"/>
        </location>
    </feature>
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        <location>
            <begin position="397"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94293.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="65273" checksum="B37D3D4433629186" modified="2024-05-29" version="1">MIRFLNPNHIFIRFFHKQVQETPLSPLITPTILNSLITKSFNYHHATQIHSKIITTNLISGSFVFNNLLNLYAKCGHLDQSLTLFENTQDEVKDVVTYTSVITHLSKCSEGYKALIFFKKMIGACVLANEFTFSAVLPVCGQVGVVFNGEQVHSLICKHGVAMCLFVGSALVNMYGKCGDMGLAKKVFDEMPKRNLVSWDSMISRFLGNKMYVDAVGVFKEVLGGEFVPGEVTFSSVLSACGNMGSLDFGRQVHGVVVKYGLVSLAYVKNSLMDMYIKCGLHEDAYGLFQIIEERDAVTWNVIVMGFVQKGNFEEAWNLFGAMRRKGISPDDVMFSTVLHAAASIAAFDQGSLVHNQVIKTGFGHDLCTVRSLIKMYAKCGSLTLANRVFVECESKNMVTWTTMISSFQQHGCAHQAIELFKNMLEEGIKPSYITFVSVLSACSHTGRVDEGFYYFNAMSEQHNINPGHEHYACMVDLLSRAGRLGEAKNFVDSMPIKPGASVWGALLGACNRFGNLEMGVEVAERLFVIEPHNPGNYVLLSNIYTCKGRLKEASEVRRLMRLNQVSKKTGYSWIDSNNQDARLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUU4</accession>
    <name>A0AAF0WUU4_DAUCS</name>
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            <fullName evidence="4">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">DCAR_0313851</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUU4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
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        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="162"/>
            <end position="330"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG94555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="48560" checksum="82245B8EC557C92C" modified="2024-05-29" version="1">MVGVSDIPCVGKVVDRISDATVNALFRGLRYMFCYKALVDELNSEISKVNIEEVRVSRKAAAERDNGKIIYDHVLEWQKEVKEIQESTNEFSPSWRCIQSLPIPNPISRFRQGRQAVKKAKKVNELLDSGVKLMENEIAYHPAVVNEPKPETAYQRFQSREGAYGKLWDALVTEGSAPILGLYGMPGVGKTRMMEQIWSEAKEKKIFKKVVREDVGSETLDVIKVQNQIAGYLECNFESQDNVDRRASQLKHSLLNKGKILLILDDVWREIPFDVIGIPCEDEISSMGSKILLTSREKDACLRNKCKDPVKITILTEEEAWDLFKNTVGACQIESLQDEFLARRVSLKCSGLPLVIRAVGKALLFASHNSWKDALAQLEKGSVEKIAGIDPHVYACFKLSIDRLEEDAKSCLFLCSLFPEDADIYVRKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUZ2</accession>
    <name>A0AAF0WUZ2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0414425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUZ2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="40" mass="5019" checksum="9CCED34F95319BB5" modified="2024-05-29" version="1">MHVHHHYNYDNPYIRKNTFTKFASINYFLDLQKQHIKHAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WW03</accession>
    <name>A0AAF0WW03_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416469</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WW03"/>
    <dbReference type="KEGG" id="dcr:108215973"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018088">
        <property type="entry name" value="Chalcone/stilbene_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF14">
        <property type="entry name" value="CHALCONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00441">
        <property type="entry name" value="CHALCONE_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="7">
        <location>
            <begin position="242"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG97130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="43571" checksum="A53405683EFB77A6" modified="2024-05-29" version="1">MSNHNAEIEEIRKRQRAQGPANILAIGTATPSNCVYQADYPDYYFRITNSEHMSDLKLKFKRMCEKSMIRKRYMYITEEYLKENPNVCAYEAPSLDARQDLVVVEVPRLGKEAAAKAIKEWGHPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNAGARVLVVCSEITAVTFRGPSDSHLDSLVGQALFGDGAAAVIVGSDPDLSVERPLFQLISAAQTILPDSDGAIDGHLREVGLTFHLLKDVPGLISKNIEKSLKEAFGPIGISDWNSLFWIAHPGGPAILDQVELKLGLKEEKMRATRQVLSDYGNMSSACVLFILDEMRKKSIEEGKATTGDGLDWGVLFGFGPGLTVETVVLHSVPATITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4E1</accession>
    <name>A0AAF0X4E1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0520611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="3">
        <location>
            <begin position="348"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="5">
        <location>
            <begin position="471"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="4">
        <location>
            <begin position="1169"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH01230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1360" mass="158203" checksum="E57048EDAA53D31D" modified="2024-05-29" version="1">MHVHSMSWNQVTMDRFNAFLGECSRIRVSHALNYNACNNLFVNNQVNDNMYLLQVLARHDQFSFFAFNIFRAFFYNCVFEQSAEEMFFRSGEDDHYSPYSWPPTVDRIVDEEGRDFEITVTRKWSDTARDCRVQGIYLIVVDKYENRTHCWIKSNSAFAIADKLEEGHAYIISRFSVEAINLKFRCFEGDVHVLLHKQTIITPLQSNVSGIPQDIFKFTNLSKLADYADDDSCLVDVVGIVENLRPYQTIDGPRDEKHFYKEFYLSDLIFWDGFAMHFEKKYNEGNSNPRIITISSCKILKNQYTGELTIRNVQPTRLFVNFKTGKEDSIMKRIQILVMMNTMQFIKHSPITDLNTSTIKWTVRARAQAIWKGISRDTKEFRGINILLVDDSRSRIHGFIAAKLAPLFENELVEGDIYEISNFIVQDYTGLEFHRCVRFDKHIFFAQYTKMEKCTSPGLTIPKLVVDLFSLKDLQPMEEDKRFLCDVCGVLKNPQEFRDYINDNDEPKQQKKFTLTDGCSEIGVTLFDDMAKSFEEELQKSGVGTKIIILSSVKVGKFQGDVNLTNYPASRFYINADHHAVKKMEKRATTTSFFHPEEVVVKQLESVPMKEMCIADIKKLDYETKVTARVIVKKIREDLNWFFYICTKCNSELDYVDGRYKCPQCNRFFPWPQKRFRVFVLCSDKTGVLPLVFGDREIRRLTGKMVFDVELDLTEEEDGKFPPLFNNFINKEYVLTIDVSEENLKLNSEVYQISDVQMDVEEKLSDLENFDNLSEEAEDDLTIQDIELKTQSSRTAAKKKKLITTEDLPKSDEEAKRIKAKHDPLRKLKNIKIKKEKIVIREKKKSNSNSQPRSSEKKKGRKLIDVISESDDEEMTLNTYQLKNKKQAHTTGKSRLEFYDIGKEYQALVMDGKESTFSYWMISSTFQNFRMHAFVPGKVIENNETKLSDGNLCIISNFTIKNYENTEKFRVVNHDKQIILTTYTHIEKVEEDDGFIQKNMFDFYDLGQLDDIADKNIFLTDVVGIIENDTPIADLVNRFGKKQKQVKFNIVDGRSSVNVCFWDNMAEKFNETIQNVEEHPTIIIISSAKVTSWQPQKMNAKQYELANVTATTFYINYQDESVAALRRMYARGLFGKYNFVNYVKPKYEEITVKEVKKLQMHDAEKQIICKVQIKEVLETGIWYRYHCTSCYKSVEMKNGNFKCYRCADRNVPEPDLRWEITVIAEDKTGEIHIVLFDREIRSIFNFAVTDFDDEVLQSGRVPTILKALEKQNFYIRLHIKEANILGKLNSYYANGVSVCQSAITNDTEETTTPLSAVANTLTQESGPSYHLEDFSDPNIKTTEVDKRPQRKKKLTKKFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5D9</accession>
    <name>A0AAF0X5D9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0521484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5D9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="105" mass="12157" checksum="6D030E912C4D5583" modified="2024-05-29" version="1">MSFCYCGRVAADRVAWTDANAGRRFKNCIGGTNGCRYFQWVDGPLCSRAQVVIPGLLRRLRDMEANYENQVLEAQKKENKKWRLRMCVICLVWLVLYVFSGSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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            <end position="264"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="264" mass="29059" checksum="38E4961006198B83" modified="2024-05-29" version="1">MRTVALGLSLNSLSVNFNNTPSSTPPCRFPSHLSRSVLFIGICKASQVAELFPTLSPEIVVREARLEDCWEVAETHCSSFFPEYSFPLDFVLRIDRLLAMVMGFSVPNGCQRTCLVAVTGSSDEDAFCIGSEDLKIGGFDGKFSLNKGYVTGILTVDTVADFLPRKGPLRNRRTGIAYVSNVAVRDRYRRKGIAKKLIIKAEALAKSWGCRAVALHCDLNNPGAVDLYKGQGFKSIKVPEGAKWPQPKTAPDVQFNFMMKILNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="6">Protein DETOXIFICATION</fullName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0522480</name>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF01554">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9B9</accession>
    <name>A0AAF0X9B9_DAUCS</name>
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            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="6">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
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        <name evidence="10" type="ORF">DCAR_0623228</name>
    </gene>
    <organism evidence="10 11">
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9B9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="7">
        <location>
            <begin position="146"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="9">
        <location>
            <begin position="382"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH03828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="487" mass="54219" checksum="F3E4E6A4E6F9EEBF" modified="2024-05-29" version="1">MGAENNFDMDDGTLEIGMEYRTVSGVAGPLVILDKVKGPKFQEIVNIRLGDGTTRRGQVLEVDGEKAVVQVFEGTSGIDNKYTTVQFTGEVLKTPVSLDMLGRIFNGSGKPIDNGPPILPEAYLDISGSSINPSERTYPEEMIQTGISTIDVMNSIARGQKIPLFSAAGLPHNEIAAQICRQAGLVKRLEKTENLLEDGEDDNFAIVFAAMGVNMETAQFFKRDFEENGSMERVTLFLNLANDPTIERIITPRIALTTAEYLAYECGKHVLVILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLATIYERAGRIEGRKGSITQIPILTMPNDDITHPTPDLTGYITEGQIYIDRQLQNRQIYPPINVLPSLSRLMKSAIGEGMTRRDHSDVSNQLYANYAIGKDVQAMKAVVGEEALSSEDLLYLEFLDKFERKFVAQGAYDTRNIFQSLDLAWTLLRIFPRELLHRIPAKTLDQFYSRDVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9X3</accession>
    <name>A0AAF0X9X3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0623815</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9X3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000036">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000026">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000011">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000011">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024750">
        <property type="entry name" value="Ca_ATPase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF448">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12515">
        <property type="entry name" value="CaATP_NAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.47" evidence="8"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCM8"/>
    <dbReference type="KEGG" id="dcr:108227744"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07783">
        <property type="entry name" value="ASKHA_NBD_FGGY_SePSK_AtXK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000220">
        <property type="entry name" value="D-ribulose kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000180">
        <property type="entry name" value="D-ribulose kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF80">
        <property type="entry name" value="D-RIBULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="10">
        <location>
            <begin position="66"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="11">
        <location>
            <begin position="330"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH03841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="484" mass="53379" checksum="8E292C591978827E" modified="2024-05-29" version="1">MVALLDHFTSSHPLIRPQLGFPNYNSKKWIQGAGRLWIVNNSRYIRMRVVGSKAPKDVNLVDGKRLYLGMDFGTSGARYTLIDKDGIIRAEQKREYPKYMSKDVIDWVHSWKLTLFQLLEDIPNEFRALIASISIDGTSSTTMIIDSTTGEPLCRPFLYNESCPDAVSIVTSIAPVNHTVCSSTSTLCKLVSWWNSYGSSKDSAVLLHQADWLLWLLHGKLGVSDYNNALKVGYDPELESYPPWLLSQAYSYVLPSVQAPGTFIGLVKEDVRTRFGLPKDCAICTGTTDSIAAFLAARATQTGKSVTSLGSTLAIKLLSTNRIDDARYGVYSHRLDDKWLVGGASNTGGAVLKQIFTDEQLKNLSEQIDPMKVSPLDYYPLQTVGERFPVADPKLAPRLTPRPKSDVDYLHGILESIARIEGEGYKLLTDLGATPVEEVLTAGGGAQNETWTRIRERILGLPVSRAQQTEAAYGAALLALKGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIR2</accession>
    <name>A0AAF0XIR2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0728387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIR2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08838">
        <property type="entry name" value="ArfGap_AGFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044820">
        <property type="entry name" value="AGD14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46085:SF3">
        <property type="entry name" value="ARF GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46085">
        <property type="entry name" value="ARFGAP/RECO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="11"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="665"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="683"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH08936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="697" mass="74107" checksum="E5343F8998EE8C34" modified="2024-05-29" version="1">MSNRKEDEKNERIIRSLLKLPDNRRCINCKSLGPQYVCTNFWTFICTTCSGIHREFTHRVKSVSMAKFTSQEVSALQGGGNASAKEIYLKEWDPERNSLPDSSNVERLRTFIKHVYVERKFTGERSLNKPPRAKSGDADDFSENKKMDSYQGGSRSPPFNDTYERRYTDRPSPGGRSDDRNYRSSYDERRSPGSDGDYGRSPARTEIVNDWRRDDRFGNGKKSEDGRVGNGGLKVEATSPDFQRDLDSSSPPMVRPVRDILGENVSPLRVIEPPPKANGGKSADASLHTQRTASSSSLASSTGNPAEVKVESSLIDFDAVPEPPANSVAPTVTLNDNWANFDSFAEVKVSQAPSHANVLESALSDLMVPAPPGQTSATASANATSGNMSVLSTTNATMPVGYVSATDIGTIGPVAPASNSLAVPSGGIPSAAAGQTADFFLGVAGGQWNHQPFPHPTPGSQPPAQPFVPGGPLGVQVSRVDSGPLEAISGSLAQPSVVETRSVGKKELPEDLFTANYSTFPAPVPGWHTGPSQGLGSNMQYFGLNMQYNVPAPVPTYTQPSISSNPFDFNSEPTPAQASPFPSLLPLQGALPSVAAPTGLPHSSTFGTTTPAWMPDQYSNQTGMSSHAQTYGSSVAPSPYMGQPVTSNFPPRHQGAAGFGFGPAAFSSPAPNQQPGALYASPVPQNTFSSSGGNPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJG4</accession>
    <name>A0AAF0XJG4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DGS1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0727379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJG4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
            </authorList>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="230" mass="26345" checksum="BFA3DE47DDA0042D" modified="2024-05-29" version="1">MWNQERNNKGAKHNEPTFSLCCRDGMIELPAERHPPPYLDDLLSNGVKSEHFKKNIRTYNSMFHFTSMGGKIDRSVNDGGGPFCFKLNGQNYHLLGTLKPTDGENPKFCQLYIYDTENEVDNQIVAALVQMMDDHNKLASGFRMARQRFNLQEPEEFRLQLISSQSASGRANHVIASNEVAALIVGNVEDESPYRDLIVETKQLYLKRMMDFTLTFHYMVSRPGQQKMST</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            </authorList>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="69" mass="8113" checksum="DD4F90B2161F4F6B" modified="2024-05-29" version="1">MDVKQGISKTRDMVLYLVFEYMDTDLKKFIRSFRQTRENIHLPTVKTLMYKLCKGVAFCHGHGVLDKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPX6</accession>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class III subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="dcr:108199848"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013978">
        <property type="entry name" value="MEKHLA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF7">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08670">
        <property type="entry name" value="MEKHLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="148"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH12093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="92090" checksum="4D6DFE7B14E85EEA" modified="2024-05-29" version="1">MGESQMDCSKYVRYTAEQVEALERVYAECPKPSSSRRQQLIRDIPILSNIEPKQIKVWFQNRRCREKQRKEASSLQTVNGKLSAMNKLLMEENERLQKQVSHLVYDNGCMRTQLHTASPTTTDTGCGSMVAISQQQQQTSTSQHPHRDANSPTGLLAIAEETLAEFLRKAIGTAVNWVQIVGMKPGPDSIGSVAISRNCRGIAARACGLVGLEPRKVAEILKDRISWFHDCRCLDVSSVFPTGNGGTLELLYMQTYAPTTLASARDFWTLRYTTTLEDGSLVVCERSLTSSTGGPMRPPTACFVRADMLPSGYLIRPCDGGGSIINIVDHMDLNPLTVPEVLRPLYESSTILAQKMTMASLRHIRQIAQETSGEIKYTGGHRPAVLRAFRQRLCRGFNDAINGFVDDGWSTMNSDGLEDVTVAINMSAGKFLSSQYNAFSMLPTFGGVLCAKASMLIQDVPPPLLVRFLREHRSEWADSMIDAYNAASLKASPYAVPCARPGAPPSTQVILPLAHSMENEELLEVVRLENRAPEDLTLTSNMYLLQLCSGIDEKTAGGCAQLVFAPIDESFADDAPLLPSGFRVIPLDPESDKSSANRTLDLASALEVRHGGAHPHGEDNAGNYNLRSVLTIAFQFPFENHFRDNVASMARQYVRSVVGSVQRVAMAISPSRANFNLEPKQLPGSPEAFTLARWISRSYRVHTGAELLKTESQVGNAVLEHLWNHSDAILCCSVKTNISPVFSFANQAGLDMLETTLVALQDIMLHKILDDAGQKVLLSEFSKIMQQGFAYLPAGVCFSSMGRSISYDQAIVWKVLKDDNSDHCLAFMFLNWSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQT6</accession>
    <name>A0AAF0XQT6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylornithine deacetylase</fullName>
            <ecNumber evidence="6">3.5.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">N-acetylornithinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0831004</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(2)-acetyl-L-ornithine + H2O = L-ornithine + acetate</text>
            <dbReference type="Rhea" id="RHEA:15941"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="3.5.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine from N(2)-acetyl-L-ornithine (linear): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M20A family. ArgE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQT6"/>
    <dbReference type="KEGG" id="dcr:108192319"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008777">
        <property type="term" value="F:acetylornithine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08012">
        <property type="entry name" value="M20_ArgE-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000119">
        <property type="entry name" value="Acetylornithine deacetylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF3">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="16">
        <location>
            <begin position="205"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH11517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="48220" checksum="F09C32664221E6DB" modified="2024-05-29" version="1">MSSSSSLAQTLGDLNTQNFVTLLSKLIGESKYVQNNPPELVPEEDRIVNHVLESLLPLSTTTGGGPLIVNHVTYVSKRGNLIVEYPGTEPDKILSFVGMHMDVVPANPDEWNFDPFSLSTKGDELRGRGTTDCLGHVALVAELMRRLGETKPKLKSTVVAVFIASEENSSIPGVGVDALVKHGLLNKLKQGPLYWIDTADKQPCIGTGGAIPWKLHVTGKLFHSGLPHKAINPLELAMEALKVIQLRFYKDFPAHPKEEVYGFATPSTMKPTQWSYPGGGNNQIPGECTISGDVRLTPFYDISDVMKKLQQYVEELNTNIEKIDVRGPVSKYVLPDENLRGRIEISFEEAYPGVACNLDSRGFHVLCKATEQVVGHVKPYSITGSLPLIRDLQDEGFDVQTSGYGLMATYHAKNEYCLLSDMCQGYKVFANVIDQLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQZ0</accession>
    <name>A0AAF0XQZ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cyclin-dependent kinase</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0831842</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQZ0"/>
    <dbReference type="KEGG" id="dcr:108198362"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000545">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000104">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="4"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH12340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="334" mass="37769" checksum="6EDB985BF67986FD" modified="2024-05-29" version="1">MEKYKIIKEVGSGSFGSVFKAMNTQSGEVVAIKRLNKEFDSWEDCLNLREVKSLKKMHHANIVKLKEIIGENNFLFFVFEYMECNLYQVMSTRTKPFSETDVRNWCFQVFQGLAYMHERGYFHRDLKPENLLVSGDIIKIADLGLAREINCSPPYSEYVATRWYRAPEILLAAPIYGPAVDMWAMGAIMAELLTNCPLFAGVNQQDQMYRICSVLGTPTEVDWAYGIELADDISYQFPQHSGVSLSQLMPSASKDVVGLIQSLCSWDPCKRPTALEALQHPFLRSCYYVPPGFRFKTSGLGMNLSARSEGSVKHKYLSRIPGTFSNSGPIRCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSB4</accession>
    <name>A0AAF0XSB4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0831859</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSB4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH12357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="162" mass="18401" checksum="056094189B19B61A" modified="2024-05-29" version="1">MSSLNFLSHFYILFLVVASMAFFVTIYHCIMVSCQNRIERHNSDQVSQSTVIRIQNFLLWRNGQELNSAQDRGATVLGLENSKIELIPRHKFEKGKGILDAECAVCLSEFAEDEEIRTLPECLHSFHVECIDMWLHSHPNCPTCRANASPPSSSLLLHPCPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTH1</accession>
    <name>A0AAF0XTH1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0832039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12534.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUB1</accession>
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            <fullName evidence="3">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="4" type="ORF">DCAR_0934005</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUB1"/>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-haem dioxygenase N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="177" mass="20424" checksum="01C936B3AFBFBA7D" modified="2024-05-29" version="1">MATLHEAYEKWGFFQIENHGIDKQLMEKVKDLVNQHYEEKGNRTTIDRETVFFIRHHPDSNINEISNLSQEFRVTKCMNSKTRSLATDLQSLSMELREKQSTYLERLKQQKEGPDGVDLEMNLNRTQSGMEDADLDDMVGSPSGMDNLGFDRKLMPEDYLRFVSGSVGMFCLCLSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUY0</accession>
    <name>A0AAF0XUY0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0933308</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CAP family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH13797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000006">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018106">
        <property type="entry name" value="CAP_CS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01088">
        <property type="entry name" value="CAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="4">
        <location>
            <begin position="305"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH13797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="465" mass="49991" checksum="00BDBDE8D97B79D8" modified="2024-05-29" version="1">MEESLIKRLESAVTRLESLSDGLRSGASAATGGDAASDPSVVAFEDLMSEYVGRILKAAETIGGEVFDVTRVLEEGFSVQKDLIIQMKQSQKPDMAGLCAFLGPLNQVITKANEMTGGKRSDFFNHLKTVAESMSALAWIAYTGKDCGMSMPIAHVEESWQSAEFYNNKVLVEYRNKDPNHVEWAKAMKELFTPGLRDYVKAHYALGPVWSATGKTIQSAPKSSPAPPPAPSASLANSGTSKPSSSNSAGMSAVFNDISSGNVTAGLRKVTNDMKTKNRADRGGSVTTTEKESRPKSFSSAKTGPPKLELQMGRKWVVENQTGVKDLAIDDCDAKQTVYIFNCKDSVLQIKGKANNITIDKCNKMGVVFEDVVAACEIVNCNRLEVQCQGTAPTISIDNTTGCQVYLSKDSLGSSITTAKSSEVNMLVPGASPEDDMVEHPLPQQYMHVFQDGQFVTSPVSHSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XW97</accession>
    <name>A0AAF0XW97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0833116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XW97"/>
    <dbReference type="KEGG" id="dcr:108199378"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000017">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF14">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH13606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="860" mass="97319" checksum="B373C16FCB56DA51" modified="2024-05-29" version="1">MDSRSRVNFASLASGNLLDIPQIPSPRALPKVMTTPGVIPDVDGCGFNDAESDSGTSSSERKIIVTNMLPIHAQKDSETNKWVFTRDEDSLYLQLTDGFSPETEVIYVGSLKVEVDANEQDEVAQQLMDNFKCVPTFLPHDLQKKFYHGFCKQQLWPLFHYMLPMSPDHGERFDRTLWKAYVSANKIYADKIMEVVNPEDDYIWVHDYHLMVLPTFLRKHFNRIKLGFFLHSPFPSSEIYRTLPVRNEILRGLLNCDLIGFHTFDYARHFLSCCSRMLGLYYESKRGHIGLDYSGRTVYIKILPVGIHLGRLESVLSLSSTRTKFKEIQEKFKGKKVILGVDDMDIFKGISLKLLAFEQLLQQHQDLQGNLVLIQIVNPARSSGKDVQEVKRETYSTATRINEVYGSPDYEPVILIDRHVARYEKSAYYAMAECCIVNAVRDGMNLVPYKYIICRNGSADLDEAKGIEKDSPRTSMLVVSEFIGCSPSLSGAIRVNPWDIDSVAEAMYTAITMPDSERQMRHEKHYRYVSSHDVAYWARSFLQDLERACKDHYSKRCWGIGFGLGFRVVSLSPSFRKLSIDYIVPPYKRSSRRAIFLDYDGTVVPQSSLSKRPTPEVVYAINTLCNDPNNTVFIVSGRGKSSLSEWLAPCEGLGIAAEHGYFIRWNKISDWERSPLAIDLEWKEIVEPIMKLYTESTDGSYVETKESALVWHHQDADPDFGSCQAKELLDHLEKVLANEPAVVKRGQYIVEVKPQGISKGLVAEKVLSTMVSSGMPPDFVVCVGDDKSDEDMFESILSTVSSPSIPAPEVFACTVGRKPSKARFYLDDSVEVVKLLQGLANASNPKPRSSHVKVSFDNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1N1</accession>
    <name>A0AAF0Y1N1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0935585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y1N1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF357">
        <property type="entry name" value="IP12678P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="26"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH16036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="502" mass="55279" checksum="EE3F56E6F105DA81" modified="2024-05-29" version="1">MAGGGMVASSGGTEFEAKITPIVIISCIMAATGGLMFGYDVGVSGGVTSMDDFLKKFFPVVYRKTQADDDSNYCKYDNQGLQLFTSSLYLAGLTATFFASYTTRRLGRRFTMLIAGIFFIIGVVLNTAAQDLAMLIAGRILLGCGVGFANQAVPLFLSEIAPTRIRGGLNILFQLNVTIGILFANIVNYFTAKIDGWGWRLSLGLAGIPALLLTVGALLVVDTPNSLIERGKLAEGKEVLRKIRGTDKIEPEFLELVEASRIAKEVKHPFRNLLMRRNRPQLVIAIALQIFQQCTGINAIMFYAPVLFSTLGFKDNASLYSAVITGAVNVLSTVVSIYVVDKLGRRILLLEAGVTDHSDNLGTAYAVIVVVMICTFVSAFAWSWGPLGWLIPSETFPLETRSAGQSVTVCVNLLFTFVIAQAFLSMLCHFKYGIFLFFSGWVLIMSIFVVFLVPETKNVPIEEMTDRVWKQHWFWKRFMEDETDDGFKKNGHAKVFDPVAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AI50</accession>
    <name>A0AAF1AI50_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0102528</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AI50"/>
    <dbReference type="KEGG" id="dcr:108225821"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000043">
        <property type="entry name" value="sugar transporter ERD6-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF37">
        <property type="entry name" value="SUGAR TRANSPORTER ERD6-LIKE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="42"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG83353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="477" mass="50947" checksum="429458AFCFF55F26" modified="2024-05-29" version="1">MAIEHVKDIENGDYNKGVGDLEQPFLEWREGSSGSLGVVLFSTFVAVCGSFEFGSCVGYSAPTQSGIREDLNLTLAQYSMFGSLVTIGAMIAAFTSGRIADFIGRKGAMRMSAAFCIAGWLAIYLSVGALSLDIGRFLTGYGIGIISYVVPVYIAEIAPSNLRGGLTTLNQLMICTGSSIAFVIGTIITWRNLALTGVAPCFALLVGLFFIPESPRWLAKVGLEKESEVSLRRLRGKNAEITAEADEIQISVQSLRKLSKAKLTDLLEAKYIRSVIIAVGLMLFQQIGGINGISFYASQTFVAAGITGNVGTLAYALIQVPVTIIGAVLMDKSGRRPLLLVSATGTFLGCLLTGTAFLLEGQQLLLQWVPILAVSGVLTYIASYSIGLGPVPWVIMSEIFPLHIKGLAGSLAVLTNWLGAWAVSYTFNFLMSWSCPGTFFVFSGSCALTVLFVAKVVPETKGKTLEEIQASINSQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIE3</accession>
    <name>A0AAF1AIE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Molybdopterin synthase sulfur carrier subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Molybdenum cofactor synthesis protein 2 small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Molybdenum cofactor synthesis protein 2A</fullName>
            <shortName evidence="6">MOCS2A</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein MOCS2A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0103723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer; composed of 2 small (MOCS2A) and 2 large (MOCS2B) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MoaD family. MOCS2A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIE3"/>
    <dbReference type="KEGG" id="dcr:108201389"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
        <property type="term" value="C:molybdopterin adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990140">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000010">
        <property type="entry name" value="Molybdopterin synthase sulfur carrier subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03051">
        <property type="entry name" value="MOCS2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028887">
        <property type="entry name" value="MOCS2A_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01682">
        <property type="entry name" value="moaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="1-thioglycine; alternate" evidence="6">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycyl adenylate; alternate" evidence="6">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG84539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="104" mass="11498" checksum="CD7E6B0F7B31007D" modified="2024-05-29" version="1">MDVIQTYSATKDVDSLHEKENVLVQIKVLFFARARDLTGLTEMSLDVLQGSTTHDCLNKLIAKFPQLDEIRGCMVLALNEEYTTESATVKDKDELAVIPPISGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIU3</accession>
    <name>A0AAF1AIU3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0100826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIU3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="8"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="123" mass="13687" checksum="3111F657543C7178" modified="2024-05-29" version="1">MTSAKRQSALVVDDDPVTRIITRGLLNYFGFEVSTLNDGIEVVNMYEAGKGHFDLIITDMEMPVMNGIEATKKLRSLGVGCKIIGVSSCDDETSRRHFMEAGLDHLFYKPLNVTKLQSCLENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIW5</accession>
    <name>A0AAF1AIW5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0100901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIW5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001237">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF365">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="352"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="64509" checksum="A09D6F001344C80C" modified="2024-05-29" version="1">MARASSMTLKDLGRLRSHPLLSHSSSLLNPTLSFSSISSPCPSSNFYNTTPTPFNSINRILNLLETCQSINHFYQIQAHLLTSSLFQNPSFSGRILKLSADYNDIHYTLLLFKFISLPDTFCYNILIKACALSCVPHQAVILYFHMLKYQFLPNSYTFVSLFKACVQMGSVNVGLKCHGQAIKNGVQCVLQVENSLIHMYGCYGLIDVAKRLFGEMFVRDVVSWNTLVDAFVRVGDLSAAHELFDLMPHKNVISWNVLISGYLSGGNPGCGLKLFREMAGFKIKGNDTTMVSVVTACGRSARLKEGKSVHGYLIRTFVRLSLIINTALIDMYSKCQRTDIARKVFERLLTRNIVCWNAMILGHCLNGNPKDGLQLFAQMHTKTRLIESRIGDDNIIELGEQVLPDEVTYVGILCACLREGLLTEGINYFNQMTDVFGVKPNFAHYWCMANILASFGLMEEAMEILRNLSSFAELPKESSLWASLLGLCRFKGNVVLGEQIAKALIDKDPLNQSYYALLMTVYAVAGQWENVALTKETMKERGFKSMPGFTLADLKFIVHNLGVGNELHNRYIMLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJU7</accession>
    <name>A0AAF1AJU7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJU7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="70" mass="7834" checksum="3118F9CAA0C34B7C" modified="2024-05-29" version="1">MAAPDKSSSPDLIKKKVWSHEVAIGELNTLSSSRAVYQKNGNLYFRTSIVKAKAFEEKQLQKLQKQSSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AL44</accession>
    <name>A0AAF1AL44_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0205916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AL44"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF292">
        <property type="entry name" value="ESTERASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042225661" evidence="5">
        <location>
            <begin position="23"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG86698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="385" mass="42127" checksum="BC50E5D241FF5167" modified="2024-05-29" version="1">MGKYSAFSVFLLSILVAQQVSAADTCRFPAIFNFGDANSDTGAFAAWFFGNPPFFGQSFFGGSAGRVSDGRLLIDFMASSLGLPFLHPYMDSLGADFAHGANFAEILSTIALPPANNIIPGVRPPRGLNPINLDIQVSQFAQFINRSQTIRQRGGVFRQFMPKQEYFSQALYTIDIGQIDITQEFLKNKTDDEVKAAVPALIASLSSNIKILYSLGGRTFWIHNLGPNGCLPILLTLAPVPDSQLDSAGCVKRYNDLTQYFNSELKKGVDQLRKDLPSAAFTYVDVYTAKYSLYQEPAKYGFTHPLETCCGFGGRYNYGENSLCGSTITVNGTQRTVGPCANPAEYINYEGQTYTQAADQITFNKIASGELSDPPNSLRQACRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMF0</accession>
    <name>A0AAF1AMF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0105040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a hydroperoxy polyunsaturated fatty acid + 2 glutathione = a hydroxy polyunsaturated fatty acid + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:19057"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:131871"/>
            <dbReference type="ChEBI" id="CHEBI:134019"/>
            <dbReference type="EC" id="1.11.1.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMF0"/>
    <dbReference type="KEGG" id="dcr:108225074"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000025">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF118">
        <property type="entry name" value="PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE 6, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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</entry>
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                <person name="Senalik D."/>
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                <person name="Simon P."/>
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    </evidence>
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    </evidence>
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="518" mass="58816" checksum="E0E98763943FFA2E" modified="2024-05-29" version="1">MIRTSAKKLQFLLERLCTRVLNTFKFFKYLLRSFFDHLMFLFLHYVPPFWTHLLYFTFFSVSAFLFFKSSSYSSRPRDIDLFFTAVSAISLSSMSTVEMEVFSHFQLLILTFLMLIGGEVFLSLVKLQISKFEKKHTFPVTSLSEYSASNPELGLTEQDMTNTFEKSAEDRLRISSINCLSYSIVAYLLATHMISSFMISFYMESVPSAKQVLTQKGIKVSLFSVFAAISSFANCGFLPTNESMMVFKKNTTLLLILIPQCLVGSTLYPFFLQLLLSFLNTVTKRPELEYISKHYNKLGYTNLMSRRKSCSIAATVVGFVMIQFVIFAAMEWNNKGLFEGLDSYEKMVAILFQVTNTRHTGESVFDISTISPAVLALFVAMMYLPPYASLLSVKNGEKSSTIVDATASRRTKRKYVQLSELAYLAIFTILICISERKSIKEDPLNFSVFNIIFEVISAYGNVGFTMGYSCGRRIKSDESCKDAYYGFAGRWSDSGKGILILVMLFGRLKSLFYKGSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATA1</accession>
    <name>A0AAF1ATA1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0311500</name>
        <name evidence="8" type="ORF">DCAR_0311501</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATA1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47177">
        <property type="entry name" value="F18C1.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47177:SF4">
        <property type="entry name" value="OS06G0283200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="99"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG92237.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG92238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="580" mass="64008" checksum="23539A090C722133" modified="2024-05-29" version="1">MKQEPDEQPNSPNTQQKTVISQLLTTGKQKIKNETSCTNSSQSYVCDEGKSIKGYIHTCDNYQQMKDCALADELIVGVPAGDQAYHENIKTEHEDSYAEIQCNVCHNMSNQTLLLQCDLCDSSSHTYCVGLGDTVPEDDWTCQNCTEHAEDEQDLKAVGLSGIDSHSGSKNRCHQNVSSTEANLSIHDIVRESGPYNVERSLPNQSRSPLTNAGDDRTVLISCRNRDSRTRALRENWDKIRQGSLSFSSLPIIKPGELSCGTSSATKSSTSDIIPDQATQDIKKAWKMMKAAKSVEKKKYTNTIPCPSNGSKHPLTNTETPKHFPSVRSMLPSSRHSGDKDKDCKNLKRPFQGCATEKCHDKPQFPKFQKENPSSFRNFEHHPTTYSAESSNFSTKTGQFSVHARIDDESSVNLQTNRSLGSLANANKIHSSSNCSVSLRSVAKPLAAAKQNVHATAPFNVNKVTNVEEHQVDGQAKVLSDAKSEIQTLVKLNLKLSNAQKKLATNEFKEVARHATYSILAACGIGIPKPWVRPFSNPHCSHADKVDGARRSTLMPSSCRECFMAFVKDVVDTVLLEKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU88</accession>
    <name>A0AAF1AU88_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat stress transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0311976</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AU88"/>
    <dbReference type="KEGG" id="dcr:108214452"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="12">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
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    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
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        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000064">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF8">
        <property type="entry name" value="FLAVONOL 3-O-GLUCOSYLTRANSFERASE UGT89B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG97604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42284" checksum="FEA8269AB5AFDE9A" modified="2024-05-29" version="1">MIAALVGLYDPIVNWFRNHPSPPVAIISDMFFSWTNRLAGELKISRYVFSPSGILALSVVWTSWRDMPKRNDPKDENEVIKFSNIPNCPSYPWWKLSPLFRSYVQGKSQSELFRASSLENVASRGIIFNSFTELEKIYLDYLKKFLGHDRVWTVGPLLPSEEERVERGGSNEILAKEIKSWLDQFENQTVVYVCFGSQAVLTNKQMEMLALGLEKSGVRFLWSYKEPTKGHTVGEYGMIPSGFEDRVSGRGLIVKEWAPQVAILSHPALGAFLTHCGWNSVLESIAAGVPMLTWPMGVDQFTNANLLEELRVGTKVCEGEKTVPDSDELARLLASSVGDAKGVRIARAKVLSKAARDSTGNDGSSYRALDSIVDQLSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AXJ7</accession>
    <name>A0AAF1AXJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">allene-oxide cyclase</fullName>
            <ecNumber evidence="3">5.3.99.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0415390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate = (9S,13S,15Z)-12-oxophyto-10,15-dienoate</text>
            <dbReference type="Rhea" id="RHEA:22592"/>
            <dbReference type="ChEBI" id="CHEBI:36438"/>
            <dbReference type="ChEBI" id="CHEBI:57411"/>
            <dbReference type="EC" id="5.3.99.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the allene oxide cyclase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.6" evidence="3"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AXJ7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046423">
        <property type="term" value="F:allene-oxide cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009410">
        <property type="entry name" value="Allene_ox_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034871">
        <property type="entry name" value="Allene_oxi_cyc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31843">
        <property type="entry name" value="ALLENE OXIDE CYCLASE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31843:SF11">
        <property type="entry name" value="ALLENE OXIDE CYCLASE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06351">
        <property type="entry name" value="Allene_ox_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141493">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG96060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="207" mass="23171" checksum="AD45EC89B4230EC8" modified="2024-05-29" version="1">MNKIFQKLEENFQFPIIEKVVTVRTNICAKYLKFKVQELSVYEMNERDRSSPAFLKLSQKSTNSLGDLVPFTNKIYTGDLQKRIGITAGLCILIQNVPEKKGDRYEAVYSFYFGDYGHITVQGQYLTYADTYLAVTGGTGIFEGVSGQVKLQQLIFPFKLFYTFYLKGIPDLPQELLGQAVAPSPTVEPTAAAKACEPGSTIANYTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYJ7</accession>
    <name>A0AAF1AYJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="19">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0416464</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="19">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and several low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="19">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="19"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYJ7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
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            <end position="20"/>
        </location>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
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    <sequence length="238" mass="26013" checksum="40C342264F1E1110" modified="2024-05-29" version="1">MSIFAGIFLASLCSVPFSKQLFVHCLISDAITGKNENLSIPSIPPTVSAVKNPNSKIEYDISNHERFPPGDPSKRATAYLVLTGGRFVYASLIRLLVLKFILSMCASKDVLALASLEVDLSSIQPGTTVTVKWRGKPVFIRHRTQEDIQAAAKVDVNSLRDPQEDGVRVKNPEWLVVIGVCTHLGCIPLPNAGDFGGWFCPCHGSHYDISGRIRKGPAPYNLEVPTYSFLSENTMLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B600</accession>
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        </lineage>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="407"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="506"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="42"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="582"/>
            <end position="661"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
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            <end position="148"/>
        </location>
    </feature>
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        </location>
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            <end position="235"/>
        </location>
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            <begin position="251"/>
            <end position="263"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
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        </location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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        </location>
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        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH07475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="661" mass="70656" checksum="7AAB134915870FAD" modified="2024-05-29" version="1">MQSKEDESSTSSDDDYSSEDEPNVCFYLFLYLVKFRIYVAPQKTASKTKQSKKEDSSTSSESDSSSEEEPNNKPAAQQPVPKVAQQKTESKSVQSKKEESSTSSDDDSSSEDEPNVNPAANQAVQKPNDSSSDEDSSSSNGTVTSVVVAKNGTSGKHATSKVADSSSDDSGSDSEGGKNLVKTQSPDINQLRISRSVHVFDSDGGDSGGDAVAAKMDVSSEESDSDDSSDSDDDELKAKPRRKQVPVGALLKKDSATSNKKDESSDDSESSDSSSSDDDSPVPNAALSEKASSRTASKRKLSTKVDNEEDSSSESEEEEPKKKKMKVQLYPTVLKSVKKESSSEESSDDGTSKDEEKKVPKTPRQKDADEVVVDAESVKTETKKTSISYALQPQTPGALQVKASGTKTLFVGNLAFSVERADVEDFFKDVGEVADVRFAVDGDGNFRGFGHVEFSTIEVAQEAMELNGQTLLGRPIKLDFARERGAYTPSSGKENSYQKGGPTQVHTIFVRGFDTSGDENKIRIALKQHFETCGDISRISVPKDYESGAVKGIAYMDFKDSDGFKKALGLNDTEFGESYLTVAEAKPRGDSGGETRGGRSGGRFGNRSGGDKFGSHDGGDRSRGVRGGRSVRGGTRGRGRGGPARPSMATVGTGKKTVFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B605</accession>
    <name>A0AAF1B605_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0623885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B605"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="LEA_2"/>
    </dbReference>
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        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415:SF9">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH04476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="220" mass="24213" checksum="ECD4AFAD8D3DC155" modified="2024-05-29" version="1">MAEDPSVGPSKGKLKGSKGNAGKTWRSVSQFILIFLVLIAVTALVLWLVYNPHKPKFSLVNAAVFDLNTTSPPFISTTLQFTIVARNPNDRVSVYYDRLNAFVSYKSQMITPQMILPPLHQYKDSTVSFSPVLGGVAVPVSLEVANGIVMDMEGYGLVQLRLVLQGRVKWKVGPIKTSYYDLYVACDMLLGLKKGIVGQVPLLGISHDRDQDHHGCHVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7N1</accession>
    <name>A0AAF1B7N1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0727106</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7N1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138">
        <property type="entry name" value="OS01G0690200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138:SF27">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042237927" description="non-specific serine/threonine protein kinase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="8">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase C-terminal" evidence="9">
        <location>
            <begin position="158"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH07673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="28830" checksum="8FF6405F4BF5A5A7" modified="2024-05-29" version="1">MRRGLLCCMFFGICIISLPFTTFCKEDEQFEKCKKAGYCGNQTIRFPFYTEDIPEFCGFRGFNLTCLNNQVLLLNISEDQYRITQIFYSNNSFRVSNVLSSRSGFCSLGKIRNLSLPSDHRFELDSTSNLTLLSNCTSDFARIFSRSKVGCDVGKNDTDSVLAMKTNDPGLNYANKECKTVAVAPVLDYTEESTNYLKLIRDGFDLKWVATDCRDCQDSGGYCGFEGGSVNKFKCFCKDRPHSRSCKNSKQPHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7Q1</accession>
    <name>A0AAF1B7Q1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0626351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7Q1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="81" mass="9630" checksum="01BDFA2C2C455F6C" modified="2024-05-29" version="1">MWSKGVRLVRGRERRMTRHSSKSRNNMRMLRKLQRMITPDQSQVADPDFLFEQTAAYISLLQSHVSSLKKIIHHVQQKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B849</accession>
    <name>A0AAF1B849_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0626214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B849"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF124">
        <property type="entry name" value="ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH06786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="15762" checksum="0FA385312B274731" modified="2024-05-29" version="1">MSSLGSAPALCAKACGFYGSSAKRNLCLCSKCYQVVLKQEFALCESYTKSIAASLRELSLRKENHQDGVSDDGFVAKKKACRCSCCDKKIGLLGFACRCGGKFCNMHRYPETHMCFFDYQALGRASLARENPFVGRDKLGERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8L2</accession>
    <name>A0AAF1B8L2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0727347</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B8L2"/>
    <dbReference type="KEGG" id="dcr:108195575"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1551">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="481" mass="53718" checksum="FA1A822B775D1F9A" modified="2024-05-29" version="1">MSSPERSAKKQAHVVCIPSPSQGHIKPLLKLAKLLHYNGICITFVNTEFNHKRFLKSGGLYSLDGLPGFRFESIPDGIPLSDPDSTQDPASLVHAIVNNLLPPFQDLLTKLSTQTPPVTSILSDGFMPFTADAAQSIGIPIVLVWTFAACAFMAFYHFKSFLEKGLVPLKDESCLTDGYLDTTIDWIPGMRNIRLGDLPSHVRITDPNDVIFKYIMDSTEGARNAKGVVLYTFDDLEQEVVSAISSALPHIYTIGPQQLLLNQIAPDHEEKLKGIRDSLWKEDEKCLQWLESKEANSVVYVNFGSTTIMAPEKLVEFGWGLANSNHRFLWIIRPDLIVGDSETTLGLEFMEAIKDRGFISRWCPQEKVLNHVSVGGFLTHGGWNSVTESLSAGVPMLCWPFFADQLINCKYICTEWECGMEISNDVKRDHVEKLVRTLMDGVEGKRMKKKAMEWKIMAEKACSPAGSSSQNLDKLVHLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAZ4</accession>
    <name>A0AAF1BAZ4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108197783"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH12934.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="60" mass="6992" checksum="E4084684B1B79495" modified="2024-05-29" version="1">MPHRTRPMAALLLFTGLNVVLVSTITPVYDFVCFHPYWERRRERRRQEREAASASSAIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="6">Phosphoglycerate mutase-like protein 4</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0729547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH10086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="His_PPase_superfam"/>
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    </dbReference>
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            <position position="23"/>
        </location>
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        <location>
            <position position="98"/>
        </location>
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        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH10086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25547" checksum="5F0CF986A79D1257" modified="2024-05-29" version="1">MYLRFSWKMTGGCNYAEIIVIRHGETEWNADGRIQGHLDVNLNEVGRQQADTVAARLSREPKISAVYSSDLSRAHETAEIIARNCGGLEVVKDRDLRERNLGALQGLVYHEIAKINPEAHKAFVSHSKDQEIPGGGESLNQLYRRATSCLQKIGEKHKGERVVVVTHGGFIRALHRRATTHHRVGKVLNTSVNVFHLSDGGKWSIKTWGDVSHLSETGFLKSGFGGDSNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BBX4</accession>
    <name>A0AAF1BBX4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0730080</name>
    </gene>
    <organism evidence="8 9">
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PDCD4 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="dcr:108195041"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ribosome binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Programmed cell death protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000009">
        <property type="entry name" value="programmed cell death protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039778">
        <property type="entry name" value="PDCD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626">
        <property type="entry name" value="PROGRAMMED CELL DEATH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12626:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="MA3"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="126"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="290"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="425"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="588"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH10611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="716" mass="79393" checksum="BEFF05CAC853F667" modified="2024-05-29" version="1">MASSEGFLTDEQREMLKIATENADVFSTSPRSPSGLKSPTRLMAARPIKAPANYKTAAEICVRHVRRTRSGKYVRVKKDGGGGKGTWGKVLDTGAEYRIDRNDPNYDSSEEPYLLIGSIVNDPLDEYKKAVVSIIEEYFSTGDVDVAASDLRVLGSGEYHPYFLKRLVSMAMDRHDKEKEMASVLLSALYGDIIPSTQISQGFFMILESADDLSVDILDAVDILALFIARAVVDDILPPAFITRAKLALPESSKGFQALQTCEKSYLSATHHAELVVRRWGGSTQVTVDEVKKKIANLLKEYVESGDTSEACRCIRELGVAFFHHEVVKRALIQAMEIPAAETAILKLLKEAAVEGLVSSSQMVKGFARLAERLDDLALDIPSAKTWFESLVPRAITQGWLDASFLKSFEEIGEMNQEVVEKMKQYKEEVVTIIHEYFLSDDIPELIQSLKDLGEPEFNPIFLKKLITIAMDRKNKEREMASVLLSSLHIELFSTEDIVSGFVMLLEAAEDTALDVVDASTELSLFLARAVKDEVLAPMNLEEIGWRLPPNCSGSETLDMAKSLVAARHSGERILRCWGGGTGWIIEDAKDKIVKLLEEYEIGGVLSEACRCIRELDMPFFSHEVVKRALVMAIENNNDKMLDLLDECYCEALITTNQLTKGFTRVSDTIADLALDIPGAEERFNNYVGYAQKKGWLLPSYGSSDTDSPLLSAAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDK5</accession>
    <name>A0AAF1BDK5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0935026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDK5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH15485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="159" mass="18303" checksum="740B1ECB49FD0105" modified="2024-05-29" version="1">MDSRDTQMQKPPRKRKLRILDVDSDRDESTPPSVNISNTETIDPANSGKKAETNVYKRKRLVKCSNYVPTLQFNELVEEKVNPLPELDEMMIQDMNKTTEIANTEERIMTQEVSTQLQQAAGNLDMVIYQPLISVNPIHEVPVEKNLQSKNLNIKHDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WX84</accession>
    <name>A0A161WX84_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">chlorophyll(ide) b reductase</fullName>
            <ecNumber evidence="13">1.1.1.294</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_012071</name>
        <name evidence="17" type="ORF">DCAR_0313680</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>7(1)-hydroxychlorophyllide a + NAD(+) = chlorophyllide b + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24768"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83356"/>
            <dbReference type="ChEBI" id="CHEBI:83357"/>
            <dbReference type="EC" id="1.1.1.294"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>7(1)-hydroxychlorophyllide a + NADP(+) = chlorophyllide b + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83356"/>
            <dbReference type="ChEBI" id="CHEBI:83357"/>
            <dbReference type="EC" id="1.1.1.294"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.294" evidence="13"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WX84"/>
    <dbReference type="STRING" id="79200.A0A161WX84"/>
    <dbReference type="Gramene" id="KZN03315">
        <property type="protein sequence ID" value="KZN03315"/>
        <property type="gene ID" value="DCAR_012071"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214174"/>
    <dbReference type="OMA" id="VQPCRSF"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034256">
        <property type="term" value="F:chlorophyll(ide) b reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010304">
        <property type="term" value="P:PSII associated light-harvesting complex II catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000223">
        <property type="entry name" value="Chlorophyll(Ide) b reductase NOL, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052625">
        <property type="entry name" value="Chl_b_Red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314:SF21">
        <property type="entry name" value="CHLOROPHYLL(IDE) B REDUCTASE NYC1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0881">Chlorophyll catabolism</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZN03315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG94385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="502" mass="55170" checksum="5DDC4FE126F2D554" modified="2016-07-06" version="1">MATVAKLHISPLDCHHRTGQPPVFHRQVAMWDPISLKGRRSFVQKCKSFRQEKESEVVDARKEMESSEVKSESGVYRLMGSLKSAIQRASRSRVDKKFVEDLEESLSSIALHVGRYIVTMMGTGVILLTGFQLSGGDGQMNDLIWYSWLGGIIIGTMIGSNMVLDEVARAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSQDSVETTIKELEDSLKECLETADDFSKRSLKYAEVVGVACDVSDPKDVKKLSDFAINELGSIDIWINNAGTNKGFRPLLQFSDEDIQQIVSTNLVGSIICTREAMRVMADQHKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKESKKSKVGVHTASPGMVLTDLLLSGSTVQNKQMFNIICELPETVARTLVPRMRVIKGNGKAINYLTPPRILVALVTAWVRRGRWFDDKGRALYASEADRLRNWAESRTRLSITDAMESYTEDTWVSVFSLSVVCAFIILSSTLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y7S3</accession>
    <name>A0A161Y7S3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0729408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
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                <person name="Macko-Podgorni A."/>
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        <source>
            <dbReference type="EMBL" id="WOH07136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23430" checksum="D68FB3A07F965ED5" modified="2016-07-06" version="1">MSDLTTTDSATIMDPKPLHPLHQIAENPTHKLLLKQWLKEEELILTRVSNKETQIDSIRKEITQLYCLFFLFHSTSLILLFTTSSSFSGSTKGCQRSWIPSVCSLVCSLGIIWAVRYKTDVEAHLERMLEREKEDGKLLGKCVEELRKKGAEFDLLKEVDALRKAKSLRVESRTVKKWEARDFVTLFFVGVACLVLGLTRVILCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AEU4</accession>
    <name>A0A162AEU4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein TRIGALACTOSYLDIACYLGLYCEROL 5, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008847</name>
        <name evidence="3" type="ORF">DCAR_0309998</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AEU4"/>
    <dbReference type="STRING" id="79200.A0A162AEU4"/>
    <dbReference type="Gramene" id="KZN00093">
        <property type="protein sequence ID" value="KZN00093"/>
        <property type="gene ID" value="DCAR_008847"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211317"/>
    <dbReference type="OMA" id="PMNILGL"/>
    <dbReference type="OrthoDB" id="1305999at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990052">
        <property type="term" value="P:ER to chloroplast lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010441">
        <property type="term" value="P:guard cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009667">
        <property type="term" value="P:plastid inner membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009666">
        <property type="term" value="P:plastid outer membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053288">
        <property type="entry name" value="TGD_Bridge_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34201:SF12">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN00093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="9746" checksum="7C9DBE44E9A17391" modified="2016-07-06" version="1">MVLTNFQGAGIGFGFGVGCGFGVGWGFGGMPMNILGLGAGGGCGVGLGLGWGFGTGFGSQYRSSKLTFQGMEFNKNSVDQDKDLDITKSTFKAGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AFV6</accession>
    <name>A0A162AFV6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_009045</name>
        <name evidence="7" type="ORF">DCAR_0310235</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AFV6"/>
    <dbReference type="STRING" id="79200.A0A162AFV6"/>
    <dbReference type="Gramene" id="KZN00291">
        <property type="protein sequence ID" value="KZN00291"/>
        <property type="gene ID" value="DCAR_009045"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210633"/>
    <dbReference type="OMA" id="KHIRSDD"/>
    <dbReference type="OrthoDB" id="828272at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044604">
        <property type="entry name" value="FLZ12/13/14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208">
        <property type="entry name" value="OS02G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47208:SF1">
        <property type="entry name" value="OS02G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="5">
        <location>
            <begin position="175"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="175"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZN00291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="248" mass="26919" checksum="1ADFE27E3288BBBE" modified="2016-07-06" version="1">MAELKGTKRRLSINLSFLCLNDQTQTPLNLPTKSSKKFENGQDGVVGLGIVAAMTQLDQPQDPLISTRPGFLGVSPRSNPIPIFGNMGDSKPAGSNFDDDMELSEEYTCVISHIGNNLVKKREYFDGKLTNKCESIVAKKGGSYWVSSGIFTEASPPSMSNAGESAAMAVFGTADFLSSCYLCKKKLHGLDIFMYRGEKAFCSAECRYKQISSDELKEKRRSGVKKQQEYSVSPCSGSIQFSSGVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AYD9</accession>
    <name>A0A162AYD9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0209537</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AYD9"/>
    <dbReference type="Gramene" id="KZN07603">
        <property type="protein sequence ID" value="KZN07603"/>
        <property type="gene ID" value="DCAR_008440"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208551"/>
    <dbReference type="OMA" id="WAKDKEF"/>
    <dbReference type="OrthoDB" id="687943at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009610">
        <property type="term" value="P:response to symbiotic fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000067">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF408">
        <property type="entry name" value="PHYTOCYANIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG90294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="16724" checksum="7DD5C7DF11FCFC54" modified="2016-07-06" version="1">MASIKIFVALALVSAIIAPALATDFVVGDDAGWKTNFDYKTWAADKEFHVGDKLIFNYPAGVHNVHKADLASFQSCTPAATSVALTTGSDVITLASEGKKWYLCSIASHCASGNMKLAITVLPQGESPAPAPEVSAATGFGGSTHMLWIGAAFATLMMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AZS3</accession>
    <name>A0A162AZS3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_001228</name>
        <name evidence="5" type="ORF">DCAR_0100644</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AZS3"/>
    <dbReference type="Gramene" id="KZN08163">
        <property type="protein sequence ID" value="KZN08163"/>
        <property type="gene ID" value="DCAR_001228"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN08163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG81495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="267" mass="30917" checksum="CF1C25880F253C8D" modified="2016-07-06" version="1">MYSGFERKINISVGVEDFPDWIIDEDGDASTSVNSFEETSDGDASLSVNLRPNLSHNYLGMILCFDLFSVYSVKTSASNIFESYTFSSNIVIVPRSIFRVTDTDHTITFASTVDRRWIHLLYKNEDNSITLNVADEGNTSSLRPVKQGTRAVTVDFVARTCDRRTEKYLETYNHPLGALKGRDYWQVHSIDEMLPPDIPHKLRGRPKKLRRREEWEGGSRCRSSQQQGTNLQRFNSKRRMHCRRCGEDGHQKNKCRKKHHYETTRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B3Y0</accession>
    <name>A0A162B3Y0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_002339</name>
        <name evidence="10" type="ORF">DCAR_0102428</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B3Y0"/>
    <dbReference type="STRING" id="79200.A0A162B3Y0"/>
    <dbReference type="Gramene" id="KZN09683">
        <property type="protein sequence ID" value="KZN09683"/>
        <property type="gene ID" value="DCAR_002339"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205107"/>
    <dbReference type="OMA" id="VMMFTFI"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF97">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="8">
        <location>
            <begin position="347"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN09683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="564" mass="60568" checksum="435CE1B2A60F96FB" modified="2016-07-06" version="1">MAAVVKGGTRPPWVGLGAAVWVQIAAGNAYTFPLYSHSLKSVLGFTQQQLTILGVANDIGENVGILPGIASNKFPPWVVMFVGVFAAFFGYGVIWLAVSQTVPSMPYWVLWVALVIATNSSAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTEVHSMLLGGSAENLLLFMTIGIPVTCLAMMYFVRACTPALGEDSSEHVHFLFTQVSSLALAVYLLTTTILKSIVPISRSISYTLIGIMVVLLMSPLAIPIKMTLFPASSKKIGKQASSSDDIVTEDGESDLTGPLLSSFSSDINLLGLYENEDVSEVDILLAMGEGAIKKKRKPRRGEDFKFREAVIKADFWLLWVVYFLGVGSGVTVLNNLAEIGVSLGVNDTTILLSLFSFCNFLGRLGAGVVSEHFVRSKAIPRTLWMTVTQVLMIIIYLLYASALHGTLYAATALLGICFGVQFGIMIPTVSELFGLKDFGIIFNFMQLGNPIGALLFSGLVAGYVYDSEAAKQLSSSCLGPTCFRLTFLFLAGVCGLGTILSIVLTIRIRPVYQMLYAGGSFRLPNGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164T923</accession>
    <name>A0A164T923_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0728266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164T923"/>
    <dbReference type="Gramene" id="KZM87221">
        <property type="protein sequence ID" value="KZM87221"/>
        <property type="gene ID" value="DCAR_024355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="2">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="3">
        <location>
            <begin position="285"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH08817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="496" mass="56877" checksum="0FBE1683EBF246C3" modified="2016-07-06" version="1">MAFTTSTPVNKLDPSKIKWEIKVRAQAVWKGITRDTKEFRGLNILFIDDSNSRIHAFIGARISSIFEPSLEEGQMYHICSFSVHRYTGYESHRCVRTDLHIYFSDYTSMQKITVSVPRIPNYNFDFYDLSSLGSTNDEKRFLFDVIGVLGDREPLLKYMSESKEEKMQLKCSITDGRITFFDEFALKFDKALEKQADEITIVIIGCGKIGHYLGELNISNYPATRFFINVDHHSVRMMKLWSLKPTFRQKVVVKKDEPRDVLLSVACIKDLKEEFNERRVCCSVKVEKVEEVQWFYHVCPKCSEELVAIDGRFKCTKYKRYIPYPERRFRISVICSDESGVIFIALGDSEVRKLIKKNVFEVDLDHAKESKDSDFPAVFKQFAGKVHSITLKINLKNVIKKSEVYESVDIAELSGISSATISNIEPSGETDDNRGDTVTEMEEGNEEESPVKSLESVSKDKIRKNVKVNTSTVTDGDDDFVLIELISEGKSKKCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TWK6</accession>
    <name>A0A164TWK6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HXXXD-type acyl-transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_025151</name>
        <name evidence="3" type="ORF">DCAR_0728877</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TWK6"/>
    <dbReference type="STRING" id="79200.A0A164TWK6"/>
    <dbReference type="Gramene" id="KZM88076">
        <property type="protein sequence ID" value="KZM88076"/>
        <property type="gene ID" value="DCAR_025151"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194866"/>
    <dbReference type="OMA" id="GKITMYP"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF12">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM88076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH09420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="49707" checksum="52968CDE74A13ADA" modified="2016-07-06" version="1">MSSPTIQQISECFIKPSHLSQDSKQVIPLATWDLAMLSYHYIQKGLLFKKPDAQDNFTQVFLQKLKDALSLTLAHFYPLAGRLAKKQDSHSFALFVDCVNSPGARFVHSSVDTTVSDILSPPYVPIIVQSFFDHHKAVNYDGLNMSLLTVQVTELIDGIFIGCSVNHSVSDGTSYWNFFNTLSRNFQGSGVTSQPIHDRWFPDGHGPFLSLPFTRDDQFISRYEAPILQERIFHFSVANLARIKAKTNALCKDRAVRISSLQALSAIIWRCLTRVRGLTKDQITSCCMSANNRLRLDPLLSTNYFGNCVQVLKTFTTASKLLENNLEWAALQLNQTIVQHDDKSVRESVATWLKTRHPKQLGLPDDPGNIVISSSPRFNMYGNEFGFGEPVAILCGYANKFDGKVTLYQGSEGGGSIDAAICLNPKIMSALECDEEFLDALNSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UMJ0</accession>
    <name>A0A164UMJ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_026163</name>
        <name evidence="10" type="ORF">DCAR_0730039</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the oleosin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UMJ0"/>
    <dbReference type="STRING" id="79200.A0A164UMJ0"/>
    <dbReference type="Gramene" id="KZM89088">
        <property type="protein sequence ID" value="KZM89088"/>
        <property type="gene ID" value="DCAR_026163"/>
    </dbReference>
    <dbReference type="OMA" id="HAPHRTI"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012511">
        <property type="term" value="C:monolayer-surrounded lipid storage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050826">
        <property type="term" value="P:response to freezing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010344">
        <property type="term" value="P:seed oilbody biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000136">
        <property type="entry name" value="Oleosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203">
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    </evidence>
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</entry>
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        </source>
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        </source>
    </evidence>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000564"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="130" mass="14852" checksum="EE280EBDA5326BD8" modified="2016-07-06" version="1">MSLSLSNPFLQAHLPPPSLSLKKDVCQTRPYTPRWTCRKPDIHPKFYDDAKVYCNGQLVMTTGGTKQEYVVDVWSGNHPFYQGSRSALLLDDDQVEKFRKKFSGLDSIMTIPDLKGEIILPPKRKAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X0Q8</accession>
    <name>A0A164X0Q8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prolamin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_020097</name>
        <name evidence="5" type="ORF">DCAR_0626357</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X0Q8"/>
    <dbReference type="Gramene" id="KZM92538">
        <property type="protein sequence ID" value="KZM92538"/>
        <property type="gene ID" value="DCAR_020097"/>
    </dbReference>
    <dbReference type="OMA" id="YIPLHAT"/>
    <dbReference type="OrthoDB" id="1368054at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040220">
        <property type="entry name" value="DD11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31207">
        <property type="entry name" value="ECA1 GAMETOGENESIS FAMILY PROTEIN (DUF784)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31207:SF35">
        <property type="entry name" value="PROLAMIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854207" description="Prolamin-like domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Prolamin-like" evidence="3">
        <location>
            <begin position="58"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM92538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH06928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="15333" checksum="D9191220DDF9552A" modified="2016-07-06" version="1">MAAKSATLSPMVFFFTLCIFTLAPSLVSSLQNEYIPLHATSPSSDNNLSSEFVRFVANCGENLTNTCGREIKNALLGLVEVSRPCCGELVQMGWKCHKGMVRLATSAMDNRDEASTIISNSARVYFRCGKIVESSAPSPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZJ06</accession>
    <name>A0A164ZJ06_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0522021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZJ06"/>
    <dbReference type="Gramene" id="KZM95997">
        <property type="protein sequence ID" value="KZM95997"/>
        <property type="gene ID" value="DCAR_019239"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223391"/>
    <dbReference type="OMA" id="CGFTKSC"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024593">
        <property type="entry name" value="DUF3444"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056988">
        <property type="entry name" value="Zn_ribbon_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44137">
        <property type="entry name" value="BNAC03G44070D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44137:SF7">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11926">
        <property type="entry name" value="DUF3444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23551">
        <property type="entry name" value="Zn_ribbon_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH02632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="800" mass="88222" checksum="2BFDE09D01BF244E" modified="2016-07-06" version="1">MKPKQQEAHKARDNALKLFAVKDFVGAKNYALEAYMLCPELEGISQMVTTFDIYCAAERKFDGEVDFYSVLGLEPSVEKSKLKKQYKKLAVLLHPDKNKTVGAEGAFKLVSQAWALLSDNAKRRSYDMSRSKRMSCGVLQSSMSSVQGSQVTGFDNGSKSLSSQSKLDTFWTLCTSCHVQYEYLRKYVNKKLSCKNCRGIFIAAETGPPPVSASFPYSPWQYPPDNGYGSHGYNGAHITTSAAGLTGKTVPGIHSGHGSESNSNLSFQWGSCPGTSAGIVEHSGLIAKSANSVHQPNGVSITNANGKHIISNGHMGATAPRRGRPPKKLKVDMCHTTPRESEVIASNVKSEVKYNNAKISAQGDNSNKIFSIAPVFDARKLLIDKARTVIRNKLEELRSSSAAAGEAPSEATTVAGLNRRAEARASVSIPITVPDSDFHDFDKDRAEECFQPKQIWALYDEEDGMPRLYCLIRQVISVKPFKVHISYLGSKTDTEFGLVNWLDSGFTKSCGHFRAFNTDIIDQVNIFSHHLSREKAGRGGCVRIYPKCGDIWAVYRNWSPDWSRTTPDSVRHQYEMVEVIDDYSEELGVCIAPLVKLDGYKTVYQRNTDKNALRWIPRREMVRFSHQVPSCALKGIATNLPDGCWDLDPAATPDELLQVEAGIHAEKAAHTKSCVGTPLHVFEPETGVLGGESPHFQEDNLDATPELVEKLINLQVETTPDKVIQDLNKGKVQKTPVEVLQELSEVQGEKPDHVESSLTSMDPLNPVKEALPSFQVEHPAALPSFQVENPGATSQSFFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZVS4</accession>
    <name>A0A164ZVS4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0522562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZVS4"/>
    <dbReference type="Gramene" id="KZM96485">
        <property type="protein sequence ID" value="KZM96485"/>
        <property type="gene ID" value="DCAR_019727"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221878"/>
    <dbReference type="OMA" id="QHAYAHE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043904419" evidence="2">
        <location>
            <begin position="26"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="441" mass="40714" checksum="BF7204D5452BB8BC" modified="2016-07-06" version="1">MKFRPLNFLAFLLVAFLFTDTFVSAEIYQIEELSEADMRHVLGRYGGYGNFEDKWGKGYGHHHGGGGHKGGHGGQPGGGGYGGQPSGGGAQPGGDVGQPGGPQPGGNVGQPGGYGGQPSTGGAQPGGSQPGGDVGQPGGSQPGGSQPGGDVGQPGGTQPGRSQPGGDVGQPSGTQPGRSQPGGDVGQPGGTQPGGYGGQPGTGGAQPGGNIGQPGGSQPSGSTGKPGCDQSGGYGGQPGSCGSQSGGDVGGSQPGGGSAQPGVDVGQPGGSQPGSGVGKPGDDQSGGYGGQPSTGGAQPGGDVGGSQHGGGCAQPSESCAQPGGDVGQPGKSGPQTGGDVGQPGIGGQPGADAGGQPGGSQPGGSQPGGDVGQPSGYGAQPSTTVPQPSQSSGYGGQPTGPQPIQQGGYGGQSSTGGDVGLASGGYGSPPGLGGAKPGGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZWS0</accession>
    <name>A0A164ZWS0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_019778</name>
        <name evidence="16" type="ORF">DCAR_0522622</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an important role in the biosynthesis of the phospholipid phosphatidylcholine. Catalyzes the formation of CDP-choline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>phosphocholine + CTP + H(+) = CDP-choline + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.7.7.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:18998"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="9"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZWS0"/>
    <dbReference type="STRING" id="79200.A0A164ZWS0"/>
    <dbReference type="Gramene" id="KZM96536">
        <property type="protein sequence ID" value="KZM96536"/>
        <property type="gene ID" value="DCAR_019778"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220243"/>
    <dbReference type="OMA" id="NDEITHN"/>
    <dbReference type="OrthoDB" id="17102at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000102">
        <property type="entry name" value="Choline-phosphate cytidylyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF56">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="14">
        <location>
            <begin position="34"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZM96536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH03226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="302" mass="35376" checksum="A2262D07D1E0C9C0" modified="2016-07-06" version="1">MPEKIQGPEEPAKVYRSCHPPPNPPPINRPVRVYADGIYDLFHFGHAGSLEQAKKSFPNTYLLVGCCNDEITHKYKGKTVMTELERYESLRHCKWVDEVVPNAPWVLTKEFLDQHEIDYVAHDSLPYADTSGAANDVYDFVKSIGKFKETKRTDGISTSDIIMRIVKDYNQYVIRNLDRGYSRKELGVSFVKEKRLRVNMKLQMIQEKVKEHQEKMRTVVKTAEMHRNEWVDNADRWVAGFLEMFEERCHKMGTAIRDRIQKRLKREQARVRVATNNGRSIDDDQEVYYDDNGVEEYYESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XA16</accession>
    <name>A0A165XA16_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0416229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XA16"/>
    <dbReference type="Gramene" id="KZM97965">
        <property type="protein sequence ID" value="KZM97965"/>
        <property type="gene ID" value="DCAR_014673"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217314"/>
    <dbReference type="OMA" id="MADDCAV"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000096">
        <property type="entry name" value="Basic helix-loop-helix (BHLH) DNA-binding superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF26">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG96891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="26935" checksum="44A6C1DA297627C8" modified="2016-07-06" version="1">MESFDEYKLHWETNSNYNAEELIRSLLQDDTLLAPGDSIAPDGGQPSAASRNIVAERNRRQKLNDRLYALRAIVPNITKMDKASTVKDAISYIQELQEQEGRIQGEIAQLESMASEKQDWCKKKRIAHEQQQLSSYVSGGGGSSSSPPIQVIDIKVSYVEAKIIVASLICSKRTDAIIKLCEAFESLKFKIITANITSFSATLSMTVFIEADEMEKDTIKIEIETAIRAVNHPGLQSNELLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y5R2</accession>
    <name>A0A165Y5R2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_013810</name>
        <name evidence="12" type="ORF">DCAR_0417182</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Y5R2"/>
    <dbReference type="STRING" id="79200.A0A165Y5R2"/>
    <dbReference type="Gramene" id="KZM98828">
        <property type="protein sequence ID" value="KZM98828"/>
        <property type="gene ID" value="DCAR_013810"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218514"/>
    <dbReference type="OMA" id="SENWIKF"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007869288" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM98828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG97841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="42088" checksum="838CDD314177F4D0" modified="2016-07-06" version="1">MSSTAISCLVMTLCFTLFAHSTYSQNISSFNVQKLGAKADGTLDASQFFLTAWRLACASNNSAQVYVPPGRYLISTAVVFSGYYCKRTMVMRIDGTIVAPTNYNLIANSENWIKFDNVRGLYITGGTLDAQGAALWACKASGKTCPQGATSVGFYSSRNIVVSGLRSINSQMFHMIVYKCNNTKLQGIKISAPADSPNTDGIHVQLSTGVSILSSQIGTGDDCISIGPGTVNTWIESVSCGPGHGISIGSLGWDLQEPGVQNLTVKTVTLRDTDNGLRIKTWARSSTGFVKNVLFQHIAMGNVRNPILIDQDYCPNNLNCPGQVSGVKISNVIYQDVHGTSATPVALSLQCSRDYPCSGIRLEDVVLTYNGLPTTASCSYAAGTASGVMNPTSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y6B3</accession>
    <name>A0A165Y6B3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0417195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Y6B3"/>
    <dbReference type="Gramene" id="KZM98841">
        <property type="protein sequence ID" value="KZM98841"/>
        <property type="gene ID" value="DCAR_013797"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215621"/>
    <dbReference type="OMA" id="NINEPVH"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG97854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="408" mass="45445" checksum="37DA347C7619470B" modified="2016-07-06" version="1">MGNKLGRKREAVDEKYTKPQGLYQHKDVDQKKLRKLILDSKLAPCYPGDDDHNAFDLEECPICFLYYPSLNRSRCCSKGICTECFLQMKTPNSTRPTQCPFCKTSNYAVEYRGMKTKEEKGIEQIEEQRVIEANIRMRQQELKDEEERMQKRQLVNSSSRSRSGEIEYSSIADSSSFVEGEGTNSPQYLSAMPSMRQPLPPRQNREDEFDLDLEDIMIMEAIWLSIQGNDRHGNPTSGDTAPLEQFISEDQCVAAPVETATSSSSPSGGLACAIAALAERQQGSEDSSGNYNENTSTFNTVPVCSSFSNINEPVHHQYFASSSIEVAPIIREEWGTEVGPSYAGHDAAEDMVENNNSFPEQDENGSNSQTSAAAVVPESFEEQMMLAMAVSLAEARACTSSPSVTWQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YW26</accession>
    <name>A0A165YW26_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417716</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long chain fatty alcohol + a fatty acyl-CoA = a long-chain alcohol wax ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:38443"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="ChEBI" id="CHEBI:235323"/>
            <dbReference type="EC" id="2.3.1.75"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the long-chain O-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YW26"/>
    <dbReference type="Gramene" id="KZM99313">
        <property type="protein sequence ID" value="KZM99313"/>
        <property type="gene ID" value="DCAR_013325"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219000"/>
    <dbReference type="OMA" id="VIANTRH"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047196">
        <property type="term" value="F:long-chain-alcohol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004255">
        <property type="entry name" value="O-acyltransferase_WSD1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF34">
        <property type="entry name" value="O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03007">
        <property type="entry name" value="WS_DGAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG98375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="461" mass="51113" checksum="757362E9E2393FA3" modified="2016-07-06" version="1">MELEEGEVPVSPSGQYLNSDTLSFTILAVLEFEVPVDNLNIIQLIKDMFISTNPRFSSIMIGGRNEVKRWKPVQVQVEDHVKTPEFPPGESPEVYDKCFEEYMSDLAMDPLPQTRPLWEVHVIKNETSNAAGTVILKLHHALGDGYTLIGLLISTFKKADDPSLPLTFPSLRSSSKLNGNSSSIFRVVPRILQGIINTITDFGTSILKKEDDRSPIRSANEGVEIGPRNTTTITFSLDTIKQIKSRLNVTVNDVITGIILYGSRMYMEEESFESRNANSSALVVFNTRNIGRYKSVDEMVKPNANKLWGNQFTLSHVPIPKLSHIGNSSNPLEFIREVHKTMDRKKKSSAVYMTASLIECVRKFRGPEVAAKLIHGTLKDSSVALSNLIGPVEQMALQNHPVKGFYFTVAGVPVSTGIAVVSYMGKLRVAITSEKGYIDADKFKSSILKAFNIVCKITADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZCU2</accession>
    <name>A0A165ZCU2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium uniporter protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0418367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG99021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZCU2"/>
    <dbReference type="Gramene" id="KZM99883">
        <property type="protein sequence ID" value="KZM99883"/>
        <property type="gene ID" value="DCAR_012755"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217789"/>
    <dbReference type="OMA" id="AKTNFHR"/>
    <dbReference type="OrthoDB" id="278338at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF17">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="11">
        <location>
            <begin position="153"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG99021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="333" mass="38590" checksum="B863874CC7E684C9" modified="2016-07-06" version="1">MALRRTISKRLFNKTPINFPPAPAIHQNAAKTNFKRELITSPDSATDDRGFFRRFLQRRSINQTSAKLPEFLSMPVGDKLREKLWPIHFVSSENQIRFDPPESVSGISVHEARKILRSARLEKLRSTLKQIPANSVEYSQFVKICSDVCENDEQGVECAKMLDQAGNVIVLGNIVFLHPDQVARSMEKLIYETIATPNDSRKEQLEQMEKQKAVIDRKAKSMVRAELYGGLGFMLVQTVGFMRLTFWELSWDVMEPICFFVTSLHFALAYSFFLRTSTEPTFEGFYQRRFKAKQEKLMKIHNFDVEKYNQLCQAFYPNSNVRSKFGDFGKYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZR39</accession>
    <name>A0A165ZR39_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0310272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZR39"/>
    <dbReference type="Gramene" id="KZN00324">
        <property type="protein sequence ID" value="KZN00324"/>
        <property type="gene ID" value="DCAR_009078"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213395"/>
    <dbReference type="OMA" id="KEIHDSW"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CH45"/>
    <dbReference type="Gramene" id="KZN03770">
        <property type="protein sequence ID" value="KZN03770"/>
        <property type="gene ID" value="DCAR_012526"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211956"/>
    <dbReference type="OMA" id="NYMQHQQ"/>
    <dbReference type="OrthoDB" id="10265171at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007726">
        <property type="entry name" value="SS18_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05030">
        <property type="entry name" value="SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="196" mass="20984" checksum="7A124D800C6A492B" modified="2016-07-06" version="1">MQQQLMQQQQATRGGAYNTTSITTDHIQQYLDENKSLILRILENQSSGKLGECAENQAKLQRNLMYLAAIADSQPPPSPMYSQGGMNYMQHQQSQPMVPDPAIAARPSMFYGQQHPYMALHQQQALQSQLGMSSAGSSAGHSLQSDITLGSGGSAMFPSFGRSSGEGWQAASRAAGGSKKEDNGSTGSVEDHSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CZR7</accession>
    <name>A0A166CZR7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_005375</name>
        <name evidence="4" type="ORF">DCAR_0206027</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CZR7"/>
    <dbReference type="STRING" id="79200.A0A166CZR7"/>
    <dbReference type="Gramene" id="KZN04538">
        <property type="protein sequence ID" value="KZN04538"/>
        <property type="gene ID" value="DCAR_005375"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208356"/>
    <dbReference type="OMA" id="RCWHIDP"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000778">
        <property type="entry name" value="Kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010632">
        <property type="entry name" value="DUF1221"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF969">
        <property type="entry name" value="INTEGRIN-LINKED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06760">
        <property type="entry name" value="DUF1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="241"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN04538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG86809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="683" mass="78617" checksum="555414471EFF8E1E" modified="2016-07-06" version="1">MEQFRQIGEVVGSLKALMVFREEIQINQRQCCLLLDMFTCAYETIAEEMKQHLKYEEKNTKWKVLEQPLKELHRVFKEGEVYIKQCLDTRDWWAKTVTLYQNKDCVEFHVHNLLCCIPVVIEAIEVAGDMSGCDQDEKQKKRVLYSMKYHTQNKDPKIFQWKFGKQNLVSQDFISRLETVWEEDRWILLRKIQEKGNLSSENSTKRIQRLKELLFKNLNDGGERLEGKLLPCSILVGSKDYQVRRRIGGGSQYKEIQWLGESFCLRHFFGDIEPVIPEISQELCLSHPHIMHTFCGFTDEEKKECFLVMELMTRDLSSYMKEISGAKKRVPFSLPAAVDLMLQIARGMEYLHSKQIYHGNLNPSNILVKARNPPLDGYLHAKVSGFGLSSSFSLAQKSSAAQNESNPSIWYAPEVLEEQDQADDIKSKYTEKADVYSFGMICFQLLTGKVPFEDGHLQGDKVSHNIRTGERPLFPFQSPKYVMSLTKRCWHSDPTQRPSFSSICRILRYIKRFLLMNPDHSQPEAPMPVVDYTEIDAKLRQTYAYWESSDLLPISDLPFQIFSYRIVEKEKSVSSQRETSESGSDVTSTAGDDNLILDESFSPVIERRTVISPDSLAPRPKRISSLKKIPDLKQSKSPGTPKSRSVRPPKTTSAGRALRMNSERVMSPRPRRSSGHVSDSELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ERL7</accession>
    <name>A0A166ERL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_007725</name>
        <name evidence="11" type="ORF">DCAR_0208721</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ERL7"/>
    <dbReference type="STRING" id="79200.A0A166ERL7"/>
    <dbReference type="Gramene" id="KZN06888">
        <property type="protein sequence ID" value="KZN06888"/>
        <property type="gene ID" value="DCAR_007725"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206331"/>
    <dbReference type="OMA" id="HPNGWIS"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="485"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="487"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN06888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="587" mass="65066" checksum="C53E6943FFBB9E2A" modified="2016-07-06" version="1">MDNDIFGVPADTVYQNPVAGSGNVLSSTLFSFLQSHPHDLTNQNQIMAGFPALSVMQVETPDELHDVHQLTSHAGFDTSNALVLKGPQPGRNVTGNTSICPPGSPSNKEADVRFMAGMHNSTTSLATLLAGRYGNHRNLNDIERLGYSGHHREVPEILGSNEYADLVQSSYGTSMDYNNDVMLGNVSCKWGSHKFAAPQSAGTVPQRIGLEAYQNMGNMDHNGWTSSENNIGSDNTSDSSKYSNGLSLSLLTSQPATLGGTTIVEHCSGMSASGLSCQSLHGKQLGIEQTSFNSKNMSSTFGKNPVQLSDFLSGGRFLSEMQEILAGIARYSLGNSGRSSCPTSAPVPEENFSFSFNLSTGRQKTVTGTIGNDLMTCEQEAAAKKKHLINLLEMVDNRYNQSLDEIHKVISAFHAVTELDPQIHARLALQTISLLYQSLRERISNYILSLGVHVNEGGSEEEERSFETSFIQKQWALQQLRQKDHQLWRPQRGLPERSVSVLRAWMFQNFLHPYPKDADKHLLAVKSGLTRSQVSNWFINARVRLWKPMIDEMYSEMNRRKICQNGDEINSQHKNRFNIDSQRFRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GQ38</accession>
    <name>A0A166GQ38_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0101876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GQ38"/>
    <dbReference type="Gramene" id="KZN09212">
        <property type="protein sequence ID" value="KZN09212"/>
        <property type="gene ID" value="DCAR_001868"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204628"/>
    <dbReference type="OMA" id="GNCYCKI"/>
    <dbReference type="OrthoDB" id="748166at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010410">
        <property type="entry name" value="DUF1005"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31317:SF21">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31317">
        <property type="entry name" value="OS08G0163500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06219">
        <property type="entry name" value="DUF1005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="414" mass="45375" checksum="BBC80B19CF5FAB3B" modified="2016-07-06" version="1">MDPCPFVRIVVKNLAVKYLSSSNKSDTSFYCKMKIKNLPTQISNIPIQQAQQLLDQVHASFCLNKSQFEKLNEKSNHPSFLKIKVYKRELGCGLLSESLCGYVLVDLRGVENKVSVIKDGWVEVDKNVKVCLNVRVEPDPRFVFEFDGKPECSPQVFQVNGNVRQAVFTCKFGFKNSGATNRSLRSRSSLTESGKSRSWLSGSKKEQGPKERKGWSITIHDLSGSPVATASMVTPFVPSTGTNRVSRSNPGAWLILQPGQSTWKPWGRLEAWLETGDQLGYRFEHFPDGIDAITLTNSTLSTKKTGKFTIDNSSPGPSPLSTPSSSFDSGTGSFSDGGSGSWAHLLYRGFVMSSTVTSGGKCSKPEVEVSVNHVTCTEDAAAFVALAAAMNLSMDACSSFSTKLRKELRQSFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HYN5</accession>
    <name>A0A166HYN5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_003205</name>
        <name evidence="3" type="ORF">DCAR_0103390</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HYN5"/>
    <dbReference type="STRING" id="79200.A0A166HYN5"/>
    <dbReference type="Gramene" id="KZN10549">
        <property type="protein sequence ID" value="KZN10549"/>
        <property type="gene ID" value="DCAR_003205"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195043"/>
    <dbReference type="OMA" id="KYMITET"/>
    <dbReference type="OrthoDB" id="1936278at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN10549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="146" mass="16814" checksum="2C68E1156F12F25F" modified="2016-07-06" version="1">MITPKKLLKLARKWQKQATLSRKRVTYPTARADGVHVNTSTRVEKGHFVVYSADQRRFVIPIVYLQNKIFRELLRLAEEEYGMPRDGPITLPCDEAFVDYAISLIERHATKDMEKALLISLTSDHCLSSSNIHKEHTTQQLLVCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IKA8</accession>
    <name>A0A166IKA8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_003873</name>
        <name evidence="3" type="ORF">DCAR_0104114</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IKA8"/>
    <dbReference type="STRING" id="79200.A0A166IKA8"/>
    <dbReference type="Gramene" id="KZN11217">
        <property type="protein sequence ID" value="KZN11217"/>
        <property type="gene ID" value="DCAR_003873"/>
    </dbReference>
    <dbReference type="OMA" id="FPREHRS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF7">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="64"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN11217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="257" mass="27758" checksum="1E5EAD6C037D6C8D" modified="2016-07-06" version="1">MCNSPSGVDPGRVPLEGYWNCCRLGADGWDEDRPSWFRDCRCLDVLSVIPTGNGGTIELIYMQICERSLTSLTGGPPGPPATCFVRADMLPSGCLIRPCDGGGSIINIVDHVNLDYLNFLCNDSYVLYPWSVPKVLRPLGFNDAVNGFLDDGWSILGSDGVEDVTIAINSNPGKYNNTLSILPTFGGVLCAKASMLLQHVPPALLVRFPREHRSEWADYAIDAYSAASLQSSPYAIPYARPGGFPGTQVILPLADTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YBH2</accession>
    <name>A0A175YBH2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0205747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBH2"/>
    <dbReference type="Gramene" id="KZM80954">
        <property type="protein sequence ID" value="KZM80954"/>
        <property type="gene ID" value="DCAR_031440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="108" mass="11934" checksum="337EAEB4DD03E99A" modified="2016-09-07" version="1">MVASPQNKAETLEGLEKNNEVVINEYIDSDPFFKFMDDHDDRVRPSVFTSSWDKALEAVVAHHPGMFEPSQIPSPYLLAQLLATTSSSQVPLVVDVEEEEGAEEAVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGI9</accession>
    <name>A0A175YGI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="13">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_030363</name>
        <name evidence="16" type="ORF">DCAR_0935263</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; Calvin cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGI9"/>
    <dbReference type="STRING" id="79200.A0A175YGI9"/>
    <dbReference type="Gramene" id="KZM82794">
        <property type="protein sequence ID" value="KZM82794"/>
        <property type="gene ID" value="DCAR_030363"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203025"/>
    <dbReference type="OMA" id="DAYSHDD"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000002">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="14">
        <location>
            <begin position="65"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="11">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZM82794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH15720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="399" mass="42582" checksum="15AFD5213E6F0B9F" modified="2016-09-07" version="1">MASAALFMGSSSLQVNKGFSDFTGLRSSAALPFNRRTSDDLLSVVAFQTSVIGGGNKRGVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVVAINDTGGVKQASHLLKYDSTLGIFDADVKPVGTDGISVDGKVIQVVSNRNPSNLPWGELGIDLVIEGTGVFVDREGAGKHLEAGAKKVLITAPGKGDIPTYVVGVNAELYDHSETIISNASCTTNCLAPFVKVLDQKFGIIKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLVVQVTKKTFAEEVNAAFKESSDNELKGILALCEEPLVSVDFRCSDVSSTVDASLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVASQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIH7</accession>
    <name>A0A175YIH7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_030609</name>
        <name evidence="7" type="ORF">DCAR_0935563</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="8">Necessary for the splicing of pre-mRNA. Binds to the U -enriched regions of plant introns.</text>
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    </feature>
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        <location>
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    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM84483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH11827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="506" mass="55219" checksum="639929EB23EEE294" modified="2016-09-07" version="1">MSRHDNRYNADGDADQGGSSPYDVFEDRSSHHQKQKYNEPERERLRTRERDQDRDSGRDRRHSDYHDRSGRDGRGRGRNEDSGRSRNHDYSSKRTSGFDMAPTVATTLPGAPGQQPEAPQAMPGMFQNMFPFGTAQMGAVPMMPAQAMTQQATRHARRVYVGGLPPLANEQIFCSSIVYNFALLCEILGLYGDAVVNVYINHEKKFAFVEMRTVEEASNAMALDGIMFEGVSVRVRRPTDYNPSLAAALGPSQPNPHLNLAAAGLTPGGLGGAEGPDRIFVGGLPYYFTEVQIRELLESFGPLRGFDLVKDRDTGNSKGYGFCVYEDQGATDVACAALNGLKMGDKTLTVRRATISNGQVKSEQESILAQAQQHIAIQKMALQVGGVNLPGVGTGPEEDIASPTKILCLTEVITADELMDDGEYEEILEDMKDEGGKFGDLVNIVIPRPSPSGEPIEGLGKVFLEYADTTGCSNAREALSGRKFGGNSVIAVYYPEDKFYNGDYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YRD8</accession>
    <name>A0A175YRD8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0832666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YRD8"/>
    <dbReference type="Gramene" id="KZM85668">
        <property type="protein sequence ID" value="KZM85668"/>
        <property type="gene ID" value="DCAR_026910"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197337"/>
    <dbReference type="OMA" id="INEQIVM"/>
    <dbReference type="OrthoDB" id="10248542at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000014">
        <property type="entry name" value="Proteasome subunit beta type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH13157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="235" mass="26485" checksum="F913647DC7CAE109" modified="2016-09-07" version="1">MTIMSPEASSQRTLYPYVTGTSVVAIKYKDGILMAADMGGSYGSTLRYKSVERMKAVGKHSVLGASGEISDFQEILRYLDELILHDNMWDDGNSLGPKEVHNYLTRLMYNRRNKFNPLWNSLVLGGVKNGQKYLGSVSMIGVHFEDNHVATGFGNHLARPILRDEWKEDLTFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKLTEEGMTISQPYSLKTFWGYGAFQNPTVGAEGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W1W1</accession>
    <name>A0AAF0W1W1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0100984</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W1W1"/>
    <dbReference type="KEGG" id="dcr:108199546"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010152">
        <property type="term" value="P:pollen maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080092">
        <property type="term" value="P:regulation of pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000014">
        <property type="entry name" value="MADS-box transcription factor 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="115"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 20, CHLOROPLASTIC-RELATED"/>
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        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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        <property type="entry name" value="CNMP_BINDING_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-1071">Ligand-gated ion channel</keyword>
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            <end position="85"/>
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            <begin position="106"/>
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            <end position="180"/>
        </location>
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            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="4">
        <location>
            <begin position="430"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG83404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="586" mass="67506" checksum="FE621C84C6A2ED13" modified="2024-05-29" version="1">MSAINSNLLKISQSLLQWMRHDRILDFRNRHSTNAKDSWARRSLYSLYECIPGVMNPQCKTVGILSSVFIFFFLLAFLIRTLLIFSHHAEKDRNCIVDSSHMGTALSTMIDLTKVSFILYIILMFRVAYVDPKSRVFVYDSKKVALNYLSGYFIVDLFLVVPLAQVVYFLQYMAVLCRLLSMLANQSTNALVFKSWLSKFVINLLAFFLFSHVVGSFWYYFALNRVAYCLRNACGENWCLMYVSCERRYENENFVKDSPSFKNWKNNNNATACFGPSGYKYGIYVEAVSLAQESNLPMRYIYSLFWGFQQISTLAGNQTPAFFVVEVLFTMFITATGLLLFSLLIGNIQSFLHALGRRSLEKSMRGLDVEHWMSHRRLPEKLKRQIRKSERYNWLATRGVNELLLLENLPEDLQRNIRRHLFKFVESIPIFDTMDESILDTIKERLKQKTYIKGRKLMVSGGLFDKLVFIVQGKLESIGEGSSSVVSFSEGDVCGEELVTVCLEHSIMKRNGEDFRIPAQKLLSKRTVRCLTDVEAFTLGAADLEEVFRFYSGLLIRNPHVKGDFPNRKGLPANRIKLAWSVGNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6C2</accession>
    <name>A0AAF0W6C2_DAUCS</name>
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            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="15" type="ORF">DCAR_0103201</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
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        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="293" mass="31516" checksum="775FE4700F4075D2" modified="2024-05-29" version="1">MGYLNSVVPSASQVPGQSDSPVSGGGLSQNGKFSYGYASSPGKRSSMEDFYETRIDGVNGEIIGLFGVFDGHGGARAAEYVKENLFNYLISHPKFISDTKLAIADAYNHTDSEFLKSENNQHRDAGSTASTAILVGDRLLVANVGDSRAVISRGGNAIAVSRDHKPDQTDERQRIEDAGGFVMWAGTWRVGGVLAVSRAFGDRLLKQYVVADPEIQEEKVDSSLEFLILASDGLWDVVTNEEAVGMVKPIQDPEEAAKRLMQEAYQRGSADNITTVVVRFLEDQVGTSISGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6T3</accession>
    <name>A0AAF0W6T3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF10">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="400" mass="44705" checksum="0A8B8BAF62FBA37A" modified="2024-05-29" version="1">MARNVDSTTSFDDLPLEAAEEIFTRLPVKTLIRSTSVCKTWYSNITNPTFTSAHIQHSLSCCDETAVLIIPSKYKNNKSCSLISARTGHVLKTYTIPFTTNAGMIKLVASLNGILCLAECPLDNFGRPAADEFQELYLWNPSVGKYKALFSSCFKRRGKCSYAVGLGFLEGSCDFRVVRVAFCKDDVGCLLGKVAPKVEVYSLKTNKWRRIRNPVVPRVARDSGKTVGNSMTYWLDRDPEKLYSCSEEGYIVYFDFNREVFGQIELPDDVRNCVGLMATFKLMTFESKLAVFVFNDVQESNGLKLMPSCIWLLSHEDGKFSWTPRFKVVLGEHVFPFSLSRSGALIVLSACPPVPGLVSCNLKSQDLRLTKPLVTDPCSVDTAFVESLLMLEGRDEQKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W858</accession>
    <name>A0AAF0W858_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W858"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009719">
        <property type="entry name" value="GIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445">
        <property type="entry name" value="RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445:SF3">
        <property type="entry name" value="RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06972">
        <property type="entry name" value="GIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBF-interacting protein 1 N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="820"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="837" mass="90022" checksum="7C6ABA4FD4EEB0BF" modified="2024-05-29" version="1">MSNSRGGNNGGVASIPAASRKMVMELKEIVNNSTEAEIYATLKDCHMDPNEAVNRLLSQDPFREVKSKRDKKKETKDSTESRFRSGSNTSNRGARSGTDRYGGRGGSTQFSSSDSGALHGKVGYRKENGANHYTNSSSTAPGIASRGKFQRPPAPSDFVTSENKASALSMGDGNITASQPSTGYQPAWVGGHGQKSMADIVKMGRPQSKVSSIPTPSQYSGKTSSLFSRDHGSKVSDVYTEPVSAPDEWPIIEPQQASMSVYNSHTDSQLHPDQSTMHPDDINQQTHIEPDEVEVEEDSSVENLDHVGSATVSSRNMQADNSGDASLFDNDMYKNMSSYQAHPHAFQSEEVEDIGASVPSEIANMHQLSIQDEDHEVAYEEDVPSVVIPNHLQVQSADCSHLSFGSFGSAPVTGYSDPFTSRSSRSNIEETLVETDAPVIEQSDNRNSEYYGDESIRTVADGNLVHRTSGGDAGFESQSVPQADVLKQENSEAAHTSQYAFPSSSPGYTFENAQQLNAAFNYSQTSAQMQNLTPFSNSVASLPNTLLASNIHPVRESELPYSPFPMSQSMPTKYGNTVSSIGGSSISVAEALKGFQSAQAAPQNPSGTSVATGPALPQHLVHPYSQPTLPLGPFANMISYPFLPQSYTYMPSGYQQMIAGNNTYHQSLAAVLPQYKSSVSVSNLPQSASVPSGYGAFGSSTTIPANYGMNPSAASPGASTMGYDDMLSSQYKDSNHLISLQQNDNSAMWLHGPGSRTISAVPASTYYSFQGQQGQQPGGFQQGQQPSQNYGNLGYPNFYHSQTGVSLEHQQQNPRDGAHSGSQGQPKQSQQIWQNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8I0</accession>
    <name>A0AAF0W8I0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0102840</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8I0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000028">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045774">
        <property type="entry name" value="MCCA_BT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006367">
        <property type="entry name" value="PRK08591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19331">
        <property type="entry name" value="MCCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="14">
        <location>
            <begin position="35"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="154"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="654"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG83663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="82672" checksum="DD64963B88E23433" modified="2024-05-29" version="1">MSLLAAIIRRKFNRSSNHFVIPARLYSTRSVKNVKIEKILIANRGEIACRIMRTAKRLGIRTVAVYSDADKHSLHVKSADEAIHIGPAAARLSYLKADSIIDAARRTGAQAIHPGYGFLSESSAFAQLCEDEGFTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGEQQDIELMKSEAEKIGYPILIKPTHGGGGKGMRIVTNPTEFVDSFLGAQREAAASFGVNTILLEKYITKPRHIEVQVFGDKHGNVVHLNERDCSVQRRHQKIIEEAPAPNIMRDFRSELGEAAVSAAKAVGYFNAGTVEFIVDTITGKFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRIANGEHLPMSQSEVPLLGHAFEARIYAENVPKGFLPATGVLHHYRPVTESSTVRVETGVKEGDDVSMHYDPMIAKLVVWAENRAAALVKLRDCLFKFQVAGLPTNIDFILKLANHEAFKNGDVETHFIENFRDDLFVDPNHMKLAKEAYDAAKTSAILVAACFCEKEHVSIRKSPPGGLSIWYSNPPFRVHHRATRTFELEWENDIFDKDSEILTLSIIYQHNGKYLIKTGDSSCPAQEIRVEHLGNDDFRTEVDGACINISLGAYFKDQNEHIHIWHGSHHHHFRRKIHRVLLDDDTTQWKPISGLVSHERGAVVAPMAGLVVKVLVENGMRVEEGQPILVLEAMKMEHIVKSQTAGLVSGLQVNVGQQVSDGFVLFSVKFSILYDLMSAVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDG7</accession>
    <name>A0AAF0WDG7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0206336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDG7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF118">
        <property type="entry name" value="BAHD ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG87114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="421" mass="46987" checksum="615B48B6CC9084F8" modified="2024-05-29" version="1">MQAPQIISKELIRPSSPTPNHLKNFSLSLLDQLSPNFYLPIVMFYPDMGLNASDISDLLKKSLSETLSLYYPFAGRPASKGVIECTDEGVEFFVARMDYKLAEVLDEIEPRKIDLFYPKGVLWNESYEGSLLVTQVTFFSCGALAISMCFLHKISDAGTVAIFLSDWASLARNSGLNLPPPYFISKSLVPPNDAPVVKEVTRIETLDDCVTRRFVFDASKLAELKAMVTKFGVPNPSRVEVVGAFIYKCLMAANFKAKGESSRPYLFIQPVNLRSRTTPPVPANSVGNFAWFSTVMVKNEREKELHNLVSAIKNGMAQFNDRFGKNQTSNECYAMICDMMKHMMNKGLSDECNVYKGSSLCRFPYDDIDFGWGKPIWAGLSSSVISNTFALKDAPERGIEAWITLQEEEMAFFASEVATLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFD6</accession>
    <name>A0AAF0WFD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0207353</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="116"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG88119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1685" mass="190576" checksum="00B2E1B509E061C7" modified="2024-05-29" version="1">MGCYKSMGRSIFPVYYNIEPSAVRHQTGSFDEAFREHGTQFHMDRLSKWRITLTSMAGLTGYNVHENMSQTDIINKIVDRILLEINPLTLDVAKYPVGLDPRVKALTTLLSSGTEGVIRIGIHGMGGVGKTTLAKAVYNQNIQGFQGSCFLANVREVLKRENGLVCLQQKLMDTILKRKSDKIDNVDQGIELIRARICSKKVLIVIDDLDSKRPLEFLEGPFALGSIIIITTRNEDLLDSIKVEAKYKVNELGDAESCLLFAQHAFGDIKIPDTFMELSREILGHAGGLPLALKVFGSNLLNQPVEEWSWFIDKLKRVSIEDVENKLAVSFEALNLVDPMLQNFYLDIACFFIGYKKEIVAKIMETCYTFVDHNFDILRKRCLLTTNGEDELQMHDLLRDMGKKIARNNSFGEPGKHSRLWASKDICDVLKNNKGAESLECIIPRDNQDAFDGVSFTTETFKSMNKLRFLCLEKVNLNGSFESIFEDLRWLCWDFCPLKCLPSEFNPQKLVILELPHSKMRTMWELNTVLHDFEKLKTLNMSYSLDLSTTPDFSNLPCLENLNFEYCACLEEVHVSIGSLERLVSLNLHGCVNLRSLQDSICNLRGLQCLNIGGCSRLEALPFELGNIESLTELKAWGLSVSEIPESIGRLSKLVELELNNNKSLEYLPDTICNLRSLEILDVNDCSSLEALPDWIGLLGKLVELRLSCNKNLETRPNTICNLRSLEILDISECEKLEILPDQLWKLTSLWELDARGVPLLKELPDIELSQIPLALQRLDLTGSALTALPYGTSQLTNLEDLVLKGCDHLLSITELPPNLKRIMADDCSSLERLPNLSELKQLRNLHLAYCVGLPEIVGLDELPSLRELDLGRRSSASASLGNTFTKRLFQIYCGFGHLIHICVSELPYWISQSSTLGSTFSLDVPPNVLGMILCFKHLGDWDNLHNSDPIEYSVKNTTSGFTLQGSFDNFRFDSFMVILPRSLISNSDDGDRIQLRTTNADILGIHLLYKHEIPMIDHVLGKHMTSFDELKLVTPVLQDIFLDLACFFVGRKKDEVVQILETYYCNVERNIDILKEKWLLVINDRDELRMDDLLQHMGRNIAHKNTHKEHGQQSRLWLSLDIYDVLKKQKGAEAIEGIIPRKAYYRDALMGESFTTEAFKNMCNLRFLYLKKVNLTGSFEQTFENLRWLYWEFCTLNCLPSDFDPQKLVILELPHSNMRSLWELNKVSRVFEKLKTLNMSFSQNLSSTPDFTKLPCLEILNFESCKSLEELHISIGSLVSLVCLNLNSCVKLRSLPDTICDLTALKVLNISQCSSLGALPTELGNIESLEKLFAWGVPVSVLSDSIGRLSKLVELIIRYNKNIETLPDTICNLRSLEILDISGCETLDILPDQLWKLTRLKKLYACGILLEELPDIDSNQIALSLQNLDLSNSCITAMPSGISQLLELHKLDLIGCCELMSIEELPPNLRRIIACGCISLERLPNLSNLKQLEILDLTNCSGLTEILSLEVLTSLRELYLGGCSPSLLAYTFTKSLFQIYSEFGHQIIICIWSEEFPKWISQGEYGTTMSLDLAPNMAHNYLGMILCFKCQAGGGSFLIDYSLKTSASNFIKSDTEMEVYGACMIIVPRSIFSVRDGDNRIELTAGGYAEISGLHLLYKTENCRRRKRGTNMLNSRKTWKVNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFG5</accession>
    <name>A0AAF0WFG5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0207398</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex mediates the import of some proteins while the predominant TIM9-TIM10 70 kDa complex mediates the import of much more proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion inner membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFG5"/>
    <dbReference type="KEGG" id="dcr:108206418"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000001">
        <property type="entry name" value="mitochondrial import inner membrane translocase subunit TIM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="25"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG88164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="89" mass="9585" checksum="F4AFAAF32018B249" modified="2024-05-29" version="1">MDSFSSPSASGGSSPPISQEDFMNQLKTQLAQAYAEEFLETVRGKCFDKCITKPGSSLSGGDSSCISRCVDRYMEATGIISRALFSSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGY6</accession>
    <name>A0AAF0WGY6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208417</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGY6"/>
    <dbReference type="KEGG" id="dcr:108206421"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF3">
        <property type="entry name" value="31 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="110"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="201"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="284" mass="31862" checksum="613A5F574769F8E3" modified="2024-05-29" version="1">MACMTSSLVMKPTTSTTSIFSQPIKSFLPNTCFSIQSKPIKPLLISCSAVSQFPFSLLSIEKKTHFPFFIKALLQEEYEENLDDQEWAQGDEEEEVFVDAEEEWVPSEDCKVYVGNLPYDVGSEQLAGMFQEAGVVERAEIIYDRETGQSRGFGFVSMHAVEEAETAVAMFSGYELNERLLTVNKASPKGTRPERVFRPPNRIYVGNLPWEVDNAGLKEMFSKHGKVLNARVVCDRESNRSRGFGFVTMSTTSEMNDAISNLDNQNLNGRSIIVSVAEDKKPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WH39</accession>
    <name>A0AAF0WH39_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0208807</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WH39"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF105">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00541">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjN" evidence="6">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="7">
        <location>
            <begin position="252"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG89569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113151" checksum="0DADE3D45E6354FF" modified="2024-05-29" version="1">MLIMDATINRNCEREVECEKNIKVSARWNPADPCRPTMDAAPVFYPTCEEFQDTLGYIAKIRPVAEGFGICKIVPPSSWKPPCPLKEKSIWENAKFPTRIQQVDLLQNREPMRKKRGRKRKRQRNSKIDNIRESPGIDDPSIDDLIDTEEKFGFQTGSDFTFQDFQKLAHNFKECYFGIRDTMLKDTAGQSPNGQTKRWEPSIEDIEGEYWRIIEKPTDEVEVYYGADIETAVFGSGFPKKSPSLTENISDTYVSSGWNLNNFPRLPGSVLCFEESDISGVMVPWLYVGMCFSSFCWHVEDHHLYSLNYLHWGDPKVWYGVPGTHASDLEAAMRKHLPDLFDEQPGLLHELVTQMSPSILMSEGVPVHRVVQHSGEFVLTFPRAYHSGFNCGFNCAEAVNVAPIDWLQHGQSAVEAYSEQHRKTSLSHDKLLFESAREAIRALWELSIMNTENPKNMRWKSVCGKDGLLTKAIKARVEAEKKRIEHLPLHLKPQKMEKDFDLTCERECFSCFYDLHMSVACCKCSPDQFACLRHATILCSCEKNDVFVVVRYTMDELSTLIKALEDEAGALKAWPSVDEGLVCINCEDSISGISSAKQNDDPAKFPKRDETLQEDTADGCMSQVVTNKLKEDVVGKSNDTMTINQSYTVISAVVERMLNHKSDVCYDNMMANEGDACNNVIREAFHKADSRQKLDSLQLGGCPGSSVSYVLPKNVCPSPSMDVADRGASNNDLTFGCMSSNEVVDQLGIKDTHSVRNGSLQKFSFDVHPINIGNAVSGRLWCNKQVIYPKGFKSYVKFFNVLNPTQISGYISEVVDGGLIGPLFKVTLEESPSKSFVDTSAQKCWEMVIDELNQELSRCQSIGKAVFPSLDPQSINGLAMFGFLSPHIIKEIEALDPNHHCSEYWNYKLPMKEKLTDNTYELPDAKKFNYHTGSNLSGGNTTPLTNTEISIGGQAHSINKECMPVLERLMQNANPEELNIVQRVFCVDSQTAEWKAALAKLNEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHV4</accession>
    <name>A0AAF0WHV4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-aminoacylpeptide hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0207743</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHV4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF274">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:GPI anchor biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG90093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="52731" checksum="96B67062C3BAFDFE" modified="2024-05-29" version="1">MENPVIKPLSSPSLNPNKLLKEQFVSNLNGCSMFEIFVLTSMLSVAILIRRCIGFHGTTNHGDTDSSLKKNDDAIVATKKWSSYMVTTAVDFVFVVIPLIIFPTVLADWTYINAILLLTVLLLCFTYRRCVSSVSLEGVQPVRTYISSYRVTMMLVTCFCILAVDFNIFPRRYAKTETYGTSVMDLGVGAYVLANSLVSRQARGIMKTSLRNALQSASPLLFLGFARIAFTSGVDYQVHVGEYGVHWNFFFTLAAVSILTSVVNIPPNYCGILGSLILIGYQVFLVRGLNLYLLSEERGSDIISQNKEGIYSIFGYWGLYLVGVQLGNFLFFGNHFSATITNNWSRIRVWILAILFWLLTLFLNQYVEKASRRMCNLAYVTYVLAANLQFLAMAMLADYIPGPKISILEEAFSKNLLGTFLLANLMTGLVNLSIDTIYVSPLPALIILSIYSLILSVAVVIADFYGLKLKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLQ8</accession>
    <name>A0AAF0WLQ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0309807</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF851">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE SD1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="180" mass="20251" checksum="7AAB51610D8DD726" modified="2024-05-29" version="1">MNPKISDFGMARSFGDSETESNTGRVVGTYGYMSPEYAIEGAFSVKSDVYSFGVLLIEIVTGKKCRFFSHPDHNLNLIGHAWRSYKEDRLLELIDESILESSVQSEVFRIIVIGLLCVQQYPEDRPTMSSVLMMLTSKASLPQPKQPGFFTERRLDETDFSGSMPYSSTANQSITIVRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQX0</accession>
    <name>A0AAF0WQX0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-carboxy-D-arabinitol-1-phosphatase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0313788</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-carboxy-D-arabinitol 1-phosphate + H2O = 2-carboxy-D-arabinitol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17837"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58008"/>
            <dbReference type="ChEBI" id="CHEBI:58185"/>
            <dbReference type="EC" id="3.1.3.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.63" evidence="8"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WQX0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047538">
        <property type="term" value="F:2-carboxy-D-arabinitol-1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000018">
        <property type="entry name" value="Phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF10">
        <property type="entry name" value="2-CARBOXY-D-ARABINITOL-1-PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="77"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG94492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="509" mass="56424" checksum="214012A603BB69A8" modified="2024-05-29" version="1">MFSITPSITTHNPSLHFHTSHRNCFLLNPKKLFSIRSLSSSLQVPINEENEENLSPELRYSRPFPAVKSAKRVVLVRHGQSTWNAEGRIQGSSNFSVLTQKGEAQAETSRQMLVDDSFDICFASPLNRSKRTAEIIWGTRKEEILTDLDLREIDLYSFQGLLKHEGKEKFGEAYQEWQKDPANFCIDGNYPVRQLWERARNCWDKILLHDSRSVLVVAHNAVNQALIATAMGLGTEYFRVLLQSNCGVSVLDFTPRLDDLPPYVCLNRLNQTPGSPVAGGDSGGRKASKQIILVCHASSQSKFKASVPLVEDSPMDMLGIIQSQKTAELLLDLRVKTVVSSRNLASIATANTIAQVQEAAECLGANCVPRLVEKKQMQNLGVDNILQLSQKGVNDAPHSPGWLKILDDEVITALWDQSKNAWESLLDELSNQPENEEIIVAVADSSANVALLCHCLNVTKEWIGSFHLDAGSISVIDFPDGPAGRGVIRCINYTAHLGRWSIPITTSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WSP2</accession>
    <name>A0AAF0WSP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0314339</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WSP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041768046" description="Xyloglucan endotransglucosylase/hydrolase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="18"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG95037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="33473" checksum="5BEBA0D8EEB69B86" modified="2024-05-29" version="1">MASHFLEIFGFSLLVFVSFVSGGPADDVSFDQNYFSLWGLNHITRVDNDKEVQLLLDQYSGGAGFRSISEYGSGKFGIRMKLPDANSTSGIIICFYVCFYSSDIILTSAPDSSNPGNHDELDFEFAGGGLQTNIFAGDSGSREERYQLWFDPRKDFHLYEILWNPYMVVFYVDKIPVRVYKNYTDKGVNYISNPMHTEASVWTGDWAGTVDWKQGPFVSSYRRFGIHGCKSQNTSMNQECLSPNLHWNLQKDLTPHEQKMHQIFREKHVVYDYCLDKERQQNHPECQLPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTD4</accession>
    <name>A0AAF0WTD4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phytol kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0415079</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTD4"/>
    <dbReference type="KEGG" id="dcr:108215602"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039606">
        <property type="entry name" value="Phytol/farnesol_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523:SF7">
        <property type="entry name" value="FARNESOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523">
        <property type="entry name" value="PHYTOL KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG95752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="32336" checksum="A75849A0D8FB7EB1" modified="2024-05-29" version="1">MALISTSSTNLAPTLSRSHRCPHLSTTSCAPSSFSILVCQPFVSTPILSRPTKKKVEKAGAIMFSDSPLAADLCATAISGVVAVSVLKLWEETAKRGVFDQKLNRKLVHISIGLVFMLCWPLFSSGHQGALFAALIPGLNIIKMLLMGLGILKDEATVKSMSRFGDYRELLKGPLYYAATITLACAVYWRGSPIAIAAICNLCAGDGLADIIGRRFGSQKLPHNSNKSLAGSVAMLIGGFIASVGYMHYFSSFGYLEETWGMVLGFLVVSLAATLVESHPLSTVLDDNLTVPMATLLVGSFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUZ0</accession>
    <name>A0AAF0WUZ0_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0415911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUZ0"/>
    <dbReference type="KEGG" id="dcr:108216048"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711:SF1">
        <property type="entry name" value="ECTODERMAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG96575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="168" mass="18585" checksum="FB019CD612309243" modified="2024-05-29" version="1">MEMVALRTEAVAVKKLIVSTFVVETVLAAQRSVLCLLLLADVLLKIKDRLLDLIGGDQRFPVEELHRRKRPETENVDGSDTEDDEEDDDDVEQDDDEGDEDFSGEEGGDDDEEGDPEEDPVANGNGGSGDEDDEDEDGDGDEDEDGEDEEDDEEDEDEEDAPPAKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WV92</accession>
    <name>A0AAF0WV92_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0414870</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ARPC5 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG95546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000002">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
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    </dbReference>
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        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="132" mass="14978" checksum="C689AF235931B4CF" modified="2024-05-29" version="1">MAEHVEADNAEAIITRIEHKSRKIESLLKQYKPVEALKTALEGAPPKTRDERCKSANWIVVHRALMAIKDVDGMFSSLDPEYYDILMKYLYRGLSTGDRPTCDQCLRIHEKLTQKAGLGCILRCLTDTINTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WV98</accession>
    <name>A0AAF0WV98_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0415767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WV98"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF10">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="15"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="18381" checksum="7D2421151AFDF6A9" modified="2024-05-29" version="1">MAEPEKRFPLNAQDYVLYEEVGEGVSATVFRALCVLFDEIVAIKVLDLEKWNNDLDGIHREVQTMTSINHPNLIWAHCSFTSGHNLWVVMTYMVRGSCLHIMKSAFPEGLEEPVIATLLREVLKALVYFHAHGHIHRDVKVTSPLLVVCRSKLHYFSHWI</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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            <dbReference type="EMBL" id="WOG95558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="80" mass="8653" checksum="E83A1E95057F416D" modified="2024-05-29" version="1">MKENALSKLVSGGGVNVSEYGPDCTPVIVYNFLYVLPKLVSGGGVNLGEYGRDRTHVIVYNFLYETLSFPALALDPKATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WZB8_DAUCS</name>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417762</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108217668"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TBL"/>
    </dbReference>
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        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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            <begin position="23"/>
            <end position="345"/>
        </location>
    </feature>
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            <end position="81"/>
        </location>
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            <begin position="82"/>
            <end position="340"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG98419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39023" checksum="9C4F8479C0961BE8" modified="2024-05-29" version="1">MASFSISAIVVLTILAAKEIEGGGGAGTCNIYEGNWIYDPSYPLYNPADCPFLESREFDCQKNGRPDKNYLHYRWQPTGCNLPRFDAHDFLQRFRGKQLMFVGDSLSLNQWQSLTCMIHTSLPQAPYISNKIGGLSNFTFPDYKVSFLFSRNAYLVDIRSEIIGRVMYIESVEYSRGLWEGMDFLIFDSWHWWTHTGSDQLWDFIREGNATYKDMNRLVAYEKAINTWAKWANSRADPAKNRLIFQGVSPDHASPPKTCVGAEAPLPGPSPKGTNHPAEAVLEKVLSGAKEVYLLDITGLSELRIDAHPSAYGFGGSKFPDCSHWCLPGLPDVWNQLLYTYLFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3T5</accession>
    <name>A0AAF0X3T5_DAUCS</name>
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            <fullName evidence="2">cytochrome-b5 reductase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DCAR_0519383</name>
    </gene>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3T5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidoreductase FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="128"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH00027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="23752" checksum="E631AF5B769833BE" modified="2024-05-29" version="1">MQLFGLSETQIVGVAIAAFVLAVTVAIVFSSKKSKGSTITNKIDIHMMSKPKPSSVLGLPFRELISCSLLNRKSQLSLDSDVGHFKLVITVMSHHFHVMRVDDYMSVKRPKGRLKYQPGQVSVRALGMLAGGSGITPMFQVARAVLENLNDTTKVHLIYANVCLEDMLLKEEIDRLASNYPDHFKVYSCGSPLMNKAMDANLEALGYAPETQFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X6C5</accession>
    <name>A0AAF0X6C5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_0521637</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X6C5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF283">
        <property type="entry name" value="OS02G0633066 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042063937" description="Protein kinase domain-containing protein" evidence="21">
        <location>
            <begin position="23"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="305"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="421"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOH02248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="26">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="83500" checksum="B0EF8778D79C4081" modified="2024-05-29" version="1">MQTLVAYGFVTMLVLLARPATTSDIKNAIAKARVITKPGCKSKCGNLTVPYPFGIGVGSGCSINSSTWFDVNCNTSFNPPRAFISDGNIQIFDISDTQMRVANVLATSCYTSTGELTVNRYAYTNLLDTPFSFSNLNTFTVVGCDALALIKGSEQRNFSGGCISVCSKPEDILDYGCSGIGCCQTAIPKGLSVYSTILASLRNHTRVWSFNPCSYAFLGEKDSFKFRGASDLSDPTFLNRTVDSVRIVLDWVIGNQTCDEARKSNVYACQSNTYCTNSDNGFSGYFCSCLKGFEGNPYLSPGCQDIDECAHPNKNLCEKICINTRGSYNCSCPPGYHGNGKKNSQGCIAENSKFPVIKFSLVSFLYSTIKRRKLIKEREKFFQQNGGVLLKQQITSSGSGGVESSTRIFTAEELEKATDHYATDRIVGRGGYGTVYKGTLGDDRVVAIKKSRIIDESQIEQFINEVVILTQVNHRHVVKLLGCCLETEVPLLVYEFVANGTLFQHIHNSRGVMWLSLENRLRIATESAGALAYLHSAASMPVIHRDVKSANILLNENYTTKISDFGASRLVPLDQTQVTTLVQGTLGYLDPEYFHTSQLTDKSDVYSFGVVLAELLTGRKPLMLQSPAEEKNLATYFIMSVKENRLFQILEPRVVREGSLDQLEAAGKLVKRCLSLNGEDRPTMKEVAMELEGIRKYTKHPWANNNHQQALMIHDAEESTDLYTVSLDTYSSIRDFSGQDSVMDTKHLLFTANGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X883</accession>
    <name>A0AAF0X883_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0521066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X883"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042096173" description="Cytochrome P450" evidence="8">
        <location>
            <begin position="23"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH01681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="58226" checksum="018F8088E888B459" modified="2024-05-29" version="1">MILAFLPLSIVIFLYFCKICTSNAPLLTDWPLVGMTPGMLKNVHRLHDFLTCLLQQSHHNFQFHGPFLANMDVLFTCDPANVNYILSKNFTNYPKGPEFGKIFDILGEGIFSSDGHLWEVQRRTTMSIFNHAQFYDMLQKTVGEKIEKALIPLLDHVSKHGIEVDLQDIFQRLAFDNICSLVLDHDLQSLSLDLPYIPYQKVVPDVEEAMLYRHFVPESIWKLQRWIKSGKEKTLSKARETIDEFLYTCISMKRKQVALKNSEHISAELDLSAAYMELAYKENPENPDKFFRDALVNIMVGARDTTSTALSWFFWLLWKNPRTESKILQEIKTNFNTEDLNNCNLFKKKEEMHKLVYLHAALCESLRLFPPVPLNHKAPKQPDILPSGHRVGKNSKILLSFYSMGRMEDIWGKDCLEFKPERWITEKGDIKHEPSYKFTAFNAGPRACLGKDMAFTQMKILATTIIHRYSIQVVAGHPIVPKGSVILEMKYGFRIRVEKRSEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8E8</accession>
    <name>A0AAF0X8E8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Alpha-galactosidase</fullName>
            <ecNumber evidence="3 8">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0622772</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3 8"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8E8"/>
    <dbReference type="KEGG" id="dcr:108224678"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000008">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000093">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF33">
        <property type="entry name" value="ALPHA-GALACTOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042254609" description="Alpha-galactosidase" evidence="9">
        <location>
            <begin position="31"/>
            <end position="421"/>
        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="421" mass="47192" checksum="16453A37FB1DD1DC" modified="2024-05-29" version="1">MGFYCRFSCRLLVSLVCFWLFVTSYSGIEAAEEIKVERTADDQLNGFVLDNLQEIRMKLVANGIGLTPQMGWNSWNHFQCNINEQMIRETADAMVSTGLAAAGYKYVNLDDCWAELNRDSQGNMVPKRSTFPSGIKALADYVHSKGLKLGIYSDAGVRTCSNLMPGSLGHEEQDAKTFASWGIDYLKYDNCFNNNVTARQRYPIMSKALQNCGRPIFFSLCEWGQEDVATWAPGIANSWRTTGDIVDNWNSMSSLADQNNQWASYAGPGGWNDPDMLEVGNGGMSVEEYRSHFSIWAIAKAPLLIGCDIRAMNNATHAILSNKEVIAVNQDKLGIQGKKIKSNANLEIWAGKLSKNRIAVVLWNRGASTAWITAYWPELGLAPYTEVNARDLWRHQTYKVKGKIYRKVKPHDCKMYVLSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9E5</accession>
    <name>A0AAF0X9E5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0623072</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="95"/>
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            <begin position="1"/>
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        </location>
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            <begin position="298"/>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03673.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="332" mass="37085" checksum="56406F14F08B7351" modified="2024-05-29" version="1">MDEFNFSQSMEDPTQSLSEFNPRPISTRLHPDSRAEADPGECNVARKVQKADREKLRRDRLNEQFTELGNTLDPDRPKNDKASILGDTIQVLRDLTTQVDKLKAEYATLTEESRELTQEKNDLKEEKASLKSDIDNLNLQYQQRIRAMYPWGGMDPSVVMHPPSYPYPVPMPMAPGPIPMHPSIQPYHYFGNQNPALVPNPCSTFIPYLTPNTIIEQQSRTVNPVVQASSRSRISSKQDSRQRSSDHGESKAVEDSDDVATDLELKTPGSTADQDSSSRQQKPKKSSRKDRTLSDGNSSSRCSSSHSVLVSSSDSVFGAGIGSDDTEKEQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR000387">
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    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="46"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="106"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH05784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29898" checksum="114D19D4063D08B2" modified="2024-05-29" version="1">MRKRERENPCGICGHYHKYEEGEVCGICGHRMPDDALKSSIQVSAFPSEILPEFLYLGSYDNASRSELLKTQGISHVLNTVPACQNLYKNSFTYYCLQGDQDIPFDDAMHFLEQCEKDRARVLVHCMSGKNRSPAVVVAYLMKSKGWRLGQSYQWVKERRPSVEISQAVYQQLCNFELKIFGDVESTNPTLPVFSASGAPAFSFGFPKPNEAAAIPAFSNTGTTSIFNRSTLDIPPQEFTFGAAQIQNTSGVPLGANPPNANGSDVSMDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFZ3</accession>
    <name>A0AAF0XFZ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0626504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFZ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000001">
        <property type="entry name" value="Magnesium transporter MRS2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000023">
        <property type="entry name" value="Magnesium transporter MRS2-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF29">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH07075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="367" mass="41203" checksum="29D4833F87313B0F" modified="2024-05-29" version="1">MVYPPEDLNRAAVHAAPARRKGIGTRVWLVVSESGKSHVEEVGKHSIMRRTGLPARDLRVLDPMLSYPSTILGRERAIVVNLEHIKAIITAAEILMINSTNPLVVQFVIDLQDRVSTPSDRSNSPPEILPFEFKALEVCLESACRCLESETQTLEDEAYPALDELTSQISTLNLERVRQIKSRLVAISGRVQKVRDELEHLLDDDNDMAEMYLTEKLVESSREQASLREESGIEEVQVDDQRYEESEAEHSENNSDISTVFKPNIEELESLLEAYFAQIDGISQKLANMNEYVDDTEDFINIMLDDNTGNMILNAGIVVVGLFGMNIHIELYDGQPKQFWETVSGTVGGCLALYLIAIGWGKKRNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIM4</accession>
    <name>A0AAF0XIM4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Helicase MOV-10-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0726924</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIM4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049080">
        <property type="entry name" value="MOV-10-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418">
        <property type="entry name" value="CANCER/TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418:SF1">
        <property type="entry name" value="CANCER_TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21634">
        <property type="entry name" value="MOV-10_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase MOV-10-like beta-barrel" evidence="8">
        <location>
            <begin position="239"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="595" mass="67317" checksum="E43815CF435234AA" modified="2024-05-29" version="1">MLSFLRCVLCCDDEINTGQSESESYYDESPSQPFNYRVILDNYDTFSRTQIDKTTRTHDICTTYRFPPKSNPPPVYNPPKQTLSRINDKVISESKKTPTGKDTIVFPRPASSSSRVVLPPQPSPISPSLSKKPILAPAPSSSTLNQAKSQYNVVEQGSTPLFVIPEDIKGLIRKDIVPGVLKKPLSPQTYKDYFEALLYAEDYYLEKWDGFEMTNVTLKLNEAEIHRRKGKSKNKNNNQKDEKIFVEFEVDSIPERRPFLLSRDFASVRPTGTKVDPFQGIIFRVVRSNIVLVEFGEDFHAQHYSSCKYDVKFSFNRVCLKRAHQAIAAVSDMFFRKFLFPDCRPEKVLLKGKILFSSHKLDSSAISRIINLSGPPAYLLEGPISVTRDKQLSRTGMVIRDAVVQLCQVSSHNRILICAPTNSTCDVFMRSLRKEVGDSDIFRANAAFRELDGVPIDILPFCPYKKKEEVFSCPSLSQLQKFKVILSTFMSSFRLHNEGIEGGHFTHIFLVDASSAIEPEVLVPLTNLTNASTNVVITGAPENQSGWVRSTIARQNGLKTSYFERLRQSKLYRDLNPESITQISDDSSSSLSYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJM2</accession>
    <name>A0AAF0XJM2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8039 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0727084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="60" mass="6538" checksum="B32F19FA649C3541" modified="2024-05-29" version="1">MADWTGPATRLWDRYYLNVGWLGLLSVARLGGQRALGCGAAIITQLRLPCLACQDLCFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTX6</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0933755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPR1-interactor family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH14238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTX6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010112">
        <property type="term" value="P:regulation of systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NPR1/NH1-interacting"/>
    </dbReference>
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        <property type="entry name" value="NRR REPRESSOR HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15699">
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH14238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="107" mass="12226" checksum="777913BE1221AEE7" modified="2024-05-29" version="1">MDKGKEGKKRKVENDGGETEDEKMEKFFALIKSTRQRSSGEGQRRKAEDQYGWCPKFQLEDFEINKFISVPAPADNITGPSTTAEDDQNKQEIVEEENDILNLKLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XVL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0934338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVL2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF51">
        <property type="entry name" value="OS06G0556300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="16"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="613"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="697" mass="79598" checksum="83C762A76D3BE26E" modified="2024-05-29" version="1">MGSQVKKKLRIYGDEEDKISSLPDELIHHILSFTDAKEAVQTSVLSNRWKSLWITLPFLNFGEYRYSSPKNNTKFIRHVLSKRNRQSDLFELNFCVYNKGLRRCLIENVVEYAISHNVQSLDFGLLYKHQPFKLSTFNSNSLKKLTLRVRLEEFAMQLGCWDLPCLTTLFLKYPDFDLTKGNQISDSWFTCLPSLRDLGLENWDLSISSFSFSIPDLTTLRLSNCILPKMVLDLPSLITLDLHNVNLPYDMSDMISTLINLQNLTISSPFTQDHVISCPRPLLNLNIRTSSSRYDSGSGYIIVFAPKICNFTSYGIFATTFGVPELETVNIRLQGWFQGLQSSKEEIYHRLTNMFPGLGNAKTLTFDLESIKALNEISSLLVWLPSPFSNMKYIKIPKTYKQSNMSSALRSYLLGGSPRSTIVTTLPQGFRKWRKCVMHIYISCCLQNMLPQTIAVSMTKQNEEHVMEDPVVDSDRVRCSDAPLERTKKDQASSSQGDKDSGLWRGHEVNPEFVCLLDRIMHTYPETFEHFTTKNKMLSAMNLNLLCTSLDNFSKVSLTEVDCEMIDGYRDICAYLQIQGFDVSWVVNRLNYIEHLCFSQPLIPELHGIDCCIEGAKTEVQNMQAHVDDAKMNIQGLQARGKINVQDLQARVDYSKTKLQELQNLRRKKMTEIEKAFGTRGTDLAVGLIGDYLLSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWC2</accession>
    <name>A0AAF0XWC2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934963</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWC2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
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        <location>
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            <end position="328"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41880" checksum="DF21EA1B297793BC" modified="2024-05-29" version="1">MIDLCGKVRAFDVAWGLIDDMKENGIEVSNSTFSVLIRRYVRAGLASEAILAFNRMGEYGCVADKIAFTNVVSVLCKKRRAVETQEFFDGLKGKFEADVVVYTSLVNGWCRAENISEPNVYTYSIVIDALCRCGQITRAHDVFAEMIDVGCQPNSITFNNLMRVHVKAGRTEKVLQVYNQMRKLSCAADTITYNYLIESHCRDENRDEAVKVINTMVRKGCEANASTFNPIFRCILKAGDVNSAHRLFARMKEIKCKPNTVTYNILMRMFADSKSADMVIKLKEEMDEGEIEPHVNTYRILISLYCGMGHWNNAYKFFREMIEEKCLKPSEAVYEMVLQQLRKAGQIKKHEELVEKMVIRGFVARPL</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
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    </reference>
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        <subcellularLocation>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </dbReference>
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    </dbReference>
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            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANG4</accession>
    <name>A0AAF1ANG4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0205811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANG4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="34000" checksum="6766BB7A26FD6268" modified="2024-05-29" version="1">MLTQQYEGNRVDGTFTLTAYTNMVNDLNGKIKNVTLTKSHLKNRLRTLKDHFSQCYDLFRGVGLSGFAWDPVTKLFTADEDARPDAVKWKTKKVFYYDKLSELFAKDRATGGKAETAKEKCKKWASGSELGVETIDEIDELLNANEVTLENFNTEDDLQVLSGTAFPQEQPSNSSKSKCMKRKYEGEDEMENSKGKKKKLVVEDEMAKIMSSMEHVADALKEGNVILKETNLILEQSRQRVYSEEEIFKQLEVIGLDPEAIYSAYLFLAKNQNEARILFGCPLPMRKRLLEDLMRQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARF2</accession>
    <name>A0AAF1ARF2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARF2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF182">
        <property type="entry name" value="OS09G0381850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="130" mass="14488" checksum="B3A2FD9777DC880F" modified="2024-05-29" version="1">MAEHIETPQGINDYESFIRESGGILEPTKSELEEYLSEKIVAPTSKFDVLAWWKGNSSKFPILSKMAADILSIPISTVASESTFSAGSRVIEPHRSCLKPEIVEVLLCGADWVRELYGLKKAKQVNGYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARR7</accession>
    <name>A0AAF1ARR7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0312095</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This protein functions as a defense against chitin containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARR7"/>
    <dbReference type="KEGG" id="dcr:108215416"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF22">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042021306" description="chitinase" evidence="15">
        <location>
            <begin position="26"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="16">
        <location>
            <begin position="26"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG92818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="31697" checksum="4C1C52EB5E40C4C2" modified="2024-05-29" version="1">MAKISSLLFLLVLTLSSALFYTSQGAGIAIYWGQSGNEGTLASTCASGNYKYVLIAFLTTFGSGRTPVLNLAGHCTPSSNGCRVLSNVISTCQRSGIKVLLSLGGGSNSATSMSSPADARQVATYLYNNFLGGQSASRPFGSAILDGIDFDIEQGSSLYYDDLARALSGFSTPQRKVYLSAAPQCPIPDAKLDAAIKTGLFDYVWIQFYNNPQCHYTGTATNLLARWRQWAAALPSRSQIFLGLPAASAAAPSGGYISPSALISQVLPTIKRTPKYGGIMLWNKLYDKTFSSAIKNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW83</accession>
    <name>A0AAF1AW83_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0414781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3F7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF44">
        <property type="entry name" value="REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="61585" checksum="E9B114F5A33F03A0" modified="2024-05-29" version="1">MLDVIFGWGKASKCKKLLRRVQCRLKLLKNKRSSILRYSRDDVALLLKFGYDQNAFNRVEHIIKDERMFAVYDLLEAFCEFIVINLPYIRKHRDCPNDINEAVSTLVFASARCGDLPELCKLRSLFAKRYGQKMTKIALELLPGNLVNQTIKENLSAKSVSDDARYKLMDEIASSIKEEPLALEYTPDQSSDSETIPESGTYGKHSEYGDSDLQAMIVSKAEGNEEGNRSPLALDYSKQPFLTERREEGGIYEDSTSEMLSDQKPEKIVYLDDIEEFKFPVRKDGNNIQDQRLFLFKSSDSTRKESEVWYDGDIDLHSSMNKKASSKSYIKSRKGDGNKLRKRTQSAENINVNDLESSIYYGGSMNSNLKSYECRDLPKKVQVKDGSDDSESPPLLPKRRIVAVEDSHGVKNKPGSCNGVTTTQPESVHKEKMYVDYLPYEHMSQAKQEAECISTLIRPKQTQHSYTRATTMPPKRPEDDQADSILRSKSFPFQQPTDLWSNAPRSPCIHPKLPDYNELAEQFMALKKAALQKQPQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B513</accession>
    <name>A0AAF1B513_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0626303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B513"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010928">
        <property type="term" value="P:regulation of auxin mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000142">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000058">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000081">
        <property type="entry name" value="Octicosapeptide/phox/Bem1p domain kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF792">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="722"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="668"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="753"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH06874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1006" mass="112574" checksum="CD9F23B173B959D0" modified="2024-05-29" version="1">MASEVPGTSGEKSKEDVCFYRDNRGLLNISVQTGEEFSPEFLRDRVAAQKCGGCDMDHYQQKGGMNLDNDPHLVYIRKGGSIEALIQRSLSNGSMYDRATESPRSPGNKVNSPFSYQYSSPPHKSGSPKSHQLTFGYNSGVKEGYPLSAKMKFLCSYGGRILPRPNDGKLRYAGGDTRIISIRKSVTYRELMRKTLAICNQPHTIKYQLPGEDLDALISVSSDEDLHLMIDEYHDLQKSSQRLRIFLVSTNDSENSCLLGTRKVQQSDADYQYVIALNGMQDQNLNKSSSTESLSSCWGGNSPKYERDSPATFHPREIKDSGSPVKGNMMLSNLNPQVCSSPRVGTKRLVHSPSSPACLQQMKVLINNSPMKIHEVGTLKDGCEGNTLCASKSRANENIYWHHQDRNMGGSSNKDVTPLIELESFSPLYDQGRDMEAEKLKFKISAPGNVPYQNATDLLSDYSLPQSYHRTISQLELQQQNRPNYYCKEELVLDKDFINLEASDTRKTCNDCDQDLINFMTDDDSLLGQNQNTAERNASKGEQVFGIEPKLSNRHCSTNLRIDLLKSSRDGSCIRKILDDNDQIVTNLSELSLVKDPVFAEVAYSYDDVVNDRQHDAEIKDSTTLVDSVHSKDHDESKMSGLSVIVEDVTDNMPPDMSLTPRVVQFVQDEPSESDTESDPLNLDTKCKDPTGVDVGNSISDTVIAEMEAGIYNLQIIRNSDLEELQELGSGTYGTVYHGKWRGTDVAIKRIKKSCFAGRSSEQERLIKDFWREAHILSKLHHPNVVAFYGVVPDGPEGTLATVTEFMVNGSLRHVLLKKERALDRRKRLMIARDAAFGMEYLHMKNIVHFDLKCDNLLVNLGDPKRPICKVGDFGLSRIKRNTLVSGGVRGTLPWMAPELLDGSSSRVSEKVDVYSFGIAMWEMLAGDEPYANMHCGAIIGGIVNNTLRPPIPAHCDAEWRKLMEECWSPDPAVRPSFTEITNRLQVMSMAVQPKQRIKVKRSIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8F8</accession>
    <name>A0AAF1B8F8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-specific transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0626605</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
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</entry>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAH5"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="443"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="887"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="30"/>
            <end position="43"/>
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            <begin position="518"/>
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    </feature>
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        <location>
            <begin position="897"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAZ1</accession>
    <name>A0AAF1BAZ1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0830708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF73">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR_1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
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        <location>
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            <end position="227"/>
        </location>
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            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
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            <end position="441"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH11228.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="52607" checksum="0162D5A895DA67BC" modified="2024-05-29" version="1">MSRSITSSYRFTNPIFVFSRTQFSSSSADQYWAQLQKNGSNVERNLNMIRAQLDTSSVTQVLQRCVNQPVLGLRFFIWAALQPNYRHTTYMYNKVCNLLKIKLNPCVINDVMEDFGSEGCLGSVKRFKVVLNLCREAKLANEALWVLRKMEEGGCRPDTLAYNVVIRLFCDKGDMDEADRLMREMKGFGLYPDMITYVAMTKGFVEGDRLEEACGLFKVMESHGCLPNTVMYSTLLDGVCRFGSLDKALQLLGEMEKEGGASKPNVITYTSVIQSYCENGKSLEALSILDRMVANGSVPNRVTASTLINGLCLEGHVNEVYKLICRFVGEGFVSDTECYSALVVSLMRVEKIPEAEKVFRKMLATSVSPNSLASSLLIKKLCSKGQILDGYRLYVEIEKLGCILTIDSDIYSILLAGLCQDGHLLEAANLAGVMVEKGIGLKPPYAEIAIDHLKNLGELELVSHILRLQNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD43</accession>
    <name>A0AAF1BD43_DAUCS</name>
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        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0934841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BD43"/>
    <dbReference type="KEGG" id="dcr:108192661"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042046337" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="70"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH15304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="540" mass="60749" checksum="7206FADB7E67F3F8" modified="2024-05-29" version="1">MKISLAKIFVFCSFWVILSSASSHEDFVQCLTRFSPNTNSITKQIYTPQNSSYTSVLESSINNLRFAGSSTPKPLVIVTPTEDSQVQAVIYCAKKHHIQMRIRGGGHDYEGLSYVSKAPFVVLDMVRYNKIDVDHEAATAWVQSGATLGELYYSIAQKSSTLGFPGGFWYTVGVTGLIGGGGYGNLRRKYGLAADNVIDARIVDVKGRILDRNSMGEDLFWAIRGGGASSFGVILSWKLKLVSVPEIVTAIQLDRTIEQNGTEILHKWQSVAPNLPKDVEIRVIAATIWKNRPNPVARTVLTDDSIARNRAEKTILLRFSGYFLGGQEKLVSMMNKEFSELGLTKEDCTELSYIQSAQRFSLLSTSESPEVLLNRTSFRIPFKAKSSYAERPISRKGLEGIWTRLLEYDPGTTNFVFTSYGGRMNEIAESAIPFPHRAGTLFMIYMRVQTDGDAEKRIEWIRELYEYLTPYVTKNPRTSYVNYNDLDLGVNNEEGPTSYKRASVWGKKYFKNNFDRLVRVKSSVDPGNFFRHEQSIPPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDB7</accession>
    <name>A0AAF1BDB7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0934797</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDB7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000144">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF347">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="419"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="665"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH15260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="787" mass="88998" checksum="AF78A6CC757D8300" modified="2024-05-29" version="1">MNKNSSICKMQFSPKNTNTISKIMWGIALGSRVKRIIPFHSNHLRHYSSSHSQLPNYVHANLLKNGTFGELSVTNYVLSLYIKSKDLVCAVQMFDEMLDRDVRSWTMIISGLVQVGSCRMALDMFADMHKEGIVPNQFTFSSVLKCCSRLNEVRMGKSVHGWIVCNGVFVDTALENSVLDFYVKCGSFDSAKLYIESMTNKDIVSWNIMINAYIQIGDMETALGLFRRLPVKDASSWNTIIRGHLQNDNYGIAMELLYEMVKSGTALSKETFSIAFGLVASLTALDLGMQIHGRLLRSGIHHDGFLRNSLVDFYSKCGEMEKASFVYQGFPKCNASDRCSKNNNKSLPHSFSQSSMVTGYVQNGKLQEALKMFTTMVTDRFEVDKFTLTSIVTGCANAGLLELGQLIHAQIVKAGQPFDIFLSSSMIDMYAKCGELDDAWSIFRETKVRNTVLWTSVISGYGLHGRGRDAIQMFNWMLAEGIKPNEISFVGLLTACSHAGLLNEGIHYFMVMKEEFGIKPQVEHFTCMVDLFGRSGRLDDIKDFISKNKISNVASVWRSFLSSCRIHRNIEMAKWVSDKLFELEPPTAGSYILLSNTCASGDKWDDVAQVRGLMKDMGLMKHPGQSWIQLKNQVHSFVMGDRSHPEEAAIYSYLNELIQRLKEIGYLTEANIVMQDVEEEQKELLLAFHSEKIAIAYALMKTSTGSPIRIMKNLRVCTDCHSFMKCTSLLLEREIVVRDLRRFHHFISSTVTVLVEIIGKVRMIYNACELFHKVSQYLETCGFYCHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WTR6</accession>
    <name>A0A161WTR6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WTR6"/>
    <dbReference type="Gramene" id="KZM91987">
        <property type="protein sequence ID" value="KZM91987"/>
        <property type="gene ID" value="DCAR_020648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043758296" evidence="2">
        <location>
            <begin position="27"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="80" mass="8781" checksum="0BFE99C6D181F24D" modified="2016-07-06" version="1">MEKKAFATIILTVLLFHSCFLLSATASDRLLFKEESGGAANEMSNIELTEMKDYPPPGANCKHDPENCPPSSSSTDNFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X4Z7</accession>
    <name>A0A161X4Z7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0728301</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
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        <reaction evidence="13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with RAP74.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="WOH08850.1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RNA polymerase II C-terminal domain phosphatase-like 3"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CPDc"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
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            <end position="64"/>
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            <end position="155"/>
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            <begin position="1"/>
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            <begin position="133"/>
            <end position="142"/>
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            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00001936"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X729</accession>
    <name>A0A161X729_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0729059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH09601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_025308"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF73">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH09601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="54" mass="6138" checksum="3CDD668C68D23CE2" modified="2016-07-06" version="1">MGTATFVEIILAIILPPLGVFLRFECQVEFWICLLLTFLGYLPGILYALYVLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XXL9</accession>
    <name>A0A161XXL9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_010141</name>
        <name evidence="12" type="ORF">DCAR_0311449</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XXL9"/>
    <dbReference type="STRING" id="79200.A0A161XXL9"/>
    <dbReference type="Gramene" id="KZN01387">
        <property type="protein sequence ID" value="KZN01387"/>
        <property type="gene ID" value="DCAR_010141"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210382"/>
    <dbReference type="OMA" id="ARMNCKE"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04133">
        <property type="entry name" value="Rop_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000336">
        <property type="entry name" value="rac-like GTP-binding protein RHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN01387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG92189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="197" mass="21504" checksum="4640D0FCC3BF8FB1" modified="2016-07-06" version="1">MSASRFIKCVTVGDGAVGKTCLLISYTSNTFPTDYVPTVFDNFSANVVVNGATVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIVLVGTKLDLRDDKQFFVDHPGAVPITQAQGEELRKTIAAPSYIECSSKTQENVKGVFDAAIKVVLQPPKTKKKKGKAQKACSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YGD0</accession>
    <name>A0A161YGD0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625812</name>
        <name evidence="3" type="ORF">DCAR_0625877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YGD0"/>
    <dbReference type="Gramene" id="KZM92089">
        <property type="protein sequence ID" value="KZM92089"/>
        <property type="gene ID" value="DCAR_020546"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227475"/>
    <dbReference type="OMA" id="GPYWPAE"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45180">
        <property type="entry name" value="OS01G0307686 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45180:SF1">
        <property type="entry name" value="OS01G0307686 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="39"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="265" mass="30053" checksum="6D680955823BD7DD" modified="2016-07-06" version="1">MAELFIKQAKQYLATRPNYPQQLFQFIASKTPSHDLVWDVGTGSGQAATSLAEIYKKVIGTDTSPKQIELAPKLPNVEYKCTPADLSVAYLDSHIAAKSTVDLVTVAQALHWFDNLGFYQQVKYVLKKPHGVIAAWCYTVPQVNEAVDAVFKPYYAVDVEPYWDPARKKVDNEYRSIEFPFEPVDGLDDTGPFQFKTESLMSLDEYFMYLRSWSSYQTAKDKGVELLNDRVLEEFTRAWNQDGTESKIVVFPVHLRIGKVGSLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZT63</accession>
    <name>A0A161ZT63_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CSC1-like protein HYP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_021663</name>
        <name evidence="16" type="ORF">DCAR_0624552</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH05139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_021663"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227586"/>
    <dbReference type="OMA" id="FSVTFIR"/>
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        <property type="entry name" value="CSC1/OSCA1-like_N"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="13">
        <location>
            <begin position="5"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="14">
        <location>
            <begin position="186"/>
            <end position="338"/>
        </location>
    </feature>
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        <location>
            <begin position="351"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="230"/>
            <end position="290"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM90972.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH05139.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="709" mass="80402" checksum="650CEF440042B449" modified="2016-07-06" version="1">MILSALLTTVGINIGLCVLFFTLYSVLRKQPGNASVYAPRLVAEGKMLRSDFNLERLLPSASWVKKAWQTSEEELLSLSGLDGVVFMRIFEFSIKVFSFAMIFGIILLPINYVGNQLTMDYMDLPNKSLESFSISNVNDGSNRLWIHFSVAYIFTGFVCYLLYSEFKYISSKRIACFNSSEPKVHHFSVLVRGIPVSSGISFSESVENFFTLYYPSTYISHVIVRRTSKLQKLISDADDLSKRLLQMKSASYTQQRVGRASFLGRKVDLVDHYEKKLEDLVENVRMEQTLITGQEVPAAFVSFKSRFGAAIVLHVKQGINPTEWNTEAAPEPQDVYWPFFSASFTQRWILKYVAIAACIVLVVLFFGPVVLVQSLTHLDQLETWFPFLKGILRVTFVSQVITGYLPSLILQLFQHYVPPAMIMLSSIQGYIARSHIEKSACYKILFFTIWNVFFVNVLSGSVLYQANVFLELKNIPSVLAVAVPGQAEFFIAYVVTTGWTNTSSELFQMFSLIWSFIKRKILGRADDKLVVPSANYFSKIPKILLFGLFGITYLFLAPLILPFLLIHFCLGYIVYRNQLLTVYSPKYDSNGKFWPIVHNSTIFSLVLMHIIAIGIFGLKKMPLASGFMIPLPVMTLLFNNYCQRRFIPLFKSYPAECLIKKDRDDQSSGTMDAFYERLATAYRDPALAPIHTLGISDGRNAPLLQATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZUA1</accession>
    <name>A0A161ZUA1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_021123</name>
        <name evidence="4" type="ORF">DCAR_0625211</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZUA1"/>
    <dbReference type="Gramene" id="KZM91512">
        <property type="protein sequence ID" value="KZM91512"/>
        <property type="gene ID" value="DCAR_021123"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226509"/>
    <dbReference type="OMA" id="WISHWSS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007830639" evidence="2">
        <location>
            <begin position="26"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
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            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="404"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM91512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH05790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="418" mass="38195" checksum="BB0C3E9CAD2DCDEC" modified="2016-07-06" version="1">MKFKSINFLTLLFVAIIFIAVIAKAEEEKSVEASEMHNTNILGRRGGYVNFDDKWGQGHGHHHGCGKKNCGQSGGYGGQHGGAQPGGGGDQSGGDSGQQGGAQPGGGGDQSGGGSDQHGGAQPGGGDQQGGDVGQSGGGGGQHGGYGGQHGGAQPGGGGDQFGGGSGQQGGAQQGGGGDQSGGATDQHGGAQPGDGGQQGGYGGQGGAQPGGGGGQQGGGSGQQGGDAGQSSGGGGQQGGYGGQGGAQPGGCGGKPGGGCDQSGGGNGQQGGAQTGSGGQQGGDVGQSGGSGGQHGDQSGGAQPGGDVGQHGGSGDQQGGQPGEGAQSGGDVGQSGGSGGQQGGYGGQPSEGCGQHGGHPNGSNCALPGGDVGQQGGSGGQQHTGGDQSSGNVGKPHDDTSHQGGYGGQPKSGGKGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZUR6</accession>
    <name>A0A161ZUR6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_020923</name>
        <name evidence="16" type="ORF">DCAR_0625415</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZUR6"/>
    <dbReference type="Gramene" id="KZM91712">
        <property type="protein sequence ID" value="KZM91712"/>
        <property type="gene ID" value="DCAR_020923"/>
    </dbReference>
    <dbReference type="OMA" id="GVFSSHQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007830652" description="Subtilisin-like protease" evidence="11">
        <location>
            <begin position="26"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="31"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="138"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="645"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZM91712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH05992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="82513" checksum="025445E7E64BD6C6" modified="2016-07-06" version="1">MAAQFQFSVCLLLMTISQFISASLAHEMNCYIVHMDISAMPKAYSNHHSWYLATLASITDNSKLTTTSVPSTKIIYTYTNVMHGFSAILSDSELESLSSLPGYVSSFRDAPVNLDTTYSFKFLGLNSNYGAWPDSDYGRDVIIGVIDTGIWPESKSYDDYGMTPTPLKWKGACEIGTQFNSSMCNKKLVGARYFSKGLLANNPNLTISMNSPRDTDGHGTHTSSTAAGNYVDNASYFGYAYGTARGMAPHARVAMYKALFNEGSVSSDILAAIDQAIEDGVDVLSLSFGLNDVALFEDPVAIATFAAIEKGIFVSTSAGNQGPLLETLHNGTPWVLTVAAGSIDREYVGRIILGNGVTITGQTLYPNNLTHSQNFPLVFMGECDDVKKIGTGFVVCQDKTGALSEQVYACLNANVSGGIFITNNSDLGFFRDSSFPALFLNHQDGETILKYINMNQKAQASFEFQKTSIGAKAAPKVAAYSSRGPSASCPFVLKPDIMGPGDLILASWPSNVAATEGSSQYFSNFNLLSGTSMSCPHVAGIAALLRGVHPKWSPAAIRSAMMTTSDFLDNKYNPILDSGALNHPASPLASGAGHVNPNKALDPGLIYDVKAADYINLLCALNYTSKQIQTVTRSASYSCSTASLDLNYPSFIAYFNANDTSNSNELTVQEFHRTVTNVGDKTSVYEAKITHMDGLNVNVVPEKLSFKDKYEMQSYRLSIQGPRRMKNKIIHGSISWIEVSGRHVVRSPIVATNLSSRILSGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZXQ8</accession>
    <name>A0A161ZXQ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7,8-dihydroneopterin aldolase</fullName>
            <ecNumber evidence="8">4.1.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_020685</name>
        <name evidence="11" type="ORF">DCAR_0625670</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 7,8-dihydroneopterin into 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin tetrahydrofolate. Can use L-threo-dihydroneopterin and D-erythro-dihydroneopterin as substrates for the formation of 6-hydroxymethyldihydropterin, but it can also catalyze the epimerization of carbon 2' of dihydroneopterin and dihydromonapterin.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homooctamer. Forms a hollow cylinder assembled from two ring-shaped tetramers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DHNA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="8"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZXQ8"/>
    <dbReference type="STRING" id="79200.A0A161ZXQ8"/>
    <dbReference type="Gramene" id="KZM91950">
        <property type="protein sequence ID" value="KZM91950"/>
        <property type="gene ID" value="DCAR_020685"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224701"/>
    <dbReference type="OMA" id="INPPIGG"/>
    <dbReference type="OrthoDB" id="1863886at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000003">
        <property type="entry name" value="7,8-dihydroneopterin aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00525">
        <property type="entry name" value="folB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase/epimerase" evidence="9">
        <location>
            <begin position="12"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM91950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH06247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="14852" checksum="53C8D608BC9B5440" modified="2016-07-06" version="1">MANKGEPKGDKLVLRGLKFHGYHGVKEEEKKLGQKFVIDVDAWMDLRPAGISDCLSDTISYTDIYRIVKEVVEGTPQNLLETVAELIASATLTKHPRISAVRIQVQKPHVSVPGHIDFLGVEITRYRSVEGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ABS1</accession>
    <name>A0A162ABS1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">WPP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_013756</name>
        <name evidence="4" type="ORF">DCAR_0417245</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ABS1"/>
    <dbReference type="STRING" id="79200.A0A162ABS1"/>
    <dbReference type="Gramene" id="KZM98882">
        <property type="protein sequence ID" value="KZM98882"/>
        <property type="gene ID" value="DCAR_013756"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219341"/>
    <dbReference type="OMA" id="DKHWLRS"/>
    <dbReference type="OrthoDB" id="680851at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044696">
        <property type="entry name" value="WIP1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34562:SF8">
        <property type="entry name" value="WPP DOMAIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34562">
        <property type="entry name" value="WPP DOMAIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM98882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97904.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="62784" checksum="6C3EEEF1DB628E26" modified="2016-07-06" version="1">MDLESESSVIESVEDNNELIVRSGSKLVDGSKDCVLENVGKSSADEVRPEGGQSKPAGSIGSSPKVKKGYGLKKWKRIPRRESSKERGNSVDNDRGKRVLPNATNDSRNRAGSADTKQRSEGSVSSTNAVVKSPGLASGGFGYGLGFGPINAVATDSENSEDRSSRSSTAASAPRWKSEIAALGGYLGDNNGVISSNRDHNVAAQMGQQGKSRTDSGKKPRGNQVKIEKENSHSSMESDSRSSNFVFLQGASSVTSKGRQSRRSANYDGENSDDEAQGSEQRFAEELQAGFRKNMAECEDVSQSYLPADLNWKRKGVNSENIGLSADQDPLLESITSLQSAHEALAQELLKFREIGKDDSDEVQDSIPNLTEQQQYGETRQTRRSSLDNERVNLKWNVNKLESKLADSSSVLEVKESKVIELEDNTARLEENRKISELEHKNSRDQNLELESLFKQKIEAEVGFLVLYSSLSSGVNKADEIKFLEEQKTLASEQAKIVNFSENIGTRARMLKRQVDELEMKKEIGEYVKLEKRMCKFASLFFIQLVLLFAALCFFVLQLVPEYTEDVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="2">DUF6821 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">DCAR_014068</name>
        <name evidence="4" type="ORF">DCAR_0416881</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A162ABW2"/>
    <dbReference type="Gramene" id="KZM98570">
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        <property type="gene ID" value="DCAR_014068"/>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045883">
        <property type="entry name" value="At4g13530-like"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20705">
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    </dbReference>
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        <location>
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            <end position="298"/>
        </location>
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    <feature type="domain" description="DUF6821" evidence="2">
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            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM98570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="359" mass="39651" checksum="EBE3F444755669FA" modified="2016-07-06" version="1">MDLDFVGLEDWELLPSSDSNPVEDIKGFQVIDNDSGVLIRPDYFDSRSEYLKSNQESSGAQSLKRVGDEESGIGVGFDGVEEIDLGGAENERIEVGFVEKSELEVGFDWVGETVSRDLGGVESEKYDLGLMKKSELEVGFDGVGETAVRELGFAENEKNELQQGEMGEEVVGRDLGVDESEKTEVGLVGGVDESENSGILLSDFIGVEGNSEMSDGADSNFRDELVLGGGGQGDKHCDDVDIVRGERESGGEVEKRYMVWWKLPFEFLKYFVLRTSPVWTVSMAAAVMGFVILGRRYYSMKRKSRSVQVKVTMDDKKISQFKSRVVRLNEAFSVVKHVPIIRHSLPAAGVTPWPAMTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UMV1</accession>
    <name>A0A164UMV1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_026179</name>
        <name evidence="15" type="ORF">DCAR_0730053</name>
    </gene>
    <organism evidence="14">
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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        </lineage>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UMV1"/>
    <dbReference type="STRING" id="79200.A0A164UMV1"/>
    <dbReference type="Gramene" id="KZM89104">
        <property type="protein sequence ID" value="KZM89104"/>
        <property type="gene ID" value="DCAR_026179"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195036"/>
    <dbReference type="OMA" id="FIVYRAQ"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000468">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056562">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056563">
        <property type="entry name" value="LysM3_LYK4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF9">
        <property type="entry name" value="FAMILY PROTEIN _ PEPTIDOGLYCAN-BINDING LYSM DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23472">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23473">
        <property type="entry name" value="LysM3_LYK4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853704" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="13">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="13">
        <location>
            <begin position="187"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="321"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZM89104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH10584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="69433" checksum="B507AB0AC2DE3458" modified="2016-07-06" version="1">MIHFSNSIGLTIVLLLMMARNTISYQFYDKTNCTSDSEVFGSNYLCTSDKLPCQTFIIYRVQQGNETLNSISDLFNLENVSDILFYNNVSDSIQASLPPGHEIIIPITCECPDRFSRAVFSYNASASESLSSIACSVFGGLVKAQSLREENPYYGRREGPEYSLVHVPVRCACPDSLDLSEGIKYLVTYPIMGSDSTDLIARKFGVTQQIIWDANRLSTYTAIYPRTVLIIPTTSVPSLNYVVASPGENPSPPQKSSPLGDIIPGEDRKKNVKFAAYVAVAVAGAVALVGVSFCMVILGIKRRSHLRFKNLSPIVPRSSNFSPDFLDGMSKLNNSLICFNLDELRSATEDFSEGSLIGSSLYRGKIGDTCLAIEEMRSMKEANHVVYILTKTNHLNVVKLLGFCYGTRPFLLFEYAENHSLRYCLSNSKLAEQLTWAKRMQIAFDLAVGIHYIHYCTKPTYVHRNINSKNVLITADWRAKISDFKLAKPLVFSDQTGETDWNESVIVGKKGYLSPEYMNEGHASTKVDVYAFGVVLLQLLSAKEAISEEKFLKDSIEFLTEETEDSAACLEKLKEFMDPVLNGDYNVGDAMNLALLAKGCVEKDTLHRPNMNDVLKAISRMLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UUE6</accession>
    <name>A0A164UUE6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0622671</name>
        <name evidence="18" type="ORF">DCAR_0622749</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="6"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UUE6"/>
    <dbReference type="Gramene" id="KZM89375">
        <property type="protein sequence ID" value="KZM89375"/>
        <property type="gene ID" value="DCAR_023262"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192984"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000027">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054694">
        <property type="entry name" value="Parkin-like_IBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22605">
        <property type="entry name" value="IBR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="117"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH03275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH03352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="518" mass="58945" checksum="B4E47D9DAD5CD98D" modified="2016-07-06" version="1">MDTEDDTFSDDHDVSDDVVSSDSDFTPETNSGDHREKSYKVLSEEDIKRIQDDRIERVTTSLYVSSGVARVLLNKFKWNVNELQESWFTDEGKTRKDVGLAESRKKHVGTKKIEKLDQLNCSICFEDFVCDVRVDNCVLCDHQYCNCCWNGYISNAISEGSGCLSLRCPDPDCDVVVTDELVSVIVGGDDKLRYERLCVRAYVEGNEKVKWCPGPDCGFAVEYDDVVGSGEGYDVVCGCGYCFCCNCGEDGHRPVDCETVAKWVEKNNAESENTTWILVYTKPCPKCRRPIEKNDGCMHMTCRKPCGYSFCWVCLDDWGKHAYNSCNSYAGRVVKENNDEERVKQRARISLERYTHYFERWAANNKSMKKSFADLQRMRDENHDVLLAKHAQSSSQLKFVLDAWTQIVECRRVLKWTYAYGYYLPEDNIAKTKLFEYLQGEAEAALENLHHCAEKELTEFLRDGTTAEFNTTFREKLANLTSVTKTYFANLVEALENGLADVDSGSANKKLKTGSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VXL8</accession>
    <name>A0A164VXL8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0624597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VXL8"/>
    <dbReference type="Gramene" id="KZM91005">
        <property type="protein sequence ID" value="KZM91005"/>
        <property type="gene ID" value="DCAR_021630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000154">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH05183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="29943" checksum="A91B46302E709122" modified="2016-07-06" version="1">MINFIRRKVLGGRKPQEKLYDDDEEKKAYEGSDVLDPIRCGTSFNTAKETIFFWRDFLKVLVHPDFHFPDKPKGFRYSVHHLALFLAWELLRGISVSLGLEASYIHDVLNLESSLQVFVANLYPPCPQPELALGMPPHSDHGLLNILIENGVGGLQLLHNGKWVNVRAPPNSFLVNTCDHLEILSNGRYKSVVHRAVVNNATTRLSLAIANGPSLDTIVRPAPGLTDGANNPPAYTPMKYKEYLQLQQGNKLDQKSVLDRIRLQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W077</accession>
    <name>A0A164W077_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_021537</name>
        <name evidence="3" type="ORF">DCAR_0624711</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W077"/>
    <dbReference type="STRING" id="79200.A0A164W077"/>
    <dbReference type="Gramene" id="KZM91098">
        <property type="protein sequence ID" value="KZM91098"/>
        <property type="gene ID" value="DCAR_021537"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227239"/>
    <dbReference type="OMA" id="FMTGGHL"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820:SF21">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE 3B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM91098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="263" mass="27949" checksum="CB179817D4E551DE" modified="2016-07-06" version="1">MATPPASLPLNKLQDKVAIITGGASGIGEATAHLFAEHGARAIVIADIQDELGQTVANSIGPHKCKYFHCDVSDEAQVQALVDFTAHTFGPLDIMFSNAGIVSHSDQDVLSLDMPQLDRVFAVNVRGMAACVKHAARAMVASRVKGKIVCTASIAGKTGGKKRTDYFMSKHAVVALVKSASKQLGEFGIRVNCVSPYAIATPLMTKLYNKGKEEVEKIYQPFTSLKEEALKVADIANAVLFLVSDDSAFVTGHDLIVDGGFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z3A8</accession>
    <name>A0A164Z3A8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_018500</name>
        <name evidence="7" type="ORF">DCAR_0521173</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z3A8"/>
    <dbReference type="STRING" id="79200.A0A164Z3A8"/>
    <dbReference type="Gramene" id="KZM95258">
        <property type="protein sequence ID" value="KZM95258"/>
        <property type="gene ID" value="DCAR_018500"/>
    </dbReference>
    <dbReference type="OMA" id="IAHEQHE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21504">
        <property type="entry name" value="PPP2R3A_B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000003">
        <property type="entry name" value="Phosphoprotein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.230:FF:000002">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit B''alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000067">
        <property type="entry name" value="serine/threonine protein phosphatase 2A regulatory subunit B''beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
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    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF-hand_13"/>
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    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF23">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B''ALPHA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
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        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
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        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM95258.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH01788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="537" mass="62044" checksum="02A0DD132CEBBC2C" modified="2016-07-06" version="1">MEMDIARDVTDLDPELLQLPEVSPLALKTSPQIAEELFGQWLSLPDTGRLVKSLIDDAKTGTSFGASGNSTSTNASGISPLPSLFPAANTPPLSPRSAVGSPRTMKQRASFSSLGSPLKLVKENVREVIPQFYFQDGPPPSEELRVQCNSRVDEIFNRNMDGLQVNEFKTVTKDICKLPSFLSTTLFLKIDTNCTGLVTRDAFIKYWVESKMITMDVATQIYKILKQPDCKYLTQADFRPALKELLATHPGLEFLQNTPEFQERYAETVIYRIFYYINRSGNGHLTLRELRRGNLVAAMQHADEEEDINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIKYGNHALTYRIVDRIFSQVPRKFTSSIEGKMGYEDFVYFMLAEEDKSAEPSLEYWFKCIDLDGNGVLTRNEMQFFYEEQLHRMECMGQEPVLFEDILCQIVDMISPEREDYITIQDFRGCKLSGNVFNILFNLNKFMAFESRDPFLIRQEREDPTLTEWDRFAHREYIRLSMEEDGESNGSAEVWDESLEAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XFU2</accession>
    <name>A0A165XFU2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0416416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays a role in anchoring the complex to other cellular components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">EF-1 is composed of four subunits: alpha, beta, delta, and gamma.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_014507"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="249703at2759"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor 1-gamma 1"/>
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    </dbReference>
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        <property type="entry name" value="Elongation factor 1B gamma"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF1B_G_C_sf"/>
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        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA 3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GST_N"/>
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    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG97077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="418" mass="47427" checksum="0D6F2825BF3AB79A" modified="2016-07-06" version="1">MALILHAGNGNNNAWKALIAAEYSGVKVELAKDFQMGVSNKTPEFLKMNPMGKIPVLETPDGAVFESNAIARYVARLNPDTALFGSSPIDYARVEQWIDFASLELYTNLSRWGYPRMGYGVYLPPVEEAAIAGSKRALGALNTHLASNTYLVGHSVTLADIIITCTLAFAFSRLLTKSFTSEFPHVERYFWTMVNQPNFSKILGEIKQTDSVPPVPSAKKETAKPKAKDEPKKEVKKEAVKPKTEEVVEEDEAPKPKAKNPLDLLPPSKMILDDWKRLYSNTKTNFREVAVKGFWDMYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLARKYAFGKMLVIGSEPPYKVQGLWLFRGKEIPKFVMEECYDMELYNWTEVDITDEAQKERASQMIEDHEPFYGEALLDAKCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZVL8</accession>
    <name>A0A165ZVL8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0310449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZVL8"/>
    <dbReference type="Gramene" id="KZN00486">
        <property type="protein sequence ID" value="KZN00486"/>
        <property type="gene ID" value="DCAR_009240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG91201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </evidence>
    <sequence length="840" mass="92252" checksum="42B6DB6FDEBEA03A" modified="2016-07-06" version="1">MAMDVAHGESNEGSITKHLDNGKYVRYTAEQVEALERVYMECPKPSSMRRQQLIRECPILANIEPKQIKVWFQNRRCREKQRKESSRLQTVNRKLSAMNKLLMEENDRLQKQVSQLVCENGYMRQQLHTVPSVTTDLSCESAVTPQHSLRDANNPAGLLSIAEETLTEFLSKATGTAVDWVQMPGMKPGPDSVGIFAISHTCSGVAARACGLVSLEPTKIVEILKDRPAWFRDCRRHEVFTMLPTGNGGTIELLYTQIYAPTTLAPARDLWTLRYTTSLDNGSIVVCERSLSGSGAGPQAAASAQFVRGEMLPSGYLVRPCDGGGSIIHIVDHLNLQAWTVPEVLRPLYESSKVVAQRMTITALRYIRQIAQETSGEVIYGMGRQPAVLRTFSQRLSRGFNEAINGFNDDGWSVINCEGSEDVIVAVNSAKNLVSTSDLSSSLSLVGGVLCAKASMLLQNVPPAVLVRFLREHRSEWADFNIDAYSAASLKGSSYAYPEMRPTRFTGNQIIMPLGQTIEHEEMLEVIRLEGHPLGQEDSFMSRDIHLLQLCCGVDENAVGACSELVFAPIDEMFPDDAPLLPSGFRIIPLESKSGDVQDSITTNRTLDLASSLEVGPAANHAASDSSACYNMRSVLTIAFQFPYENNQPENVATMARQYVRSVVSSVQRVAMAISPSGMSLTAGPTVSPGAPEAQTLANWICQSHRYHLGTDLLSSDSVGGDTSLKNLWHHPDAILCCSLKSLPVFIFANQAGLDMLETTLVALQDITLDKIFDDTGRKAFNSEFAKIMQQGCAYLPGGICMSTMGRHVSFEQAIAWKVLAAEENTVHCLAFSFVNWSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DLZ1</accession>
    <name>A0A166DLZ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_006249</name>
        <name evidence="6" type="ORF">DCAR_0207050</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DLZ1"/>
    <dbReference type="STRING" id="79200.A0A166DLZ1"/>
    <dbReference type="Gramene" id="KZN05412">
        <property type="protein sequence ID" value="KZN05412"/>
        <property type="gene ID" value="DCAR_006249"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207967"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF154">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="4">
        <location>
            <begin position="285"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN05412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG87818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="709" mass="80877" checksum="B2BB9ECC36B58FB8" modified="2016-07-06" version="1">MTRAVELDRLIAARDVLRNGIAESRDMAWAISEAGCRLEEMNQRLPALEGSLKNIARKCEMNKFHVDHAVGPAAAVLSIYNVVIQLQEWLVVADLRDSDVLIPYVYQVKRLEEALGFLTQNCKLAVLWLEDVVELVENNAVRAQYNHCFFSGHVRKSLGILRNLQAMEERSRLDGGLLHAAFTNLQKEFRHLLVQNTYPLKQQTNDTDSTAVSLLAYPVPVVEKLQLILERLTANNQLDRCVSIYTEVRAFNLQATVQALDFGYLETTSLSEFDSLQSVESCIEKWSDDLEFVVKHLLELEYRASNAVFNREVDGLDVANECFAKTVMKCGLLQRFIKFANTITRGKKEAVKLLKLLDIFESLNKLRMNFNRLFGGQTCVEIQSQMRDLIKKVINEACDIFWELPAHVESQRQSTPPADGGIPRLLSFVVDYSNELLSEYYRPILTQVLEIHWSWNCNHAPKEDLEKQRQQFLLNEELHYKKIMKALELNIQAWGRTYKDSTLSYIFLMNSHWYLCNNLKGTEIGDVMGDLWLRGHEECVEHYSSVFVRESWGRLAPLLTEEDEGLQVLLSGGGTAVLRDSMKRRIKAFTEAFDEMYRKQCHWVVPDTGLRWKICQLLVRAVVPVYSNFILSCISNLEAEAAEKDGNFSPTLAGKRCVNNSKESLENMLCSLFQPKLAKKHGASAKSRHLIGKIKNVVTNHFLSTSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F8X4</accession>
    <name>A0A166F8X4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_008323</name>
        <name evidence="2" type="ORF">DCAR_0209408</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F8X4"/>
    <dbReference type="Gramene" id="KZN07486">
        <property type="protein sequence ID" value="KZN07486"/>
        <property type="gene ID" value="DCAR_008323"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN07486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="95" mass="10713" checksum="965EE9D139523371" modified="2016-07-06" version="1">MRDFRKNLDDMKSAYERCLNNFEVVLALYPGNAELAKLRDENRKFFQLFEETNPLSKMMLGGTRKVRGNAVEEQGDDCSFAPSFSLGLCLDGAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FD83</accession>
    <name>A0A166FD83_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_008481</name>
        <name evidence="5" type="ORF">DCAR_0209575</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FD83"/>
    <dbReference type="STRING" id="79200.A0A166FD83"/>
    <dbReference type="Gramene" id="KZN07644">
        <property type="protein sequence ID" value="KZN07644"/>
        <property type="gene ID" value="DCAR_008481"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206889"/>
    <dbReference type="OMA" id="MCANLLG"/>
    <dbReference type="OrthoDB" id="696558at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.110.10:FF:000003">
        <property type="entry name" value="pEARLI1-like lipid transfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007873229" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="55"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN07644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG90332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="138" mass="14135" checksum="E1ECBF2DA1700675" modified="2016-07-06" version="1">MASKTLASTAFVLALNLLLFSLVSSTYCPPPPKPKCPPPPAPKPKCPPSVPEATCPRDTLKLGACANVLGGLVGLVVGAAPKKPCCSLLEGLVDLQAAVCLCTAIKANVLGINLNVPVDLSLILNYCGKKVPTGFQCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IBA5</accession>
    <name>A0A166IBA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0103825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IBA5"/>
    <dbReference type="Gramene" id="KZN10954">
        <property type="protein sequence ID" value="KZN10954"/>
        <property type="gene ID" value="DCAR_003610"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198789"/>
    <dbReference type="OMA" id="RRNTWHY"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000808">
        <property type="entry name" value="Iridoid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF0">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="3">
        <location>
            <begin position="33"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG84641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="387" mass="43951" checksum="F6B27A1A367430BA" modified="2016-07-06" version="1">MSLWWSGAVAAAKKRMGQLEYASAPSRYNSVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPPWKVDERVDYIQCDICDLDETRLKLSMLQDITHVFYVTWANRPNESENCEINGKMLRNVLNALIPNSPNLKHICLQTGRKHYVAPGSEDHDPPYTEDLPRLDSPNFYYILEDILFEQVGKKEGLTWSVHRPAPIFGFSPYSLMNIIGTLCVYATICKHEGVPMRFPGTEEAWNCYSDASDADLIAEHQIWAAVDPYAKNEAFNCSNGDVFRWKHFWKVLGQQFEVECGYLEAGERLSLAEMMKDKGPVWDKIVKEKGLVPTKLEEVAAWWFADGMFSGPCPLISMNKSKEHGFFGFRNSKSCFIYWIDKMRSYRVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IUC2</accession>
    <name>A0A166IUC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Equilibrative nucleoside transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_004155</name>
        <name evidence="9" type="ORF">DCAR_0104435</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IUC2"/>
    <dbReference type="Gramene" id="KZN11499">
        <property type="protein sequence ID" value="KZN11499"/>
        <property type="gene ID" value="DCAR_004155"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204807"/>
    <dbReference type="OMA" id="HITIDCI"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF38">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOTIDE TRANSPORTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN11499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG85247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="46025" checksum="0625F03B0DA1F07F" modified="2016-07-06" version="1">MPASSETPVKLEGTPLAILVCWFLGLGSLVAWNSMLMIGDYYYALFPDYHPSRVLTLVYQPFALGTMAILAYNESKIDTRKRNIAGYILFFLSTLALLVLDLATSGKGGIGNYIGICAFVGAFGVADAHVQGGMAGDLSFMRPEFMQSFFGGLAASGALTSGLRLMTKAAFENSGDGLRKGTMLFLAIATFLEFLCIFLYALVFPKLPVVKYYRSKAASEGSTTVSSDLAAAGIQKQEIQQAEDDADSKKRLTIKELCYQNIDYLLDMFLIYVLTLSIFPGFLYENTGEHRLGSWYVLVLMAMYNVFDLIGRYVPLLEFIKLESRKGLTIATLSRFLLIPAFYFTAKYGDQGWMIFLVSFLGLSNGYLTVCVMTVAPKGYKGPEQNALGNLLVLFLLGGLFAGVSLDWLWLINSGKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IVM6</accession>
    <name>A0A166IVM6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_004197</name>
        <name evidence="10" type="ORF">DCAR_0104491</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG85303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166IVM6"/>
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        <property type="protein sequence ID" value="KZN11541"/>
        <property type="gene ID" value="DCAR_004197"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214824"/>
    <dbReference type="OMA" id="KITQGHS"/>
    <dbReference type="OrthoDB" id="147332at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN11541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG85303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="98" mass="10961" checksum="35AD0611BC84E626" modified="2016-07-06" version="1">MVCDKCEKKLAKVIVPDKWKEGASNTTEGGGRKINENKLLSKKKRWTPYGSGTTKCIICKQQVHQDGKYCHTCAYTKGVCAMCGKQVLDTELYKQSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J5M7</accession>
    <name>A0A166J5M7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J5M7"/>
    <dbReference type="Gramene" id="KZN11820">
        <property type="protein sequence ID" value="KZN11820"/>
        <property type="gene ID" value="DCAR_004476"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224852"/>
    <dbReference type="OMA" id="CLEMDKV"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045182">
        <property type="entry name" value="JINGUBANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844">
        <property type="entry name" value="F-BOX AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844:SF213">
        <property type="entry name" value="OS01G0232200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="50867" checksum="537945FD75A5144F" modified="2016-07-06" version="1">MEHKIKRSLLNYFHEEGKSMHPQIHLPQQQQISRNLDSLLRSPSTSVVSPSQNIMHSMMPPPSPDSPWTLSPLQSPSPPLLCHCIASLHRLDGTVHAIAAINGTVFTGSDSSRIRAWRQPDCFERGYLKASCGSVRAMLAYGNVLFTSHKDFKIRTWIVTVSDNFKAKKISTLPRRNSLFTFSRRTSEGHKDYISCMAYYNAEGLLYTGSWDKTVKVWRVWDNLCVDTFLAHDDNVNAVVVNQDDGVLFTCSCDGSIKIWRRIYGQSSHTLTMTLKAHSSPINAMALSTSVIDSYVLYSGSSDGFINFWEKEKVSGRYNHGGVLQGHRFSVLCLVALGKLIFSGSEDTTIRVWRREEGKNSHECLAVVDAHRGPVKCLSASLEVDKDVGVLLYSAGLDQNFKVWRIKVMLEERNTGHCFETASNNKLREFEMSPVLSPSWVERKIHGSHPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175Y9U2</accession>
    <name>A0A175Y9U2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175Y9U2"/>
    <dbReference type="Gramene" id="KZM80309">
        <property type="protein sequence ID" value="KZM80309"/>
        <property type="gene ID" value="DCAR_031895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043702591" evidence="2">
        <location>
            <begin position="24"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="16119" checksum="51934E537F1E7909" modified="2016-09-07" version="1">MSSKSSLPLVLFLFLNLLFTGLAVSSSDCDSPPKHSSKHKHKHHDRNSNSPPMSNSNPPKLPPRFPLPLSPRSAPGTPELPPVLGGLLGVRAGTAPKKPCCRLFGGLVEVESAVCLCTAIKANVLGSNLNIPISLGLLLNVCDIQTPPGFQCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDX8</accession>
    <name>A0A175YDX8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0933924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDX8"/>
    <dbReference type="Gramene" id="KZM81839">
        <property type="protein sequence ID" value="KZM81839"/>
        <property type="gene ID" value="DCAR_029452"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="31"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="265" mass="30508" checksum="EE3452665572E7C2" modified="2016-09-07" version="1">MLSIWYDMIKPQLNYWQLAQKVKGDEDVGVKVCVRVQKSKLITRNAQRVAINLIFVDALGGRIHATIPAPYIGQLENYFTEGETYDVNNFVVRRYADMQHGRCFKNDIYIQLNHMTEVMVTGGVDYIQQHVFEFTDLDALYATAHEQKNLIDVVGILEQAGPLTHFRNRIGQEEPCVEFRITDMFTSARAFFYNEMAEEFHQAIQQANQHPIVVIISSCKPQMFSEEPNVTNFQATRFFINPNHEAVDDLRNALSVANAKQLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YJ73</accession>
    <name>A0A175YJ73_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0936177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YJ73"/>
    <dbReference type="Gramene" id="KZM83609">
        <property type="protein sequence ID" value="KZM83609"/>
        <property type="gene ID" value="DCAR_031178"/>
    </dbReference>
    <dbReference type="OMA" id="WLEINTC"/>
    <dbReference type="OrthoDB" id="1861598at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="PFYRE" evidence="3">
        <location>
            <begin position="353"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="447"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="264"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="525" mass="58975" checksum="F329E6AEB3822423" modified="2016-09-07" version="1">MAPGFYLQSKFCDDDEKNNHARLLNYKLSGTKRYSAPYGYKFEESLSDCFTPFVDETQNHKRLKETSCTDEFVSNEESIYSSSANTSGFYSGNSSGSTESLNSVSKPHFRDHIWAYTQRYLAAETMEEAAAALVAGDEDEVKEEGSADGMRLVQLLIACAEAVACRDKLHASTLLSELRANALVFGTSFQRVASCFVQGLSDRLALVQPLGTVGVVIPHLETMAISLEKEEALSLVYNICPHIQFGHFVANAMILEAFEGESSIHVVDLGMSLGLRHGHQWRSLMYSLANRAGKPRRLHITGVGNCAEHLLEIGDDLEQYAFNLGLNFEFSVVECNLENLQPRAFKLIEGETLVINSILHLHCVVKESRGALNAVLQKLHQLSPKYLILVEQDSSHNGPFFLGRFMEALHYYSAIFDSLDTMLPRYDTRRAKIEQFYFAEEIKNIVSCEGPARVERHERADQWRRRMSRAGFQSAPIKKITQTKQWLEKMKVSDGYTTVEEKGCLILGWKSKPIIAASCWKCSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPC9</accession>
    <name>A0A175YPC9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_027113</name>
        <name evidence="2" type="ORF">DCAR_0832455</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPC9"/>
    <dbReference type="Gramene" id="KZM85465">
        <property type="protein sequence ID" value="KZM85465"/>
        <property type="gene ID" value="DCAR_027113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM85465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="65" mass="7549" checksum="E038E9EE596FA1FC" modified="2016-09-07" version="1">MALCRSFTNTATPIAESFLRDVSRREDIKIAKASISYGCRNMACVKLRRALNQKNRHMQYLNQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YRB5</accession>
    <name>A0A175YRB5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Snakin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_026555</name>
        <name evidence="4" type="ORF">DCAR_0833105</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GASA family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM86023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YRB5"/>
    <dbReference type="STRING" id="79200.A0A175YRB5"/>
    <dbReference type="Gramene" id="KZM86023">
        <property type="protein sequence ID" value="KZM86023"/>
        <property type="gene ID" value="DCAR_026555"/>
    </dbReference>
    <dbReference type="OMA" id="HANRTKC"/>
    <dbReference type="OrthoDB" id="1850441at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003854">
        <property type="entry name" value="GASA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201">
        <property type="entry name" value="EXTENSIN, PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201:SF149">
        <property type="entry name" value="GIBBERELLIN STIMULATED TRANSCRIPT RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02704">
        <property type="entry name" value="GASA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044839" description="Snakin-2" evidence="2">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM86023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="10863" checksum="58848636D1D79DA4" modified="2016-09-07" version="1">MALRLFVFVALLLFCTLQVSSDSEIADEVKGPNSTPLTYADCQGLCQVRCARARKNVCMRACGACCARCNCVPPGTYGNREACGKCYTDMTTHGNRLKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W592</accession>
    <name>A0AAF0W592_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0102685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W592"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000416">
        <property type="entry name" value="p-loop nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056653">
        <property type="entry name" value="DUF7751"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF56">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24933">
        <property type="entry name" value="DUF7751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="768"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG83510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1025" mass="114225" checksum="FC92CCF1F147996E" modified="2024-05-29" version="1">MYARKLNSKYKKWGLISQQYKHVAGSDRSFGTCCKHTAILSNCSSRSVSTRRYISSSSLFRNVICGNTKLRVGSNANWSSSWLRYYSSEGDGRNANEDKCVPLKDVSDTGSDKIKTEKLDGDISLSDGHARLGEQDQKDWLHNEKITIENKRKEYPFLSRREKFKKEFSRRIVPWDKITVSWKTFPYFLHEHTKKLLVECTASHLKHKDCAAAYGSGLTSSSGRILLQSIPGTELYRERVVRALAQDLQVPLLVLDSSVLAPYDFGEECLSESESDEDNAESGAETTSDTEIEDENDASNEEDWTSSGEAKSDGDAEGFDARAAAVEAALNKLVPGHLEDFEKNVMGTSESSPESPEKEASKPSDEPKRQLKKGDRVKYVGPSRTEADNRIKLGKIPTSDGPTNAYTVIHGRPLSTGQRGVVCEMNGNVALVLLENSEKETGDASSDKISANHALHWIQAKDITYDLDAEAEDCYVAMEALSEVLHSVQPLIVYFPDFSIWLSRAVTKANRKAFAQKVQEIFDQVSGPVVLICGQNKGETGSKEKEKYTMILPNLGRLAKLPLSLKRLTEGLKPAKKSEDDEIYKLFTNVMVVHPPKEEDPLRTFNKQIEEDRRIVISRSNITELHKVLEENGLLCVDLLHVNADGVILTKSKAEKVVGWAKNHYLSSCLLPLIKGEKLYLPKESLEIALSRLKEQELISKKPSHTLKNLAKDEYESNFVSAVVPPDEVGVKFDDIGALEDVKTALNELVILPMRRPELFSRGNLLRPCKGILLFGPPGTGKTLLAKALATEAGANFISITGSTLTSKWFGDAEKLTKALFSFAGKLAPVIIFVDEVDSLLGARGGSFEHEATRRMRNEFMAAWDGLRTKDSQRILILGATNRPFDLDDAVIRRLPRRIYVDLPDAENRLKILKIFLDQEDLEPEFQMQQLANATEGYSGSDLKNLCRAAAYRPVQELLEKERKGVKDDVASILRPLNLDDFIQSKAKVGPSVAFDAASMNELRKWNEQYGEGGSRRKSPFGFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7J1</accession>
    <name>A0AAF0W7J1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0102277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7J1"/>
    <dbReference type="KEGG" id="dcr:108197389"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG83103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="378" mass="41708" checksum="74E2E3C476CED003" modified="2024-05-29" version="1">MLELLGSKRVRVGGDEEDSSAVNRHSRPKKLVPARRLGLKNRQQVRCSQQVVHSSSSGDEYEDNPETGERRKRVGFLFRTAPRPLLPNLLTKDDQWKLEGCSVQKRGRRAERAAGLVLGEFAGLLDSLATERSRCSRLQKRGRGLEQDLERAKARITDAVVAEQVTTLQATVDGSAGRLAELSKEGELMKAGMVSLQEANASLGEKLTLSEVARSEYEGVAKSLGKENDELRAKLAAVPSKEAVIAEYRESDVFKRAIVDARVARVEAYRGSEDFDSEMKSAVKRGVAVFKDSAEYADEMQRVRESAVDNYRSSIAFKQAVGAEAGKMSRQVVECCREFLKDDLQRPTQDFGGFFVAHVRRQRDGAPNAGGDASVSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAS2</accession>
    <name>A0AAF0WAS2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pirin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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        </source>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="127"/>
        </location>
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            <end position="285"/>
        </location>
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        <location>
            <position position="66"/>
        </location>
        <ligand>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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        <property type="project" value="TreeGrafter"/>
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            <end position="514"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="166"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="777"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG85758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="105893" checksum="7079756531BC04BF" modified="2024-05-29" version="1">MVGLSDIPCVGKNVDRISDAAVDALFRGFSYMFSYKNHVQVLETEIQKLDAEMDRMSRKVREEKDNGKMIVDGVSTWQADVEEVKKSAEEILEKYKNRSSWRCIQWMPIPKPVSRSRLGREAAHKAKRVIELSDSANNLLANGIAYGSPAENAPNTVYQTFKSRSDAYDKLWKALVSEGGSTVLGIYGMPGVGKTTMMESLWKEALEKKIFDKVTRADVGNEDLDVFKLQKQIADHLDCHLNPEDDEQHRASQLKKRLTNGEKILIILDDVWKEIPLHRIGISLGDGDSSTHRKILLTSRKKRVCMDNKCLHPVKIAPLGPEEAWQMFRNTVVPDNIYLLPDESLALEVCNQCGGLPLLIHAVGKALQFTSPDVWKDALNQLVMGNFEKIADIDSNVYACVKLSFDRLPDDAKSCLVLCSLYPEDASIYINKLIPLATGSRLVRGGEARIRSMVEILRSSSLLLDSEDHIIKVHDLIRDVARSIAVKDPKYSFSLVRCGSLLPDDVDYHTRKFLRLHLEKNDILFPDGLVCQDLHNLWLQCNKHAQQFLGDFFGMFVNLRFLLIEDMLFSLEPQFSLRPLVNLRTLILDGCEITHVSQTNASFFPENLVALYIWNCDLPRPLNLPILKHLRKLEIQGWRSNNIQMVPNTVSSMPSLQELHIPGGYKIQDDGCGKSLDAVSAPILGEISKLTGLKSLQMFFFSNSEHFQDTNIFGNLLQYNISVGAKPHDRPLIESSVSIKRLIELQGSGLESLKGLIERAEEVSLDCTDIHVGSIYNSNREAFTDLKNLYIRNCNTIEYLARISCGEIQHSRSFANLGVLEIKDCSALKYLFSKSVAKCLVQLQELAIVRCPVMEAILMNEGTSDRDGVISFCKLKFLSIYNIPRLKSFCMQNKDLHSGSTTHNSAISNIQAQPLFDKTVCFIYFIIIFNSNIIKICLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCW8</accession>
    <name>A0AAF0WCW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0105131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCW8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051958">
        <property type="entry name" value="Alba-like_NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516:SF3">
        <property type="entry name" value="ALBA DNA_RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="5">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG85938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="295" mass="32815" checksum="BEC4F35E7392465D" modified="2024-05-29" version="1">MEKYQKVQKPRPETPYNENEMRITSMGLVGNYVNYAASLFQERHGRKIVIKGMGQAISKTVAIAEILKRRIPELHQDTAIDSVSLVDVWEPIEEGLLPVETSRRVSAISITLSTEELSKDSPGYQAPTHVEQSRPRQNYQQQRFHQQPREARTGYNVAGEDAYGHGRVRGRGRGRGWNRGGYNNYDGNYRGNYQGGYQGNYQGGYHGNYQGGYQGNYPGVYPGYPGSYQGNYPGGYQGNYQENGGYSNRSQGGARGPRFSSYGYRVPCIISGLLTGTGYGRGRGRGGGRGRSHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WD49</accession>
    <name>A0AAF0WD49_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cellulose synthase</fullName>
            <ecNumber evidence="21">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0205838</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 21">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Cell membrane</location>
            <topology evidence="2 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="21"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WD49"/>
    <dbReference type="KEGG" id="dcr:108209273"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000009">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14569">
        <property type="entry name" value="zf-UDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 21" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="783"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="821"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="862"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="899"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="933"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulose synthase RING-type zinc finger" evidence="22">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF14569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOG86621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="973" mass="109695" checksum="2991B5CF54ED7282" modified="2024-05-29" version="1">MMHSGVPTCITCGEQVGLDHNGEVFVACHECSYPICKSCFEYDLKDGRSSCSRCGAPYDEISTMAVEETESVTQNTMATHLNISQEAGLHNRNISTVSTVDSEYIDESGNPIWKNRVESWKEKKNKKKKGATKVAKEAQIPPEQHMEETQQQNEALQPLSRIVPIPKSQITPYRMVIILRLIILGLFFHYRVTNPVESSYGLWLTSVICEIWFAVSWVLDQFPKWSPINRDTYIDRLSARFEREGEPSELAAVDFFVSTVDPLKEPPLITANTVLSILAVDYPVDKVSCYVSDDGAAMLSFESLVETADFARKWVPFCKKYAIEPRAPEFYFSQKIDYLKDKVQPSFVKERRAMKRDYEEYKVRVNALVAKAQKTPDEGWTMQDGTPWPGNNPRDHPGMIQVFLGHSGTHDIEGNELPRLVYVSREKRPGYQHHKKAGAENALVRVSAVLTNAPYILNLDCDHYVNNSKAVREAMCFLMDPQIGRDVCYVQFPQRFDGIDRSDRYANRNTVFFDVNMKGLDGIQGPVYVGTGCVFNRQALYGYGPLSLPALRKGSSSCCSCCFPKKPSKQLSEVQRDSKREDLNAAIFNLREIENYDEYERSLLISQMSFEKTFGMSSVFIESTLMEHGGVADSTNPATMINEAIHVISCGYEEKTAWGKEIGWIYGSVTEDILTGFKMHCRGWRSIYCMPLRPAFKGSAPINLSDRLHQVLRWALGSVEIFLSRHCPLWYGFGGGRLKWLQRLAYINTIVYPFTSLPLVAYCLLPAICLLTGKFIIPTLSNLATIWFLGLFFSIIATSVLELRWSGVSIEALWRNEQFWVIGGVSAHLFAVFQGFLKMLAGVDTNFTVTSKAADDGEFADLYVFKWTTVLIPPTTILVVNLVGVVAGFSDALNKGYEAWGPLFGKVFFAFWVILHLYPFLKGLMGRQNRTPTIVILWSVLLASVFSLVWVKIDPFVSKGDSTSTQACIAIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDX4</accession>
    <name>A0AAF0WDX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0207046</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDX4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06921">
        <property type="entry name" value="ChtBD1_GH19_hevein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000001">
        <property type="entry name" value="Basic endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF79">
        <property type="entry name" value="CHITINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00451">
        <property type="entry name" value="CHITINBINDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 12" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="9 11 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042054786" description="Chitin-binding type-1 domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="14">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="101"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="168"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG87814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="33535" checksum="B93299F50F53A74B" modified="2024-05-29" version="1">MRFWIVTVCIFALSFASSAEQCGKQAGNALCPNGMCCSEFGWCGTTPDYCSDTCQSQCGGSTPAPGGGSTPTPSGGSGDVSSIIPESLFNDMLKHRNDGSCKSNGFYTYSAFINAAQSFDGFGTTGSTDQRKQELAAFLAQTSHETTDGPYSWGYCFIAERNNPGTFCTSPDWPCASGKEYYGRGPIQLTHNYNYGQAGKAIGADLINNPDLVATDAVISFKTAIWFWMTPQSNKPSSHDVITGKWSPSADDNAANRVPGFGVITNIINGGLECNKGTDSRVEDRIGFYKRYCDILGVGYGNNLDCNSQRPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WEJ1</accession>
    <name>A0AAF0WEJ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0207139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WEJ1"/>
    <dbReference type="KEGG" id="dcr:108207558"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF196">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="27"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="170" mass="18793" checksum="122F930A31FCB106" modified="2024-05-29" version="1">MKRTHEERENFECHENSSEQRISYDLVPAPNVTGLMRRIIPNQGEISADAVISIQQCVIKFIRYVTAEANTRCGEGMRTTITAHDVLIALNKLGFHHYIGPLFIYMNRLQEFQPEQGDPLVIRRTLFQPRGMGIGGPGFDVEGVMTMGGFVGDDQNDAPSGSAAHSTGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFB9</accession>
    <name>A0AAF0WFB9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0206932</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFB9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="16178" checksum="5CC60307BC62DBD3" modified="2024-05-29" version="1">MCQIMHTTMGDVHMKLYPEECPKTVENFTTHCRNGYYDSLIFHRVIRGFMVQTGDPLGDGTGGQSIWGRDLRHDRPFTVSMANAGPNTNGSQFFITTVATPWLDNKHTVFGRVVKGMDVVQVCTCIFYKPYDDIKILNVTVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFJ5</accession>
    <name>A0AAF0WFJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207852</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Bowman M."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Iovene M."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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    </reference>
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</entry>
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                <person name="Iovene M."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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    </dbReference>
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    </dbReference>
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            <begin position="42"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG90863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="369" mass="42713" checksum="4F8D92D50D20F3C1" modified="2024-05-29" version="1">MVEINVEDEDEDWDEDEEHYCYHERYDYRKNSVSETRGLQRLATGKTYDVELPEASGRLILGTNKGWLLTLGRDLQISLLHPLLRQDIPLPHMGTFLHQPHSEEFILYVSPEQAPEVFIQRVAMSCKLHPSKNNGMYSSNPIVMAIYGARRYLAYARLTDKVWTEVFFPIMAPFIEDIAYYKGKFYALNGRGDLFVCDINDDSETQGRAKGTKIYSWPTDLDIGMNYNNSRTYLVESGFGFWLVVREFKAKYFKAPHGARVKYRTCNFTLWKMELKYSDHHSELPSCTCIPENNLGDQALFIGRATCLSLPSSEYIRPNCIYFTDDNLDVFYHVGGGHDMGIFNMETHTIEPFFHGKSIHPISPPLWYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WND1</accession>
    <name>A0AAF0WND1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0311567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="263"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG92304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="637" mass="74135" checksum="9C4386A63DF2EB22" modified="2024-05-29" version="1">MQLQFPLLFPQGDDGYHTEIPLKNCKKRRPEPADNDETEGDRKFREHVTMKEYYSYKLMIRTNEGSFLFLLPLHCHKFLTNINHQSCILYNRLTPHLGGRLWQQYVVDAFTAMEQYRLDWISRNQTTIRSDLYNSVRDAVRRGDNDPSHVGKCVILPASFTGSKRYMSQYFKDSLALCRSIGHPSLFLTMTTNSKWPEITEMMKHLPGVNVADAPDVVARVFKMKLDQLIDLIKKKHFFGRCIGLMHVIEFQKRGLPHAHMLVWLDDKDKPKTAEQIDKLVSAEIPDEESDPICYQAVKNYMIHDTGKTVKRKGVLLNNRFVVPYNRELLLRFQCHINLEICNNSRSLKYLFKYCLKGHDTATMLLKRKNDKSSAVDCQERKGKIFDEVRHYLDGRYVSASEAAWRILGFGIHSRWPSVDRLPIHLPDSKYVSFKTGESLQNVCHRADSKRSKLEAWFLANKLLPDAKNYTYNEFPSYFTWLPKECKWKYRQRGDVVGRLSEVHATAGDLLYLRMLLMRKKGCLGFEDIRTVDGVVYDTFKEACGAMGLLQDDNQWHEALSENSHSAFPHQLREMFVNILAHNTVADPLLLWTRHWKCMSEDILRKQRSTTGINDLELPEVDIHNYTLAGILVFTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108212898"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="140" mass="16296" checksum="843701E091EEA1EB" modified="2024-05-29" version="1">MAGNYQLALEERSSWLQTENIWSSRQHQKSLWSPTTRESPNSELNSKPQLRQIYHKDWAAWRNEMQTISLDSPKKSCGTGFFLPRSAETDFQITNKSDTKRSSENWNNNVAGNKKSQRSAASYKTSSSPHYIYLPKEWTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DCAR_0314237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRY7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF80">
        <property type="entry name" value="HTH MYB-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="264"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="439"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG94940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="488" mass="53359" checksum="AB1CE9F68418B272" modified="2024-05-29" version="1">MEARHALSFQTSSARPLSNHGAPGGLSSSLPVLSNHQLPVYQEASMERTLASNSSNVRPLLPSNSGVVGHIISSSAGFSSDLQYSTSASHGNHSSKTPFISQSSNHGATMPLHNHSDSGIFKSTASSRCITENNNDSWCTDSLPSYLDCPVTSPGANNQIDDKNGVCVVPSEDFNKTSDWQEWAEQLITDDNALPPNWVDLLVDDTSVAGPEQQIIPYQMSTTPMNCPVQQPQVHQQQQVSLQLPASSEETSTGVTKSPSAGGSSSKARMRWTPELHEAFVEAVNKLGGSERATPKGVLKQMKVEGLTIYHVKSHLQKYRTARYKPDSVEGSSERKLMPIEELSSLDLKTGIEITEALRLQMEVQKRLHEQLEIQRNLQLRIEEQGKQLQMMFEKQQKSGLDMAKVLLTDMDKPEAVQESPAKSEQGSLLLQHQLPVDVPENAAPASEDISAHLTENHSEPEVEISKDLASTVTDNSTSQPAKRLKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTE8</accession>
    <name>A0AAF0WTE8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0414776</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTE8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF52">
        <property type="entry name" value="LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="486"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG95457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="548" mass="62612" checksum="35B66E1AD0D645F4" modified="2024-05-29" version="1">MENKDLSTPFLPSQSHSKYDNDEPQFIITVHDPEAQKVPNGLNFKNLDDPFEFLGSKGYEMLDSTTVDPFRNGTYSIEGVYEWLKIGICLPIALVRMVLFGVCLSVGYVATKIALLGWKDKENPLPRWRCRIMWVTRFATRGILFAFGYHWIKRRGKPAPRDTAPVVVSNHVSYVDPIFFFYEIFPTIVASDSHDSMPVVGTIIRAMQVIYVNRFSPSSRKHAISEIKRKASSNRFPRVLLFPEGTTTNGRSLISFQLGAFIPGYAIQPVVVRYPHVHFDQSWGNISLARLMFRMFTQFHNFMEVEYLPVVMPLVGQKENAVQFCKRTSHAMCVALNVVQTSHSFGDVMLLAKANESKQVNPSLYMVEMEKVAKTYHISSSEAVEFLDKFLSMKPDLSGHVGVCNFLRILRLKRCYFSEKVFGFIDMKKTGRITFKEFLLGSAHVLKHPLFRRACELAFTECDISGNHNISEQEFGCSIIMAMQNLKEDEIHELFILFDFNSDGRICRDDFMACLRQNPLLISLFAPRLLQLDLSSKVCERTTESMGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY14</accession>
    <name>A0AAF0WY14_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0416320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY14"/>
    <dbReference type="KEGG" id="dcr:108216751"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF9">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG96981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="434" mass="48555" checksum="1D0B6601215E0424" modified="2024-05-29" version="1">MILLSPKPTCILFNKPINPFSKSNHPKPHHQNNTTPSSNHHISCKSEYKTERGLQFDVGDTFFRHESATGRDLAVLAAAIHKKANNDKLRVLDAMCGCGIRSLRYLVEADADFVLANDADDFHRSVVLSNLSAKVPRISEDGRGRRWLVTNDDANRVMMECCLRRDYFDFIDIDSFGSDSSFLRSAFEALRFGGLVYVTSTDGYTSGGHRPLHSLSSYGAYICPMPYCNELGLRMLIGGAIREAAVLGYYVTPLFSYYSYHGPVFRVLLRVNRGKLPISRHYNFIRYCSECGNSEAISWDKLGQLNCPCNSNVPGSLVVSGPLWTGPLHSKSYLEEMLNLAEEWGWIGNNIGVDLEKLLKYMIDESDPQLPFGYIKLDEVSSRGKINSPPLRSMLSSLQKNGYTASRSHIESNAIKTNCPMSMCIMIAKELQAC</sequence>
</entry>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="entry name" value="At1g75730-like"/>
    </dbReference>
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        <property type="entry name" value="OS02G0121500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS02G0121500 PROTEIN"/>
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    </dbReference>
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        </location>
    </feature>
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        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="645" mass="71188" checksum="2837CD1FC40A9CA8" modified="2024-05-29" version="1">MDKTPDVRRAKPSRSSSNVTAQSGSDFKKLRKDKVRRLSFVDRLCDKTPDSEMPCVQSDDEEDEYHPCVTKSKRIKLPRKFFDDCQKVDHSSVPRKLRSAIRKRRRESISPHFPEQPKESEGSPGLASVMPVSKDEEEVAETLNALAEMFSDSDKIEMAKSTGKLCNAKSYDSQTKSSYLYSNISFINTVIKTAAPEVEKDLRSPFTTTAPGAAQHSSNTVGSADEAERIQFSYRRTEADMHRYIPKFNCTKVPILSKSSSANENCSSFPSHSQLSREIGLVPFACSALWPGLSSTSVHSAKTLGSPVKLSAAKVPTWLGISTSTARSCSSRNVAISEKEFQSVVDEKKMRKKCSTHVYISRLIRVLKVTEAKDSLPLQPTRLTAHDESKKGAISEEKKLQMIKEGSAGVVFTSDIACSGGEDSPSKLSSAIVLHKRLRDDLQGSTNSGPYTTHKQSSNFFAALPGSSGVEHESAGQVNGHRPEPSTESNIPFLYPFTQSCRTNPLSLPQNCQLSTSLVDSAAAGAAPKIQLPPYLSNTKSVTSSLMSLQNQQQHQQWLWAAQLTAPRKPEEFAAAASHVPNWQNSRQEFSHMQYGQLIHPAALEVLGPRYIPVPTQQQHLMSSTSLFPQSRVQRYNHQLSTKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0418035</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
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        <property type="protein sequence ID" value="WOG98691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108215782"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TBL"/>
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        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF71">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
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            <begin position="1"/>
            <end position="26"/>
        </location>
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            <end position="364"/>
        </location>
    </feature>
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        </location>
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    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="364" mass="41908" checksum="8EAB5A161DFE7AC0" modified="2024-05-29" version="1">MGTSLFKFMVFSALFVFICCQQNTNAEEDFVSSNSSHIRKLASGCNIFRGKWVYDASYPLYQASSCPFIDQQFNCQKFGRPDQSYLKYRWQPFSCNLPRFNALAFLEKMRGKKILFVGDSLSFNIWQSLSCMIHSAVPQVKYTIVQGYPHSEITFVDYGVKLLLYRTTYIVDIVNEQKGRILKLDSIKSGDAWLGMDVLVFNSWHWWVHTGRDQPWDYVQDGAHVYKDMNRLVAFYKGMTTWARWVNRNVNPSKTKVFFQGISPIHYEGKDWGEPSKSCAHETEPFFGVKYPAGTPMAAVVVNKVLNRIKVPTYLLDITLLSQYRKDAHPTVYSGGRNSLDCSHWCLPGLPDTWNQLLYTALVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4W6</accession>
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        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
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        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X4W6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044:SF29">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH01653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="50540" checksum="EC4C2E418B82E40E" modified="2024-05-29" version="1">MAETKKSKLRVLMSPFLGYGHIMPFVELAKKLSQTNDFCSYICSTPANLNSIRTKLSTSDLSSSIQLVDLHLQSSPELPPHYHTTNGLPEHLHVNLYKAFKMSQPDFFEIMKNTKPHLVLYDAFQPWVPDMASSLGIPGVYFHISSAVSTAFFYSFVKICIRCEEPFPFPAIFLRPGEIKRMLATTPVDPTVLDPVGPCLDKSKDYILTKTCNDIEDKYLDYLSKLTNKKVIPVGLLNDQRKQQSDDSCAEMINWLDSKENASTVYISFGSETFLSNKDITELASGLEMSGTNFLWFIRFPSDDGKKIEEVLAQGFIDRVKGRGMILQGWSPQQKILGHASVGVFLGHSGWSSVIESLSFGVPIIGLPMKSDQPLIARLLVEHGVALEVERDNQSLDFTREEVAKVIKEVMQSGSSVRMKAKKLSEKIKINEDQVLAAAVEKLKNICTGNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X6B7</accession>
    <name>A0AAF0X6B7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0521566</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X6B7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="43"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH02178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="377" mass="43162" checksum="EFC319E32D1B0146" modified="2024-05-29" version="1">MGNEDSSDSQVKVKVEKGKPTHGGRYVEYNVMGNLFHLSSIYAPPVQLLGCGSYGVVCCATNSETKEEVAIKKIANAFDNRIDAKRTLREIKLLCHMDHENVIKIKDIIPPPEKENFVDVYIVYELMDTDLHQVIRSSQALTEDHCQYFMYQLLRGLKYIHSANVLHRDLKPSNLLLNSNCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILMEIIKREPLFPGKDYVEQLLLITELLGSPEDSDLGFLRSNNARKYIKQLPHVPKKSFAEKFPDVSPVAIDLAEQMLVFDPSKRITVEEALNHPFLSSLHEINEEPTCESPFVFDFEQANLSEEDVKELIRLECQKFNPDKMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8F1</accession>
    <name>A0AAF0X8F1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0521186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8F1"/>
    <dbReference type="KEGG" id="dcr:108220359"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007142">
        <property type="term" value="P:male meiosis II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039300">
        <property type="entry name" value="JASON"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318">
        <property type="entry name" value="ASPARTYL/GLUTAMYL-TRNA(ASN/GLN) AMIDOTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318:SF4">
        <property type="entry name" value="OS04G0511700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH01801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="51596" checksum="3906EE139FBDDC76" modified="2024-05-29" version="1">MGCFLGCFGSSKDKKRKKQRRYGSPRLQRIGSQTLVQTSTTETPVLSIPEKVAYSVLESCDEKSEEQLSLSTRKKVTFDTNVQTYEHVTVYGSTDLLLDKNDVSEKEMEEEGDKKDGLARSSQSRTSSEDNSTISSVGSYPQNHRYNNCRDSDDEAVESEYEDSDLDDEEEDEYGDDDYDDEINDGSVLCQEVWSETIPVESKESRTGVSLKRVGSEEVDSPVPCVMPEQEVKTLLLNRNARDRSVYVHPVLNPVENLSQWKAVKSKGTSLLNPHQKENSFADGEAPRISLSTEPSFKQSSYSSKPQSNYQTKNQNKEMAVDASLSNWLVTPEITPDKKSSSIKLQPVNSEKSMSQGSNSVMSIEDRPILGALTLEELNQFSATSSSPRKSPSRSPDEMVLMGTVGTYWKHTGEAKNRGSASSYKGIPNTTSKYREDKKVNWHSTPFETRLERALNRGSAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAK1</accession>
    <name>A0AAF0XAK1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0622877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAK1"/>
    <dbReference type="KEGG" id="dcr:108225644"/>
    <dbReference type="OMA" id="HAGYWIH"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="182"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="284"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="56310" checksum="654C36D3F0A144B2" modified="2024-05-29" version="1">MGRRIIKQHHMIQAIRNSYDLTNLLQQCKVSDISKVHSQIVTGGYGRNPFVGTKLIGRYSESASPNMEHARKVFDGLSDRDVFLWNMIIQGYANSGPSVEAVNMYKELCRSSVLPNQYTFPFVFKACAAMRDQIAGILVHAHAIKLGFGFNLFVGNALVAFYAKCQNIDASRCAFDQISPKDLVSWNSIISGYTTNGYYHEALVLFHAIQKSVDCKPDNATLVAILPACTQLASVQDGLWIHTYILKRGMEINAALGSGLIAMYGNCGRLNDARWIFDRILNKTVVVWNAIIRCYGMHGHADEAVKLFSRMIEDGVCPDSRIFLGLLSTCSHAGMVTRGRELFESMGDYGVERSNEHYACMVDLLGRAGFLKEAMELIEAMPLQPGKDVYGALLGACRIHNNMELAEKVAKKLFVLDPENAARLVILANMYEDKGRLADAAMARKMVREKKLKKSAGYSSVELDFVYHTFGVEDESHPFTELIFDTLEKLYQVMVMEDDLTLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAV3</accession>
    <name>A0AAF0XAV3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Probable DNA helicase MCM9</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Minichromosome maintenance 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0624313</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Probable DNA helicase that may play a role in DNA repair during meiosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAV3"/>
    <dbReference type="KEGG" id="dcr:108225712"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17760">
        <property type="entry name" value="MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002270">
        <property type="entry name" value="Probable DNA helicase MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058768">
        <property type="entry name" value="MCM9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF48">
        <property type="entry name" value="DNA HELICASE MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26066">
        <property type="entry name" value="MCM9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="650" mass="72156" checksum="2ADA0895863F2383" modified="2024-05-29" version="1">MEDEQENIRGFLVDHCSDQLSSILLSPDTRLHYPLLLEFAELLDSNPKLANSVFNQPALYLPKFDKGAKEAQEILRKELKSLRNASVKDYVHVRINICGSPLEFPETFPSIGRVRVKHRGILLTLKGTVIRSGAIKMVEGDRTFECRRCKHRFKVSPELETGNSVPKPTFCPSEKQKYCESTSFKLLEENIVCHDYQEIKIQESTQVLDVGAIPRSIPVILKDDLVDIVKAGDDVIVTGILTARWSSDMKDVRCDLDPILVANHMRRTNELKSDVNIPDDTIMEFKKFWSEFRDTPLKGRNAILKAICPQIFGLFTVKLAVALTLIGGVQHVDASGTKVRGESHLLLVGDPGTGKSQFLKFAAKLSNRSVITTGLGSTSAGLTVTAVKDAGEWMLEAGALVLADGGLCCIDEFDSMREHDRATIHEAMEQQTISVAKAGLVTTLSTRTIVFGATNPKGHYDPELSLSVNTTLSGPLISRFDIVLVLLDTKNPEWDAIVSSHILAEAVAEKGSDENLQGIWTLTMLRRYLHYVKASFKPVLTKEAEKVISSYYQLQRKSAAQNAARTTVRMLESLIRLAQAHARLMFRNEVIQQDAITAILCIESSMTTSAIVDSVGNALHSNFTENPDQEYVKQERTILKKLACGEEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XC95</accession>
    <name>A0AAF0XC95_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0623861</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="358" mass="41048" checksum="A07CB81CC0732ECC" modified="2024-05-29" version="1">MTIASSTMSQNHHLDHKDDDEDEHEHDMVMPGFRFHPTEEELVEFYLRRMVEGKRFNVELITFLDLYRYDPWELPAFAAIGEKEWFFYVPRDKKYRNGDRPNRVTTSGYWKATGADRMIRTENLRSIGLKKTLVFYTGKAPKGIRTSWIMNEYRLPHHETERLQKAEISLCRVYKRPGVEDHPSLPRILPTRASSSRTNPSTKRHDRDPPAQHHAMEKLQVFGDQTVQQTSQKLSDTISADSTTEDIGTTSLCLSSQNPSSSLLINSPNTIHDFHTLISYHQASVNQPHQFFPDTLQLSANNVLQPAQQLQSLTLAVFPAESAQSAYSDRVMWDWNSIPEATASRDHGAFLGRFSGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCB6</accession>
    <name>A0AAF0XCB6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0623090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCB6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8500" checksum="7EB24212E5ED7E67" modified="2024-05-29" version="1">MKNINFQAIQNVVAAIWRPKEGIEKVIDGGPWTFEQSLLVYHKLEGYEDAQLIKLNNVEIWVQVYDLPSGILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFW9</accession>
    <name>A0AAF0XFW9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0625061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJR7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF57">
        <property type="entry name" value="XYLOGLUCAN O-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="10">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="9">
        <location>
            <begin position="137"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH08337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="50654" checksum="6273982AC6F75F99" modified="2024-05-29" version="1">MGSSSPLKDHYFSSFNRSKLITWSLYTLVSFAFIRFYFYPYFSLVSTQDQFLTTNSRVISTTSTPSSSSSSSTSHEKPHVESTTCDYTNGEWVQDKQGPMYNGTSCGTIKKGQNCMRHGRPDTGYLYWRWKPKDCQVPRFEPTIFLDMLKNKHVAFVGDSMARNQLESLLCMLSTFSAPNLVYRNGEDDKFRRWNFPSHNVTVSVYWSPFLVKGIEKSTNGGPDYNRLFLDSVDERWASDLGSIDMVVLSIGHWFLHPAAYFDGDKVLGCHVCSGMNYTEIGFYGVYAKAMETTLKAIMKRRGSRVGSDSNGAIDVIVTTFTPAHFEGEWDKPGACSKTKPYKEGEIELINMDAEMRKSELDVVKVAKKKAKRFKNLRLEAVDVTKLALMRPDGHPGPYMNPHPFANGVGERVQNDCVHWCLPGPIDTWNEILLDVIKRWNGQSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRB6</accession>
    <name>A0AAF0XRB6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UV-B-induced protein At3g17800, chloroplastic-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0831048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRB6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038925">
        <property type="entry name" value="At3g17800-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808:SF2">
        <property type="entry name" value="OS04G0596300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH11559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44312" checksum="EBAA9352FD6C4A71" modified="2024-05-29" version="1">MQISSSVAGDVSLVLSSTTHTSRSLLFPYLSPATLKRCPSSFSLKLGVGSYTTRARTLTVKASGDPSPDSSRLAPLELESPVGQLLAQILHTHPHLLPAAIDQQLENLTNERETQREEATSSQDLALYRRIAELKEKERQMAVEEIIYCLVVQKFLDSGISLIQKVSATSDPVGRVDFWPNQEDKLKSVHSPEAFEMIESHLSLVLGDRVVGPVNDIVQISKLKLGKLYAASIMYGYFLKRVDERFQLEQSMNTLPEGFKEEQPTFQQPEPQSPFWDPDSLIRLMPDDGDGGSLDSLEDKSYRLRSYVMYLDAETLQRYATIRSKEAISLIEKQTQALFGRPDIRIAEDGSLDTSSDEVVSVTFSGLTMLVLEAVAFGSFLWDAESYAESKYHFTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSW8</accession>
    <name>A0AAF0XSW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0831721</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSW8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="7">
        <location>
            <begin position="218"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="403" mass="46551" checksum="F55FA8210294F02A" modified="2024-05-29" version="1">MGLLRLCNHSVRFSSVLRFPYYPSTLTSLQRVTSLASAKICDGPENLLSGFELDQKFCNALRHFVGTKQYSVHRLLGSGFDQELRKPSRHFVGTRYYSVSKIQKYVYRPLVKCLSYVTFFIGKFEITPYTNITLYVFPFFDKIYGEILFSSLKQDCGRGLRLPLTDPRCIRVESIATKILEALQRETGRRHVLADQQWIKDDVDGKVKLVKRSARTEESTNVHLKEGINWEVFVIDQPIIQAGVTGSAANMGKIVLFTGLFDVYKSDEELAIIIAHEVGHVISRHIQRRFLWLPFKKIYHRVINGYDSGMHYHLRYEHEADYIAMLLSASAGYDPRFAPPAISIMEKYLTLEFVKYNDLPFAKLESAERFEFMTNPEVMQEAVRIYQEKTKGQCADQEYLAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XT71</accession>
    <name>A0AAF0XT71_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0933309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    </dbReference>
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        <location>
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            <end position="314"/>
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</entry>
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    <name>A0AAF0XUV3_DAUCS</name>
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        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
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    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:57766"/>
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        </reaction>
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        <text evidence="3 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
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        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
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    </comment>
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        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
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        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH12826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="28797" checksum="E22341438B92CDC7" modified="2024-05-29" version="1">MKLLSVPPHTFLTNSSPKIRVRVSQSPLISHQNLTFSPIQNPKTRTRICSMPQNNGPSSFQLQTDVVRIGEVKRVTKETNVAVKINLDGSGIADSSTGIPFLDHMLDQLASHGLFDVHVKATGDIHIDDHHTNEDVALAIGTALLNALGDRKGINRFGDFSAPLDEALVHVSLDLSGRPHLGYDLSIPTARVGTYDTQLVEHFFQSLVNTSGMTLHIRQLAGNNSHHIIEATFKAFARALRQATESDPRRHGTVPSSKGVLSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XV28</accession>
    <name>A0AAF0XV28_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0933385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XV28"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="208"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38534" checksum="02340160ADA8010F" modified="2024-05-29" version="1">MYNTIIDGLCKTSNTSMALKLLRKMEEIGCNPDVVTYTSIIDGLCKTSNTSMALKLLRKMEEIGCRPNIVTYSSIIDSLCKERRVDHALVLVSEMTDKGISPNVITYSTLLQGLCSSGRWEDTESLLTEMGVRKISPNLHTYNILVDAYCKEGRTTDAEDVIEIMIQKGVPPDSITYNALMDGYCLTGKMDRALEVLNTMRSNEIWPDCYSYTILIDGLCKNKKLDEARNIFDKLASRGLQPGVIAHNIMIRGLCQEGLFEDAKELFSKMETSICLPDDVTYNTIIRGSLLNKRYEEAAVLIENMRARKFSEDASTRSMVLDLLSKEEQDPSVLAFCKWFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVB8</accession>
    <name>A0AAF0XVB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0833004</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVB8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF80">
        <property type="entry name" value="CHROMATIN REGULATOR PHD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="8">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="9">
        <location>
            <begin position="284"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH13494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44009" checksum="8CAECDED1C4FA60F" modified="2024-05-29" version="1">MGAIIILAELGVKELQYVLSMLGLEYHGSERDLRARILDTLVSEQVLLYHSTSNNSMLKKIACFSLYFLSCENPHCRVLQHKTCALGLGPAPPHYYCQVCRINRADPYYLLIMVNPLHIQKFTNQNKLNTSNLALSIVRQDVNFSSKLEMVAEVMYLINHKGYEVQACCIFLDDVDQFRMRWPSNSKIKVNGNDVKTEGRLGPGGLRDGVLISTYLDEGSNDISFTASPNGIFRLGIRLVKPRTIQEVCSIICNKQDGELLNDATARVIRCVTGGAATLDAAQRELEVLADSAAVDLRCPISGGRMKIASRFRSCIHMACFDLESFLGLAKTTKKIEQSGADTAEIKIRADGSWFPSNEASTSRTNSITCDDLSSPQRTPTNHGASLDDPLILKIPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XW33</accession>
    <name>A0AAF0XW33_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0934807</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XW33"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH15270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="47896" checksum="3BA7EF80118B0E82" modified="2024-05-29" version="1">MFYASLCLMALGAGGLKGSLPALGADQFDAKDPKGAQSLASYFSWYQLSVTFGSIIGVTGVVYVSMNVEWYWGFFIGLVAAFVGFVVLALGKPYYCIQPLASSPIIKIFQVIAVTFKNRNLTLPEDHMNLYEIEDKERDISDVKILHTDQFSILDKAAIIPEGVKPEAWKVCTVTQVEEVKILTRMLPIIGSTIIMNTCLAQLQTFSVIQGIYTDPHLGSLKIPTASIPVIPLLFMSILLPIYEFIIVPFARRFTGHPNGITQLQRVGVGLVLSVISMAIAGLIEVKRRNQFYDNPLKPISLFWLAFQYGVFGIADMFAMVGLMEFFYKEAPSGMRSLSTSFALISLSFGYFLSTAFVSIINAVTKKITPSKQGWLHGQDINHNNLNLFYWFLAIISALNFVSYIYCAKWYKCKEETKVAASETEEKVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XW68</accession>
    <name>A0AAF0XW68_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Galectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0833071</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XW68"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990714">
        <property type="term" value="F:hydroxyproline O-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000005">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF286">
        <property type="entry name" value="HYDROXYPROLINE O-GALACTOSYLTRANSFERASE GALT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galectin" evidence="13">
        <location>
            <begin position="138"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH13561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="629" mass="71950" checksum="105832BFC3801197" modified="2024-05-29" version="1">MKLYKCDISSSSNRFKLILVCVLFMYLLVITLEIPHLSIGFVDNVFVDRSFHRSPKRHVKSVKRVSGLVLDENGFVGIEKNRNFSELVEMAREAIVVGKKKWEDIVAGKVKIKKKGNGTVECPKSLLLSELEFRKKGGMVLMQCGLGVGSYISVVGKPRKAHLEKNPKIGAKRGEESAMVSQFVIELQGLKVVDGEEPPKILHYNPRLRGDWSGDQVIEQNTCYRKQWGASLRCVGTKSKASEETVDGQVKCENWIQRHGNLSQKANTSWWRNRLKKLIDKIYRKRPFPFVEDKIFVLTLYAGMEGFHVSVDGRHVSSFPYRPGFTLEDATGLSIKEDVSVHSVFATSLPTSHPSFAPLGLLEMVPKWKAPPLFDSPVEFFIGIISAGNHFAERMAVRKSWMQHKSIKSSNVVARFFVAMHKKKDVNAVLMKEAHYFGDIVVVPYMDNYDLVVLKTLAICEYGVRTVAAKYIMKCDDDTFVRLDAVVKEVKKAREDKSLYLGNINYNFKPFRRGKWAVTYEEWPELSYPPYANGPGYIISSDIASYVLSRFEKHKLRLFKMEDVSMGMWVNKFNKSRPVEYVHNLKFCQFGCIEDYFTAHYQTPRQMACLWAKLKHRGKPQCCNTRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XX25</accession>
    <name>A0AAF0XX25_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0933542</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XX25"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH14026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9706" checksum="7DF21CE1D99D481C" modified="2024-05-29" version="1">MSVKNNTQVLIFLLFSSRYNKKLLGRVRAFDQHYNMVLENVREMWTEHPKADKVKKKSLPVNKDRFISKMFLRGDSVVSFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ADW0</accession>
    <name>A0AAF1ADW0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Subtilisin-like protease SBT1.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0100339</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ADW0"/>
    <dbReference type="KEGG" id="dcr:108204266"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="131"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="9">
        <location>
            <begin position="374"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="11">
        <location>
            <begin position="660"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG81194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="770" mass="83701" checksum="3EF088404E0093E1" modified="2024-05-29" version="1">MEFEKATLFAILLVYVVSVGSHGLQTYIIQLHPQGITRSLFASKLHWHLSFLEKSISAEESPSSRLLYSYHSAMEGFAAQLLESEVDAIRELHDVVAIRPDRRHEVHTTYSYKFLGLSPAKEGAWVDSNYGRGAIIGVLDTGVWPESPSFSDHGMPPVPKKWKGICQLGEQFNSSNCNRKIIGARYFSKGHRVASPTSSPDVVVEYLSVRDSHGHGTHTASTAAGAAVPMANVLGNGAGVARGMAPGAHIAVYKVCWFSGCYSSDILAAMDVAIRDGVDVLSLSLGGFPIPLYEDSIAIGSFRAMEHGISVICAAGNNGPIPNSVANEAPWIATVGASTLDRRFPAIVRLSNGKFLYGESMYPGKNSASAAKELELVYHADNLHGGEYCMKDSLPRAKVQGKMVVCDRGINGRAEKGQIVKEAGGAAMILANTEINQEEDSVDAHVLPATLIGYTESVQLKKYINSTRKPKAQIIFGGTSIGKSRAPAVAQFSSRGPSFMDPSILKPDMIAPGVNIIAAWPQNLGPAGIPDDSRRVNFTVMSGTSMACPHVGGIAALIHSAHPKWTPAAIKSALMTTAYITDHSGKQIMDGDKPAGLFATGAGHVNPVKAISPGLIYDISPDDYVTHLCTLKYTKSDIFTITHRNVSCQDIIRKNRDFSLNYPSISVVFRPGMKSKMIKKRLTNVGSPNSIYSLEVKPPNGIKVRVRPRRLIFTDINQSLSYRIWFISRNRTALERLSNSQGYLSWVNSQDIYQRIRSPISVTWSSKKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIZ1</accession>
    <name>A0AAF1AIZ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Beta-amyrin 28-oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0101104</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIZ1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF53">
        <property type="entry name" value="BETA-AMYRIN 28-OXIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG81946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="54540" checksum="6FCC279752E93F66" modified="2024-05-29" version="1">MDRLEFLSLSALVAIVVSLFLFFFSFRRSKANVPPGSSGWPVMGESIKFFRLGPQEFIKERTRKYSQDVFQTSLFGQKMAVFCGPIGNKFVYANETRSLAAWWPLSVRKALFFPEFVESPTDEISSLMFISIRDILKPEALKQYIPVMDSMAREHVDSQWADNEVVKVFPVSKKYTFDLACRILMGVVDAELVIRLAKHFTLVTAGLFSVPIDLPGTPYNKGIKGGKLVRAELMKIITERRKEMSENKSETVRRSNFLSRLLLVTDENGKFASEREITNNIVGLLFASYETTSSAVTFVLKYLSELPHIYDEVYKEQMEIAKSKSEGELLTWEDVQKMRYSWNVVCETLRFIPPLLGGFRDVVNDFNYAGFTIPKGWKTLWTVHTTHKNPKYFADPETFDPSRFEGRGPAPSTYVPFGGGSRMCPGKEYARLEILVFLHNIVTKFKLEKVNPQEKIELYFLPVPKEGLPLRLTRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJ91</accession>
    <name>A0AAF1AJ91_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase II protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0103767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJ91"/>
    <dbReference type="OMA" id="TCAICEN"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54021" checksum="EAD12D9FFA943F9F" modified="2024-05-29" version="1">MNKKNTSYCCALCDNSNVPSICSTCVNYRLNENYTNLKALKARRDQLYSRLTQLLVAKGKADDQHSWRVHQHEKLATLRQKLHHRKEQLLQGKSNVKKMTYDIKVKYEVLESAMSMLEKNRKEQLEKYYPNLICTQSLGLMAISSERLHKQSVVVKQICKLFPQRRVLEGERKDGVSGQCDQICNAHLPRGLDPHSVRSDDLAASLGYMVQVLNLVIHNVGAPALHNSGFAGSSSRIWQRDSYWNARPSSRSNEYPLFIPRQNFCSTGGETSWSDRSSSNFGVASMESEQRPNLDSPGRNSFNYSSASPHSVESHMDLQKGISLLKKSVACVTAYCYNTLSLEVPAEASTFEAFAKLLATLSSSKEVRSAFSMKMAGSRSHKQVQQLNKSVWNVNSPISSSTLLDSTYILPRSKNMFDKTSPSSANSFLYSAALSDSGKGENLIEGWDLVEHPTFPPPPSEIEDVEHWTRAMIIDATKYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKF8</accession>
    <name>A0AAF1AKF8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0102590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKF8"/>
    <dbReference type="KEGG" id="dcr:108204457"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="252"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="907"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="650"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1111" mass="126743" checksum="3602695105333082" modified="2024-05-29" version="1">MTTEQLTPGGGGPRYVQMQPEPGTSMMSSFFSFRQSQPESTRIFDELPKATIIQVSRPDASDISPMLLSYTIEFRYKQFKWQLHKKAAQVFYLHFTLKKRAFFEEIQEKQEQVKEWLQNLGIGENTTMVHDEDEHDDETVPLNHDGSVRNRDVPSSAALPIIRPELGRQHSMSDRARVAMNGYLNHFLGNLDIVNSREVCKFLEVSTLSFSPEYGPKQKEDYVMVNHLPTIHNDDDHKKCSPCQWFNCCNDNWQKVWAVLKPGFLALLKDPFDTKPLDIIVFDVLPASDGIEGARATLAVEMKERNPLRHSFKVSCGNRSIRLRSKSSAKVKEWVTAINDAALRLPEGWCHPHRFGSFAPPRGLAEDGSEVQWFVDGRAAFESIASAIDGAKSEIFICGWWVCPELYLRRPFQTNASSRLDALLEAKAKQGVQIYILLYKEVALALKINSVYSKRKLLEIHENVRVLRYPDHFSSGVYLWSHHEKLVIIDNHICFIGGLDLCFGRYDSSDHKVGDVPPNIWPGKDYYNPRESEPNTWEDTMKDEINREKYPRMPWHDVHCALWGPPCRDVARHFVQRWNYAKRNKAPNEQTIPLLMPQHHMVIPHYMGSSSEMEVEKKIVEDIYSDMKRNDSVASLSSFQDIPLLIPQEADELRPSDGDLKSNGLEMKHDFDDQPSRASKGPFSFRKSKNEALVEDTPMKGFVDDSDSLVPNQDLSPDIGKRTSEKEWWETQERGDQVVSADETGQVGPRVKCRCQVLRSVSQWSAGTSQVEESIHKAYCSLIETAEHFVYIENQFFISGLKGDEIIRNRVLEAMYRRIIRAHHEKKSFRIIIVIPLLPGFQGGIDDAGAASVRAIMHWQYRTICRGPNSILQNLYNLLGARMHDYISFYGLRSYGKLFEGGPVAASQVYVHSKVMIVDDCIVLIGSANINDRSLLGSRDSEIGVLIEDKELVDSFMGGKPWRAGKFASSLRHSLWSEHLGLSAVEINQIIDPVIDSTYKDIWMATAKTNTMIYQDVISCIPNDLIQSRSALRQCMTHWKEKLGHTTIDLGIAPVKLESYQDGNIRGTDPMERLKSVKGHLVSFPLDFMCKEDLRPGYKESEYYASPQVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKL3</accession>
    <name>A0AAF1AKL3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0103090</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKL3"/>
    <dbReference type="KEGG" id="dcr:108206718"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="156"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="104"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG83912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="230" mass="25801" checksum="2D456DE4A22BDB8D" modified="2024-05-29" version="1">MLTGDIPPNQTIYIKNLNEKVKKEELKRSLYALFSQYGRILDIVALKTPKLRGQAWLVFSEVTAASNAVRQMQNFPFYEKPMRIQYAKSKSDCVAKADGSFVPKDVKKKQEEKAERKKQESQPAGTNGVRAETNGGPMGSRQGKPSAQEAAAPPNNILFIQNLPHETTDIMLEVLFKQYPGFREVRMIDAKPGIAFVEFEDDNQSLVAMEALQGFKITPQNPMGISYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALR3</accession>
    <name>A0AAF1ALR3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0206567</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="16">Leaf</tissue>
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            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
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                <person name="Zheng Z."/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="37259" checksum="313B5D9C48ACCBA1" modified="2024-05-29" version="1">MTSTGTDDPRIIEEHWSHPDHPLELFQFIINEHDDDDEDDDDNTAILICDGCTQPITVTHSSYYSCTRCNFFLHSVCATKLPDELPTGVCSFHPQHSLALSKETRFYATVHCRVCGCLTNGFYYNCEACDMKIDICCAFMPSRIKYKSHKHHPFILRPSSGDVCSVSKSFIFGGMEYGCEICKNFQINVSSVFYPGTMKHKYEDHSITLRYPPFFYEGVFYCELCEERVNNQWWLYHCDESDHSFHSDCLLFWHRMKLGGTIRIDINDKMHTLAFVFKTNVRRNSPLHICGNCNYGYTSGLFFECDGCGYLICTRCVRKIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AR11</accession>
    <name>A0AAF1AR11_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0208427</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AR11"/>
    <dbReference type="KEGG" id="dcr:108205666"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF557">
        <property type="entry name" value="LIPASE_HYDROLASE FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041977668" evidence="5">
        <location>
            <begin position="20"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG89191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="367" mass="40650" checksum="364E4C701C98DC2C" modified="2024-05-29" version="1">MSFPLKITIINILILSSSAHGEMNTRPFGKIYAFGDSLTDTGNTQSGTGSNLPYGQTFFHHPTNRYSDGRLVIDFVAELLSLPYLPPYRNTTADKSSGVNFAVAGSTAIEHEFFVQNNITRDYTPESLETQLSWFTKFLESQGCIDIKATSQKCKAVFDEALIWVGEIGANDYAYIVGSSVSDRTIQELAIKRTTDFLEILLYKGAKYVVVQGLPPTGCLTLAMYLSREDDRDDIGCVGSVNKRSYSHNTIYQAKLQVFRKQFPDAIIVYADYWNAYHTVIKNAHNYGFNELYKVCCGSSGGQYNFDSSGPCGSPVSTTCANPSEYINWDGVHLTEGMNKVVSDLFLHGALTSPPFQYLLSIKKNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARD6</accession>
    <name>A0AAF1ARD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAB6-interacting golgin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0208743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARD6"/>
    <dbReference type="OMA" id="HACQKKE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007033">
        <property type="entry name" value="GORAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21470:SF10">
        <property type="entry name" value="RAB6-INTERACTING GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21470">
        <property type="entry name" value="RAB6-INTERACTING PROTEIN GORAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04949">
        <property type="entry name" value="Transcrip_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18662" checksum="25B058937FC5A361" modified="2024-05-29" version="1">MQTQQQAPGPVSQSFSFNGNLTKEEEEELSKSALSTFKAREAEIEKKKVEVRERVQAHLGRIQEETKRLADIRKELEGLADPLKKEVSVVRKKIDSVNKQLKPLGHTCQKKEKEYRNALEAFNDKNKEKVQLITRLMELVSESEKLRMKKLEELCKNIELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUM0</accession>
    <name>A0AAF1AUM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0414889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUM0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6460" checksum="2566F88F9A72F9CD" modified="2024-05-29" version="1">MYNTIIDGLCKSSNTSVALKLLRKMEEIGCKPDAITYNSIIDSLYKERRVDHALDLV</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94847.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="104" mass="11674" checksum="5085ED4F33362ADA" modified="2024-05-29" version="1">MVHTKFSSVSRSPSECASASKGKSRMSPKEVAMKKSKLKGKGKKTNFTIQDNVIAPPKGIQYVIPVKEHETAKLNTNNKYEQISDLLETLSVKQFDDFRASWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B027</accession>
    <name>A0AAF1B027_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="ORF">DCAR_0518834</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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            <begin position="21"/>
            <end position="769"/>
        </location>
    </feature>
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        </location>
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            <begin position="135"/>
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            <begin position="664"/>
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        </location>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B076</accession>
    <name>A0AAF1B076_DAUCS</name>
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        </recommendedName>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002473">
        <property type="entry name" value="Transducin family protein / WD-40 repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF40">
        <property type="entry name" value="WD AND TETRATRICOPEPTIDE REPEATS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="663"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG98895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="84011" checksum="4062D7C5C9AA2EC3" modified="2024-05-29" version="1">MDSFPFHDGNISQFIQHRSIETRSDINHGLQMHSSLVRRLSQEKELEGHQGCVNAVSWNSSGSLLISGSDDTRINIWSYSDHKLLQSVETGHSANIFCTKFVPETSDDIVVSGAGDAEVRVFHLSRLDGGGHEENTINPSAVFQCHTRRVKKLAVEAGNPNVVWSASEDGTLRQHDLREGASCPPASSAHQECRNILLDLRCGAKKSLSDTPKQNLALKSFEISSSRPHLLVVGGSDAFARLYDRRMLPPLSSSMKKLSPPPCVNYFCPMHLSDRAISSLHLTHVAFSPDGDEVLLNYSGEHVYLMDINPSSGSAMRYTSGDAMTLMSLNPILNGVDLKSSVSRVFPNGFPRKRNIVSRLDKCRKLVQIAEKSLKEGTNCYYAIEVCNEVLDGHGHEIGSVLMHECLCIRASLLLKRNWKNDAYMAIRDCQRARKIDSSSFRALHCISKALSLLRKHKEALDFAIAAETLAPSNSEIGRTMESIREELAAAESEKNKSSNIAPTNESRAQGTLSLSDILYRSEAHIDSSSDDPRSEREDSDYDEELDLDIETSISGDEGHDTEADVVHRSINLRFHGKDESAVGGADHANVSFGSPKSQSDEKTYQPEAVIDMKQRYCGHCNVGTDIKQASFLGQRGDYVASGSDDGRWFIWEKGTGRLMKVLLGDEAVVNCVQCHPFDCTIATSGIDNTIKIWTPSASVPSIVTGGAAGPETADVLDIMESNQRRLSRNREAMLPIELLERFRMHEFTEGTLHPFECTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4G1</accession>
    <name>A0AAF1B4G1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">DCAR_0522216</name>
        <name evidence="27" type="ORF">DCAR_0522219</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4G1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF89">
        <property type="entry name" value="OS06G0585982 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041894050" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="24"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="667"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="WOH02826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="WOH02829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="981" mass="107661" checksum="7B303E51C5AD5E81" modified="2024-05-29" version="1">MHMESYCMFNSLFLFMAFFIVSGEVNSSQILNDEASLLSFKSRVVYDPNNSLENWNSSKFQVCNWSGIGCNVNKTRVLSLDLSGLSLRGTISPAISKLSYLKILDLSKNFFEGHIPAEIGSLFHLKELSLSTNLLEGTIPRELGSLGDLVYLDLGSNRLSGEIPKSLLCNATSSLEYLDLSNNSISGEIRMNNQCQLRQLRFLLLWSNQLVGQVPPALSNSSKLEWLDLESNALHGNLPSEIVQKMPLLQFLYLSYNNFSSHNGNTNLSTFFSYLMNSSNLQELELAGNNLGGEIPSIIGDLPTCLVQLHLENNLIFGSIPPDISKLFNLTLLNLSGNLITGSIPRELCQMKRLERLYLSNNKLSGDIPSAFGDIPHLGLLDLSRNRLSGSIPDSFANLTQVRSLLLYENQLSGTIPPSLGNCINLEKLDLSHNSISGVIPKEVAGLSSLKLYLNLSANNLHGPIPGELGKMDMVLAIDISSNKLSGTIPPQIGSCIALEYLNLSSNALESSLPESLGKLPYLIGLDLSSNNLSGELPESLQASSTLKEMKFSFNNFSGVVLNRGAFSGLPFDSFMGNPHICGSIKGMHSCSRKRIYGAIILPAVISSVFVILILGILGYRLALKLNFKRKLATSTCPEMLEEEHEGNEHKHPKISYEQLIEATGGFSNACVIGSGQFGHVYKGIFEDDTKIAVKVINTKTAGELSGKCFRRECQILKRTRHRNLIKIITTCSRPDFKAIVLPLMPNGSLENYLYPSCSWNHRLDLVQLVNICSDVAEGLAYLHHYSPVKVVHCDLKPSNILLDHDMTALVSDFGIAKLVKESDQGSVCANDSALCISTDGLLCGSLGYMAPEYGMGMQASTEGDVYSYGVLLLESVTGKRPTDSIFQEGSNLHEWVKSQYPCKIEPVLEQALLRYAPHGSVVPSNRLWCDVLLELIELGLMCTQYSPLIRPTMDDVAHEMARLKQYLSGPSEKSGSTKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6W2</accession>
    <name>A0AAF1B6W2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0626277</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6W2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000008">
        <property type="entry name" value="U6 snRNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH06848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="281" mass="31399" checksum="AD7B867CF681C707" modified="2024-05-29" version="1">MEALMASYGGDTSSDSDSDETLPESTSSAPEPSKPPRLPPPPVSLLKSPKSLGILNQLETVQVSRVRSFAHVDGNYALHVYIPVRIPSGSKEKLARFIDKVSSLVPGLNVVDADVPLNILLKNNEKLEEIALGREFHISLGRTVAIRVHQIESVLSMLRQKPQLRRRYLINFDKWEVFVNDDHTRSFLSIEVLTGGLAEIARQIQAVNEVYKLHSLPEFYKDPRPHISIAWALGDISESLKRVVEAQQKSFLSVGELSRKPLFTCNFGEIECKIGSKTYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B9C6</accession>
    <name>A0AAF1B9C6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">assimilatory sulfite reductase (ferredoxin)</fullName>
            <ecNumber evidence="7">1.8.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0728200</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogen sulfide + 6 oxidized [2Fe-2S]-[ferredoxin] + 3 H2O = sulfite + 6 reduced [2Fe-2S]-[ferredoxin] + 7 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23132"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.8.7.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Interacts with ferredoxin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Plastid</location>
            <location evidence="5">Chloroplast stroma</location>
            <location evidence="5">Chloroplast nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.7.1" evidence="7"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B9C6"/>
    <dbReference type="KEGG" id="dcr:108196197"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042644">
        <property type="term" value="C:chloroplast nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016002">
        <property type="term" value="F:sulfite reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000008">
        <property type="entry name" value="Sulfite reductase [ferredoxin], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000014">
        <property type="entry name" value="Sulfite reductase [ferredoxin], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.480.10:FF:000001">
        <property type="entry name" value="Sulfite reductase [ferredoxin], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein,Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
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        <property type="entry name" value="SiR_ferredoxin-dep"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SIROHAEM"/>
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        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
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    </dbReference>
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        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="126"/>
            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="224"/>
            <end position="400"/>
        </location>
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            <begin position="418"/>
            <end position="477"/>
        </location>
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        <location>
            <begin position="497"/>
            <end position="634"/>
        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00003329"/>
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            <dbReference type="ARBA" id="ARBA00004595"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </source>
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            <dbReference type="EMBL" id="WOH09563.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="104" mass="12481" checksum="089BA3833578E64D" modified="2024-05-29" version="1">MDPVVYHPGPIDGSLLTLQKDHRSEAVWNRRERWRPETHTFHFSFGEATITLEDVWIYERFPTIAPHLRSQPQFTYPLALRYVICYSSHKRIQLLHMHYFSSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAF9</accession>
    <name>A0AAF1BAF9_DAUCS</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">DCAR_0730158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF45">
        <property type="entry name" value="PQ-LOOP REPEAT FAMILY PROTEIN _ TRANSMEMBRANE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH10688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="396" mass="43793" checksum="1BD94BB9EA38342D" modified="2024-05-29" version="1">MKNPLLSVVLKNSDVASSTSYCVKERRPCVGWVDKYFKDCLCDTRDKLSFASGMLSLISWGVAEIPQIITNFRSKSSRGVSLLFLLTWVLGDIFNLIGCLLEPVTLPTQYYTALLYTTSTVILVLQCLYYDHVYSWWKSKKVDANQPIEDVKKPLKPAKENDDDDSAIPTTDEGKINSNFYFTSARSLAGSATPPNRSYLWTARSGPSALTLDGDSSSDDEVDAAVMTNKKSKTQPKPIPRSAGYGAFLATSLNLPRSTKALLQASVAFSGRKLLQQGNADTEKFVVGQCLGWMMAAVYMGGRIPQIYLNIKRGSVEGLNPLMFIFALIANVTYVGSILIRSTEWDKIKANMPWLLDAVVCVGLDLFIILQYIYYRYLGKRTAGGGGQDDDYSESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAM7</accession>
    <name>A0AAF1BAM7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0832095</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAM7"/>
    <dbReference type="KEGG" id="dcr:108200032"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000355">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="150"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH12589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="425" mass="47983" checksum="DC726CF33A6D5334" modified="2024-05-29" version="1">MSGTSGDGNNAAAAAKKKGKAKVSRTSMILWHAHQNDASAVRKLLQEDRMLVLARDYDHRTPLHVASLHGWIEVAKCLLEFGADVNCQDRWKNTPLADAEGAKKHQMIELLKSYGGLSYGQNGSHFEPKPVAPPLPNKCDWEVEPTELDFSSAIIIGKGSFGEILKAFWRGTPVAVKRILPNLSDDRLVIQDFRHEVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHQYLKEKGALSPAVAVSFALDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSSAEHLKVGDFGLSKLIRVQHSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPPLAGYEPYEAAKYVAEGHRPTFKAKGFSPELKDLTDNCWKADMNKRPSFIEILKRLERIRDALPTDHHWSLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BBQ2</accession>
    <name>A0AAF1BBQ2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0831552</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BBQ2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="416"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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            <dbReference type="ARBA" id="ARBA00022982"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH12053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="58296" checksum="460A1D5966887607" modified="2024-05-29" version="1">MERKNIKTKPTLFSQVTPIVFFLPSNRPLEKQNLEPISLNIRIKSFNILSKNNKKKRESGNQWRWVAVISVFVVMLAFGLASKPFFSNPTSKSCQCSQGSHKYSGMVEDCCCEYETVDHLNEEGLYPLLQDLVKTPFFRYFKVKLWCDCPFWPEDGMCRLRDCSVCECAENEFPETFKKPFSHGLSPDNPVCQEGKPEAVVDRTIDAKAFKGWTVIDNPWTNDDETDNADMTYVNLQLNPERYTGYTGPSARRIWDAIYTENCPRYPSEEFCQEKKILYKLISGLHSSISIHIAADYLLEEATHVWGRNLTLMYDRVLRYPDRVENLYFTFLFVLRAVTKASDFLEQAEYDTGNSVEDLKSQSLVKGLLHNPKLQAACPLPFDEAKLWKGQSGPELKQQIQRQFRNISSLMDCVGCEKCRLWGKLQVLGLGTALKILFSVNGQDNMGHTLQLQHNEVIALMNLLNRLSESIKLVHEMGPAVEKAAGVPIPAFGDRTSIWQKLWNSVTGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD22</accession>
    <name>A0AAF1BD22_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0934781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH15244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein kinase regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="eIF-2-alpha kinase activator GCN1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.25.10.10:FF:000096">
        <property type="entry name" value="eIF-2-alpha kinase activator gcn1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GCN1, eIF2 alpha kinase activator homolog"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
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    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
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    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
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    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25786">
        <property type="entry name" value="HEAT_GCN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1326"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1501"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1621"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1643"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1739"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1912"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1967"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="2076"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="2393"/>
            <end position="2431"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="2518"/>
            <end position="2554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="798"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="812"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH15244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="2630" mass="288037" checksum="35774677F796ED76" modified="2024-05-29" version="1">MGGLELVSVADEFSIEFMEKRERDLGIDIGRRRFWTDVAHRIDNQEMTAEVVSLLVDLIFKTLYTYDERRSRNAVDSVIVKALRVDSFMKSFAATIVQVMEKQSKLQNHIGCYRLVKWSSLLLCKSLFPSISKNALCRVASAQASVIHIVKMQGSLREQRDCKKTIFSLFSESPQIYKTYIEELKDSRIPYKDSPELLSFILEYSHSKSTFFDQWKPVFLDMYVRVILNVKEKPSEGLSEAFRSLFSYLSHDEFKSIVVPSSVKMLKRSPELVLGSINVLLKSVNLDLSKYAIEILSVVLPQARHADEGRRLVALSIIKCLSEKSSNPDAIEAMFNAIKSVIGGSEGRLAFPYQRVGMVTALQELSSSLEGKYLSKISPEICKFLLSCYKDDGNEDVKLASLSALSSWAARSTDVIQPDLLSFMASGLKEKEALRRGHLRCILGVTKNVDAILRLSPLVGPLLLLVKTGFTKAVQRLDGIFALLILSKIAALDIKTEDTIAQERVWSLVSQNEPSIVPITMALKLSVEDCLACLELVEVLLVDHSQRVLETLSDKSLSQLLIFFLCHPCWDIRKVAQISLKKILGASPQICEALVVEYSNYLSVVEEKVILMKRSETETALDTQVPFVPSVEVLVKALLVMAPEVLAANPNTLLRIIVCSHHPCLVTTAKGNAVWKRLQKCLQRLSFDIIELIMADLGNICKGLLGPFGLMSSNHLEQEAAIHSLSTLMSITPTDTYTEFEKHLNNLPDRFEHDKLSENDIQIYLTPEGMLSTEQGVYIAELVTGNNQKQAKGRFRVYDNDNGSDNGASHSVKRDSSTAGKKDATKLTKKAEKGKTAKEEARELQLREEACVRDRVSSIQNTLSLMLKALGEMALSNPIFAHSQLPTLVKFVNPLLRSPIVGDAAFRTMIMLAKCSVAPLNNWALDLATALRLIATEESHRLCNIIPSDGDRVPKGGLFLGLFERIIMGLSVSCKTGPLPVDSFTFVFPIMERILLSSKKTGLHDDVLKILFLHMDPILPLPRLRMITVLYHVLGVIPANQASISPALNELCLGLQPDEVAPALAGIYAKDIHVRLACLNAVKCIPAVTSRSLPRSVEVATSIWIALHDVEKAFAEVAEDIWDRYGYEFGRDYSGLYKALSHVNYNVRVAAAEALAAVLDENPDTIQESLSTLFSLYIRDVGFGEDNIDAGWLGRQGTALALLSVADVLRTKDLPVVMTFLISRALADSNSDVRGRMLNAGIMIIDKHGKDNVSLLFPIFENYLNKKASNEETYDLVREGVVIFTGALAKHLGKDDPKVHAVVEKLLDVINTPSEAVQRAVSACLSPLMQSKEGDAPALVSKLLDQLMKSDKYGERRGAAFGLAGLVKGFRISCLKKYGIAAVLREGLADRNSAKCREGSLLAFECLCETLGKVFEPYVIQMLPLLLVSFSDQVVAVREAAECAARAMMSQLSAQGVKLVLPSLLKGLEDKAWRTKQSSVQLLGAMAYCAPQQLSQCLPKIVPKLTEVLTDTHPKVQSAAQTALQQVGSVIKNPEISALVPTLLLGLTDPNEHTRYSLDILLQTTFINSIDSPSLALLVPIIHRGLRERSSETKKKAAQIAGNMCSLVTEPKDMIPYINLLLPEIKKVLVDPIPEVRSVAARALGSLIRGMGEDKFPDLVPWLLDTLKSDGSNVERSGAAQGLSEVLAALGTDHFERILPDVIRNCSHQKASVRDGHLTLFKYLPRSLGVQFQNYLQQVLPAILDGLADENESVREAALSAGHVLVEHYATTSLPLLLPAVEDGIFSDNWRIRQSSVELLGDLLFKVAGTSGKALLEGGSDDEGSSTEAHGRAIIEVLGKDKRNEVLAALYMVRTDVSLSVRQAALHVWKTIVANTPKTLKEIMPVLMNTLISSLASASSERRQVAGRSLGELVRKLGERVLPLIIPILSKGLKDSSTSRRQGVCIGLSEVMASAGKSQLLSFMDDLIPTIRTALCDSTPEVRESAGLAFSTLYKNAGMQAIDEIVPTLLHALEDDKLSDTALDGLKQILSVRTTAVLPHILPKLVQLPLSAFNAHALGALAEVAGSSLNFHLGTVLPALLSAMGDNDQDIQKLARKAAETVVLVIDEEGVDSLIGELLKGVGDNKASIRKNSAYLIGYFFKNSKLYVVDEAPNMISNLIVMLSDSDQSTVIVSWEALARVIGSIPKEVLPSYIKLVRDAVSTSRDKERRKKKGGPVLVPGLCLPKALQPLLPIFLQGLISGSAELREQAALGLGELIEVTSEPALKEFVIPITGPLIRIIGDRFPWQVKSAILSTLCIMIRKGGIALKPFLPQLQTTFIKCLRDNTRTVRSSAALALGKLSALSARVDPLVGDLLSSLQSSESGVREAILTALKGVVKYAGKSVSSAVRVRVYSLLKDLIYSDDDQIRLSAASTLGIVSQYLEDDQFVELLDELTKSASSPDFCSRHGSVLTISSILRHEPSRLCGCTLFSEILDCLKVALNDEKFSVRETSTKALGRLLLHQTLSEPLVTNAHSEIVPSIVSAMQDDSSEVRRRALSVLKSVAKANPTVTMTYITVFGPSLADCLKDASTPVRLAAERCALHAFQLSKGTDNIQTSQKFITGLDARRISKFPEHSDDSEDSDNDLLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BE12</accession>
    <name>A0AAF1BE12_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0934078</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BE12"/>
    <dbReference type="KEGG" id="dcr:108200616"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF26">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041914839" description="Beta-hexosaminidase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="38"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="175"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH14558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="583" mass="65074" checksum="652727E87A305FD5" modified="2024-05-29" version="1">MKSHTFHFSPVVYILFILFLSNRNYIDIISAAGYPINVWPKPTTFEWTHPESVPLAPSFTITSPYHKYLTAAVNRYVRQILKEHHTPLITPSVDSPSTFPLKSLRISVTDISAPLTHGVSESYNLTVPGAGGVTAVMTAATPWAAMRGLETFSQLVWGNPGRVGSGLFISDAPLFGHRGVLLDTSRNFFSVDDIMRLIKGLSMNKMNVFHWHITDSHSFPIVVPSEPMLSEKGSYGGGMVYTPEDVKRVVEFGFEHGVRVLPEIDMPGHTGSWAEAYPEIVACAKMFWWPAGSEWADRLASEPGAGQLNPLNPKTYEVLKNVIRDVIAMFPEPFFHAGADEVIPGCWKVDKTIQTFLANNGTLSQVLEIFINTTYPYIVSQNRTVVYWEDVMLDDTIKVDPTFLPKETTILQTWNNGPNNTKRIVNAGYWTIVSSSDFYYLDCGHGGFVGNDSQYAQKPGTDQGKGGSWCAPFKTWQTIYNYDITYGLSKEQAKLVLGGEVALWTEQADATVLDSRVWPRASAMAEALWSGNVDESGMKRYAEATDRLIEWRQRMVNRGIGAEAIQPLWCIRNPTMCNTVQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BFS4</accession>
    <name>A0AAF1BFS4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrator complex subunit 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BFS4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="1">
        <location>
            <begin position="45"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH15262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="572" mass="65794" checksum="9C6049CA3DE769E0" modified="2024-05-29" version="1">MGSRLIVGAPHEADNLLETSLREAFELLESQLNPPFSLKCRNESEYFDINKAIIYGILCEPRMGNVHIKHLHGIVMIVDELYEKLVDSVRVQLIWITLEMIDVSAIGFQGLLVALLRQIVGGDFSDKNLWLCSELVRVFLAKWDCLLEEEPLVLTSALYAFLRLLAHHCRLIDFCVRLLREQFRLCMMIGRDLVRLLQELVHVPEFRSIWKDLMLSPSEFKVPEFRDISQLYRVRTSSRYFLLRITPVMESQLRFLLSDVKFGNHRRYQVWFAKKFLGIAEKETLLIDIVRFICCSHHPPNEIILSDIIPRWAVIGWLLKCCRKNYVEASVKLALFYDWLFFDEKVDNVMNIEPAFLLMVNSMSRYIEMTHTLLDFLLLVVNNYDTERREIIVHGVQSSFDVLVRRGVIQSLDVLISSDKLFPFLKERIVKLLSERKVTSLENFQAGSFRGSTSLPDLPAAVGMEPGTFQHEMSASASQNDVELGPPTNVTSCSPLAENDDIHEQSLESLVQNLGKHIRSSKQMGVQALDKILHLYVNLSSKAETTNFSISLDQLSSIIAKEFRYSGNQLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BIQ0</accession>
    <name>A0AAF1BIQ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BIQ0"/>
    <dbReference type="KEGG" id="dcr:108192629"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000108">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF862">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="175"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH15276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="526" mass="57383" checksum="C8C42C6D10ACD1C0" modified="2024-05-29" version="1">MGDPLAQKVFVFGSFSEEEARNLLNPSAENPVDKNGLLAGSLNNVPGLCFGSFDADLSISLPSYSNGSANFKPEGDEGKATQSVNVKVNGDVENSLHCSIIGNGSKGTTKEDELSESVLDDQVGSLEKLRLTSQDHNVVSLKHGSLNGTSHGNLQKHCEKAFDGPAKTVEDLQPRGLINSGNLCFLNATLQALLSCPPLVKLLLELKSHSISKVNYPTLAAFVDFVSVLEPNGITLKKKDIHVLETGRPFSPSMFEVVLKKFTPDVSSSISGRPRQEDAQEFLSFVMDQMHDELLKLEGKSSGNGGKLSLVSSSDDDEWETVGPKNKSAVTRTQNFVPSHLSEIFGGQLRSVVKARGNKDSATVQPFMLLHLDISHNAVRTIEDALHLYSAPETIDEYRASTTQKVGVVSAKKSISIQKLPKILILHLMRFGYGSYGSTKLHKPVRFSLELLLSRELLMTSATERRKYELVSTITHHGNEASKGHYTADARYPSGQWLRFDDASVTAIGASKVLHDQAYVLIYQQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BL25</accession>
    <name>A0AAF1BL25_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0936031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BL25"/>
    <dbReference type="KEGG" id="dcr:108201722"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF463">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH16476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="15095" checksum="0A16EEEC02171DDA" modified="2024-05-29" version="1">MGANQSAVYARNEANELFTPQKSGQVLTFHSSSKWRSHFQSSRQTNKLMVIHFTAAWCGPCRTMEPVIRDFAAKYVGVEFIQIDVDELEAVAREYAVQALPAFILIKKGKTVDKVAGAEKAALQNKIEKCMFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XEC9</accession>
    <name>A0A161XEC9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XEC9"/>
    <dbReference type="Gramene" id="KZM91083">
        <property type="protein sequence ID" value="KZM91083"/>
        <property type="gene ID" value="DCAR_021552"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224793"/>
    <dbReference type="OMA" id="NPPESCE"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000383">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="340" mass="37435" checksum="C97CF00EF4C8FABA" modified="2016-07-06" version="1">MAFDSVTNAKDFAKKKKANRSAKLKQCKLDARRHQWLSQVNNQERKDQVDVSGGIQVSPTHGRNERDRVVKISEMNSGNGEHDGSVQHYSDSDSSSHSRISHSCSVGGSHFSGTSFTGSGGSSRSSSSSGRSVSGIMSEEEDDDNNDDDDGCVDDWEAVADAMAASDAKQQQEQNKPFQESPSEHENVNKLNCPPEFPNSQLLEVDIPKPKAENRKAWRPDDAFRPQSLPTLLKQHSFPIKLERHSGSGGVTCAIGISTQSSCPICCEDLDFTDSSFLPCYCGFRLCLFCHKRILEEDGRCPGCRKKYEHDGITCEGNGNFAEGSSPYRFARSYSMITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XZK4</accession>
    <name>A0A161XZK4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_011011</name>
        <name evidence="5" type="ORF">DCAR_0312498</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XZK4"/>
    <dbReference type="STRING" id="79200.A0A161XZK4"/>
    <dbReference type="Gramene" id="KZN02257">
        <property type="protein sequence ID" value="KZN02257"/>
        <property type="gene ID" value="DCAR_011011"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212556"/>
    <dbReference type="OMA" id="METFHIN"/>
    <dbReference type="OrthoDB" id="1063785at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02043">
        <property type="entry name" value="serpinP_plants"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF340">
        <property type="entry name" value="SERPIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="22"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN02257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="403" mass="45449" checksum="B1CC624F96BB1ECC" modified="2016-07-06" version="1">MESSEQIKTSDLNSHFCIKLAVHIFLEEAEKGSNFVFSPVSLQILLRLIAIGSTGSTLDQILTCLGSKSTEDLNSLFSLVLDVTAGQNSYEKDLTTGPLVTMVNGSFIDQRFGLKSSYEGILRDVYKAEAKAVEFASKADQVIEEVNTWAEDVTSGLFKELLPPGSLGSDTALVFANALYFKGAWNQKFDLERSMHRDFYLLTGEIVQVPYMTTTKRERSIYREINGYKILKIPYQSGNDSGKFSMYFFLPLENNGLQHMVQNFNSTPGFFNQEFQLREENLGVRFWIPRFKFSFEFEASKTINELGLTLPFIAVKEFTELACSAHDEKLCVSKIFHKSFIDVNEEGTEAVASSGLARMSRRCRRVKPPGFIADHPFLFMIREETSRIVFFIGAVINPLLSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y4J0</accession>
    <name>A0A161Y4J0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205567</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group III family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y4J0"/>
    <dbReference type="Gramene" id="KZN04197">
        <property type="protein sequence ID" value="KZN04197"/>
        <property type="gene ID" value="DCAR_005034"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209169"/>
    <dbReference type="OMA" id="NQTYNCA"/>
    <dbReference type="OrthoDB" id="1888929at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010193">
        <property type="term" value="P:response to ozone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000009">
        <property type="entry name" value="WRKY transcription factor 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF36">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 41-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG86364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="336" mass="37610" checksum="0F9B3B60C1E42AB4" modified="2016-07-06" version="1">METSSEFEKSTIASELIQGMEVAKQLKFHLTTASSSPENLLTLLQKILSSYDTALLLLNWTESSAGQPQTLPAMLAQLPESPISTYESGDFKDHHRDVSKKRKALPTWKDHVRISSDNGGENSADGYSWRKYGQKDILGAAHPRSYYRCTYRKTRSCQATKQVQRSDNDSAIFEITYKGKHTCHKAGNSVPPAKPVKQEQNRSDHRPQPPNEALINFRANLRVKTDDCRIHEAADSFSFPSGMEYNSQLSSFSQLVNEDLMGTFSPSFISPDTTGSSYFSVSPYQMNSFGQLSDSDYTEIISANTSTTTSPILDLDFQIDEVNLDSNFPFDMPGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YB86</accession>
    <name>A0A161YB86_DAUCS</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0623196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YB86"/>
    <dbReference type="Gramene" id="KZM89789">
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        <property type="gene ID" value="DCAR_022848"/>
    </dbReference>
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    <dbReference type="OMA" id="LTYVRCR"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="At5g54830-like"/>
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        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
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        <property type="entry name" value="DM13_domain"/>
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        <property type="entry name" value="DOH_DOMON"/>
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        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47281">
        <property type="entry name" value="OS09G0557700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47281:SF1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DoH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043467006" evidence="9">
        <location>
            <begin position="23"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="741"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="813"/>
            <end position="834"/>
        </location>
    </feature>
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        <location>
            <begin position="860"/>
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        </location>
    </feature>
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        <location>
            <begin position="872"/>
            <end position="891"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="891" mass="99359" checksum="62835A2C5678AAF5" modified="2016-07-06" version="1">MTRSSLFLLLSCWVFLNSLVKSDPGSSCSKSNTSLIDYETQFSMIQHQVRGVFTILDDCSFRVSKFDMLKGSDVHWWGSTGVDFENMTRGFLLSDDKLDKTFSNDSFVVNLRKNVSWDLVKVVSIWDVPTASDFGHVVLGNLGDGLEAPAPTPSNGSRGGVQNVPTMFQNCKQLSSDFRVRWTLAAEDNVIDIGLEAGIAIQNYMAFGWAKPGSSNPMLGGDVAVTGFTDDGLPFAEDYYITRYSQCMMTADGFQGVCPDTFYEPAEEVSFLNNTKLVYAHRKDGVSFIRFQRMLNSDDKKYDVQVNSTENMTVIWALGSMKPPDMLRPYYLPQKHGESYGYLKINVSEHINDCLGPLDAEDKEDQDLLIADGKEALVISTGPALHYPNPPNPSKVIYINRKEAPLLRVERGVPIKFSIQAGHNVAFYVTSDPLGGNATLRNLTETIYFGGPKAEGVQSSPTELIWAPNRNTPDQVYYQNLFTQKMGWKVQVVDGGLPDMYNNSVVLDDQQVTLFWTLSETSISVAARGEKKSGYLAIGFGSEMVNSYAYVGWMDSNGTGGVSTYWIDGRDAANIHPTNENLTYVRCKSENGVITLEFTRPLKPSCDRDERPECNNIIDPTTPLKVIWAMGAQWSGKHLSERNMHSITSSRPVRVLLVSGSAEAEEDLRPVLAVHGFMMFLAWGILLPGGILSARYLKHLKGDGWFKIHVYMQYSGLAIIFLGFLFAVAELRGLYLDSLHVKFGMGAILLACVQPVNAYLRPKKSASGEVVSSNRILWEYIHVIVGRCSIVVGMAALFSGMKHLGERYDGENVQGVTWALIIWFLIGVLITLYLEYREKQRRRESFLARSNWVLGNGEDEDHDLLSPSGMSVDKDLHPSDRMEVQLEPLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YJD7</accession>
    <name>A0A161YJD7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0519021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YJD7"/>
    <dbReference type="Gramene" id="KZM93399">
        <property type="protein sequence ID" value="KZM93399"/>
        <property type="gene ID" value="DCAR_016644"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223565"/>
    <dbReference type="OMA" id="YSCMVYL"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017088">
        <property type="entry name" value="Wax_synthase_Magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037006">
        <property type="entry name" value="Wax_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG99667.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="346" mass="38907" checksum="01CE905E61BE97D3" modified="2016-07-06" version="1">MEGEMNKLGYVCSSVIASLCYCYFVSSKISPGLSRFLSVLPIFCLFIILPYYLSSAFNTALITFFTTWLSNFKLLLFCFGHGPLSSPNCSLTFFISIASLPIRIRQNYDTHKRVLPLNFSLKVLVFAVLLKVSCCYGGALHHKMIQCVYCCLCFLLIEILIGLSNTLSGALVGGEFEFEPPSNEPYLSTSLQDFWGKRWNLTVTTSLRDTVYKPVRKASEVVLGKNLAPVPAVMAAFLVSGLMHELLFYNVTRSYPTWEMTGFFVLHGICVVVEVGLKNWARGRGWRLPWPVSTGLTVGFVLATSLWLFFPPLIRSGADTIVLEEMAFFVQLMWTHLTNLFSSIYK</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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</entry>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="6">Leaf</tissue>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
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        <property type="protein sequence ID" value="WOG97875.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SufE-like protein, chloroplastic"/>
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    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF3">
        <property type="entry name" value="SUFE-LIKE PROTEIN 1, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fe-S metabolism associated" evidence="5">
        <location>
            <begin position="87"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM98860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG97875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="356" mass="39015" checksum="F2A9289437DFAB49" modified="2016-07-06" version="1">MAVSTSFRVFSTRFPQPILHPTKTPVFQTLFLSTIPPQTPTKISFFRSISIQKVPTTPHASSSSTPSASVNLQPISDLPPKLQEIVKLFQSVQEPKAKYEQLLFYGRNLKPLDARFKISDNKVQGCVSQVWVRAYFDDDKNVVFEADSDSVLTKGLAALLVQGLSGEPVEEIVRVSPDFAVLLGLQQSLTPSRNNGFLNMLKLMQKKALNLFIEAEKSGSNEMVVESLSLGSSTDKDGDLFVGDGVEVDGGDSGSSSDNVIALGSRGERIREKLERELEPVELKVEDISYQHAGHAGVRGSDGETHFNVRVVSKEFEGKSLVKRHRLIYGLLQDELQSGLHALSIEAKTPTEVGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ADD6</accession>
    <name>A0A162ADD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADPH-protochlorophyllide oxidoreductase</fullName>
            <ecNumber evidence="7">1.3.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_013086</name>
        <name evidence="9" type="ORF">DCAR_0418007</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>chlorophyllide a + NADP(+) = protochlorophyllide a + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83348"/>
            <dbReference type="ChEBI" id="CHEBI:83350"/>
            <dbReference type="EC" id="1.3.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.33" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ADD6"/>
    <dbReference type="SMR" id="A0A162ADD6"/>
    <dbReference type="STRING" id="79200.A0A162ADD6"/>
    <dbReference type="Gramene" id="KZM99552">
        <property type="protein sequence ID" value="KZM99552"/>
        <property type="gene ID" value="DCAR_013086"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219384"/>
    <dbReference type="OMA" id="FRFKREH"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="UniPathway" id="UPA00668"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016630">
        <property type="term" value="F:protochlorophyllide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09810">
        <property type="entry name" value="LPOR_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005979">
        <property type="entry name" value="Prochl_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01289">
        <property type="entry name" value="LPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419:SF19">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE A, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM99552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG98663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="398" mass="43365" checksum="C817D57FA7CF27D5" modified="2016-07-06" version="1">MALQAASFLPSSFSINKEGKANVSLKETSLFGVTFSDSLRTDFSSLRTRRGCRQISQTGAIRSQAVATTPSVNRATGEGKKTLRKGSVIITGASSGLGLATAKALAETGKWHVIMACRDFLKAERAAKSAGMPKENYTIMHLDLASLDSVRQFVETFRRSERPLDVLVCNAAVYFPTAKEPTYTADGFELSVGTNHLGHFLLSRLLLDDLNKSDYPSKRLIIVGSITGNTNTLAGNVPPKANLGDLRGLAGGLNGMNSSAMIDGAEFDGAKAYKDSKVCNMLTMQEFHRRYHEETGITFASLYPGCIATTGLFREHIPLFRTLFPPFQKYITKGYVSEAESGKRLAQVVSEPSLTKSGVYWSWNKDSASFENQLSEEASDVEKARKVWEVSEKLVGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AR79</accession>
    <name>A0A162AR79_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0206218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AR79"/>
    <dbReference type="Gramene" id="KZN04683">
        <property type="protein sequence ID" value="KZN04683"/>
        <property type="gene ID" value="DCAR_005520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043926724" evidence="2">
        <location>
            <begin position="20"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="147" mass="16222" checksum="3A8BA54FF1B38712" modified="2016-07-06" version="1">MSKLVVALITATLVAVVSAQGGDLQSCLFNCRLRVISCATGCRIGAGPFPFNIRLCLGQCSFQNFQCLQSCMNPRPPPQRPHHPHFHRPPKGPRRPHFHRPPKSPLEPHPIVPPPQTLKPQSLPSASAYFLPLPPPPSQLHHFEKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AT61</accession>
    <name>A0A162AT61_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0207205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="gene ID" value="DCAR_006390"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
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    </dbReference>
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        <property type="entry name" value="OS02G0822800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Acyl-CoA-binding" evidence="3">
        <location>
            <begin position="381"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG87972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="498" mass="54469" checksum="0E3BD4C4F9BEA9DD" modified="2016-07-06" version="1">MGTEIQNWRLELDYDQWIALPVSGPRPSARYKHAAAVVGEKLYIVGGSRNGRYLSDFQVFDLNNLTWSSIKLNVDPNSKTVENSTPEIAFPAISSHNMIKWENQLIIVGGQTKNISDRVTVRFINLESHLCGVVETTGNIPIARSGQSVSLFGSKLIMFGGEDKSRRLLNDVHVLDLETMSWSDLETTQTTPAPRFDHTAAVHAGRYLLIFGGCSHSVFFGDLHVLDLETMEWSQPEVQGEIVAPRAGHAGVTVDDNWYIVGGGDNRSGAQETLALNMSKLVISILTSVKGRDPLASEGLSVSSSIIHGEKFLVAFGGYNGKYNNEVYVMRPKPNDSAHAKIFKSPAAAAAAASVTAAYALTTSEVAITDTRGPNIEVDLSVEISTVKENKKLLESSLSDIQAENSALQAKLDDTNSTHVELSKELQSVQGQLVAERSRCAKLEAQIAELQKMLVSLQSIEEEVQEIRRQKAEFDRDIEVASADQRQSSGGVWRWVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B2F9</accession>
    <name>A0A162B2F9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_001799</name>
        <name evidence="6" type="ORF">DCAR_0101800</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B2F9"/>
    <dbReference type="STRING" id="79200.A0A162B2F9"/>
    <dbReference type="Gramene" id="KZN09143">
        <property type="protein sequence ID" value="KZN09143"/>
        <property type="gene ID" value="DCAR_001799"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200833"/>
    <dbReference type="OMA" id="HHVDDSQ"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000146">
        <property type="entry name" value="protein RAE1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="64"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN09143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="345" mass="38427" checksum="FA9B79706E33D54B" modified="2016-07-06" version="1">MSFGVADTNPNKSFEVTHPPTDSVSSLSFSPKANYLVATSWDNQVRCWEVMRNGNNLGTVPKAQISHEQPALCSTWKDDGTTVFTGGCDKQVKMWQLSSGGQPVTVGMHDAAVKDLAWIPEINLLVTGSWDKTLRYWDLRQPNPAHVQALPERCYALTVRHPLMVVATADRNLIVYNLQNPQTEFKRISSPLKYQTRCVAAFPDQQGFLVGSIEGRVGVHHLDDSQQTKNFTFKCHRDGNDIYSVNALNFHPVHHTFATAGSDGTFNFWDKDSKQRLKAMSKCSNPIPCSAFNNDGSIYAYAVCYDWSKGAENHNPSTAKTCIYLHATQEAEVKAKPRVTTGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UT68</accession>
    <name>A0A164UT68_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase I subunit rpa49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_026403</name>
        <name evidence="8" type="ORF">DCAR_0730312</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A164UT68"/>
    <dbReference type="Gramene" id="KZM89328">
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        <property type="gene ID" value="DCAR_026403"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196796"/>
    <dbReference type="OMA" id="DVYPFDE"/>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM89328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH10838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="51485" checksum="ECA7B77FDA0C214B" modified="2016-07-06" version="1">MGSKHKDKKKHKSHENPQTQLDLDDFEQDNNANNDDNLVETEPKKPKNKNKKKKETLDVKIQTIHAKSDKISPLVGYFPTTFDPFKGSGSSSVDENSEVTVYQNVQRSSRYQVVVKPNGSDVNFVGTNYSGEAAAPQICTYALGVLDKETQTLRIVPITSNKIFRLEPNFGESDTTQDEAQQILKDEVSREERTQNLRRLDTMFSTKKTITKNKKIDSLRQKQDPESKEDLEKSLKDVKVNTEAIDAGGTNVRNIPFHDMSASTPEKAYPLEKIIEKAEWDYLGDLLKITEGAGVTPDTYPIFVCNRVNKLKDIKDEYQRSTVASVLQYITHLIKYKDKHSMDHYRSVKHHKFPSILEGKFRTMFNEPGAKRLAAEKHKFLISHVLVLTLFVDGYKSDTSDIAKDLKISVVDLRKHWAELGCKLVREKSTTFATLPLPLTFPVIRQKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VN33</accession>
    <name>A0A164VN33_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0624126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VN33"/>
    <dbReference type="Gramene" id="KZM90595">
        <property type="protein sequence ID" value="KZM90595"/>
        <property type="gene ID" value="DCAR_022040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF135">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0621600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH04714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="385" mass="44235" checksum="C730702F8965B368" modified="2016-07-06" version="1">MESSGLVADKFCWKMSHMDTSSDELSLPHTFCAKYAHRLHEDMHLKLRNGYIIPVKFDKSKVTLGDGGWRFVWFNLERTTTFDEIAPPDAFLICCAFSFSEPMTYVISNGKKFSCSYCHTSRKFKGLGFLSDSLGLTAMPALHMLLFTYEVDSVVSITAFDEQLCEIVFPGHPLSSEGDSHDSSVLKPVSLHFKITVEKRHMSTDCDLIVKMERTGFVAPKFIKVLSRKEICLNVMMLPSDFKLKYGDKLPPEFELKLRTGYNLPIDYDKNTGLMKGMSIFYRHFAFKGGEVLVFEYYGRFDLNLYILGADCCEIEYPMILHHFQTGNALLPKVRDHGWKFVHFTNNLQRVVNDIVCYDVNKISCSVYHNIFYLSHYLSVTVLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VVS1</accession>
    <name>A0A164VVS1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_021701</name>
        <name evidence="14" type="ORF">DCAR_0624511</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VVS1"/>
    <dbReference type="STRING" id="79200.A0A164VVS1"/>
    <dbReference type="Gramene" id="KZM90934">
        <property type="protein sequence ID" value="KZM90934"/>
        <property type="gene ID" value="DCAR_021701"/>
    </dbReference>
    <dbReference type="OMA" id="KHACRDD"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000029">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000358">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="80"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM90934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH05098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="373" mass="42812" checksum="638354D22E4557D9" modified="2016-07-06" version="1">MLDLIWNEQPGQEVIYQWVEWVQSSSLSYLGFDEEIILGPYEIIHTGDKRAISESVSPDVDIPSLKSYNDEQCHENFVKNFHECHICYSEYPGGDFIKLSRQHFFCWKCMETYVGITAREGTKTKLSCPEEKCEGVLPSGILRRLLGDEEFQRWETLVMQKTLASMSDVVYCPRCETTCCADEDNDAQCSKCFFSFCSLCSEKRHIGTSCMTPEMKLLVLQRREKEMINEILSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGNYFCYRCNQKIDGYEHFKGECELFPQEMIQTWERANARMVEGQILADLFGGRGHPCPLCAQVNAKVGNNNHIFCWACGNHYCYLCKKIVKRGSNHYGPKGCKQHTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WUU1</accession>
    <name>A0A164WUU1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0626057</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Microsome membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WUU1"/>
    <dbReference type="Gramene" id="KZM92283">
        <property type="protein sequence ID" value="KZM92283"/>
        <property type="gene ID" value="DCAR_020352"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224635"/>
    <dbReference type="OMA" id="MAGEKRM"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF144">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0492">Microsome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH06629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="15128" checksum="EB769F4396C6C08B" modified="2016-07-06" version="1">MGSDPKIHVFEEVAKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATDDFEDVGHSDNAREMMEKYYIGEIDAASVPQKRTFSAPKQSNYNPDKSSEFIIKILQFLVPILILGLAFVVRQYTKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z2I7</accession>
    <name>A0A164Z2I7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Poly [ADP-ribose] polymerase</fullName>
            <ecNumber evidence="18">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0521139</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="18"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z2I7"/>
    <dbReference type="Gramene" id="KZM95225">
        <property type="protein sequence ID" value="KZM95225"/>
        <property type="gene ID" value="DCAR_018467"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223996"/>
    <dbReference type="OMA" id="MNFKYKY"/>
    <dbReference type="OrthoDB" id="2017365at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000004">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000051">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008288">
        <property type="entry name" value="PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049296">
        <property type="entry name" value="PARP1-like_PADR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF80">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21728">
        <property type="entry name" value="PADR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08063">
        <property type="entry name" value="Zn_ribbon_PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000489">
        <property type="entry name" value="NAD_ADPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01335">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52007">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="13 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="189"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH01754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="995" mass="112155" checksum="79944A9FA78CC1A2" modified="2016-07-06" version="1">MASPPKPWKAEYAKSGRSSCRTCKSPIDKEAFRLGKMVQSSQFDGFMPMWNHASCILKKANQIKSQDDVDGLDMLRWEDQEKIRKYADGSEAPKSGGSRSSRAAASAATASGIEVSQSSRATCRQCNQKIMKGDVRISAKPEGQGAKGVTWHHANCYMESSPTIEVNKLSGWDDLSASDQTAVIAMIEKSTSKGPINDPVDDTKPMQQSTSKGNAKRKSASTSEQKLKVAKAEGDVLPNTPKKNADALGIDSPRASTVDRELETQSKELWSLKDDLRKHVTTSELREMLEVNEQNSKGSELDLRDRCADGMLFGKLENCPLCSGYLYYSGGMYKCSGFLSAWSKCSYSTTESVRVQGKWKIPEGSENEYLVKWFKSQKVKKPVRVLPPPTPSKPSGTPSTKDGRLEDLKVAFNGLSGPAMEEWRSKLGEVGVQIHEKVKKETNCLVVSEMVNGQNAEVKKARRMKLPIVRENYLVDCLKRQKKLPFDLYKIEALGETSGSDVVTVKVKGRGAVHEASGLQDTGHILEVGKSIYNTTLNLSDLSTGVNSYYILQIIEEDKGSNCYVFRKWGRVGNEKIGGNKLDERDKEDAIEEFERLFLEKTGNTWRAWERKVDFQKRPGKFFPLEIDYGVNKEMVQRKNFKDSDSKLARPLLELMKMLFNVETYRAAMMEFEINMSEMPLGKLSKRNIQQGFEALTEIQNLLSSSAYNPTVRDSMIVDASNRFFTMIPSVHPHVIRDEDDFKSKVKMLEALQDIEIASRLVGFDTDDSVSVDEKYKMLHCEMNPLPHNSEDYRLVEKYLQTTHAPTHSDWSLELEEVFSLEREGEFDKFAPYRDKLKNKMLLWHGSRLTNFVGILSQGLRIAPPEAPATGYMFGKGVYFADLVSKSAQYCYTDKKNPVGLMILSEVALGEVYELKGAKYMDKPPRGKHSTKGLGRQVPQESEYVKWRDDVIVPCGKPVPSNVKATELNYNEYIVYNTAQVKMQFLLKVRFHHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A678</accession>
    <name>A0A165A678_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0415069</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Regulates the production of reactive oxygen species (ROS) by NADPH oxidase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A678"/>
    <dbReference type="Gramene" id="KZM96983">
        <property type="protein sequence ID" value="KZM96983"/>
        <property type="gene ID" value="DCAR_015655"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217643"/>
    <dbReference type="OMA" id="MRHPWIC"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000178">
        <property type="entry name" value="Calcium-dependent protein kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG95742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="566" mass="63304" checksum="5D6DFE841F14F973" modified="2016-07-06" version="1">MGNTCRGSFGVKQYQEGGLIPPQEHSSSKGSSNNNSNNSTTTITPQEFVKENNHSPIPDNNHKDHILALVSPKKDNTMNRTGNSQSHYVLGHITPNIRDLYTLGRKLGQGQFGTTYLCTENTTGTEYACKSISKRKLISREDVEDVRREIQIMHHLAGHSNIVTIKGAYEDHLYVHIVMELCNGGELFDRIIQRGHYSERKAAELTKIIVGVVESCHSLGVMHRDLKPENFLLVNKDDDFSLKAIDFGLSVFFKPGQIFSDVVGSPYYVAPEVLLKHYGPEADVWTAGVILYILLSGVPPFWAETQQGIFDAVLKGTIDFDSDPWPVISDSAKDLITKMLCSRPSDRLTAHEVLCHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFKTMDTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVAATLHLNKLEREEHLVAAFKYFDKDSSGYITVDELQQAWAEHNMTDVFLEDIIKEVDQDNDGRIDYGEFVAMMQKDNAGIGRRTMRNSLNISMRDVDVRGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WEN6</accession>
    <name>A0A165WEN6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_015244</name>
        <name evidence="11" type="ORF">DCAR_0415548</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WEN6"/>
    <dbReference type="STRING" id="79200.A0A165WEN6"/>
    <dbReference type="Gramene" id="KZM97394">
        <property type="protein sequence ID" value="KZM97394"/>
        <property type="gene ID" value="DCAR_015244"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215661"/>
    <dbReference type="OMA" id="CYKNKVF"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.900:FF:000001">
        <property type="entry name" value="SBDS, ribosome maturation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1250.10:FF:000001">
        <property type="entry name" value="SBDS, ribosome maturation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="U1-type" evidence="9">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM97394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="366" mass="41733" checksum="35F547DB21AFA2A5" modified="2016-07-06" version="1">MSKTIVQPVGQKRLTNVAVVRLKKHGCRFEIACYKNKVLSWRSGVEKDLDEVLQSHTVYSNVSKGVLAKSRDLKKAFGQDEYDDDEKIDHEKICLEILEKGELQVAGKERESQLSSQFRDIATIVMQKTINPETHRPYTISMIERLMHEIHFAVDPHNSSKKQALEVIRELQKHFPIKRSPMRLRLTVPGQAFSSVSEKLNEWKSSIVSKEESGNQLSVICEMEPGFFRDCDAMVRNLQGRLEILAISVHAEGDTNVDHYDDHEEPSSSLPKASTGADFQLSEKLNKQTISEKKPDAVGDVKQQSKCSTCNAYVGDSKQYREHFKSEWHKHNLKRKTKQLPPVSAEECLGDMEITDSRADLKDYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WJW7</accession>
    <name>A0A165WJW7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0415614</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WJW7"/>
    <dbReference type="Gramene" id="KZM97449">
        <property type="protein sequence ID" value="KZM97449"/>
        <property type="gene ID" value="DCAR_015189"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219485"/>
    <dbReference type="OMA" id="NMFLPDR"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methionine--tRNA ligase, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="methionine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Met_tRNA_synth_type2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
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        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
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        <property type="entry name" value="MetRS_core"/>
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    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008900">
        <property type="entry name" value="PRK12267.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
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        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
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        <property type="entry name" value="Nucleotidylyl transferase"/>
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    </dbReference>
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    <keyword evidence="6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
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    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG96280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="613" mass="68046" checksum="BE0AA85F351B9DD3" modified="2016-07-06" version="1">MAGRAHFSIQRNGTLLLSSSSFFHNSINFGLTHLKNPNFFRPKGLSYSSRGALYCSCSASNGLEKQLSSDPFVLTTPLYYVNAPPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAASGSSPSEHCDVVSQAYKELWKDLDIAYDKFIRTTESKHEAIVKEFYAKVLASGDIYRADYEGLYCVNCEEYKDEKELLDNNCCPMHLKPCVSRKEDNYFFALSKYQKRLEDTLQQNPGFVQPSYRLHEVQGWIKGGLRDFSISRAAVDWGITVPNDTDQTIYVWFDALLGYISALSEDKEHSDLQSAISSGWPASLHLIGKDILRFHAVYWPAMLMSAGLDLPKMVFGHGFLTKDGMKMGKSLGNTIDPKSLVDRFDSDAVRYFFLREVELGNDGDYSEERFINIVNANLANTIGNLLNRTLGLLKKNCNSTLPVDSAVAAEGNTFKDSAEDLVAKARTEYENLAISSACEAVLEIGNAGNLYINERAPWSLLKQGGDAFEAATKDLVIVLEAMRIIAVALSPITPSLCLRVYEQLGYTEEQFNAATWNETKWGGLKSGHVMAQPRPVFARIEVQKEEEDVAQVVKKVSKKKAKIPQVQTVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YRI4</accession>
    <name>A0A165YRI4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_013480</name>
        <name evidence="6" type="ORF">DCAR_0417554</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YRI4"/>
    <dbReference type="SMR" id="A0A165YRI4"/>
    <dbReference type="STRING" id="79200.A0A165YRI4"/>
    <dbReference type="Gramene" id="KZM99158">
        <property type="protein sequence ID" value="KZM99158"/>
        <property type="gene ID" value="DCAR_013480"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218111"/>
    <dbReference type="OMA" id="QKCCGEL"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MSP" evidence="4">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="392"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="425"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM99158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG98213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="541" mass="59073" checksum="C6534A81FF43E218" modified="2016-07-06" version="1">MDRLISLEPSNLVAIRIRKGQRCYGELTLRNVMHTMPVAFRLQPMDRNHYTVRPQSGIISPLTTVTVQITYESHQHSVLPESFPYCDDSFLLHSVVVPGATVKNPASTYDTIPNEWFTTRKKQVFIDSGIQVMFVGSPVLTQLVNNGAMGEIRDVLEKSDSAWKAVESVDSDGQTLLHLAISKNRPDLVQLLLEFEPNVEVSSGSGSTPLEAAVALGETLIVELLLAHHANVERSEASSWGPIHYAAGGGYLEILKLLLLKNADVNALTKDGNTALHIAVEERRRDCARQLLTNGARTDICNSGDGDTPLHIAAALADDHMIKLLLQKGANKDIRNKVGKTAYDVAAEHGHSRLYDALRLGESLSIAARKGEVRTMQRLLENGALINGRDQHGWTVLHRASFKGHLDAVRILIEKGIDINAKDVDGYTALHCAVESGHVEVIELLVKKGAEVESKTNKGVTAIQIAESLNYSGITRILVYGGATKDGSSVKKYGLPPTSLVSFGKGKGVKEIMDVAVKKKKATHPRNVRGSYDRTRTMAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C0D6</accession>
    <name>A0A166C0D6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0313413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN03120">
        <property type="protein sequence ID" value="KZN03120"/>
        <property type="gene ID" value="DCAR_011876"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33430:SF1">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG94120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="187" mass="20185" checksum="A4208B2C56810ABE" modified="2016-07-06" version="1">MANNDKFDVKEVHLKLLDDLVNVNSLLTIAVFVGLSMATPGIKSLDSRQECHAGPREAKMLILYEVIAFSCFLLSSIVAKVLKLHLYLDGADKYAFTTPDFDLKEFMLAISACGSVAGIISLSLSIVNIIQIRIGLLSCGCREATAAVLCLGILVGFALVIYVVSMVIAIYASFKSDHAKVLEKGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C7J6</accession>
    <name>A0A166C7J6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0313769</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3 7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione S-transferase U10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tau class glutathione S-transferase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF676">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
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    </dbReference>
    <dbReference type="Pfam" id="PF02798">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
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    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG94473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="220" mass="24978" checksum="B89BA39B6946E936" modified="2016-07-06" version="1">MATQEVKVFGVWESPYSKRVEIALKMKGVEYEYVEEDLSTKSSELLKYNPVHKKVPVFLHNGKPIVESLVILEYIDETWKSGTPILPEDPHERATSRFWANFVDDKLLPAAVKYLKSKGGEKAVNEEISELLSVLENELKGKKFFGGDSIGLIDIASNIIALWLDVIQEVVGVEVFTKEKHPKLFKWSEEYLNCSIIKETLPPRADLLAFWQSRVQAPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CMS3</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CMS3"/>
    <dbReference type="Gramene" id="KZN04109">
        <property type="protein sequence ID" value="KZN04109"/>
        <property type="gene ID" value="DCAR_004946"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208442"/>
    <dbReference type="OMA" id="VQTEEHI"/>
    <dbReference type="OrthoDB" id="1911637at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049306">
        <property type="entry name" value="GLV1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053313">
        <property type="entry name" value="RGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21529">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043590357" evidence="2">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="161" mass="17993" checksum="24F1FB6AB77F15D0" modified="2016-07-06" version="1">MSKLVFVLLVFLVSLHACNSRGLSLSADRKTDSIFHTIGKDVHSRKLRSLFINSEPTVVQTEEHISTSTTGTDEFEDEVMGKDSAADSLKKEGKKGGKVALSYLEETAQIEKWRQARSTLESSPNEVKETAKSEDDETMDDVVSMDYAKPHRKPPIHNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166E2P9</accession>
    <name>A0A166E2P9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_006845</name>
        <name evidence="12" type="ORF">DCAR_0207710</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_006845"/>
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    <dbReference type="KEGG" id="dcr:108205942"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Myb_DNA-binding"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="3"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="89"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="89"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="96"/>
        </location>
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        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG88475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="328" mass="36027" checksum="535EEA1FB2DA0B24" modified="2016-07-06" version="1">MTRRCSHCSNNGHNSRTCPNRGVKLFGVRLTDGSSSIRKSVSMGNLSHYAGSSLKNGHDSPGDNLDHGGDVADGYGSEDFVPGSSSSGRERKKGVPWTEEEHRMFLLGLQKLGKGDWRGISRNYVITRTPTQVASHAQKYFIRQCSMSRRKRRSSLFDMITDEPVETPMVSHDFFSVNTQEDETQIDPLPAPAVEQECEPMESTKSNDVEPSSFQPEISQYCYPVYYPAYISSVPTSHPYWSGCSEEPRKIESHVVLKPTAVHSKSPINVNELVGMSKLSLGASIGRDETSTLSVKLLEGPSRQSAFQAKPTPANSGMISGNNAIHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ELX1</accession>
    <name>A0A166ELX1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_007579</name>
        <name evidence="13" type="ORF">DCAR_0208560</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the early nodulin-like (ENODL) family.</text>
    </comment>
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        <property type="protein sequence ID" value="KZN06742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="WOG89322.1"/>
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        <property type="gene ID" value="DCAR_007579"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Early nodulin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041846">
        <property type="entry name" value="ENL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF234">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="11">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN06742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG89322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="21911" checksum="AFB4F53C037C95C1" modified="2016-07-06" version="1">MASSPPGCHFGFDIYCLFVTILVIVAARAIITPASAVEEFKVGDVDGWRQPDANHTEIYSLWTAMKRFHVGDSLRFEYKNDSVLVVNKWAYYHCNITNASSVFTDGNTTINLDNAGPLYFISGDVEHCENGQRLAVVVLPLHPHIHKSPPPLVAVDEDSPTASPVSTSRADTDHVYVPKTLPYSVLVTATLVSLLWHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FSB4</accession>
    <name>A0A166FSB4_DAUCS</name>
    <protein>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="4">Leaf</tissue>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="DCAR_000786"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG82751.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26221" checksum="5C2C5595F82B6703" modified="2016-07-06" version="1">MFLSLNELTKIEEKVVLIYNKKSSCIGHGALIDSNGLILTASHLFDESTVIGYRVNVRVNGGRATQMYVARVVKLEWKWDIALLQIEVPNKLKYAKFAKKVRVGQDLHMVVSSDGDILSYTSGTVAFERRPFGDIVHSKNIDYFDPETIFVQAHNLHGQPGCSGAPIFSSKGRIVGMYSSAYLKSDLLVHVEHLKTLSYSFIQEGETSEAGGKKKRKQKKKRGETSEAGGKKKRK</sequence>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_002250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG83156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="49" mass="5546" checksum="5376D7A9B5517662" modified="2016-07-06" version="1">MITFLDPSIGECRFLVGVTEYSKSLGCGLDNRWATGLLLIIWVVWVLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion outer membrane</location>
            <topology evidence="1 14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="14"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HCE6"/>
    <dbReference type="STRING" id="79200.A0A166HCE6"/>
    <dbReference type="Gramene" id="KZN10081">
        <property type="protein sequence ID" value="KZN10081"/>
        <property type="gene ID" value="DCAR_002737"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205222"/>
    <dbReference type="OMA" id="ERTDMGY"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01893">
        <property type="entry name" value="Miro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000935">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 14" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="314"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="422"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KZN10081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG83693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="644" mass="71865" checksum="2B6BE95042EF3515" modified="2016-07-06" version="1">MPRASNSGALRTAVRVVVIGDRGTGKSSLISAAASESFPENVPSVLPPTRLPADYYPDGVPVIIVDTSSSIQNKSRVAEELQRADAVVITYACDQRETLTRLSTFWLLELRRLEVKVPVIIVGCKLDMRDEGYHISLEEVMAPIMQRFREIETCIECSAANLVQVPEVFYYAQRAVLHPTAPLFDQETQTLKPRCVRALKRIFILCDGDEDDALNDAELNDFQVKCFNAPLQPAEIVGVKKVVQEKVPEGVNDFGLTLTGFLFLHALFIEKGRLETIWTVLRKFGYNDEIKLRDEYISIPLKRAPDQSVELTGEAMEFLKGVFSMFDNDNDGALRYSELDDLFSTAPESPWEESPYKDAVERTALDHLSLSGFLSEWDFMTLVDPARSLANLIYLGYNGDPASALHLTQRRLLDRKKKQTERNVFKCLVFGPKKAGKTALLNSFIGRPYSEHYFPTSAGSYAVNRVDRLRGNKKTLILQEIPEDGAKKFLSSRESLAATDVAVFLYDSSDEYSMKRAAELLVLVARRGEESGFGVPCLFIAAKDDLDSYPMAIKDSEMICQDMGIHAPISVSVKDGDMNNLFYRIVNAAEQPHIGVPETEIGKYKKRHRQILNHSLVFVSVSAAVTVVALAAYRAYAARKNASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HXN5</accession>
    <name>A0A166HXN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_003173</name>
        <name evidence="10" type="ORF">DCAR_0103356</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a SCF (SKP1-cullin-F-box) protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HXN5"/>
    <dbReference type="STRING" id="79200.A0A166HXN5"/>
    <dbReference type="Gramene" id="KZN10517">
        <property type="protein sequence ID" value="KZN10517"/>
        <property type="gene ID" value="DCAR_003173"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205111"/>
    <dbReference type="OMA" id="DKYTASM"/>
    <dbReference type="OrthoDB" id="7827685at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000057">
        <property type="entry name" value="SKP1-like protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="8">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="7">
        <location>
            <begin position="109"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN10517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="158" mass="18023" checksum="7E24E320E22808A2" modified="2016-07-06" version="1">MASESKMIMLKSSDNENFEVEEAVAVESQTIKHMIEDDCANTTIPLPNVSSKILAKVIEYCKKHVESPKTDEVNKTVEDELKTFDAEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENAWAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IQ05</accession>
    <name>A0A166IQ05_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IQ05"/>
    <dbReference type="Gramene" id="KZN11362">
        <property type="protein sequence ID" value="KZN11362"/>
        <property type="gene ID" value="DCAR_004018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="814" mass="91257" checksum="B47DD7A0AFFA6704" modified="2016-07-06" version="1">MAKKGSELPKAKGDRIEGNGNTSRVHFSKSRGGISSEEFESKTKPILQYWFLLETEMYEAIMKGNLSLMPVATARVNYLKKVIPANLLGEGIRGEEDALWRIHRILRNNGWWERAGNLVISEQSQGMPTNSDPLYQFLQLHSHLVHPNTRKLVAKGVREGIRMALNQIHYGSIEEARKKKTGLSPKDDTNVARPSKFPTSVTTIPSKIMVEFISSFRHLVEPSVYRDAMRGHDKALSLALGQIHHKTLPLDSQQVHKNSYKDILNLEREGSIKMDKSEPQQNMTPPSGKEITDTIFFTGMNSSVKVIEIWQHFKKAGKIRDIILPRKRDRFGNRIGFVIAHSVSEAEKIIRSLKGSKIGSSELYVAHAKKFSSASSSRKATSRGSTFPAQSPKKGLDKPNPHVVDSNCAKSKLPPYVVPPIRLEANEDLKSELDNCLCLITAKPETVESVEFIVEGLGFRDAVIRGMSSTKFLAYFDSLALISEEDLDFLSIGFMEVRKVKDTDLIPPRKVWLEFRGLPIIGWTEQNFAQLVKNWGPVISFGQILDKVDCYCLPKLLIETDCLSTIEEEVEVEISQKKWCITIKESHADELQYEVGPSVLPDIEKDISEGDLNGEVMDNAATHNSAHLNDRIMDDIISHNPDIEGDNISEINDTSDVEVEDSLLNPLTPRPSTSSDHIPTSGTRDQINQSDHSVEKVEIFQTANWKPREKESSISMVQMQSDQDKSTSDYNPVDDLSDLCSSHPSILKDLKNLKVQSKRGRPRKPSSKHLNKHFKVPRKKKHGVSVFTLLDSSFILAKFVGGNQAAKFPAQLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YCG4</accession>
    <name>A0A175YCG4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Cytochrome b6-f complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Cytochrome b6-f complex subunit PetN</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Cytochrome b6-f complex subunit VIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">petN</name>
        <name evidence="15" type="ORF">DCAR_0C036267</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="15"/>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 14">The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cellular thylakoid membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PetN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069424.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YCG4"/>
    <dbReference type="SMR" id="A0A175YCG4"/>
    <dbReference type="GeneID" id="88614240"/>
    <dbReference type="Gramene" id="KZM81215">
        <property type="protein sequence ID" value="KZM81215"/>
        <property type="gene ID" value="DCAR_032477"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614240"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Plastid Pltd"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009512">
        <property type="term" value="C:cytochrome b6f complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042651">
        <property type="term" value="C:thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00395">
        <property type="entry name" value="Cytb6_f_PetN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036143">
        <property type="entry name" value="Cytochr_b6-f_cplx_su8_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005497">
        <property type="entry name" value="Cytochrome_b6-f_cplx_su8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03742">
        <property type="entry name" value="PetN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103451">
        <property type="entry name" value="PetN subunit of the cytochrome b6f complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="15" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH16708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="29" mass="3170" checksum="2B1641516534C166" modified="2016-09-07" version="1">MDIVSLAWAALMVVFTFSLSLVVWGRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YEL2</accession>
    <name>A0A175YEL2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_029535</name>
        <name evidence="2" type="ORF">DCAR_0934023</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </source>
    </evidence>
    <sequence length="724" mass="82834" checksum="92D17997FD6D3F59" modified="2024-05-29" version="1">MLERDNVLVNIFRQLRERYTWGQPEPISVRLLERRKTDGHFENLPTTNDYEFAGLVVDDDFSSHRDIVGDNKKKGLEHISELHPSFMSLQYPLLFPFGEDGYRNNIKHRGANNSETQRNDTVSMREYYAFRAQYRTAEGRTLLVGGRLLLQFLVDACFRPGDVDATDVGKRIILPSSFTGGFRYMQQNYQDSLAVCKEYGHPDLFITFTSNPKWDEVQEVVRSSGSHDASVRPDIVARVFKMKLDAMINDFTKKHVLGRVLAGFKRCFILLASICCLHDRVSEARASHAHIVLWLAPADKLLTVDEIDDVICAEIPDKLTDQGPDTVTILLEIHGTQAPTTDIPSSSVKKLCVDEVRLRDNETLPEVVWRVDPDGTMFIQWMLNNRNDELGRDLTFIKYPTKFRWDASAKRWFRRKQNIEVVGRMVYAHLASGERFYMRLLLNLVVGPQSFEDIRTVDNIIYPTYKEACFHRGLLESDKEWHIALSDASLYANASQIRDLFVTLLVFCEISNPSELWEKHWAALADDIEYTRRKITKLPNLKLTDDDKQMLALEAINGLLKQYGKRIIFDRILESVLTNNGGFYFIYGPGGTGKTFLWSTIIATLRSEGKIVLPVASSGIASLLMEGGRTAHLRFKIPIDVNEFSCCDIKQNTHLAELICNTGLVIWDEAPMTNRFVFEVVDRTFRDIRSKINEESRLLPFGGLTMVLGGDFRQILPVIPRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W478</accession>
    <name>A0AAF0W478_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0102082</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W478"/>
    <dbReference type="KEGG" id="dcr:108227309"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000029">
        <property type="entry name" value="lysine-specific demethylase JMJ30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056520">
        <property type="entry name" value="ARM_KDM8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF105">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24472">
        <property type="entry name" value="ARM_KDM8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="250"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="406" mass="45550" checksum="6B56B1792D917236" modified="2024-05-29" version="1">MAAEIPGETFESPLLASHSPSLLHQISEHGGYAYVSMYTLGAGGDHRAAEAAREMAWEQLHSGPWNSVLPVWRDAYSMACLLVAKHCCAVGEYKEALRVLDLGVIMGGLTLRKDLDDAIAITKAKESGACSISEGNEGKRVLVDAEFNLKEMLQLLPKQSLSCKLVGKRPALSLEAFLRDYFLSASPVILSDCMVHWPARVKWNDINYLKKVAGSRTVPVEVGKNYLCTEWKQELITFSEFLERIQTNDSTSSVPTYLAQHPLFDQIPELRKDIVVPDYCYTGGGELRPINAWFGPAGTVTPLHHDPHHNILAQIVGKKYVRLYPASLSEELCPHSESMLCNSSQVDLDNIDEKQFPNIQNLEFMDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWSELENSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5Y6</accession>
    <name>A0AAF0W5Y6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0102799</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5Y6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="12"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="145"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG83622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="29741" checksum="0C194A26099AE045" modified="2024-05-29" version="1">MGDKMVSNCKDIVLRNSDIDTLKGPCFLSDQIIAFYFTYLSCSFNTKTDMLLVPPSISLWIANCEDTTCVEDYVRSCEMSSKRLVLFVVNDNEDFGGGDGGNHWSILVYDRTKNLFLHFDSMMGVNTPYAVKLYDALKEFMGPGGESSIPHTSSSLTKQQRKKKKSGPSVQRGSKPTQPPVTRESEATVVSGLPMFAECETTPQQQNGYDCGLYVLAIARAICQWCSEEHKRTDMISTIEKNVDSSVEMKMRSQVLEIIREMAADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W621</accession>
    <name>A0AAF0W621_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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        <subcellularLocation>
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        <text evidence="2">Belongs to the ARS2 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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            <end position="610"/>
        </location>
    </feature>
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        <location>
            <begin position="678"/>
            <end position="708"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="164"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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        <name evidence="3" type="ORF">DCAR_0102932</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7N3</accession>
    <name>A0AAF0W7N3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="10">C2 and GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">DCAR_0102327</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
        </source>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOG83153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VASt"/>
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    <dbReference type="PANTHER" id="PTHR46296">
        <property type="entry name" value="BNAA05G37250D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46296:SF8">
        <property type="entry name" value="OS06G0297800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2DOMAIN"/>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
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        <location>
            <begin position="260"/>
            <end position="432"/>
        </location>
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        <location>
            <begin position="528"/>
            <end position="646"/>
        </location>
    </feature>
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        <location>
            <begin position="884"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="852"/>
            <end position="871"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
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            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
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        <location>
            <begin position="852"/>
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            <dbReference type="ARBA" id="ARBA00004167"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7N5</accession>
    <name>A0AAF0W7N5_DAUCS</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ORF">DCAR_0103113</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </reference>
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            <begin position="1"/>
            <end position="66"/>
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    </feature>
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            <begin position="26"/>
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            <begin position="39"/>
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    </evidence>
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            <dbReference type="EMBL" id="WOG83934.1"/>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="66" mass="6522" checksum="32B6E11A846F8FA0" modified="2024-05-29" version="1">MNGAKRSAEAVGCKNTSVGERSALEGSTRASGSGRSGSENVGLSNANIGENPMPRKPKGSSAISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9R7</accession>
    <name>A0AAF0W9R7_DAUCS</name>
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            <fullName evidence="14">Heparanase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104686</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="heparanase-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF13">
        <property type="entry name" value="OS07G0598400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042290770" description="Heparanase-like protein 2" evidence="11">
        <location>
            <begin position="25"/>
            <end position="509"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG85497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="56428" checksum="F350E17D865734F7" modified="2024-05-29" version="1">MGSCMKAFSLFVFVFLTLAGLCVSETVDVKVRSVTSVAETDENFVCATMDYGPSSMCNYGQCPWPKVGILELDLKNKILANAIKAFNPLRLRVGGSLEDQTVYKVGLVTKCPHFKNNMNVRRFGMSNGCITMERWDQLNHFFNETGAKVTFGLNALRGKKLENKNSTLWVGNWNKLNAQEFMKYTISKGYKIDSYELGNELSASGVFARVNAGQYGKDMIILSNLVKKLYANFSDQPKILGPGGFYDKEWFNEFLRVSGPGIVDGLTHHIYNLGAGDDPKLINNVQDPFFLDEIAQTFKDISDSIKQYGPWTGAWVGESGGAYHSGGKNLGMASTFDHKVYCRQVLIGGNYGLLNTTTFAPNPDYYGALLWHRLMGPRVLQVSHDGSPYLRSYAHCSKDSERGDITVLLINMSNSTTFTVNTMNDLNLFPSEHTERLPLREEYHLTPEGGNIQSQVVLLNDTPLKLTKTMEIPNLNPALVLPLDPIKVAPDSFVFAVLRGFGAPACSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAH5</accession>
    <name>A0AAF0WAH5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205548</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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            <tissue evidence="8">Leaf</tissue>
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            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <source>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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            <end position="143"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
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        <source>
            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    <comment type="catalytic activity">
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            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
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            <topology evidence="1">Peripheral membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
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        <location>
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            <end position="519"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG89409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="768" mass="88285" checksum="06C29E90658B0CB4" modified="2024-05-29" version="1">MASEIEEVEDIAKPSERPVYLKKFNLYRTHSYFYMIGQDKSGAVRRVLKISRLEPFELDITEDSTTYSEVDCKELLKRIDDGNMSTGGLKFVTTCYGIVGFIKFLGPYYMLLVTKREKIGVICGHAIYSITGTDLFSIPNSTVLSNMAYSKNENRYLKLLCTVDLTRDFFFSYSYNIMCSLQKNMRDHEAGHFLYETMFVWNEFMTRGIRNHLKNTLWTVALVYGFFKQVNLQISGKCFNLILIARRSRHYAGTRYLKRGVTEEGYVANDVETEQIVITDAPDGCTQISSVVQNRGSIPLFWSQETSRWHMKPDIILSKKDPGYKATRLHFINLAKRYGNPIIILNLIKIREKRPRESVLCAEFHNAIDIINRDLRAENRLKFLHWDLSNFSRSKTMNVLLILAKLAEQALKLTGIFYCQAQSSSKRKEIFNWFSSENSERSNIIKASFQNGVLRTNCIDCLDRTNVAQFAYGLVAFIRQLNTLRLLDTPSIDLDSPLADELLRLYEMMGDELALQYGGSAAHNKIFCQRRGQWKAATKSQELFRILQRYYSNTYRDAEKQDAINVFLGHYKPHQGNLELWVLDSDQHPPNARNGRPYFLQENARSEILRSLSDGIVLSEAKSPNENANVSKEEKIKPVFPDKMQDSNKGHSESAPDIFTCKIQASYFRFTPGLPDRQLFPKNSEEDLFSSNFIDLDWNSQSENLYEEEMYDGYPVVNHDGLSSDNLVNELNVGGESGCSMQHDGECSSNILADFSFARMMEKCCSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLE8</accession>
    <name>A0AAF0WLE8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0310725</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLE8"/>
    <dbReference type="KEGG" id="dcr:108214789"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000066">
        <property type="entry name" value="Extended synaptotagmin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000102">
        <property type="entry name" value="Synaptotagmin-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF214">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="67"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="240"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="402"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="60947" checksum="A050E2630303679A" modified="2024-05-29" version="1">MGIVTSVMGIFGFGLGITIGLVIGYYLFIYFQPTNVENPTVPSLDEKDSKALKMLIQELPVWIKYPDHDRVDWLNKFIETMWPYLDKAICKTVKTIVQPIIAEQIPKYKIDSVQFEVLSLGSLPPVLTGMKVYTTAEKELIMEPSLKWAGNPNVLVAIKAFGLRATVQAVDLQVFACPRVTLKPLVPNFPCFAKILVSLMEKPHVDFGLKLLGADVMSIPGLYRFVQDLIKDQVANMYLWPKALEVQIMDPAKAAKRPVGILQVKVVRALKLKKKDFLGASDPYVKLKLSEDKLPSKKTTVKHKNLNPEWNEEFHVVVKDPESQVLEIVVYDWEQVGTHDKMGMNVVPIKDLLPDESKVLTLDLRKNMDPGDVQNEKLRGQLVVEAIYKPFKGSEIPLDTENSDEVQKAPDGTPAGGGLLVIIIHEGQSIEGKNHTNPSARLLFRGEVKKTKTVKKNRDPRWDEEFQFMLEEPPTNDRIHVEVVSTSSRMSLYPKETLGYVDIYLSDVIKNKRINERYHLIDSKNGMIQIEMQWRTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNC2</accession>
    <name>A0AAF0WNC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNC2"/>
    <dbReference type="KEGG" id="dcr:108214673"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000003">
        <property type="entry name" value="Exocyst complex component SEC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000002">
        <property type="entry name" value="Exocyst complex component SEC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="754" mass="85970" checksum="9BC218F194307378" modified="2024-05-29" version="1">MADDLAVEAKEAAMREVAKLLPTQEILASIASIKADYITRQQANDAQLSAMVAEQVEKAHAGLDSLSLSQNTVNQLRENFATIQKLCQECQTLIENHDQIKLLSNARNNLNTTLKDIGGMMSISVEAAAASDSLKNDKELINTYERLTALDGKRRFALAAASSHKEEVGRLSEYFEDVDRTWESFDKILWGHISNFFTLAKESPQTLVRALSVVEMQEILDQQLAEEAVEAEGDGAMASLASTRGASKRFTSSMTSSRNLTKQKQKVGKCYKDRCYDEIRKSVEARFNILLTELGFEDLKAAIEEAKAIGEELGDVYDYVAPCFPPRYEIFQLLVNLYTERFIQWLRLLSDRPDDLTNIEILKITGWIVEYQESLIELGVDETLAQVCSESGSMDPLMDAYVERMQATMKKWYLNILEADKVQPPRATDEGRLYTPAAVDLFRILGEQVQIVRDNSTDLMLYRTALAIIEVLDDFQAAQRKRLEEPASEIGLEPLCAMINNNLRCYDLSMELSTSTIEALPENYAEQVNFEDSCKGFLEVTKEGVHQTVLVIFEDPGVQELLVKLYHKEWFEGQVTEYLIATFGDYFSDIKMFIEERSFRRFVESCLEETVIIYVDHLLTQKNYIKEETIERMGLDEELLSEFFREYITASKVENRLRTLTELRDLASSESPDSFTLVYTNILETQPDCPPDVVEKIVGLREGIPRKDAKEVVQECKEIYENSLIDGEPPKRGFVYSKIKCLQQHKGYLWRNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNV7</accession>
    <name>A0AAF0WNV7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0310684</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the APS1/VSP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNV7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07535">
        <property type="entry name" value="HAD_VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010028">
        <property type="entry name" value="Acid_phosphatase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014403">
        <property type="entry name" value="APS1/VSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01675">
        <property type="entry name" value="plant-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF7">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042240159" description="Acid phosphatase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="267"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR002674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG91435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30719" checksum="7D8F2858913F828D" modified="2024-05-29" version="1">MYTSLRLLLFSSLIYVSICQETFDPHTLPRPLILETHVKETPLLSSADDDVTLHCTSWRFAVESNNLSPWRTIPQECGDYVKDYMTKRGYEVDLERVADEAILYASSVELRGDGKDIWVFDVDETLLSNLPYYADHGYGLEVFDSEAFDKWVDNGIAQAIRSSLKLYEEVVKFGFKVFLLTGRSEMRRMVTVENLNRSGFHGWDDLILRGAEDQKKTATQFKSEKRHKMIEQGYRIHGNSGDQWSDLLGSAMAARSFKLPNPMYYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPY8</accession>
    <name>A0AAF0WPY8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0313208</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPY8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044159">
        <property type="entry name" value="IQM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250:SF10">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="36619" checksum="4A2690C883E5418D" modified="2024-05-29" version="1">MKVIFSIFTTVLPQYHSAYRFRTRFMPKFGVESFAFTPSKLVDGFLRMESSHSKHNKCYIFCINFCKSETDASRWNQVSLNASKVGQGLSVDAKAQILALQHWIGAIDPRHRYRYGLHLYYEEVCIKYLRTQEREHYEYVVEEKIVHQQTGTFLITNNGLPGAKWISVMSTCLNFLAGRALAAAGRLSISSYNGHYRPTDERLDSFLHFSRIMEKLGRAGSVEYTISESLQPDNIPKVEDTIPVHEETEVFEPKNNYSKTLSGGLQSPVANVPKKSILERINSKKAAKSYQLGHHLALKWSTGAGPRIGCIADYPAEIRPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WR24</accession>
    <name>A0AAF0WR24_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0311848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WR24"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34558">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34558:SF9">
        <property type="entry name" value="F3L24.15 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042174858" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG92575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15132" checksum="8583D363571DF6F1" modified="2024-05-29" version="1">MARLIITFLIFSHPLIFLAMARQSDTVQNFSSSSDSNLQKPAESPASRKLGKHSNELIKSYADAPHVSPSEEPTIKAQQVHSNKEIYMKKPLLHHRSGAKSVAGGGVILGSLATTFLIAILLYIRATRRNSANQTPGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WX21</accession>
    <name>A0AAF0WX21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Glycoside hydrolase family 19 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0415597</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WX21"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF79">
        <property type="entry name" value="CHITINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041924060" description="Glycoside hydrolase family 19 catalytic domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 19 catalytic" evidence="9">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 19 catalytic" evidence="10">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="43"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="116"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="262" mass="28396" checksum="AB9D2CEF7590454C" modified="2024-05-29" version="1">MGGLYGLVVILLWTVSVRGQDVGSVISKSMFEDFLKHRNDDICPAKGFYTYEAFIDAAKSFGAFGTSGDPDTQKREVAAFFAQTSHETTGGGPSLPDGPYAWGYCFKEEQNPTDYCVADPEWPCVPNKNYHGRGPIQISYNYNYGPAGKAIDYDLLGNPDLVASDPTVSFKTALWFWMTPQSLKPSSHDVITGVWKPSEADSAAGRVPGYGVVTNIINGIECGKGSNSGGEGRIGFYKRYCSILGISPGENLDCYNQKPFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYV8</accession>
    <name>A0AAF0WYV8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0417825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EMC8/EMC9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYV8"/>
    <dbReference type="KEGG" id="dcr:108219400"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08060">
        <property type="entry name" value="MPN_UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005366">
        <property type="entry name" value="EMC8/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941:SF10">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 8_9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03665">
        <property type="entry name" value="UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="2">
        <location>
            <begin position="7"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="204" mass="22586" checksum="F9348EBC91D4E2F9" modified="2024-05-29" version="1">MGGEFRYEIGQNAYIKLILHSLKHSSSAVNGVLLGRVSGDANSVVEISDSVPLFHSQIGLLPPLEIALLMIEEYYSRQGLSIVGYFHANERFDDFELGGVAKNIGDHIYKYFPQAALLLLDNKKLGALSKGKDRSPVMQLYTRDASRTWKFIGSDGSSQLALKEPSANVVLQDYISTGKAENITDFDDHLDDISKDWLNSGLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZZ3</accession>
    <name>A0AAF0WZZ3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417024</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the auxin efflux carrier (TC 2.A.69.2) family.</text>
    </comment>
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                <person name="Senalik D."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DExH box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2K6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000526">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000860">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF213">
        <property type="entry name" value="3'-5' RNA HELICASE YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="R3H" evidence="10">
        <location>
            <begin position="10"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="183"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="535"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="986"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1099"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1145"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1099"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH00010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1192" mass="132778" checksum="5D6AD6F204131E35" modified="2024-05-29" version="1">MHTFWLVQHTEIVSDDYIWQSKLLPWNSQMITNYRFDVSLTRAERSEVHLLCSKMGLASRSSGSGRGRRITVYKRRVRAKKIRENNITRFTLSEEAKDVSLNLFTRYPPYDCGSGDKMVGKFVEKTSRRQGPDPFFSRPLMSKFDIKRKVELLTNKLESDPKLKKITEKRTKLPIASFRDAITSAIDSHQVVLISGETGCGKTTQVPQYVLDHVWSKSKTCKIVCTQPRRISAVSVAGRIASERGESIGNSVGYKIKMESEGGRNSSIIFCTTGVLLRLLVNMGSNNLGKESSKTRDAITGPDGITHIIVDEVHERDHFSDLLLAIVRDLLPSKPNIRLVLMSATIDAERFSQYFGGCPVIRVPGFTYPVKTFYLEDILSILKSNENNHLESASRLVTSENILLSEEYRAVIDDTITLAWSNDEYEPLLDLVTAEVGFNILDFQHSTTGVTPLMVFAKKGRVGELCMTLSLGADYSLQDNDGATALEWAERENQKEAAEILKKHMEKQVANTEQKECLLDQYLANVNSELVDFVLIEKLLKKICTDSTDGAILVFLTGWDDIQRTIERLKRNSFFSDASKFLLLALHSKVPSIEQKKVFERPPPGCRKIVLSTNIAESSITIDDVVYVINSGRMKEKSYDPSINVSTLLSSWISKASAKQRAGRAGRCQPGICYHLYSKIKEASFLDFQVPEVRRMPLEELCLQIKILDPARKIGEFLEKLLDPPATQSVTNAISILEELGALSPDEELTELGNKLGSFPVHPVLTKMLLFAILLNCLDPALTLACVSSYRDPFNQPMDPIEKKKAISAKLELASLYGGHGDQLAVIAAFECWKIAQEKGQGASFCSRYYLSPGIMKMVSRMRKKFLSELYRKRFIPKDGPCCSLNAHDPGIRNAVLLAGFYPMVGKLPLNKRGQKGIVETAGGVKVRLTSSSSNYMLASDGSEPLIIFDEIIRGDAGLLVKNCSVIGPLPLLLLAKEIMVAPCEEDDKNKGGTAPEFSGKDTEDDNEVHKHKDSPVGQTIMSSPSNIVKVVVDRWLTFESTALDVAQICCLRERFSSATLFKIRNSFGTQSKIADDSIQSVARILSYDGLSGIPSTLKYKNQQTSETSAPEAEQSELVREKGTSSQDFCLTSLMQQYHSQNCMRHQGDEEPHHGLHHGGKASGSMKRDKSDQPGYCHKQLPPSELTIPKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8N2</accession>
    <name>A0AAF0X8N2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0623191</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8N2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000003">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-0229">DNA integration</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="17">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="18">
        <location>
            <begin position="767"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="196"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH03791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1138" mass="131585" checksum="A69CC695C5E08601" modified="2024-05-29" version="1">MSQPQVVRRNAPVKRNYKELRKLGATDFYGTVDPTEAESWLKRTKRVFNMMHCGDEEKFDYAVSLLQDDAYDWWETVPDSDAQPPKLTWDDFQREFKDKYMPEIYRDEKQREFLNLKQGNMTVAEYEVKFTQLSRYASSMVATERDKCRRFEEGLKYEIRSKITIGELRSYTDLRAAAIRAERLIKEKPAFFSKSKREGTRFAGEAGGRPKKRPNASAPTKSITRGGFSGSQGGRLNNPRSNPTLSRGSNVQVKPTCQTCGRQHFGQCRAQTGGCYLCGEQGHFIRDCPNKRENVQAVSEPSVQNTAFRTRYGHFEFLVMPFGLTNAPAVFMALMNKVFQPFLDKFVIVFIDDILVYSKSKSEHEEHLRTALQILRENQLYAKLSKCEFWLDHVVFLGHVISSKGIEVDPKKIEAIWNWEVPKNVTEVRSFLGMAGYYRRFVEGFSKIAGPMTKLLRKNVPFQWTEEAQQSFDELKRRLTSAPVLTTPSGQGGFVVYSDASQQGLGCVLMQYGKVIAYASRQLRPHEKSYPVHDLELAAIVFALKIWRHYLYGETFQIFTDHKSLKYLMSQKELNMRQRRWVELLKDYDCTLEYHPGKANIVADALSRKCSSVANLQGSTFPSLVELRKMNIGLEVDTCGVLLATLNIRPVLKERIQKAQINDPKLRDAVERVRQGQETRFTLYEDTLMLGNRICVPNDEDLRREILDEAHNAPYAMHPGATKMYNTMKSHYWWSGMKRDVAEFTAKCLTCQQVKVEHQAPAGKLHPLSIPEWKWEKITMDFVTNLPKTRKGNDAIWIIVDRLTKSAHFLPIRWGCTLDHLAQRYVNEIVRLHGVPISIVSDRDPRFTSRFWKSLQGAMGTRLNFSTAFHPQTDGQSERTIQTLEEMLRACVIEFKGSWDEYIALMEFAYNNHFHSSIGMAPYEALYGRKCRSPLYWDKEGTIILEGPELVQNAVDKVNIVKAKLKATQDRQKSYVDQNRREMEYQVGDKVFLKVSPWKGVMRFSNKGKLSPRYIGPYEIIEKIGPLAYKLALPPELSQIHDVFHVSMLRRYRSDPTHVLKDPGIEINDNLSYIEEPVKIIGHKTKQLRNREIPLVKVLWRNHAVEEATWETEEHMRSKYPHLFDNPGTFLNFEDKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8Q3</accession>
    <name>A0AAF0X8Q3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0521921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8Q3"/>
    <dbReference type="KEGG" id="dcr:108223129"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016135">
        <property type="term" value="P:saponin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1540">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH02532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="459" mass="51289" checksum="E80AFADDB81CD8F3" modified="2024-05-29" version="1">MVEEPRPAHCLVFPYPMQGHINPMHQFSKTLVSKGLKVTLVTTKFMFNSLQQLSGSMPIETVSDGYDVGGKYAAESIEVYLDKFKQVGSETLTQLIEKLNATGVPVDCIVYDPLMSWVLDVVKSCGLVGAIFFTQSCAVDNIYYHVKQGVIKVPVVEDRVSLPGLPVLGPLDMPSFVHNPGSYPATYNLVVNQFSDIDKVDWVLCNTFYKLEEEVIQWMSKHLRLRAIGPTIPRKYLHNQKTAEDDTDNGLQMFTPNIDSCMKWLNEQPDSSVVYVSFGSVAQLDAAQMQELACGIKQSKKQFLWVVRASEEAKLPKGFAEETSAQGMIVHWCSQMDVLAHKALGCFVTHCGWNSTLEALCLGVPMVAIPIWTDQGTNAKYVADVWRMGMRVDVDKNGVFTREMVQHCIRVVMDGEKGKEIKMSCRKWMEMAREAVKEGGSSDISINEFVASLTQQCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XA90</accession>
    <name>A0AAF0XA90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0622725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XA90"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040256">
        <property type="entry name" value="At4g02000-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025558">
        <property type="entry name" value="DUF4283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31286">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31286:SF180">
        <property type="entry name" value="OS10G0362600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14111">
        <property type="entry name" value="DUF4283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4283" evidence="2">
        <location>
            <begin position="283"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH03329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="700" mass="75484" checksum="C412A716C41D806D" modified="2024-05-29" version="1">MDDFPPLPPPGLVAPMVPENALVVLDEMPLAVAAPVDALAMPVVGAALEDVQAVPDVGAALEDAQAVPDVGAVPENAPVAPGPGAMVHFQAVFDNLSDEESVHTNDMYVNAPPGTLTRLRQDFAIMDAGGTIRDHSHTQSESSSSIGVRIDNHPEIIKANRFKLDYVNFLKSKGLLDDFNAYQSSLSNLHARKVDDNGVIRPTLASVARDFCVRQGILCVGNSNPDVGTSAPVAPKDAKTWSSIVAPFVPQNDSSTPEPSLSYNVDGSANLSPPKSFLLAGRKRWATSCIGYFVGGGFSFKFVKENALKMWKNRGLLNVYFNSKGYYTFEFKTEDEMKSFLGASSMMMGGKRMYLGAWIEGTNFQRNVIPFVSIWVQFSDVPHSYWTAEGIRMLARAVGKPITLDAPTAKLEPMRYARVLVEVSYKSPKPDFIWVPVISDEDGSIVKVKVQVLYSAMPLSCSLCACYGHSLARCTKNPNKVMDPSSDKGSTRAPTAHHGAPKSDTPKSTTRAPPEHTELQQQNGNTQLEDVEMIQHMADIITDLARDMAYTQVEHTATDSVQTPVPENAPVALEGILESAPLDAAFADQEQINSPNRFQALADVDDDVMIDNTGIHNSYAHGPNVVVFGLAAPGGAMALPPLPPALEEGEFTPASKGKKKQRSKVQQASPSKRGLGNGDKGSTLPKRSRRLQGDATKASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAM3</accession>
    <name>A0AAF0XAM3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroflavonol 4-reductase</fullName>
            <ecNumber evidence="8">1.1.1.219</ecNumber>
            <ecNumber evidence="7">1.1.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydrokaempferol 4-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0623787</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="8"/>
    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAM3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
        <property type="term" value="F:flavanone 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF809">
        <property type="entry name" value="ANTHOCYANIDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="14">
        <location>
            <begin position="6"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH04378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="343" mass="37547" checksum="225E3F1EAFF8BBB5" modified="2024-05-29" version="1">MEKTRVCVTGGSGYLGSWLVKKLLHKGYIVHATLRSLDEKSKVGLLKSLPNANTGLVLFKADIYDPNDFQAAIEGCNYVLHLATPLQHNTKSSLYKDTREAAIAGVKTIADCCLKSQSVKKLIYTSSVMASSPLKDDGSGFESYWDESCWTTINDTPFTYCDDFAYTMSKTSAEKEILSYNATASDPDTGLEVVSLVCGLVGGDTILSYLPASMSTIISPLFGDSLNSYYQSLQHLQELLGCVPLVHIEDVCEAHVLCMEKPSLKGRFICSNADPTAKEITEYFKNNNPNSELEILDDIIGERGSRCSSIELIKLGFEHKFNMADILDGSVHCGKMLGSLPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFZ8</accession>
    <name>A0AAF0XFZ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0625788</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFZ8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH06362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="175" mass="19526" checksum="53AC72275BB4D509" modified="2024-05-29" version="1">MSVLVARTGRQQQRYQDGYRLLAGCIPFKYRDSEEGSGVLSEKIVEVLMITSNSGPGLLFPKGGWENDETVEEAATREALEEAGVRGDLMHLLGDYYFTSKTLQDESSPAGLCKATMYALFVKEELDLSEADECCRHSWMKKALKEGFSGWLADGMMSTMKDRNNLHSPTNIDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XK99</accession>
    <name>A0AAF0XK99_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0729174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XK99"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841:SF7">
        <property type="entry name" value="CYSTEINE_HISTIDINE-RICH C1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10707" checksum="999071AB7847FBF6" modified="2024-05-29" version="1">MHQGHALLQIHNTQDYLCGGCKTNGEGTNFGCESCNFHMHDFCSNCPKILSSFLHPQHPLSLMISSNTQFSATPRICDVCEETRLRVCFTGVKFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMR8</accession>
    <name>A0AAF0XMR8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase MuDR plant domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0729347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF187">
        <property type="entry name" value="MUTATOR TRANSPOSASE MUDRA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="85"/>
            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
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            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="55111" checksum="F24C58B4F2651327" modified="2024-05-29" version="1">MENDLFELETDEELMLMCTLITNKERPKINRYVELFVSRICAPLNVELDGDWAPNQEEIHMGNPFPDSTQQEIDLEQELRDFEGLDKDFSDPADSEDYELNGSDSDDETWHSDASNKDDTDDDLIFEKNIDKTVFEEHDIPEEEPEKDDSVHTDDDSVYAGSDEERMAANSTDEEDDDFHVFNEDVDMERPVFKNGMLFKNSQVFRKAVRTHAIVERRPVELVPNYGRKVKYKQINSVWLAEHYEKEIRMNPSWPVAAFHKKIFNDFKCEVSKHAVYRAKTRALLKINGTHRAQFAQVWDYAYELKMILPDSTVKILCEDPEPGTERGRFMRIFCVMHLYQNMHKEFKGIALRQLLWKAARSTTNWEFNLHMNKMKEIASRCHEWLMAKPKSQWTRSSFRTLVHSDMFVNNHCEVFNSSIRKYRDLPIITMFRELHKSVMKRIQVRRDKMVARDTVICPNALKKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMT9</accession>
    <name>A0AAF0XMT9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="18">Plasma membrane ATPase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0729388</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cell membrane</location>
            <topology evidence="2 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="18"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMT9"/>
    <dbReference type="KEGG" id="dcr:108193770"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 18" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="751"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="817"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH09927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="949" mass="104716" checksum="1813246AB8C392AA" modified="2024-05-29" version="1">MANLEGIKNETVDLESIPVDEVFEQLKCTRQGLTSEEGQQRLQIFGPNKLEEKKESAFLKFLGFMWNPLSWVMEIAAIMAIALANGDDRPPDWQDFVGIVVLLFVNSTISFIEENNAGNAAAALMAGLAPKTKVLRDTKWSEQDAAILVPGDVISIKLGDIVPADARLLDGDPLKIDQSALTGESLPVNKNPGDEVFSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTNNVGHFQMVLTAIGNFCIVSIAVGIIVEIVVMYPIQHRKYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHKLSEQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLTVDKNLVEVFAKDMDKDTVLLYGARASRVENQDAIDACIVGMLSDPKEARAGINEIHFLPFNPVDKRTAITYTDSSDNWYRISKGAPEQIIELCNLKDDVSKRAHSIIDKFADRGLRSLAVAQQTIPEKNKDSPGGPWVFVGLLPLFDPPRHDSAETIRRALHLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSSLLGQHKDEAIAHLPVEELIETADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIIHAVLTSRAIFQRMKNYTIYAVSITIRIVLGFMLIALIWKFDFSPFMVLIIAILNDGTIMTISKDKVKPSPLPDSWKLKEIFATGIVLGTYLAVMTVIFFYAAHDTDFFSDNFDVKSIRDKDHELISAVYLQVSIVSQALIFVTRSRSWSFVERPGFLLMGAFIIAQLIATFIAVYANWDFANIKGIGWGWAGIIWLYSIIFYIPLDLLKFLIAYTISGKGWNNLIDRRIAFTNKKDYGRGEREAQWALAQRTLHGLQPPNTSELFTDKTNYRELSEIAEQAKRRAEVARLRELHTLKGHVESVVKLKGLDMEGIQQHYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPC2</accession>
    <name>A0AAF0XPC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0729948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPC2"/>
    <dbReference type="KEGG" id="dcr:108196905"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd03407">
        <property type="entry name" value="SPFH_like_u4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000013">
        <property type="entry name" value="Hypersensitive-induced response protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF36">
        <property type="entry name" value="HYPERSENSITIVE-INDUCED RESPONSE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="5"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH10479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="286" mass="31554" checksum="3BFC044977F8E78C" modified="2024-05-29" version="1">MGNLFCCVQVDQSTVAIKEHFGKFDDVLDPGCHCLPWVFGYQIAGHLSLRVQQLDVKCETKTKDNVFVNVVASIQYRALSEKANDAFYRLSNTRSQIQAYVFDVIRGTVPKLNLDAAFEQKNDIAKAVEDELEKAMSHYGYEIVQTLIVDIEPDEHVKRAMNEINAAARLRMAATEKAEAEKILQIKRAEGEAESKYLSGLGIARQRQAIVDGLRDSVLGFSVNVPGTTAKDVMDMVLVTQYFDTMKEIGASSKSSAVFIPHGPGAVRDVATQIRDGLLQGSAAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPT3</accession>
    <name>A0AAF0XPT3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0729834</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPT3"/>
    <dbReference type="KEGG" id="dcr:108195387"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA-ATPase ASD, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="244"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="489" mass="56441" checksum="5A8346A3DCC0C7B2" modified="2024-05-29" version="1">MRATTKTIMSTIGTIAATAMVARSLARDYVPPEIQDYFHLGLRQFFRRFSNQLTMVINEYEEIESNEIYDAAVVYLGSKVSPNTRRLKVAKLKQENNISLTMERNEEVIDTFNGVEFKWTWVCQQLEKSNTYYNDHQTRSFRSEFRFFKLSFNRKHKEQAVNSYLPFILKEAKDKEQEFRTLKIFTLHSDYLFSSPDKWQSVSLDHPATFETLAMDVQLKETVLKDLERFVARKDKYKRVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLELTDLKKNSDLRKLLIATANRSILVVEDIDCNIELQERVSAESEDRKPHHKEKNRVTLSGFLNFIDGLWSSCGDERIIIFTTNHKEKLDPALLRPGRMDVHIHMSYCTPCGFRLLAGNYLGVEDHKLFREIEELILTTEVTPAEVAEQLLKSDEPDVSLASLISFVHTKKKENEEAKGKKATEEVAALVTEAAKDCEKGDQNQESEKIQKPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQ00</accession>
    <name>A0AAF0XQ00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0730313</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQ00"/>
    <dbReference type="KEGG" id="dcr:108195886"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000025">
        <property type="entry name" value="Protein phosphatase 2C 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="14">
        <location>
            <begin position="203"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH10839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="531" mass="58043" checksum="4384A12518EDF6AC" modified="2024-05-29" version="1">MEEIPPAIEVTVSLSSSICDNSGISSNVEITHVQIVTESSNMLPDPVSVLRAESVSSRERSFNCLKRTLSGTVESERKISVSETILNGDSSWVPTDAMIQQNKEDVADDCSGIKSKQLLALNASTGMSLPIAVEIEEIDNGQIFAKVISLEKQSVEGKMSASADDVSSNILTLKSSVIALQLPKEKITGCEESKGVHMKYVPLWGHVSICGKRPEMEDAVVAIPQFLEVPTKMLLSDHVINGMSQNAGVITSHFFGVYDGHGGSQVANYCRERIHFALKEKLEGIEDFESEVRTAVTQQEQWEEIFTSCFQKIDDEVGGKVSQTETGGDCDTSNGNLEPIAPETVGSTAVVALLCSSHIIVANCGDSRAVLYRGKEAMALSNDHKPNREDEYARIEASGGKVIQWNGHRVFGVLAMSRSIGDRYLKPWIIPKPEVMFVPRAREDECLILASDGLWDVMTNEEACEVARRRILLWHRKNGPVTDSDRGQGVDPAAQAAAEYLSMLALQKGSKDNISVIVVDLKAQRKFKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR92</accession>
    <name>A0AAF0XR92_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0831001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XR92"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH11514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="43" mass="4742" checksum="ACAE6928DCC845F4" modified="2024-05-29" version="1">MDSFSNSASVLNVVRKKLSRCSSICPVLMDEAGRGIQGCRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRB3</accession>
    <name>A0AAF0XRB3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0831348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRB3"/>
    <dbReference type="KEGG" id="dcr:108198239"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="37"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH11852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="20448" checksum="DC9DB79EC825429B" modified="2024-05-29" version="1">MGCSVAIFITIHTTHIPFVPFVLVFSLIMLKKMMSIFLLRLLALGATISATVVTLTTHDSRHVLNLTFKASYSNAPTLRYFVVANAIASGYSIIVLFIPSKNSLWKFVLISDVIVTSMLVSSISAALGVAQIGKKGNSHAGWLPICDQVPKFCDRLTGALIAGFVAPILYLLLVLFSLHNLINLLSAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTE4</accession>
    <name>A0AAF0XTE4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTE4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000013">
        <property type="entry name" value="Extra-large guanine nucleotide-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053057">
        <property type="entry name" value="XLG_GTP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36486">
        <property type="entry name" value="OS01G0977800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36486:SF4">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="640"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="747"/>
            <end position="750"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="865" mass="97935" checksum="783CE30F93F53C7F" modified="2024-05-29" version="1">MASVFKRFVPEKRLGSDVDDEYNSEYSFATEYTGPLVSYDVPCVAPVHVDRIPIAASIATPDRTKNMALPVVQPVARVDSGKLSKKSKKGLLGDASSMSVTHSNELDVARVLDVSKRHRTDFRVVNVLDTSGELEFLETEECIHRNLNRCASSGTLGFSGSHETSCELSESSGMESLHEDKYNGASFKGHEQEAVKCTVRKGSCHRCLVKIRFREKEVCIVCSAKYCSSCVLRAMGSMPEGRKCITCIGCRIDESKRSTLGKSSRMLRRLLTDSRAKEIMSHEISCAVNQIPPELVIVNGKPLCHEELVQLLGCPNPPRKLKPRKYWYDKQSGLWGMEGEKPCQIITPQLAVGDPIMKNASNGNTNILVNNREITKPELWIMQFAGIRCEGQPHFWCSADGSFQEEGHGNVKERIWFKPKIKLLCSVLSLPIPPESANSDQGKVDDKVVFSTQKRNEPYKLLMVGYEKSGTSTIFKQAKLLYKIPFTEDERQNMKNIIQSNLYRYIGILLEAREQFEEETLNEMRMKFINQPGLSDINQIDKANIYSIGPRLRGFSDWLLQIIMSGNLTAVFPASTREYAPYVEELWKDKAFQATLDRRNEIQTLPRVANYFLNRAVEISSMYYEPSDMDILYAEGIAASNGIASMDFSFSKSKKDSILDSNDEDDPLISYQLMRVHSSNLGKNCKWMEMFEDMDLIIYCVSLTDYDEYCSDINGVSTNRMMESKKLFETIVTHPICSHKNFLLVLNKYDLLEEKIEQVPLTRCEWFRDFNPLISGQQSSRSSSSKNPVATLAQSAFHYIAGKFKRQFQSLTGNKLYVFRVTGLESDSVDEAIKYATEILKWENEKPNFSMNDWSSGSIEATTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWB1</accession>
    <name>A0AAF0XWB1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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            <tissue evidence="3">Leaf</tissue>
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    </reference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="388"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="687" mass="78524" checksum="E44A6B152AAD7D00" modified="2024-05-29" version="1">MESQRVYVKWREVFVLSERGRKEVRYYLKRRDGVSDLVVVGKERRDLKVYRYNIRETSLVGQADFKLKSRKDVIEWLNSVISVPVGGVSEVISDCQPSSQAKDGQLQKPGKHGTEVLWVGSATALKKKRKHYQSFQRNGVKISVHDFVYVLAEEDKPLVAYLDDMYEDSRGNKMVVVRWFHKIDEVDIALPETYNYNDREIFFSLCLQDLNIECVDGLASVLSPQHYQVFLREARHTRFEPFICGMQFENDDVKPFDITQVKGYSEQKIFDYMLPTSPSTNAENFLAKDGLKLDVECNENFTVKPKKRLRLSNKYGACLQPANKSQGLYDATRMDPKLTTGSNDNIVGPDLSVLKQSVPPVSLPPREVVMQKSKALTPGVQIEVQKSTAFSVGGQIEVLCQDSGVRGCWFRALIIKKKSDKVKVRYLDLKDAADEVNNLEEWLLASRAAVPDEWGFRISRRKTVRPAPVHSKHGGEKGGDGLIIKDGTVVDVWWHDGWWEGIVIQKESEDSIHVYFPAEKRESVFCWKDLRPSQEWLGTGWKQMGERQDLLTSMLPKLDRKPDTLIPCQSVVCIDKLQLGFDQEGKLSNSNNTDDKYVARDIVKDDLLEKLKWKTSGKRKRSPNSSRKPFCSETVNRNRVRAFGTRTWEKFFISSSAKVDHENCKNTRESAFSSPVVSPLSNLVLSR</sequence>
</entry>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="243"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="27"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
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        </location>
    </feature>
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            <begin position="55"/>
            <end position="95"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="51225" checksum="0256988A3DFC55B3" modified="2024-05-29" version="1">MVYNKVYGVPLYGSSWLPLNLINSADDHDHDHDHGSPSKSQHDDDINDNDPLTKPQHDHDDDPLIKSQHDNNDDPQTKSHHDHTNSRDDRDHDDVAVEESPPSIKPTRHVVLSGGGGEGRIGIPNALLISQFDPNTKSLSDHPVAELGTGADLPSRMAVHPKGEGVMCSMPKSCRWYEWEAIERDGVHGWSPKSSEKIFKQLEDVGRQLALTFNEEGSVLATGGEDGKLRVFKWPTMDSILDESGAHASVKDLAFSPDGRFLASVGSSGPGRVWDIASSAPIASLKTKKDEQFVLCKFSQSLDNNHQILYVTAMQGKGGSIVKWKAKSWKPKSWKRISSKLVTRDSITAFSVSADGKYLAVGTMEGDILVLNSSSMLVHSVIKKAHLGILTSLMFSEDSRALLSTSFDSSARVTLIDDEKMVTIINDQKIKGWNFWIILFILFLAIAVYYVRLLEFLVLLEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWT4</accession>
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            <fullName evidence="4">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ORF">DCAR_0935173</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15630.1"/>
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                <person name="Macko-Podgorni A."/>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKH5"/>
    <dbReference type="KEGG" id="dcr:108195189"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000006">
        <property type="entry name" value="Pectinesterase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF158">
        <property type="entry name" value="PLANT INVERTASE_PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042036145" description="Pectinesterase inhibitor domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="8">
        <location>
            <begin position="26"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG83792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="20757" checksum="08CE6B830111F6D2" modified="2024-05-29" version="1">MMSHFITFLLSLTVLTLLTYSASSENGDDYVQDACSVTRYRNLCIRSLSPFSTTAKRNPTTWARAGVSVTISEAKRVASHLVKLSSSGGRYKGALQDCVEVFQDSLDNLHKSLYVLRELNGQTFTSQMSDVTTWMSAALTDEDTCLDGFSGHKGRQIKSLQNRIRNVSYITSNALALVNKLATTGVSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKH9</accession>
    <name>A0AAF1AKH9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0102650</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKH9"/>
    <dbReference type="KEGG" id="dcr:108221772"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF41">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042139761" evidence="9">
        <location>
            <begin position="24"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="348" mass="37238" checksum="7ACF570DCB545079" modified="2024-05-29" version="1">MAIHIKNHAHFLIFLIIISTVSSISGASNTTAECTRPIGGCHDKPYAQKLKIIAIFAILITSMIGICLPIFTRAVPALSPDRNLFAVVKAFASGVILATGYMHVLPDSFDALRSECLPDRPWHKYPFTTLVAMFSAVLTLMVDSFAMSLFKRHYALESGEQKEVSNESVELQSHCHGHGVAAEGSDATLLRCRVVAQILELGIIVHSVIIGLSLGASNNPCTIRPLVAALCFHQMFEGMGLGGCILQANYRAKTNAIMVFFFSATTPFGIILGLVLSNVYSDNSPAALIVEGLLDAASAGLLNYMALVDLLAPDFMGAKLQAHLKLQALCYVAVVLGAGGMSVMAIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALG3</accession>
    <name>A0AAF1ALG3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0104042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALG3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF236">
        <property type="entry name" value="OS02G0749800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG84857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="389" mass="45171" checksum="735DEF86AFB2B00A" modified="2024-05-29" version="1">MFKSHLQTLIINHRTLPNLNHCLIRPIFRSYSPKRTIDSSEFKISFLKNKCGLSGKHLIRASKCVHFDSSDHRPDSVLELFRNFGFPQPNITRIVSVVPRVLQSLKPEELLKPKLDFLLSMEMSHAMVVRIVTRDPRILVRSLNKHLIPTFDQLKDIIGCGPNAVALLKCGPFIFTNTAEVLPNIRFLLRQGIPISQIFKFVSQYSRILCYPHDRFCQVFLMVEGMNFDYSSSYFIHALHALSFHNDSTWESRCLLLRSFGFSNDEILKVFKKSPLIMCYKENHINKKVEFFLKKLQWTPSRLSCNPKVLCYSLEKRTIPRCSVLQVLVSISSTSKSYNISTILAIAERKFVRDFVSVYKDELPEVMEAYKGKMKFDEYTFKQKGLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMF8</accession>
    <name>A0AAF1AMF8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0207839</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMF8"/>
    <dbReference type="KEGG" id="dcr:108208700"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07858">
        <property type="entry name" value="STKc_TEY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="41"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="374" mass="43004" checksum="BE8C6690ACDE25BD" modified="2024-05-29" version="1">MESSSAALADHTHIKGVPTHGGRYVQYNVYGNLFEVSRKYVPPIRPVGRGAYGIVCAAMNSDTREEVAIKKIGNAFDNRIDAKRTLREIKLLRHMNHENVIAIKDIIRPPQIQSFNDVYIVYELMDTDLHQIIRSNQQLTDDHCRYFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEVMTRQPLFPGKDYVHQLRLITELIGSPDDASLGFLRSDNARRYVRQLPQYPKQQFSARFLNKSPGALDLLEKMLIFDPHRRITVDEALCHPYLAALHDINEEPVCPHPFSFDFEQPTCSEENIKELIWKESVKFNPDPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQG6</accession>
    <name>A0AAF1AQG6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0208383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQG6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF42">
        <property type="entry name" value="BACTERIAL SURFACE ANTIGEN (D15) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0934">Plastid</keyword>
    <keyword evidence="1" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="4">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG89147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="233" mass="25003" checksum="8FE913469C74BC57" modified="2024-05-29" version="1">MEEITTRDENTNICSNGPSLFSAQRLGAGPPTTLSGTGTDRMAFLQANITRDNTKIVNGATVGERNVFQLDQGLFIGSKFPFFNRHQLTYTKFLQLKAVEEGAGKSPPPVLVLHGHYGGCVGDLPSYDAFTLGGPYSVRGYNMGEIGAARNIVEVAAELRVPVGNTHVYAFAEHGNDLGSSKEVKGNPTEAYSRMGQGSSYGIGVKVGMVRTEYAIDHNSGTGAVFLRFGDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASD4</accession>
    <name>A0AAF1ASD4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASD4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786:SF74">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786">
        <property type="entry name" value="DNA BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="3">
        <location>
            <begin position="201"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="839"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="899" mass="104171" checksum="135033A958F8C6B9" modified="2024-05-29" version="1">MTSLGVRVDSAINRRRGPFVFRASGVNIHFIGSLIPEKDQRPKYAQLYIYDTENEVRNRIATMQKNGPNEDIDEEIVSQISRMLDEKNHLVESFRKARDRYKNGPETSFQLRIPGNRAQDGRQYDMPTSSEIAGLIVGDIDKKNYKRDVIVHHKTRGPEQISDLHPSYMAMTYPLIHPYGEDGYRLGITLRDGKNLTMCQYYCFRLQQRLNEGHTLLRSGRLLQQYIVDCYMTIEEERFRWIRLNQNKLRSDLYSGLMDAVHRGDSNCSRVGKSIILPSSHTGGPRYRVQNYQDAMAICKWAGYPDLFITFTCNPKWPEINYMLELIGQKDDRNRLDIICRVFHIKLLQLMQDLKKEKPFGKIIASLYTIEFQKRGLPHAHILLFLDPVMKNPTASRIDEIVSAEIPDFNVDPDGYNAVKNFMLHGPCGKLNKNSPCMANGKCTKHFPKKYNDKTTFDSDGFPIYKRRNTGIHIKKNGKILDNRFVVPYNRNLIVKFDAHINIEVCNYSRSIKYLFKYVNKGPDRANATFESIDTVERIDEIKAYLDCRYISAAEACWRIFQFDINHRHPSVERLPFHLPGEHTIIFEEDKCVDNVLNRPGVDKTKFTEWLETNKIHEDARQLTYSDFPTRWVWNAKEKIWTRRKKGISVGRIYFAHPSSGERFYMRMLLNFVKGSTSFECIRTVNGVTYKTYKAACYALGLLDDDKEWIDCLNEAAGWATGNELRNLFVTILIHCQVSDASHLWKNCFIALSEDITSLHRKRFNNKELKLTEEQVEAYTLVELETLMQKSGKSLRDIDGMPQPNTSLLSDSSNRLVNEELDYDTAKLKVQHENLYAALNTCQKVAYEAILKSVENDEGRLFFISGHGGTGKTYLWNTIISNLRSRSQIDSPFTFPYPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASP7</accession>
    <name>A0AAF1ASP7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0313110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASP7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48210">
        <property type="entry name" value="OS05G0352800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48210:SF1">
        <property type="entry name" value="OS05G0352800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG93823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9403" checksum="B1FFA0C84D630AF5" modified="2024-05-29" version="1">MTNDKGNVEGKKQAAADVLYNYSSFAMTCIGNQVRPCDLRMHLMKEISGMQTSLKESSQKPGSPKAGTASSSGMQLDKNETFRALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASR9</accession>
    <name>A0AAF1ASR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0313171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASR9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056889">
        <property type="entry name" value="NET2A-D/KIP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056888">
        <property type="entry name" value="NET2A-D/KIP1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631:SF0">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25014">
        <property type="entry name" value="NET2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24918">
        <property type="entry name" value="NET2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="4">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="NET2A-D/KIP1-like alpha-helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="NET2A-D/KIP1-like C-terminal" evidence="5">
        <location>
            <begin position="685"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="173"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="582"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07765"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG93883.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="913" mass="104043" checksum="68B3BCD3504872FD" modified="2024-05-29" version="1">MLQRAASSAYSWWWASHIRTKQSKWLEQSMQDMEDKVEFALSLVSEDGDSFAKRAEMYYKNRPELINFIEEAFRCFRAMAERYDSLSTDLQKANTTIATCLPEQIQFAMDDDDDFLASKHPKFSSGPAADPSKVPKAPARPMKGMMHAATKKLEPKKSSIPPRRIKPKSGLSKAEAVEEIDKLQKDILSLQTMKEYVKSSYESGISKFFALENDIMEKQDKVSRLQDEFGVSTMIDDNDARTLMAEAAIKSCKETLADLQNKQENSAEKAEVENKKIEDVCQRLESIKYELLPDQTDNVQRVDQNSNHGAEEFQSLKQEMIGPERKKLEALREKIKEHKEGSGGPLTVSELADKIDDLVNLVISLESAVLSQNVLIERLRTEADDLQSQIRRLEENKGGLMETHELQSRVKELEEKLYLVQNIYMNITTQNNDIKDQFAEAHSSLNDLSEKLPSVEPDEELEETDLLEEEEHGLIEKRPEKALIEQDKGKSGDEALLEHDKGKGGGKALLEQGKGEGGGTALIKQDKEEGGGQALIEQDKGKDGGEALIEQDKKEGGEKFEDELNWQQMLLHGVEDKERILLAEYVAILRNYKEVKKKLSDEEKKNETLFETMLQLRNTKSALLKKDQEIQFLRHKLNLLKQDSCDIPGAKDSTEDESFGMHPTEEDKEEIKLLINQPKTLSPIEEKLRGQIDAVLEENLEFWLRFSNSFHQVEKFKTEVHDLQDEIVQAKKKNELRQDGKSQGSISADLKAEIRAIYKHLSEKKTEVGVWLEQSELLKEEVQHRITSICEIQEAITNGLKEGVESDEITFSSHQAAKFHGEILNMKQENNKVNEELLAGIDHITALKIEIEKTLVKINAEFKLTERKPSSSTSSSRSYSISKIPLRSFLFGIKARKHKHSIFSYRHYSRSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATJ8</accession>
    <name>A0AAF1ATJ8_DAUCS</name>
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            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0313855</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterodimer with UBC35 or UBC36.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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    <dbReference type="CDD" id="cd23807">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 1C"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="6"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="156"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00065118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG94559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="156" mass="18016" checksum="22338C99D25FA1F2" modified="2024-05-29" version="1">MYLYIYIYIYIFGGGGGGLTWLPRNFRLLEELERGEKGIGDGSVSYGMDDADDIYMRSWTGTIIGPHNTAHDGRIYTVKLFCDKDYPDKPPTVRFHSRINMTCVNHDTGLVEAKKFGMLANWQREFTMEHILTQLRKEMTTSQNRKLAQPQDGTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1ATN2_DAUCS</name>
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            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <lineage>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10818" checksum="980E173234548B1F" modified="2024-05-29" version="1">MEGLKRAVGDAMGKSEYGSSSSSSKVVTAAANISSSPSSSSSYDRSAQLLNRSHRQVVSVWTCSKLCAFCFVAGILVGFTLKRRVRRWASRLLRRLKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWY3</accession>
    <name>A0AAF1AWY3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0414844</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GEM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AWY3"/>
    <dbReference type="KEGG" id="dcr:108215595"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037848">
        <property type="entry name" value="GEM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="3">
        <location>
            <begin position="95"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="221" mass="24108" checksum="4621B1455575B105" modified="2024-05-29" version="1">MGGAAAAPTSYPNNESGGGAEYHQPNPYVHYSVVPKPPANPMDTVVQKLNIWSSKAEKTANNIWHNLKTGNSVSESAWGKMNLAAKAITGAGFEQLYKQSFSTFPNETLKKSFACYLSTTTGPVAGTLYLSNFHVAFCSDRPLSFTAPSGQTTWSYYKVLIPLGKVANINPVLMSDAEKYIQIMTVDGQNFWFMGFVNYEKASHHLIESVSHFAALGIPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX72</accession>
    <name>A0AAF1AX72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0415652</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AX72"/>
    <dbReference type="KEGG" id="dcr:108218109"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041999099" description="WAT1-related protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="14"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="185"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG96317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="364" mass="40265" checksum="0FBD1C11CDCE1DF2" modified="2024-05-29" version="1">MGKHMFFSDKMKLLIALLTLQCCYAGFHIVSRVALNIGVSKVVYPIYRNIIALLLLGPFAYFYEKDQRPPLTFSLLVQFFLLALVGITANQGFYILGLYYASPTLASAMQNSVPAITFVMASALRIEKVNILRRDGLAKILGTIASVGGATIITLYKGPPLLHNQQSQQPLEVHSLANKTQNYTWGCIYILGHCLSWAGWMVFQAPVVKKYPAKLSLTSFTCFFGLIQFLIIAAFFERDPKNWQIKSGEEIFTILYAGIVASGVVISLQTWCIYKGGPVFVAIFQPVQTVLVAIMAFIVLGDQLYSGGIMGAILIVIGLYTVLWGKSEETRVENSLKEDTLTKHLLDPVERQAEECAVGVTDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B008</accession>
    <name>A0AAF1B008_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417984</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B008"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000043">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000021">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF387">
        <property type="entry name" value="F8L10.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="132"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="442"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="579"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG98640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="660" mass="73977" checksum="3FC73938A86B11BC" modified="2024-05-29" version="1">MGVNCCKPSAIEDSRESPRERLSSKASSDIRVGRVTSSRQEEGCRGKDRLDGYDGRAMLIDKQVNGSIRLNGEHFERKREKSEYAIAMNHPGSGRILRATEGEQVAAGWPPWLAGVAGEAIRGWIPRKADSFEKLDKIGQGTYSNVYRARDLDKGKIVALKKVRFDNLEPESVRFMAREIHILRRLDHPNIIKLEGLVTSRMSCSLYLVFEYMEHDLSGLASHPSLKFTESQVKCYMQQLLRGLDHCHNCGILHRDIKGSNLLIDNNGILKIADFGLASFYDPRQSQPLTSRVVTLWYRPPELLLGATYYGTAVDLWSAGCILAELYAGKPIMPGRTEVEQLHKIFKLCGSPSEDYWRKSKLPHATIFKPQQPYRRCVAETFKDFPAPALALMETLLSIDPADRQSAANALRSEFFSSKPLPCEPSSLPKYPPSKELDAKLRDEEARRQAAGGNKVPRYDAERKVKRESRAVPAPDANAELVLSMQKRRGQSNSRSEKFPQEEVASGFPIEPPRPSQAVEEEANDPQGNLHKRASHSGPLVHRASWTKVGKNMDDPPKISTGAELSSVNSLVAARRSLISEDRRDKPGYLQQETPKVIGRFPGSFKESSDSGRKQDHMPSVTGQPHEDGRNNNKDPILVSSLHQSIDPTFHEYLLLFLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B298</accession>
    <name>A0AAF1B298_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0622853</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B298"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="14"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH03455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="163" mass="18915" checksum="472FB2BEEEFAB266" modified="2024-05-29" version="1">MNKLRPKNLLPLSLHEAFRLFCEGGSHYGPYWDHVLGYWKASLEFPDNVTFMKYEDMKKEPLFSLKKLAAFLGKPFSEKEEKQGVVQEILQLCSFDNLSNLEVNKTGILRYSSQIFVNNSYFFRKGEVGDWQNYLTDEMVKHLDSITKHKLKGSGLTFGSPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B658</accession>
    <name>A0AAF1B658_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4228 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0727088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B658"/>
    <dbReference type="KEGG" id="dcr:108194436"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19414" checksum="9122DB6272B84615" modified="2024-05-29" version="1">MGNYVSSCTPISSTMKSPKAARVIFPSGEIRQFREPLKAAELMLECPSFFVVNSKSLNVGKRFSPLSADEDLEFGNIYILFSMKRVNSMVTGGDMAVVFMAASSAPKRLAGVNNNKGRVLPETAESVDRSPENDEGSRLNLDEVEGFSTVEIRYRLAVCRSKKPLLETITEEPLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B927</accession>
    <name>A0AAF1B927_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Malonyl-CoA decarboxylase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0728689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B927"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050080">
        <property type="term" value="F:malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001294">
        <property type="term" value="P:malonyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.90:FF:000002">
        <property type="entry name" value="Malonyl-CoA decarboxylase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.150:FF:000002">
        <property type="entry name" value="malonyl-CoA decarboxylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="entry name" value="Malonyl-CoA decarboxylase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.90">
        <property type="entry name" value="Malonyl-CoA decarboxylase, oligemerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035372">
        <property type="entry name" value="MCD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038351">
        <property type="entry name" value="MCD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641:SF1">
        <property type="entry name" value="MALONYL-COA DECARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17408">
        <property type="entry name" value="MCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA decarboxylase N-terminal" evidence="3">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Malonyl-CoA decarboxylase C-terminal" evidence="2">
        <location>
            <begin position="205"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57129" checksum="105A937027099E9D" modified="2024-05-29" version="1">MNRKGLAILLRTKMRPLSPAINSTNQIQFPARETRDRNQESSRDREFDQVKVSMHSAISMDEKENVDAALNDFSAGYFRLSRDKHKILLHLLAKQYDVYRIQVRELMKQYLDIQLPSGEIAEDGSYTEEAPLNSFYRIERNLKDALKPMYEVLFERLNTHPGGLKFLTIIRADILSILAEENIPSLRALESNLREKLITWLSPANLELHQITWDDSASLLEKIVHYEAVHPISNLLDLKRRLGVGRRCFGYLHPTIPGEPLIFIEVALMKNVAQTIQEVLWDEPPIPEPEATCALFYSISSSQPGLAGINLGKFLIKRVIDVVRKDMPNISTFATLSPIPGFLQWLLPKLASADTFQENMLDPEEETALLDLSMEFTAGKSGMEIMLTLLTLNNYEWTKSDDLITALRAPMLRLCARYLLQEKKRGKALDSVANFHLQNGATVGRVNWMADRSEKGLSQSGGIMVNYIYRLDKIEENAQAYFSTGHIEVSPDDETKHDQPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BB33</accession>
    <name>A0AAF1BB33_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0730006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BB33"/>
    <dbReference type="KEGG" id="dcr:108195895"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47580">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47580:SF1">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH10537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32167" checksum="7AD15E22F0EA8D0E" modified="2024-05-29" version="1">MIITRTFASTTIPPKILNPPPPLPDQLTRRHILTSLTISVTPFFTPPQPHLSTPPSHARGLFQMPPPRLTNRYFLVRAGESEFESVGIINTNPVAKTSMDNGLSEIGKKQTIRAALALREMGACDRSCWIWPSITQRAYQAAEVIAAVNKINRSNIVPEYSFLDARGLGAYEGKNLESISEIYASDNLSPNIKPPPTNDGTPNESVADVFVRVTQLMSILETQYSEDTVIIVSPDSDNLTILQAGLVGLDLRRHKELYFSPGEVRFVDTSSIPTYKQPASAVYKCLNPPSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD72</accession>
    <name>A0AAF1BD72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0833153</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BD72"/>
    <dbReference type="KEGG" id="dcr:108197092"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="5">
        <location>
            <begin position="208"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="592"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="599"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="624" mass="69781" checksum="3C218C9AC0BFA1CE" modified="2024-05-29" version="1">MKFMKLGSRPDTFHTTDGVRSVSSEIVTDLIVQVEGNRYLLHKFPLLSKCLHLQQQCFEAPESSSQQEIIFPEFPGGIEAFELCAKFCYGITITISAYNIVSARCAAEYLQMTEDVEKGNLINKLEVFLNSCVLHGWKDSIVTLQSTKTFHAWAEDLGITSRCIESIVSKVISNPSKVNLSHSYSRRGKDDALSCNGSESRSKNMSTGWWAEDIAELGIEIYWRTMMAIKSSGRIPSNIVGDALRIYAHRWLPKISRAFNNEKQVESSLESDSVAEVASMPRLFLKSIITLLPVDRTAVSCSFLLKLLKAANILRVSSSTKIELAKRIGTQLEEATVGDLLIPNMSVSCKEQYDVDIVITILEQYMLQGQSPPTSPPRSKGDIHRRSRSAENISELHESRRSSSASHSSKLRVARLMDGYLQVVARDANLPLLKFIAIAEAIPEFARLNHDDLYRAIDIYLKGHPELTKSERKRLCRVLDCKKLSAEACTHAAQNELLPLRVVIQVLFFEQARAAVATGQMTNIPGNIKALLAAHDDPMRPLEPSISGKTLQTDDQWSVSGLKTPSSNVSTLRMKLEEDDDLDESYVSGFGKSSQVKATRSHPNRPRRMFSKLWSINRQGSEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDN2</accession>
    <name>A0AAF1BDN2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0832716</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDN2"/>
    <dbReference type="KEGG" id="dcr:108199218"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000163">
        <property type="entry name" value="Geraniol 8-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF14">
        <property type="entry name" value="CYTOCHROME P450 76A2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH13207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58073" checksum="E75A95D06BF4D7C8" modified="2024-05-29" version="1">MEWNWNYVYWSVALMSLISLVGHLRRRNSYRPSKLPPGPRGWPVFGNLFDLGSLPHRSLEAFKQDYGPVVWLNLGSVKTLVLNSAAPVEELFKNHDLSFVDRITNDAMRSHDYYKISIAFGQYSSYWRTLKRICTSELFANKRIKETMLIRQDSVDEMLLWIEKEAAKGSSSGIVIRDFVFPALFNMIGNITMSQNLVDPESTIFSEFCAALGGFHKCLGRPNISDLLPWLRRLDLQGIRRNMDSDLGKAIGIISVFVKERVEQRQRKLEFSSEQKDFLDVLLDYRGNGKDEPAELSDFQVTIFLMEMFIAGTYTTTSTTEWAMTELLQNPEQMKKIKAELARVVGADKKLQESNIDDLHYLQAVVKETLRLHPPATMLLPRKAVQDTTFMGYNIPKNTQVLINNWAIGRDEDIWENALSFKPERFLDSGINYKGQNYELLPFGAGRRMCPGLPLADRMIPLILGSLIHHFEWELCDDGKVIDMRETLGSASQKLEPLQAFLKPKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y3B9</accession>
    <name>A0A161Y3B9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0727281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y3B9"/>
    <dbReference type="Gramene" id="KZM86649">
        <property type="protein sequence ID" value="KZM86649"/>
        <property type="gene ID" value="DCAR_023783"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196875"/>
    <dbReference type="OMA" id="MISDYNT"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08295">
        <property type="entry name" value="double_bond_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="343" mass="38168" checksum="36243D6F90E56983" modified="2016-07-06" version="1">MDETVDNKQIVLKEYIRGYPKESDMATQVSKMSLKVPHGSNGIVVKNLYLSCDPYMRSRMTKSTAVYVEAFTPGQPIVGYGVAKVVDSGTPKFKKDDLVWGMTGWEEYSLITNTDTLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEVATPKKGEYVFISAASGAVGQLVGQFAKLFGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPDGIDIYFENVGGKMLDAVLLNMRPHGRIAVCGMISQYNLENPEGVHNLTQVIVKRLRVEGFLVFEYYHLYPKFLEMVLPYIKEGKINYLEDTAEGLESAPAALIGLYSGRNIGKQVVVVARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y3L3</accession>
    <name>A0A161Y3L3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KAT8 regulatory NSL complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NSL complex protein NSL2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-specific lethal 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0727420</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Non-catalytic component of the NSL histone acetyltransferase complex, a multiprotein complex that mediates histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sites and promotes transcription initiation. Required for NSL complex stability and for transcription of intraciliary transport genes in both ciliated and non-ciliated cells by regulating histone H4 acetylation at 'Lys-5'- and 'Lys-12' (H4K5ac and H4K12ac). This is necessary for cilium assembly in ciliated cells and for organization of the microtubule cytoskeleton in non-ciliated cells. Required within the NSL complex to maintain nuclear architecture stability by promoting KAT8-mediated acetylation of lamin LMNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the NSL complex at least composed of KAT8/MOF, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y3L3"/>
    <dbReference type="Gramene" id="KZM86769">
        <property type="protein sequence ID" value="KZM86769"/>
        <property type="gene ID" value="DCAR_023903"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194495"/>
    <dbReference type="OMA" id="KYCHAHI"/>
    <dbReference type="OrthoDB" id="677315at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026316">
        <property type="entry name" value="NSL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025927">
        <property type="entry name" value="Znf_KANL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13453:SF1">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13891">
        <property type="entry name" value="zf-C3HC3H_KANSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="KANL2-like probable zinc-finger" evidence="15">
        <location>
            <begin position="151"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="98"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH07985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="284" mass="31394" checksum="232171EE771AE32B" modified="2016-07-06" version="1">MASSSNNFPNSANPNPKLNDSQMASDPINNSPPHIIDPDAALSQSEYLTRREVLSRRSRKLHELTNIYKDQYWAMMEELKNQFGEYYWEYGKSPFVEDEDGVENNGNANVNAEGENNNNNNLEIGEGEEDGDKGAGKGSGSGSGVLSNNRCAVQGCKMKAMPLTQYCHMHILRDEKQVLYKGCTYVIKSSQAGPILCGKPILRTSVPSLCTPHMQKAEKHVARALKKAGLSGHSTSKLAPQFHVIVAEFVNQIQAKRRAARDVKKEPGEVKEPCEVNEDDDISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y4U9</accession>
    <name>A0A161Y4U9_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_005144"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287">
        <property type="entry name" value="OS06G0551500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="16216" checksum="6C62BAB159A32A46" modified="2016-07-06" version="1">MPFISEISAAASSSCSLPVNVANTNPCFSPIRVEIVSKSVSRRLLHKFRDVSDAGLDYLPSRLWSPPFQPSMFLSSPGNSIVTPKDMLLRLRKLAQPPQRRRYRICFNVCYYVHIFLRTYSLNQIMKVIEICLFSCAGILVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YL17</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG82606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A161YL17"/>
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        <property type="protein sequence ID" value="KZN09127"/>
        <property type="gene ID" value="DCAR_001783"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060">
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KZN09127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="368" mass="41556" checksum="B21CBFBBBD17F2DC" modified="2016-07-06" version="1">MEGPPATMPAPLLLLRNIVTSFFLYADKSLLNLEHRYKFLASLRYILISAFFFLLNIFSHEYSKNVSKTSKNIPAARTYPGDTGISRALTQLLSIMNDVPVSSRKYEIVRTLSEKVLDGNMSECHAPLEEINVVVLSAAFSRTLLQLESRVAVIEDDSEIIEGAKGNFSKLSRVARAVWYCLEAAWSRMGMSEVNKGGKSAEKLAAEVLWLAQKLAQCGNVEEAVERWGSANKLAWLALSAEPRLQGSLVKVSVFLLKQAKDIGNDKEDDEGNRERHKQTKIKLLMSWLPLLCRATNGTDTPLLSFSEKGEIERVLEVIIEALNNEEQEKILSLWLHHYIHCPTSDWPNLQDCYSRWCNSSRRLLILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008643</name>
        <name evidence="3" type="ORF">DCAR_0209762</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZGF8"/>
    <dbReference type="Gramene" id="KZN07806">
        <property type="protein sequence ID" value="KZN07806"/>
        <property type="gene ID" value="DCAR_008643"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210271"/>
    <dbReference type="OMA" id="EDCHIIR"/>
    <dbReference type="OrthoDB" id="44820at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094:SF2">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN07806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="32045" checksum="C1F5090B3D3B9E90" modified="2016-07-06" version="1">MASIIQSPTISPVDRRRESPSNSAIIREAGTWKTRRAKSLICSKLKDVEAITVSELGDNLRLYGQFSSLVKETSEEEKEKQKYYVNMGDAIRTLREEFPHLFYRDLTYQIYRDDIVFKDPLNTFIGIDNYKSIFWALRFHGKVFFKALWVDIVSVWQPVESTIMIRWTVHGIPRVPWDSRGRFDGTSEYKLDKDGKIYEHRVHNIALNAPPRFRVFDVNDLLQSIGCPSTPKPTYYEIASLSSIKNIKLFEKESGSQHYLSSIFKDSGKAEAGISQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZVB0</accession>
    <name>A0A161ZVB0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_021835</name>
        <name evidence="12" type="ORF">DCAR_0624365</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="WOH04953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF12">
        <property type="entry name" value="OS05G0426100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM90800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH04953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="585" mass="64808" checksum="B7C4FD19BBB7A2E1" modified="2016-07-06" version="1">MSQSKIRQAIGAVKDQTSISLAKVASSTALADLDIAIVKATRHEQYPAEERYIREILSLTTYSRSHIAACVGTISRRLNKTKNWVVALKALILIQRLLSDGDPAYEQEFFFATRRGTRLLNMSDFRDTTKADSWDYSAFVRTYALYLDEQLECRMQDKRGKGNMFAYIEEDEEANQGATTARATPVQELTNGQIFSRIHHYMQLLERFLACRPTGAARTNRIVIVALYPIVKDSFQIYYDISEATGVLIDRFMVLDIPDSVKVYEIFCRVSKQYDELDSFYEWTKSVGVVRTSEYPDVDKITQQKLDAMDEFIREKSVMLRNRMAMNAEVEPVEEKEEEPEEDLNAMKTLPPPEGFVEETVKAEAVEVKVEEPAQEIKVEPKRQDVGDLLNLGADAPTSEEQSNRLALALFDGGPSTATPTVGTSAWEAFNNTGDWETALVQTASHLANQKSSLPGGFDTLMLDGMYQQGAIKHAMASSGMIATGSASSVALGSAGRPAMLALPAPPTAGGGAQSTSGADPFAASLGVPPPAYVQMSELEKKHQLLMKEQLMWQQYARNGMQGEVSLAKLQQQNAYPYNNGGYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZY28</accession>
    <name>A0A161ZY28_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_016467</name>
        <name evidence="4" type="ORF">DCAR_0518821</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZY28"/>
    <dbReference type="STRING" id="79200.A0A161ZY28"/>
    <dbReference type="Gramene" id="KZM93222">
        <property type="protein sequence ID" value="KZM93222"/>
        <property type="gene ID" value="DCAR_016467"/>
    </dbReference>
    <dbReference type="OMA" id="INVAHRE"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF91">
        <property type="entry name" value="PROTEIN LATERAL ORGAN BOUNDARIES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="13"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM93222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="118" mass="13271" checksum="90E7DAB13657C691" modified="2016-07-06" version="1">MAASFNVVRYDYSPCAACKLLRRKCLPDCIFAPYFPPEELQKFISVPKIFGASNVAKLLNEVLPHQREDTVSSLSYEAEARLRDPVYGCVGTISILQLQVERLQKELDEANAELVSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AXL9</accession>
    <name>A0A162AXL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0209198</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in the degradation process of specific misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Endoplasmic reticulum membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AXL9"/>
    <dbReference type="Gramene" id="KZN07303">
        <property type="protein sequence ID" value="KZN07303"/>
        <property type="gene ID" value="DCAR_008140"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205924"/>
    <dbReference type="OMA" id="FKSQYWR"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000016">
        <property type="entry name" value="Derlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="27930" checksum="E35F8FDD9F12D898" modified="2016-07-06" version="1">MAEAVEEWYKQMPIITRSYLTAAVVTTIGCSLEIISPYNLYLNPRLVVKHYQFWRLVTNFLYFRKMDLDFLFHMFFLARYCKLLEENSFRGRTADFFYMLLFGATVLTGIVLIGGMIPYVSESFAKIIFLSNSLTFMMVYVWSKQNPFIHMSFLGLFTFTAAYLPWVLLGFSVLVGASAWVDLLGMIAGHAYYFLEDVYPKMTGRRPLKTPFFIRSLFADDPVVVARPANVRFAAPPVEEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TDY2</accession>
    <name>A0A164TDY2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-glucuronate 4-epimerase</fullName>
            <ecNumber evidence="10">5.1.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0727833</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UDP-alpha-D-glucuronate = UDP-alpha-D-galacturonate</text>
            <dbReference type="Rhea" id="RHEA:11404"/>
            <dbReference type="ChEBI" id="CHEBI:57635"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="5.1.3.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.6" evidence="10"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TDY2"/>
    <dbReference type="Gramene" id="KZM87401">
        <property type="protein sequence ID" value="KZM87401"/>
        <property type="gene ID" value="DCAR_024535"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196659"/>
    <dbReference type="OMA" id="FAINYPP"/>
    <dbReference type="OrthoDB" id="202470at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050378">
        <property type="term" value="F:UDP-glucuronate 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000198">
        <property type="entry name" value="UDP-glucuronate 4-epimerase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="98"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH08394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="443" mass="48982" checksum="B3E7F1C07543F87A" modified="2016-07-06" version="1">MAFSPDTSKTTKVERYNSYIRRVNTTKLLAASSKVLFRATLLISIVLIFFFTINYHPLSNNSITSPHRHLSAFYGSGAAWEKQVRHSSTPRRPNGFSVLVTGAAGFVGSHCSLALKKRGDGVLGLDNFNTYYDPSLKRARQKLLSTHEIFIVEADLNDAELLAKLFDIVPFTHVLHLAAQAGVRYAMQNPQSYIKSNIAGFVSLLEIAKAADPQPSIVWASSSSVYGLNTENPFSESDRTDQPASLYAATKKAGEEIAHTYNHIYGLSLTGLRFFTVYGPWGRPDMAYFFFTKDLLQSKGINIYKTQDDKEVARDFTYIDDIVKGCVGALDTAEKSTGSGGKKKAPAQLRIYNLGNTSPVSVGKLVSILESLLNVKAKKHVIRMPRNGDVPYTHANVSLAYHDFGYKPTTDLSTGLRKFVKWYVSYYGIQARVKGESAAKNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TUW8</accession>
    <name>A0A164TUW8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TUW8"/>
    <dbReference type="Gramene" id="KZM88005">
        <property type="protein sequence ID" value="KZM88005"/>
        <property type="gene ID" value="DCAR_031528"/>
    </dbReference>
    <dbReference type="OMA" id="TIRTSWT"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="11276" checksum="97E6253AC1229D1F" modified="2016-07-06" version="1">MAMLCSCGMVAVQRTSWTDNNYARRFLGCVYGAKGCNYFRWVDPPSCPNCCHVIPGLLRKIEVCEEKVIKLEHDKKKLKKWCLIVFVCVVSMAVSRLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UFX1</accession>
    <name>A0A164UFX1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_025909</name>
        <name evidence="3" type="ORF">DCAR_0729762</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UFX1"/>
    <dbReference type="Gramene" id="KZM88834">
        <property type="protein sequence ID" value="KZM88834"/>
        <property type="gene ID" value="DCAR_025909"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195307"/>
    <dbReference type="OMA" id="MMARKSY"/>
    <dbReference type="OrthoDB" id="755797at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37708">
        <property type="entry name" value="HOMEOBOX HOX-B3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37708:SF2">
        <property type="entry name" value="HOMEOBOX HOX-B3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM88834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="206" mass="22573" checksum="90B3D94E55E4E706" modified="2016-07-06" version="1">MAALKSHNLEPQFTIIDPTSIILSQFQSDHNLLHLKTESIFLERGPNYKAYADLRESKLRSKNKFPPLDQEPPLTPPRKQVKFQGSLIPGPPRRKVSSVLAQSVPDFAAVVRKENRKPMMPMTPVIEKARTPPAGSKSGRMYGVGSKSCGSKSVNSGEKRNGGGLMGRKSYASMEELKGLSYAAANAINNGGEIRGGRRTILGYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VT98</accession>
    <name>A0A164VT98_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0624397</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VT98"/>
    <dbReference type="Gramene" id="KZM90825">
        <property type="protein sequence ID" value="KZM90825"/>
        <property type="gene ID" value="DCAR_021810"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192827"/>
    <dbReference type="OMA" id="YVTMFLI"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="447" mass="49990" checksum="05671CC7EAE3AF06" modified="2016-07-06" version="1">MYVVPPPKRSSDPMPGYSGGSSDNVRIYQIWKGSNIFFLQGRFIFGPDVRSLGVTILLIIVPVTVFCAFVARKLLDDFSYHSGVSIMVVAILFTIYVLGLLLLTSGRDPGIIPRNAHPPEPEGYDGSAEGGAQTPQLRLPRIKEVEVNGITVKVKYCDTCMLYRPPRCSHCSICNNCVERFDHHCPWVGQCIGLRNYRFFFMFVSSTTLLCAYVHVLCWVYIKRIMNSEDTTIWKAMIKTPASICLIVYTFIAVWFVGGLTAFHLYLISTNQTTYENFRYRYDRRANPYNKGVMQNFAEIFYNSIPPSKNNFRAEVPREPGLPPRSVGGGFVSPNMGKAVDDIEMGRKAVWGDVGDALDHREGQHPYTDSVNKDGGLGQMSPEIRTTVEEGDRVGIHHPRRSSWGRKSGNWEMSPEVLALSSRVGEANRTGGSSSSNLTTSQQAQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YDU9</accession>
    <name>A0A164YDU9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_017611</name>
        <name evidence="5" type="ORF">DCAR_0520191</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YDU9"/>
    <dbReference type="STRING" id="79200.A0A164YDU9"/>
    <dbReference type="Gramene" id="KZM94368">
        <property type="protein sequence ID" value="KZM94368"/>
        <property type="gene ID" value="DCAR_017611"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221499"/>
    <dbReference type="OMA" id="MVEDECH"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47591">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47591:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
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        </location>
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        </location>
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            <begin position="204"/>
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        </location>
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        <location>
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            <end position="600"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="631" mass="68905" checksum="B6A11721FC2909C3" modified="2016-07-06" version="1">MESGPSHQEQSSNKFLIKLKMPVKMDHHGDQEVATIQHVTNNTPRVCPQCNKEFGSGKALGGHMRIHAEAIRKQQRRQKMVGKLKDEEDGVGFQRLMNKKAVCNLCGKNFPSMKSVYGHMRCHPDRDWRGIQPPDEVLVENLDQRISDEDYEEEDDGSVGGGDCGPVGDLLADIPKWGEKARRGQKGVRKSSVEAVGSSGVMVSEEEDEEEEDDEDEDEFEDEDEEERLRNAVNDLMLLKQVDVSGSSGVSTLAAAAEISRDVNAGLDQNQEGSYGTSLTVNNRGEGSGNVEFRASGFVQGGYKEMKRSFGMVEDECHDHLVMEKMRMGEGGSDDHVIKNMSDHHMGQEANAAHHVVENYGDYDSRRTELMVMNNGGHKDFDWANHKEDNGEMVMGSSKNKKAKKMKLMMDLDQEKASAAGSSAQGMVSGSTTTEKYRCSTCNKSFSSHQALGGHRSSHNKFKLTIINGDSGLNNQSSSVNADRHRAGNEGFDQNLHQLITPGMENWVMSQDCANKKDSTIEGAGSSKGDHSSSIIHQCEVCNKIFPTGQALGGHKRCHWTASADQTDSQPQAQAPLVMSSSGEEASTQTGGTHIQTISIDLNLPPPMEDDLEAQGGGGGGAAEHATRLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZDF4</accession>
    <name>A0A164ZDF4_DAUCS</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0521753</name>
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    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="605" mass="68242" checksum="13A15DB12DE2FAB6" modified="2016-07-06" version="1">MSDRLTRIAIVSSDRCKPKKCRQECKKSCPVVKTGKLCIEVTSAAKIAFISEELCIGCGICVKKCPFEAIQIINLPKDLDKDTTHRYGPNTFKLHRLPVPRPGQVLGLVGTNGIGKSTALKVLAGKLKPNLGRFNNPPDWQEILTYFRGSELQNYFTRILEDNLKAIIKPQYVDHIPKAVQGNVGQVLEQKDERDMKEELCIDLELNQVIDRNVGDLSGGELQRFAIAVVAIQNAEIYMFDEPSSYLDVKQRLKAAQVIRSLLRPNSYVIVVEHDLSVLDYLSDFICCLYGKPGAYGVVTLPFSVREGINIFLAGFVPTENLRFREESLTFKVSETPQESAEEIETYARYKYPTMSKTQGGFKLKVTEGEFTDSQIIVMLGENGTGKTTFIRMLAGLLKPDTVEGSDIEIPEFNVSYKPQKISPKFQHSVRHLLHSKIRESYMHPQFVSDVMKPLLIEQLMDQEVVNLSGGELQRVALCLCLGKPADIYLIDEPSAYLDSEQRIVASKVIKRFILHAKKTAFVVEHDFIMATYLADRVIVYEGQPSIDCVANSPQSLLTGMNLFLSQLDITFRRDPTNFRPRINKLNSAKDREQKNAGSYYYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WNK1</accession>
    <name>A0A165WNK1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415710</name>
    </gene>
    <organism evidence="1 2">
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WNK1"/>
    <dbReference type="Gramene" id="KZM97539">
        <property type="protein sequence ID" value="KZM97539"/>
        <property type="gene ID" value="DCAR_015099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477:SF15">
        <property type="entry name" value="CYSTEINE_HISTIDINE-RICH C1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG96375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="289" mass="31977" checksum="8CABB7BA27314328" modified="2016-07-06" version="1">MSRQTGNCAHSIQDLVQSGDILLRKCNICLDSIFGAGTRFQCNGCSDCFHEICIRPQATLQHSFYPDVIFSFSQGQGTAGQNSQKCVACYKSIQGAVYVSTNDIHIHPFCLVNLTKSVSDDTINFHKKQMVHLLKTSGDRRVAISLDLECQMSLSVGGGRCFICRQKDTIIRNFGWAYKYTYTAQGRSTKFECHVACLRAKLNNNFLNQIVNHHPGSTYDQNTYFLDLNRSKLEKSARDVAGLALPVAVAGLDLMISAFVGSPLLSCLLSCLLEAGADKVEKKLRGSRR</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        </source>
    </evidence>
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</entry>
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                <person name="Ellison S."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="3">Leaf</tissue>
        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="267" mass="31378" checksum="642C4074B8589498" modified="2016-07-06" version="1">MNNLRGAICRSQPLIASSCHRFRCIISQPTIRVSLPSYKPHRVSIFSDVSKGEPWLGFNHQRRTLIQAANWTDGSSPYDILELEGDADDENIKIAYRRLAKFYHPDVYDGRGTLEEGETAETRFIKIQAAYELLMDKEQRRQYDKDNRVNPMKASQAWMEWLIKKRKAFDQRGDMAIAAWAEQQQREMNIRVRRLSRSKIDPDEERRLLAKEKKATLENYNNTLRRHTLVLRKRDLMRKKADEEKKKVISRLLAAEGLELETDEEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A857</accession>
    <name>A0A166A857_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">RIN4 pathogenic type III effector avirulence factor Avr cleavage site domain-containing protein</fullName>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A857"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="102" mass="11751" checksum="E76C6E8B47CABFEC" modified="2016-07-06" version="1">MTNKKAVALPKFGEWDLKNPGTSEFSVIFEKARNAKRDGHPHKFNPNSPFFKKEAEPQWTPAYINKNNASQSQKRPKGRSRRGRPGVRRWFCCSAPTRYAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AC14</accession>
    <name>A0A166AC14_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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        </source>
    </evidence>
    <sequence length="166" mass="18792" checksum="6467ACBA4416D229" modified="2016-07-06" version="1">MALIKLVGLLLMSLLFVSLRGSSANVHLGRKMKSDKLMASKEKYTPEMGIPAQDSLDAHVEEEGDEARKMVNKEDIDNEVESSKKLKSSGAAHNARKTSCNRHHEDKDEHVNSSSSCNMLIHKSRIIGLNEEELEAAEREVMELMRRDYRSKPRRKPPINNNKPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B7E0</accession>
    <name>A0A166B7E0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B7E0"/>
    <dbReference type="Gramene" id="KZN02454">
        <property type="protein sequence ID" value="KZN02454"/>
        <property type="gene ID" value="DCAR_011208"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214754"/>
    <dbReference type="OMA" id="QFFFFLY"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01924">
        <property type="entry name" value="cyclophilin_TLP40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.290">
        <property type="entry name" value="Oxygen-evolving enhancer protein 3 (PsbQ), four-helix up-down bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048563">
        <property type="entry name" value="CYP38_PsbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044665">
        <property type="entry name" value="E_coli_cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023222">
        <property type="entry name" value="PsbQ-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43246">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP38, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21329">
        <property type="entry name" value="CYP38_PsbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101112">
        <property type="entry name" value="Oxygen-evolving enhancer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG93390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="49556" checksum="251AD4C046CC1643" modified="2016-07-06" version="1">MAALIFAQSSAPLRVSTLNSLINPRKSTQFGDHSLAFKASNRTKRPQCSSQIPPQLQLQSKDKDRFFPLKECAISIALTVGLLTAVPALGWPSLASASSNAAFPDLAVLISGPPIKDPGALLRYALPIDNKAVREVQKPLEDITDSLKIAGVKALDSVERNVRQASRAEKQGKSLIISGLAESKKDHGIELLGKLEVGMDELQKIVEDKKRDAVASKQKELLQYVGGVEEDMVDSFPYEVPEEYRSLPLLKGRASVDMKVKVKDNPNIDECVFRIVLDGYNAPVTAGNFVDLVKRHFYDGMEIQRADGFVVQTGDPQGPAEGFIDPSTEKTRTIPLEIMVEGEKAPFYESTLEELGLYKAQTKLPFNAFGTMAMAREEFENNSGSSQVFWLLKESELTPSNANILDGRYAVFGYVTENEDFLADLKVGDVIQSIEVVSGLENLVNPTYKIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BZV1</accession>
    <name>A0A166BZV1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_011855</name>
        <name evidence="17" type="ORF">DCAR_0313388</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BZV1"/>
    <dbReference type="Gramene" id="KZN03099">
        <property type="protein sequence ID" value="KZN03099"/>
        <property type="gene ID" value="DCAR_011855"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211083"/>
    <dbReference type="OMA" id="RFFLFAQ"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000486">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000480">
        <property type="entry name" value="Pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000062">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052451">
        <property type="entry name" value="Ser/Thr_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48008">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48008:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007871427" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="41"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="539"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="486"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="790"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZN03099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG94097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="827" mass="89124" checksum="E74FEC4C8EF8F8D6" modified="2016-07-06" version="1">MKMIQSHQSSISKLSNLVFKLAHFIVLLELLIFTIQPVSSQEWDGVIVTQADYQALQAFKQTLIDPKGFLKSWNDSGFGACSGGWEGIKCAQGQVIVIQLPWRELSGKITDKIAQFQALRKLSLHNNFIGGPIPVGLGFLPNLRGVQLFNNRFSGSIPASLGLCRSLQTLDLANNSLTGNIPASLANSSKLFMLNVSFNSLSGLIPTSLMQSSSLKFLALQNNNLSGPISDSWGEPSGINVSRIQLLTLDHNAFTGSIPASLVQLKEIQVLTLSHNKFSGEIPSEIGGLSKLKTLDFSFNSINGSLPISLSNLSSLTFLNLESNNLDNNITESIGELHNLTVLNLRNNHFVGEIPGSIGKIVSLGQLDVAHNNLTGGIPVSLGDLPNLKSFNVSYNDLSGPVPILLSQKFNSSAFVGNLQLCGYSDSTLCPVSPSPSEEKKKDHGNKLSTKDIILIAAGALLVVLLLLCCILLCCLFRRRTESKGKDSEAATRAVPPPSGDKQVPPTASEVEAGGSGGNGGKLVHFDGPIVFTAEDLLCATAEIMGKSTYGTVYKGTLEDGNQVAVKRLREKLTKNQKEFEKEVNVIGKIRHPNLLAMRAYYMGPKGEKLLVFDYMPKGSLTTFLHARSPDTPINWLTRMKIMQEIAKGLLHLHNNANMIHGNLTSSNVVLDEHTNAKVADFGLSRLMTAAANSNVIATAGALGYRAPELSKIKKANRKTDVYSLGVIILELLTGKSPGEGADLPQWVASIVKEEWTNEVFDLELMKDASAIGDELLNTLKLALHCVDPSPSSRPEVQEVLQQLEEIRSDTTPTSSDEAGAGTSTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C3V0</accession>
    <name>A0A166C3V0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0313582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166C3V0"/>
    <dbReference type="Gramene" id="KZN03250">
        <property type="protein sequence ID" value="KZN03250"/>
        <property type="gene ID" value="DCAR_012006"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213015"/>
    <dbReference type="OMA" id="HNANECI"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000077">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.360.10:FF:000002">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG94289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="464" mass="52283" checksum="E3B59AE701432DA8" modified="2016-07-06" version="1">MSTKNPNSSDLASEPKKRRRVGIAKIDAGVDPADCIKIYLVSSKEEVDAPESFCVESVDFERFFEEDGKIYGYKGLKITIWVSTISFHAYADISFESKSDGGKGITDLKASLQNIFAETLLDEKDKFLKTFSAERDIVKSMVSNAKIVEHKAPNGHSGDSNSDLKSDASSLEVVRVVGTSVGEVYVRLVPLAMLLVDGSSPIDIMDPRWELYLLIQKKVNDQEDTQHRLLGFAALYRFYHYPGSERLRLSQILVLPPFQRKGYGRFLLEGLNVVAVTEDAYDLTIEEPLDSLQHVRTCIDVQRLLAFDPIKDALASVVLSLKQENLSKRSQTVLFGPPLDVVEEVRKSLKITKRQFLQCWEVLIYLGLDPVDKYMENYRTIILDHIKASVIGKDAGDAGKQVVDVPTEFDQELSFVMFKSQNAESSIVAREDDQNNQEEQIQQLVSERIKEVQLIARKVSQQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DAA6</accession>
    <name>A0A166DAA6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_005835</name>
        <name evidence="5" type="ORF">DCAR_0206564</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DAA6"/>
    <dbReference type="STRING" id="79200.A0A166DAA6"/>
    <dbReference type="Gramene" id="KZN04998">
        <property type="protein sequence ID" value="KZN04998"/>
        <property type="gene ID" value="DCAR_005835"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206401"/>
    <dbReference type="OMA" id="SSVHHME"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000046">
        <property type="entry name" value="Rho GTPase-activating protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044785">
        <property type="entry name" value="RopGAP1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177">
        <property type="entry name" value="MKIAA1688 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF35">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="115"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN04998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="384" mass="42895" checksum="09DD850691819D6C" modified="2016-07-06" version="1">MTGMMMKRKLSVCGGGKELRTTNATEEDQDVSVSLVASLVSAIRKSMVSCRFEDADKQDRGLSSSSAMEIGWPTDVQHLTHVTFDRFQGFLGLPAEFQVDVPSKVPSASVSVFGVSAESMQCDYDSRSNSVPTILLLMQERLYTQKGLTAEGIFRINPENSKEEYVRDQLNKGIVPKDIDVHCLAGLIKAWFRELPSGVLDGLFPEEVLQCNTEEESVELVKQLKPTETALLNWAIDLMADVVEHEDSNKMNARNIAMVFAPNMTQMSDPLTALMHAVQVMNLLKTLIIKTLREREETTIEGYSPMSNCSSARHSDEEIDNQHEMNTSCELIGPHSEGNADLHYNDNSESESEIGSFGEIEENFLKQLDDNVNTNSFREFKGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166E4M2</accession>
    <name>A0A166E4M2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_006923</name>
        <name evidence="5" type="ORF">DCAR_0207818</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166E4M2"/>
    <dbReference type="STRING" id="79200.A0A166E4M2"/>
    <dbReference type="Gramene" id="KZN06086">
        <property type="protein sequence ID" value="KZN06086"/>
        <property type="gene ID" value="DCAR_006923"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208338"/>
    <dbReference type="OMA" id="ISATCLC"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF585">
        <property type="entry name" value="OS02G0648100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="50"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN06086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG88583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="348" mass="39339" checksum="96FFE49B4E286C5D" modified="2016-07-06" version="1">MAFLKFLKFKLRQKRVHDAVSDENTHDNILDDFVARRFSWDEIQVITMNCSSSRMIGYGGFSSVYLAKFLDSSLGAVKIQCSSERLDQVYKQELNILLTVSHPNIVRLRGYCDERDQGVLVFDYMCNGTLDEKLRGRGHDSSYLSWKTRMVIAFQLALAINYLHDECSLQIVHGDIKASNVLLDENFNCKLCDFGSAKMGFTSLVKPPSSSSMSRNRMIVGSQGYLDPHYLKTGLASKKQDIYSFGVILLELISGKEAFCAKKRQNLTTIADPMMRDPLKVRDMVDPRFDSDHTFQLEEAKIMASVSGKCLGQSPSTRLSANEIIKIMKDQVPSISYLFQKSATIAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ETE3</accession>
    <name>A0A166ETE3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_007793</name>
        <name evidence="11" type="ORF">DCAR_0208798</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ETE3"/>
    <dbReference type="STRING" id="79200.A0A166ETE3"/>
    <dbReference type="Gramene" id="KZN06956">
        <property type="protein sequence ID" value="KZN06956"/>
        <property type="gene ID" value="DCAR_007793"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206938"/>
    <dbReference type="OMA" id="MHREENA"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF41">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872855" evidence="9">
        <location>
            <begin position="27"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN06956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="356" mass="37949" checksum="970C0E9E341AC2BF" modified="2016-07-06" version="1">MAIPSKLLTLILILLIVISAVPTISGETNTTTPPPECSRPVGGCHNKSHALKLKIVAIFAILITSMIGVGLPIITRAVPALSPDRNLFVIVKAFASGVILATGYMHVLPDSFDALKSECLPEKPWHAYPFTTLVAMFSAVLTLMVDSFAMSFFKKHYSKELEEQKATKIDNENAAELQNTALHSCHSGAVKEFDSTLLRCRVVAQVLELGIIVHSVIIGLSLGASDNPCTIRPLVAALCFHQMFEGMGLGGCILQANYRAKTNAIMVFFFSATTPFGIILGIGLSNVYTDNSPAALIVVGLLDAASAGLLNYMALVDLLAPDFMGAKLQGNIKLQAWSYVAVVLGAGGMSVMAIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FP32</accession>
    <name>A0A166FP32_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_000680</name>
        <name evidence="4" type="ORF">DCAR_0100468</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FP32"/>
    <dbReference type="Gramene" id="KZN08011">
        <property type="protein sequence ID" value="KZN08011"/>
        <property type="gene ID" value="DCAR_000680"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205498"/>
    <dbReference type="OMA" id="FIHEDDH"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF91">
        <property type="entry name" value="PROTEIN ENHANCED DISEASE RESISTANCE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="2">
        <location>
            <begin position="48"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN08011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG81322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="306" mass="33471" checksum="E228148EF15EBB1B" modified="2016-07-06" version="1">MGSTKQKQKHRSSGADQCTKNEANDWKDEVINGGSLRHVDLNNGSNGWASPPGDLFSLRSKTYFAKKVKAPSGPWLLQPAGVDWLRSAGKLENVLSRADNRVMHALKKAQARGKNMKSFVVAVNLQVPGREHHSAVFYFATDEPVPSGSLLHRFINGDDAFRNSRFKIVNRIVKGPWIVKTAVGNYSACLLGKALNCSYHRGENYLEIDVDIGSSAIATAILHLALGYVTSVTIDMGFAIEAQCEEELPEKLFGAVRVLQMEMSSATFVETVSCDKKVNGVGRSSNKVEASRSDEEDENEGLKNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FQL2</accession>
    <name>A0A166FQL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0100523</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FQL2"/>
    <dbReference type="Gramene" id="KZN08062">
        <property type="protein sequence ID" value="KZN08062"/>
        <property type="gene ID" value="DCAR_000731"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201929"/>
    <dbReference type="OMA" id="WLLEVDF"/>
    <dbReference type="OrthoDB" id="47475at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02660">
        <property type="entry name" value="Peptidase_C19D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000089">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF49">
        <property type="entry name" value="UBIQUITIN C-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG81377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="557" mass="63467" checksum="981A4533B175276F" modified="2016-07-06" version="1">MSQRDLLYITPKPCRHLVEYKIKNGITGYNVIQSYIKSGFYSGKVIKNARSEIPRCGFCCGSKGRLYVCLICLEVSCSLFDESNHALLHTQSEYGHEIAVDVERAELYCFSCCDQVYDPDFDKAVMCKYGLGLRNGSNGVVGGELRYSKRKRLDFGVDLFLRNDKKLVWDNDRRSKSCFPLGLRGLNNLGNTCFMNSVLQALLHAPPLRNYFLSDQHNREACRKRSSDKLCLPCDMDVIFSAAFSGDRSPYSPAQFLSSWWQISENLASYEQQDAHEFLISMLDRIHEKEVKTKYPNKDNVNCHCIAHRVFSGLLRSDVTCTTCGFTSTTYDPCMDISLDLNTSSCSNRDLPNKVSKPTECTASSTLGGCLDLFTRPEKLGSDQKLFCQGCQERRESVKQMSIRKLPLVLCLHIKRFEHSFVRKMSKKIDRHLQFPFSLDMTPYLSSSVVRKRFGNRIFAFEGDESDLSTKFDVFAVVTHSGMLESGHYVTYLRLKSHWYKCDDAWITEVDEEVVRSLQTYLVFYVQKTLCTKSVEDVGCLPLSPRGDKFVPISGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FZH7</accession>
    <name>A0A166FZH7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0100869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FZH7"/>
    <dbReference type="Gramene" id="KZN08368">
        <property type="protein sequence ID" value="KZN08368"/>
        <property type="gene ID" value="DCAR_000914"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TF_TCP_subgr"/>
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        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF170">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
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        <location>
            <begin position="189"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG81718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="253" mass="26802" checksum="E7F4F49E98480691" modified="2016-07-06" version="1">MTYSSSSELPRVPGEEHKGAGESEGPLHWLLEHAEESITRAYGGSTKITETDKEAELVHDDGGSKTRGQATVGPRPVFIPGRRYWIVPQGGGPQQVSPVSIYGSYPCEPSQPAPRKDLHTKVEGGGRRTRIPTLCAARIFQLTKELGLATDGETIQWLLQQAEPAIIRATGTGTVPANATVTADGTLGVPDTNKGAAEDVSKTSGLAPGAPSPVSNQNQTSVSCEARVSSAAEEEYDEVVLMGKKIRFRKGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H407</accession>
    <name>A0A166H407_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">DUF2828 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0102436</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG83261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_002348"/>
    </dbReference>
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    <dbReference type="OMA" id="PWKGLVI"/>
    <dbReference type="OrthoDB" id="1149618at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF2828"/>
    </dbReference>
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        <property type="entry name" value="DUF7788"/>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="OS06G0652100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31373:SF27">
        <property type="entry name" value="TROVE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <location>
            <begin position="19"/>
            <end position="417"/>
        </location>
    </feature>
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        <location>
            <begin position="420"/>
            <end position="600"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG83261.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="70518" checksum="B844E83144D50703" modified="2016-07-06" version="1">MDFINALKSGFNQPMMGVTENNSATYLSSGNPCLDFFFHVVPSTPSSQLLQRLEASWSHDPLTTLKLICNLRGIRGTGKSDKEGFYSAALWLHKHHPKTLAGNAHVFASFGYFKDFLEILFRLIQGPDARAILKQEWRAKKNSRGRGIEGVRKDVHFKLDSRNKKEKDIRSAEKKLVRNLISKEVRIEDNKTKMMEDKKRASQLRKEKHAAKAKKAMDMYTNDMNYQFFHESVSTLFADLLKADMEWLNLGKTNNISLAGKWCPTIDSSYDRYTLICARIAKKLFPQEVYSEYEGIGDDQYVLAVRDRLRKQVLVPLHEALKLPEVYMSARKWSTLPYKRVASVAMKTYTDIFMDHDKERFSQYLEDVKQGKAKIAAGALLPHDIIRSCLAGHSDGQEAVAELQWKQMVDDMLKKGQFTNCIAVSDVSGSMVGTPMEVSVALGLLVSELSDDPWKGQIITFSSDPELQMIKGSNLREKCQFVKGMKWGMNTNFQKVFDQILQVALASNLSEEQMIKRVFVFSDMEFNQASWHPWETDYMVIQRKFREKGYEKVPEIVFWNLRESSATPVKATQNGVALLSGFSKNLLTIFFKGGHMNPETVMQAAISGDDYQKLVLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H5B4</accession>
    <name>A0A166H5B4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0102490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KZN09742"/>
        <property type="gene ID" value="DCAR_002398"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226042"/>
    <dbReference type="OMA" id="FFPIHKD"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="40881" checksum="AE2A20C1ABEA2D75" modified="2016-07-06" version="1">MAKKKKRDDSEEHPQSHHQQLENEPSCVNGSNSDEKNDKKKKKKKKHKEDEKSTQIKEKPTVSIALPGSIIHNAQSLELATRLAGQIARAATIFRIEEIVVFDNKVDSGDSSFVLPDSSDENETGSAFLIRILMYLETPQYLRRTLCPWHNSLRFAGLLPPLDAPHHLRKHEWGTYREGVILKERAAESRQSLADVGLNKNVVIDQVLEPGTRVTVAMGTNRHLDDKPRQIVSSSKPREEAGAYWGYKVRYAANISSVFQSCPYQGGYDHLIGTSEHGLFFKSSELTLPPFRHLLIAFGGLAGLEECFEEDKNMKGKKPSDVFHSYLNVCPNQGSRTIRTEEAVFISLQYFQEPIDRALQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H5Q9</accession>
    <name>A0A166H5Q9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102511</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H5Q9"/>
    <dbReference type="Gramene" id="KZN09758">
        <property type="protein sequence ID" value="KZN09758"/>
        <property type="gene ID" value="DCAR_002414"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226286"/>
    <dbReference type="OMA" id="HPFWFAL"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015414">
        <property type="entry name" value="TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12677">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12677:SF24">
        <property type="entry name" value="OS07G0655900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG83336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="259" mass="28408" checksum="C2E5501680ED97C0" modified="2016-07-06" version="1">MAFNWFSFLRYTLVVLFVAATATAFITLPVEKILKDFLIWVDKDLGRWGPLVLAVAYIPLTILAVPASVLTLGGGYLFGLPVGFLADSAGATAGAAAVFLLGRTIARSFVISKVKDYPQFQAVAIAVQKSGFKIMLLLRLVPILPFSMLNYLLCMTPTSTVEYILATWLGMMPLTLVLVYAGTTLKDLSDVTNGWHELSKSRWALIIFSLLVSVVLIFWVTKVAKSALEKALAENEDIHNFSTFIDSPADIRQPLVVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YA27</accession>
    <name>A0A175YA27_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YA27"/>
    <dbReference type="Gramene" id="KZM80399">
        <property type="protein sequence ID" value="KZM80399"/>
        <property type="gene ID" value="DCAR_032378"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192230"/>
    <dbReference type="OMA" id="ATRACSN"/>
    <dbReference type="OrthoDB" id="440385at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG86105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="26324" checksum="4D63CF7AF956B025" modified="2016-09-07" version="1">MANDFKFPPVVFPSGGNPSPGSQRRPATAPFQSPRSSNPGIPFLSFDVGAAATSFPPPQFSSSGSIPGGFEDEPPLLEELGINTKQIWNKTMSILNPFRVKPDLHEDSDLSGPFLFLMAFGLFQLLAGKLHFGIILGWVTVSALFLYVVFNMLAGRNGNLDLYRCLSLIGYCMLPIVILSALSLFVPQGGGVILGVTGVFVIWSTRVCTRLLVELASCGDEHRGLIAYACFLIYVLFSLLVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YG29</accession>
    <name>A0A175YG29_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SirB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_031835</name>
        <name evidence="3" type="ORF">DCAR_0934267</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YG29"/>
    <dbReference type="STRING" id="79200.A0A175YG29"/>
    <dbReference type="Gramene" id="KZM82128">
        <property type="protein sequence ID" value="KZM82128"/>
        <property type="gene ID" value="DCAR_031835"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201750"/>
    <dbReference type="OMA" id="VWVPSPC"/>
    <dbReference type="OrthoDB" id="611769at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF29">
        <property type="entry name" value="PROTEIN SIRB1 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein SirB1 N-terminal" evidence="1">
        <location>
            <begin position="132"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM82128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH14745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="420" mass="47628" checksum="8161464CF3C1BE76" modified="2016-09-07" version="1">MCSKISSSPWILGTSKLSVYENQTRPYFTHTLATKTKSSTSVNPTICGLESGSDDYKFVLHDALDSSGNNTTHARAARDGFCSQVKKLTVVERETSIATNRRVDLARTALYIAAEDDSLISHSSVPLPVEAFLERLDDLSMGYCSHFGSSFQSSPENFLECMDTYLYINKAFCRHSSKNPSEQRTLYLHSVLTHRLGSAAMLSLVYAEILKMFRIWGILNFDVEIFFPHDSSSNPRGYIKQKSKESDQAHIMTTESLLVKILTELKVAFWPFQLDQDKSLFLRAAEAANCSRRSRNVNESASELASVKAAQHRLERGVWTSVRFGDMRRALSACERLILLNTDPKELRDYSVLLYHSGFYEESLHYLKLYQDAEKSSNSTSDLEKNAVDMLLIRLNLILMEDGWSSPPHIRNFLHNSDPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YG92</accession>
    <name>A0A175YG92_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_030263</name>
        <name evidence="7" type="ORF">DCAR_0935151</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YG92"/>
    <dbReference type="Gramene" id="KZM82694">
        <property type="protein sequence ID" value="KZM82694"/>
        <property type="gene ID" value="DCAR_030263"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="97"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="280"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM82694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH15608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YNR2</accession>
    <name>A0A175YNR2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0831800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM84890">
        <property type="protein sequence ID" value="KZM84890"/>
        <property type="gene ID" value="DCAR_027688"/>
    </dbReference>
    <dbReference type="OMA" id="AHHITIL"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215:SF15">
        <property type="entry name" value="DIRIGENT PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46215">
        <property type="entry name" value="DIRIGENT PROTEIN 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620735" description="Dirigent protein" evidence="4">
        <location>
            <begin position="31"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH12298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="372" mass="38225" checksum="06DA4994442EE561" modified="2016-09-07" version="1">MAKLQLHSSNILKTTLYLLLLAITIGCASSARILEEADPQFPVPVNQPVASDPPEIDDPEEAPVVGSSTTLPSGQIPVPAATSPVANVAPIADPAIPLPSVPGPVIPPIATAAPVATTTSPSGPAGTAASGASDTGHPEATLAFFMHDVLGGSHPSGRVVTGIIANSDDNGLPFSKANSQVFPINGGVPLNNINNLVNNNNVPFLAGLNGSPTSTNLHNTGNNNVVSGGNSLPFVSAGQLPAGITLQQLMFGTITVVDNELTEGHELGSSVLGKGQGFYLSSSLDGTSHTLALTTLFHSGDHDHEHVDDSISFFGIHRTGSPISEIAVIGGTGKYQHAKGYATIESLHQVDQHTTDGVETVTHFTVYLTYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W1B7</accession>
    <name>A0AAF0W1B7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0100528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W1B7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053192">
        <property type="entry name" value="Vacuole_Formation_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410:SF216">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="564"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG81381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="608" mass="69587" checksum="BA19218A1C70B7B5" modified="2024-05-29" version="1">MAELKFFFHEHQLILNEAEPVVGKDVECVGCKQPINKLIDAFYRCNKSLIDSSPASDCVGFYMHKTCSELPSTFTHPMNPKKPLSLFVLPYKKEYFYICHACDSHSQHFMYGSDSSVSSEFRVCLKCVMSELKSLEDRNLCHPGHNHPLTLVQSPALFLCHACNTTATDLSYICTRCCFYIHKNCANAPTTYQSKFHNEHALILTYSLPQQYRQYLCYCSICEKHINPIYWVYICANCRFFAHVKCASLTEMLRLIESDIDDEADCGESSLMQFPVHDEASLHETMQQCIIKAVNPSTGEPSPYINHWAHGHQLALGNKNAKTLPLNLKPKIAETELLICDGCMNPISLVDVFYECSLCNFFLHRSCSQFPKRIKHHLAGDLEAVLARESGELYTFQCSGCGIYGNGICMLNDEYTFDIKCASLPRIIKHKGHRHPLQQLKTPDDFLCKGCWREPVTEEVRTIVYGCEKCEFYTHIGCVLSAQVVKHRWDPHPLYLILSLKNVPDHPHEFPCEFCSKEINTNSWFYHCNVCDLSFHISCIDPDDWLSNIKFGATNIYSDKHPHPHGLTYILNKKTRNCNLCGEDARDTPALQCSPCKYIVHRNCFIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W412</accession>
    <name>A0AAF0W412_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0101975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W412"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="159"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="354"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="494"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="529"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="564"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="599"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="634"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="668"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="703"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="773"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="808"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="843"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="878"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="913"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG82807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="116769" checksum="7EA659D63FF66058" modified="2024-05-29" version="1">MKMPLPHCFKHLVCYKNTLTSSIPLRHIIKQQACNPHSSSSLLYHSLSSSKRAKRLYPYLFCTLIHLFLSCRRLSRAIQAFAAMRSQNVVPELASWNRILREFNHAGLVSQVWYVYSEMISCGVFPNVATRNIVIHSLCKIGSISLALDMVRDDYSESDTVSYNTVIWGFCKIGNGKLGLGLCSEMVKKGIPFDSFTCNILVKGFCELGLLRDAEWVVDRMRREGDVVFDVVGFNTLICGYCRAGEVNYALALMKSARNDVCPDIVTYNTLINGLCNMGDFDGAKRLIIEMFKDEEELNTNVDNKVEDFVVRRLGFEPNVVTYTTLIGRYIEQERLDEALSVHEDMISSETLPDVVTYSSVMDGLYKSGRVEDAALLFEEMKRMGVEPNYVTYTVLLDSLFKKGNTLAALKLQSQMLVRGIPFDVVSLTTLMDGLFKVERPSDAENMFKTLLMFNLAPSCATYTALIDARCKVGDVQGAESVLQEMEHNNIFPNVITYTSMICVYLKKGAVDVALYYTRKMDDQNIKPNVFTYGALVDSCLKLGKHDIAIEMYEEMKLKGVKENNHILNAFINNLKRNGQMDEADAFFRNTTSVGLIPDCVSYTSLIDGYFKAGKESAALDIAQEMMERNIEFDVVAYNVLINGLLGLGKYEVQVVYAEMRQRGLIPDCATFNTMITAYCRQGKLESALNLLSEMNEFGVPFNYITCNTLVKGLCEASQVDEAMEVLSKMQGMGLKPSTTAHKIMLDAASKSKRAERIFSVHEQLVGMGLKLNQTVYNTLISVFCRLGMTRIATTVLNDMRAKGFEADIITYNALIRGYCESTQLKLAFTMYYQILDAGVFPNIATYNNLLAGLAAAGLMNKTTELLNDMQGKGFLPNANTYNILVCGHGKIGNKKESIKLYCEMITKGFTPQTSTYNVLISDFAKTGKMKQAGELINEMQLRGVRPSSSTYDILISGYCELSNRVDVGWVSKISYRTEAKRLLEEMIGKGFTPCKTTLSLISHVFAQEGKMVDAQVVLDNLYKRRLVNEQTKNFKDRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5I3</accession>
    <name>A0AAF0W5I3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0101244</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5I3"/>
    <dbReference type="KEGG" id="dcr:108215427"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000276">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000565">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000393">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000457">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g34110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF69">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="788"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="817"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG82083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1086" mass="118230" checksum="9BF66F6121B1201A" modified="2024-05-29" version="1">MEKNKATLDLVSSLFLVYSCFLVVFTRPELVTSLSSDGQALLSLVSTADHYAKSSSSVLSTWTPSSQTPCSWQGITCSPQDRVISVSIPNTFLNLSGLPLELSSLSSLQLLNLSSTNISGHIPSYFGSFSHLHLLDLSSNSLSGSIPPELGQLSSLQFLFLNTNQLTDSIPPQIGNLSSLQVFCVQDNLLNGSIPSQLGSLSSLQQFRIGGNPYLSGAIPLQLGLLTNLTTFGAAATGLSGVIPPTFGNLINLQTLAIYDTEISGRIPPELGLCSELRNLYLHMNKLTGSMPQQLGKLQKLTSLLLWGNSLSGPIPGEISNCSSLVVFDVSANELSGVIPGDLGKLVLLEELHLSDNFLTGSIPWQLSNCTSLTALQLDKNQLSGPIPSQIGNLKFLQSFFLWGNLVSGTIPASFGNCTELYSVDLSKNKLTGSIPEELFGLKKLSKLLLLGNSLSGGLPPSVANCQSLVRLRLGENQLSGHIPKELGQLQNLVFLDLYANHFSGGLPAEIANITVLELFDVHNNHISGDIPSRLGELVNLEQLDLSRNGFTGEIPWSFGNFSYLNKMILNNNLLTGSIPKSIRNLQKLTLLDLSFNSLSGAIPDEIGDVTSLTISLDLSSNGFTGEIPETMSDLTQLQALDLSHNMLHGKIAILGSLTSLTSLNISYNNFSGPIPVTPFFKTLSSDSFLENEDLCQSVNGYTCSSHQFRKNRLKSAKTVALVAVILTSVTIAVVAFWIFVSRNHRYMADKSSCTLASSSGREDFSYPWTFIPFQKLNFTIDNILDCLKDENVIGKGCSGVVYKAEMPNGELIAVKKLWKTKGDEEAAIDSFAAEIQILGHIRHRNIVKLLGYCSNKSVKLLLYNYISNGNLQQLLQSNRNLDWETRYKVAVGAAQGLAYLHHDCVPTILHRDVKCNNILLDSRFETYLADFGLARLMNSPTYHQAMSRVAGSYGYIAPEYGYTMNITEKSDVYSYGVVLLEILSGRSAVESRVGDGSHIVDWVKKKMGSFEPAVTILDSKLRGLPDQMVQEMLQTLGIAMFCVNSSPAERPTMKEVVALLMEVKSTPEEWAKTSQPLIKQSTSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5P6</accession>
    <name>A0AAF0W5P6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0101249</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5P6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="536"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </dbReference>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8N5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="321"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="567"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG84861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="600" mass="66042" checksum="3A3D73E5460A1F3A" modified="2024-05-29" version="1">MNSSMVCSHLMPGSSAISSIVIRSLIFLSTIQMAATLHFKFTNFNHTNDDQIKYERDAFPANNVIHLTNAVIGQSAENDTAYGDGMAFFLVPNGTEVPALATSGGTFGLTNDSQPTNTTANKFVAVEFDVFENSWDPDIDEPEDPLFGRSPDVKIEHVGVDINSVRSNKTVLWDYGRSSVENGWTNEAQISYASNWVSVGFSASTGAAISYNTINSWEFSSTLESHAEAPSPSHRGPVPQVPKHIKTNNKRMGLVIGLAVGGFVLVCAAGIYIILKKKKKERAENENTNCFEDDFMDDDFEKGTGPKKFSYNALAMATSNFAPHGKLGEGGFGEVYKGYLSELNLDVALIGWCHERNNLLLVYEYMQNGSLDSHLFKGKSLLSWKWEQCVVHRDIKSSNVMLDSSFNTKLGDFGLARFVDHERGAQTTMVAGTRGYMAPECFFTGQASRMSDVFSFGVVALEIACGRKPIDLKLEESRVELVKWVWELHGREQILEAADPKLSGDYDESQMKRLMMVGLWCAHPDWPIRPSIKEAIQVLNFDAPLPVLPPQMPVATYSIPSNLSLEDKATTQRSQTHSSGSSNGTDSSVSATAARLNNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9Q3</accession>
    <name>A0AAF0W9Q3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0105088</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9Q3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF38">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041937639" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="27"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="906"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG85895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="953" mass="106299" checksum="07EFCA31227C8BD7" modified="2024-05-29" version="1">MRNSILCFSTISLLLIFLQLQHNLVASSLSPHLQKLALFHFNQTLTISTTSWDCEYYSYDGYYEIPSRAKTKNWSMSSDHCTWEGVSCDDKTGDVIGLDLSCSQLEGAILSNSTLFQLSQLRFLNLSHNGFYNQFPQEFGFFAKGLTHLNLSRTGLSGRVPSGISHLYKLVSLDLGRNYRAWAKLEEEVFKSLLQNLTHLEVLNLEGVSISSVLPLNISTSLRFLALAGTEIHGVVPQEVFHLPNLEFLRLSYNDHLRVTLPKVKWGSSATLQHLQLSGVNIDGGMPDSIGFLESLVSLHLIQCNLSGPIPKFIGQLVQLTHLGLAANNLNLSSLEHNNFTGPFPSFLAHSSKLVELYLHNNSFTGSLPRSLFHHPSLEILNLAYNAFTGPLHEFHSSLSRLRSFYCSSNLLNGSIPHSFSQLVNLTELDLSSNNFTGVLDLEMFSGLKSLGILHLSYNSLSVRTTTMATLPPTLNDLALSSCNMKEFPHFSRDANIFLDFVDLSNNKIEGEIPHWIGSVGRYPFYPIHYLNLSHNGLICGLEQLPWSHVEFLDLQYNKLNGSLPTLMCNSSSLRVLNLSHNKLSGVLPICHTNLTSLRVLDLRRNKIQGNIPATLSNFRNLETVNLNGNRLEGSIPSSFAGFDSLAVLDLGNNQINDTFPQCLEALPNLQVLVLKSNKFYGLINTSSKIVHPFPSLRIIDLSKNEFSGPLPAKYFRNFKAMMDSDVSKIDRSYMNYGSYYSDSTTLVIKGVDMELVKILTIFTTMDVSRNHFEGEIVESIGNLVSLRDLNLSYNHLTGHIPRSIGKLTVLESLDLSSNELEGEIPSQLTGLYSLAVLNLSCNQLSGHIPQGSQFNTFDADSYGGNFGLCGNPLSKTCEQDIVEQEDEEDEDECHFFCGFTWEAVVIGYGCGVVPAFISGYLMLLARKPKWFAGIIGRELGLKIRRMEIRMRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAD3</accession>
    <name>A0AAF0WAD3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0104638</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAD3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22582">
        <property type="entry name" value="BRcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000230">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042159375" description="RBR-type E3 ubiquitin transferase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="95"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="99"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG85449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="417" mass="47737" checksum="6F831D1F28864817" modified="2024-05-29" version="1">MKFVGSNLLFLLLYIQLAVSIKSYVSNVTLYHQITGDSILENPLLLALRQRILEHASTLDFFDLKYVLDIDLTRPLQLAKVAIGVISFPATGDESVDNCSICCEERPSPMMMTIKCSHKFCSHCMKAYVEEKLQSSQVPIKCPQLRCKYNISTRECRSFLPVTSYDIFERAYNFFNEANVLGSDKFYCPYPDCSALLIPHECSLARGSSSNQAGSICLECSVCQRFICVDCGVPWHSSMTCEEYQNLPLEERDTGDVTLHQLAQDKRWRQCQQCLRRIELTHGCYHMTCWCGHEFCYSCGAEYRDSGQTCQCTFWDEEQYPDDVITHPSQEFEQWAWDSFGSLSTMVDAYSEQERSQLALIQRFLAGGLSSGEHQPEQSPPSCTDSYGDSIKDLHQLPWLERFVSVISDNFYDEYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCE9</accession>
    <name>A0AAF0WCE9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0205432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCE9"/>
    <dbReference type="KEGG" id="dcr:108209290"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000020">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF424">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="552"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG86231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="892" mass="102333" checksum="1F8FE628C1BA6447" modified="2024-05-29" version="1">MSSFLDSTAHQRIKRKLDDYDYDFDDDDDDHSPDLIAVRMRKDDSNDAVSSFPSVNSPNHLLNHLIVPNTSCSSSIASSSRGLEVNTRIQFFVRMISGGNTLVFQANCFDTVKSVHERIQGITGIPVIEQRLIYRGKQLQWEKTLAECDVQKDAGLQLVGRMRSTDHPQAWQVIDMMVSTICRMCKGEREVFKKCEGIKRVDVKNQLAEFLTMTPKTCTEQAAGHLQIFISSCAPAAIVMLYLSSLDGNKKCAEDMIGQFISAINMYPKSIHPQCAPILLEFCKLLRRKSQNDGMYKLCRCSLGSMVEYIGIGRSIACKGSEISKALIAFQDIFMFVSELAANLTHDLTVTVESDTVGEQPLLNDVSDFSAFLIPLITVIKEQVILGGLISIPFSEVGYNISCYGEEIKFLYSIFVGLLEKMYLCLGKVEVWLARKEKGEGEILRLGWCHYLAILKELNSISKLYQGAEEAFWTKLRHRKDSICFLITRYAKRSDDHRWILEHKEVTNFECRRHLAMMLLPEVKDEYEELHEMLIDRSQLLAESFEYIARAETETLRAGLFLEFKNEEATGPGVLREWFCLVCQEIFNPQNALFVACPNDRRRFFPNPASKVHPLHLEYFTFSGRVIALALMHKVQVGIVFDRIFFLQLAGMNVTLEDIKDADPFMYSSCKKILEMDPETVDQDALGLTFVREVDELGSIKVVELCHDGRNLSVNSKNRRAYVELLIQHRFVTSISEQVYHFAQGFSDIMNSSEYQKFFFQSLELEDFDWILYGRESTISVDDWKAHTEYNGYKEDDPQILWFWKTIAEMSAERRKVLLFFWTSVKYLPVEGFSGLASRLYIYKSTETHDRLPSSHTCFYRLCFPPYPSLKVLQDRLSVITQEHVGCSFGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WE90</accession>
    <name>A0AAF0WE90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0207577</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WE90"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06632">
        <property type="entry name" value="STKc_MEKK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000186">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF17">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE YODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="149"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG88342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="437" mass="47915" checksum="60AB31459D60AEDE" modified="2024-05-29" version="1">MSSPSPSQVRLCGPELVMNSGACAENPCADLPMLGTGHCSNPGSGHNSVGKNLADQFMIWRHSRGSPECSPIPSPRMITSPGPSPKIQSGAVTPLRPRAGKAIELPAHWPDGKHEGHRLPLPPINYTSYSTETTPVPRSPSRASHGPRWKKGQLLGRGTFGHVYVGFDSENGEMCAMKEVTLFSDDPKSKESAQQLRQEVALLSRLRHPNIVQYLGSEVVDDKLYIYLEYVSGGSIYKLLRDYGQLGEAAIRSYTQQILSGLAYLHARHTIHRDIKGANLLVDPNGRVKLADFGMAKHISGQPCHLSFKGSPYWMAPEIIKNSSSCNVAVDIWSLGCTILEMATTKPPWSQFEGVAAIFKIGHSKELPDMPQNLSDDGKDFIRRCLQRNPLDRPSAAQLLEHPFVKNAAPPPMLVPCLQPLQTMPSVQVRSPVSSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFE2</accession>
    <name>A0AAF0WFE2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Angiotensin-converting enzyme 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0207874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFE2"/>
    <dbReference type="KEGG" id="dcr:108209839"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006476">
        <property type="entry name" value="CHP01589_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01589">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871:SF47">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871">
        <property type="entry name" value="OS02G0137100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09713">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="39658" checksum="0BC13BE1A223A09A" modified="2024-05-29" version="1">MKNDSHNTSEIQHTTHVSHDSQSDQQNNAVEAPVADSGSVSISSNDNRRVSREDIELVQNLIERCLQLYMNREEVVKTLLNRARIDPGFTTLVWQKLEEENADFFRAYYIRLKLKKQIIVFNHLLEHQYNLMKYNPVPPQVPMAPIQNGIHPMPVNNLPMGYPVLQQHPVPATGQPHLDSMGMSTCHVVNGVPAPGNFHPMRMNSGNDMVMDNSTADMTPGIPPGSAMSSMSEMAVSPTSVASSGHFPFTASEIPGMGVDTSALDTAFTSDVASSVGLQLAQDNGAGNSRDSLRSLAQIPWSFSLSDLTADLSNLGDLGALGNYPGSPFLPSDSDILLDSPEQDDIEEFFVDPGPPAQSDEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGP1</accession>
    <name>A0AAF0WGP1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0208014</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGP1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF98">
        <property type="entry name" value="POTASSIUM TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="12">
        <location>
            <begin position="23"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="13">
        <location>
            <begin position="534"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG88779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="777" mass="86992" checksum="82E7C23B706DAACD" modified="2024-05-29" version="1">MDLETGSNKNHVKRESWRTVLTLAYQSLGIVYGDLSTSPLYVFNSTFAEDINHSETNEEIYGVLSFVFWTLTLVPLVKYVFIVLKADDNGEGGTFALYSLLCRHARVSSLPNNQLADEELSSYKTEVGANPDPDRIGSRLKSTLEKHRVLQRILLILALIGACMVIGDGILTPAISVFSAVSGVEQLAMHKEHHKYVEVPVACIILIGLFALQHYGTHRVGFLFAPVVIIWLLTISAIGLYNVLHWNPHVYRALSPYYMYNFLKKTQKRGWLSLGGILLCITGSEAMFADLGHFSQLSIKIAFTTFVYPSLILAYMGQAAYLSQHHMTAHDNDVGFYVSVPEKIRLPVLVIAILAAVVGSQAIITGTFSIINQCSSLGCFPRVKVVHTSTSIHGQIYIPEINWILMILCLAVTVGFRDTRRLGNASGLAVITVMLVTTCLMSVVMVLCWHQSIFYAICFLFFFGTIEALYFTASLVKFLEGAWVPIALALIFMIVMYVWHFGTIQKFEFDLQNKVSVDWLLSLGPSLGIVRVRGIGLIHTELVSGIPAIFSHFVTNLPAFHQVLVFLCVKSVPVPHVEQEERFLLGHVGPREYRIYRCIVRYGYRDVHKDDMEFENDLICSIAEFIRTGKSDTNRDIHSGNNNEKMMVVGTPSTSAQSINMWEDKSEDSEMAGTSEQDIQSPEVKKPKKRVRFVVPESPKIDRVAQDELQELMEARESGVAYILGHSYMKAKPASGFMKKLAINYGYEFLRRNSRASTNALNVPHASTLEVGMGYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHZ0</accession>
    <name>A0AAF0WHZ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3741 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0209488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHZ0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022212">
        <property type="entry name" value="DUF3741"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212">
        <property type="entry name" value="ADHESIN-LIKE PROTEIN, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212:SF4">
        <property type="entry name" value="ADHESIN-LIKE PROTEIN, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12552">
        <property type="entry name" value="DUF3741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="303" mass="34394" checksum="E56F5B5CFFA65360" modified="2024-05-29" version="1">MHKFALEILDSNNELFLKVLKDPNSLLVKRIRNARNLQQAKKETVKLLYENKLVECGNNAISIKSQKPTHHTNKSLLERIKLQYGCPSTDAEVPSTSNSIVLLKPRQTKHERDIFRKHRNLESSNRATGGKKLHLPTVSSFNQRDIEAKKHLSEMLRNAENVEHYTDKQGPRTLKSILSSPLSSPVHEYSTASNQEKGMDASVCPQRIQFSSCSNYEFARDDNSSPLQNSEVAASSTLMKLTGDSPIVRFTKEITEPVYSSDGLSYEGKLAGIHLLKFLLKLLGYRLRLKLCLTVQLMRMTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WI06</accession>
    <name>A0AAF0WI06_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0207948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WI06"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="C2_SRC2/BAP"/>
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    <dbReference type="PANTHER" id="PTHR32246:SF152">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="C2"/>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="36900" checksum="3521B9F7C160E235" modified="2024-05-29" version="1">MSTMPPITIPPNMDETPYKLLEVNVISAHDLPCTAKTLRTYVSARVNDDHNQKTPLDHKGHINPSWNHKMVFRVDEKFMRSRSSAITFEIYNVAWLRDLPIGTTRLPIQNVLNNNSAMKPMALPISRPSGLLKGTINVTINIVDKDVDSEAKQKVKDKSCQSLEEIIAQKFEPSKDLENVQDFKLMLNEKDSKVVKKTRRKRCSSKSTESGTLRPLPSDIVAALNETSRVNSPEHQSFARSATFDNNWMAGVDDESTKEQTKMNSFDWDESDDGKHKKKRPHHQHHNRRHSDGGGLLLCFIKGFEFTFACGGSSRVKKNKAEMVAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJH6</accession>
    <name>A0AAF0WJH6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16122">
        <property type="entry name" value="40S_SA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS2 C-terminal" evidence="8">
        <location>
            <begin position="204"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="303" mass="33186" checksum="438B966127A29821" modified="2024-05-29" version="1">MASGGVARELTSKEADIQKMLAAEVHLGTKNCDFQMERYVFKRRNDGIYIINLGKTWEKLQMAARVIVAIENPQDIIVQSARPYGQRAVLKFAQYTGANAIAGRHTPGTFTNQLQTSFSEPRLLILTDPRTDHQPIKEAALGNIPTIAFCDTDSPMRYVDIGIPANNKGKHSIGCLFWILARMVLQMRGVINQGPKWEVMVDLFFYREPEEAKEEEEEAALADYADYSAPALGGTDQWSSQIPDAQWNNEGVQAAIPAVPGATGWTADAVPAADGWDVAPEPTMAAAPGLDITQPGVVAPTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJP4</accession>
    <name>A0AAF0WJP4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJP4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF24">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="65"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="295" mass="33532" checksum="0B35B09FE0A01CC0" modified="2024-05-29" version="1">MKSAGNAASKLRKGAWDFEEDALLRKCIEKYGEGKWHLVPQRAGLNRCRKSCRLRWLNYLRPTIKRGEFSEDEVDLMMRLHRLLGNRWSLIGGRLPGRTANDVKNYWNTNIQKKLTTGSNQKEVAIREEIVQRKQDGNVAATSTSDRATTVIKPLPRMLSKGTSLPCYNLNLKNRVSFGLNDKTLQNNNNENNKKPSLPAMLPLNDEGNETLTPDEDGTEWWKNLFAEIDIDGQEQDSSQGLLMASSSGLENADADIDLMRKTDKSTTAIMEFSDDLSDIWDLLDPPDYIWLSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLV2</accession>
    <name>A0AAF0WLV2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0209523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10506">
        <property type="entry name" value="RNAP_IV_RPD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040403">
        <property type="entry name" value="NRPD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF36">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE IV SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11523">
        <property type="entry name" value="DUF3223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="7">
        <location>
            <begin position="224"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1440" mass="161249" checksum="4CBE598C6E499EF5" modified="2024-05-29" version="1">MGHDQEEQVVTTGFVTGIKFDILTEKDAEMISVKEIEVPNDVVHPMLGLPNQTSSQCLTCEAEDVKTCEGHFGLINLPYTILHPYLISDVAKILNKICVGCKSERSRAKSADSSSTHKKPTHKKRERCKYCDGLRKDEYPRTIFKVSSKEVFGRTAIFAKINDRVFSRIETLASDYWNFLAEDTQQAGNALKPNERVLSHLQVHELLKEVDPKIVKEFVKDKKDLIFMNNFPVTPNSHRVNEFGGQVRFDELTRCYRKLANFEGSANELSTRVLDCFKMCKLRSEKVSTKMFNQNEEAAPNLSGLRFIKELILGKRSDHTFRMVVVGDPRIKVDEIGVPSHIADSMQISEQLNSLNWESLGLKILRKRDTYIRRDGRLARVTIKDDKLRIGDTVHRPLRDRDIVLINRPPSIHSHSLIALSVKILPTNSILSLNPLICSPFRGDFDGDCYHGFIPQSVDSRVELQELVALNKQLTNIQNGLNLISLTQDSLTAAQLVIEDGVLLNRSQMQQLQMFCPRSCQLQAPAIVKASSRKNCLKAASSDTFLWTGKQLFSLLLPQDFSYDCPSSAVKIMKGELISSKTASSWLRDTKGNLLYSLISHCQGEFLDFLHAAQGVLSEWLFMRGLSVSLADMYLSSDSHCRKNMIDEVSCGLREAELISSVKLLMVDSNLDFLVGSLEESETAMDFGAKHMFCDRQKSPALIQVSVSAFKQVFSKIQNLFYQYASEDNSFLAMIKAGSKKSNLTKAVQHAMCLGLQHSLVSLSFRIPQELSCEAWNSQKEEESIDPKDDHSKISKSYIPYAVVQSSFLSGLNPLESFVHSLTTRDASFGGHADISGTLTRRIMFFMRDLCMGYDGTVRSAYGNQVVQFSYYNNPTSSKNSPEEKLTDGACTMGGQPVGSWAACAISEAAYSALDLPISALEASPLLNLKEILECGNNKVNKTASLFFSKKLERWTYGFEYAALEVKSRLERLQFLDIVSTVMIMYSPCREAYVSPWVCQFHINKELAKRKQVKVQSIVKALYMKCNSTLVKSKVDLPDLQITCNDSSEKNMATICINIRMVGIDENSSTQLETLEDMVLPFLLQTVIKGSSNVKKVDILWKDSSSLSTSHKGASGELYIRVHMSATCDRSRFWTVLMNDCLQIMDMIDWKRSYPDDIQAATSALGIDFAKSQFINKLASAISDTGKAVLPEHLYLVADCLSCTGEFVALSANGLARQRKQTSVSSPFTQACFSSPADCFVNAGKMGMMDNLQGSLDSLAWGNPPSLGTGAQFDILYSRKDHEVAKPEDIYNLLGSQVGARGQRVKVSRTHTDMSIKNLAKRSDLLRKDFKAKYFLSMSEELRLILHKYKINQQLSELDESIVVAALFFHPRSSEKIGSGLKEIKIGGSSKSSTSRCFHVVRTDGTIEDFSYHKCIHNGLKLIAPKSAETYRSRWLNGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WSF6</accession>
    <name>A0AAF0WSF6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0314199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WSF6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000005">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF714">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X798"/>
    <dbReference type="KEGG" id="dcr:108222391"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000257">
        <property type="entry name" value="Zinc-finger homeodomain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006455">
        <property type="entry name" value="Homeodomain_ZF_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01565">
        <property type="entry name" value="homeo_ZF_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF140">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
        <property type="entry name" value="ZF-HD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="11">
        <location>
            <begin position="37"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH02688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="197" mass="22547" checksum="75829F3D3CFC0F17" modified="2024-05-29" version="1">MEFEEQEQDEEMQQLAPSNYVSTVPEAFRSRAGGYKYKECLKNQAIGIGGNAVDGCREFMPAGQEGTLDALRCAACTCHRNFHRRQSEAETMPPQYHRPSGYLVTPMSQQRPVAAQLEMDQELGNPNPSSSGGGSSRKRFRTKFTREQKERMTEVAERLGWRVQQQDEAVVEEFCKESGIQRQVFKVWMHNNKHTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7J3</accession>
    <name>A0AAF0X7J3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Monocopper oxidase-like protein SKU5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0521308</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X7J3"/>
    <dbReference type="KEGG" id="dcr:108223893"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000012">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000016">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000023">
        <property type="entry name" value="Monocopper oxidase-like protein SKU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF58">
        <property type="entry name" value="SKU5 SIMILAR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="39"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="165"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="402"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH01922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="66812" checksum="5DA454F084D0E250" modified="2024-05-29" version="1">MWVFGGLPDSWFLLYLNLVAGLVISAFGGDPYVFYDWDVSYSTASPLGVKQKVIAIDGNFPGPILEVTTNWNVVVNVKNNLDEPLLLTWNGIQHRRNSWQDGVPGTNCPIPSGWNWTYVFQVKDQIGSFFYFPSLNFQRAVGGYGGIIINNRDVIPVPFAPPDGDITLLISDWYIKSHKDRRKDIENGIGLGAPDGILFNGRGPYRYDTSLVPDSIDYLTLNVEPGKTYRLRVHNVGISTSVNFRIQNHNLLLVETEGSYTVQQNYTNMDIHVGQSYSFLITMDQNASSDYYIVASSRFVNSSAWARAKGVAILHYSNSQGPASGPLPDPPNEYDTFFSMNQAKSIRWNVSAGAARPNPQGSFRYGDITVTDFYVILNRPPELIDGRYRTTLNGISHLAPQTPLKLAQQFKIPGVFKLDFPNRLMNRPAKIDTSLINGTYRGFMEIILQNNDTTVQSYHLDGYAFFVVGMDYGVWTDNSRGIYNKWDGVARSTTQVFPGAWTAILVSLDNVGIWNLRAENLDSWYLGQEVYISVVNPEITEKTELPLPDNVIYCGLLSSLQKDQAQRVKFSDAPPTLKGMRIIWAASVILFSVFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDU1</accession>
    <name>A0AAF0XDU1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0625648</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:dUDP biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH06225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="24342" checksum="1FD299D742F09729" modified="2024-05-29" version="1">MEDKSKSGSRGALVVIEGLDRCGKTSQSSRLVKNLDELGYPAELFRFPDRNTVIGQMISSYLNSDSQLDDRTIHLLFSANRWEKRSLMESKLQSGITLIVDRYAYSGVAFSSAKGLDIEWCKAPDMGLLAPDLVLYLDISPEKASERGGYGGERYEQLEFQKKVAQSYQVLQDASWKIIDANRTVEDVEEHMRDIVLDYVKTCRNGKSLSQLWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF86</accession>
    <name>A0AAF0XF86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0626437</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF86"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="163"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH07008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="340" mass="38145" checksum="17D9B0668D65D9C1" modified="2024-05-29" version="1">MEYTGHDFLEELLSFRNEPWESCTNFIPTGADHGIYNGYGYSNVASFPENPLPNIPQTPCFDELSAYTVPFDEYQQTLNSAALFSEISGPFGDEHTDSSNNMFDTPPFFPCQEEYSMRSIMDELEGKAAADACKMEPVQCTQVPGFSVGSGVERKSKTKKVDGQPSKNLMAERRRRKRLNDRLSMLRSVVPKISKMDRTSILGDTIDYMNELLERIRKLQEENGFGSNDHQGELSLTSIINDVKPNEALVRNSPKFNVERRNMDTQIEMCCANKPGLLLSTVTTLEALGLEIQQCVVSCFSDFGMHASCSEPEEEPTRNLSSEDIKQALFRNAGYGGRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFD5</accession>
    <name>A0AAF0XFD5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFD5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_bL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052010">
        <property type="entry name" value="Ribosomal_LSU_bL36"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10858" checksum="D8A7E63D0064B536" modified="2024-05-29" version="1">MKVRSSVKKMCEFCRAVKQRGRVYVLCTANPKHKQRQGMSTFAFEGPLPPTFFKQEASVDHRLIAGLPSISQKTEPSTMSGWRAGLASLLFGQNKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKU6</accession>
    <name>A0AAF0XKU6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0729343</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKU6"/>
    <dbReference type="KEGG" id="dcr:108195962"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010072">
        <property type="term" value="P:primary shoot apical meristem specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000558">
        <property type="entry name" value="Topless-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000479">
        <property type="entry name" value="Topless-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027728">
        <property type="entry name" value="Topless_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048419">
        <property type="entry name" value="Topless_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054532">
        <property type="entry name" value="TPL_SMU1_LisH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054080">
        <property type="entry name" value="TPR1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44083:SF45">
        <property type="entry name" value="TOPLESS-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44083">
        <property type="entry name" value="TOPLESS-RELATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17814">
        <property type="entry name" value="LisH_TPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21889">
        <property type="entry name" value="TPR1-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21359">
        <property type="entry name" value="zf_topless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="6">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="456"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="919"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1101"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1114"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH09883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1134" mass="124763" checksum="46EC133DCE5219E3" modified="2024-05-29" version="1">MSSLSRELVFLILQFLDEEKFKETVHKLEQESSFYFNMKYFEEEVHNGNWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKNDRPKAVEVLVKDLKVFASFNEDLFKEITQLLTLENFRENEQLSKYGDTKSARAIMLVELKKLIEANPLFRDKLQFPNLKNSRLRTLINQSLNWQHQLCKNPRPNPDIKTLFVDHSCAQPNGACAPSPANNPLLGSLPKVGGFPPLGAHGPFQTTAAPAPTPLAGWMSNTPTVNHLPVSSGAIGFGGPSMPAALKHPRTPPANPSLDYPSGDSDHVTKRTRPMGICDEVNLPINVLPMSFPGHGPSQAFNSPDDLPKNVARTLNQGSSPMSMDFHPTKQTLLLVGTNVGDIGLWETGSKERLVLKNFVVWNVTACSTTLQAAIVKDPGVSVNRVIWSPDGSLFGIAYSRHIVQMYSYHGDDDIRQHLEIDAHIGGVNDLAFSHPNKQLCVITCGDDKTIKVWDANTGAKQYTFEGHEAPVYSVCPHFKENIQFIFSTALDGKIKAWLYDNLGSRVDYDAPGRWCTTMAYSADGTRLFSCGTSKDGESHIVEWNESEGAVKRTYLGFRKRSLGVVQFDTTKNRYLAAGDEFSIKFWDMDNVQILTSIDGDGGLPASPRIRFNKDGSLLAVSTNENGIKILANSDGLRLLRTFENKSYDASRAPESVKPIISVAAAAAAAAASSSGPADRVVSAVSLSGMNGDARNMGDIKPRIADDSSDKSKVWKLTEVSEPSQCRSLKLPENMRVPKISRLIYTNSGNAILALASNAIHLLWKWQRNDRNSSGKATANVSPQLWQPSSGIQMTNEVTDANPEDAVSCFALSKNDSYVMSASGGKISLFNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMDDSSIQIYNVRVDEVKSKLKGHSKRITGLAFSHVLSVLVSSGADAQLCVWSSDGWERQKNRYLPIPAGRTPTAQSDTRVQFHQDQIHFLVVHETQLAIYETTKLECLKQWVPRESAAPISHATFSCDSQLVYASFLDASVCIFSAAHLHLRCRISPLAYLPHSVSNSNVHPVVIAAHPQEPNQFALGLSDGGVHVFEPLESEGKWGVPPPVENGSASTVPATSVGASGSDQAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNB7</accession>
    <name>A0AAF0XNB7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0729662</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNB7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000002">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] iron-sulfur protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="172" mass="19327" checksum="EF3A3F3B5503AC89" modified="2024-05-29" version="1">MASSIHRIVSHTRRAHQTSVLQQLSRHYSTSESLVEYKAGEIGTVSGIPDEHLVRKVVIYSPARTASQQGSGKTGKWKINFVSTQKWENPLMGWTSSGDPYSHVGESALNFDSEDAAKSFAERHGWEYQASSLAFYFMDIGAAYIVKKRQTPLLKIKTYAENFKWKGLPEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPH6</accession>
    <name>A0AAF0XPH6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0730285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPH6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016965">
        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889:SF12">
        <property type="entry name" value="LP01149P"/>
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    </dbReference>
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        <property type="entry name" value="PHOSPHATASE, ORPHAN 1, 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="28160" checksum="1C71B61660DBB079" modified="2024-05-29" version="1">MAGIVVIFDFDKTIIDIDSDNWVIDGLGGTALFERLLTTMPWNSAMDAVMKEFHSQGKTIEDIVAILKQVPIHPRVVPAIKAAHSLGQYFSEINTNPGFVDEEGKLRILPYVDFLKAPHGCSNVCPPNMCKGMIIERIQSSLEKNEKIIYLGDGAGDYCPMLKLGPENFVMPRKNFPVWSLISENRDLVKAKIHEWTDGQELEQILLGLIASISEEEKRSISSAQLFSSDCGFQTIAAMHGHEALPRALPVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPS1</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0730174</name>
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    <organism evidence="1 2">
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPS1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34563">
        <property type="entry name" value="BNACNNG33880D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34563:SF9">
        <property type="entry name" value="MADS-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH10704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="63" mass="7004" checksum="8B5AFD356BCFA2F2" modified="2024-05-29" version="1">MGLENLIGSLKKKVLKGLKKKTGVSDYDKIEKSDSMRVEIQSRKARKLIQNTLKVADSPPCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQT7</accession>
    <name>A0AAF0XQT7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0830900</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQT7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000042">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000106">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000290">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="99"/>
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        <location>
            <begin position="394"/>
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            <begin position="1"/>
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        </location>
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        <location>
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        <ligand>
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        </source>
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        </source>
    </evidence>
    <sequence length="497" mass="57658" checksum="51A90A15BF134327" modified="2024-05-29" version="1">MERRKGETGDSDTKEFEENGEVAKETLERVAAAKKYIESHYKSHMKHIQDRRQRRALLEMKLNSSDVSEEERQNLLKELERTETEYMRIKRQKFCVDDFELLTIIGRGAFGEVRVCREKKSGNIYAMKKLKKSEMLSRGQVEHVKSERNLLAEVNSQFIVKLYYSFQDADYLYLILEYLPGGDMMNLLIKEETLTETVARFYIAQTVLAIESIHKHNYIHRDIKPDNLLLDKDGHMKLSDFGLCKPLDCTNLTTISENESLVSKSNSDSAKKSFWKSPREKLQHWQLNRRTLAFSTVGTPDYIAPEVLLKKGYSLECDWWSLGAIMYEMLVGYPPFYTDDPMSTCRKIVNWRSTLKFPDDARLTPEAKDLICKLLCSVEHRLGADQIKAHPWFKDTVWDKLYEMEAAFKPEVNGELDTQNFYQFDEEIEDTTPTRTGSGRFSKMKDLNFVGYTYKNFEAVKGLRRSGRSTSPDRQSTDSPLAYNSRINLVILCIGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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    </reference>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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        <location>
            <begin position="60"/>
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        </location>
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        <location>
            <begin position="88"/>
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        </location>
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        <location>
            <begin position="923"/>
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        </source>
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        </source>
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    <sequence length="954" mass="108299" checksum="4E7BCE735D145739" modified="2024-05-29" version="1">MSNVKDIHGMARDSVKYIPLHEEYMATAPLVNSNGASKKRKIPERNIDKKDVGDVISKQWSWYGFLCIYLLGWFFLKLCHTLGICRDFIHIHICVMNYLILWAPVYVLTIDGEPDRFIGLECKVYWPLDDDWYCGRILGYNLSTERYHVKYNDGVEEHLCLSFERILFYVSPEEIQQRKLCCVQHPDVDSHDIDNMVSLAASLNHCSRVDPIPGDMIWAKVTGHPMWPAIVLDESACSNLKILNKISGERSVLVQFFGTHDFARLSKKQVTSFIKGLLSELHRKSKTSDFLRSLIEAKTYLSTEKLPSRMLSLQSSNAANFESASGEEDCTRDEEIPKNVIFRSCPFDIGGLQIISLGKIVKDSELLYDRKYICPEGYTAVRKLPSISDPKKCISYTMEVLRDGNTLNKPLFRVTSEKGEQFEGPTSSSCWDKLYRKIRKLHGHDALKAFGEDNKIVRSGPAMFGFSHPKIKELIRLSSTCGSPLDSSKLTSRRCRDPAIGYRRIHITWKDLDKCNVCYTDEEYDISPFLQCDNCRMMVHTSCYGELEPADGTLWYCNLCRVGAPESPPPCCLCPLAGGAMKPTTDGRWAHLACAISIPETCLLDVRKMEPIDGLNRINKIRWKLLCSICGVSHGACIQCSNSSCYVAYHPLCARAAGYFLEFYEEDGLRLCSEKEDEKDQCIRLRSYCKRHRRPSDKCIVVQESMGTCQRPDYIPSPNPSGCARTEPYNYLGRIGRKQPQSHEPASLKHLYLENVPYLVGGIRQHEFLSKVVSSQCNISSNISSMVEKYSHMKETFRRRLTFGKSGIHGYGIFAKQPHRAGDMVIEYTGEVVRVNVADRREHLTYDSLVGAGTCMFKIDDDRVIDATKAGNIARLINHSCEPNCYSRIITVNGDQHVIIYAKRDLNQWEELTINYRFSSVDEQLACSCGVSRCRGIVNDIKAVEQVAKPCIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUC6</accession>
    <name>A0AAF0XUC6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
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    <gene>
        <name evidence="5" type="ORF">DCAR_0832076</name>
    </gene>
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        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="103" mass="11633" checksum="0E648E59833A3C98" modified="2024-05-29" version="1">MSRRLTVQSDVYSFGIVLLELITGRKAIDSTRPQGQQSLVEWARPFIDHRKFAEIADPRLQDQYPMRGLYQAVAVACMCIQEQASARPLIQNVVAALSRLTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUE2</accession>
    <name>A0AAF0XUE2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="10">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0933844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUE2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
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        </lineage>
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                <person name="Bowman M."/>
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                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="8 9"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AN95"/>
    <dbReference type="KEGG" id="dcr:108208914"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF3">
        <property type="entry name" value="LONG CHAIN ACYL-COA SYNTHETASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="10">
        <location>
            <begin position="49"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG86371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="655" mass="72945" checksum="3C16168B56FC8533" modified="2024-05-29" version="1">MANIKRYIVQVEPAKPSEDGKPAMGPVYRSTFGVEPHIPGMESCWDIFRMSVEKYPDNPMLGHREIVDGKHGDYVWLTYKQVYDMVMKVGNAIRSCGVEKGGRCGIYGANSEGWIISMEACNAQGLYCVPLYDTLGASAVEFVICHAEVSIVFSEEKKITEVFKTFPKTAEYVKTIVSFGKVTSEQREEAAKLGVAIYSWDEFLVLGDGKHYELPVKKKSDICTIMYTSGTTGDPKGVLISNNSIVTIVAAVNRFLQGVNEPLTVNDVYLSYLPLAHIFDRIIEECFISHGAQIGFWRGDVKLLVEDIGVLKPTVLCAVPRVLDRIYSGLTQKISSGGFLKNILFNVAYSYKLLGMNNGHKHEEAAPLFDKFVFDKVKQGLGGKVRLILSGAAPLANHVESYLKVVTCSHVLQGYGLTETCAGSFVSIPNEQTMLGTVGPPVPNVDARLESVPEMGYDALSSTPRGEICIRGETLFSGYYKREDLTKEVLVDGWFHTGDIGEWQPNGAMKIVDRKKNIFKLAQGEYVAVENLENIYGLVAAIDSIWVYGNSFESYLVAVVNPNMQAIESWSNENGLSGDFHSLCQTEKVKEYILGELSKIGKEKKLKGFEIIRAVHLDPVPFDMDRDLITPTFKKKRPQMLKYYQNIVDSMYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANA7</accession>
    <name>A0AAF1ANA7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0205512</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANA7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051032">
        <property type="entry name" value="AP2/ERF_TF_ERF_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF300">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="133"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG86310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="236" mass="25706" checksum="C67E396E6DAB5F18" modified="2024-05-29" version="1">MKEQSSSSTSQDRTKCTPSSSTSTSSLKPVVSRKLTKKSAENASIKMVKKSEKCSSSSSSSIADGKHPIYRGVRIRSWGKWVSEIREPRKKSRIWLGTFATPEMAARAHDVAAIAIKGHSAFLNFPDLAHKLPEPASKSPKDIQAAAAQAAAMDDTTTSHETETEAELSGAETRPSEAFGESSSTSPTRRGEDPFLGLPHLFQPNLGPKFGYEFPWSLALAETIDGEIWLDEPLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1APF1</accession>
    <name>A0AAF1APF1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0309888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1APF1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF300">
        <property type="entry name" value="EXOSTOSIN GT47 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042017150" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="131"/>
            <end position="412"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="461" mass="52933" checksum="10D849B0AD28B423" modified="2024-05-29" version="1">MWDFHRRRLTTILILLPLVLISVIACKFSFSSPLASGKKSSVSSFFDSGKYQSEKPLNLVEGVDDFHAQNPASSPNSVKKIEARLVKVRQAIKEAVPNRNMTSLHEDPDYVPRGPMYRNANAFHRSYLEMEKLFKIYVYKEGDRPLFHNGICKSIYSTEGRLISELEKGTLYKTEDPDEALVYFLPFSVVMLVEYLHIPGAREIHAIGNTVADYIKVISQKHRFWNRSQGADHFMASCHDWAPRTTSYVPNLFNNAIRVLCNANTSEGFNPSKDVSLPEINLLTGEITGLVGGPSPSKRPILAFFAGRLHGHIRYLLLEQWKEKDSDVQVYESLPKGVSYQTMLRRSRFCLCPSGYEVASPRVVEAIYSECIPVLISDGYIPPLSDVLNWKTFSVQVDVKDIANIKTILMSISQRQYLRMHRRIKQVQRHFVMNPSPKRFDFFHMMIHSIWLRRLNVRIED</sequence>
</entry>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOG88592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="34607" checksum="A546BA24C89D9CE6" modified="2024-05-29" version="1">MIRFELSDSATTFDDQINEELEYRIGMMETSLSRFGMILDSIQSDIMQVNKGRKELALEMESIRQKLNASDNTLQLMNKGQDDVKVNLDRAVNTICDQLGQYGYQESSHDMISLLTALPEKIELCTQKLKNDICKSFSKEMQGMACSQQILNQKLISAVHLSKVMFYIGPCITQISSFYFLFHFYVSSVQASQVKVQKDSAAVKIEMGTWKSVKSKQATFTDRNSVKAQKQRGTSPTEVERVSRINIESDEEMSRDFFYFLEDKETGTGNFSMQDAREESERILRKARRRKRKHSNTIIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUN0</accession>
    <name>A0AAF1AUN0_DAUCS</name>
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        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0415343</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000042">
        <property type="entry name" value="Envelope ADP,ATP carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="79"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="179"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="269"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG96013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="381" mass="41353" checksum="77A8E70EB7BCC34B" modified="2024-05-29" version="1">MASPNPSKPDGPTFTWHRIPGLDSSPCAPTSACNSGAKLQLSSAGFACVSLVEKREDREFAPSMAQLLKYPLAMFALVPREAALFAAGAVAGAAAKSCTAPLDRVKLLMQTHGLRAGGEGARNSFGFLEALAVIGKEDGLRGYWKGNLPQVIRVIPYSAVQLFAYETYKKLFEGEDGELSVIGRLAAGACAGMTSTFVTYPLDVLRLRLAVEPGRRTMSEVTLNMLRDEGLAAFYKGLGPSLIGIAPYIAVNFCVFDLVKKSLPEKYQKKTEASLVTALVSATIATLMCYPLDTVRRQMQLKNAPYKTFFDAFPGIVERDGLAGLYRGFLPNALKTLPNSSIRLTTFDTVKRLIATGEKELQRIVEDNQKKQKQSATDYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWR0</accession>
    <name>A0AAF1AWR0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108211589"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34109:SF1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="24"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="162" mass="16732" checksum="0F31F6486DB1A7A0" modified="2024-05-29" version="1">MAPEVAQNGAAHNGAVTKTATFSAMKPQLFVEASKANDAVEFYKAAFGAVEISRVTHPKRKADQELPLVISAQLKLGDSVFIVSDDSEAPLKSGTGSVFCLESEEIDVAVEKAVKAGGVSVGEVTEGEGACCGGRVGTVKDPYGNIWMICSPAKKAGADVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYG0</accession>
    <name>A0AAF1AYG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0417083</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG97742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYG0"/>
    <dbReference type="KEGG" id="dcr:108219351"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF1">
        <property type="entry name" value="GH21943P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
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            <begin position="332"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
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        <location>
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            <end position="417"/>
        </location>
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        <location>
            <begin position="6"/>
            <end position="426"/>
        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOG97742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="446" mass="47876" checksum="09FA526C1979C4AE" modified="2024-05-29" version="1">MEKLTPLSHLFMTVFLYSFSTFMVVPAMTDVIMSAVCPGEDECSVAIYLTGVLHAVVGLGTLVMMPLIGNLSDTYGRKVMLTAPMVLAIFPSAILAYSRSPYFVYAYLVIKTLTSMLCEGSVFCIALAYVADNTAEKRRGSAFGILSGVTSCAFVCGNLFTRFISTSSAFQVSAAGTVIAVVYMRIFLPESVAGVGIAGTGDKTEDESLLVKDSSENLQLFKNMPSFRDTISLLKTSPTFSQAAAVAFFTTLGEVGLYTSMLYFLKAQFHYSKDQFADLMVINGIAGTISQMVLMPLVAPFIKEKKILAIGLFFSCVHMLLYSIAWSSWVPYMSAMVTVLAAIGSPCVRSIASKQVGPSEQGKAQGCLTGICSIANVISPLAFSPLTALFLSESAPFHFPGFSLMCASFLVMIAFILSIMMREAPVAETSTDDNPAPSSDLNECTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B030</accession>
    <name>A0AAF1B030_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3 11">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="11">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0418060</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="11"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218305"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001066">
        <property type="entry name" value="DNA helicase INO80-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000006">
        <property type="entry name" value="Putative DNA helicase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBINO" evidence="16">
        <location>
            <begin position="345"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="584"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="1194"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1374"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="417"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1399"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG98715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1499" mass="170253" checksum="42D43D9F96F898F6" modified="2024-05-29" version="1">MDAKRQSRNSIAYSNLFNLEPLMNFQMPRQDDDFDYYGNSSDDDSRGGAMTERTNGKASANKKRRSAYSSDEDGNYGTYISEEQYRTMLGEHVQRYKRRRNNSSPNPGPTRTVMPVLKGSLGPKERKSGNESRVGSRKMESNSEYLADMINQKPGKYNEREFASEYKINRTVYEPAYLDIGESIAYRIPQTYEKLAASLKLPTTSEIRVDEFYLKGTLDLGSLAAMMAADKRAGQRSRAGIGESKPQYESLQARLKSQAANNSIPKFSLKVKDVSLDASSIPEGAAGGIRRSIMSEGGHLQVYYVKVLEKGDTYEIIERSLPQKEKVKKDASMIEKEEAEKIGKYWVNIVRKDIPKHHRIFTNFHRKQLTDAKRFSEICQREVKMKVSRSLKLMRGAAIRTRKLARDMLIFWKRVDKEMAEVRRREEKEAAEALKREQELREAKRQQQRLNFLLSQTELYSHFMQNKATSQASEIPNDQEALMNSSEAAPVAVEEEDPIEAELKLEALKAAQDAVSKQKKITSAFDDDCLKLRQAAPEEAPIAGSSNIDLLHPSTMPVASSVQTPELFKGSLKGYQLKGLQWLVNCYEQGLNGILADEMGLGKTIQAMAFLAHLAEEKNIWGPFLVVAPASVLNNWADEISRFCPDLKTLPYWGGPQERAIIRKNINPKRLYRKDAGFHILITNYQMLVSDEKHLRSIKWQYMVLDEAQAIKSSQSIRWKTLLSFKCRNRLLLTGTPIQNNMAELWALLHFIMPTLFDSHEQFNEWFSKGIENHAEHGGTLNEHQLNRLHAILKPFMLRRIKKDVMTELTGKTEITVHCKLSSRQQAFYQAIKNNISLAELFDSNHGRLNEKRILNLMNIVIQLRKVCNHPELFERNEGNTYLYFGDIPNSLVPAPFGELEDVYYSGSRSPITYKIPKMVYREFIRSSGAFSSAARHVESREFIEKHFNIFSPVNVFRSMFAQGNNLNAAFAGSGTFGFAHLIDLSATEIAFLATGSFMERVLFTIMNWDRKYLDGMLDMFMEAESDDSICSQIGGDKVRAVTRMLLLPSKAETTLLSRRLATGPWDVPNEALVLSHQDRLLSNIRLLHSAYSFIPTTRAPPISAHCSDRNFAYKMLEELHNPWLKRLLVGFARTSDYNGPRKPTAHHLIQEIDSQLPVTQPALQLTYNIFGSCPPMQSFDPAKMLTDSGKLQTLDILLKRLRAGNHRVLLFAQMTKMLNILEDYMNYRKYRYLRLDGSSTIMDRRDMVRDFQLRSDIFVFLLSTRAGGLGINLTAADTVIFYESDWNPTLDLQAMDRAHRLGQTKDVTVYRLICKETVEEKILQRASQKSTVQQLVMTGGHVQGDLLAPEDVLSLLIDDAQLERKIREIPLQARDRQKKKSGTRGIRIDAEGDASLEDLTNSESQVNGSQPSQDPEKEKSTKRKSTSDKPVPPKPKSKKGGSRNSDTSSPALIPLENEGDDPLQNSDSQHRSKRPKRPTKSVNENLEPAFVASPSLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B093</accession>
    <name>A0AAF1B093_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0518484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B093"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050942">
        <property type="entry name" value="F-box_BR-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259:SF108">
        <property type="entry name" value="F-BOX PROTEIN SKIP23-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259">
        <property type="entry name" value="OS07G0183000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="24582" checksum="4C7B8945A58480F0" modified="2024-05-29" version="1">MAVYGELSYLGFARLGDSVWTDVHVPCRNFQDIAYHNGNFYAVNFNGDVYVCSIDDDNKEGHRGMKVASIETYDCDQKYLVEPLSGSGLLVLVRYRQTRHFIEPCGIVAKYRTTHFAVWRLDLTNYDSLENPSYTLTEENSLGNEAIIVGRAASLSVPSSETIRPNSIYFTDDNRECYLNRGGGHDMGIFDMERRTIEPHFQGNSLHCISPPLWYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B175</accession>
    <name>A0AAF1B175_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroflavonol 4-reductase</fullName>
            <ecNumber evidence="8">1.1.1.219</ecNumber>
            <ecNumber evidence="7">1.1.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydrokaempferol 4-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0520102</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="8"/>
    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B175"/>
    <dbReference type="KEGG" id="dcr:108222765"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
        <property type="term" value="F:flavanone 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009718">
        <property type="term" value="P:anthocyanin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF713">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="14">
        <location>
            <begin position="7"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH00728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="347" mass="39163" checksum="09A67E470E448041" modified="2024-05-29" version="1">MEEKRTVCVTGASGYIGSWLVKRLLERGYHVRATVRDPGDEKKVKTLLELPKASTHLSLWKADLAEENSYDEAIQGCEGVFHVATPMELVHQGQDGEEEIESTTLNGILSIMRSCSKPKTVKRFVYTSTTGTITVQPQPPLHEYTEDLWTDVDLCYELKMYGWMYLVAKTTAEKAAWKYAEENGINMVTVHPSIVLGHFITPHTSFSTDVATAFYTTNMALLKKIHRSPAVHLDDVCNAHIYLFEHPLAKGRYICPSHTVNLFEIAHSLGLKYPDRNIVTEFEGLDRSPKIIPCSSKKLMALGFEFAHKNKDVGDFCAEIIAQRNGFALMIEKLNFQIFELFNGLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1F5</accession>
    <name>A0AAF1B1F5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0519741</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1F5"/>
    <dbReference type="KEGG" id="dcr:108221366"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH00382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="541" mass="62123" checksum="9BEA58E771C81AEB" modified="2024-05-29" version="1">MTVGLRSIVFDAAKVNIGCSKTFSLVKEMTGGYANVGATLRDFRNFDRDLNEFVGERDGQMLIDEFRIMQETSKSFYFAHEVDAEGHLTMLFWADAVGRRNFEIYGDAVSFDTNKYNMIFAPFTGVDKHDRCVTFAACLLSKEDICHYNWAFKQFVKAMGRNPVVFITDQCPAMKVAVSVSFCGDNDLIPSKHRLCMWHIMQKFPIKVVNRLCKETDFMEKMKKYIWSSNLEIEEFENGWQSVIKEFNLEDNKWLADMYEIRKSWIPSYFRDNSMFGLMGTTSRSESENFFFGQFHRQGKPNTLSRWFLEDDAADLFTRTIFYKVQEEILASCLEMQIKRMSEEVDGVTQLDIKDVKVKDKLFKVAVSKTHAVCSCKKFVMCGILCRHAFCGLKQIGVIKFPRSLVLNRWMKIVECGTLSQSVAAGVQMDKLDYVRKIIKQISSDLENSGGDGADFSKRDHIAAMVGEQPVGDVSILVPNVCKNKENYFKRLISEREREKALNKANKRIRRCKECSGTNHDSRTCPRKKTGSTDARAENQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B869</accession>
    <name>A0AAF1B869_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0727696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B869"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF81">
        <property type="entry name" value="OS06G0658400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="319"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="51903" checksum="846D752056D4F899" modified="2024-05-29" version="1">MVASAAGPMFLKSVNASQVKVKDAEYIAALFMEIVEEIGPEHVVQIVTDNATNYKATGALFETKYPRIFWTPCVVHSLNLAIKYICEPSEKSDYYPHCAWIKELVTQVREINYFILNHSLPRMIFGRYSEVKLLKVAETRFASNIVMLRRIRRVKEGLEKTVMDTLVSDTWWDKIDYFLKFTEPMMRFFRVADKDSCILHLVYDMWDTMIEDIRSCIFDHEDEDLLIGNSAFFNDIHKIIEDHWNKSNTPLHCLAHCLVPKYYSQEWLQGGGCGIRRVAPNEDEEISLNREACLRRIFDKPEDLKKVTKEYGAFACELEYFSQPHVMASRGEDHMSWWANYGSFTPLLQGLAFKLLSQPASSSYCERNWSTYGSIQSVKRNQLATQRTEDLVYVHNNIRMISRKQPEYTSGPCKYWDIGGDNFDGDNILEIADLSLDDPEIEAITFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8G5</accession>
    <name>A0AAF1B8G5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0727196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B8G5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000459">
        <property type="entry name" value="ARM repeat superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH07762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1024" mass="113767" checksum="8F065C138610B17B" modified="2024-05-29" version="1">MVDQDQHWLLNCLNATLDTNQQVRSFAETSLNQASVQPGFGSALCRIAGNRELPLGARQISFSAVLLKQFVKKHWQENEENFEHPVVSIQEKETIRSLLLLSLDDNHKKICTAISMAVASVAHYDWPEDWPDLLPFLLKLINDQTNLNAVNGALRCLALLSSDLDDTVAPTLVPVLFPCLYTIVSSPQVYDKQLRARALSIIYSCTSMLGVMSGVYKAETSALLLPMLKPWMDQFSSILDIPVQPEDPDDWSIRMEVLKCLNQFVQNFPGLIESQFMGIMGPIWQTFVSSLRVYERSALEGLDDSYDGRYDSDGAEKSLESFIIQLFEFLLTIVGSPRFVKILGNNISELVYYTIAFLQMTEQQVHTWSLDANQYVADEDENTYSCRVSGSLLLEEIISSCGTEGIHAIVEAARKRFVESQQEKASGSSGWWRMREANLLALTSLSEQEISLLPGCTLGNLLEQILSEDMATGVHEYPFLYARMFSTVANFSTVINNDIVEQFLYAAIKTIGLDVPPPVKVGACRALTQLLPDANIVTILPHFVNLFSSLIDLLKQASDETMHLVLETLQAGVEAGHESTEFSSIEPLISPVILGMWALHVSDPFVSIDALEILEAIKNAPGCIHPLVSRILPYIGPILNRPQQQPDGLVAGSLDLVAMLLKNAPSDVVKAVYDVSFDPVVRIVLQSDDHSEMQNATQCLAAFLYCGKEDLLSWGGDPVFTMKSLLDVASRLLNPDLESSGSLFVASYILQLILHLPSQMAQHIRDLVAALVRRMQSCQIAGLKSSLILIFARLVHISVPQVEQFIDLMISVPVEGHYNAFAYVMSEWSKQQGEIQGAYQIIVTTTALALLLSTRHANLEIVNVQGHLIKSTSGITTRSKAKLAPDQWTIMPLPAKILAILADTLIEMREQFVAGADEDDEWEEVEAEDTETNQNMLYSAGANISGRVKNSYLESMAKSFDEDQDDGFEDELLSSADPLNKINLTSYLVDFIVKFSHSDKPYFDHLCQSLTQPQVKALELVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8K2</accession>
    <name>A0AAF1B8K2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferredoxin--NADP reductase, chloroplastic</fullName>
            <shortName evidence="15">FNR</shortName>
            <ecNumber evidence="15">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0729868</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2 reduced [2Fe-2S]-[ferredoxin] + NADP(+) + H(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Plastid</location>
            <location evidence="2 15">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="15"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B8K2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06208">
        <property type="entry name" value="CYPOR_like_FNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000048">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000008">
        <property type="entry name" value="Ferredoxin--NADP reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015701">
        <property type="entry name" value="FNR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035442">
        <property type="entry name" value="FNR_plant_Cyanobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43314">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501178">
        <property type="entry name" value="FNR-PetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000361">
        <property type="entry name" value="Frd-NADP+_RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 15" id="KW-0274">FAD</keyword>
    <keyword evidence="7 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="83"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="282"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="321"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH10399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="40452" checksum="F3160CC7A7D88CD5" modified="2024-05-29" version="1">MDVAVSAAVCHPFPKSNALSNKSSIIRPKRLCFTKVPVVYRNASSGGRVGSIRAQVITEAPTKVEKVSKKMEEGVITNKFRPKDPYVGKCLLNTKITGDDAPGETWHMVFSTEGELPYKEGQSIGIIADGVDKNGKPHKLRLYSIASSALGDFGDSKTVSLCVKRLVYTNEQGEIVKGVCSNFLCDLKPGADVKITGPVGKEMLMPKDPNATVIMLATGTGIAPFRGFLWKMFFEKHKDYKFNGLAWLFLGVPTSSSLLYKEEFEKMKEKNPDKFRLDFAVSREQTNEKGEKMYIQTRMAQYAEELWELLKKDTTFVYMCGLKGMEKGIDDIMVSLAAKDGIDWTEYKRSLKKAGQWNVEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B991</accession>
    <name>A0AAF1B991_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0728885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH09428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="105"/>
            <end position="127"/>
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            <end position="220"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="247"/>
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            <end position="481"/>
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        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="540" mass="60691" checksum="BFAA3FFC45384825" modified="2024-05-29" version="1">MERVVEENGKTRGDDEYTKDGTVDLKGNPVLRSNTGRWRATSFIVGYEAFERMAFYGISTNLVLYLTRKLHEGTVKSSNNVTNWVGTVWLTPILGAYIADAHLGRYWTFIISAFIYLGGMSLLTLVVSLKSLRPPSCGDSITDVDCNKQASPFQVGIFYCALYIIALGTGGTKPNISTMGADQFDEFEPTEKTQKISFFNWWVFSIFFGTLFASTFLVYIQDHAGWGLGYGLPTIGLFLSILVFLAGSPYYRHQPASGSPLTKMARVLIATIRKWKLVVPDDPKELHELNLDEYSKPGKYRIEHSPLLRILDKAAVVDGRSPHWMLCTVTEVEETKQMVKMVPILLVSFLPSTLIAQGHTLFIKQGTTLVRSIGPHFSIPPASLVAFITIFMLITVVIYDKFLVPTLRSYTKNPRGIPMLQRMGIGLVMHVIIMIIASVCERKRLNVIEDHGITKKNQIVPLSIFILLPQYALMGVADTFWEVGRLEFFYDQAPKSMKSFGTAYYTTSLSMGNFLSSFILTTVSDFTKRDGHKGWIFWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BBI5</accession>
    <name>A0AAF1BBI5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0832880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47294:SF4">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 26-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47294">
        <property type="entry name" value="OS08G0431150 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH13370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11588" checksum="18B18C9B541A291C" modified="2024-05-29" version="1">MSGQIFCMVMRINVDCNACCRKMRRVLLNIKEVETHLIEKQHLRVSACGRFQPADVAIKIRKKMNRRVEILEIQEFSMNDGSAEQQPDQVMDNTGQQQHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEA8</accession>
    <name>A0AAF1BEA8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0936167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BEA8"/>
    <dbReference type="KEGG" id="dcr:108200565"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019148">
        <property type="term" value="F:D-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000081">
        <property type="entry name" value="D-cysteine desulfhydrase 2 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780:SF7">
        <property type="entry name" value="D-CYSTEINE DESULFHYDRASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="48892" checksum="2F4F8305F73AB43E" modified="2024-05-29" version="1">MRLEFVNKQSLISVALRKDFHSCKFSSSPQVAKKLNLGDEDFVSKLLDRRWALASPETKIHQIKVSRHHKHKQAGYFGNLSFKNNSNPLLGDGMLENSNDHASFYVVRDDLLHPLVNGNKARKLDGLLPLLEDNSITDVVTCGGCQSAHAAAVAVSCAERGLNSHLLLRGEPPETATGYGLISMIYGNATYVPRTLYAKRETMLANHAEFIAGSSGSVVALSDLFEASITNHVSKKPISAQIDAPRLSDNIKKVSIINEGAGDAAGLLGTIRLVHYLSRDHIFGKNQPLKVVVDSGTGTTAVGLGIAALCLGLPWEVTAVMLADTFDGYRNLEQRLVTEFLRFCAFSLCAEVVDRLNDGLVHWLERPHPRKFGNVLKGEVEKCQQIAQQTGILVDPIYTLAAWELSTTLSQEEVGGSKIVMLHTGGTLGMFGLAQRYKPFFRLSEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEQ7</accession>
    <name>A0AAF1BEQ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endoglucanase</fullName>
            <ecNumber evidence="13">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0832670</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="13"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BEQ7"/>
    <dbReference type="KEGG" id="dcr:108198504"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 13" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12 13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041780729" description="Endoglucanase" evidence="13">
        <location>
            <begin position="28"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="15">
        <location>
            <begin position="536"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="499"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH13161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="628" mass="69164" checksum="66FC6996DE2A56E8" modified="2024-05-29" version="1">MVNAVACSCALVLVILVGLQGKSSVFASGFDYADAFDKSLLFFEAQRSGKLPSTQRVRWRADSGLSDGFKQGVDLVGGYYDAGDHVKFGLPMAFSVTMLSWGVTEFRKEITGQNQMGNTLAAIKWGTDYFIKAHPQPNVLWAQVGDGDSDHYCWERAEDMTTSRTAYKLDTKNPGSDLAGETAAAFAAASLAFKPYDSSYSNLLLVHAKQLFSFADRFRGLSINSIPNSKKFYASSGYEDELLWAAAWLYQATKEEYYLKYVVDNAVSMGGTGWAVKQFSWDNKYAGVQILLSKVLLEGNGGAYTSTLKQYQAKADYFTCACQQKNDGYNAPMTPGGLLYINDWNNLQYAASAAFLHAVYAQYLSAANAMVKCPDALLKPQDLLNFAKSQGDYLLGKNPKSMSYIVGYGQNYPNHVHHRGASIASTSVLHSAVGCVQGFETWYRRTERNPNIIYGALVGGPDKNDEFIDSRSNYEQTEPTMTGTAPLIGLFSKLQSLSGKSGADSYHQPPISHPKGAPVPHPKSAPVATPRSATPVEFLHSITNSWTVGKTNYYRHRVIIKNISQKKITNLRLLVENLNGSSWGLSPTHEKNIYVLPEWLKVLKPDSDCTFVYIQSGPQAKISVQSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X381</accession>
    <name>A0A161X381_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0207088</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X381"/>
    <dbReference type="Gramene" id="KZN05445">
        <property type="protein sequence ID" value="KZN05445"/>
        <property type="gene ID" value="DCAR_006282"/>
    </dbReference>
    <dbReference type="OMA" id="RYNIMIS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF156">
        <property type="entry name" value="PLANT SELF-INCOMPATIBILITY PROTEIN S1 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620646" description="S-protein homolog" evidence="6">
        <location>
            <begin position="31"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG87856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="18346" checksum="6F946BCD82BE7CE8" modified="2016-07-06" version="1">MKSIPTSFPSTKSLFLNILTLSLLCSACLCKIHVDISNHLPDGSPSLTVRCQSRDDDLGYQELFPNQSSYTWAFRPAWFSTTLYFCHFFWDGKDAVFDVYNEDWGVDYCAQYVLLSGRYNIMISDTCYWQVTADGIYLSKTSDPVSSEGPWLQMHNWGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XGR6</accession>
    <name>A0A161XGR6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3 13">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0207926</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Plastid</location>
            <location evidence="13">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Plastid</location>
        </subcellularLocation>
        <text evidence="13">And non-photosynthetic plastids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3 13"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XGR6"/>
    <dbReference type="Gramene" id="KZN06179">
        <property type="protein sequence ID" value="KZN06179"/>
        <property type="gene ID" value="DCAR_007016"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206006"/>
    <dbReference type="OMA" id="LFGVQCD"/>
    <dbReference type="OrthoDB" id="1393670at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05333">
        <property type="entry name" value="BKR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000194">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="14">
        <location>
            <begin position="80"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG88691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="33445" checksum="399E36BC9C0483B0" modified="2016-07-06" version="1">MATSVAGSAIAAFKLTGASATTDRKISSLGSFSPISKSFQTLNHSLQCRSLSSFASSGVKAQVSTIEKAAVETVKEVEAPVVVVTGASRGIGKAIALALGKAGCKVLVNYARSSKEAEEVCKEIEASGGQALTFGGDVSKEADVESMIKTAVDAWGTVDVLINNAGITRDGLLMRMKTPQWQEVIDLNLTGVFICTQAAAKIMMKKKKGRIINIASVVGLVGNVGQANYSAAKAGVIGFTKAVAKEYSSRNINVNAVAPGFIASDMTAKLGDDIEKKILTTIPLGRYGKPEEVAGLVEFLALNPAASYMTGQVLTIDGGMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XIZ6</accession>
    <name>A0A161XIZ6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0209050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XIZ6"/>
    <dbReference type="Gramene" id="KZN07169">
        <property type="protein sequence ID" value="KZN07169"/>
        <property type="gene ID" value="DCAR_008006"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209000"/>
    <dbReference type="OMA" id="QINARYS"/>
    <dbReference type="OrthoDB" id="1306371at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040290">
        <property type="entry name" value="Prot_E6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35274">
        <property type="entry name" value="E6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35274:SF2">
        <property type="entry name" value="E6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043500933" evidence="2">
        <location>
            <begin position="25"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="315" mass="36134" checksum="05845F4C8FA55005" modified="2016-07-06" version="1">MASSTTFSYFLLLALLAASLHVHARDSQFFSKVTSNNIPKEVLPTKQQEPLNKQEQEPNFVPLNQQNPGGYGLNGHESGQLPPTTENLPYRTQTENSYNKESNPNLVDGLYYNNDANVNTESYSSSNNGDQFRNNEAYVTNQQGMSDTRFMENAYTTPITSNNNGDRYYNNDAYVTKPQGMNNNNNDNNYNGENMYNNREHDIGEAKLGVTNGNNYNNGGNMYENEKNGMSDTRVMENGRYYYDLNTEKNYNLNNYNNNLNENSRSFNSRNEYANNRGNYRNSFKGQNGNNENFMEGYNGNQEEFQEVRDEQFMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y3D8</accession>
    <name>A0A161Y3D8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0727308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant ureide permease (TC 2.A.7.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y3D8"/>
    <dbReference type="Gramene" id="KZM86669">
        <property type="protein sequence ID" value="KZM86669"/>
        <property type="gene ID" value="DCAR_023803"/>
    </dbReference>
    <dbReference type="OMA" id="IFLPSEW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005274">
        <property type="term" value="F:allantoin:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015505">
        <property type="term" value="F:uracil:monoatomic cation symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030189">
        <property type="entry name" value="UPS_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009834">
        <property type="entry name" value="Ureide_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31081:SF5">
        <property type="entry name" value="UREIDE PERMEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31081">
        <property type="entry name" value="UREIDE PERMEASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07168">
        <property type="entry name" value="Ureide_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="407" mass="44815" checksum="9D2027BE473F299B" modified="2016-07-06" version="1">MYLVESRGGAIACMFLSLFLLGTWPAVFTLLERRGRLPQHTYLDYTITNFLAAVIIAFTFGELGESTAENPNFLIQLTQVNWPSILFAMAGGVVLSLGNLSTQYALAFVGLTVTNVITTSITVVIGTSLNYFLDDKINNAEILFPGVGCFLMAVCFASGLHASNEKDNEAKFKTLANNKIEEIGEQVPIYKEICLISDARDIENVLGTTAKAKVGTADFLLEVENRRAIKVFGKSTYTGLGITFFAGLCFSLFSPAFNLATNDQWETLREDVPHLSVYTAFFWFSISCFVIAIILNISFLYRPVLNLPRSSFKAYLSDWDGRGWASLAGLLCGFGNGLQFMGGQAAGYAAADAVQAYPLVGTFWGIILFREYHRSSRRTYTLLIIMLLMFIVAVGVLMASSGQRKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y607</accession>
    <name>A0A161Y607_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0206314</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y607"/>
    <dbReference type="Gramene" id="KZN04777">
        <property type="protein sequence ID" value="KZN04777"/>
        <property type="gene ID" value="DCAR_005614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF132">
        <property type="entry name" value="TF-B3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG87092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="531" mass="60174" checksum="3AE9AB678D361BD0" modified="2016-07-06" version="1">MAASAGSVLGSTSGVKTVPGSSLMDIDEEVRFVCPNFVSDYIFGVLKLPEEFCVGKGQDLPERLLLHVPPGVVWNGIYRKGRQSIEGVEKMMSFYCIKPYHLIVFDYIGGPSFNLKIFNPYGVEINYNFFKNSDTPTGLEGCILNPSEIEVDKLGGTLSYNVYHSGRSICEVVLGNKHIRKTEVYKVLKRSDWESLGIVESMESVRLSFRNITWLVKLVWRNGKLYFDRGWYKFAKAGKFGIGDAVVFHKTDWPQKFIATVFENEVLSKCNVSGVGQRDGVMEWFKMASPTFICTGEMEIPRVFTNLSQFKLDETVKLILRDGETISVKFCSESNFLFGMRNLVRLYSIESTDVMVFTLVRQSTFVLSIFKFYGMESEYNAVEVCKTEAMKKVCLEDIIILSDSDNSEEGMAVEQEQDAEEEGEGIGEEADEMPEANVSFRVTLKPSHVDKRQHGVYFPSSLYSTYKRWTNMTNIRLICGAKVCFVSVLRTGKVCRFGRGWSEFTTANELSEGDILQLNYIDHLTFQVEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YA29</accession>
    <name>A0A161YA29_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0730302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Alfin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YA29"/>
    <dbReference type="Gramene" id="KZM89319">
        <property type="protein sequence ID" value="KZM89319"/>
        <property type="gene ID" value="DCAR_026394"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF98">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="288" mass="33296" checksum="BC6035125ABB5062" modified="2016-07-06" version="1">MAARESQQNIPRACNVEDIFHDFLGRRVGLLRAFTADVRDFYLTCRSVKESLRLYGLPDKQWELVTAATDKWELREIPAPVLGINLHLKRMHVREWVSLVALHCDSWLLAVAFYYAAKSGFDKADREQLHHMINNLPTLYELFGWEEPETQSSTNNKGSPKVGCDSLKDRDKSKREGQDENQVAKDEEEEVQVEEGEEAHSCPHFNKIKRADLEQGHKRRSCRRYNKVADVFQLYMLCECDPGNGNLRWYGLPDGQWEVSDTPSEASPRNLPKSPFINFVRDVMPEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YAM3</accession>
    <name>A0A161YAM3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0622910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YAM3"/>
    <dbReference type="Gramene" id="KZM89529">
        <property type="protein sequence ID" value="KZM89529"/>
        <property type="gene ID" value="DCAR_023108"/>
    </dbReference>
    <dbReference type="OMA" id="KESHETY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF2">
        <property type="entry name" value="OS06G0560700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH03511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="23863" checksum="AC4967236A0CB5F0" modified="2016-07-06" version="1">MQKKYKRWECGNDNIMNRMFVFGDSFVDNGNSNFLETVANADYLPYGMDFPLRPSGRYNNGKTFIDALGDLLNITAFIPPFFDPSTKGNHTIDGANYASSGSGILNETSSTLPPKQGCTTKEEGTAIDPIMKFLSFGDVKNPCCQVVSTEDGGNDTLCTKGGAMCKDRNQFLFFDGLHPTEAVNVVLATKGFNSNLTSDVYPFSIRKFTEIWCHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YD04</accession>
    <name>A0A161YD04_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0624088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YD04"/>
    <dbReference type="Gramene" id="KZM90559">
        <property type="protein sequence ID" value="KZM90559"/>
        <property type="gene ID" value="DCAR_022076"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225806"/>
    <dbReference type="OMA" id="IYDPDMF"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH04677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="83" mass="9487" checksum="D4166998B240490B" modified="2016-07-06" version="1">MSYDDVEIEDMEWNAELEAYTYPCPCGDLFQITKEDLKLGEEIARCPSCSLFITVIYNMEDFAGDNDRNKNLEPTKQQPVAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZTC9</accession>
    <name>A0A161ZTC9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_022757</name>
        <name evidence="6" type="ORF">DCAR_0623287</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM89880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZTC9"/>
    <dbReference type="STRING" id="79200.A0A161ZTC9"/>
    <dbReference type="Gramene" id="KZM89880">
        <property type="protein sequence ID" value="KZM89880"/>
        <property type="gene ID" value="DCAR_022757"/>
    </dbReference>
    <dbReference type="OMA" id="LKCACPD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056562">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056563">
        <property type="entry name" value="LysM3_LYK4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF10">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23472">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23473">
        <property type="entry name" value="LysM3_LYK4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831613" description="Protein kinase domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="4">
        <location>
            <begin position="179"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="273"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM89880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH03886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="69211" checksum="E103277AA3B4F036" modified="2016-07-06" version="1">MIPTLWFLILSHTYFCYAQQFYDPTLCDSHTNNPGTRYACNSFQKSCQTFLVYRANQGFQTIFNISSLFNVISHDELLSLNNLTSSTQILEPGTDVLVPIKCSCTGQFFQANLSYYVAYNTTFANVACGVYEGLVKSSILVEENEFQGNVLNIGSVLNVPLKCACPDNVSSVAGYKYFVTYPLVEGDYTNKVAQKFDIAGEDIWEANHIDSLVTVYPNTTILVPLRSEPRIRFISPDSQPPTPGFLPISPIERTKSIKLKKLYIAGSVIGFSLVLLTLLGCGIYVKALRRWKGEKFHHSSARRSSMTSFSTPLSSPLSVMTPRSSTNSCLSPDLLIGLKYTLCSYSTEEIREATNDFTEETKINGYLYKGFIDDAEVMIKQMRFEDIRQVIDLHSKMNHVNIMKLLGVCYGESDFSWSYLVFELPKNGSLRDCLSKPLKSLKWIKRTHIAYDLAKALHYLHYCMVPPHTHMSINSTNIFLTPSWRAKLAVSGSTPSVGCSKENEIMSSAGGWIAPEHLLNGLVNEKVDIFAFGVVLLELMSARESVEGRSFRESIGFLGGGASEGGCFEQLRSFMDPSLKEDYPLAEALCLAVLAKACVEDDPMHRPSMDDIIKVLARMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A3W3</accession>
    <name>A0A162A3W3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_019064</name>
        <name evidence="6" type="ORF">DCAR_0521814</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A3W3"/>
    <dbReference type="STRING" id="79200.A0A162A3W3"/>
    <dbReference type="Gramene" id="KZM95822">
        <property type="protein sequence ID" value="KZM95822"/>
        <property type="gene ID" value="DCAR_019064"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224038"/>
    <dbReference type="OMA" id="FEVRQWD"/>
    <dbReference type="OrthoDB" id="26838at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02645">
        <property type="entry name" value="R3H_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001088">
        <property type="entry name" value="uncharacterized protein ycf45 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058670">
        <property type="entry name" value="PTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034081">
        <property type="entry name" value="R3H_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045735">
        <property type="entry name" value="Spore_III_AA_AAA+_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953">
        <property type="entry name" value="KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953:SF14">
        <property type="entry name" value="PROTEIN SEEDLING PLASTID DEVELOPMENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25516">
        <property type="entry name" value="PTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19568">
        <property type="entry name" value="Spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="216"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM95822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH02425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="691" mass="76057" checksum="6A5B71A14B86D541" modified="2016-07-06" version="1">MSALNSHFVLIDVHNSWHSATHIPSATLAYLNAVSKFSTFSAANRRTGGSHVKVSSCNSSEVIRSPEIRRPRDNWSAPGNGLSSVSSNTVSTSGSESVPELNLFLELVPLRMRNELYRHQEIGDLIEIVMDLGRVPLARFPSGDWVISEQPVKLEDLQHAISKVGDFSDDNRSGINNSLHRISAIRNRKMQIIGLTCRVGRAVSGSAEIIRDLIEGGGSILVIGPPGVGKTTLIREIARMLADERKKRVVIIDTSNEIGGDGDVPHSGIGRARRMQVPNVNMQHNVMIEAVENHTPQTIIIDEIGTELEALAASTISQRGVQLVGTAHGMTIDNIMKNPSLQILVGGIESVTLGDEEARKRKVQKTILERKGPPTFTCAVEMISRTECRVHHRLDATVDALLKGKSPLFEIRHMDAEANNKYLEFTSTPELSRLESGLLNDVKGLETESDDEDFSGSIETSSNAERKRSSPVYVYTYKILEAELQQVAAVMGLEDEIFVTEDIGLADALLATSSELKQNPWIRSVAKFQKLPVFVIKSSNMAQMVKAIRMILEKFSFVAKSKKPVKGSPDFEIEDDAPKRKPSLEEIDALEEVRLAIEYIVIPGGEPVELLPRRSEIFALQLELVESYQLAVENTGTEMNPRMQILPHKLNKRTSANNSKRSASLQEGFSIQAVPNKGVGTSVPQLPLLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5F4</accession>
    <name>A0A162A5F4_DAUCS</name>
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            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
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        <name type="common">Carrot</name>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH03137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A162A5F4"/>
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        <property type="protein sequence ID" value="KZM96462"/>
        <property type="gene ID" value="DCAR_019704"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
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    </dbReference>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03137.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="413" mass="47916" checksum="1AD82D30DD58B39C" modified="2016-07-06" version="1">MEKPTKKTDCEEDEHRRGIDSLPDEITLDIFSRLSVTSLMQSRFVCRYWKNFSFDRNLASLHLSQAVKRDPLLIFHSDFPIRNQLCFAEFSGTDDDAQGIVKKISTPFSASMPEFTVVSSCNGLLCLFDSLYKDAVYIYNPFTRDYKELPKTRQYDEETVVCGFGYHPETNQYKVVKIVYYWIVNFGYPRAHSRIRMSRNSKSEVFVLSLGGNAWRNIGEAPYFIQRRPQGALFTCGRLHWQTQGWFKNVRGFVIISFDLADETFHEIPRPDFSRIADGHRYHLADLKGCLSAIVYICRSKELEIWVMREYNVKESWIKEFRIGANIPDSPSTKLLQPFRMWRNSLHRALVRILCILENGEILMEYSVGRLALCDVLSGTYKDITFKGMPSIFQTVVHVGSLNLIDFPVICNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AKP9</accession>
    <name>A0A162AKP9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AKP9"/>
    <dbReference type="Gramene" id="KZN10366">
        <property type="protein sequence ID" value="KZN10366"/>
        <property type="gene ID" value="DCAR_003022"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195898"/>
    <dbReference type="OMA" id="DVIATKM"/>
    <dbReference type="OrthoDB" id="1939646at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012417">
        <property type="entry name" value="CaM-bd_dom_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33349:SF20">
        <property type="entry name" value="CHROMO DOMAIN CEC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33349">
        <property type="entry name" value="EMB|CAB62594.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07839">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CaM_binding"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="46727" checksum="5EA87E392AC1BDDC" modified="2016-07-06" version="1">MATRAKDTTPGIVKEKRRITHSPSFPRPANTTKKSVNTATTTTIAPNHIKPTKPSNVDPSIKPVSTKASTSTLQKPDLARRKSFGSRPLSQPKSPDPAANAEKSPKTTGTGVRPKPVTQRSRSSSSLKTPASPSARPKVAATVTRNPVPGKSARPATRPATTAVYRKKEPQSNAAAKTLKSPTSKANRDSPQKSESIDEIDATKPDHEVETESCQDIHDQESVCSSVVDAHNENDDTLLNVDLEHDDQLDQINEEAYLLSEASTVVSEIEQSEVELENKVNEVTEKLQQESTNNDEPKIDADNGNDASSPKDGLQESEEVNMSTNETNEKDPVAAPEEEKEAVDDKNEKSGDEKEKAVEQVRPEGIEQVKVDKEDTTKKINNNNTTGGSDKKESPVSNEVIEETASKLRVKRKNKVLALVGAFETVMSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TZY0</accession>
    <name>A0A164TZY0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_025303</name>
        <name evidence="5" type="ORF">DCAR_0729055</name>
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    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="79200.A0A164TZY0"/>
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        <property type="protein sequence ID" value="KZM88228"/>
        <property type="gene ID" value="DCAR_025303"/>
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    <dbReference type="OMA" id="KYCVDFL"/>
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        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="CCDC174-like"/>
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    <dbReference type="PANTHER" id="PTHR15885">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
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            <end position="168"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM88228.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="260" mass="29467" checksum="B684A40076819004" modified="2016-07-06" version="1">MAEKDEQEAGSKKRVSSTYESLGWLTESTVMPKKLKPIEGVGASSIFELKAQLYRSQEESKRLGKDSAQPSSSFEHLDVHKAKKKILAHDPFANKNHGVESRAHKDKLELKALNEGAVSYAALEKKAELYDKLARGELSDEEDKEKYCVDFSSKIVQEEPQHPQDHDTSGTGQTEYGGSSMRPNIMPMGLGRAGATFDNEEHKRFVKEVHEEVNQAREKVSEAKLRRQEQMALHREKLKQAYLRKRLEKIKASKTEQNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UGU3</accession>
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        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">DCAR_0729798</name>
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    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM88865">
        <property type="protein sequence ID" value="KZM88865"/>
        <property type="gene ID" value="DCAR_025940"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196213"/>
    <dbReference type="OMA" id="YSHEVEF"/>
    <dbReference type="OrthoDB" id="540873at2759"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH10331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="102" mass="11466" checksum="A9475B4249CF08D7" modified="2016-07-06" version="1">MEAQFFRFLKIVGVGFKARAESGGRLLFLKLGYSHEVELTVPPAVRVFCFKQNVVCCTGIDKQRVHQFAAAVRNCKPPEVYKGKGIMYLDEVVKKKAGKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YNT2</accession>
    <name>A0A164YNT2_DAUCS</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YNT2"/>
    <dbReference type="Gramene" id="KZM94787">
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        <property type="gene ID" value="DCAR_018029"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223712"/>
    <dbReference type="OMA" id="NACWARR"/>
    <dbReference type="OrthoDB" id="1899156at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="entry name" value="At1g16860-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33709:SF4">
        <property type="entry name" value="OS08G0230200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33709">
        <property type="entry name" value="OSJNBA0035M09.9 PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="261"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
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        </location>
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        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH01260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="481" mass="51067" checksum="125E801D7691577A" modified="2016-07-06" version="1">MGSRFPSHQLSNGLYVSGRPEQPPKERTPTMSSTAVPYTGGDIKKSGELGKMFDISMDGSRSRKSGPITSGPSRTGSFGGAAPHSNPMTTSSGGRMGSGSIAGVPGSISMKKTSSGPLNKHGEPIKKASGPQGGANAASRHNSGPLPPVLPATGLITSGPITSGPLNSSGAPRKASGPLDSTGSLKSGSASIVQNQAVTHLSLESENSFFKSFPKLIFWSIVLLFVMGFIAGIFILAAVGNPILLVVVVVLFVAVTSVFVWNSCWGTKAITSFVARYPDCELRTAKNGQFVKVSGVVTCGNLPLESSFNRVPRCVYTSTSLYEYRGWDSKAANSSHRRFTWGLRSVERHVVDFYISDFQSGLRALVKAGSGAKVTPFVDESIAVDINQSNRDMSPEFVRWLGKRNLSGDDRVMRLKEGYIKEGSTVSVMGVVQRNENVLMIVPPPDPVPTGCLWSKCILPAHLEGIVIQCEDSSKVDAIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZU39</accession>
    <name>A0A164ZU39_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_019646</name>
        <name evidence="10" type="ORF">DCAR_0522469</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A164ZU39"/>
    <dbReference type="Gramene" id="KZM96404">
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        <property type="gene ID" value="DCAR_019646"/>
    </dbReference>
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    <dbReference type="OMA" id="HSAKNAD"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013909">
        <property type="entry name" value="NuBaID_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF16">
        <property type="entry name" value="F20D23.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NuBaID_C"/>
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    </dbReference>
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        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3HC-type" evidence="7">
        <location>
            <begin position="81"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="NuBaID C-terminal" evidence="8">
        <location>
            <begin position="860"/>
            <end position="914"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="290"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="459"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="770"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="825"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="784"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM96404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH03077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="959" mass="101663" checksum="72523B775D9BBEF4" modified="2016-07-06" version="1">MREEGISSSGDPLIAPTPAASSAGASSPAVPTNVGSIDWLGYGQSSKAASVSRIVSRSARPSLSTSAGGSALGSSQPSCRPWERGDLLRRLSTFNPLNWSGKPKAASSLACARRGWVNVGVDKIECESCSANLKFSAPSTWMSTQAESAGEEFAKKLDGGHRINCPWRGNICAESLVQFPPTPPSALVGGYKDRCDGLLQFLSLPIVAASAIEQMKASRSLELERFLDQLQDLTVGDSGLKADVLPQTEANREEVSSVYTHAQKLISLCGWEPRWLPNIQDCEEHSAQSARNGYSVDHKDSGNLNDPGLGKKALSSSTKKDSKKKEVMCSKSNGDSRSPLLDCSLCGATVRILDFLSVTRPARFAPSIVDMPETSKKLALTRGASAASGINGWIAADGMGKEQTEEHDEAATADEGKSMSNVGVDLNLTMGGGLPSAQVHMNVVTDQPQEITIGRDLMIGQPSSSEVGDRAASYESRGPSTRKRNLEEGGSTVDRPQLLVRQADSVEGTVIDRDGDEVNDGEQYSAGPSKRSREAYAFETYQSSYRRDSSGAGPSRSLVFEINRDGNREDLYNQRDDQSFGIPSARDSARASSVIAMDTIGHSADDDSMESVENRPGDMDDVQFPMAFRNPDLNETSELNYSNQAQQSVCLPAVRGAGDVGVSSTNEEEVLNTDTTTARGRDGPSFGISGGSVGMGASHEAEIHGADLSVHRADSVVGDMEPVAEVTENQGQTGEFAPDPGLMGDYVPQEMDREYPHGDSQDLMSGSVARADSGSKVVGSGKVESVESGEKTSDMHLLPNETSNHPSHSCNAILCSGYEASKEEVTQAGKASPTNDSGYPKADDMVANGKELPNGESNYEEAVEFDPIKHHNYFCPWVNGNVAAAGSSSNSGSGSGASAIALCGWQLTLDALDSFQSLGQAPVQTVESESAASLCKDDQLTPGRKFLALNSFSRSHGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZU48</accession>
    <name>A0A164ZU48_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_019648</name>
        <name evidence="5" type="ORF">DCAR_0522472</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A164ZU48"/>
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        <property type="protein sequence ID" value="KZM96406"/>
        <property type="gene ID" value="DCAR_019648"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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        <property type="entry name" value="Reg_chr_condens"/>
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        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
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        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF365">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION (CELL CYCLE REGULATORY PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RCCNDNSATION"/>
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        <location>
            <begin position="27"/>
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            <end position="442"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM96406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH03080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="442" mass="47245" checksum="B201DA681E0B5713" modified="2016-07-06" version="1">MLRFVRRGNKSLSHLTTGLTQIQKRWNTVLSFGDGSNGALGLPTSVSVMGSDAYEPTPVPGLPSDVSAVAAGHYHSLAVTSRGQLWAWGRNNEAQLGRGFNSPRETWYEPKRVEELDQVVVQYAFASGVISAAIGVDGSLWVWGKSKRGQLGLGKGVTEAVIPSRVEVLAGEEIAKVSLGWGHALALTKDGKLFGWGYSADGRLGRIGDTEESPLESRAGMLNLTEGNKSSTLETAENLVLEAMEKEKDMPIIWEPCLIEELRGIEVTDVACGLDHSLILCNDGTLLSSGSNIYGQLGRCRQDLGLLPVDMNFHPISVASGLGHSLAICKNLSEEKANVATHVVSWGWNASSQLGREGPENLPEIIEALAEETPVSVSGGRVHSVALTSKGEVWVWGCGRNGRLGLGSSTDEAEPVLLDNLEDMNVLQAVSGFDHNLVLASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZV91</accession>
    <name>A0A164ZV91_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0522530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZV91"/>
    <dbReference type="Gramene" id="KZM96461">
        <property type="protein sequence ID" value="KZM96461"/>
        <property type="gene ID" value="DCAR_019703"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222748"/>
    <dbReference type="OMA" id="WGKEYSI"/>
    <dbReference type="OrthoDB" id="1894463at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="418" mass="47823" checksum="689CD0278063D8C7" modified="2016-07-06" version="1">MMDFNMEKPTKKRDCKEDEHRSGIDSLPDEIALDIFSRLSVTSLMQSRFVCRYWKNFSFDQNLVSLHLSQAGKRDPLLIFHSDFPIRNQLCFAELFGTDAAAEGIVKKISTPFSASMPEFTVVGSCNGLLCLFDSLYKDAVYIYNPFTRDYKELPKTRQYDEETVVCGFGYHPETNQYKVIKIVYYWIVNFGYPRARSRIRTSHNSKSEVFVLSLGGNAWRNIGEAPYFIQRRSQGALFTCGRLHWQTRGGSKNVRGLVIISFDLADEKFHEVPRPDFSCVADGHTYHLANLKGCLSAIVYKYGSKELEIWVMKEYNVQESWIKEFKIGANLPDSPSTNLLQPVRIWRSSVHRALVRILCILENGKILMEYSAGKLALYDVLSGRYKDITFKGMPSVFQTVVHVGSLNQIDVPIIGNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WWT9</accession>
    <name>A0A165WWT9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0415933</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WWT9"/>
    <dbReference type="Gramene" id="KZM97737">
        <property type="protein sequence ID" value="KZM97737"/>
        <property type="gene ID" value="DCAR_014901"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216890"/>
    <dbReference type="OMA" id="GMCEYFK"/>
    <dbReference type="OrthoDB" id="5399929at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="847"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1069"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="850"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1080"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG96597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1147" mass="129557" checksum="7F05EF4C460BF54C" modified="2016-07-06" version="1">MANFLEQLAKIKTLLTTSASTAASHHQISEKALAYSTLLHLQEQATDDPALIQSLADSCNYLISFILVDISSHDEEIAAQALKCLGFMIYHPSVVSVISANDASKILEILEKLITTTHIKSVCNLGVWCISIQQLNLSFLADKHHSLLRAIIHALDNPIGSLSTTFEAMQAVVKLTTQLSDMMKDTSHIWAPPIYRRLLSIDRRERDMAQRCLLKIKSTIYPPELALAKVVSIDMKRKLLPTMKELLSQGLKIQVLQAWGWFICLIGPYAVKNRHLINEMLKIPEQTFSDLDPQIQIASQVAWECLIDALIQPPKQAPETSSGHNIDKAKNPLESNEFETDKLLKRIKLIMTPLTGIMSSKCDVSVHLSCLKTWCHLLQNLDTYVNCPSVFKFVWEPMLEIVFRIRPDNKNMLLLSICTDLLDNFASTRSKDLRYDLNDQISSLLSSEIQIPGPLFSGELSWKQCSVKWLPWDLTQLDFYTNMLDILISHSSAISIAPEIKCLLCAAALRIFRTVLRGVKIILMDSSITYEETMVCIRKIVMFVNKICEVAVDDSENNHLHRISLQFVKAVTEELDAFILGSPLYKIALDSKFVNNLRTVSDFRRGKMPGTLSVDHLNMVTPIVCLTVCYMCVLIRLTLDVPEAGFAIQEMSGYLRSILSSYSPLEILQAINSLLYNHLVSECLDAWVVVANCLKDYLDSVKDLSVLETESDKHGYTVLCHFLCYPVAVFYISQRQSSPAKTNGSLQSSICLKAQRKHGLEIVVENWRLLYVSVNKASQPKCFSSKSFTEDLCSMLNWWLDERRDNIDTDSELIQIEKVRDYEIFYLCGEIVISVFEQSMISEKKGGTTTGEIGSSRTSTTGEIGSSRTSNSINNILQFTSRYVSLPWAKVPSTASIDLAVVSRVFSTLVHFLDCLFFQEQIISFMEIMGQPLCEWISHPELQDESSRYQLQLLWVKMLNSLRSSWPPINFDSSFLKLQAPLLESTLDHPNNAISKPTMKFWNSTYGEQTPLNYPRSLVPILDKLSRNGAIKLCRASHFKLDSIQGYKVTTTPNPGSKRVELAEGTQKNCHHNNSSLKRKTPELTEHQKEVRRAQQGRVRDCDGRGPGIRTYTSVDFSQANEESQESQEIRNADAILEMLRKDGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z7H8</accession>
    <name>A0A165Z7H8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_012930</name>
        <name evidence="7" type="ORF">DCAR_0418173</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z7H8"/>
    <dbReference type="STRING" id="79200.A0A165Z7H8"/>
    <dbReference type="Gramene" id="KZM99708">
        <property type="protein sequence ID" value="KZM99708"/>
        <property type="gene ID" value="DCAR_012930"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215545"/>
    <dbReference type="OMA" id="SASTNCQ"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000041">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF119">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A 59 KDA REGULATORY SUBUNIT B' ZETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM99708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG98828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="542" mass="61239" checksum="A4D3D68DC8D347CB" modified="2016-07-06" version="1">MFKQILGKLPRKTSSKSSRSNLSNEGVFNSVSLAGGVSNSSQSNSGSRKAVNSGSGGSRPSNGTHVQEPMSTSVKSEQGKKVGLMGSQADQRRENVVYEALPSFKDVPNSEKHNLFVKKLSMCCIVFDFTDPSKNLKEKDIKRQTLLELVDYISSVTSKFNEITMQEITKMIAVNLFRALPNHDNKVLDVYDPEDDEPTLEPSWPHLQIVYEFLLRFIASPETDAKLAKRYIDQSFILRILDLFDSDDQREREYLKTILHRIYGKFMVHRPFIRKSINNVFFNFIFETGHHNGIAELLEILGSIINGFALPLKEEHKLFLVRALIPLHKPKSISVYHQQLSYCIAQFVEKDCKLADPVIRGLLKYWPITNSSKEVMFLGELEEVLEATQAAEFQKVMVPLLRQIGRCLNSSHFQVAERALFLWNNDHIRTLITQNRRVILPIIIPALESNTRSHWNQAVQSLTLNVKKVFSDADQALFDDCLSKFEEYQIKEKETLSKRLSTWKRLEDVAASKAGSNGGLLDSSFASVAISASTNCQTAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z9Q1</accession>
    <name>A0A165Z9Q1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0418253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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            <tissue evidence="1">Leaf</tissue>
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    </evidence>
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</entry>
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    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
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</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="gene ID" value="DCAR_009606"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="WOG91600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="89" mass="9983" checksum="BEA55E17D51AD4B9" modified="2016-07-06" version="1">MDCSNNNHMNPSHPLLPLLLPQPPYYSPAPSPFSASLTPTSLYLFISLPAATLELQHLAAPTPRCHRFISMSQSQPICYRKQLRLPQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AG57</accession>
    <name>A0A166AG57_DAUCS</name>
    <protein>
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            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ORF">DCAR_0311236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_009952"/>
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    <dbReference type="OMA" id="PIADNCY"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rap30/74 interaction domains"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="30339" checksum="EC0BDEDBAC1D2A8A" modified="2016-07-06" version="1">MEEEQKSSNNNNGSVNKSKLETGKSERSVWLMKCPVVVSNSWKTAADNEPISKVVVSLDPLCPESPSSLQFTMEMANNEAGKMPKSYSLNMFKDFVPMTIFSEVNQGKVAVEGKVEHKFDMKPHNETMEEYSKMCRERTNKSMIKSRQIQVIDNDRGVHMRPMPGMIGFIASNSSSKGKRPAVPVKAPDVKRTRRDRSELEDIMFKLFERQPNWTLKQLVQETDQPAQFLKEILNELCVYNKRGSNQGTYELKPEYKKSAEETAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BQH4</accession>
    <name>A0A166BQH4_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0313007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_011503"/>
    </dbReference>
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    <dbReference type="OMA" id="FYPELYG"/>
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        <property type="component" value="Chromosome 3"/>
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    </dbReference>
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        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG93720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="371" mass="41813" checksum="56D76E07B134276B" modified="2016-07-06" version="1">MSELDPAPAPAPAAGADPLSKAYGSLNEVSPDLSVLHDLACRGSWLTILDKVIQARPHLSKPHHHLTYFSYNLLSLIKLRRFSDALNQLDSLDHSLDSPNYLYQTYPKHYPNQCGSMVPFILRVLHAELPAKVSGNSRQETLDRLYVLLEFVRGKLVSDILSSTSRALWEKRECFVINSIISHHLSLKQYGVCLDLIKYLIQKSESDGEGDDHKLDHRMGLLSKLGYIQMQYGDLDGAKATFGLIENQVDDRVELRNLVGRNKALIYLVGKDYVSAVREYDECIERDGNDVVAINNKALCLMYLRDLSDSIKVLESSLERIPTMALNETLVVNLCSMYELAYVNHADIKKTLSNWIARVAPDDFDSTCTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BXL9</accession>
    <name>A0A166BXL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SNARE-complex protein Syntaxin-18 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_011775</name>
        <name evidence="13" type="ORF">DCAR_0313297</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BXL9"/>
    <dbReference type="STRING" id="79200.A0A166BXL9"/>
    <dbReference type="Gramene" id="KZN03019">
        <property type="protein sequence ID" value="KZN03019"/>
        <property type="gene ID" value="DCAR_011775"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210800"/>
    <dbReference type="OMA" id="FVFQCRE"/>
    <dbReference type="OrthoDB" id="342981at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000039">
        <property type="entry name" value="Syntaxin-81 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019529">
        <property type="entry name" value="Syntaxin-18_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10496">
        <property type="entry name" value="Syntaxin-18_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="SNARE-complex protein Syntaxin-18 N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN03019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG94007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="36365" checksum="C3B09114BF9A506D" modified="2016-07-06" version="1">MAKIRDRTEDFKDAVHRVALSLEYDEPKMAAVMATFIMHKPRQRSPFIKAALKTLESIGELQQFLMKHKKDYVDMHRTTEQERDSIEHEVTIFIKECKEQIDVLRAGISNDEANSKGWLGIRGDSSNTDTVAHKHGVVLILSERLHVVTSQFDQLRAFRFQDTINRAMPRRKVKKVAPTDTTNSMKHNDYQGRGHINSDVAEPSEIQAEPKRIQQQLLDDETRALQVELSGLLDAAQQTETKMVEMSALNHLMSTHVLQQAQQIELLYDQAVEATKNVELGNKELSKAIQRNSSSRTFLLLFLFVLTFSVLFLDWYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CVQ8</accession>
    <name>A0A166CVQ8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0205882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG86665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_005236"/>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:O-acetyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TBL"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
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    </dbReference>
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        <property type="entry name" value="PMR5N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG86665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="360" mass="41014" checksum="1DC784101278F7AC" modified="2016-07-06" version="1">MRFCYSLALPILLALFIIQTEQSVYNETGLRGKKLGGGGTCNLFQGRWVVDSSYPLYQSSSCPFIDPEFDCIKYGRPDKQYLKYSWQPDSCNLPRFNGLDFLKRWRGKKIMFVGDSLSLNEWQSLCCMVHEWVPNSRTKYVRTNLISSVTFEDYGVSIMLYHSVYLVDMVRESIGTVLKLDSIMGGNAWRDADMLIFNTWHWWTHTGNSQPWDYVQDGTTVKKDMDRLTAFYKGLSTWARWVDTNIDPSKTKVFFQGISPTHYQGREWSSASKSCYGELGPLTGSTYPAGEPSASLVLKKVLSSMKKKVYLLDITTLSQLRKDGHPSAYSGDHSGVDCSHWCLPGLPDTWNQLLYAALVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DKG0</accession>
    <name>A0A166DKG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_006191</name>
        <name evidence="5" type="ORF">DCAR_0206982</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DKG0"/>
    <dbReference type="STRING" id="79200.A0A166DKG0"/>
    <dbReference type="Gramene" id="KZM80302">
        <property type="protein sequence ID" value="KZM80302"/>
        <property type="gene ID" value="DCAR_031910"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN05354">
        <property type="protein sequence ID" value="KZN05354"/>
        <property type="gene ID" value="DCAR_006191"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208363"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="192"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN05354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="300" mass="33518" checksum="EC416D62B76B87A4" modified="2016-07-06" version="1">METPMLGVANLDASVTDSDLYDHFSNVVGYRVIWAEVRKDRRTDQCLGDGHVCCNNHQQAVYTIDALNGTTLKGKTIVVTPSTFTFNEVTVELCVPPRTFNMEIPLRRNFKEFGKILLIKVYEDMFSNTYGSIIFDSLQSAASAANELLRNEFALCNMYYGGKSLVDPQFPLDIVDPSTLFCPPKEQTSEGFNVRVDNLYFFVTDQYLLEQFSPYGKISFCEVMRKPDLSNGSGRVTFSTREEAMEAIAAMEGKDIRGSSIKVTLEEENIPSSQVPLVDELEHGHAVKKPKVDNAVGSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DM19</accession>
    <name>A0A166DM19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_006251</name>
        <name evidence="8" type="ORF">DCAR_0207052</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Frigida family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DM19"/>
    <dbReference type="STRING" id="79200.A0A166DM19"/>
    <dbReference type="Gramene" id="KZN05414">
        <property type="protein sequence ID" value="KZN05414"/>
        <property type="gene ID" value="DCAR_006251"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206000"/>
    <dbReference type="OMA" id="VEMWKEH"/>
    <dbReference type="OrthoDB" id="1917867at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012474">
        <property type="entry name" value="Frigida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791:SF10">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4 5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="383"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN05414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG87820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="535" mass="58193" checksum="0CC9E4C89396CFD2" modified="2016-07-06" version="1">MATDYIINPDKIQTFFTQIEEHKNLLTSITDFHKTLATHFTSLSETLAQKSQNLDAQIESFHTQSKKSLELLENRENSIPEREASLVARINEQKGVSISDIESDNVGRNLGELLRMYFRRMDGSGLVKFLLAKRKESVVLRTELGSAVEEAVDVFALVLDVVEEFVGLKSEGGVGMADRRWACGLLVQVVVPLEEGKVGEVMVGSSLKERAMMVLEKWKGMLGGGGESGVGAGEATMFLQIVYGFGLKDRFEEKFLRSMVLEFAGRRDMAKIAAAVGFTGQKMIDIIDELVKSGKEVEAVYFATEFDLTEKYAPVSLLKSHLKNIRKNANSISKNGKYSAAAVDEANNSEMIATKAIIKCVEDHKLESQFPTDNLKKRITQLEKAKAEKKKSASSVSKPSNKRAHGGSSKGGGGVGTSSRPPKAGRISTTSPSFRQRNPSQIHQTPGATRYAAPYSYPNPSLYDVPGTPSYVSAYGGPRIKSPVGLQHAYAAPDVGVAGIRAGTSYAGQGSYGVQSNYAAYDYPPASAYPPTYPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EA44</accession>
    <name>A0A166EA44_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_007125</name>
        <name evidence="3" type="ORF">DCAR_0208044</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EA44"/>
    <dbReference type="STRING" id="79200.A0A166EA44"/>
    <dbReference type="Gramene" id="KZN06288">
        <property type="protein sequence ID" value="KZN06288"/>
        <property type="gene ID" value="DCAR_007125"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209520"/>
    <dbReference type="OMA" id="KSQWRAQ"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF463">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN06288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="14891" checksum="C05E3B5B2DD21CF6" modified="2016-07-06" version="1">MGANQSAVYDDSNDFSGVVMPRRFGRILSFSSSSKWKSHLQVTRKTNKLMVIHFTAAWCGPCRTMDPVIREFAGIYAGVEFIKIDVDELEDVAREYAVQALPAFVLIKKGKAVDKVVGAEKIALQKKIETYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GEY7</accession>
    <name>A0A166GEY7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0101485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GEY7"/>
    <dbReference type="Gramene" id="KZN08882">
        <property type="protein sequence ID" value="KZN08882"/>
        <property type="gene ID" value="DCAR_001538"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214004"/>
    <dbReference type="OMA" id="TEEWLIA"/>
    <dbReference type="OrthoDB" id="2019763at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010581">
        <property type="term" value="P:regulation of starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23160:SF19">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23160">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="753" mass="86574" checksum="4BDD650D220848F4" modified="2016-07-06" version="1">MALSTALQPNLPSKFCFVRLDRIQKRFAFVTHQRKRTCAVWIKSVLQEGNPGFSDSEATEPSITILERLYDQKQKLEKQTCEDLNLTQDLRSELGNLDCALQVAWAVLKKKEKDLREAETKVMLECNELNQAKEELDRREEELSAASLKHKTLEEELKQFNTDLTRQATEIEDLKLQVKKKDQDISVAQCALSLKEEEINKSVNELIRKSEVYANIEIELKYRNKLLNAANNVVKRQELEVQELRQGILVEEKEVGASLNLRKVENERLEVAEANLEKQTTAWLLAQEELKKLAYEASKNGREANKTVAEFKRVKKLLASVRSELLSTQKGLSSSREKRNEQQQVLEKQLIELEEQRSNVATYMTSLKDAQIEVDSERIMLRTAEAENHQLEVYLQLKKELINGLQVELNEEKSAVQHAIGEMYSLQEHLNKRVSDHEASVKLLQVKEEELVEGRLKIQHLSSEKRSLQLILEEKNLELSNTRIMLEDVNQEMNELSSFMDGREDQLHQATTILKETEEHAQKVQHELTDAKIRLSEAEIVVEQIAELTEDEDYDSQNLCSEMEHKFHDLREKPIDVFKWKKEQLETVLEITRESLRSKEMEVLAGQRAITIKDGELEMVLQRLDEREKELRMVKKALDSDVNHLQDLYTLTQGKFGGESVGDLAFEKLQLEAAQLEVEAATTTLGKLTEMSQQLINQAHLNMEVDYNIQAVTHEPGINANKESFSNVQAELAQFSALTDQLVKQAGIVDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YCN0</accession>
    <name>A0A175YCN0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">atpA</name>
        <name evidence="19" type="ORF">DCAR_0100109</name>
        <name evidence="20" type="ORF">DCAR_0C036259</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="20"/>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069416.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YCN0"/>
    <dbReference type="SMR" id="A0A175YCN0"/>
    <dbReference type="GeneID" id="88614231"/>
    <dbReference type="Gramene" id="KZM81207">
        <property type="protein sequence ID" value="KZM81207"/>
        <property type="gene ID" value="DCAR_032469"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614231"/>
    <dbReference type="OMA" id="WRISHIR"/>
    <dbReference type="OrthoDB" id="9805536at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Plastid Pltd"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18116">
        <property type="entry name" value="ATP-synt_F1_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1-ATPase_alpha_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.150.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000002">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="entry name" value="ATP synthase alpha/beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a_nt-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009884">
        <property type="entry name" value="PRK13343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0934">Plastid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="18">
        <location>
            <begin position="29"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="150"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase alpha subunit C-terminal" evidence="17">
        <location>
            <begin position="372"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG80964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH16700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="507" mass="55486" checksum="FD5994BBF08526CD" modified="2016-09-07" version="1">MVTIRADEISKIIRERIEEYNREVKIVNTGTVLQVGDGIARIHGLDEVMAGELVEFQEGTVGIALNLESTNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGRVVNALAKPIDGRGEISASEYRLIESPAPGIISRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTNFQERGAMEYTIVVAETADSPATLQYLAPYTGAALAEFFMYRKRHTLIIYDDPSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLGSLLGEGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVGSAAQIKAMKQVAGKLKLELAQFAELEAFAQFASDLDKATQNQLARGQRLRELLKQSQSAPLAVEEQIMTIYTGTNGYLDSLEIGQVRKFLVELRTYLKTNKPQFQEIISSTKIFTEEAEALLKEAIQEQMERFILQEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YEC8</accession>
    <name>A0A175YEC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_029609</name>
        <name evidence="11" type="ORF">DCAR_0934113</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YEC8"/>
    <dbReference type="STRING" id="79200.A0A175YEC8"/>
    <dbReference type="Gramene" id="KZM81996">
        <property type="protein sequence ID" value="KZM81996"/>
        <property type="gene ID" value="DCAR_029609"/>
    </dbReference>
    <dbReference type="OMA" id="YHHKEKE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF50">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="405"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM81996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH14593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="558" mass="62278" checksum="0647A5AEF96B6A1F" modified="2016-09-07" version="1">MTSTMNYMKSVLYDAVTNDDNNALAAMQASDLEDQRTPTNSTILHLACQYGSINCVQHILMNHSSLLLKTNSRGETALHLAAKQGQLDVVQELVSTATASMTVQNLMRSADEDLETALHGAVRYNRRAVVELLVVTDPSYPHPQNKYKETPLYLASIRYYTDIITTILVNCNWPNIISGSAGGGTDLHAICGGPEGRTALHAVVLDYRNHGGHECVQRLLDWNIGLLKEVDDYGWTVFHYAAHNDLHTIVELIIGLLVADEDKYVAYHKDKMYGRTPLHIAAYTGNVDVMKTFVKHFPDCWEITDGSGRNILHIAVEEDRKDRRPAEWEVDWEVLDSNNYTPLDVLHLEQETHTLANQLLVRTSLIKANVRKHWWLWRTLREPNADSGKRIVKHTEITNNIEIEEHRKAINTHMVVSALIATVAFSVLFNVPGGFDGSKGSPVLLRKTDFAYFIILDALALMLSVASLLLYFFTSSNKDASQVKRIVRNTAALNFLAICSMMVAFVEGTSVMLADKSTLGAGVNLLILFFVIGATPVYIYNLRGEVRSRTLLPASNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIS0</accession>
    <name>A0A175YIS0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">aminocyclopropanecarboxylate oxidase</fullName>
            <ecNumber evidence="9">1.14.17.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ethylene-forming enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0729188</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 = ethene + L-dehydroascorbate + hydrogen cyanide + CO2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:23640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18153"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.14.17.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.17.4" evidence="9"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YIS0"/>
    <dbReference type="Gramene" id="KZM83317">
        <property type="protein sequence ID" value="KZM83317"/>
        <property type="gene ID" value="DCAR_030886"/>
    </dbReference>
    <dbReference type="OMA" id="NPEMIKG"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009815">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071398">
        <property type="term" value="P:cellular response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000002">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="13">
        <location>
            <begin position="153"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH09730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="311" mass="35533" checksum="10546FCE2F2D8D34" modified="2016-09-07" version="1">MESFPIINMEKLNGEERAATMDRIKDACENWGFFEVLNHGISIELMDTVERLTKEHYKKSMEQRFKEMVEKKGLETVQNEIDDLDWESTFFLSHLPQSNMADIPDLEDEYRKAMKEFAKGIEKLAEELLDLLCENLGLEKGYLKKAFCGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDQVSGLQLLKDGEWIDVPPMRHSIVINLGDQLEVITNGKYKSVMHRVIAQTDGNRMSLASFYNPGGDAVIYPEPKLLEKEEQNGQVYPKFVFQDYMKLYAGLKFQAKEPRFEAMKAMEITTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIY7</accession>
    <name>A0A175YIY7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0830398</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH10921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YIY7"/>
    <dbReference type="Gramene" id="KZM83686">
        <property type="protein sequence ID" value="KZM83686"/>
        <property type="gene ID" value="DCAR_028892"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM83688">
        <property type="protein sequence ID" value="KZM83688"/>
        <property type="gene ID" value="DCAR_028890"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199138"/>
    <dbReference type="OMA" id="WRREHLL"/>
    <dbReference type="OrthoDB" id="1924823at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF57">
        <property type="entry name" value="CASP-LIKE PROTEIN 4B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="23059" checksum="3659A14476678874" modified="2016-09-07" version="1">MANVEDSTNSPENSETLPPSAPPLPPPAPPVVANDVEQGETPSASVTKTPEIRRSWRREDLYKNASLALRVVGLLFSFLALIIMATNKHDGANFVDFDEYSYLLAVAVISTVYTGGQVYLQVHELATGVQTFSHKKLSLCSFIGDQIAAYLLISAGSAAVPITNSLRELEDDYSSGGSLFTDSSSAAIAMAFIAFLFLALSALVSGYKLSRQSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YN58</accession>
    <name>A0A175YN58_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0831553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YN58"/>
    <dbReference type="Gramene" id="KZM84680">
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        <property type="gene ID" value="DCAR_027898"/>
    </dbReference>
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    <dbReference type="OMA" id="HTECLAY"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37242">
        <property type="entry name" value="OS09G0569450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS09G0569450 PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="102" mass="11347" checksum="52D519F5B8CC2722" modified="2016-09-07" version="1">MAEVPDDPPPQPAATSPPPATLDPPPPPPSPPRFDPSRMIGIIRRKALVKDLAAAYHAECLAYCQELLELQRKCEESIIDVKVAEESRKEAMRPPKRLKKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3W4</accession>
    <name>A0AAF0W3W4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="ORF">DCAR_0101913</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
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    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3W4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016004">
        <property type="term" value="F:phospholipase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
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        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
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        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="48"/>
        </location>
        <ligand>
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        </ligand>
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        </ligand>
    </feature>
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    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="169" mass="19246" checksum="A832EE1703F2C994" modified="2024-05-29" version="1">MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGMISLLTAICSIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3Y2</accession>
    <name>A0AAF0W3Y2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0101035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="134"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="192" mass="22411" checksum="910432ED8C790B87" modified="2024-05-29" version="1">MSQCYILAAMSGVLQHQHQAMELFGDQNRAAMQVAMKALMNTQMVEGTPIDIILLSLTKSFEQFRLNDNMNKRQYSLAELLTELQAAEGLFHQSVQVNVAEKGTQDPVEAQSWLKEMEKAFRLVVVTDEKKVEYASYFLKGETNYWWESVRALEEGEVIAWDRFKKIFLDKYFPRYVQTQMELKFFELKQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4U6</accession>
    <name>A0AAF0W4U6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </reference>
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</entry>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
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    </reference>
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        <text evidence="3 8">Homodimer and heterodimers.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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    </comment>
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        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    </dbReference>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF04535"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="218" mass="24494" checksum="1E0EB6164673CCAA" modified="2024-05-29" version="1">MEKKQQNFRSQNPSPPPYESSISSFSPSSFSRDGEGPEHSLHPSPAPQKEISRKQSTKRTGDDQVLKKVNFWFRVFGFLFCLISFSIMAADRDKGWTLDSFHRYIEFRYCLTVNVIGFIYSALQGFDLAYQLFSSDGNKLLVPASHLRNIVDFASDQILAYLLMSSSSSAATRVEDWRSNWGKDKFPNMANASVAVSFMAFVSLASSSLISGYTICTL</sequence>
</entry>
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    <accession>A0AAF0W808</accession>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ashrafi H."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG83378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="102" mass="11158" checksum="ED8CFC72207F3315" modified="2024-05-29" version="1">MESVTKMVSQRPVVIFSKTSCCISHTVKSLLQDFGVSPVVYELDEIPRGRDIEVALARLGHNPVVPAVFIGGELVGGANEVMSLHLQRNLRPMLTRAGAMWV</sequence>
</entry>
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            <fullName evidence="4">Exonuclease 1</fullName>
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    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Putative 5'-&gt;3' double-stranded DNA exonuclease which may also contain a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. May be involved in DNA mismatch repair (MMR).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8V7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0228">DNA excision</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="18">
        <location>
            <begin position="138"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG85032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="788" mass="88082" checksum="73ADA81DC9F71C7E" modified="2024-05-29" version="1">MGIQGLLPLLKSIMVPIHIKDLEGCSVAVDTYSWLHKGALSCSKELCTGQPTTKHIAYCMHRVNMLRHHGIKPILVFDGGHLPMKGEQENKRARSRKENFERALEHESGGNRSAAYECYSKAVDITPSIAYDLIQVLKQENVAYVVAPYEADAQMTFLAVNKLVDAVITEDSDLIPFGCPRIIYKMDKFGQGVEFHSSLLQQNKDLNLTGFTKQMFLEMCILSGCDYLQSLPGMGLKKAHGLVKKFKSYDKVIKHLKYSNIAVPPLYEEFFRKALLTFQHQRVYDPIKADIVHLSDISYNDGEDIDFLAKGIATGELDPFTKLPFQGKCVTTVSVLDGTGQPYNTKPEGERKKIDLPVQKNLLTNYFCFASLEAKRKFRVPRVTSNNPEMSIKVNTSEDNNETALSYNMDCSRPALAYSGELENSDPTKDSVLNDVAPNSSLSSELAHENKDGELLLKDSYSAARSVSSIHKYEYKSTFDRTENEPIFEQGHMKSDTVMNKMRVEPKNVIVRSSYFQKNSAKGNGLENKSGEMFVKDSNHTARSVSTIQECQFKSALDRSEGETTLEDRDTILQSPIKSVITADRENTNETMLIDVAASDSQATATPVHEFKSAKDAALCKTGAESRKVVVRSSYFKHKAEKENDTIHDAGNKHYTGTNSKRKLDDNVQIDITTRKCLRANESSPIQSTCTLDPEDSMETKNEQDKFGCNISHIDRYSDIAGKSVDKFAALISSFRYTAGSRPSGLRAPLKDVRNTCTNSKTKDVADLSKFAYVPTKRLTLSASRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCB1</accession>
    <name>A0AAF0WCB1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EGF-like calcium-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0104682</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCB1"/>
    <dbReference type="KEGG" id="dcr:108203310"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000001">
        <property type="entry name" value="Vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF4">
        <property type="entry name" value="VACUOLAR-SORTING RECEPTOR 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042118053" description="EGF-like calcium-binding domain-containing protein" evidence="16">
        <location>
            <begin position="20"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="566"/>
            <end position="589"/>
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            <begin position="517"/>
            <end position="559"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="630" mass="70201" checksum="0B64B7B220959E20" modified="2024-05-29" version="1">MMRNSMAMLFVAVVGVVIACYVVEGRFVVEKESISVLSPSDLRYTRHDASIGNFGVPDYGGSMVGSVVYPQKGSNGCSSFDGDKPFKSKSSRPIILLLDRGECYFALKAWNGQEAGAAAVLVADDRDEPLITMDSPQESSDANGYIDKIGIPSALIEKSFGDTLREAIQKGKEDVVIRLDWRESMPHPDQRVEYEFWTNSNDECGIRCDEQMKFVKDFKGHAQILEKGGYTMFTPHYITWYCPQPFILSDQCKSQCINNGRYCAPDPENDFGKGYQGRDVVIENLRQLCVHRVANESHRSWVWWDYVTDFHIRCSMKQMRYSKECAESVMESLDLPIAKIRSCMGDPEADVENKVLKIEQDRQVGEGSRGDITILPTLVINNVQYRGKLERTAILKAICAGFQETADPPICLSGALETNECLERNGGCWSDVRSNITACKDTFRGRVCECPVANGVQYRGDGYKSCEAFGPGRCAISNGGCWSETKNGQTFSACSDSDLSGCRCPQGFRGDGHKCADINECTEDHVCRCDGCSCKNSWGGYECKCKGDKLYIKEHDTCIAKNASKFGWFLTLLVLGTTAAAATAGYLFYKYRLRTYMDSEIMAIMSQYMPLDGQHNQVTQHEAEPLQQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDW6</accession>
    <name>A0AAF0WDW6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0205520</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
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            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG86318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WFR2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0207564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFR2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF185">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="9"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG88329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="229" mass="26201" checksum="60764B684A2CD903" modified="2024-05-29" version="1">MKLKGGPNKACAACKFQRRRCSSDCSLSPFFPADQPKIFNDVHRLFGVSKVIKILKKMRDHDQKVDAMKSIIFESYIREKYPVHGCFGIISQLDQELHQATEELHYVRAQLSMLKENNSQSSQIMNSYDVASENNDNGAFYSDQSGDQTKSFMNLNSYYSDGMSDDNKLLEIQTQFGDSNTFALNHSQVGEASDQDYDIMNLYAIDDQSSKEACESRYEYLSFFFLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WK15</accession>
    <name>A0AAF0WK15_DAUCS</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0310604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </evidence>
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</entry>
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    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="84"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="316"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG90986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="993" mass="111168" checksum="16AFEDB6333A734B" modified="2024-05-29" version="1">MEGSPSMGKRKVSEENLGDVAKKEEPVLKKANVTRTCVHEVAVPSGYTAVKDESVHGTLSDPVFTGTMAKTYQFTLDPFQSVSVACLERNESVLVSAHTSAGKTAVAEYAIAMSFRDKQRVIYTSPLKALSNQKYRELSQEFTDVGLMTGDVTLSPNASCLVMTTEILRGMLYRGSEVLKEVAWVIFDEIHYMKDRERGVVWEESIIFLPPAIKMVFLSATMSNATEFAEWICNIHKQPCHVVYTDFRPTPLQHYVFPVGGSGLYLVVDENESFREDNFVKLQDTFAKPKQIDGNKSGGKANGRIAKGGNGSGGSDIYKIVKMIMERKFQPVIIFSFSRRECEQHAMSMSKLDFNSQEEKDIVEQVFRNAILCLNEEDRNLPAIELMLPLLQRGIAVHHSGLLPIIKELVELLFQEGLVKALFATETFAMGLNMPAKTVVFTSVKKWDGDSHRYIGSGEYIQMSGRAGRRGKDDRGICIIMVDEQMEMNTLKDMVLGKPAPLVSTFRLSYYSILNLMSRASGQFTAEHVIKNSFHQFQYEKALPDIGNKVTELEKEAALLDASGEAQVAEYHNLKLEIGHLEKKMMAEITRPERVLYYLLPGRLVKVREGGTDWGWGVVVNVVKSPSAAPGTLPSALSSSRGCNYIVDTLLHCSIGSNENGARPKPCLPRPGEKGEMHVVPVQLPLISTLSKIRISVPSDLRPVEVRQSILLAVQELGKRFPQGLPKLNPVKDMGIEEPELVELVNQIETIEKQMMSHPLFKSQDENQIKCFQRKAEVNHEIQQLKTKMRDSQLQKFRNELKNRSRVLKKLGHIDADGVVQLKGRAACLIDTGDELLVTELMFNGTFNNLDHHQIAALASCFIPGDRSNEQIQLRAELNKPLQQLQDSARRIAEIQHECKLEVVVDEYVEAAVRPFLMDVIYCWSKGATFAEVIQMTDIFEGSIIRLARRLDEFLNQLRAAAHAVGEGDLESKFAAASESLRRGIMFANSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLW2</accession>
    <name>A0AAF0WLW2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLW2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF515">
        <property type="entry name" value="GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28102" checksum="E556407828E1B19E" modified="2024-05-29" version="1">MEECKSVSTPAEASIKLRIDSTRESVNPTLFKSLVGSLRYLTFTRPDIMYAVGLVSRYMEKPKQDHFMAAKRILRYIKGTLNYGLFYTHSQNSKLVGYSDSDYGGDLDDGKSTSGYAFHIGSAIFSWSSKKQQTVALSTCEAEYIAAAACACQAMWLGYILGELNLAKEDPVTIYVDNKSAISLAKNPVSHSRSKHINIKYHFLREQVNDKNVELVHCRTEENLADIFTKPLKPDVFNKMITKLGMQSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM90</accession>
    <name>A0AAF0WM90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORF/ORRM1/DAG-like MORF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM90"/>
    <dbReference type="KEGG" id="dcr:108211016"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900865">
        <property type="term" value="P:chloroplast RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000001">
        <property type="entry name" value="Multiple organellar RNA editing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039206">
        <property type="entry name" value="MORF/ORRM1/DAG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054059">
        <property type="entry name" value="MORF/ORRM1/DAG-like_MORF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 2, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346:SF3">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 9, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21864">
        <property type="entry name" value="MORF_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="MORF/ORRM1/DAG-like MORF" evidence="3">
        <location>
            <begin position="85"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="228" mass="25721" checksum="887A1D09BF3D059B" modified="2024-05-29" version="1">MATLNLSPLSKTLINPTPLKPSTPLSCLSLSYPLQSLSFSLTQNLKPTRNSSRTLIRAAVDGDYSAKRSNSSEPRETIMLPGCDYNHWLIVMEFPKDPAPTREQMIDTYLETLATVLGSMEEAKKNMYAFSTTTYTGFQCTVSEEVSEKFKGLPGVLWVLPDSYIDVKNKDYGGDKYVNGEIIPCTYPTYQPKQQRRSKYESKRYVRQRDGPPPERRKPKQATESPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN46</accession>
    <name>A0AAF0WN46_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase Pif1-like 2B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0312208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN46"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23274">
        <property type="entry name" value="DNA HELICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23274:SF51">
        <property type="entry name" value="OS03G0423850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="29640" checksum="E59C5F534E2F928A" modified="2024-05-29" version="1">MLNLPYIILLHIPPYKITYTIIPFQVQVTYSGDPVKAIVDHIYKDLDEGHGSLEYLRDRAILTPLNEYVDKINREVSDTICKGSSTNEGDEILYPAEYLNSLKFSGVPNHELELKVGVPIMLLRNLNPKKGLCNGTRLIVTQCCPFVIEGMIITGNKTGEKTYIPRICMSPADKTMPFVLKRKQFPISVCYAMTINKSQGQTVKNVGLYLPKPVFCHGQLYVAVSRVTSPSGLKIVSVNEEDMCDGYTKNVVYQEIFNNIVPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNB9</accession>
    <name>A0AAF0WNB9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0311552</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNB9"/>
    <dbReference type="KEGG" id="dcr:108212337"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="11">
        <location>
            <begin position="103"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="12">
        <location>
            <begin position="352"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="495"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG92289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="91332" checksum="3415B2C6B0B05E8B" modified="2024-05-29" version="1">MSNDTTLNLLVSDLLSYVRTSVDVADYSDLSLLVVGDRLASEVAQIYKASMQDYDFRDITSKFYFDRKKFEEEPEYAESSSDLPDSEFKREVFVGYQAEASSLLQQLVSITKKRLQVISIVGMAGLGKTTLARRLYNDPYVVSYFYVRAWVTCSQIYHKRNLLLGILRSVAEVTDDVYQMDDNMLAHHLYRALMGRRYLIVIDDIWSREAWDDFKNCFPDDKNGSRIMLTTRLKVIASHAQSDGDPLCLRFLTEDESFELFRRKTFIRGAYFSDLSSIGHGITKKCHGLPLAIVVIAGLLKDNLDRDWWIHVAKSVRSYIVTDENQYIETLALSYNHLPQHLRPCFLSFGAFPEDYGISVRKLIYLWIAEGFIHLDGTQKTLEDVAEDHLTDLISRSLVVVGKKGSNGAMKTCYIHDLLRELCVRKAKEENFSLNISNYNKHSYPCSHSSNDPCRESQLLLSPNVPGIPSICLCYSTELSVKFFDGVSIVWETSKVIRALDLSSLKFSVFPIALVQLVHLRYLELRFRTGNLPESICRLQELQTLIMTSRMNMVVPKYIWKMINLRHLCIKTGENPIDFSNVEEEPSFLENLQSLSLVSPASPCQHILAKTKNLRKLGLCGTLTTRSGEFKCPDLGLLMHLETLKLLNTTPFCKAGRLSNSIAFPGSLKKLTVSNTYLDWKEAWVFEVMPNLEVLKLKSHAFFGDCWEPSPEAFPCLKFLKLEELDIVTWTASRNHFPVLEHLQVIGCLLLMEIPEDFGNICTLEWIEVSGCSNAVTSSVREIQKEQKSYGNDLLHIRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNN5</accession>
    <name>A0AAF0WNN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310548</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Clathrin-coated vesicle</location>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
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        <property type="entry name" value="ANTH_N_plant"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS06G0175500 PROTEIN"/>
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    </evidence>
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        <source>
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        </source>
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</entry>
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    <reference evidence="1" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Ribosomal protein L34Ae</fullName>
        </recommendedName>
    </protein>
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    <reference evidence="2" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF1666"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46741:SF4">
        <property type="entry name" value="FINGER FYVE DOMAIN PROTEIN, PUTATIVE (DUF1666)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS09G0413600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07891">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="220"/>
            <end position="256"/>
        </location>
    </feature>
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        <location>
            <begin position="284"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
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        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG93995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="84582" checksum="4E23E7E1F3F78251" modified="2024-05-29" version="1">MDLLGVFNKHFRGEIMLRFYTSFCVLSWSWIFDVFGFLRNYISRKLNSVVFSQSVHDDDDDDEDDNDEFFRESASDEYVEKEFSEFSFGFKKYDQDFRETECSVSSVSNYQFMSSRDVSGFVQVPETMSFSVEESFEGFIEKDLILFNGVTRNKDFVEEDLEGLRKEESDQFIDLKVSDLRKDQQVTSQVEFVQQDQGEDSLEESGLKEKEELQSEIDELSQNLEEGTKGSSLESSVDSREIISTRNSGSSDEENLTYNKFSFNDIDDDDEFIELEPRLHHSVDGICNESPADGLDKESPHDNSHNNETPPLNGLENSEERCSEESKSMEWESDEDDEEDDILLEHKYLIEQMKMEAKHSRNGGLPTILEESETTTKIPDDLKPLQLDESFDHSDRMEEIQKFYKSYSEKMRKMDILNRQTMHAISFIQLKEPVKRISSQKSAVSAMKSIFLNNFNQGKLRKIYADQTLKKSMMDLHRDSERVYVGQICISWEILHWQYLKAQELQEHDSQDCHTYNQVAGEFQQFQVLLHRFIEDELFQGPRVQHYVRKRMAILSLLQVPIIKDDRFKNKPVKEEDAISIAVLKQIIEESMRVFWEYLHADKHEDSYVLKRFHSTKLNHQDAAAHIELLMDIKSKLQKKEKRLKEILRSSNCIVKKIQKHQGGRVKHDLLICQVELRLVSRVLHMSRCTTDQLIWCLTKLNKINIVNRKVSVEPSFLLFPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRW4</accession>
    <name>A0AAF0WRW4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0313529</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRW4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17971">
        <property type="entry name" value="DEXHc_DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21691">
        <property type="entry name" value="GH2-like_DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05684">
        <property type="entry name" value="S1_DHX8_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000061">
        <property type="entry name" value="ATP-dependent RNA helicase DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000101">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000191">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044762">
        <property type="entry name" value="DHX8/Prp22_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049588">
        <property type="entry name" value="DHX8_GH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049621">
        <property type="entry name" value="S1_DHX8_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="557"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="738"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="327"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG94236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1200" mass="136254" checksum="8F372170009EF35A" modified="2024-05-29" version="1">MASEVKDEGLKKLEYLSLVSKVCTELESHLGFGDKVLAEFITELGRDCESVDVFDAKLKENGAEMPDYFVRTLLTIIHAILPPSVGKSKKGGEGKKGEEGGDSKFPALNISDSRERVKELEREIALEAKEKARKDGGDDERRYRDRGGEDRGGGRGRDREGGGDERDRRGDRGERRRDDRGDFRGKERHRDRGDRNRRDGYDEDGEDDRGDFRGKERYKERNDRNRRDGYDEEGERGRDKSMSGEPELYQVYKGRVQRVMDTGCFVQLNELRGKEGLVHVSQMATRRISNAKDVVKRDQEVYVKVISISNQKLSLSMRDVDQNTGEDLLPLKKSSEDSRMNPSGSNNGGPTTRTGLSGIRITDDDAPLPSRRPLKRMSSPERWEAQQLIASGVLSVKEYPMFDDETDGLLYEEEGADEELEIEMNEDEPAFLNGQTRYSMDMSPVKIFKNPEGSLSRAAALQSALIKERREVREQQQRTMLDSIPKDLNRPWEDPMPETGERHLAQELRGVGLSAYDMPEWKKDAFGSALTFGQRSKLSIQDQRKSLPIYKLKKELVQAVHDNQVLVVIGETGSGKTTQVTQYLAEAGYTTSGKIGCTQPRRVAAMSVAKRVAEEFGCRLGEEVGYAIRFEDCTGPDTVIKYMTDGMLMREILIDENLSQYSIIMLDEAHERTIHTDILFGLLKRLVKRRPDLRLIVTSATLDAEKFSGYFFSCNIFTIPGRTFPVEILYTKQPESDYLDAALITVMQIHLTEPEGDILVFLTGQEEIDHACQCLYERMKGLGKNIPELIILPVYSALPSEMQSRIFDPAPPGKRKVVVATNIAEASLTIDGIYYVIDPGFAKQNVYNPKQGLDSLVITPISQASAKQRAGRAGRTGPGKCYRLYTESAFHNEMSPTSIPEIQRINLGVTTLNMKAMGINDLLSFDFMDPPSPQALISAMEQLYSLGALDEEGLLTKMGRKMAEFPMDPPLSKMLLASVDLGCSDEILTIIAMIQTGNVYYRPREKQAQADQKRAKFFQPEGDHLTLLAVYEAWKAKNFSGPWCFENFVQSRSLRRAQDVRKQLLSIMDRYKLDIVSAGKNYMKIRKAITAGFFFHAARKDPQEGYRTLVENQPVYIHPSSALFQRQPDWVIYHELVMTTKEYMREVTVVDPKWLVELAPRFFKVADPTKMSKRKRQERIEPLYDRYHEPNSWRLSKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT19</accession>
    <name>A0AAF0WT19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0414897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RNase_H-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="34"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="30649" checksum="DB07734D1DAAFCBC" modified="2024-05-29" version="1">MQHVNPPGFKVPNPGSLKINTHGVWSSIPSDIGNHSGIGAVYRDSNGTLSHVTVGTIPFLSRLGTQLWAIYAPLRRAMIKGYSSVILETDNYQAYRVIRDFQHGAPAEVFDIVSQIDILLKDRRWTCVIAYVYPARNHVARFTARLGKDVADRLYTLNRMIGPLEELLDWDMGLGVDHPDFMDVVLPDEAPDPVNFDVALGIADQVDGLALGQLHFQGGMAPAAMQGSAPASQPMENGGMHANAATGMVDEDAVGPQEETIQPTLRDTGFLFAGDSPLEDVD</sequence>
</entry>
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    <name>A0AAF0WT86_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0313717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WT86"/>
    <dbReference type="KEGG" id="dcr:108211196"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="entry name" value="Uncharacterized protein At5g02240"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="62"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="304" mass="32927" checksum="DB4D78F65769580A" modified="2024-05-29" version="1">MASLSLLSTPLTHQTVTYTSSCKYNFPSTGVFRTSTRKGFKRHTSVVTAMSDLAQSTVLVTGAAGRTGQIVYKKLKEKPDQYIGRGLVRTEESKEKIGGADDVFVGDIRDADSIVPAVQGIDFLVILTSGVPKMKPGFDPSKGGRPEFYFEEGAYPEQVDWIGQKNQIDAAIAAGVKQIVLVGSMGGTDLSNPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKEGGIRELLVGKDDELLQTDTRTISRADVAEVCIQALQFEEAKYKAFDLASKPEGTGTPTKDFKALFSNITTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WU48</accession>
    <name>A0AAF0WU48_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0314168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108215111"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
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        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042068867" description="Protein-serine/threonine phosphatase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="66"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="589"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG94871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1073" mass="121039" checksum="CE6D2B9DF36323A9" modified="2024-05-29" version="1">MYTHKLCVYILGFIVHLFVIVNGESSTCLTVYNEGGAPAVFQSPKCPRWKLPEYGSSRRSRSPVATCQVALHQGRRKAQEDRLVCNLDVRIPFPGPAGITEVAVGVAAIFDGHNGAEASEMASKLLIEYFMLHIYFILDTTFSLLSEKAIGRLPNKGEDSQLHNLNFGRFKLTLDAILDGSVHLEILKESLLRAIRDIDATFSKEASRNKFGSGSTATVILIADDHILIANVGDSKAFLCSEIFQSPPEAKATLLRLYRQKRREGAKPSIRNFSGFTSAASSGLVHFSAVELTRDHHPDREDERSRVESAGGYVLEWAGVSRVNGELAVSRAIGDLPFKSYGVTSVPEVMGWRPLHANDTYLVAASDGVFEKLSPQEVCDLLWELHNQGPMRSEVSPACPYSLADCIVSTAFDKGSTDNMAAIVVPFRSTGFSKTLLKDRLHEQEKFELPALGQQKYIHDKSQLEHSHPSIRKFDRLLVEGKDGGFETFYLSENLDEYDDTVWSQRDDQEHMHDQSQALPYPLNSYCGGSLDLYSDQNMCLRYGMTNDGHRDQCPEGIASFLSLLESIPIYNVGSDYGSNEHTVPYSRYILKKRFDRGSYGEVWLAFHWNSSIGTNASRWRFPSSTFDPIPCDRDVDGSFTSDCGAGIPDDIKFILKRIMVERGNAVYLSGLREKHFGEVFYNASTVLGSAIIEDSVLTESESNLFDMLITNDSAAQETEFTWSTKNTFPEKDSQQKDAFEEGLNHIARYVESFESRSNEIWLVFRHEGISLSKLMYTVEEVENDSADEQVTRVQLLHPSEWWHWLKTTEAGQEEMRNLIRQLLMALKSCHDRNITHRDIKPENMVICFEDLNSGRCLKGTPSGDQNYTTKMRIIDFGSAMDEYTVNHLYGSLGPSRAEQTYEYTPPEALLNASWYRAPTNIVHKYDMWSVGVVFLELILGSPNVFRISSLSRILLDQHIEGWNESLKDLAYKLRSLMEMCILVVGNSSKHHRTCGANNMKSELPASWKCSEDFFAQQIKNYDPLKIGFPNVLALRLVRQLLLWDPEDRLSVDEALRHPYFSHNAQGHIQGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUW3</accession>
    <name>A0AAF0WUW3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUW3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033045">
        <property type="term" value="P:regulation of sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032245">
        <property type="entry name" value="RMI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962:SF1">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2 RMI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16100">
        <property type="entry name" value="RMI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="123" mass="13824" checksum="B3AC2AF191FD9D8E" modified="2024-05-29" version="1">MDYKLAALKLTCVQLKAARQIQTNSQSRGILVTSPSDDADGRYVLDDGTGVIELVLVGDISNHKFETGMYLMVVGVFLVRDGDIPLLKVHKAVDLSAFPNREVMWYLEVLEAYKLFYQPPMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVR4</accession>
    <name>A0AAF0WVR4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0415130</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVR4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF8">
        <property type="entry name" value="CYTOCHROME P450 82C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="59584" checksum="CB9AE1E65C617964" modified="2024-05-29" version="1">MAIHIQLQDIILFFLGLFVGTISWRFFSTYVFRKNSCPGPPEPAGKWPLIGHLHLLGANKILHHTLGDMADRYGPIFCLNLGTKRTLVVTSWEVAKECFTAQDRVFATRPKSVVGKVVGYDSRVMIFQEYGSYWRQIRKLAMIELLSNRRLEMLKHVRESEVNLFIKELYEQWSRNGNGSKVVVEMKEKFGDLTTNFVVRTVAGKIYSGTGVQGNEESRRFQKAMADFMHLAGLFMVSDALPLLGWIDTLRGYRGEMKKSAEEIDLVLGSWLKEHQQKRNNISINRLDEDFIDVMLSAMEGNQFPDIDTDTAIKGTCLSLILGGYDTTSATLMWALSLMLNNRHVLKKAQDEMDKYVGRDRQVKESDVKDLTYLQAIVKETLRLYPAAPLSPQHEAMEDCTVAGYEIPAGTRLVVNLWKIHRDPRVWSDPLEFQPERFLQKHVNVDIWGQNFELLPFGSGRRSCPGITFAMQVLHLTLAQLLHAFELGTVLDSNIDMTESPGITNPKATPLEVTLTPRLPPEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWR2</accession>
    <name>A0AAF0WWR2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0415444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X1L9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042885">
        <property type="entry name" value="HIPP47/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932:SF12">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG99652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10233" checksum="EB2D254ED819D7EE" modified="2024-05-29" version="1">MRNKYSSYEDLAEQKIVIKVRSHCKKCRSKALAMAATESGVISVALEGEKKDQVVVIGDGVDAAGLTSSMRKKLGYASLELVEEIVRDDSVKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X385</accession>
    <name>A0AAF0X385_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X385"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="2">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="209" mass="23964" checksum="BEC8CBDF6039A261" modified="2024-05-29" version="1">MQVKKLLRQGCEAYLAHVVDLNAESPSIERIPVVNEFRDVFPNELPGLPVDREIEFAIDLAPSTEPVSRAPYQVQFLGHVVNNEGIKVDPAKIESIMNWERPKSPTEVRSFMGLAGYYRRFVKYFEKIASPLTKLTRKNEKYEWNDKCEESFQELKKRLVTAPILTLPDEQGNFMIYSDASHKGLGCVLIQHGKVIAYASRQLKTHEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X414</accession>
    <name>A0AAF0X414_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0520413</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X414"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="11"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="13">Leaf</tissue>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="13">Leaf</tissue>
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        <text evidence="9">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
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        <source>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH11433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF75">
        <property type="entry name" value="RIBONUCLEASE H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exo_endo_phos"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RVT_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="167"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="912"/>
            <end position="1190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH11433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1455" mass="166130" checksum="24583FDA77711BC5" modified="2024-05-29" version="1">MDPLLHFLRAYEHLIHPNSRVEALQGKREAVRMALNQIHYGSIELARLELKKGSPKHYASTEQSTKGKGEFDTRKTLLHHLQVAKNFIKTFQHLVEPSTLKDAMLGNDKAISLALGQIHHKTLPSKADGPTSQTYKEALLTPSKVATPKQIISPKARVDRNTIKGKVSIFFTGFDASTHARDLWQMFKKEGAIKDIILPRRKDKFGNKFGFVIVANFQEAERVIKALNGRQNGEAKLYLALAKNSRLSMEPVKLDIHELLHVELSSCVLLITAKRETIANVDMIVRGLGFKGVIIRGIAAKEIDLIPPRQAWVEIRGLPIQGWTEANYRQIIKPWGEIIHFGRTIDEDEFYVTPKVLIDTMQIENINTKITSATPRYDYNDIQDAEEEEVQNKERNIVVAHLAQEQALPLLRGPQTQIRGELPEVTILHTSNWIPREVESSISLPKSTSESELSEVNDDLYAHHPEYGPQTSDILKELKNLKVQVKRGRPRKYNRNQLNKHFKLPRRRKLRGEGLKQTTHFFLNANYDEAEAIFETGSLMGLLPLNSKENSMKFIKENLKDFAIVNVYAPQAFDSKKAVWKALDNEMSNTTESFTVILGDFNTVRDPAERINTKFKIKDNIDFNQFIASTEMVEVCMRNSYYTWFGPSNKCSKLDRIMVSKDWLTLGNWLVIDLDRYTSDHRPLCLTISKDDWGPKPYKFFNYLLEEQNFVEVLKQAWTQSNARNFISKFRNLRGVAKQWSAKHFGDLDEREVEGMDQVDIKEEMDKLLQIKVSMLCQKSRLNWELKGERNTRFFHRALARRQHRNQITALSVDGVDMCTPHVIKEALHSYFHQLLTETTTAKIFDLPDKMLAELKIEEREHLIKDFTLQEIHEALLATDATKSPGSDGINAGVLRSIWPVMKDEILVFFKEFHTTSHIPKGYNASFIALVPKVKVPKHPTHFRPISLMNSVMKLLSKVMARRLKEVMHVLVSPTQSAFIQGRQITDSILLASEVISALKDKKSSGLVLKIDFEKAFDKIRWNFVFEVLRQMNFDSKWIDWISSIFNSSSISVLVNGSPSSEFSPTRGLRQGDPLSPLLFNLVGEVLSAMLNRASELKIINGISLPNCKELITHLQFADDVVLFISQDIDSIMGIKRVLQCFQVISGLKINFSKSSLYGYGGSEQNLVEWAALLGCEVGKGELKYLGATLGASPTRLKYWDPLLAKVQARVQSYDSSTLSIAGRTVLLKAVIDSIPVYWFSLFKIPSAIINKIEKVRRRFLWGGKAGIGAKRTFHSLSWNKVCSPKRCGGLGLVPIRHRNEVLLAKWVWRAYNERDRYWNKLLGERYGRQWNYDLSQVATGTCSPIVKSIVSTHLWWSLHAIRIRDSNFSLLRLLSTRVFPAARGIWHLVVAAALWSLWLARNELIFTKTKIKKDALIELVHIRISKWGRACVKELIARYMSKPICTTSDNSTCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPP1</accession>
    <name>A0AAF0XPP1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0831170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF314">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF094"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ETHRSPELEMNT"/>
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    </dbReference>
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        <property type="entry name" value="DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="165" mass="18499" checksum="E450398D5051829C" modified="2024-05-29" version="1">MPLFEVLTKATSAEDECSQEELVAAYRGVRRRSWGKFAAEIRDSTRNGVRVWLGTFDTAEEAALAYDQAAFAMRGTGAVLNYPAQYVYQSLIEINYDFGAETSPVLALKKMHSSKKKTKKNKKANEETKFAHQDGLNNNVVVLEDLGADYLEELLTLSEASQYSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQ69</accession>
    <name>A0AAF0XQ69_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF223 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0831602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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    </evidence>
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</entry>
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                <person name="Iovene M."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <location>
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        <location>
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        <source>
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        </source>
    </evidence>
    <sequence length="302" mass="33721" checksum="8FCDE45C92DD31C9" modified="2024-05-29" version="1">MFPQEGLVGDEDERFKRGNGMTSEESGKARKMMDQEGRIGENASSVQLQFLGEDVEKNSQMGEAGKNKKKRPRTTKTSEEVESQRMTHIAVERNRRKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRLYGDAPPPPRPIGDSANPMQQTQVPPPPPPFFNPMSASASDDPLKLVSDYENGLIREETAESKSCLADVEVRVLGFDAMIKILCRRSPGQLIKTIAALEDLELNILHTNITTIEQTVLYSFNVKVASESSFTAEDIANAVQQIFSFIHADSANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVQ0</accession>
    <name>A0AAF0XVQ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0832815</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVQ0"/>
    <dbReference type="KEGG" id="dcr:108198524"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF12">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH13306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="167" mass="18726" checksum="20BE3F0051B764BB" modified="2024-05-29" version="1">MNELLGLLKVKVRKGINLAIRDAVSSDPYAVVSMGRQKYKTQVIYNTCNPVWNDEITLSIKDLDLPVKLTVYDKDKFSGDDKMGEAIIVIKDYIDIIKMGLENLPDGTKVHRVQPGRNNCLAEESEIVWNKGKVTQEMILRLKNVERGEVVIGLEWVDVPGGKGLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYF0</accession>
    <name>A0AAF0XYF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYF0"/>
    <dbReference type="KEGG" id="dcr:108202614"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753:SF14">
        <property type="entry name" value="MONOACYLGLYCEROL_DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="167"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="701" mass="77977" checksum="72C566317309662D" modified="2024-05-29" version="1">MVSVLKNLWVSPCFALSSEKKPRFRVRASSVSGGKSAVVNGVGVNEEKSKVLGVEGNGRVVLNGMEDKSGNDVARKKLEPLWDDGYGKQTVKDYLELAEEFIKPDGGPPRWFCPLECGEPMEDAPVLLFLPGMDGLGMGLILHHEALGEAFEVRCLHIPVHDRTPFEDLVKFVEYSLRSEYAASPNKPIYLVGDSFGGSLALAVASRNPTIDLVLILANPATSFGRSQLQPIFPLLEALPEELQVAIPYLLSGVMGDPMKMAMVNIDTSLPRTVVTGKMSANLTALLPLLSSLSDIIPKDTLLWKLKLLKSAAAYANSRLHAVTAEVLILASGKDNMLPSKDEAQRLSTSLRNCKICYFKDNGHTILLEDDVNLMTIIKGNCKYRRSRKHDFIKDFIPPSMSEYKKAVEKYGSYRYYVSPVMLSTMPDGKIVRGLAGVPNEGPVLLVGYHMLLGTELVPLALEFLREKKVLIRGLTHPTLFSQQAASSSNEFSFFDLLKVFGALPVTPSNLFKLFSTKSHGLLYPGGAREALHRKGEEYKLFWPDQPEFVRMAARFGTTIVPFGVVGEDDIAEIVLDYNDYMRIPVVNDLIKKSNEEGIRLRTEMSGEIANQDLYFPGILPKIPGRFYFLFGKPIQTKGKENVLKNRDSANELYLQIKSEVEQNIAYLIKKRKEDPYRGILDRTVYRALDASIDEAPSFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1W9</accession>
    <name>A0AAF0Y1W9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0936192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="5"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y1W9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000009">
        <property type="entry name" value="mannosyl-oligosaccharide glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.110:FF:000002">
        <property type="entry name" value="Mannosyl-oligosaccharide glucosidase GCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF20">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE GCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="8">
        <location>
            <begin position="112"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="7">
        <location>
            <begin position="518"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH16634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="813" mass="92893" checksum="03AD728B48F07BE9" modified="2024-05-29" version="1">MTSTTGRRSARSRIKSSGTEDNDHLNSGNLKSIPKLRKHNARNNTSSILNLNLNLKVLLGFGVFTFLIALFLIYRLVNFTGDVDDKIRRLVTPFPSPKLADLPMFQGEHKESLYWGTYRPNVYVGIRARVQQSLIGGMMWIGVKDGRYFVRHVCQDSDELKKYGWTSHNGRDYGHQVIIDQEMTLETSFLKYRGKNSGYGGDWVVRSDVRTEIINDEKQRIGHLFFYLADEGNNALSLGRGVVDVHESSCLASGSREDVGNWQLHLKSANDLEVHYSGFKTPHIHNLSDLLQETLGDQVRRFGRLQLPDTSDDSANILVFQITARIPFSFDIAFLSGSGFDSSNVEERVSSLTGTQLKDKLSNKENELDDKFINCFNTSGKLDSNTVTVGKAAIGNLLGGIGYFYGESKISLPTNINAKSGNNFISYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWRWDIYICLDIVGHWLDLMNVDGWIPREQILGAEALSKVPAEFVLQHPTNGNPPTLFLVLRGKETNTYFWHGRDSLIIHELNPKTLSSGFDDYPRASHPSEEERHLDLRCWMFLAADCMHSISELFAEKIELGKKYSLTSKLLSDFELLNKMHFDNSHGTYFDFGNHSEKVRLVWQLVEDSNGFPSRKLVREVSKEPVLGLVPHIGYISLFPMIWRIIPSKSWVLGKQLDIISNKSILWTEFGLRSLGKTSSMYMKRNTEHDPPYWRGPIWMNMNYLILSSLHHYSKEDGPYRERAENIYNDLRSNLIRTVVKNYQQTGYFWEQYDQKTGKGKGARVFTGWTSLVLLIMAEGFSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIG6</accession>
    <name>A0AAF1AIG6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIG6"/>
    <dbReference type="KEGG" id="dcr:108212962"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043459">
        <property type="entry name" value="NFD6/NOXY2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156">
        <property type="entry name" value="OS02G0230000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156:SF39">
        <property type="entry name" value="PROTEIN NONRESPONDING TO OXYLIPINS 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="92" mass="9584" checksum="A7B7DDCA2B78782C" modified="2024-05-29" version="1">MASRFRSISRPISTLFKQSTSKPSSSIPFASSHIPRAPTSISRPLAQMGALQSLLPLHSAVASARLTSCLGIDSKGSRSLSQGMLCSANPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AM02</accession>
    <name>A0AAF1AM02_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0206843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AM02"/>
    <dbReference type="KEGG" id="dcr:108208471"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000048">
        <property type="entry name" value="High affinity nitrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000053">
        <property type="entry name" value="Nitrate transporter 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="64"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="490"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG87613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="530" mass="57471" checksum="C63DDD9DE66E2EB1" modified="2024-05-29" version="1">MGDIEGSPGSSMHGVTGREPVLAFSVASPIVPTDPTAKFDLPVDSEHKAKVFKLFSFANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKGDVGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFVIMLTAPTVFCMSFVADASGYIAVRFMIGFSLATFVTCQYWMSTMFNGKIIGTVNGTAAGWGNMGGGATQLIMPLLYDLIQRFGATPFTAWRIAFFIPGWLHVIMGILVLTLGQDLPDGNLGALQKKGDVSKDKFSKVFWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFDLKLQTAGIIAATFGMANLLARPFGGYASDVAARRFGMRGRLWTLWILQTLGGVFCILLGRSNSLPIAVTMMIIFSVGAQAACGATFGIIPFISRRSLGIISGMTGAGGNFGSGLTQLLFFTSSQISTAMGLTYMGIMIVACTMPVTLVHFPQWGSMFLPPSRDVVKGSEEHYYVSEWTEDEKQQGMHQGSVKFAENSRSERGRRVASAPTPPYATPNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMP3</accession>
    <name>A0AAF1AMP3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0207628</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMP3"/>
    <dbReference type="KEGG" id="dcr:108208544"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042124129" description="Subtilisin-like protease" evidence="9">
        <location>
            <begin position="27"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="12">
        <location>
            <begin position="43"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="148"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="375"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="13">
        <location>
            <begin position="656"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG88393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="755" mass="80463" checksum="18EE5E283A7BF526" modified="2024-05-29" version="1">MLPDMAILSLISLVIIFTFSAELARGNDFEPLNYVSSKNELKTYIIHVKKPEAHILAQADDLSNYHKSFMSARATAALNQEQDFLYSYKHVINGFAARLSEEDVEAMKEMEGFVSAHLEKVLHQQTTHTPRFLGLHQDTGLWRESNFGKGVIIGVLDGGILPTHPSFTDEGMPSPPARWKGKCEFVTSKCNNKIIGARSFNNSATIVSPFDQEGHGSHVAGIAAGRFVKNAAVLGLGNGTAVGVAPKAHLAIYKVCPTTDKCFESDLLAGIDAAVNDGVDVISISIVGKFAVPFFQDAIAVGSFAAIQKGIFVSCSAGNTGPSNSTVLNDAPWILTVGASTTDRSFKVTTKLGNGEEFAGESLFQPEGFSAVLMPLIYAGSSSNSNSSFCVEGSLEGIDVKGKIVLCERGLSTRVSKGETVRKAGGAAMILMNQEIDGSTTIAESHVLPAAHVSYATGLRIKAFINSTVEPKAAIVFKGTRSGDPLAPAVASFSARGPSITTPGILKPDIVGPGVNILSAWPFSVGGDSNSLSTFNVQSGTSMSCPHLSGIAALLKSAHPNWSPAAIKSAIMTTADLNNLNESPIVTERSVPADVFAIGAGHVNPSKANNPGLVYDIHPDEYIPYLCGLGYTNDQVGVITQRPINCTNETSIPEAQLNYPSFSITLASINQTYTRTMTNVGEPNSSYYIEIIPPKGVLVTVNPTSLDFTEANQQLTYRVTFGRSTETIYTQYAQGFLKWTSTHHTVRSPISVKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AN37</accession>
    <name>A0AAF1AN37_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0205829</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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    </reference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="entry name" value="IND-like"/>
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            <end position="361"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
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    <sequence length="429" mass="47670" checksum="3BCB8A49BD2A06FC" modified="2024-05-29" version="1">MADKFQAGVCAENWWMNPYQSNFGSSVCSPIINNMDMFSWPADLDTKARASTDDQSGSANNSEGSIFFQDTQKPLNHDSVEGNLLSVNSGLDLMDNGLSSSITTADWNQALFHDSKRNECNYQHSLQDSLNPSSNYQQAVGVDHFSQIKKDWSPENFSAIDEVTSLDAYKGPTNQGFPVNSSSYGYTSSLIQTLFDTNSDQDTIFGNRSMDYPDTTNLYQEKLNGFLSSSLHKPSPALPKQAPLLDNSTWSSASSNGLFSTPLQSKFLPPTFEEKLSCSNLGVKPNNERIRDMSKVVKNNSSTEPVFKRPRMETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKLLHDQVTVLINPYKRGSPIQHEQTLGDPKRADHQELRSRGLCVVPVSSTFPVASETTADFWTPTFGKNFR</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
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        </source>
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        </source>
    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="277" mass="31037" checksum="74A62B8FBB542985" modified="2024-05-29" version="1">MYSSYSGPLISCRRAVGVCCLASSCNENGQQVRTQLDKLHFEADTARAKANRARSRLIRLSEAAEMLQRQALTCIRSGRENDARDLLVRKKKVMQALENSKRRIDLLDELSAKLNEAISLKENQLVNAVTADVNVGKEVSSHDIHIVPPKEEIIVDTVESEGLDMNFLELRNESLSQVHPDCQAEQSSTSDTKDPEGTLIMNRQSETTSGSSLREISSYENFLEHLDQQLNNIEEELDTFLRFSNIILEGEGTSINLKLQQAGEILEGVSQIRKRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVI6</accession>
    <name>A0AAF1AVI6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apyrase 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0313372</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVI6"/>
    <dbReference type="KEGG" id="dcr:108210509"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24043">
        <property type="entry name" value="ASKHA_NBD_AtAPY7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF125">
        <property type="entry name" value="APYRASE 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="721"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="721"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="324"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG94081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="84291" checksum="D3706396055610D6" modified="2024-05-29" version="1">MAFGRLEEILSAAASRLSVGRPSTVQHVSPGVSPVNDSGHGVGFSRSGRNNLLRRTSSLQDFSTDSQVDPEEGTMNIGTEGRSTQEQSLLAQRVSGGASFSKKKASTGSPCMRKKWVQVILVFLCLVLFAFLVFTLQYIYSKWSKGSPEFYVILDCGSTGTRVYVYKASANHNRYVGGLPILLSSLPEGLISTKDSQIGRAYNRIETDPGFDKLVHNVSGLTGAIKPLLQWAEKQIPINAHKSTSLFLYATAGLRRLPSSDSDWLLNNAWLIMKNSSFLCQREWIKIISGMDEAFYGWIALNYHLSVLGTMPKKETYGALDLGGSSLQVTFENNKNIHNDTSLQLNIGPVNHHLSAYSLSGYGLNDAFDKSVFYIFKRLPHITKADMASGNIVIEHPCLHSGYKEQYACSHCTSLYEDDGSPLNGEGKMGKGGRPGISVQLIGAPNWGQCNLLAHAAVNISEWSGNTPAINCKMNPCALPDNVPHPNGHFYGMSGFYVVYKFFQLTSDSTLDDVLEKGQEFCAKTWNVAKNSVAPQPFIEQYCFRAPYIVFLLREGLHITDSNVTIGSGSITWTLGVALLEAGKAYTTKLEHRTYNIYWLKISPTVLLVILSASLVLLVCALLYVGNCTSKLLHRQYLPLSRHNSTSNTSVLNIPSPFRFKRWSPMQSGEGRVKLPLSPAVTSWHKPFGSVDFNSGVELNDSSMHPSSSSVVHSYSAGSLGQMQQNDSSSIGSSWSSQRSQMHLQSRRSQSREDLSASVAELHLTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYQ5</accession>
    <name>A0AAF1AYQ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0416872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYQ5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF3">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57519" checksum="49CB31ED55095D51" modified="2024-05-29" version="1">MNPFNKKVTRYYKNQTFLSILFSFTSSPKTLNPSFINPLYNPFIAKQLHTRPRFCNLSSGSASDCSVSHSTYGFSSNHHLSFLSGMLRHQFGFRWFCKLVDDTGNDKFWEFEEMGDDVFGDIENKANGVKGVDSQVNYDHVSEIVEVIRSGGSDLGLLLDSRSSNISVKLVAQIFQVLNNDKIPALHFLQWLQVSYPELCYSSRVCSSMICNCGWLEDYKTMILMLRGFKAKRVCLDETAFEFLPVLTTSQASIKDSIRKVIDALVEVGGSCCGSGISSLIKMLCSLGSFNLAEFVINITGKNVSYYNIIIREKCRKCLVGEMHGLLEEMREFGCDPNISTYNYLLSHLCVSGRMAEACSLINLMQESGTTPDALSFDILIQYSCKTGKLGIAHQFYNQILLRGIKPRIQTHVAFVKSNFSAGNCDEALEYVHNLDNQHLQSSNMLYSLVAYLHLKKGHLIVARSILIQMMDKGLKPNFLVFIKVVKRLQKSCKGGLARDLRNRFSRFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B195</accession>
    <name>A0AAF1B195_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0520163</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B195"/>
    <dbReference type="KEGG" id="dcr:108222815"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000100">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000001">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000046">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000039">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011071">
        <property type="entry name" value="PRK14501.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF130">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="829"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="841"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="869"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH00788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="935" mass="105170" checksum="AA847FD84BD8C4FE" modified="2024-05-29" version="1">MPGNKYNTNSSTPSNRIERLLRERELRKSSKASYSNETNGGGNRSSELSDADVRSHQGDGSRNSYVERYLEGALAAHALVDGFERPDGEPFRQRLLVVANRLPVSAIRRGEESWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEAGQKALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHYIGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKEHNINMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSERFIRALEVPQVQEHIRELKERFSGRKVMLGVDRLDMIKGIPQKILAFEKFLEENAYWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGSLTAVPIHHLDRSLDFQALCALYAVTDVALVTSLRDGMNLVSYEFVACQESKKGVLVLSEFAGAAQSLGAGALLVNPWNITEVASSIGLALNMSADEREKRHQHNFDHVIQHTAQEWAETFVSELNDTVVEAQQRIREVPPVLPVIEATEHYTRSSNRLLILGFNATLTEPVDTPGRRGGDQIRAMELKLHPDLKEPLLQLCKDPKTTVVVLSGSDRNILDENFGEYNMWLAAENGMFLRATKGEWMTTMPEHLNMEWVDSVKHVFEYFTERTPRSHLELRETSLVWNYKYADVEFGRLQARDMLQHLWTGPISNASVDVVQGSRSVEVRAVGVTKGAAIDRILGEIVHSKSITTPIDYVLCIGHFLGKDEDVYTFFEPELPSESIIGLPRAKTNDALKSSGERRPPSKLQNSKSSSKSSQNRSQRHLLNPEKKSNSINNGSQANGRRQSSEKISWSVLDLKGENYFSCTVGRTRSNARYMLGSSDDVVSFLKELAEGASSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4Y4</accession>
    <name>A0AAF1B4Y4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0622763</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4Y4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="21152" checksum="0421236649EEB124" modified="2024-05-29" version="1">MVRVLFWPLGSESRIEWRQIGCGVFRVSAAAGGGSGGGYSGGFGDGSSGKGDGSSGGDGEKKWSLISWYLALLKKHPVLTKSVTSAYLNLVGDLVCQVGLLCLAVIMLVTTPGVSGAFLRLVIDQLIFAPAFVGLFLSALLTLEGRPSLVLPKLQQVLAANSLALMWNVILSYIYSSQRSDRIIGMSLHLRTSAEIKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5M5</accession>
    <name>A0AAF1B5M5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0626499</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5M5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000330">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1111">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </source>
    </evidence>
    <sequence length="974" mass="106386" checksum="C11EC431EA38251F" modified="2024-05-29" version="1">MRRNLNNNNSNKKKSESSNGFLPNSFKLISSCIKTVSSNVRSASASVAGSIAADADDLKKDQVLCACFDRLELGPSVGKTLLLLGYSNGFQVLDFEDASNVTELVSRRDDAVTFLQMQPIPAKGNGREGFMDSHPLLLVVASEETRNSGPVQYGRDVSFRHSNEPHVGAAIQSPTAVRFYSLRSHSYVHVLRFRSAVYMVRCSSKIVAVGLAAQIYCFDALTLESMFSVLTYPLPHLGGQGLGGVNIGYGPMAVGSRWLAYASNNPLLSNTGRLSPQSLSPSPGVSPSTSPSSGNLVARYAMESSKHLATGIINLGDMGYRTWSKYYHELLPDGSSSPVPSSSSWKVGRTAAHSNDIDTAGTVVIKDFVSKAVVSQFRAHTSPISALCFDPSGTLLVTASIHGNNINIFRIMPLYSRNGSGTHSYDWKSSHVHLYKLHRGITAAVIQDICFSPYSQWIAIVSSRGTGHIFVLSPFGGETGLQMQTSNLNGATLLPVLSVPWWSTSSYTLKQSYPPPPPPITLSVVSRIKNSGWLNTVSNAASSAAGKLSAPSGVVAAVFHNSIGKLQPTTLNVNALEHLMVYTPSGSVIQYELLPYMGGDQGESSLRNETNSLGQVQDEDLKVKVGPVQWWDVCRRADWPEREESVGGSICSSHNAEETLMDASDYEDDGTKLKDMVKQPDRFPWYLSNAEVQMRSGRTPMWQKSQVYVHALSPLPFEGERRGLYCNDGETEIEKLPVREIVIRRKDLLPVTDHFHRIHPEWADRRDLNSGRYLPSSSLSDEGKENSGTALAEREYRNTVKSHPSFNSILNENNGDEGASSQRKQSYIGEEVNTLISPALSTTILSALEEDYVNNYTSYSNKTSPSVGNIAQEVLSSSSVVTGDVSNTSSNRSDLSMNILEEELVHEDIQGQLDFGHYFQEGYCKSKNLDQSNELTAHITDVDERASHCNKAISDEEADSDDMLGGVFAFSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCI2</accession>
    <name>A0AAF1BCI2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0831581</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCI2"/>
    <dbReference type="KEGG" id="dcr:108197674"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048825">
        <property type="term" value="P:cotyledon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000277">
        <property type="entry name" value="protein kinase PINOID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000753">
        <property type="entry name" value="Serine/Threonine Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000020">
        <property type="entry name" value="serine/threonine-protein kinase D6PK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="90"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH12082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="449" mass="49936" checksum="23C12B8669BFFB49" modified="2024-05-29" version="1">MLEYRREYSDGEETNSNTTNKNSTSSMSSSESCPSFSRLSFDALENTSLSHSPDLLTLKPHRSSDSSVQAIRSATFRRKNSGGGLGFRDFSLIRPIGSGDIGKVYLCRLKSDESCYYAMKVVNKDILAMKKKLQRAETERKILKMLDHPFLPTLHAEFEASHFSCVVMEYCPGGDLHSLRHKQPHQRFSINSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLTDFDLSLCSDPIVAVESPDISPVSSSSPSVSARSKSSPMPFSCFSTRFFRSRKIQSENRLFVAEPVTARSCSFVGTHEYVAPEVASGGGHGSAVDWWALGIFIYEMIYGRTPFAGGTNADTLRGIVKTPLTFPTEFALSGSEMHARDLISGLLHKDPSKRIGSKRGAGEVKSHPFFKNMNFALIRTVTPPEVPGLQRRNGMVCKDKNVRKSAPFELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDI6</accession>
    <name>A0AAF1BDI6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0832566</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Required for RNA silencing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RNA polymerase II, IV and V complexes. Interacts with NRPD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 14">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDI6"/>
    <dbReference type="KEGG" id="dcr:108197529"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000419">
        <property type="term" value="C:RNA polymerase V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10508">
        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034012">
        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH13057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="114" mass="13128" checksum="66E0C242315B5E54" modified="2024-05-29" version="1">MSTMKFCRECNNILYPKEDKEQRILLYACRNCDHQEAAENNCVYRNEVHHSVGERTQVLQDVAADPTLPRTKSVRCAVCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BE09</accession>
    <name>A0AAF1BE09_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0833092</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BE09"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035835">
        <property type="term" value="P:indole alkaloid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000013">
        <property type="entry name" value="NADPH-dependent codeinone reductase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="309" mass="34300" checksum="112A068A7D8C1D42" modified="2024-05-29" version="1">MALKSVPEVALSSGNAKAMPVLGLGTGGYPHLKPEEVVKACLKQFSLLGYRMFDTAAFYQTEEALGEAIAQAISRGLIKSRDELFITSKLWLHDNYGDRVLPALQKSLRDMKLDYLDQYLIHFPVSMKAGSDLFRLNPEDTVAIDIKSVWTAMEECQSLGLTKSIGVSNFSCKKLADILAFAKIPPAINQVELNPAWQQGKLRAFCQANGIKVAAYSPLGAAGAFWGTKGVLESEIALRWAYEQGIVIVMKSFNKERLKQNLEIFGWELSDEESKKIAAIPQRRANLAEFFISETGPFKTLEELWDGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WU83</accession>
    <name>A0A161WU83_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ninja-family protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ABI-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_011039</name>
        <name evidence="9" type="ORF">DCAR_0312527</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a negative regulator of abscisic acid (ABA) response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Ninja family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WU83"/>
    <dbReference type="STRING" id="79200.A0A161WU83"/>
    <dbReference type="Gramene" id="KZN02285">
        <property type="protein sequence ID" value="KZN02285"/>
        <property type="gene ID" value="DCAR_011039"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215165"/>
    <dbReference type="OMA" id="DKKEEGW"/>
    <dbReference type="OrthoDB" id="667358at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031307">
        <property type="entry name" value="Ninja_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012463">
        <property type="entry name" value="Ninja_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032310">
        <property type="entry name" value="NLS_NINJA_AFP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413">
        <property type="entry name" value="AFP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413:SF31">
        <property type="entry name" value="NINJA-FAMILY PROTEIN AFP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07897">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16136">
        <property type="entry name" value="NLS_NINJA_AFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ethylene-responsive binding factor-associated repression" evidence="6">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Tify" evidence="7">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN02285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG93246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="307" mass="33111" checksum="C3B72415DB88AD21" modified="2016-07-06" version="1">MSSGEENTGIDMLERFRTKRDSERKLGERDEGEGEIELSLGLSLNGRFGVDPKRRNNNITRAASIAGFSVGSGGEDVRAWSVAGGPDVGLSRTCSLPMDTEEWRKRKELQSLKRLEAKRKRMEKMKNVRMVRVKAGGDEVVVGPPAPQPAVVIDKKSSVQFCSQGSIGSQGSGAGSSGVSDPDTHEIPQATNNGSPASTRSSVEPIVDNKPAETVTEKPSPVLEHSGSRNEEALKNMISNMPCVSTRDGPDGKRINGFLYRCKSGEELKIICVCHGEFLSPAEFVKHGGGGDVENPLRHIVINRAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X3T7</accession>
    <name>A0A161X3T7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0728004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2+) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant rapid alkalinization factor (RALF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X3T7"/>
    <dbReference type="Gramene" id="KZM87103">
        <property type="protein sequence ID" value="KZM87103"/>
        <property type="gene ID" value="DCAR_024237"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008801">
        <property type="entry name" value="RALF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34270">
        <property type="entry name" value="PROTEIN RALF-LIKE 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34270:SF3">
        <property type="entry name" value="PROTEIN RALF-LIKE 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05498">
        <property type="entry name" value="RALF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH08561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="74" mass="8094" checksum="0A21043BA345EC2A" modified="2016-07-06" version="1">MALSKKSVIFCASIILFIVLVSEVAEADKFISYGAMYANGRKCRNRSGCIVRDTPANPYNRGCQASQRCRGGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XDW1</accession>
    <name>A0A161XDW1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0624467</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XDW1"/>
    <dbReference type="Gramene" id="KZM90893">
        <property type="protein sequence ID" value="KZM90893"/>
        <property type="gene ID" value="DCAR_021742"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228019"/>
    <dbReference type="OMA" id="DQKVFQG"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000268">
        <property type="entry name" value="31 kDa ribonucleoprotein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF3">
        <property type="entry name" value="31 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH05055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="325" mass="35807" checksum="0C69DF865CEAC211" modified="2016-07-06" version="1">MSSTTPPLIQTSRFSIPPKCSCISTLKPSSFLSLPAKPISLLHSSSSLWLSHKPSKILRPILVAEASEYGQEEGGEEGGVSSVSTIEPEASVEEASQEAVEEVVETQEEEVVVAEAEEVVFAEEEVVVEEEGEEVVEEEESYSEPPEEAKLFVGNLPYDYDSARLAELFNKAGVVEIAEVIYNRQTDQSRGFGFVTMSTVEEAEKGVEMFSRYDLGGRLLTVNKAAPRGSPPQRVFEPSFRIYVGNLPWEVDNGRLEQVFSEHGKVIDARVVYDRETGRSRGFGFVTMASETEVNDAIAALDGQSLGGRAIRVNVAEERPRRNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XYY0</accession>
    <name>A0A161XYY0_DAUCS</name>
    <protein>
        <submittedName>
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    </protein>
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        <name evidence="2" type="ORF">DCAR_010711</name>
        <name evidence="3" type="ORF">DCAR_0312174</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
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        </location>
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        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN01957.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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        </source>
    </evidence>
    <sequence length="1151" mass="130115" checksum="668EF869C75EDE07" modified="2016-07-06" version="1">MASDFKETGLKKLEYLSLVSKVCTELESHLGFGDKVLAEFIIELGKNCVSVDDFYVKLKENGAEMPDYFVRTLLTIIHAILPPELKLGDDKEEVGEGSSCKSSFPGLNIGDSKDRVRELEREIAFEAEERRRGKGPRECMYRDGGRGKSKSRHGGERERCRDRDVGDERGDYRKASGNERDGGNEGDGELKCVEPELYRIYRGRVSRVMDSGCFVRLDDFRNKEGLVHVSQIASRRIPNAKDVVKRDQEVYVKVISISGHKLSLSMRDVDQNTGDDLLPLKKSSGDVDVVRANPSASHKGSATRTSLSGIRITEEDDAGPSRRPLKRMSSPERWEAKQLIASGVLSVNEYPMFDDETDGLLYQEEGAEEELEIELNEDEPAFLNGQTRFSMDLSPVKIFKNPEGSLSRAAALQSALIKERREVREQQQRAMLDSIPKDLNRPWEDPMPETGERHLAQELRGVGLSAYSVPEWKKDTDSKILTYGQRSKLSIQEQRQSLPIYKLKKELIQAVHDNQVLVVIGETGSGKTTQVTQYLAEAGYTTHGKIGCTQPRRVAATSVAKRVAEEFGCRIGEEVGYAIRFEDTTGPDTVIKYMTDGMLMREILIDENLSQYSVIMLDEAHERTIYTDVLFGLLKKLVQKRRDLRLIVTSATLDAEKFSGYFFNCNIFTIPGRTFPVEILYTKQPESDYLDAALITVLQIHLTEPAGDILLFLTGQEEIDYACQCLYERMKGLGKNVAQLIPLPVYSALPSEMQSRIFEPAPPGKRKVVIATNIAEASLTIDGIYYVIDPGFAKQNVYNPKQGLDSLVITPISQASAKQRAGRAGRTGPGKCYRLYTESAFHNEMPPTTIPEIQRINLGFTTLSMKAMGINDLLSFDFMDPPSPQALISAMEQLYSLGALDEEGLLTKLGRKMAEFPLDPPLSKMLLASVDLGCSDEILTIIAMITTGNIFYRPREKQAQADQKRAKFFQPEGDHLTLLAVYEAWKAKNFSGPWCYENFVQLRSLRRAQDVRKQLVAIMDKYKLDVVSAGNNFTKIRKAITAGFFFHAARKDPQEGYRTLVENQPVYIHPSSALFQRQPDWVIYHELVMTTKEYMREVIVVDPKWLVELAPRFFKVADPTKMSKRKRQERIEPLYDRYHEPNSWRLSKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZL34</accession>
    <name>A0A161ZL34_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_025003</name>
        <name evidence="2" type="ORF">DCAR_0728705</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZL34"/>
    <dbReference type="Gramene" id="KZM87902">
        <property type="protein sequence ID" value="KZM87902"/>
        <property type="gene ID" value="DCAR_025003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM87902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="95" mass="10301" checksum="509C31710E9D2429" modified="2016-07-06" version="1">MESSGLVLEKFVKFVTEHDVLSDDMKLFHHFQGEDKSVVVAAVRADSGRGGDGFGWRRNSGGCGRSDDGFGWRRSGDSGCGSDGFGWRKNHFGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZX76</accession>
    <name>A0A161ZX76_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_020945</name>
        <name evidence="5" type="ORF">DCAR_0625389</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZX76"/>
    <dbReference type="STRING" id="79200.A0A161ZX76"/>
    <dbReference type="Gramene" id="KZM91690">
        <property type="protein sequence ID" value="KZM91690"/>
        <property type="gene ID" value="DCAR_020945"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225549"/>
    <dbReference type="OMA" id="EQRWILY"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000775">
        <property type="entry name" value="BnaA09g28200D protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045182">
        <property type="entry name" value="JINGUBANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844">
        <property type="entry name" value="F-BOX AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22844:SF336">
        <property type="entry name" value="PROTEIN JINGUBANG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="304"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="390"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM91690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="458" mass="50288" checksum="0B8A6C82CA9BAD47" modified="2016-07-06" version="1">MREDKGRLTSVRLGGVSLSPLKLGNMMHLDANISLATTKIDDDFSDTKGGFGAYEQARLSGEGSPIMMSPWNHNSPFTPWSNFAAVQNPDVPQTKPPQIALTGSLVREEGHIYSLAATRDLLYTGSDSKNIRVWKSLKEFSAFKCNSGLVKAIIICGDKIFTGHQDGKVRVWKVQARNPNVHRLSGTLPTLMDIFRCSIKPKNYVEVKRNKTSVWIKHGDAISSLSMNQEKGLLYSASWDRTFKVWKVDNSKCLESVKAHEDAVNSIVTGANDTVFTGSADGSVKVWRREAYKKGTKHIFVQTLLSQECAVTSLAFNETTSVLYCGSSDGVLNFWHCEKHLSHGGVLKGHRLAVLCLAAAGNLVLSGSADKTICVWKKLEGSAHTRLSVLTGHTGPVKCLAVEKDEAISETDDQQWVVYSGSLDKSVKVWSVSEQSPELSEMQQHGVSRWGAIPSAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A1Z4</accession>
    <name>A0A162A1Z4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_018174</name>
        <name evidence="8" type="ORF">DCAR_0520802</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A1Z4"/>
    <dbReference type="STRING" id="79200.A0A162A1Z4"/>
    <dbReference type="Gramene" id="KZM94932">
        <property type="protein sequence ID" value="KZM94932"/>
        <property type="gene ID" value="DCAR_018174"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220421"/>
    <dbReference type="OMA" id="ALIVICH"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000419">
        <property type="term" value="C:RNA polymerase V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028363">
        <property type="entry name" value="RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500154">
        <property type="entry name" value="RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM94932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH01418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="145" mass="16613" checksum="B8A66A3A651A02D1" modified="2016-07-06" version="1">MADEDFEMDGGYEDEPLEPELDEGAEEDVDADANKEDDVADPLLADGEEKQEEEPAERPRKTSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQVGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ADH0</accession>
    <name>A0A162ADH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_013046</name>
        <name evidence="11" type="ORF">DCAR_0418051</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of a prohibitin multimeric complex in mitochondrial membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG98707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A162ADH0"/>
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        <property type="protein sequence ID" value="KZM99592"/>
        <property type="gene ID" value="DCAR_013046"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219433"/>
    <dbReference type="OMA" id="DTHMPPP"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
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        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="37"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM99592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG98707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="288" mass="31434" checksum="58394C0EFF61FE75" modified="2016-07-06" version="1">MNFRNVKVPNVPGGGAASALLKVGAVAALGLYAVGNSLYNVDGGHRAIVFNRIVGVKDKVYPEGTHLMLPWFERPIIYDVRARPHLVESTSGSRDLQMVKIGLRVLTRPMASQLPTIYRTLGENYNERVLPSIIHETLKAVVAQYNASQLITQREAVSREIRKILTERAANFNLALDDVSITTLTFGREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAISSNPAFITLRKIEAAREIANVMASGNNKTYLNSGDLLLNLHGMDVTPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AHG8</accession>
    <name>A0A162AHG8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_009887</name>
        <name evidence="2" type="ORF">DCAR_0208810</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AHG8"/>
    <dbReference type="Gramene" id="KZN01133">
        <property type="protein sequence ID" value="KZN01133"/>
        <property type="gene ID" value="DCAR_009887"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN01133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG89572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="79" mass="8909" checksum="04252B81058D33BA" modified="2016-07-06" version="1">MYFGDAILASTNTCAYSYWPTNFYMQSICVDKFIPKLGVFKSRVQCPLEEVNELMKVDQVLEFMAFVGPGISKIAQADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ALN6</accession>
    <name>A0A162ALN6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0103501</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Frigida family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_003302"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Frigida"/>
    </dbReference>
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        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INACTIVE FRIGIDA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0287">Flowering</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG84318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="257" mass="29951" checksum="0B822B31B21ED02B" modified="2016-07-06" version="1">MGEMMNVMKKKEEVKNREDELDEMLVEIDLSKRNVIEMEKQVEFKEKIIQIHETNMNKAWAELEMKSKEASSVEYSIVKPEPWSDDGSYADIRFSVTMDGNSLLLYLINHKGDLDSMSDEVYEALGKSKEPGKLVLDALQSFYSKKEAEEFEEDVVCRSTSLLLEQLRRISPHIQSYHKRAALKLASQWKEKMKSSKEFIVFLQLLASYKLESSFHPEEFFSLFEVINQPTEISELFQLLNYMGKVNGQVNQQHEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AQD8</accession>
    <name>A0A162AQD8_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0205768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN04343">
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        <property type="gene ID" value="DCAR_005180"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="108" mass="11830" checksum="1B2568D585F75D68" modified="2016-07-06" version="1">MDADRMRRSAPDEEDDEFVTPLERFDSSGKKTAGQAGRKYGCSSLSAKSRVGTASIGKNLRSKTLQNNSDARDVSALSKPDRPRVWSSSSRNPGEFAIMERGNSLMCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AXR6</accession>
    <name>A0A162AXR6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0209259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AXR6"/>
    <dbReference type="Gramene" id="KZN07353">
        <property type="protein sequence ID" value="KZN07353"/>
        <property type="gene ID" value="DCAR_008190"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206746"/>
    <dbReference type="OMA" id="MKEMDYA"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010283">
        <property type="term" value="F:pinoresinol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009807">
        <property type="term" value="P:lignan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF4">
        <property type="entry name" value="PINORESINOL REDUCTASE 1-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
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    </dbReference>
    <dbReference type="Pfam" id="PF05368">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="4">
        <location>
            <begin position="2"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG90018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="35243" checksum="222730448CC76CBA" modified="2016-07-06" version="1">MEKSKVLVVGGTGYIGKRIVQASLAQGHITYVLQRHEIGLDIDKLQLLLAFKKQGARLVEASFSDHQSLVNAVKQVDVVICTMSGVHFRSHNITMQLKLVDAIKEAGNIKRFLPSEFGMDPARMANALEPGRVTFDEKMVVRKAIEDANIPFTYVSANCFAGYFVPNLSQLGTLLPPKNKVCLYGDGSAKVVYMDEDDIATYTIKSIDDPRTLNKTLYIRPKENILTQMQLIEKWERLSGKKLEKLNISAEDFLASMEGMDYASKVGVGHFYHIFYEGCLTNFEIREDGSVEASQLYPEVDYTRMDSYLNQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B694</accession>
    <name>A0A162B694_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_003199</name>
        <name evidence="3" type="ORF">DCAR_0934573</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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        <location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="312" mass="34725" checksum="2A41F950A16D8998" modified="2016-07-06" version="1">MEESGVIGNGRGRNKRFWTAEEDTVLVSALLELASDPHWKCDNGFRNGYMVRLEEIIGKALPTCGLKATPHIDSRLKTLVSKFRAIAQMLSTSGFMWDDDRKMISVDRSVYDEYCKTHTTCKNLYGVAFPHFHELMTIYGKDYATGKPAEGFVDAVNSMEKAAPIQVTLDSSDEEIDVSGNVTQLDESEAPPSKKAKVEKTKKIGKKSESVAASEISSLQSFMKDMNVHLSTMANVMSRADEREQELAEKSEKVIEELLSFNLEGVTTTQVFEVADILTSQPNKLMIFNKCPSSLKAAFVKNLIGENSRRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG84286.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="23209" checksum="85DE695E547F303A" modified="2016-07-06" version="1">MANSSSNVSNSSFQEESRDVYEAALLLYNMKHITLDPQAAHQLEQEKARQKYILQCSRPNYQDHLPPVQGLVGNYCSKPFQKQLTKSDLKKDQQRLLLNKSHVKQFLYPLLSSGEVKDVENREIGVHVYDAEGKVYEMKFKLWAEKAYVLKTNEWLRFCSEHGLVETKDWITIWMFKHATDTHQLCFAIIPNYNLLPSL</sequence>
</entry>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EMBL" id="WOH00093.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="66" mass="7766" checksum="D8442E6CBD59C9E7" modified="2016-07-06" version="1">MLPLFLTVAFSAVPLTLYLPPIRNLCLFLVSVDDLFRQTGVYSLHRLRFGHRLRIAFSRFLSRFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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        <name evidence="7" type="ORF">DCAR_018232</name>
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            <taxon>eudicotyledons</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH01483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YVD2"/>
    <dbReference type="STRING" id="79200.A0A164YVD2"/>
    <dbReference type="Gramene" id="KZM94974">
        <property type="protein sequence ID" value="KZM94974"/>
        <property type="gene ID" value="DCAR_018216"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM94990">
        <property type="protein sequence ID" value="KZM94990"/>
        <property type="gene ID" value="DCAR_018232"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223631"/>
    <dbReference type="OMA" id="RECVETY"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.170:FF:000003">
        <property type="entry name" value="Exocyst subunit Exo70 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF96">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT EXO70B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="264"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM94974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM94990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH01483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="624" mass="71333" checksum="9A13CBDEBF0B7FA0" modified="2016-07-06" version="1">MAENGEEKLIAVARHIAKTLGHTDNTADDILQIFSNFDGRLREKLSEKLIDDDDVLKNSSLSSLDQNLKSLDRRISRYLSADRLIWSDSADSAAFLDAVDDLIAVIRDWTPMAAEKSVASSLDRAEDLLQQSMFRLEDEFKSLMERGGESFDVTRYSNGESTGFDSDEEIDDDENDDDEEIPVAHRISDFNIVIDALPSGTINDLHEIAKRMVIAGYGKECSYAYSSYRREFLDESVSRLGLQKLSTDEVQKMQWADLEEEIERWIKAANVAVRILFPSERRLCDRVFYGFSNASDLSYMEVCRGGMTQLLQFADAVAIGSRAPERLYKVLDVFEALRDLIPHIQMVFADQYCLFMCNEAISIWKKLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYIRVACQSRQTLEQVFEDGVSSPVDRPSSSSSLSVQMSWMMELLESNLEAKSKIYRDTALASVFMMNNGRYIVQKVKDSELGSLLGDDWTRKHTAKVKQYHVNYQRSSWSKVLQVLKLDNNSSSPTVELRAMKEKIKLFNNQFDEICKIQSNWVIFDEQLKQELRISVFSNLSMAYRSFLGRVQSVPDTGRQMERHIKYTVEDLESKINELFRGNGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A6Y7</accession>
    <name>A0A165A6Y7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_015624</name>
        <name evidence="10" type="ORF">DCAR_0415104</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A6Y7"/>
    <dbReference type="STRING" id="79200.A0A165A6Y7"/>
    <dbReference type="Gramene" id="KZM97014">
        <property type="protein sequence ID" value="KZM97014"/>
        <property type="gene ID" value="DCAR_015624"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215972"/>
    <dbReference type="OMA" id="GIFLWCI"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048354">
        <property type="term" value="P:mucilage biosynthetic process involved in seed coat development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009825">
        <property type="term" value="P:multidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000013">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033254">
        <property type="entry name" value="Plant_FLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382:SF0">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAS1" evidence="8">
        <location>
            <begin position="31"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="8">
        <location>
            <begin position="191"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM97014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="411" mass="44248" checksum="5BE33A3683494676" modified="2016-07-06" version="1">MATTPPSHLSNFTPITLLFLLLLSPLPILSFTNITTLLSSYPDLSDFTNLLLSTAIPLDLTRRTSLTILAVPNAFLRSSTAAPRPDIADILRYHVLLQYLSLSDLKKFSKSGKIITTLFQTTGRAPNNFGSINVTHDPVTDVTSFVSPESNATLLSVIKSVPYNISIFSVNNLLVPVRADLLASESGPRIDLNITKAITDGHNFNVAVAMLQASGVIDEFEADENGAGITLFIPTDESFADLPPSARFQSLPADKKAVVLKFHVLHSYYPLGSLESIVNPAQPTLATEDTGAGSFTLNISRVNGSVSINSGIVQASVTQTVFDQKPVAIFGVSRVLLPREIFGNNPIIFKPSIGGHYAIAQPPQTAVSPESSQASSSPPVLQREIKSEATRLDRFDIAGFCCTLLLYILSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A7K7</accession>
    <name>A0A165A7K7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0415137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 11S seed storage protein (globulins) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A7K7"/>
    <dbReference type="Gramene" id="KZM97041">
        <property type="protein sequence ID" value="KZM97041"/>
        <property type="gene ID" value="DCAR_015597"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216871"/>
    <dbReference type="OMA" id="VTWWFNP"/>
    <dbReference type="OrthoDB" id="336321at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02243">
        <property type="entry name" value="cupin_11S_legumin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02242">
        <property type="entry name" value="cupin_11S_legumin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006044">
        <property type="entry name" value="11S_seedstore_pln"/>
    </dbReference>
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        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF62">
        <property type="entry name" value="OS01G0976200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00439">
        <property type="entry name" value="11SGLOBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="2" id="KW-0758">Storage protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG95808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="356" mass="38182" checksum="1EC31D7F0780C64F" modified="2016-07-06" version="1">MDIDLTPKVAKKVYGGDGGSYHAWCPNDLPMLKQGNIGAAKLSLNKNGFALPSYSDSAKVAYVLQGSGVAGIVLPEKEEKVLPIKTGDSIALPFGVVTWWYNKEDTELVVLFLGDTSKAHKSGSFTNFYLTGSNGIFTGFSTEFVGRAWDLEESVVKTLVGNQSGTGIVKLDAGFKITEPSEKHRNGLVLNCLEAPLDVDIKNGGRVVVLNTKNLPLVGEVGLGADLVRLDGSAMCSPGFSCDSAYQVTYIVRGSGRAQVVGIDGKRVLETTVEAGNLFIVPRFFVVSKIGNPEGLEWFSIISTPNPVFTHLAGSIGAWKSLSPQVLQASFNVDSDVEKQFRSKRTADAIFFPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165X7U2</accession>
    <name>A0A165X7U2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0416166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165X7U2"/>
    <dbReference type="Gramene" id="KZM97911">
        <property type="protein sequence ID" value="KZM97911"/>
        <property type="gene ID" value="DCAR_014727"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216750"/>
    <dbReference type="OMA" id="ETDCLSG"/>
    <dbReference type="OrthoDB" id="764087at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040376">
        <property type="entry name" value="At4g28100-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34056">
        <property type="entry name" value="GPI-ANCHORED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34056:SF1">
        <property type="entry name" value="GPI-ANCHORED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG96829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="315" mass="34821" checksum="6744CE0FF036FBA3" modified="2016-07-06" version="1">MFHHFPILFWSHLLLFSPPLSLQSPPPTIPAFIEQSDVSSACTLRFSDDLFTGINDACAATKTKLNSPQLHRSRCCPVLAAWLYTAYSDTALGRISHVPEKTSDLPMLPDESETCVKNLEKGLTNKGMKLVKPNETCDLVYCYCGITLHPLSCPESFFVSQKTGKLVGDSTVKKLEKNCLSTDPNGLIGLDGCSKCLNTLHLLNENKTSNSSKSEERNDKMHNRDCELMGLTWLLAKNRSAYIHTVSSVLRAIMMSKDGSSPQSCSLNSDGMPLAVNSFEINSQPSIPRLMSPVYLLVTTLSLSYMASVYNLEIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YGC5</accession>
    <name>A0A165YGC5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_013582</name>
        <name evidence="2" type="ORF">DCAR_0417438</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YGC5"/>
    <dbReference type="STRING" id="79200.A0A165YGC5"/>
    <dbReference type="Gramene" id="KZM99056">
        <property type="protein sequence ID" value="KZM99056"/>
        <property type="gene ID" value="DCAR_013582"/>
    </dbReference>
    <dbReference type="OMA" id="FNKMGWP"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896:SF2">
        <property type="entry name" value="CBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM99056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG98097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="96" mass="11116" checksum="20CE873DD1419FA8" modified="2016-07-06" version="1">MTAYFIKTKWPEKAPKSEEERKDFIASVHKRKTMLFMVLIEKKLFPLRPGVAKLIDQTFAKGVKVAVCTTSNEKALLRSLKLYSSRKLQRQNTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z432</accession>
    <name>A0A165Z432_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0418044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z432"/>
    <dbReference type="Gramene" id="KZM99585">
        <property type="protein sequence ID" value="KZM99585"/>
        <property type="gene ID" value="DCAR_013053"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219524"/>
    <dbReference type="OMA" id="CIPVPQT"/>
    <dbReference type="OrthoDB" id="1911783at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF28">
        <property type="entry name" value="OS04G0488500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="335" mass="37837" checksum="93CCBA64B7B4BC1F" modified="2016-07-06" version="1">MFKLLIPKRPGHSFVTFSSIHSVCLSLEESVRAAVESRKYRQIPDLFTASKECCPASNPFSFLSTFSLARRTQVIDEMLQDFIWIRPRYHPYATYSCLLTYVLQSPDPLPLGLAIVQRVLRSGCVPVPQTHLLLSSAWMERRQQCESVSKMLLEMESIGYGPDCGTCNYLISSLCKVDQSKEAVQVLRGMCKSGCVPDLDTYGIVIGAMCRIRRITVVAELMREMVKFGLTPRQEIVVKVIKTMKARKDVWRAVEVIELLQTEGILVGFESYESVLEGCLECGEYVLAGKVVMGMTNRGFIPYIRARQKVVEGLANAGDWKFAYAVRQRFTELKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZA95</accession>
    <name>A0A165ZA95_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glycosyltransferases</fullName>
            <ecNumber evidence="15">2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0418275</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the synthesis of glucuronoxylan hemicellulose in secondary cell walls.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Golgi apparatus membrane</location>
            <topology evidence="1 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZA95"/>
    <dbReference type="Gramene" id="KZM99799">
        <property type="protein sequence ID" value="KZM99799"/>
        <property type="gene ID" value="DCAR_012839"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215581"/>
    <dbReference type="OMA" id="AFQAYFL"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00218">
        <property type="entry name" value="GlcAT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000064">
        <property type="entry name" value="Glycosyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF65">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="12">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="14">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG98929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="387" mass="44535" checksum="A34075240D8D55AE" modified="2016-07-06" version="1">MASIRRSLSPYHDRSHTNGGSPFSVNSPSHKNANRSSSYSLSRFLAALSLQRHSRKAQPFWRKSLFRCLILFMLGFLLGLAPFNEVDVVSNHDFTFEIIKPPPVNVIENDVVMNHPVQLGERKLEKSVELVYRKELIVVTPTYNRALQAYYLGRLGNLLRLVPPPLIWIVVEMNEASMETAEILRNTGVMYRHLVCTKNSTSVKDRGVHQRNTALEHIERHKLDGIVYFADDDNVYSLELFDNIREIRRFGTWPVAMLAQSKNKAVLEGPVCNGSAVIGWNTNEKSKILRRFHVDMSGFAFNSTILWDPKRWKRPTTEPIRQLDTVKEGFQETTFIEQVVEDESQMEGIPQDCSRIMNWHLHIEARDLVYPDGWLLPKNLDVVLPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AYW6</accession>
    <name>A0A166AYW6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AYW6"/>
    <dbReference type="Gramene" id="KZN02126">
        <property type="protein sequence ID" value="KZN02126"/>
        <property type="gene ID" value="DCAR_010880"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215383"/>
    <dbReference type="OMA" id="DCMVTED"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000925">
        <property type="entry name" value="F-box protein At5g67140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF95">
        <property type="entry name" value="F-BOX PROTEIN YLR352W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG93074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="230" mass="25821" checksum="32A38DEC0D51BBEE" modified="2016-07-06" version="1">MESRRGEAEIDRLPIDLLSHILVFITCFKDLAQASRVCKKWREGVKRSLGHREKLSFVGWKMDDDSATRILFHAYSLKELDISKSRWGCRITDKGLHQISLAKCLGNLSSISLWGMTEISDNGIRQLVSGATSLQHLNIGGTFITDESLFAIANSCPHLKSINLWCCRHVTEHGLLVLVNKCQKLESINVWGMRVPMECFVCLVNISPALHLQPEGLLPLAERASLLPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B3W8</accession>
    <name>A0A166B3W8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0312573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B3W8"/>
    <dbReference type="Gramene" id="KZN02328">
        <property type="protein sequence ID" value="KZN02328"/>
        <property type="gene ID" value="DCAR_011082"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210629"/>
    <dbReference type="OMA" id="MDIYRST"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070417">
        <property type="term" value="P:cellular response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14481">
        <property type="entry name" value="SPX_AtSPX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031142">
        <property type="entry name" value="SPX_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978:SF7">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG93292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="306" mass="34531" checksum="B5523F4DDEF2ECF7" modified="2016-07-06" version="1">MKFGKEFSIHLEETLPEWRDKYLRYKPLKKLLKNFPDSSSVSNLPPPAAGADASPPLPQHLQEWFVKILSEELEKFNDFYVDKEEEFVIRFQELKERIERLKEKSCKDGVFTSESEFSDEMMEIRKDFVSIHGEMVLLKNYSSLNFAGLLKILKKYDKRTGGLLRLPFTQLALDQPFFTTEPLTRLVHDCEANLEILFPLEAEIVEPTSVVRGTNSTASNMATISGEATSLGEDTGEVYRMTVAAMRTIQGLKRESSTYNPLSFASIFGCRDNDSSGAVTGGNSDSDSIDSLQNKEPKQDDAPSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BW13</accession>
    <name>A0A166BW13_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0313217</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BW13"/>
    <dbReference type="Gramene" id="KZN02952">
        <property type="protein sequence ID" value="KZN02952"/>
        <property type="gene ID" value="DCAR_011708"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211018"/>
    <dbReference type="OMA" id="WRSAKEC"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG93929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="388" mass="45023" checksum="9ACB05106FB99181" modified="2016-07-06" version="1">MGKGGRMSVQPVANTEAEKNESIVRRVPHQKPPFTIADIKKAIPPHCFNRSLIRSSSYLVFDIAVCFFLYYIATNYIPLLPAFLSYVAWTAYVYVQGCFMFAIWVVAHECGHHGFSDYHLLNDALGFILHSLLLVPYFSWKISHRRHHANTNSLDRDENHVPRFKNTIRSYYHHFNNPLGRVFIITFTLTLGWPLYLIVNIAGRSYDRFASHFDPYSPIYSERERLQIMLSDAGFVAACYGLYRIALVKGFAWVFLVYGAPLHVVNGFLVMITLLHHTHLSLPHYDSSEWDWLRGALATVDRDYGILNKVFHHIADTHVLHHLISSIPHYHAQEATEAIKPVLGEYYHYDGTPFYKAMWREAKECLYVEEEDEGDNKTKGVYWYKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D583</accession>
    <name>A0A166D583_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0206271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D583"/>
    <dbReference type="Gramene" id="KZN04739">
        <property type="protein sequence ID" value="KZN04739"/>
        <property type="gene ID" value="DCAR_005576"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207339"/>
    <dbReference type="OMA" id="PIIEMNG"/>
    <dbReference type="OrthoDB" id="764273at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055301">
        <property type="entry name" value="Lea14-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31852">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG87051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="222" mass="24337" checksum="DB7354C704D7C32B" modified="2016-07-06" version="1">MPEEEQDYSRSAESHRIVIPNEVATPRSSSRKQSLQRRCWLCCAFTSTLIFITGIVLLILFLTVFKVKKPKVSFTSLSIAGLQGVNPLNLGDSANLTVVVDVSVKNPNVASYKFKNGTTEMYYKKVQVADAHIPAGNAKARRTMHMNVTADFMLSNIASVDGLVSDLMNGSLPLQTSTSLKGKVNIINIVKKKVAVKVNCSLTFLIANQTLQDLKCKSDVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D933</accession>
    <name>A0A166D933_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_005785</name>
        <name evidence="12" type="ORF">DCAR_0206511</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D933"/>
    <dbReference type="Gramene" id="KZN04948">
        <property type="protein sequence ID" value="KZN04948"/>
        <property type="gene ID" value="DCAR_005785"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210219"/>
    <dbReference type="OMA" id="EMEINRM"/>
    <dbReference type="OrthoDB" id="1357022at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="170"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="423"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="673"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN04948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG87288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114734" checksum="5176DB856C9C054D" modified="2016-07-06" version="1">MSDAIIGDLASGLIRKLVSLATEEIILAWNLLDDLVRLRQRMESIDALLLDAATKKLTMSAVQTWFNQIEAVAHVADVFMDELAYQVTRHKVQHHRVWDFLIPSKKSSLLYRFKVAHKMKSINTSFDNIFTSAGELGLQPVAHLLATMQTRLIRKTPHSEDKSLLVGRDDDISYLVQMVCKNHEVELPVTAVYGMGGQGKTTVARMVYNRDAVINMFPKRMWITVSEDFDFMKILNQMVVSLTSTASVLENTEGLIKELQKNLKGEKFLLVLDDVWNEKPEEWDNLRNSLLEIGGARGSNILITTRSQEVAYAMRCSVSYQVKILSEEDSYELFKRIAFSHGGIVETEAFEELGRRLVKRCGGLPLAIKTLGGLLHSKESEQEWLEIQNSEIWKSKGVLASLRLSYDNLPYSSLKRCFAYCSIIPKDTDICKDELAQIWKALGFLLPARGSTALMEDIGYEYFNILLCNSLLQDVEKDAVGNITSCKMHDLVHDLALDLSKYHSVTVKTGHELNIVSHISQPIYLRLDERISNRNPAILKRNLERVQALYTGARFLGDMLPYLKHLTVLVLNANEITSELPSSLSKMKYLKYLDISCFRGILPSYITDLYNLETLRVWALQVLPKEFCNLINLRHLYIVNPHERCMFIGIENLTCLQTLPHFVVSPDHSCLVKNLGGLNNLRGKLDLYGLDNVEDMVEATQAKLCLKPNLQSLVLEWDTNTSLRVDEEYNDEEVMRGLKPHTNLKELKIEYFKGKQLASWIAMMTNLVKITLRYCSRCEGLPTLGHLPKLREMEINRMKNVKFIGDNSSGGRGSGGTEFTASWGTKTNTTMYPSLTKLRFFDLPELEEWLESVMSTCDEDRSTMLAFPKLEVLEINGCPKLTRIPGSCFPSLKLLFIVDSDSSSMILETISRNVSSLAYLRLENISNGGGGSSSSSSSSKMDSIIDQLLENNFKSLETLKLYDCKGLTSLKLGPAIKELEVSYCHDLTSINLVEDSSVLNYVTIWGCPSLSNWISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DA62</accession>
    <name>A0A166DA62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_005827</name>
        <name evidence="21" type="ORF">DCAR_0206555</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DA62"/>
    <dbReference type="Gramene" id="KZN04990">
        <property type="protein sequence ID" value="KZN04990"/>
        <property type="gene ID" value="DCAR_005827"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210211"/>
    <dbReference type="OMA" id="ESCWTSF"/>
    <dbReference type="OrthoDB" id="780646at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000287">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000390">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
        <property type="entry name" value="SPARK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF62">
        <property type="entry name" value="OS05G0423500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19160">
        <property type="entry name" value="SPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872083" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="21"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="289"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KZN04990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG87331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="618" mass="67992" checksum="A4FDEBEE23ADDA7F" modified="2016-07-06" version="1">MSRSHLFIITLLGFCSLCSSQSPTKCPLDFNVLRRLAEGSRPSDKVADQCLLILQGLRLVQSDYLRRTNKFLPPLSSAESCWKDYQGNFDQLGNSFDIRSTCGFETQWIAEGCQNITTREEYESRNSRNALNGVSQACNQSLNGAPCATCTTSLSALLPSDVKGEKVGNVSDCTAFKSIYAAAFANSYGPTDRGTAECLFALTFDRSNKDESRRKILIGVFSGIGGLIIVVGVCGFLWFRKKKIERAKRKEEITKKWTELNSSALDSISGSTTLIKFSFDEIQEATKNFSRLNIIGKGGYGNVYKGVLPDGTEVALKRFKNCSAAGDADFSHEVEVIASVRHVNLVALRGYCTATTNFEGHQRIIVCELMKNGSLHDHLFGSDEKKLSWPLRQKIALGTARGLAYLHYGAQPTIIHRDIKGSNILLDDDFEPKVADFGLAKFAPEGATHVSTRVAGTMGYVAPEYALYGQLTERSDVYSFGIVLLELLSGKKALLEIDEDQHTLVADWAWALVRTGRPLDVVEEGIPELGPPDVMEKYVLLAVLCSHPQLYARPTMDQVVKILDSDLPVPMIPERPIPITAAIDDIEKSVSTMNSGNLSTYSGYQPYIDNSAPNTPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166E5Z8</accession>
    <name>A0A166E5Z8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="4">1.4.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0207882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN06141">
        <property type="protein sequence ID" value="KZN06141"/>
        <property type="gene ID" value="DCAR_006978"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210067"/>
    <dbReference type="OMA" id="SEICWYF"/>
    <dbReference type="OrthoDB" id="434253at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF3">
        <property type="entry name" value="PYRIDOXINE_PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG88647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="22699" checksum="19F1D2F90FB4F776" modified="2016-07-06" version="1">MGTTAIAPWKQLLLNAMQSNSHLKHSSYFQLATIGINGRPANRTVVFRGFQDNTNKIQINTDSRTHKIEDLKQCPFGEVCWYFTDSWEQFRINCVVDVIDGSNPDPIKLQQREKAWFASSPRSRLQYVGPYPGLPSISEQSSEESSLDDSTGPVDAFCLLVLNPEQVDYLNLKENKRVNFTARQSNESEKLWNSERINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EJ71</accession>
    <name>A0A166EJ71_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_007473</name>
        <name evidence="4" type="ORF">DCAR_0208434</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EJ71"/>
    <dbReference type="STRING" id="79200.A0A166EJ71"/>
    <dbReference type="Gramene" id="KZN06636">
        <property type="protein sequence ID" value="KZN06636"/>
        <property type="gene ID" value="DCAR_007473"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208818"/>
    <dbReference type="OMA" id="DYPPIFH"/>
    <dbReference type="OrthoDB" id="514964at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000427">
        <property type="term" value="C:plastid-encoded plastid RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044967">
        <property type="entry name" value="PTAC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36371">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36371:SF1">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="276"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
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        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN06636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="680" mass="80339" checksum="7D5DD5E0E518109A" modified="2016-07-06" version="1">MQSFHIQTPPSLFSLIPPKPINPRKPWKSSNSNFSHLLFPSLKFPAPRAFSSDEFPVDESFLEAFGPKDRDTEDEARRKNWIDRGWAPWEEILSPEADFARKSLNEGEEVPLQNPDSIEAFRMLIPKYRKQKMKDLGLTEDEYLAKQFEIKGEKPEPLTTMWAGPLVVRHVPPRDWPPRGWEVDKEELEFIREAHKLMAVRVDYDNVEKVSSTDTDKLCLDRYKMFLKQYKEWVAANKERLEEESYKLDQDYYPGRRKRGEDYEEGMYELPFYYPGQICVGKVTTLHLYQGAFVDIGGVHDGWVPIKRNDWYWIRHHIKVGMTVTVEILAKRDPYRFRFPIEMRFVDPNIDHLIFNRFEFPPVFHREEDANPDELLRDSGRPPVPRKDPGIKVEEEPLLSNHPYVDKLWQLHNAEQLILDDYEINPEKYKDKNLKDLTDDEEFDEENSVEYTKTYYKKQLLPKMIVKVSAKELDMEAALAERLHNNKLRKEAEKRGEKFKVHKLRRNEEMDEYDFIHWRRSFEEREALIRDISCRQALGLPLKEPGRYVDASRFGKDQYDPDNPLYRYDYWGEPKNSEKSKQDRMTDAHNKSIVGKGTVWYEMSYDDAIKLQERREAQAKLKKSHDEADTAKDQSDEDSDSDDDFDYSILSEPTENESDQPVVNGTQSSRLSSDDGMFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FLJ7</accession>
    <name>A0A166FLJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100359</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FLJ7"/>
    <dbReference type="Gramene" id="KZN07918">
        <property type="protein sequence ID" value="KZN07918"/>
        <property type="gene ID" value="DCAR_000587"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205473"/>
    <dbReference type="OMA" id="HITAGCV"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000048">
        <property type="entry name" value="tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000040">
        <property type="entry name" value="Tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF11">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="50"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000517-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="425" mass="46965" checksum="67FBB43AD8839124" modified="2016-07-06" version="1">MENGSKLSSNSKKWGFKKDTKQMTKASMTTIRGVITMLQENLDKDDVRSVVPLSHGDPSAFPCFRTTPVAQDAIAEAVRSAKFNGYSPTVGIPPARIAVAEYLSKDLPEKLSPDDIYLTVGCTQAIEIILTVLARPNANILLPRPGFPYYETRAAFSHLEVRHYDLCPETGWEVNLDEVEALADENTVAVVVINPGNPCGNVYTYNHLKKVAETARKLGILVIADEVYDHLVFGSNPFVPMGVFSSITPIVTLGSISKRWLVPGWRLGWLVTNDPNGILKEQGIVDCIKGFLNISSDPATFIQGALPQILGDTKEDFFLETINSLKMNADFIYSRLQEIPCIVCPSKPEGSMFVMVNLNLSLLDDIKDDMDFCVKLAQEESVMILPGIAVGLKNWLRITFAIEPSAIKEGLERVKAFCDRHAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G222</accession>
    <name>A0A166G222_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166G222"/>
    <dbReference type="Gramene" id="KZN08452">
        <property type="protein sequence ID" value="KZN08452"/>
        <property type="gene ID" value="DCAR_000998"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="69" mass="7665" checksum="E5E0A2972CE9B1A4" modified="2016-07-06" version="1">MVHGNGRANGRPLEASHSALNVVKAGKANELCGKESTTECKGKHKRDAREEEARHVFENQDYIYTQSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GB07</accession>
    <name>A0A166GB07_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GB07"/>
    <dbReference type="Gramene" id="KZN08760">
        <property type="protein sequence ID" value="KZN08760"/>
        <property type="gene ID" value="DCAR_001416"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223244"/>
    <dbReference type="OMA" id="CSAADMN"/>
    <dbReference type="OrthoDB" id="549353at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08824">
        <property type="entry name" value="LOTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10910">
        <property type="entry name" value="PIN_limkain_b1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024768">
        <property type="entry name" value="Marf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379:SF6">
        <property type="entry name" value="EMB|CAB71880.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379">
        <property type="entry name" value="LIMKAIN B LKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="970" mass="108883" checksum="029F8E6AC6B967FD" modified="2016-07-06" version="1">MQPLHPKSLVCLSKLDLLTFSRIPRSPFFRISQFSSTPSPQFHSHSHYPSRRYEEESRLLRVSVWWDIENCTLPAGTNVFRVAQNLTAAVRRNGIKGPVQITAFGDVMQLSRSNQEALSSTGINLTHVPRGGKSSADRSLLVDLMYWVSQNPPPAHLFLISTDGDFANILHRLRLSNYNILLSSSENAPVVLRSAASIMWLWKSLLKGEDLDGKHFNQPPDGPYGSWYGHYKLPLDNPVAVTAQPVARADGLSDAEPKLRPVPKTVVKQIRDVVNLYPEGISITDLHSELGKNNVPLDKDWYGYKEFSCFILSNSHILKIQSWGESQLSVRSIAPTGEGILGIHAETVTNDKDLKKNIPENINYSESSSSRVEDKTSFPISSNANVKEPIRNLKESRKQVKESVKQVLVTSPLVEKVNGTEIIKENLMKSEELKNVEGPQEVVPNPPLVAENEKCAEVSMSNLEGQSPDSKIGFLRTIWGKWLHGENTASANKNVEPEDLTTLNEDNKRIEDMKKECVESTCQCIDAIQRSPKVSATEQTIIDENGATSCETTRGPGILNKMIRWWRGPESDNSTKLSSDNVTTTKVDDNEQGVFTNSFWDDMVAFLQTSSGSNLVMRSKSRKEMAWNLQNQGPAVLRYIIDYDCVRLVDLLISEKKWVEENPSQKYPFKLNLSFESEFNKLNGVKKISVDTQSQAESEKLPADGENTNVPPAVQEKPINPRAQIIQDCQKLVDYIVKTYPEGYHIRCFKNLFLQRYGYSLDVNKLGYLNLRSLLQIMVGVKIDASHIKPDIRLSKVMKFFDQKNTSSSVPVSKSVKSRNLDFPWEELGPVCHDSSKRNEMERSSRIKKEAVIRPVDHNYDTLSDDEFSNTEAENSYLSGLKGQEQPRKIVKHNSLLEILDTWHNGKEDNNARSLLIDVNDMVNDGSRKGRGGNFGQKPQSGKSYSFVADQGRGGLMKSGESAAESRVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GYI8</accession>
    <name>A0A166GYI8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_002157</name>
        <name evidence="15" type="ORF">DCAR_0102209</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GYI8"/>
    <dbReference type="Gramene" id="KZN09501">
        <property type="protein sequence ID" value="KZN09501"/>
        <property type="gene ID" value="DCAR_002157"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204572"/>
    <dbReference type="OMA" id="MHANIDC"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000024">
        <property type="entry name" value="probable protein phosphatase 2C 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="59"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN09501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG83035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="481" mass="53304" checksum="CD9B99E36ADEB5ED" modified="2016-07-06" version="1">MGSCLSTESRGPMPSSPGVRKKKNSRKKLGSRNSSFESQREEQLHRIPGRMFLNGSSDVASMFTQQGKKGTNQDAMIVWENFASRTDTIFCGVFDGHGPYGHLVAKRVRDSLPLKLSAHWEVNTKSDDVLREISLNGTGSLNSESTSFLNADVESRASFDVEENEKNTNMFQTLKETFIKAYKVMDRELKMYTTIDCFCSGTTAVTLIKQGHDLVISNVGDSRAVMGTRDENDLLTAIQLTVDLKPNLPAEAERIRKCKGRVFALRDEPEVARVWLPNNDSPGLAMARAFGDFCLKDFGLISVPEISHRRLTEKDEFVVLATDGIWDVLTNKEVVDIVAAAPARSYAARALVESAVRAWKSKYPTSKVDDCAVVCLFFNSNDLSSASVIESNEKIVPVEECVIDNVKKDTSANIKRGEKLGEECEEEEVEADEDDPLLYADPEKDWSALEGVSRVNTLLTLPRFTPGKEDKPAAGEPKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HCZ2</accession>
    <name>A0A166HCZ2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_002755</name>
        <name evidence="3" type="ORF">DCAR_0102890</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HCZ2"/>
    <dbReference type="Gramene" id="KZN10099">
        <property type="protein sequence ID" value="KZN10099"/>
        <property type="gene ID" value="DCAR_002755"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN10099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="71" mass="7490" checksum="BF788E3D578A7CB4" modified="2016-07-06" version="1">MAGKDGGSNNRGNNNRKKNNESLVLPPRTHVINMVGARLAQSVTALVNNKKNKISRENDDDNAAASASASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HRY9</accession>
    <name>A0A166HRY9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0103127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HRY9"/>
    <dbReference type="Gramene" id="KZN10320">
        <property type="protein sequence ID" value="KZN10320"/>
        <property type="gene ID" value="DCAR_002976"/>
    </dbReference>
    <dbReference type="OMA" id="WVKNRSA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF55">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="704" mass="76903" checksum="A600AE0611AD8841" modified="2016-07-06" version="1">MAKTLNCKSLYIRLLTGLFLLTLFPLAIVSEESDIPQQCGRDAHKCGSLDVPFPFFVNRPSSSPPCSLPDAFGLTCLQLNNLDPYLFLSIKRTSGDGLKGYQVLQFFPDGILVDFSQTASLSDQSSANLSTSSCVEYNDLKSFGFQGREDYFGISADNVLGLYDCEDSSLCKPDCVNTSTILMPPGCSKNHSNYHAACCYPLSDQSYWHPGDDLSIFSQFGCRGFSSWVVVPYDNTTSNYGHAARKRGVKLEWAFPANTNNTNSSYKGAVASSFCAPNAYIVNATTIASGIRCKCEDGFTGDGFTKGVGCLKSCQVDEKQTHGKGCYTKRRPARKLEVLAGVLTSAFCVASLIALCCLLKRHNRSGTFQPHQDHCESSISFGCRTRLFTYHELEEATKGFADCKKLVSSTTGELYTGVLGNGLKVVVHKVQCGSKGDLMQILSQVEVLSAVSHKSMARVLGCSIDCGDAPLVVYEDPANGTLLEYLHQNRQDNKNGLDWYTRLNIVTETSSVLAFLQCEICPPILHNDFQSGYILLDQEFSARLAGFVLLTGQTGPDHSLGSRGLHHFHRSDVYNLGLVLLEIIVGSTLVPLDIDLQKIRGGKLEEIVDPLLYYHEQPSFVKEQIERVADLATRCLLFGGDGKLSMMDAARELLHIRTSTGTGGGGGSSRRGPALEETFSNSSLLQMMSMSPDSIYLPHFSTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I4R2</accession>
    <name>A0A166I4R2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166I4R2"/>
    <dbReference type="Gramene" id="KZN10744">
        <property type="protein sequence ID" value="KZN10744"/>
        <property type="gene ID" value="DCAR_003400"/>
    </dbReference>
    <dbReference type="OMA" id="MRIFGKH"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="61" mass="6926" checksum="EB59CD03045AD60C" modified="2016-07-06" version="1">MRIFGKLMFPGRFIILSASGMLVLAATTYDIHRSIKNNSTPPTQQEMQELNDYIKSLRPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IIQ6</accession>
    <name>A0A166IIQ6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_003831</name>
        <name evidence="11" type="ORF">DCAR_0104067</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IIQ6"/>
    <dbReference type="STRING" id="79200.A0A166IIQ6"/>
    <dbReference type="Gramene" id="KZN11175">
        <property type="protein sequence ID" value="KZN11175"/>
        <property type="gene ID" value="DCAR_003831"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197935"/>
    <dbReference type="OMA" id="KCAVEYD"/>
    <dbReference type="OrthoDB" id="407275at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007875251" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="71"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN11175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG84882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="529" mass="59534" checksum="E8982E30EBBDC5A4" modified="2016-07-06" version="1">MFLQILLVFSLISVTHAAIDPDYTAFVQCLVKNSSPGDKNMVSSIVYTPQNSSFASVLQAYIRNKRFNTSRTLKPTIIVTATKYSHVQSTVICAKNMGIQLKIRSGGHDYDGISYVSDEKFIVLDMFNFRKIDVDIESETVVVEAGAILGELYYKIWEKSNVHGFPAGVCLTVGVGGHLSGGGYGNMLRKFGLSVDHMIDAKIVDVKGRILDRRSMGEDLFWAIRGGGGASFGVILSYTIKLVKVPRVTTVFQVTRTLDENATDIVHRYQFVVDKLDDGLFVRLVLQPEDGKVERMKTVSATFMGMFLGGSTRLMRVMDKGFPELGLKKVDCLEMSWIRSSAWWDNIDNKRSVKELLFLRNSDGVNFLKRKSDYVQTPIPKEGLESMWRKLTELGKVGFVFNPYGGKMNEISSSATPFPHRAGNIYKIQYTANWHEEGPEADKIYMSQIRSLYSFMTPFVSKNPRGAYLNYRDLDIGVSNHGKDSYTQGKVYGQKYFGANFERLVKVKTAVDPDNFFRNEQSIPVKMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IY94</accession>
    <name>A0A166IY94_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104595</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IY94"/>
    <dbReference type="Gramene" id="KZN11620">
        <property type="protein sequence ID" value="KZN11620"/>
        <property type="gene ID" value="DCAR_004276"/>
    </dbReference>
    <dbReference type="OMA" id="FEVTHCN"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF223"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
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    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="7">
        <location>
            <begin position="320"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG85407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="476" mass="54125" checksum="06484E11C90AFAF1" modified="2016-07-06" version="1">MISALTPQKDSDTINVRVTRVWEAINKRNGQVMFTNLILLDEQDNHILATVRNNQKDIYLPLLSEQGVYNISNLKVVPGPPSYRSVDNDLAINFYYKTKIQAAADTGVIPHHKFELKQFKDIPNYVANVANFIDIMGLVINYGELETRTNGAQKMDVTLSNDRQEPIVFTIAHLNMIRNLKFISTCKTNNNRLFISSKTRNERVIVALWESRATNFLDLLPKEGKDPVFVVITGLLAKKYSENVLLSSSDATKTYFNIDYPPLQSMRQAISEENANRGKEMPAPVTLRFVQSENTSAGESNIRDILEAKLPAATTFLRRTCEATIVAVMEGEGWYYNCCPRCARKVQNTEGKYYCTFCSKEAEDFRPRFKLTVRVEDSTAQTTFTLFNKEAEQIVGIPVDKIIDELPEGTNIGEIPPVIRNIIGKRCVFDVKINEYNTVRGYEDYTVFRLKLSDQMERASTSNKDNTDSSKKQRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YCT7</accession>
    <name>A0A175YCT7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_029039</name>
        <name evidence="2" type="ORF">DCAR_0933370</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH13858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM81426">
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        <property type="gene ID" value="DCAR_029039"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM81426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH13858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="90" mass="10058" checksum="9313C19D5270DB42" modified="2016-09-07" version="1">MEFMWDIPDQDAYKINIHCEISDVPSPVGNTVAIASIIRGPTGDKCWGMEGPANNLTLELVNNYEHGLVDELVNLYKEAALKIEREAIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE08</accession>
    <name>A0A175YE08_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933957</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YE08"/>
    <dbReference type="Gramene" id="KZM81869">
        <property type="protein sequence ID" value="KZM81869"/>
        <property type="gene ID" value="DCAR_029482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF50">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH14438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="794" mass="89228" checksum="AD942C5F11510BC8" modified="2016-09-07" version="1">MIIDQIRKHNANVLPDEIFYEIDEDGYTVLEVAVELNQTAVVDLVLDLQHPAAPKRKDGAFISLIPVIYKAQEKGYKNIVDLLTQRYNHRSKLSKDFKDQVSLISAIKSGKTDTVVSLLSDAGRGGQRLVTFVDKLGWTALHHAVYHESVPIIKHIAEAQKGIKPKSGYKDKVPTPFHVAVQRGLTSIVILLMQLWPSTSSTYTAVDKKGQNILHLAALQSKKDMIEGILKNCPAEHKKEFVNKQNNNGYTTLHLLIQLGCFVPELLKYQGLDTSVKNNEDWTPWDMLYVQEQIVVDQILLADPAYQDGRGSRNQGLLRLIYQAMENKYNDKIVELLSATFQTGIDDPNLKGVLDLIIAIQKHDAGPISQLLRGEDNKHFLNFVDSEGWTLLHHAAYHQFDSILSEMIEAQEKVGYQFVCKEGVATPFHVAARCGHTSTVIRLLRLWPASSQYTAVDVNGQNIMQERPAAPSPFTAVDENGQNILHLAALTNKKEMVNGILRCCPPKQKNLLLFKRDVEGNTPLHLLVAQGCFIEDLIKYIAAGDSHTTNNDGWTPRDMLYYRHDIVGDQVQIKMELDGMISTDQSRKLWSSSRKKSTSSSLAVLPASKREKKDILFNEVQKVLMEKKNAKMEEDLQRYREGTNSQIVVSALITTITFTVGFTIPGGLHQSGESNQGLAVLSKKAAFKIFMIADAFALLLSICSLFVYFLENMSDNFEHVTRLHATTLGLNIASIMLTMFVFMTGTYVVFSDSLFLSITIFSIGSLFLIFVILQLLRIAYERRARKNDNYKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YS53</accession>
    <name>A0A175YS53_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_026537</name>
        <name evidence="4" type="ORF">DCAR_0833125</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM86041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YS53"/>
    <dbReference type="Gramene" id="KZM86041">
        <property type="protein sequence ID" value="KZM86041"/>
        <property type="gene ID" value="DCAR_026537"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198739"/>
    <dbReference type="OMA" id="KHHRETE"/>
    <dbReference type="OrthoDB" id="1906668at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33429:SF7">
        <property type="entry name" value="OS02G0708000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33429">
        <property type="entry name" value="OS02G0708000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM86041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24258" checksum="79E3132AA363775B" modified="2016-09-07" version="1">MSQPVLQVYSPPFPTQVMTRPAAAHHSHESFGTVFIVLAVIVVISAIACVLGRICSRRQGHPKGAYRSRDKEPRQNHHSQPKEGDIEFGFDKRFSSSKVAANEDFGGPNVKFGFDKRFPSGKGPANEEPRGPQAFHNGADGGEGGAQFGFDKRFPSGREATNEELRGPTTFHSAATSRGEGSIEFGFDKRFPSGKGPANEGIRGHKPIHNGQSKRVTRFAGDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W2E0</accession>
    <name>A0AAF0W2E0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0100967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W2E0"/>
    <dbReference type="KEGG" id="dcr:108217518"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF15">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="2">
        <location>
            <begin position="183"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="377"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59512" checksum="D5480EAB98B0C1AF" modified="2024-05-29" version="1">MLSKWNFRWNSERQKSWNVWMRGKYGISAHESMTSTQHLNKLSESMKAIKKASEFPGLREKLSQDNFAWKLKNGKTILFWEDRWSGKSSIKDTHPKLYKLSTLKEISVSEVFECWKGNDISTSCQFWSRPLRPWEMEEASKIGDLVRTITLAKDKDEMIWAYTKKGLSLKAATDLLTCNSSPISWEFVWKLKVPNKVKIFLWKAHLGILPTRNFLALRNIKLSDSTTCPVCKLEGETTGHLLIECKMAKAVWTQMFSWWQINPVPPHEMSLMSIWQQSKQFSTRKARITWKVCVSAALWCIWLVRNQVVFRNMEVKMQSAISYFKQLAKEWCLANNLILQNAATWWSVNPMGVLTSSERAQLSNIMQVEEDLIGFTDGSCKTRGEFKFAGIGGNILNKKGNVIFSFSGPSSATTSFEAESQALEFLVKSFSNSDYKASNMVIYSDCLKLVHKVLEVTTAHCTDEKQDLREIFKGLKIRCAFIPSELNSEADFLAKKGAKSKHLTSCWITNCSGPFSQPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W498</accession>
    <name>A0AAF0W498_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0101987</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W498"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000023">
        <property type="entry name" value="Aldehyde dehydrogenase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000019">
        <property type="entry name" value="probable aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF7">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 12A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="69"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG82819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="554" mass="61409" checksum="BB8C37E455D56C40" modified="2024-05-29" version="1">MYRLRVCTKLAAKDPRKTLNCLSSLQSLRSKHSLPFATVDVDSLSGSQPAEVQNLVQGNWTKSSKWNTLVDPLNGEPFIKVSEIDEGGIQPFVDSLSKCPKHGLHNPFKAPERYLLYGDITTKAAHMLSLPEVSDFFTRLIQRVAPKSYQQAHGEVYVTQKFLENFCGDQVRFLARSFAVPGNHLGQQSHGFRWPYGPVAIITPFNFPLEIPVLQLMGALYMGNKPVLKVDSKVCIVMEQMLRLLHECGLPMEDVDFINSDGSTMNKLLLEGKPRMTLFTGSSRVADLLAVDLKGRIKLEDAGFDWKILGPDVQEVDYVAWVCDQDAYACSGQKCSAQSMLFMHENWSQSSLVKQLGNLAARRKLEDLTIGPVLTVTTERMLEHLRKLLKIPGSKLLFGGEALANHKIPSVYGAIKPTAVFVPLDEILKDGNYELVTTEIFGPFQIVTEYKDNQLAMVLDAVERMHAHLTAAVVSNDVLFLQEVIGSTVNGTTYAGLRARTTGAPQNHWFGPAGDPRGAGIGTPEAIKLVWSCHREVIYDIGPMPTNWKLPAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W688</accession>
    <name>A0AAF0W688_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0102929</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CONSTANS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W688"/>
    <dbReference type="KEGG" id="dcr:108204696"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="25"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="69"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="CCT" evidence="11">
        <location>
            <begin position="332"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG83751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="376" mass="42076" checksum="A97789AC29B0764E" modified="2024-05-29" version="1">MSALSRISSGLVQETKKAKTQEEETKNRLCDFCGEDSKAVLYCRADAAKLCLRCDFQVHSTNQLFTKHSRYLLCDSCDASPCSIFCSNHGLVLCQNCDWESHGSGSVLHDRRPLDGFSGCPSVTELLATFGIEDLGQRKKKSCGFDDYLIWDTPCIVGLDDLIVSDGSDHNFVATGVPPLPKNRNAVCGQHKEEIISQLRQMVKLEPNFGDDQFDIELFSGFQSSAFKDNCQQKDACFDFEHNMEATYLPSNAAGALKYCSVSSGEGEDLPSIPLQNFFSTDCKVPDGKPESVQSLNQGNSGHGTQVDASVNVSVPQIFPKVAPREFSSQERDNALTRYKEKKKCRRYEKKIRYESRKIRAESRERIRGRFAKKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6U7</accession>
    <name>A0AAF0W6U7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6U7"/>
    <dbReference type="KEGG" id="dcr:108204664"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000836">
        <property type="entry name" value="O-methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000294">
        <property type="entry name" value="O-methyltransferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="129"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39082" checksum="57F1E7093EF83C7B" modified="2024-05-29" version="1">MAEITNGEVPNECDEIAQAQVDIWRFVFGFAEMAVAKCAVELGIPDILENQGDPMTLSQLSTRLSCSSTALFRVMRFLINRGIFKETVTKEGSTGYVQSPLSCLLTKDGNDSLAPLLLFQNSPVIITPWHFLSACVLDNNKSAFAYAHGKDTWKYCADNQDHNKFFDEAMACDTRRTVPAVLRGCPEVFTGLSSVVDVGGGDGTALRILIKTCPWIHGINFDLPHVLSLVPECEGVEHVGGDMFISVPKANVAFLKWVLHDWDDEKCIQILKNCKQAISEYGEAGKVIIVDAVIDVRGGDKLKDVGLMLDMVMLTHTNMGKERTAEEWGYVLSEAGFSSHTIKNIEAVSSVIVAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W762</accession>
    <name>A0AAF0W762_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0102063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W762"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055566">
        <property type="entry name" value="ARM_LIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056512">
        <property type="entry name" value="LIN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549">
        <property type="entry name" value="OS04G0584500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549:SF1">
        <property type="entry name" value="OS04G0584500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23568">
        <property type="entry name" value="ARM_LIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23628">
        <property type="entry name" value="ARM_LIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="U-box" evidence="7">
        <location>
            <begin position="694"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG82893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1165" mass="130466" checksum="5BE8331E37E4FDE3" modified="2024-05-29" version="1">MAMSLEDLLAKEGFKSRKLKSISRTSTSSEPTSRPLYTNYDNNKSGLSPGATRARRTEKTKSDIPRLQSDLIKDVRAIPRDDLFRKEVLDENLKKEAMLKLNRKNSLQFRGSKSFDIRRAAIFPESEIVEADLDHHEVDTGSQHDERYADIYSNEVFSPSGSTGKVSNGSGGIVTARNMSGKNLQDYKRRGNNSNQNLLGHVSSRLNNHKTMKQTEASSYTPDGGSQKSKSSKESRHRRQPETEEAPAEPALDEIAVRAMISILTGYIKRFFIDEDFKASLRHNCFASLNFVGLEEGINTESKVIDNLEQAIETVERAAEESATATELKKASLHLSVITGLNANDLKDGFTSGIPNSKLSACAHLYLSVIYKLQKKDRIAAKHLLQVFCDSPFPARITLLPELWNDIFFPHLSHIKAWYNHEAVSLADAPSKEIKLKILEKVYNEIMDSGTYQFAAYYKDWLTEGVEAPTIPSIRIPSASIQGVNGGVSNSNYSDPGNTLIPFSSQPMLSLKLYHSDLGSFKKSGADQVVDFEEAENFDISRRSSTASVVEDKETLAYSSNAADHKELVIQQKIAHHEDGLTSCTRSGSGEINYIDKFDDAYQLQVTNSIDMLQELPYTKVNELTIKKLAKAVFSLPLTEECRNEINSSLLTNKDAHCYNVFYESEVQANHPMLFHQGWSENSDSFVHRPSFLSIPEDFICPLSGLCFEEPVTLETGQTFETEALRQWFNKGHKTCPISGVTLEYQAVPLTNFILKRVVDTWKFEHCRTVLGFASQLAVNIEDKFKDEAAVSIFEHLLSFSSRDERIKNARLLISLGGLEFLIRRFGSGNLEEKTIIAALLSHCIEADSGCRNHIARNIEKTCFVELLHCKQAKSIANAVSLLIELLFLNRRMDVCSFLSGLENKELVNTMHVILIYLQSCPSDRRPLVAVLLLHLDLLIAPKKYSIYREEAVDAIVVALESSLTDDKIRENCCHALLVLGGRFSLSGKVMIEDWILKIAGFIDCHEPELLDSNEDLRAANCTLPDTDEDQANEEWLRILSASLLDEKKSFLEKISMCLGSGQLDLVRTCLTTVAWMSSSIASLPDFRRSAFSPLISHLKGVLENSERVEHKVLVSMSLLNFSTIPECRIQLAAIAQELESPLRSLAEVTWTAEKLYITLFGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W776</accession>
    <name>A0AAF0W776_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0102068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W776"/>
    <dbReference type="KEGG" id="dcr:108226978"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="627" mass="69859" checksum="4147B2866DE5C931" modified="2024-05-29" version="1">MFSHFPSSVSHSLSTTTALSLSHHLRPLSLPPSLSKPLFRIFHITPSTFLKNSSLNSSKMDNPEVAIQSVADDFNKQSLGEAKLKLEDLNWDHSFVRELPGDPRTDIIPREVLHACYTKVSPSVEVENPQLVAWSESVADLLDLDPGEFERPDIAHKFSGASPLAGGMPYAQCYGGHQFGMWAGQLGDGRAITLGEVLNSKSERWELQLKGAGKTPYSRFADGLAVLRSSIREFLCSEAMKSLGIPTTRALCLVTTGKNVSRDMFYDGNPKDEPGAVVCRVAQSFLRFGSFQLHASRGKEDLDIVRTLADYTIRHHFPHIENMSKSESLSFSTGEEDNAVVDLTSNKYAAWTVEVAERTASLVASWQGVGFTHGVLNTDNMSVLGLTIDYGPFGFLDAFDPSYTPNTTDLPGRRYCFANQPDIGLWNIAQFASTISAAKLINDKEANYAMERYGTKFMDEYQAIMTKKLGLPKYNKQLISKLLNNMAVDKVDYTNFFRILSNIKADVNIPEEDLLVPLKAVLLDIGKERKEAWTSWVQTYIQELVESGISDEERKTSMNSINPKYVLRNYLCQSAIDVAESGDFGEVRRLLKVMEKPFDEQPGMEKYARLPPAWAYRPGVCMLSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7T3</accession>
    <name>A0AAF0W7T3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">DCAR_0104060</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7T3"/>
    <dbReference type="KEGG" id="dcr:108223579"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF592">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042053053" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="21"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="651"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="707"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="736"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="WOG84875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1025" mass="111554" checksum="74DDE2CA8AC15DEA" modified="2024-05-29" version="1">MIFPCFAILVILTISATSDCNYNAAATFLSNVTDRQALLSVRTYITGHPPVLSSWNNSIHFCHWEGVICSRRHQRVMVLNLSSQQLEGTLSPHIGNLSFLRAIFLDGNTLRGFIPVEIGKLFRLRHLYLGSNFFQGRFPMSLSYCLELKSISIPKNSLKGNLPEFTSWSKLTSFNALKNNFTGPIPSSIGNMSSLQFLDLGGNFLTGSIPLEVSDLSKLEYLQLAGNNLSGKVPVQLYNISSLSSFSLAINELLEGNLPADLGSTLPNLQVFFAGGNSFSGPFPPSITNASQLVLIDIAYNYIVGPLPMNLGSLRSLEEINLGDNKLGINQQPGDLGFLDSLVNSTGLEYVGLNENGFSGEIPSSIVNLSTTLDGLSLYGNNIYGSIPREIGNLLNITELSLSENMLTGSIPASICKLSKLGSLSLGNNNISGVIPACIGNITGLLLLSLESNMLHETIPPSLFNISSLQELRLFSNLLSGMIPEHIGLSSLSRGLFLQQNLLTGPLPSNIGSLIHLVKLRVSDNRLTGVIPASLRDCVMLEELLMEGNLFDGSIPSSLKGLRSLAFLDLSNNNISGNIPRFLGELPQIQFLNLSHNKLGGDVPKKGLFSNVSAFSIVGNFQLCGGIQALKLRACPANISKTKEKQFPLRILPLIIVLPLAALFAFFAFGFYQIKKSKKKNAPILVLPEEKYPRISYQDLLLATDNFSPNNWLGGGKYGSVYKGIMDSSQQNIAVKVLNIEVHGANKNFLVECETLRIVRHRNLIKIITACSSTDFKGNDFKALVFELMKNGSLDNWLHPSLSNQETERNLTLLQRLDIAIDVASAVDYLHHQCPTKILHSDIKPSNILLDEELVARVGDFGLARILLTSTGDINNQAQSNSNSTGVRGTVGYVPPEYGMGGKISAEGDVYSYGILLLEMFSGQRPTSILMENANDLHDYVKKALPHGVMKITDPRIVIQREDGGSHGGDIVAKSGISSKMEVCLASIFEVGILCSVEIPKERIDIGIAVKLLRVARDKLLKHGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9D4</accession>
    <name>A0AAF0W9D4_DAUCS</name>
    <protein>
        <submittedName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="95" mass="10726" checksum="B549D5E59EF2CEA3" modified="2024-05-29" version="1">MPRSRINRNFIDKTFSIIANILLRIIPTTSGIKEAFTYYRDVFETSGCKIEAELINDQLTSRGSSNTSLGNFNVNISVIEFYLLKCLGELPGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WCC6_DAUCS</name>
    <protein>
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            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0205402</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="57671" checksum="6A55659F5BA3FB6E" modified="2024-05-29" version="1">MDLMDLYILVGVSVLLIVLPKLLQNYKSKLPHAPGPRGLPFIGNLHQIDVSILPHYLWKLSRQYGPIMYLRLGYLPVLVISNSEMAKLILKTHDLNFCSRPSLFGLRKVSYNYRDVVFSPYNEYWREMRKIVTIHLFSSKRIQSFRSIREEEVFLMIKGISEKASSNEVLNLTQILVPLANTIVCRLAFGKKFDEASMKRFEVLLARFQAVTAAFYFSDIFPMFARLDRLTGSSAELDRFFMDMDLFYQELIEEHLVASRPSSMEGDVIDILLQLKRDDQLSSIDFTIDNIKAIIMNIFFGGTETTANTLIWIMTSLIKNPRVMRKAQEEVRKLMHGKSRIDEEDLQSIKLPYLEAVIKESMRLDPVVPLIPRESLDRLVINGYEIKPKTIVYVNALAIGRDPQVWENADEFLPERFMDRDIQFKGHDFEYIPFGSGRRNCSGMYMAVAILELGLSNLLYSFDWELPPGIRKEDIDTEIGQGVTMHKKNALYLQAKNYC</sequence>
</entry>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
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    </dbReference>
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        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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                <person name="Macko-Podgorni A."/>
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    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34995" checksum="94C632989884A9A9" modified="2024-05-29" version="1">MKKPMLKKRLKRHDSTVAPPTNSLHISNTPDSPSMKLENTQTRGKEKLFVTVLKIFFLVVVALVNDRTVVSVSMSAFSLFFLEHVVNYFYRLFKYLFVSQTMKKTASFSSNEGDTSASDSVVSDCSSLNLSSEEMQDVQAAFNSDFDHDSSQSILENDESCNGEGPSTKHRANRKEKVKEKIKKLVPKKLRRSMKKRSGSKPEDPGPEEEFNFDLKENKTEHDDVKEDISVAATDSYQSMISDSEESVVEAEPQRATDRRTLVLEYWLVSFIVLAGLVEGQSLAIVLTVAWCLIVKLVGRMRMKKWKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGT1</accession>
    <name>A0AAF0WGT1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0207037</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGT1"/>
    <dbReference type="KEGG" id="dcr:108208654"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000005">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF7">
        <property type="entry name" value="AMMONIUM TRANSPORTER 2 MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG87805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="454" mass="49680" checksum="22E66C7A9E2DA759" modified="2024-05-29" version="1">MSNFTLPLNLAPNESSPEWMNKADNAWQLVAATMVGLQSVPGLIILYGGAVKKKWAVNSAFMALYAFACVLVCWVCWGYRLSFGEKFIPVWGQVNVALNQEYLLGPAFSGAFPVSTMVFFQFVFAAITLILIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSLEGWLALKGIIDYSGGYVIHLSSGVAGFTAAYWVGPRLTKDRERFPPNNIILMLAGAGLLWMGWTGFNGGDPYSVSTDASLAVLNTHICAATSLLTWLFLDIIFFRKASVIGAVQGMITGLVCITPAAGVVEGWAAIIMGICSGSIPWFTMMVVHKRSELLQRVDDTMAVFHTHAIAGCLGGILTGLFAKPSLCNLFYAKYAHYVGLFYGFREGRVHAGFRQIGIQLLGILFVVVVNVVVTSLVCLLVQLIFPLRMSDEDMEVGDDAIHGEEAYAIWGDGEKLDAASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJG6</accession>
    <name>A0AAF0WJG6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0208631</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJG6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000134">
        <property type="entry name" value="Casein kinase I isoform delta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="304"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="421"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG89393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="465" mass="52095" checksum="C39F90AC7182FAC1" modified="2024-05-29" version="1">MEPRVGNKFRLGRKIGSGSFGEIYLGTNIQTNEEVAIKLENIKTKHPQLLYESKLYKILQGGTGIPNVRWFGVEGDYNVLVMDLLGPSLEDLFNFCSRKLSLKTVLMLADQMVNRVEFVHSKSFLHRDIKPDNFLMGLGRRANQVYAIDFGLAKKYRDSTTHQHIPYRENKNLTGTARYASMNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGNKKQKYEKISEKKVSTSIEALCRGYPTEFASYFHYCRSLRFDDKPDYAYLKRIFRDLFIREGFQFDYVFDWTILKYQQSQLANPPSRALGANVGSSSGMPPAAANVNRQSGGDEGRHAGWSSANPSRSRSTGLPFISGSLSKQKNPIPDEVSCLPNSNNMRTSGSSRRPGVSGSREPAISGDFDSVRARATDASPGTVRKISSSAQRSSPVVSSDQKHSTSGRNKNLESSLKGIEGLHINDERLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WK21</accession>
    <name>A0AAF0WK21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0209032</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WK21"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="15"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG89793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="328" mass="37415" checksum="E989FC417617A02F" modified="2024-05-29" version="1">MPIRYSYSDIKKLTNNFKDKLGEGGFGTVYKGKLRSGLLVAVKILANSKATGKEFINEVATSGRIHHVNVVELLGFCFEGPKRALIYEFMPNGSLEKYIYSKEGTEEEVVPLSWEKIYKISYKVATGIDYLHRGCDMQILHFDIKPHNILLDENFNPAISDFGLAKLYATDHSIVTLTAARGTMGYMAPEMFYKNIGGISYKADVYSFGMLLMEMAGRRKNLNPFVDHISQIHFPSWVHDRFSEGKELEMEDVTEEDRKLVKKMILVALWCIQMKPSERPSMNRVIKMLEGDIEQLVMPPKPFLYPQEDTTEIDNPIHSSPNLLPDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKX9</accession>
    <name>A0AAF0WKX9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7032 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0209705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKX9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054296">
        <property type="entry name" value="DUF7032"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46043:SF9">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23005">
        <property type="entry name" value="DUF7032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF7032" evidence="3">
        <location>
            <begin position="11"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG90461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="548" mass="59211" checksum="97159BE45703B0BB" modified="2024-05-29" version="1">MEQSAEEWLAHAQENVPIALGKAREVKGFSGRWKVIISKLELIPSRLSDLSSHPCFSKNTLSTEQLQAVAKTLDEAIELAEICVKEKYEGKLRMQSDLDSLSGKLDLNLRDCGLLIKTGVLGDVTFPSTASNPTDSETATHFSIKELLARLQIGHLEAKNKAIDSLVEILQEDEKNVLAVLGRSNIAALVQLLTATSPRIREKTVTVICSLAESGSCESWLVSEGVLPPLIRLVESGSAVSKEKSSVSLQRLSVSTETSRSIVGHGGVAPLIDICRSGDSVTQAAAACTLKNISAVPEVRQTLAEEGIVKVMINLLDHGILLGSREYAAECLQNLTSTNDSLRRCVVAEGGIQSLLIYLDGPLPQESAVGALRNLVSSVSEEILISHGLLPVLVHVLKSGSLGAQQAATSAICQICSSIEMKKMIGEAGFIPLLVKMLEAKSNSNREVAARALSSLMNLSHNCREVRRDEKSVPNLVQLLDPSPLNTAKKYAVTCLALLYSSKKCKKLMISYGAIGYLKKLTEMDIPGAKKLLERLERGKLRSLFSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WL97</accession>
    <name>A0AAF0WL97_DAUCS</name>
    <protein>
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    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        <location>
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        </location>
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            <begin position="1"/>
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    </evidence>
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        </source>
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    </evidence>
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        </source>
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    <sequence length="352" mass="39664" checksum="8F4CC777EA193011" modified="2024-05-29" version="1">MNSDGTSSTSGATGSGDSATPRRNSKRPKYSKFTQQELPACKPILTPGWVISSFLLVCIVFIPIGLASLFASRSVVEIVDRYETACIPGGFRKDKVKYIQSDSNKTCRRNLTVNKDMKPPIYVYYQLDNFYQNHRRYVKSRSDPQLKSKSKENETNDCKPEATINGKAILPCGLIAWSLFNDTYSFSRSSQKLQVNKKDISWKSDREHKFGKDVFPKNFQNRTVKGGATLNASIPLSQQEDLIVWMRTAALPTFRKLYGKIEVELKKGDVIQVTLENNYNTYSFNGKKKLVLSTTKWIGGKNDFIGIAYLTVGGLCFLLAMSFTIIYYVKPRRLGDPTYLSWNRNPGGGANN</sequence>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
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    </reference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
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    <feature type="domain" description="MADS-box" evidence="6">
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            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="63" mass="7186" checksum="76AEA5C23C781F0E" modified="2024-05-29" version="1">MGRVKLQIKRIENNTNRQVTYSKRRNGLMKKAFEISVLCEVDVGLLMFSPSGRVSVFAGNNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLZ7</accession>
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    </protein>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <comment type="subcellular location">
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        <property type="entry name" value="PUP_plant"/>
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        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        </location>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="14"/>
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        </location>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOG92736.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase SIS3"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="SIS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47179">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47179:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="236"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG95288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="372" mass="41990" checksum="40C537CAEE29ECAC" modified="2024-05-29" version="1">MAIRGVDFKWYDGFFLSMLATSIIIVAINWKRYHYCEYPLHIWIVVDYTTVFVFRLLMFVDNGLASGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLWAWTIIGTLWFTSARSCLPEEGQKYGFLIWLLFSYCGLICIACMSIGKWITRRQAHLLRGAQQGIPISQFGVLVDMIRVPDWAFEAAGQEMRGMGQDTAAYHPGLYLTPTQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVECIDEWLRLNVKCPRCRCSVFPNLDLSALSSIRADSDHSPAAVVTAAPYVRTQPPSQSYLLRLQGLLRQVGTENGGNADLALEAAENGVLPVLMQPNRSPEPGRMRSSSLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYJ4</accession>
    <name>A0AAF0WYJ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_0416686</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYJ4"/>
    <dbReference type="KEGG" id="dcr:108218964"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042243652" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="22"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="294"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG97346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="617" mass="68200" checksum="BFB71AF939945A06" modified="2024-05-29" version="1">MAAWDLVGLILVLHHVLMVAANMEGDALHNLRTSLQDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNAALSGQLVPLGQLKNLQYLELYSNNISGPIPSDLGNLTNLVSLDLYMNSFSGPIPDTLGKLTRLRFLRLNNNSLSGPIPMSLTNITTLQVLDLSNNRLSGPVPDNGSFSLFTPISFANNLNLCGPVTGRPCPGSPPFSPPPPFIPPSTVQPPGQNGPTGAIAGGVAAGAALLFAAPAMAFAWWRRRKPREHFFDVPAEEDPEVHLGQLKRFSLRELQVATDTFSTILGRGGFGKVYKGRLADGSLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERQPSEPPLDWPTRKRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLARLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKSLLKEKKLEMLVDPDLQNNYIDTEVEQLIQVALLCTQGSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEVIHQDVELAPHRTSEWILDSTDNLHAFELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X109</accession>
    <name>A0AAF0X109_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0417046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000057">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF1">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG99714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="186" mass="20674" checksum="3816CF268E49AA47" modified="2024-05-29" version="1">MEKDIDRILWTEEALTQRVTQLASQITADFASCPSPPILLGVATGAFIFLADLVREINLPVSVDFIRASSYGSSTHSDGAPKFSLDVKLNIQDKHVIVVEDIIDTGNTLCCLVAHLKKKGASSISVCTLLDKPSRRKVNFELVGNGKYYCGFECPDYFVVGYGLDFAERYRNLRFIGVLKPEIYKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X589</accession>
    <name>A0AAF0X589_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="13">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0520066</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Possesses 5'-&gt;3' exoribonuclease activity. Acts as an endogenous post-transcriptional gene silencing (PTGS) suppressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="13"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X589"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010587">
        <property type="term" value="P:miRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="16">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="820"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="835"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH00692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="964" mass="110343" checksum="7CD5975144B55110" modified="2024-05-29" version="1">MGVPAFYRWLAEKYPMVVVDVIEEEAVEIEGVKIPVDTSKPNPNQLEYDNLYLDMNGIIHPCFHPEDRPSPTSFTEVFQCMFDYIDRLFVMVRPRKLLYMAIDGVAPRAKMNQQRSRRFRAAKDAADAAAEEERLREEFEKAGRKLPPKQESQVCDSNVITPGTEFMAVLSVALQYYIHLRINNDPGWKSVKVILSDANVPGEGEHKIMSYIRLQRNLPGFDPNTRHCLYGLDADLIMLALATHEIHFSILREVVFTPGQQNKCFLCGQMGHMAAECEGKAKRKNGEFDEKCEIVPKKPYQFLHIWTLREYLELEMRIPNIPFTIDFERIVDDFIFMCFFVGNDFLPHMPTLEIREGAINLLLAVYKKEFRALGGYLTDGSKPNLSRVEHFIQAVGSYEDKIFSKRARLHQASDNKEELKTKLKGILREKSDAFNSENPEEDKVKLGEPGWKERYYEEKFSAKTPEELDRVRRDVVLSYAEGLCWVMHYYYEGVCSWQWFYPYHYAPFASDLKDLGQLNISFELGSPFKPFNQLLGVFPAASSHALPQQYRKLMTDPNSPIIDFYPADFEVDMNGKRFAWQGIAKLPFIDEARLLAEVAKIEYTLTDEEVRRNSTMYDMLFVALSHPLSPYIFSLDDRCKQLDDKERTQIKEQIDPGASGGMNGYISLCAGDPCPTIFRSPVEGMEDIMDNQVICAIYILPDAHNHIAHPPPGVKLPKKTVSFGDLTPEPVLWHEDSGRKPWENKRNNPPGAISGRQLGDAAHRLVANSLQIKKDRNVDGDHYGHPPYVTTHGPAFHPNQTLRHHGQEQYRMVPPAYPEGRHMLPNSVPGHSQYDRSYNQPYASSSTRHPNNWHRPPLDQSRDYPPQVYHSQGSHQNRHVYQPRGPLHAVQAPPPIAAGVPFYQQVGYQAYGGANYHHQNGGPPTVNPRVGRDYGRHQQFGNQFSALDISRGVRRPPPPPGYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X6H2</accession>
    <name>A0AAF0X6H2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0521642</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="6">Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of target mRNAs. Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of CLV1, PNH, WUS and FAS2.</text>
    </comment>
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        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF128">
        <property type="entry name" value="PUMILIO HOMOLOG 1-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07990">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
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        <location>
            <begin position="688"/>
            <end position="1028"/>
        </location>
    </feature>
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        <location>
            <begin position="708"/>
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        </location>
    </feature>
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        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
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    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XBV5"/>
    <dbReference type="KEGG" id="dcr:108227598"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14664">
        <property type="entry name" value="STK_BAK1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042021069" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="29"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="290"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH04945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="614" mass="68243" checksum="47C862D4DC763BF9" modified="2024-05-29" version="1">MDRFVIWSFGAFCLVLVLNQFLSVSGNAEGDALNALKNNLEDPNNVLQSWDATLVNPCTWFHVTCNNENSVTRVDLGNANLSGQLVTQLGQLPNLQYLELYSNNISGRVPNELGNLTNLVSLDLYLNKLNGPIPDTLKKLQKLRFLRLNNNTLTGTIPFSLTTIGSLQVLDLSNNRLTGPVPVNGSFTLFTPISFSGNLLDALPVSPPPPLPPSPPSSKAVNSATGAIAGGVAAGAALLFAGPAIALAWWRKKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVATDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMSNGSVASCLRERSESQLPLEWRIRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDCFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKDKKLESLVDVDLQGNYVDEQVEELIQVALLCTQNSPTERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDYSNAHNQHNDWMIPDSIHNLQPDELSNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMX1</accession>
    <name>A0AAF0XMX1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0830506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMX1"/>
    <dbReference type="KEGG" id="dcr:108200095"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045140">
        <property type="term" value="F:inositol phosphoceramide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF62">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL:CERAMIDE INOSITOLPHOSPHOTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="10">
        <location>
            <begin position="171"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH11028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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        </source>
    </evidence>
    <sequence length="309" mass="35653" checksum="6659F56E37981508" modified="2024-05-29" version="1">MSFYIGREASKLWKRICAETSTELKLLFENWKYLLGGLICQYIHGLAARGVHYFHRPGPILQDVGYYLLPELGIERAYISETVFTFIFASFVLWTFHPFILKNKKIYTVLLWCRVLAFLFGCQILRIITFYSTQLPGPNYHCREGSKFATLPLPDNILEVFVIIPRGVLYGCGDLIFSSHMIFSLVFVRTYQKYGTRRFIKQCAWLTVIIQSLLIIASRKHYTVDVVVAWYTVNLVVYFIDHKLPELPDRSSSALLLPVSKDSRVKEENHKLLNGNSVDQADWRSRTQTNGKVQELANVVHTEPAMNGQ</sequence>
</entry>
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    <name>A0AAF0XQX3_DAUCS</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="32208" checksum="5BEBF7B66028C62C" modified="2024-05-29" version="1">MLNLFLPSRTISSYVLKALHLIEENANSLDDNHQLAVYTPADPAASDGTVTQPPQNQVCRIAPSVEAYTVQYSLYYKWRLIPSKKRFEEIREHLIEKPFVCRALHQRLPQISCTVDLNFQPDGSKLWAIETPNITRPPSGWERILKIKGPQGTRFADVFADMYYYTSSHERLLSTVEVRGIRVNIPMRYPREHPSEVDGADLTQFSFKIPILVKENSLEEHTTLARTRNLSNTNTSLKPLELHKQLVYYSKALITYFKTFVFIFVSISYLFEMSYYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <location>
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        </location>
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        <location>
            <begin position="26"/>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="15723" checksum="D4EAD64783F68142" modified="2024-05-29" version="1">MALKFTVNVLVLLSVTLMAASSVEAQLGYPVSYDITGKLYCTLNGSIGTNGTATPVFPGALVQVVCVNTTNPLLTGTTLADGRFTLQTPNPIPPNCTLVVPTPLSTCNSSLPATGGLISALRSVGSIFIRLYAKHYVYIPEGFSYVPDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSA5</accession>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
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        <property type="protein sequence ID" value="WOH12224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="157"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH12224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="310" mass="35434" checksum="C04F4EE8F61AC199" modified="2024-05-29" version="1">MADRNNGVESLKLPVIDLLSSHRLATATSIHQACKDTGFFYLINHGIEIELIQKALEESKNFFSLPLEEKMKLAWKEHRGYTPLCSERLGDVKETIYIGPVEDDKSYLNQWPSRDILPSWRFTMELYYEKVRAAGRMLISLIALALNLEEDFFEKDGPMDPFLRLLHYPGEQGVLDEKVLGSSAHSDYGMMTLLVTDGVPGLQVCREKTKKPQVWEDVPHISGAFVVNIGDMMERWTNCLYRSTLHRVIRTGQERYSMAFFLNPFPDSVVKCLKSCCSEASPPRFPPIRSGDYLKERYRANNIGNTGNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTR5</accession>
    <name>A0AAF0XTR5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0933718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTR5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487:SF16">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487">
        <property type="entry name" value="OS06G0651300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="365" mass="40843" checksum="6011A9D12D9E46A3" modified="2024-05-29" version="1">MGFFGFYVVLKCFKHLAWPTFALVYPLCASVQAIETNSNCEMRKLVTYWVLFSLISLLDHTFGGLIDWLPVWPYAKIIAIFWLVIPRFDGAGIAYNCFVRPCLSGQLPQVVLVLLNKNQDPSLEEESFPVPAQRDIIEHGGEALEELISTKPKVMKPTSSNADIKTVKILTGTASAIDKASSTMKPNTRVDVKVLTLPAPLVQNVQREWTCAVCQVTTTSEATLNSHLQGRKHKIKCEELKAGKQTTMRVVTIGGTNRVRYCTICQVKTQSEAIWNSHVQGKKHKSKCKELRAIVKAEKNNFSSTATKSCQANQDPNNRFGCDICQMTLQSEVTLQSHLQGKRHKSNSEQFKARKMAEEYAFPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTT4</accession>
    <name>A0AAF0XTT4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0933748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTT4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35490:SF3">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35490">
        <property type="entry name" value="BACTERIOPHAGE N4 ADSORPTION B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="325" mass="37201" checksum="56315512201FDA59" modified="2024-05-29" version="1">MPTFTSIALESLIEPRFRDSYKDSAKKTVKSVKDDDDKEVREEEVKKPSRNRVNISPALYITPEPTPIVYLSSESVSPSPYVANRKGRGGLRRRSEVVVRKEEERVVEEERVVEEDEKVKKEKEMREVAEENFSHDGSMSSTQHSCHSIESEMRATRLSLLDEIGKRKAEDALGLMYSQWQRVADLLLAQSGLRFPAPSNCMQFEIGPVEQFCQELVVTRFVSEALGQGLARAETEIAAEEIFKAKDQEISRLRDRLQYCEAVNHEMSQRNQEVIEVARKQRKKKKTLQKWLWCGLGLSITIGASVVAYSCLQQTSKYRAFADFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUI1</accession>
    <name>A0AAF0XUI1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DELLA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0832568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUI1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="385"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="205"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="52452" checksum="3170E9AAAF0CCCAE" modified="2024-05-29" version="1">MLELFAPEQERSSPTSLSSFRILNKCRSNFEEQNSEKVKVPDSEISCSKVGGQSVIEIIQLAGEKFIKSRSLRIDDLSELSQGPYVDKTEDVEFIIQNLLLSAEKIGEQQFELARSLLNHCNEFCSDKGNPIQRLVYYFSAALRERIDLEMGVTTPKVVSLEFEKAIMNPSSTSLTLHQRVPFCQVMQFTGVQAIIEHVSQAKRIHIIDLNIKYGTHCSIFIQDLASRHACPVEHLRITALGTNSNILPTDTGKRLANFAKSVNLSFSFHVVTVADMLDLHENLFELDTDEVTAVYAPYILWTLISKQDRLECLMKVIRIINPCVMVVSENEVNHNSPMFVNRFVEALFFFGAYFDCFGEYMDADDPGRFSAEYMFGHGIKNIVATDGKERTVRQVKNDVWVSFFAQYNMVQKELSRSSLYQATLVAKNFECEDSCSLKLDGKSLILGWKGTSVLSLSAWEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVN9</accession>
    <name>A0AAF0XVN9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="8">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="8">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0934387</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVN9"/>
    <dbReference type="KEGG" id="dcr:108192273"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04097">
        <property type="entry name" value="mtEFG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000003">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009381">
        <property type="entry name" value="PRK12740.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="56"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="132"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="186"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH14860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="747" mass="82609" checksum="95371C01D9B3B5F5" modified="2024-05-29" version="1">MLRSTRNSTSRLLYTLSNAPPSCPSPITSLLHLRHFAAPVAKVKEDKDPWWKESMDRLRNIGISAHIDSGKTTLTERILFYTGRIHEIHEVRGKDGVGAKMDSMDLEREKGITIQSAATYCTWKDYQVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRYDVPRLAFINKLDRMGADPWKVLSQARSKLRHHSAAVQVPIGLEEELQGLVDLVQMKAYYFTGSSGETITTEQIPGNLEEMVSEKRRELIEAVSEVDDKLAEAFLSDEPISPIELEEAIRRATIARKFIPVFMGSAFKNKGVQPLLDGVLSYLPCPTEVCNYALDQTKNEEKVPLSGTPAGPLVALAFKLEEGRFGQLTYLRIYEGVLKKGDFIVNVNTGKKIKVPRLVRMHSNEMEDIQEAHAGQIVAVFGIECASGDTFTDGKVKYTMTSMNVPEPVMSLAVSPVSKDSGGQFSKALSRFQREDPTFRVGLDGESGQTIISGMGELHLDIYVERIKREYKVDATVGKPRVNFRETITQRTEFDYLHKKQTGGQGQYGRVCGYVEPLPPGSSSKFEFENQIVGQAIPSGFIPAIEKGFKEAANSGSLIGHPVENILVALTDGAAHTVDSSELAFKLAAIYAFRQCYAAARPVILEPVMLVELKVPTEFQGTVTGDLNKRKGLIIANDQDGDDSVITAHVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSIVSNDVQTQLVNAYKTTKGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWG0</accession>
    <name>A0AAF0XWG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWG0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <text evidence="7">Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2+) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases.</text>
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</entry>
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                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis; flavonoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASM5"/>
    <dbReference type="KEGG" id="dcr:108213383"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000016">
        <property type="entry name" value="Naringenin,2-oxoglutarate 3-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="194"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG91472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="357" mass="40258" checksum="13EC4CC8CB34DD3B" modified="2024-05-29" version="1">MAPTTITALAKEKTLNSDFVRDEDERPKVAYNQFSTEIPIISLAGIDDDSNGRRPEVCRKIVEAFEDWGIFQVVDHGIDSGLIAEMSRLSREFFALPAEEKLRYDTTGGKRGGFTISTHLQGDDVKDWREFVVYFSYPVDARDYSRWPDKPEGWRSVTEVYSEKLMALGAKLLEVLSEAMGLEKEALTEACVNMEQKVLINYYPTCPQPDLTLGVRRHTDPGTITILLQDMVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSTSSRLSIATFQNPAQNAIVYPLKIREGEKPILEEAMTYAEMYKKNMTKHIEVATQKKLAKEKRLQNEKAKLETKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AXL0</accession>
    <name>A0AAF1AXL0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0417802</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AXL0"/>
    <dbReference type="KEGG" id="dcr:108218348"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF60">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="661"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="962"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="962"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG98459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1013" mass="111352" checksum="F29EC1CADDFDDC63" modified="2024-05-29" version="1">MEHRFRNQAYVYLLVLGFFFFHDLGCVAQLLPPQEVQTLKAIATKLNIKHWDVKSTSCSGGGGLSVEFNIPQPLSNVTCDCTFSNGSVCHVTHIQLKRLNLTGELPSEFSKLAYLQELELAQNNINGTIPATFGQLPLKTLGLLDNRISGSIPPEIANIDMLEELIIEDNQLGGTLPPGLGNLKSLRRLLLSANNFTGTIPESFSALKNLSDFTIDGSGLSGKIPDFIGNWTKMTILNLQGTSMEGPIPSSISMLKNLRELRISDLNGSISRFPNLRNMTNMKYLILRNCLIEDSFPPYIAEMKSLNTLDLSFNRLSGPIPDSVQPLESSLNFLYLNSNLLSGVIPSWISNSKKNFDASYNNFTQLASQYGCQASRVNLVASHSSSTSDSTLWCLKKDLPCPSSTQYHSLFVNCGGPKIKSGGKEYEDDLVTDGASYFYPTEQWAYSSTGVYIYNDKSNFVASKANVTGEEYYQTARVSPSSLKYYGLCLQKGSYKVRLHFAEIQFSDDATFSSIGRRIFDVSIQENVVWKDFNIAENAGGIGKGFTLERDVTINGSTLEIHLYWAGKGTTAVPDRGVYGPLISAISVTPNYKISTGALSTGTIAGIVVASLVSVALILVVLWRKGFLGGKDSEDKELRGAIEQQTGYFSLRHIKAATGNFDHANKIGEGGFGPVYKGRLADGLEIAVKQLSSKSKQGTREFINEIGMISALQHPNLVKLYGCCIEGNQLLLVYEYLENNSLARALFGRDGQRLNLDWVTRKTILLGIARGLTYLHEESRIKIIHRDIKGTNVLLDKDLNAKISDFGLAKLDEEEHTHISTRIAGTVGYMAPEYAMRGYLTDKADVYSFGIVALEIVSGKSNTSYMPKDEFVSLLDWAYVLQEQGSLLEIVDPILGTNYSKQEVISTLNIALLCSSQSASLRPAMSAVVSMLEGKRKVEPPNIKRTTTNNNMISKGFEKITTESQTQSSTFSQESLGARTGSSMDAPWFDSTISIPSKDTGKTVKDLYDVNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B068</accession>
    <name>A0AAF1B068_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial adenine nucleotide transporter BTL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0418393</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable mitochondrial adenylate carrier that catalyzes the transport of ATP, ADP and AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG99046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B068"/>
    <dbReference type="KEGG" id="dcr:108219496"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000098">
        <property type="entry name" value="Mitochondrial substrate carrier family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="94"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="187"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="301"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG99046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="42759" checksum="0BC2CA437B4BA1D7" modified="2024-05-29" version="1">MSPNRISFKDSLRSPPDIAAGIFLHDAVPSSLVEFITSRTSPSYNQSLHSSSSPPPRRLGDFLSVSLSTKGGNLPDNGEGKKRIVRLRGKRGAINTTKHLWAGAVAAMVSRTFVAPLERLKLEYMVRGEQKKLFELVKMIAATQGLRGFWKGNLVNILRTAPFKAINFCSYDTYRKQLLKLSGNNETTNIERFISGAAAGVTATVLCLPLDTIRTKLVAPGGEALGGVIGAFCHVVQTEGFFSLYKGLLPSILSMAPSAAVFYGVYDMLKSAYLHSPEGRKRIKNMKQQGQELNIWEQLELGPVRTLLHGAISGACAEVATYPFEVVRRHLQLQARENKLSTLATFIKIVERGGIPALFAGLVPSLLQVLPSAAISYFMYEFMKIVLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3P7</accession>
    <name>A0AAF1B3P7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0622901</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="1"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000007">
        <property type="entry name" value="Putative 1-phosphatidylinositol-4-phosphate 5-kinase/ zinc ion binding family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF4">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE FAB1D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="10">
        <location>
            <begin position="1142"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="595"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="962"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="965"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1488" mass="165777" checksum="E02302DB6BE2DE2B" modified="2024-05-29" version="1">MESCFKSAVGTKKMVDYQIVGCCCSVQDEYNHSSVDSLRETSLPPPGNGLLPSNMVEQNSLNGSANFVDGRTGKDDEFMQKANNLPIYEERENNHFNKGEMDLQYWLPPETEDQENDMDCSLANFDDDDDEGDDGTKWGMPSSLGSFGEESSGSFSYKEEKQIAMDKVRNNKFKAVVHHLLKNAGVASSGKDGDNWVDIVTSLSWEAASFVKPDIGEGKAMDPDGYVKVKCVATGSRSESKLVRGMVFKKHAAHKHMPTNYKKPRLLLIQGALDLSLSGLSSFESMNQGKETLKSIMDALDMCNPNVILVEKTVSRDIQESILAKGMTLVIDMKLHRLQRVARCTGSSILSSDTLTGKKLGQCDSFHFAKFVEEHATAGESGKKPSKTLMFLDGCPTRLGCTILLKGTNSEELKKIKLVVQYAVLVAYHLILETAFLLDQRAMYSTLPLDGLVNLLDANQTPCISPREADVAMPEDSVAESDSSGMIDIHISNGFHNKMPSDVGLESDTMFPYEPYNPLVFSGFSSISASLRRAFGNNVPLLSSQQSFSTHTGINGEVTNSQIQSTNELSTHSVANGHSDILTKDDFGEEKALDNDGISVHNEPQLETQSSVGENEDQSQFKDGSSKMLNADSILILMSRRNSSTGSICEQSHFSHIKFYRNFDVPLGIFLRDNLLNQRLLCSLCGGSPEAHIYYYAHHNKQLTIQVKHFPMEKHLPGETDGKIWMWSRCGNCKPQSGNTQSTKRVLISADARGLSFGKFLELSLSNHLSSCSFSICGHPLHTDCLYFFGLGPMIAMLRYSPVTTYTVSLPPEKLKFDSQISDERLRKAFDSEDLLNASKNWDSGDPIYKVVSLNRVQWDMLVASCVWDRRLHSLLSSEITTAAPTCHKQMQDQSNVEEDGYVGEHLDNAVECETNGTNLDKFEDTAVQTTDVIEVPIEGDTQGSSGQYSLLTTYLDEEGMHKLSGGMSNSDGSNNQYLSNPDPSSDSQSADAQNIALTAYDLPAEANDSNLSERKFSVLKVSSAENSEWIWSPFQDIRNEYLKDLQKGYSMKFEPVNTYPQGSRIQNVINDEGSRLHIPLGIDNSIVSDHEDELSSIVACALALLKDPSVLVDRNEDPTRERGLDSKLYEKSYSVSRPSSLNSSYWSSFGSFTSDGIYSSASGLDDSSLSSTNGLYLSDSLIYFEDMHLEVSMGLGKLPGKGKYSVVSLYASQFQDLRRRCCPSELDYIASISRCRNWDAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFLEFAPHYFKYMKDCFEQGNQTCLAKILGIHQVTVRQKGGKETKHDLMVMENLMFGRNISRQYDLKGALHARLNSSPDGSGDVLLDQNFVNDMNNSPFYVGRQAKRNLQRAVWNDTTFLNSINVMDYSLLVGVDTQNGELVCGIIDYVRQYTWDKQLENWVKSSLVPRNQLPTVISPKEYKKRFRKFITTHFVSVPDHWCSQILSNSGEEDDSFHGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4S8</accession>
    <name>A0AAF1B4S8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0623402</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4S8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF149">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="111"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH03997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="28598" checksum="D31A72396B94A6BE" modified="2024-05-29" version="1">MNSSKSSSTWGSSSFTIGEKICAVFIPFVALCEALVCNLAACFHLKPSSSPAQPILYTFNDIVRLSQETPFTVNEVEALRELFKKLSSSIIDDGLIHKEELQLALFNSSAGKNIFLDKVFDLFDEKQNGVIEFEEFVHVLGVFHPAASITEKIEFAFRLYDLRQTGFIEREEVMQMVVAILKETNASLSDEILEAIIDKERDITYADKDGKISKEEWKSFAVRHPTLLKNMTLPHLKDITTAFPSFIFNTGVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4T0</accession>
    <name>A0AAF1B4T0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0624054</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4T0"/>
    <dbReference type="KEGG" id="dcr:108227605"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH04643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="28634" checksum="54497FB2B1892059" modified="2024-05-29" version="1">MYLGGEMQKAKAITLYQIAQIGHLGLLLLWHLVHLIVNIWYFALGLGQTLESYLISSGLLKRYNSLDSNRVRYLAIVVDSEEACQTSKVLELLQHLENIGIKHVCLYDPEGLLKKSKEAIFEKLTRVKLYEEATKSDSLVRKHITLELASFSDGKKAVAEAANYLYKKNYIGGEQKEFICSESSIDEALKAIGCAHADPDLLLVYGPARCHLGFPAWRLRYTEIVHMGPLKSMKLGSLIKAIYNFTKVHQNYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5Z9</accession>
    <name>A0AAF1B5Z9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0624656</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Leucine-rich repeat protein that likely mediates protein interactions, possibly in the context of signal transduction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5Z9"/>
    <dbReference type="KEGG" id="dcr:108226139"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000405">
        <property type="entry name" value="Plant intracellular Ras-group-related LRR protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH05242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="501" mass="56685" checksum="87224B3B164BB082" modified="2024-05-29" version="1">MDPSPRSLPILSYVMKKFPSFKRPAPSDFDVEQPLPMSPSSLPSGPYFELKERMPHLTNPKLISQMRSAVADVSQTRSILKKLGPRPDHEAVDIAKARLAEIENELEEVELAEANADNLKKKEAEREKQMYKTVISLDEMHESYGKLLTDAETRLEKIYEAAAAGYNGEDDVAVVEEVNEEVVRILQEANQREVQRIDLAKKHLRFLPEAFGKIRPLVVLDLSSNQFQMIPDSIAGLENLEDLNLSSNILTSLPESIGLLLKLKTLDVSNNKLVALPDSICHCKSLVELNASFNKLAYLPTKIGFELVSLRRLSIQLNKIRSLPTSIGEMSSLIFLDVHFNELQGLPRSIGNLTHLEFLNLSSNFSDLTELPDTFGELTNLQELDLSNNQIRELPLTFRHLNNLRKFNIEENPLVIPPKETVKNGVEAVRAYLTQRYHQLLLEEEEKERKRIEAQDESPKGWFTRSTSWLKDSVTTVSGNVSEIMGVEGTRNQDPLLNQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6Y0</accession>
    <name>A0AAF1B6Y0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGGY carbohydrate kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0626337</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6Y0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07782">
        <property type="entry name" value="ASKHA_NBD_FGGY_D-RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000101">
        <property type="entry name" value="FGGY carbohydrate kinase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006003">
        <property type="entry name" value="FGGY_RbtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01315">
        <property type="entry name" value="5C_CHO_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="6">
        <location>
            <begin position="54"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="7">
        <location>
            <begin position="332"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH06908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="600" mass="65047" checksum="33A37BF09DD1AEDC" modified="2024-05-29" version="1">MTEPQRISTTDTTPFLSPQDRDGYVHQILSPIPSPTRPIPSFSMSSSSPCRSVFLGIDVGTGSARAGLFDESGKLLGSASSPIQIWKQGDCIEQSSTDIWLAVCSAVKSARSLAKVNANEVKGLGFAATCSLVAVDSDGEPVTVSWSGDARRNVIVWMDHRAVNQAERINSSNSPLLWVKENLQESWSMVFRWMDLSDWLSYRATGDDTRSLCTTVCKWTYLGHAHMEVNKKDLRNMEACGWDDEFWEEIGLGDLMEAHHAKIGRSVAFPGHALGSGLTVDAANELDLLAGTPVGTSLIDAHAGGVGVMESVPEADSKSEDIDADTICQRMVLVCGTSTCHMAVSRSKLFIAGVWGPYWSAMVPEYWLSEGGQSATGALLDHIIENHVASPHLANRAASQSISPFQLLNKILESLMHDRGSPFLAALTSDLHVLPDFHGNRSPVADPKAKGMICGLTLDSSEKHLALLYLATVQGIAYGTRHIVEHCNAQGHKINVLLACGGLSKNTLYIQEHADIIGYPIILPRESESVLLGAAILGSVAAKKYSSLSEAMKALNAAGQVIHPSTDPRVKKYHDGKYRIYRELYQQQLSHRAIMAEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B921</accession>
    <name>A0AAF1B921_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0728674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
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    </evidence>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BE78"/>
    <dbReference type="KEGG" id="dcr:108200779"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF98">
        <property type="entry name" value="POTASSIUM TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="11">
        <location>
            <begin position="23"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="12">
        <location>
            <begin position="533"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH16519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="779" mass="87352" checksum="13F728415BCAAC72" modified="2024-05-29" version="1">MDLETGTYKNLVKRESWRAVLTLAYQSLGIVYGDLSTSPLYVFKSTFAEDIDHSETNEEIYGVLSFVFWTITLVPLIKYVFIVLKADDNGEGGTFALYSLLCRHARVSSLPNNQLADEELSSYKKEISVDPDPSKFGSMLKSILEKHRVLQRILLILALIGACMVIGDGILTPAISVFSAVSGVEQLAMPKEHHKYVEVPIACIILIGLFALQHYGTHRVGFLFAPVVILWLFTISSIGLYNIFHWNPHVYKALSPYYMYNFLKKTQRRGWMSLGGILLCITGSEAMFADLGHFSQLSIKIAFTTFVYPSLILAYMGQAAYLSRHHMSANDYDVGFYVSVPDRIKLPVVVIAVLAAVVGSQAIITGTFSIINQCSSLGCFSRVKVVHTSTSIHGQIYIPEINWILMILCLAVTIGFRDTRRLGNASGLAVITVMLVTTCLMSVVMVLCWHQSIFRAICFVCFFGTIEALYFAASLIKFLEGAWVPIALALIFMIVMYVWHYGTLKKYEFDLQNKVSVDWLLSLGPSLGIVRVPGIGLIHTELVSGIPAIFSHFVTNLPAFHEVLVFLCVKSVPVPHVEHGERFLLGHIGPRECRVYRCIVRYGYRDVHKDDMEFEKDLLCSIAEFIRTQKSDTNGLDKHSAHDTEKMMVVRTPSTSTEGIHMWEDNLDDSDKIITSEEKSIDLPEVTKARKRVRFILPENPKIDREAEAELHDLMEARESGVAYILGHSYVKAKQGSSLMKKLAINYGYEFLRRNSRASTHALSVPHASTLEVGMGYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X2I3</accession>
    <name>A0A161X2I3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0206804</name>
        <name evidence="13" type="ORF">DCAR_0206806</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X2I3"/>
    <dbReference type="Gramene" id="KZN05213">
        <property type="protein sequence ID" value="KZN05213"/>
        <property type="gene ID" value="DCAR_006050"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN05215">
        <property type="protein sequence ID" value="KZN05215"/>
        <property type="gene ID" value="DCAR_006052"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208916"/>
    <dbReference type="KEGG" id="dcr:108208917"/>
    <dbReference type="OMA" id="KEVTAPC"/>
    <dbReference type="OrthoDB" id="5570111at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG87575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG87577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="449" mass="49337" checksum="B0BDB4F08D595247" modified="2016-07-06" version="1">MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETNKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIAFVPISGFEGDNMIERSTNLDWYKGPTLLDALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGTLKPGMVVTFGPSGLTTEVKSVEMHHESLQEALPGDNVGFNVKNVSVKDLKRGYVASNSKDDPAKGAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELLTKIDRRSGKELEKEPKFLKNGDAGMVKMLPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVIKAVDKKEPTGAKVTKAAVKKGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X459</accession>
    <name>A0A161X459_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207399</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X459"/>
    <dbReference type="Gramene" id="KZN05735">
        <property type="protein sequence ID" value="KZN05735"/>
        <property type="gene ID" value="DCAR_006572"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206511"/>
    <dbReference type="OrthoDB" id="2020802at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000002">
        <property type="entry name" value="B3 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF123">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR NGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="444" mass="49975" checksum="F33A7155E505383C" modified="2016-07-06" version="1">MMMIDNNGGENDVDEVIIEGRRRDGHVIVEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDSSTNEKGLLLNFEDRSGKQWRFRYSYWNSSQSYVMTKGWSRFVKEKKLDAGDVVSFQRGMGEIGRDRLFIDWRRRPDAPSSSASSHYHHLLGTGLVPSASSLALQPQYYSSFQRASNAPWSPSLFLQQQQQQPLSSTPQYYPNYPHNYRDQYSSHLLLPPPPNVTGHHNPHSHFYHPTNYMSATRSTAHHHPLVSPAGPCSGSVIYYNRSAPFSQEQEQQHEEDVEMAQAQRAGWGEFEPNVVFESVPVVHGKTAPKRLRLFGVNMECPINTSGSSNSDDNIIVECDNNMTILSSSSTASAMNIPNIRTSQGPYPYNFCSSAPPQPLPPLMSNPYDTSSSNIPYVQLSPQTATTSTTSTLHDLMNKGKMSMSFDRNEGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y587</accession>
    <name>A0A161Y587_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="12">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0727831</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="12"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y587"/>
    <dbReference type="Gramene" id="KZM87399">
        <property type="protein sequence ID" value="KZM87399"/>
        <property type="gene ID" value="DCAR_024533"/>
    </dbReference>
    <dbReference type="OMA" id="WFRGVQY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000005">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH08392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="471" mass="52113" checksum="AC8388FEE5E96485" modified="2016-07-06" version="1">MDPVNAWGNTPLITIDPEMHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDQIENLCRSRALQAYRLDSAKWGVNVQPYSGSPANFAAYTALLNPHDRIMGLDLPSGGHLTHGYYTSNGKKISATSIYFESLPYKVDPKTGYIDYDKLEEKALDFRPKMLICGGSSYPRDWDYKRFREVADKCGALLLCDMAHISGLVAAQEAADPFEYCDIVTTTTHKSLRGPRSGMIFYRKGPKPAKKGQPEGAVYDFEDRINFAVFPSLQGGPHNHQIGALAVGLKQAASPAFKAYAKQVRANAVAVGKYLMSKDYTIVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCSITLNKNAVFGDNSALSPGGVRVGTPAMTSRGLLEKDFEQIAEFLHRAVTVTLKIQKEHGKLLKDFNKGLDNNKEIEQLKTDVEKFASSFDMPGFSVADMKYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YKI1</accession>
    <name>A0A161YKI1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YKI1"/>
    <dbReference type="Gramene" id="KZM93919">
        <property type="protein sequence ID" value="KZM93919"/>
        <property type="gene ID" value="DCAR_017164"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223839"/>
    <dbReference type="OMA" id="DYIIMIT"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001473">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF53">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH00262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="621" mass="69801" checksum="D4ECD64E7342BEBF" modified="2016-07-06" version="1">MELPVKHPVSNGRGVLPYTIEAKKLSYSFHSLYADFSFSSCWRSQNTCPKYILKDVYCEARPGEITAIAGPSGAGKTSLLEILGGKIAPSKISGKVLINGVPADAKSFRKLSGFVTQDDALFPLLTVEETLMYSALLRLPGGRKEAGTRVTVLMRELGLDHVAGTRIGEGSNNGISGGERRRVSIGVELVHNPAVVLIDEPTSGLDSASALHVVSLLKAMVVQQGKTILLTIHQPGFWILELLDRLLLLSNGFVLHNGSLKLLEDRLKFVGHFIPPHINVLEFAIDVTSSLVIQTSDDFSNQILLKKQEDLQESEHDYDMSSLFHANRAEEHKSVASPNSHLEEIQILGERFFKNIFRTKQLFFTRIIQALFTGIILGTIFINVANDQGNLALQARIGFFAFSLTFLLSSTTEGLPIFLQERRIFMRETSRGAYRVSSYVISNTLIFIPFLLMIALLYTTPVYWLVGLRRNADGFMYFSLVVWMVILMSNSFTACFSALVPNFIMGTSVISGLMGSFFLFSGYFISKENIPRYWIFMHYLSLFKYPYECFMINQYGGEGGRRKCIQFEGGKCKMYGEGFLKQQGLRESQKWSNLGVMLGFIIGYRVLCFLILWFRCYRTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A2E4</accession>
    <name>A0A162A2E4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferulate 5-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_017335</name>
        <name evidence="13" type="ORF">DCAR_0519819</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A2E4"/>
    <dbReference type="STRING" id="79200.A0A162A2E4"/>
    <dbReference type="Gramene" id="KZM94090">
        <property type="protein sequence ID" value="KZM94090"/>
        <property type="gene ID" value="DCAR_017335"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224075"/>
    <dbReference type="OMA" id="RQEMYIG"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000054">
        <property type="entry name" value="Cytochrome P450 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053062">
        <property type="entry name" value="CYP450_84A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945:SF5">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM94090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH00456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="57497" checksum="F90CE9E7CD5DFF02" modified="2016-07-06" version="1">METNATTAMTILFFILPLLSFFILSSFKRKRFPPGPKGWPIIGNLLMMDTLTHRGLAKLAARYGGLVHLRMGFLHMFTVSTPDMAREVLQIQDNIFANRPATMNISYLTYDRADMAFANYGPFWRQMRKISVMKLFSRKRAESWDSVREEVDDMVKIVVSNTGCSVNIGELVFGLTRNIIYRAAFGSLSHEGQDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGRLVKARASLDGFIDSIIDEHIEKKKSCKIDEGNSDMVYELLDFYSEDKATVSVSEDLNTSLKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKVQQELNNVVGLHRRVEESDFDKLTFLKCCIKETLRLHPPIPLLLHETAQDAEIAGYHIPARSRVIINSWAVNRDPNSWADPDTFKPSRFLKEGMPDFKGSNFEFIPFGSGRRSCPGMQLGLYALEIAVAHLLHCFSWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AB10</accession>
    <name>A0A162AB10_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_014016</name>
        <name evidence="10" type="ORF">DCAR_0416942</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AB10"/>
    <dbReference type="Gramene" id="KZM98622">
        <property type="protein sequence ID" value="KZM98622"/>
        <property type="gene ID" value="DCAR_014016"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217747"/>
    <dbReference type="OMA" id="FLWNLEA"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="UniPathway" id="UPA00213"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016138">
        <property type="term" value="P:glycoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000143">
        <property type="entry name" value="UDP-glycosyltransferase 89B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF8">
        <property type="entry name" value="FLAVONOL 3-O-GLUCOSYLTRANSFERASE UGT89B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM98622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="473" mass="52725" checksum="C2EC47BD680A8E31" modified="2016-07-06" version="1">MAISEAGAHILIIPYPAQGHMIPLLDLTRQLALRNLTITILVTPKNLHYLNQLLIKHPSIQPLVLPFPAMAALPPAGVENVRDLPPGSFRLFTMVLADLYDPIVKWFQSHSSPPVAVVSDIFLGWTNRLANELKIPRYVFSPSGALALSVVYNLWNNMPRRNDPTDKNEVIEFCDIPNCPSFPWWKISPVFRSYVAGDPQYEVFKDSFREDIASWGLVINSFTELERIYLDYLKEFLGHDRVWAVGPLLPPREEQVKRGGFSGNLANEIELWLDQFEAGTVVYVCFGSQVVLSNKQMEMLALGLEKSAVRFLWSYKAPTEEDVLENYGVMPLRFKDGVGERRLIVKGWVPQVSILSHQAIAAFLTHCGWNSVLESIAAGVPMLTWPMGADQFANADLVDELKLGTRLCEGEEMVPDPDELARVVAQSVTDSRGGKGVRAEELSKASVDSIAQGGSSCKALNCLVDFLSRRETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AIY7</accession>
    <name>A0A162AIY7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_010355</name>
        <name evidence="2" type="ORF">DCAR_0311787</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AIY7"/>
    <dbReference type="STRING" id="79200.A0A162AIY7"/>
    <dbReference type="Gramene" id="KZN01601">
        <property type="protein sequence ID" value="KZN01601"/>
        <property type="gene ID" value="DCAR_010355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN01601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="163" mass="18532" checksum="B06731631F24273A" modified="2016-07-06" version="1">MLVIPFVQHLVWYYRLEAEKLESIVQRLDYQVAYKWLCELRTCIFAVSLRSSSSLESKGSSIEMLCGNAIWPKEGGGFVNGNGGKTPDPSYLVSKPWRPLTRKLKIPSAVISPYRTPQKNGRNELLKRVVAGGGAFKRPGWHKSSSSRSKPKRHSFDYVLYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B0B3</accession>
    <name>A0A162B0B3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_000939</name>
        <name evidence="12" type="ORF">DCAR_0100896</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Preautophagosomal structure membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B0B3"/>
    <dbReference type="STRING" id="79200.A0A162B0B3"/>
    <dbReference type="Gramene" id="KZN08393">
        <property type="protein sequence ID" value="KZN08393"/>
        <property type="gene ID" value="DCAR_000939"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205238"/>
    <dbReference type="OMA" id="IPTGECV"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN08393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG81745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="908" mass="104349" checksum="D09434BF6747BC33" modified="2016-07-06" version="1">MMLSRQNGANALDILRRKLRGESSLRTSLLDDSHEIELSDYHRAPSSGSESPSGLLNGDSLSVEPIADLDLFFERLYSYYCEKGLLCIVIKWIVELLSLGFTICFSCFFLLYVDWNGLRNAKCGMVAVESGIKPCDLVKEAFHEHPLIPFTLSKAIIVGYLGIFSIYWIICFLRFFAQLKEILKIRQFYYNSLCITDNEMQTMQWASVLEKVVQLQNSRQLCVVKDLSAHDIVMRLMRKDNYLIGMLNKGVLSFPVSKWVPGAGPIVKSGPMGTQQRIILTKILEWTLKWCILQSMFDRNFCVRRDFISDPKTLKKRLFIVGVTVLVLSPFIVIFMLVYLFLKHAEQFYNHPSTASSRRWSNLSKWILREFNEVDHLFKHRINGSVVHASEYLKQFPSPVLSIIAKFISFVSGGFAAILIIIAFLEESLLEGHIFGRNLFWYAAVFGTITAISRAAVSDDHLVLDPHGAMSLVVQHTHYMPKRWRGKENSNDVRVEFETLFQYTGMMLLEEMASIFLTPYLLIFIVPERVDDILQFIDDFTVDVEGVGHVCSFSAFDFQNHGNIRYASPYNSARVQRSSQGKLEKSFLSFQSSYPSWEPNSQGKQLISTLKAFREQKLRVQERIHPYLPPRMQQWSPETRVHGDLNNVFTREAFHNIQERGYQLGSVWLFNSELKNYLNILEWYYTSGPHLANEAERGSPSRSSNVAESTRFFWMPPDVRHNEATDEENWGQFFDNDRSQSPLEASTAAPLFHGSVLHHDDSVNTAEHPSSKSHWWARSDAQGNNRHTSFLEPPAFYRNTDTFHNNAQTSFLELPTFHRNTNAYYDNNPQTSFLEPPTFDSNPDTFDDNMFGSSIVQEDQDRSGSMRSSNRSHSIYIGDIDEGQFNLPFDDIYSRHSTSPELDPSNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B1E8</accession>
    <name>A0A162B1E8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_001399</name>
        <name evidence="3" type="ORF">DCAR_0101333</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B1E8"/>
    <dbReference type="Gramene" id="KZN08743">
        <property type="protein sequence ID" value="KZN08743"/>
        <property type="gene ID" value="DCAR_001399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG82171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="115" mass="12874" checksum="29603D3E13507644" modified="2016-07-06" version="1">MYKKISESRSKGGNKMALVAKEELKDMDMAAAAEQLMQLSGDEENDNTVTCSDTGNADERKNYSNAVVAQSHVGDDGRSSRNHQVLGGLRRLVIKKRRKYRSILHLYMSTKPVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VBC2</accession>
    <name>A0A164VBC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_022551</name>
        <name evidence="4" type="ORF">DCAR_0623615</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VBC2"/>
    <dbReference type="STRING" id="79200.A0A164VBC2"/>
    <dbReference type="Gramene" id="KZM90084">
        <property type="protein sequence ID" value="KZM90084"/>
        <property type="gene ID" value="DCAR_022551"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227413"/>
    <dbReference type="OMA" id="MMRGMSA"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031977">
        <property type="term" value="C:thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046322">
        <property type="term" value="P:negative regulation of fatty acid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010117">
        <property type="term" value="P:photoprotection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009644">
        <property type="term" value="P:response to high light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901562">
        <property type="term" value="P:response to paraquat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010431">
        <property type="term" value="P:seed maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF32">
        <property type="entry name" value="CHLOROPLASTIC LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="129"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM90084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH04206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="345" mass="38464" checksum="6A2DDE4CB197F6C8" modified="2016-07-06" version="1">MVYHNIVVSAAVAHFSPVVLLRTHSSPFHFNIPRSEAGRIKFTCSVEEQHVWNNTVGVRKAISGLAASFVLLSQTSQVLGADLPHPQNVYGLANASDNLPTLPLDIMPEEKLGMMMMTKGMTAKNFDPVRYSGRWFEVASLKRGFAGAGQEDCHCTQGVYTFNEKKAAIQVDTFCVHGGPDGYITGIRGNVQCISDEDLEMSETDLEKLEMVKGKCYLRFPTLPFIPKEPYDVIATDYDNYALVSGAKDKSFIQIYSRTPNPGPEFIEKYKLYLADFGYDPNKIKDTPQDCDVSDSRLAAMMSMNGNQQALTNQFPDLELKSAVAFDPFTSVFDTLKKLIQLYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W9V2</accession>
    <name>A0A164W9V2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0625181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W9V2"/>
    <dbReference type="Gramene" id="KZM91487">
        <property type="protein sequence ID" value="KZM91487"/>
        <property type="gene ID" value="DCAR_021148"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224564"/>
    <dbReference type="OMA" id="QESKMLE"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000041">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF119">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A 59 KDA REGULATORY SUBUNIT B' ZETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH05760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="537" mass="60607" checksum="F2483FCFAF8E2164" modified="2016-07-06" version="1">MIKQLISKLPKKTSNKSSNNDPETAFSSMNTINSAGADNSSKGGSLSGKGSIISNGNHGPQSSSVTLKSNQGMQSGSVAARAGQGISYAAYEALPGFREVPSSEKHSLFIKKLNMCSVLFDFNDASKNLKEKDIKRQTLLELVDYISSVTSKFNEVTMQEITKMIAANLFRALPSNHDDRLPDVYDPEDDEPTMEPSWPHLQVVYEFLLRFVASSGTDAKLGKRYIDHSFVLRLLDLFDTDDQREREYLKTILHRIYGKFMVHRPFIRKAINNIFYTFIFETGRHNGIAELLEILGSIINGFALPLKEEHKLFLVRVLIPLHKPGSLSMYHQQLSYCITQFVEKDAKLADTVIRGLLNCWPITNSSKEVMFLGELEEVLEATQTAEFQKCMVLLFRQIGRCLNSSHFQVAERALFLWNNDHIRSMITQNRKVILPIIFPALENNTRIHWNQAVHSLTLNVRKIFIDADQALFEECLAKFQEDETKEEERLEMRELIWKRLEDIAASKAASNGAVLVSNFSSSLAIATSANSPKITSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YP97</accession>
    <name>A0A164YP97_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0520662</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YP97"/>
    <dbReference type="Gramene" id="KZM94805">
        <property type="protein sequence ID" value="KZM94805"/>
        <property type="gene ID" value="DCAR_018047"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223464"/>
    <dbReference type="OMA" id="CKSWAGI"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF71">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043724814" evidence="13">
        <location>
            <begin position="22"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH01280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="639" mass="70118" checksum="DB729750BC3152EA" modified="2016-07-06" version="1">MKIHFLGVVTTLLFVVHNLRATDADLSSDRQALLDFAAAVPHQRKLNWNVSVPVCTYWVGIQCNKNGTRVIAVHLPGVGLYGSIPVKSIGKLDALQVLSLRSNYLIGDLPSDIPSIPSLQALYLQHNNFTGHIPLSLSPHLTAIDLSFNSFSGIIPPTLENLTKLTLLDLQYNLFSGAIPSLNNTRLRLLNLSHNQLNGTIPNVLEKFPTSSFIGNSLLCGPPLTNCSALSPSPSPSADSSPAVAKHHGSRHFGKFGLGSIVAIVIGAVLILFLVFVIFLCCFKKKKSEVSIVSKPKVTNNGKNEKSEEYFGSGIQASEKNKLFFFEGSTYSFDLEDLLRASAEVLGKGTYGTAYKAVMDEGTTVVVKRLREVGVAKKEFEQHMEILGKISRHPNIVPLCAYYYSKDEKLLVYEYMPASSLSVCLHGNRGTGRTTLDWESRLKIALGAARGIAHIHSEGSVRLTHGNIKSSNVLLNRDLDGCVSDAGLAPLMNSVPAKFRGPGYRAPEVIESRKVTQKSDVYSFGVLLLEMLTGKSPIQIAGHGNDEIVDLPRWVRSVVREEWTAEVFDVELMKYQNIEEELVQMLQIALACVAKTPDMRLSMDEVVKMIEEIRQCDSENRASSDDNNSKNSNVQTPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z6N7</accession>
    <name>A0A164Z6N7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_018666</name>
        <name evidence="14" type="ORF">DCAR_0521360</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the patellin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z6N7"/>
    <dbReference type="Gramene" id="KZM95424">
        <property type="protein sequence ID" value="KZM95424"/>
        <property type="gene ID" value="DCAR_018666"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219868"/>
    <dbReference type="OMA" id="HCYDKVG"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.20:FF:000008">
        <property type="entry name" value="SEC14 cytosolic factor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056794">
        <property type="entry name" value="PATL1-6_C_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF4">
        <property type="entry name" value="PATELLIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25099">
        <property type="entry name" value="GOLD_PATL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="11">
        <location>
            <begin position="131"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="281"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM95424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH01973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47536" checksum="AD8135A91F1ABDEB" modified="2016-07-06" version="1">MSQMESSSSPFLPTLQTPLSEASPKPFKKSLVASFMEATTLRSPSFKEDTYFLTHLKPSEKKSLQELKEKLKASHEAVSMWGISLLSGDEKADVILLKFLRARDFRVQDSLQMLLKCLEWRKDFEADGIVEQDLGFKELEGVVAYMNGYDKEGHPVCYNAYGVFKDKEMYEKTFGDDFKLEKFLRWRVQVLERGIKLLHFKPGGINSIIQVTDLKDMPKRELRVASNHILSLFQDNYPEMVARKIFINVPWYFSVLYSVFSPFLTQRTKSKFVISKEGNVAETLYKFIRPENIPVQYGGLSRLGGSENGPSKPASEFTVKGGEKVNIQIEGIEAGATITWDIVVGGWELEYCAEFVPIAEGGYTIAVEKPRKIAAAEEAVHNLYTAKEAGKMVLSVDNTSSRKKKVAAYRYIVRKSTSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZM63</accession>
    <name>A0A164ZM63_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_019358</name>
        <name evidence="3" type="ORF">DCAR_0522154</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZM63"/>
    <dbReference type="Gramene" id="KZM96116">
        <property type="protein sequence ID" value="KZM96116"/>
        <property type="gene ID" value="DCAR_019358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM96116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="11177" checksum="71B3CEB67B9B3B6A" modified="2016-07-06" version="1">MEGSFISLLEEGSIPEIEDMMFSQPTQSQSQPQTQNQPQLQKEALKLKKGKRSKNFLIQEDMLLLSAWLNNQTHTNYWARIWKYYKENKKKARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165AAV9</accession>
    <name>A0A165AAV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_015422</name>
        <name evidence="9" type="ORF">DCAR_0415340</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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                <person name="Ellison S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG97595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="31523" checksum="E271FBFD7D4EDE55" modified="2016-07-06" version="1">MELWKRARTLAEETAKRSHDFTVEAARKSQAFTAEAARRSQDFSIGSSKLADIVSEASRKADLIRVEAIKRADQIKSKIPNASALSKIGVSSHEEEEEDLERFGVTEELTDFVRNISVSTFKEFPLQDDTEMSDIPTVSNIRQDLTKWQEVHAKLVLSTVKEISKLRYELCPRVMKERKFWRIYFILVNSHVAPYEKRYNEDARRRSAEEVKNDMKSSEVGTLSKSETGKTVQKTKSVSSAAAEQDLDVFLLGDLGDSDDAPDGKDEGFDDDDDFDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z0Y8</accession>
    <name>A0A165Z0Y8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_013154</name>
        <name evidence="4" type="ORF">DCAR_0417926</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z0Y8"/>
    <dbReference type="Gramene" id="KZM99484">
        <property type="protein sequence ID" value="KZM99484"/>
        <property type="gene ID" value="DCAR_013154"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217636"/>
    <dbReference type="OMA" id="HQFANSY"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25156">
        <property type="entry name" value="PNGase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007869696" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="57"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM99484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG98582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="612" mass="68028" checksum="0A0F58C8BB699F68" modified="2016-07-06" version="1">MAFSLLSVLLFSLCFFHSTAKLPKSSLSLFRSQFSETPNDISPTTFFEVTKPIQVPNTKPCSQLILDHDFGYTYNKPPVLADYKPSFLNCPSQDFSRIVLEWKASCKGRQFDRIFGVWLGGVELLRSCTAEPTSNGIVWTVQKDVTRYYSLLMTNQTLAVYLGNVIDSTFTGVYHVKLSLHFYPVEEHILNWGNLGHGYGSNADLILPISRTLPLNDGLWFEVVNSTDVKSKVFQIPENAYKAVLEVYVSPHENDESWYTNLPSEYIARNNITDLPGNGPFREVVVSIDGKVVGAVWPFSVIYTGGVNPLLWRPISAIGSFDLPSYDIDITPILGTLLDGETHELGLSVTNALNVWYIDANLHIWLDGKSEKTEGKVLRSSTSPCHVTYVSNFTGMNGTFSTKVSRLIDSIGWVKSSYGEITTQATQEFNFSNFMEMGSNGNLQIVHQRIDFIDTTSANSGTSVDSVTSLKRFPLYLYMENADQEGGAYTSVSNLSLGFNEKIIKKSEFGSSVSSLENLQGGQASMLVKGNLVVSGTGRTQQVYSFDSSNSCYLRNISSSNYTIIYDQESNKCNKRTATHWGSGLHKWGRQPIRSAYLVSDSVKGKRGFNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z4Q4</accession>
    <name>A0A165Z4Q4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0418067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z4Q4"/>
    <dbReference type="Gramene" id="KZM99607">
        <property type="protein sequence ID" value="KZM99607"/>
        <property type="gene ID" value="DCAR_013031"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219239"/>
    <dbReference type="OMA" id="MEHGSEP"/>
    <dbReference type="OrthoDB" id="1726347at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008889">
        <property type="entry name" value="VQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039609">
        <property type="entry name" value="VQ_15/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179:SF29">
        <property type="entry name" value="OS06G0666400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33179">
        <property type="entry name" value="VQ MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05678">
        <property type="entry name" value="VQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG98722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="223" mass="23978" checksum="5FEE90CE4F2AE0CD" modified="2016-07-06" version="1">MSQTMSSPGNNWDFYQHNAPAKPVGLFSSNQVSDATTVTATHIGSASGGGGLSPEGRVAKPVRKRSRASRRTPTTLLNTDTANFRAMVQQFTGGSLAPFASGASSTSSYPIATNFSLGIGTGQQYGNPNAGMIPRPGYNILQVQQQLHQQQQQQQHQFMFSKNDNPSDDDHHAYLRGLERSSIPHMESSGGVDFRLDGVSSSVPPIIRSSSSSQNLSEDNYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZJD0</accession>
    <name>A0A165ZJD0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0310012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZJD0"/>
    <dbReference type="Gramene" id="KZN00104">
        <property type="protein sequence ID" value="KZN00104"/>
        <property type="gene ID" value="DCAR_008858"/>
    </dbReference>
    <dbReference type="OMA" id="LPETCKG"/>
    <dbReference type="OrthoDB" id="1743799at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595:SF3">
        <property type="entry name" value="PAS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33595">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="31212" checksum="589B19F697CA59A1" modified="2016-07-06" version="1">MMQTIDSNKTAQILARYRPIAPKPPDHETNISTNSSPNSDSSCMPLNIRDSAYLRTVWPQLQSRSTRGRKRGRTSVPPPLPCKRSKPAHLPSPIPNQIPNQMIIPVEKNLVKHLELHQSPNLFTPKPIRLVSSSILIQPNSTLLSPALTNPGHSTADEIEKGIESQTLPVLISDSKNQVRLANSAYKRLIGQPECNWLESITACNRICGEVTLKIPESVVSELASSSKGVNFCLVVIEWGNSEYKSSITTSGEAVRLRCESRDYVFAWRFHTQGALVPAANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZQC2</accession>
    <name>A0A165ZQC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0310242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YTHDF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZQC2"/>
    <dbReference type="SMR" id="A0A165ZQC2"/>
    <dbReference type="Gramene" id="KZN00298">
        <property type="protein sequence ID" value="KZN00298"/>
        <property type="gene ID" value="DCAR_009052"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215163"/>
    <dbReference type="OMA" id="MAYGQFS"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF77">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="600" mass="65934" checksum="16959CFA5DFCD5AD" modified="2016-07-06" version="1">MEESGQQGPDRVVPVSNSTSPVAVESHFSPDNRIEQILQPKEDKVVSASSLNTTIAGPPSIITGPPEILASSGALATSYQPNAYAPLEQSFYYGGYENNAGNWGEYPNYVNANNLQIIPPAMFNDNSSLFYPSGYSFDTQMAYGQFPPLASPMSPFLVDGQLYSPHQIPMSPNFYPQSISPGLPHVGSSHSMSQTEMATPVSTGQDSVNDNMLLGPGSGYYLHFGSFPASSAPANSSLGLYKYPGEFGSGENPLIRSNSNDTGNILSPLTPGAVYTQPVGILGSYDHSLAQASQQQIPFTRRYHNSTSSRMSNYGNWDASRSNRFTPDKGGRRDRDSISISADSHGNANDRNRGPRALKPKIKTSTEEKAAPGVAKAGASTSGVNLDLYNQPDFVTDYERAKFFVIKSFSEDNVHKSIKYCVWASTPLGNRKLDAAFQEAKDSNSIYPVFLFFSVNASGQFCGVAEMTGPVDFENDADYWQQDRWSGQFPVKWHIIKDIPNGRFRHILLDNNENKPVTHSRDSQEVKLEQGLAMLKIFKEHEADTSMLDDFVYYDEKERSLQEKRAKQSSPGKIPDASITQLSENLAGTLHLKVIEDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A5M9</accession>
    <name>A0A166A5M9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the plant dehydrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A5M9"/>
    <dbReference type="Gramene" id="KZN00768">
        <property type="protein sequence ID" value="KZN00768"/>
        <property type="gene ID" value="DCAR_009522"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215439"/>
    <dbReference type="OMA" id="HHEPESY"/>
    <dbReference type="OrthoDB" id="1934367at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000167">
        <property type="entry name" value="Dehydrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030513">
        <property type="entry name" value="Dehydrin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346:SF2">
        <property type="entry name" value="DEHYDRIN ERD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346">
        <property type="entry name" value="DEHYDRIN XERO 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00257">
        <property type="entry name" value="Dehydrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00315">
        <property type="entry name" value="DEHYDRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00823">
        <property type="entry name" value="DEHYDRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="213" mass="23797" checksum="1BB884868FBE0BC8" modified="2016-07-06" version="1">MASDDSVPQHSVEKTTEYESSDRGLFDFMKKEEKDETKVIATEFEEKVQVSEPEPKYEDCKVVEEEEEKAAKPSLLEKLHRSGSSSSSSSSDEEVEEGGEKKKKKKEKKGLKEKIEEKIHHKEEDTSVPVEVVTEPEEKKGFMEKIKEKLPGGGKKVEEETVAPPPPPAAAPVDCAVEGEPAKKGILEKIKEKIPGYHPKTSTEEEKKDKDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ADY1</accession>
    <name>A0A166ADY1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0311129</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a key role in the control of the eukaryotic cell cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ADY1"/>
    <dbReference type="Gramene" id="KZN01104">
        <property type="protein sequence ID" value="KZN01104"/>
        <property type="gene ID" value="DCAR_009858"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211615"/>
    <dbReference type="OMA" id="CFCHQRN"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000281">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000231">
        <property type="entry name" value="Cyclin-dependent kinase B2,2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF178">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE B2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="310" mass="35274" checksum="5E5205B0306CF8F6" modified="2016-07-06" version="1">MENSAVSAMDAFEKLEKVGEGTYGKVYRAREKSTGKIVALKKTKLHEDSEGVPSTTLREISLLRMLSRDPHIVKLMDVKQGINKAGNTVLYLVFEYMDTDLKKFIRSFRQTGENIPSPTVKSLMYQLCKGVAFCHGHGVLHRDLKPHNLLMDPKTLALKIADLGLARAFTLPIKEYTHEILTLWYRAPEVLMGATHYSTAVDMWSVGCIFAELINKQALFAGDSELQQLLHIFRLLGTPNEEMWPGVSRLKNWHEYPQWKPQQLSSAVSHLDENGLDLLNKMLRYEPSKRISAKEAMEHPYFDGLKKDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AP04</accession>
    <name>A0A166AP04_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AP04"/>
    <dbReference type="Gramene" id="KZN01574">
        <property type="protein sequence ID" value="KZN01574"/>
        <property type="gene ID" value="DCAR_010328"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211082"/>
    <dbReference type="OMA" id="DICDSTW"/>
    <dbReference type="OrthoDB" id="671172at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045283">
        <property type="entry name" value="AT3G44326-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33736:SF18">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33736">
        <property type="entry name" value="F-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="318" mass="35546" checksum="740F29BF3DD7389C" modified="2016-07-06" version="1">MTSNSANPESTFAALHSDIIETHLLTRFDGASLASVASTSNLLHALCDKQSLWKDICDSTWKSVQHPLVQEAISNFPGGYRSFYSDSFPVLGANSRQGKNHGLVENQTVEIISAVDIHYGKNPVFSRVAVTNTDASSFPGSLFSVDLIDRKETVEIPLKCEGFEKMSELEDKLRLSWIVIDPTLKRAANVSSLRPVSVRPHGDKRAVEVTYAAILSGKCCDIDTTEFVECRIVAVFGCEEGNNMEVRELSLCLVDMVRSRLNGELSLRILQEAMENGERRKESGQGKEMYAKSLDFKRQRFVFWVFFASFLISLFWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ARG4</accession>
    <name>A0A166ARG4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0311877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ARG4"/>
    <dbReference type="Gramene" id="KZN01684">
        <property type="protein sequence ID" value="KZN01684"/>
        <property type="gene ID" value="DCAR_010438"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211108"/>
    <dbReference type="OMA" id="VATADTW"/>
    <dbReference type="OrthoDB" id="30881at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF14">
        <property type="entry name" value="PROTEIN PGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="287" mass="30610" checksum="08EDDBF444A7A184" modified="2016-07-06" version="1">MEIPITQPVIAVLISSAIAARSYKKKSLDLSGALAGFLVMTIHFAVNYRFGAMLLVFFFTSSKLTKFGDEKKRKIDPEYKEGGQRNWLQVLSNAGIASLLVILFWVMAGSEDKCLDSTESKFATALIGSIIGHYSCSNGDTWSSELGILSDAQPRLITTFKPVRRGTNGGVTKAGLIAAAAAGSIIGLTFALMGFFTTNCMFDKSLNQLLVIPLSTLAGLSGSVIDSLLGATLQFTGFCSVRNKIVGKPGPTVKKISGLKILDNNAVNLVSIVLTAMLTSFAGLYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
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        <name evidence="9" type="ORF">DCAR_0313980</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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    </evidence>
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        <source>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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    </evidence>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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        <property type="project" value="EnsemblPlants"/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    <dbReference type="PANTHER" id="PTHR46327:SF9">
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        <source>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HDB9"/>
    <dbReference type="Gramene" id="KZN10110">
        <property type="protein sequence ID" value="KZN10110"/>
        <property type="gene ID" value="DCAR_002766"/>
    </dbReference>
    <dbReference type="OMA" id="HTANCLL"/>
    <dbReference type="OrthoDB" id="780646at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000287">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000390">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043891">
        <property type="entry name" value="SPARK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF62">
        <property type="entry name" value="OS05G0423500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19160">
        <property type="entry name" value="SPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="294"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG83724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="585" mass="65074" checksum="B3F7687754CEEFC5" modified="2016-07-06" version="1">MMSKFILFVVLNLCFVSSQSTSTSINCPLDFTILSHLSAASRNSTDVISQCKLILQGIRLVQSDYLRHSNSFLVSTAFTESCWQSLQSVFNEYPHSFNIRSTCGFQAQFITQGCNNITTRGQYEAKNSHLTLNNVVKSCKKTLQNRNFCAECSLATSGLGPSDSHEALPSDVKGEKVYYESDCVGYRWMYAAAFGNSYGPADRHTANCLLALTFDSPKSNKKHIKTITFVFLAFGFLILVMACVGFWLMWRYKLNMYKRQKKIFNSSPDLDMIGGDATLIKFTFSEVQKATNNFSLLNIIGRGKYGNVFKGVLPDGTEVAVKRFKNCSAAGDTSFAHEVEVIASVLHVNLVALRGYCIAITKYEGHQRIILFDLMKNGSLDDHLFGSTEKKLSWPIRQKIALGTARGLAYLHHGAQPCIIHRDVKASNILLDDDFEPKVADFGLAKFALEGVSHLSTHAAGTMGYVAPEYALLGQLTERSDVYSFGIVLLELLSGKKALFEHQDDQYTLLADWAWSLVRKGRPLDVVEEGIAELGPVEIMENYVFLAVICCHPQLYARPTMDQIVKILDSLSPVPVIPERPVSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HFU4</accession>
    <name>A0A166HFU4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_002851</name>
        <name evidence="14" type="ORF">DCAR_0102991</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HFU4"/>
    <dbReference type="STRING" id="79200.A0A166HFU4"/>
    <dbReference type="Gramene" id="KZN10195">
        <property type="protein sequence ID" value="KZN10195"/>
        <property type="gene ID" value="DCAR_002851"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212472"/>
    <dbReference type="OMA" id="RGKQRDQ"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF190">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
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            <dbReference type="ARBA" id="ARBA00001971"/>
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            <dbReference type="ARBA" id="ARBA00004167"/>
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            <dbReference type="ARBA" id="ARBA00010617"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="EMBL" id="KZN10195.1"/>
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    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="477" mass="54515" checksum="553C384979491FA3" modified="2016-07-06" version="1">MTIYFTTLFFIIFPIILFIFIRRKSARSSRLPPGSLGIPFIGQSLTLLSKMRSNTADKWVQERVQKYGPVSKLSLFGSPTVFLHGPAANKFIFSTSTSIISNQQTASIKMILGECNLFELSSEDHKRVRSALVPFLKPESLKRYTGKMDTEFRNHLKMYWEGKDQVTVLPLMKTLTFNIICSLLFGLESGPKKEKLIRLFQEMIEGMWSIPINLPFTNYNRSLRASAEVKQLVKELVCEKRIDLNLKGASSNQDLITSLLSVRDENNKEIISENEIVHNVMLIMVAGHDTSSVVITFLMRLLANNTEVYKSVLQEQENIAKDKSSGELLTWDDLVKMKYTWRVTQELLRVIPPVFGGFRKALKDIEYGGYLIPKGWQIFWATNMTHMDSTIFEEPSKFEPARFENQASIPPYCYIPFGGGARVCPGYEFARIEILVAIHYLVTRFTWELCCSDDSFKRDPMPVPTQGLPVKLIPKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDK3</accession>
    <name>A0A175YDK3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">NADH-quinone oxidoreductase subunit N</fullName>
            <ecNumber evidence="17">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">NADH dehydrogenase I subunit N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">NDH-1 subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ndhB</name>
        <name evidence="17" type="synonym">nuoN</name>
        <name evidence="20" type="ORF">DCAR_032530</name>
        <name evidence="21" type="ORF">DCAR_0C036320</name>
        <name evidence="22" type="ORF">DCAR_0C036336</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="20"/>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="17"/>
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        <property type="protein sequence ID" value="KZM81268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069476.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069491.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
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    <dbReference type="SMR" id="A0A175YDK3"/>
    <dbReference type="STRING" id="79200.A0A175YDK3"/>
    <dbReference type="GeneID" id="88614309"/>
    <dbReference type="GeneID" id="88614344"/>
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        <property type="protein sequence ID" value="KZM81268"/>
        <property type="gene ID" value="DCAR_032530"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM81283">
        <property type="protein sequence ID" value="KZM81283"/>
        <property type="gene ID" value="DCAR_032546"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614309"/>
    <dbReference type="KEGG" id="dcr:88614344"/>
    <dbReference type="OrthoDB" id="1876953at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Plastid Pltd"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045693">
        <property type="entry name" value="Ndh2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002701">
        <property type="entry name" value="PRK02504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22773">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19530">
        <property type="entry name" value="Ndh2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 17" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="5 20" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="7 17" id="KW-0874">Quinone</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="10 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)H-quinone oxidoreductase subunit 2 N-terminal" evidence="19">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="18">
        <location>
            <begin position="146"/>
            <end position="442"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF19530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KZM81268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOH16760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH16775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="56588" checksum="E637E7441EB0A1B7" modified="2016-09-07" version="1">MIWHVQNENFILDSTRIFMKAFHLLLFDGSLIVPECILIFGLILLLMIDSTSDQKDIPWLYFISSTSLVMSITALLFRWREEPVISFSGNFQTNNFNEIFQFLILLCSTLCIPLSVEYIECTEMAITEFLLFVLTATLGGMFLCGANDLITIFVAPECFSLCSYLLSGYTKKDVRSNEATMKYLLMGGASSSILVHGFSWLYGSSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFKLSPAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDIPFYFSSNEWHLLLETLAILSMILGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNDGYASMITYMLFYISMNLGTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLYLFWCGWQAGLYFLVLIGLLTSVVSIYYYLKIIKLLMTGRTQEITPHVRNYRRSPFRSNNSIELSMIVCVIASTIPGISMNPIIAIAQDTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE11</accession>
    <name>A0A175YE11_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_029489</name>
        <name evidence="4" type="ORF">DCAR_0933972</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YE11"/>
    <dbReference type="STRING" id="79200.A0A175YE11"/>
    <dbReference type="Gramene" id="KZM81876">
        <property type="protein sequence ID" value="KZM81876"/>
        <property type="gene ID" value="DCAR_029489"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200333"/>
    <dbReference type="OMA" id="PCWSPDE"/>
    <dbReference type="OrthoDB" id="1901794at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000152">
        <property type="entry name" value="Trihelix transcription factor ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF49">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE TRANSCRIPTION FACTOR MYB_SANT-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM81876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="329" mass="36676" checksum="C89ACD2A8193B9EF" modified="2016-09-07" version="1">MATPSLSPQDLPNPPLAPPANPSGPTNPRRLPPPCWSHDETVALIDSYREKWYSLRRGNLRACHWQEVADSVALRSPNEPGKTSVQCRHKMEKLRKRYRNEIQRLASAVGRNRYNSSWVHFERMDCMEKGPTAVALPVNRMTKIEDDVDEDEEEEEDSDIGFYPKSVRNGYGLGGNGNVGNGVNLCSWNRIPGQGNLVSPTPLAMYNKFDGYSTPKASEVPSFGGFKNGSVGRFGMGNNGNEGRVESKKESNDPAMELVGAIKALGDGFVRMERMKMDMVREVEAMRMDMEVKKTEMILESQQRILESFVNAVSEGSNKKAKRMSSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGN2</accession>
    <name>A0A175YGN2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carboxypeptidase</fullName>
            <ecNumber evidence="8">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0935103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="8"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGN2"/>
    <dbReference type="Gramene" id="KZM82653">
        <property type="protein sequence ID" value="KZM82653"/>
        <property type="gene ID" value="DCAR_030222"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200870"/>
    <dbReference type="OMA" id="FHCTIST"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000163">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF454">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620724" description="Carboxypeptidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH15561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="457" mass="50717" checksum="91884FDFDEB2EE77" modified="2016-09-07" version="1">MQSVSTKLLLLLSILNQYYLLATPSRVHSISPIPNEALPTKSGYLQVNSTTKSAMFYTFYEAKEPNLLLSETPILIWLQGGPGCSSMLGNFYELGPWRVVSSQGHNVEKLALEPNQGSWNRLFGLVFIDNPIGTGFSLATTTEEIPRDQQAVSKHLYKAIRAFIALESLFKTRPIYVTGESYAGKYVPAIGYYILKRNAGLPTSSQVNLVGVAIGNGLIDPITQVATHAMSAYFSGLVNEKQKTVLEKVQDEAIGLAKLGNWSVATDARSKVLNLLQNMTGLATLYDFRRSIPYEEDLVTTYLSSMEVKKALGANVSIDFEVCSDTVGKILHEDLMKSTKYMVEFLVKHTKVLLYQGEFDLRVGVVAAEAWMKKMKWDQIENFLEADRKVWKVKGKLAGYVQKWGSLSHAIVSNAGHLVPADQAVNSQAMIEDWVLNRGLFSDNGIVEHLNPSYNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YK50</accession>
    <name>A0A175YK50_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_028633</name>
        <name evidence="3" type="ORF">DCAR_0830701</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
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                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3H1"/>
    <dbReference type="KEGG" id="dcr:108204445"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000053">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055183">
        <property type="entry name" value="PTEN2A/B_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF96">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND PROTEIN-TYROSINE-PHOSPHATASE PTEN2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22918">
        <property type="entry name" value="PTEN2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="8">
        <location>
            <begin position="135"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="228"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="9">
        <location>
            <begin position="321"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="477"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="67245" checksum="AA895911529795E9" modified="2024-05-29" version="1">MDSELDDASDTQPLKAPEVQTQSGVELDNPAGNSRSMVSVSGLSMWAKNLKIPDSLVGKQDSSPSDKSPLARFTSGLGFRASPKASRLDDSAEGTSPSAQSNLFGTITKGLVDSSKVAVKAVQVKARHVVSQNKRRYQEGGFDLDLTYITENIIAMGFPAGDMSSGFFGFVEGFYRNHMEEVIKFLETYHKGRYKVYNLCSERLYDASLFEGKVACFPFDDHNCPPIQLIISFCRSAYSWLKQDIENVVVVHCKAGMARTGLMISSLLLYLKFFPTAEESIDYYNQKRCFDGKGLVLPSQIRYVKYFEHVLSFSNGENHPVRRCILRGLRLHRCPYWIRPSITISDHNGVLFSTKQHPKTKNLSPEDFWFSAPRKGVMVFALPGEPGLTELAGDFKIHFHDGQGDFYCWLNTSMFETRKFLTTDGLDGFDKRKLPSPGFQLEVVVVEYSGTVPNTTMGTQTETSTNGLTERPGANHAPTGGAVGATAAPQTVRDSVSSEKNDDVFSDTDTEAEDVNSSKHRQAGLASEADVASVNTTSSTQALSKSDQISNLSHDARQLAPGKTSFNEPKDSTGTVVLGDKVSSPVGEVSDFKAMAADASVFTFGDEEDFESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W456</accession>
    <name>A0AAF0W456_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0102065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
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    </dbReference>
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        <property type="entry name" value="KH_dom"/>
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        <property type="entry name" value="ZFP36-like"/>
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    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF154">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG82895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="303" mass="32065" checksum="2B8F1D5BD61EB1B0" modified="2024-05-29" version="1">MDFGSARKRARPEAPALNGNAAFKKSKQETESFTTGIGSKSKPCTKFFSTAGCPFGEGCHFLHYVPGGIKAVAQMTGNNPAAPARNHGQPSFGQPSFPDGSSPPAIKSRLCNKYNSAEGCKFGDKCHFAHGEWELGRPTVPYHEDPRGMGPMPGSRFGSRMEPTPPSYGTGTSFGANATAKISVDASLAGAIIGKNGVNSKQICRQTGAKLSIREHEADPNLRNIELEGTFDQIKQASQMVQELIVSIGSASGPPPKSHAMSGTGPANNFKTKLCENFAKGSCTFGDRCHFAHGAEDLRKSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4H5</accession>
    <name>A0AAF0W4H5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0102004</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4H5"/>
    <dbReference type="KEGG" id="dcr:108192244"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF783">
        <property type="entry name" value="MALECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042105502" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="774"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="112"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG82836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="90517" checksum="B742AEFE1B3DF0F7" modified="2024-05-29" version="1">MGKPLSLITPLVLSIFILMFMKCSSCPAHQKQSLLHFKASILTIFNSSNSSSETSFFSLDSWDSTSDCCNWERVICSPRTREITALYLDSLLLMPSDQPIVVDSRILDPVFRIRSLMFLDVSLNNIQGEISGEGLANLSKLVHLDMRQNGLVGSIPRQVFHLRFLRFLDLSSNSLKGGLSGEVGRLGNLRTCKLDENFLGGDIPAEIGNLTKLQQFSVRQNQFSGGIPDSILDLKELEVLDLRSNQLVMQIPREIGSLSNISTLALSKNFFTGVIPPSIRNLSKLETLRLEDNRFSGDIPAWLFDMESLKNLFLGGNQMIWNNNVKIVPKCLLSQLSLKSCKIAGEIPEWISTQKNLDFLELSDNELTGRFPLWLSEMEVGSILLSDNRLTGSIPSRLFQSLSLSILALSRNNFSGELPENIGEANEIMILMLSGNNFSGPIPKSIADIYRLLLLDLSGNKFSGNTFPIFDPDGLLAYVDLSSNELSGDIPVSFCTETKILALGNNKFSGKLPRNFTNMNQLEHLDLHDNNITGEYPEFLSQMSSLQVLNLRNNSLHGSLPSKSFSNQSNLRILDLSNNNLGGSIPSELGNLMGMIETPSTSSLTTDMFTFSIEFKDLIVNWKKAIRGLSSRRLDIYSLLDLSNNKFSGNIPSSLGNLKGLKLLSISNNRLSGNIPQSFGDLESVETLDLSNNNISGVIPQSFTRLKQLSVLDVSNNKLSGRIPQGGQMDLMNVLNYFANNSGLCGVQIGVKCPDDQLTPEADEEGDDSEKEPWFLWKGALIGFSLGFVFSVLMAFLGGYFVLPPPPKPKYHSVKYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4T0</accession>
    <name>A0AAF0W4T0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger LSD1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0100776</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4T0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040319">
        <property type="entry name" value="LSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005735">
        <property type="entry name" value="Znf_LSD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747:SF3">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06943">
        <property type="entry name" value="zf-LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG81625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="173" mass="18135" checksum="6AB8A18B7E440481" modified="2024-05-29" version="1">MQSQLVCRGCRTMLLYPQGAANVCCAVCSVVTTVPPPGMDMSQLICGGCRTLLMYTRGASSVRCSCCHTVSLVPAPNHQVAHINCGNCTTTLMYPYGSQSVRCAICQYVTNINMGNTNIPVPASRPDGTASSGTTTATSTLVFSQTQTVIVQNPMSVDESGKTVNNVVVGVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9R2</accession>
    <name>A0AAF0W9R2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0103400</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9R2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="X8" evidence="16">
        <location>
            <begin position="297"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG84218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="385" mass="41454" checksum="9EE9A67BFA6B7902" modified="2024-05-29" version="1">MLTALSDTGIEVIVGVTNEEVLGIGESAAVAAAWINNNVAAYLPSTNITAIAVGSEVLSSIPNAAPVLVPAMNNLHKALVASNLNNHIKVSAPQSMDIIPKPFPPSTAMFNSTWNSTISQMLQFLKTTDSYFMLNAYPYYGYINNGGIFPIDYALFRPMSSVKQIVDPNTLFHYDSMFDAMVDATYNSISAFNSSIIPVVVTESGWPWFGGANEPDATMDNAETFNNNLIQRVSNDSGPPSQPTIPINAYIYELFNEDKKSGPISERNWGLFFSNGTSVYPLSLTTSNNSVGNSSVAFCVAREGADSSKLQSGVSWACGQGQANCTAIQSGNPCYLPDTLQNHASFAYNDYYQKMKSIGGTCDFDGTAMTTKNDPSYGSCIFTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBV0</accession>
    <name>A0AAF0WBV0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBV0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36723">
        <property type="entry name" value="F22C12.19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36723:SF1">
        <property type="entry name" value="F22C12.19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="623" mass="68757" checksum="59C76665CBEF99C4" modified="2024-05-29" version="1">MGSESCGGVGVKDYRSGVVSVDFSSRIDSSNSFSTPNEAAWNKMVNGEGCKNPIEKASKELNFTRTSNPLTFQDLEAKKQRRTSGKAARKSRLSQLEVSMNETETNDVEGKAKEVGFFYGKGITLEKTQTSRQKTNINGKRNDKRNGKAPKSKYDSFSVKTGLASFSSAALGNNILGGYGLRADMEDVTKNIDELSLNELLEGSYKCPKFAMDKGERSEDPDDKIMQSVKNAWSILQTRKIKPQAMEVDGSYHQEVLSPCYLALDSLVGNRKEEANDTDIIDVSPAHKESGIQSDILDFPLHKPEKIFERLTLPPPRDLEFLLLDTAKSATPRISADPRPGKPVNQRGCLPPFSWSNAYSGHCKSNVDPVKLSTSRSTCQSRWAKIETTFPSPDTTSGFLTELESLRYDDSIVPVEYQSLAPVVKEKNQPESVSLTGQGLIKCTVAPQASRGLSSPRALAAAQLLCDISAHPLKKNDLGMIKSLKRPSQKSMRAPDCKPSEKPENIYATPITKSRFDCPPSNPEVVFGLKRPRPSVKERSAETLHNNITPRGQLPSWSMPRSNRSSPNKSLRGSVSETKPYNVVKKPFMLPPVARTMEAASSSQPKQRKLMQIGWNRESGKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDA2</accession>
    <name>A0AAF0WDA2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RSE1/DDB1/CPSF1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
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        <location>
            <begin position="94"/>
            <end position="245"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="280" mass="31209" checksum="B3EEE7D963C817B5" modified="2024-05-29" version="1">MYKGRMLLDEGVYSFVSYSLYHSFMCHTFACFFQISEVYSKELKGAISAVASLQGHLLIASGPKVILHKWTGSDLTGVAFYDAPPLYVVSLNIVKFLIDGSTLSLTVSDDQKNVQIFYYAPKMSESWKGQKLLSRAEFHVGAHVTKFLRLQMLPTPDRTNVAAVPDKTNRFALLFGTLDGSVGCIAPLDELTFRRLQSLQKKLVESVPHVAGMNPRSFRQFHSKGKAHRPGPDSIVDCELLCQFEMLVLEQQHEIANQIGTTRSQIVSNLNDLALGTSFL</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
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        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="67"/>
            <end position="321"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG89091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="374" mass="42605" checksum="739CA6C92355ECEC" modified="2024-05-29" version="1">MAIKDLAAPHKNVAVIFGSTGLVGRELARRLLSRGGWKVYGVARKADTTNIKDSNYQFISCDLLQPAETRKKLSWLHDVTHIFWITWASEFPLDSRECCEQNKAMMSNALNALLPTAKGLRHFSLQTGVKHYISLQGPFDGNEVRYYHEECPRLSTENNFYYTLEDLLKERLGGKVAWSVHRPGLILGNSQRALYNFMGSLCVYGTICKHLNLPFMFGGSRECWEEMCIDGSDVRLVAEQQIWSATNDALLSTDGHAFNAINGPRFTWKQIWRKIGLKLGAVVPEDMFSEDFMFSKFMADKGSVWKEIVAKEGLVGTEMEDLANFQFLDHLFRCPAKMLATRDKADRLGLTAMYETLDSILYWVDDMRKDKLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGU1</accession>
    <name>A0AAF0WGU1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ORF">DCAR_0207141</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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            <end position="269"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="269" mass="31026" checksum="7561CFFFC0D94657" modified="2024-05-29" version="1">MILGVVEEDFPVSYCRWKTLEVQCGNDQSIRQLAVSKGDNSKLPELIFNRLQLSDQEYCGLLRRNFGNFVAREAVLDEEYWVAAWLRAEAHWESLSYMRHVDSYKRKYAEQEFYALKRRCCRQEGKSLKCCCFVAVKKEDKNVRRTVLNSVVGTMDLSIRQFVQGETYPGEVNRLSAVLASQEPFDAHKYAYIANVCVSKYARRQKIASNMVYLATDMATSECMKQLFVHVNVDNKPAQELYKKTGFKIVSAASTPLSKDQRLLMCMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WIL8</accession>
    <name>A0AAF0WIL8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="14">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0209480</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108208982"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <source>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKM6</accession>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZNHIT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 2 PROTEIN FON -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555:SF0">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="2">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="419" mass="46985" checksum="9EDE0E8737E03FDD" modified="2024-05-29" version="1">MSESTSIPEAQRLAPSLLNPSSRIICRVCEKQFSQYTCPRCNTRYCSLHCYKSHSRSCTESFMRDNVVEELKQLEPNDATKQKMMAILQRFHTQEEIDNSDELLDSTPGDVTDSNFSEVVIQKILSGGEIQVEDLSEDEKKRFQRAVVSGELSRMIEPWEPWWLKPSAKTIHLSEQGSRLVQPIVRHESIASKSEIVENDQSLHIPPGPETPLPPVSKLSATAPSPLLAVHLVDIIYSYCFTLRLYNGEWLTDPIGSVMHALSVSLVLGQGGKPETVSEAVSHCLEQTCSPVLRHVGGLQFGLGLLDDVVCLLSIGGTALVCLLCDFQRMIQAAEKELKSEKPRKSRRVEAKGNLKSAERKVYFMMCWVHEQHEDTWFSIASMVEAEKSANLACTGNRKDTLSVQERARPRGKMLIEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN57</accession>
    <name>A0AAF0WN57_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0311672</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN57"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140566">
        <property type="term" value="F:histone reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056280">
        <property type="entry name" value="AIPP2-like_SPOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049914">
        <property type="entry name" value="PHD1-3/5-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33304">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33304:SF18">
        <property type="entry name" value="CHROMATIN REGULATOR PHD FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23121">
        <property type="entry name" value="SPOC_AIPP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG92406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="24356" checksum="AEFCF105F32EB493" modified="2024-05-29" version="1">MATTCLKCGSQSWDNAFVCCSRCEDCVVHRYCLDVIPNTLDEIVTWYCEDCQEDLTPSTKEAHGSGSMNIRDTNSTDTEDHMGAFVIINKKRGTYDGVVAHISNKACSKVCEKARSLENFLHFEKHPKSDVWPKSFQNSLPSDESIALYFLPADRSGEEVFENLVDYMIGDEIALKATIEDAELLVFSSTELPLSYWRFQGKYYLWGVFRGKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WP99</accession>
    <name>A0AAF0WP99_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0312908</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cdt1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WP99"/>
    <dbReference type="KEGG" id="dcr:108210773"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071163">
        <property type="term" value="P:DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08674">
        <property type="entry name" value="Cdt1_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1420">
        <property type="entry name" value="DNA replication factor Cdt1, C-terminal WH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045173">
        <property type="entry name" value="Cdt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038090">
        <property type="entry name" value="Cdt1_C_WH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637">
        <property type="entry name" value="DNA REPLICATION FACTOR CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28637:SF13">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01075">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="4">
        <location>
            <begin position="55"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="56982" checksum="F8D9C5E9E1FDBC69" modified="2024-05-29" version="1">MEQIKSEQIRKTVDAQNFDSTTVKSSDLQSDVLSRTPAKANETLPSRDRREATELPQKYKTMAEVFNRMTSSLRLLSLRKKSPTFKNVSSQVQILARREFSYKHLAQIMYILPEAVKTDRILIHNEKTMCMEPEMKITLLFDIVKGHDEGSDYLALFVLFTSRLFDFLSANTEGCEIPEAELPEPFNKQSINITEDSLTLDSSTVPTPTLGEAELSNASNLPSSFSRHFSQKAITERTQLLASADPLLPTKCDSLTQEDTEPQKQSPASCVDSTISTNPVELTKPPCFNPITCENTPIKFTSEKENLMVETPAQMTPQRSLPSCDVKLKTGSVLQKTTSSLSAKRTLDFSYSEGKGRILDFGDDITEHGEVAHNTRPGRETTDVVENETACLAVPPVKVHSCTSDNSIFNQSGSTSRKQILSCLPDIVVVVNNIFQCADRTSITKEELVHKIIMNNLDIVERRVVEEQIDLLEKLVPDWIGKKLAPSGDILYNIKKVSDLKSISERLICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPJ9</accession>
    <name>A0AAF0WPJ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0313059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPJ9"/>
    <dbReference type="KEGG" id="dcr:108211510"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="single-stranded DNA-binding protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF16">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23332" checksum="B38B435D043C23E2" modified="2024-05-29" version="1">MKMAYSLSRTLCRSVLSNPTKSFIPVSSSSSYCTHEFNSNENTAAASAQVEGDDVSQRPVYVDRPLENGLDYGIYKAILVGQVGQNPVQKKLRSGRTVTLLSIGTGGIRNNRRPLENEEPKDYANRCAVQWHRVSIYREKLGEVAIKHVTPGSILYLEGNLETKVFSDPVTGLVRRIREVAIRQTGRLVFLGQGNNSQKSLPGEVKGVGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQD0</accession>
    <name>A0AAF0WQD0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0312093</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214497"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000923">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056228">
        <property type="entry name" value="ABCC10-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF369">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24358">
        <property type="entry name" value="ABCC10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="914"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="954"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1052"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="308"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="625"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="952"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1231"/>
            <end position="1465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG92816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1477" mass="165075" checksum="6350E4B339731928" modified="2024-05-29" version="1">MDELWTLFCGKSDCSDGNHGNCGSDLVLLTHPSSCINHVLIFLFDILLLAMFLLNIFFKRPDKSTHSSRVSRLQWISAIYNGVLGLGYLCWGVLTLAERLRRKQAIVPLNWWLLALFHGLTWLIVSLAVSFWGKRFSRAPVRILSVLAFISAGVSFGLSLFAAITHKELAMKIALDVFSFLGASLLLLCTYRGLIDEESCENESYLYTPLNGDRNAADMKNDSAGFVTPLATAGFFSKMSFWWLNPLMKRGRQKTLLDEDIPKLRKEDQAETCYLLFMEQLNKQKAKDPRNQPSILRTIFLCHKRDTFISGFFALIKIITISAGPLLLNAFINVAEGKGSFKYEGYVLALLLFFSKNLESLSQRQWYFRSRLIGVKIRSLLTAAIYKKQLRLSNAAKTVHSAGEIMNYVTVDAYRIGEFPFWFHQLWTTSLQLCFALVILYRSVGLATIASLVAIILTVLCNTPLAKLQHKFQSKLMVAQDERLKASSEALVNMKVLKLYAWETHFKNAIEKLRTIEYKWLSAVQLRRAYNSFLFWSSPVLVSAATFGACYFLEIPLYASNVFTFVATLRLVQDPVRCIPDVIGVVIQAKVAFARILNFLEAPELDSSKIRCKWNRENINHNIIIKSADLSWEENKSKPTIRNINLEVKMGEKVAICGEVGSGKSTLLAAILGEVPSVNGTMQVYGNIAYVSQSAWIQTGSIRENILFGSSLDYDRYQDTLDKCSLVKDLELLPHGDLTEIGERGVNVSGGQKQRIQLARALYQDADIYLLDDPFSAVDAHTATSLFNEYVMDALSGKTVLLVTHQVDFLPAFDSVLLMSDGEILHAAPYHLLMASSREFQDLVDAHKKTAGSDRISELNSSQERESVSRDVKTCPEKKLITPGDDQLIKQEEREVGDAGLKPYIQYINQDRGLLLFSLSIVSHIAFVTCQILQNSWMAANVENPEISTLRLILVYLVIGFTSTIFLFSRSLASVFSGLHSSKSIFSKLLESLFRAPMSFYDSTPLGRILSRVSADLSIVDIDIPFSLIFSVAASINAFSNLGVITVVTWEVLFVAIPMIYLAILLQRYYFSSAKELMRINGTTKSLVANHLAESLAGAMTIRAFEEEDRFFLKNLDLIDTNASPYLHNFAANEWLIQRLETLSATVLSSSALCMVLLPSGTFSSGFIGMALSYGLSLNLSLVGSINNQCLLANHIISVERLKQYMHIPSEAPEVIEDKRPPINWPAVGKVDIQDLQIKYRPAAPLVLRGISCTFEGGNKIGIVGRTGSGKTTLIGALFRLVEPTGGKIVVDGIDISTIGLHDLRSRFGIIPQDPTLFNGTVRYNLDPLSQHTDQEIWEVLGKCQLRETVLEKEVGLDSLVVEDGLNWSMGQRQLFCLGRALLRRSKVLVLDEATASIDNATDLILQKTIRTEFADCTVITVAHRIPTVMDCTMVLAISDGKLVEFDKPMNLMKKDGSLFGRLVKEYWSHYHSAESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WR21</accession>
    <name>A0AAF0WR21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0313863</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WR21"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF1">
        <property type="entry name" value="CYTOCHROME P450 82E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG94567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="58117" checksum="9911D60866967FF4" modified="2024-05-29" version="1">MNLKLYFLTFWSSRNKNGGEEKKKQAPKANGAWPILGHLPLLSGPDPFRILADMADEYVPVFRVQLGLQHALVVSSKEAIMQTTNDMSFMGRPNVLALKYMGFYGGFYALAPYGPFWQEMRKVSILELLSNTRVELLKPVRASEMSTCISELYSLCCRDGKVGSVKVAIDKWFQQVLFNMIAQVIARKRYSSVGKDASDKELKCLKRAYEDFFVMLVTFQKSKGIPFTRWMNFQENRVMKRTEKEFDFILSSWIADHKQRRGIQGQLKEDRDFIDIMLSMFEGSDDFIQGHKTDDVIKANISGIIFAGTDANYATITWALALVLKHREVLKKAQEELDLHVGQERWVEESDIKELKYLQAILKETFRLYPAGPLSILREALEDTTVAGHYVPKGTQLLANIWKLHRDPGTWTDPDEFQPERFLTTHAGLDVKNQQYELIPFSTGKRSCPGMATGTQMILLTLARLLQGFNLVTPTNEPINMTEAAGITLHMKYSLEVVLTPRLPNKLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRF5</accession>
    <name>A0AAF0WRF5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0313286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRF5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006691">
        <property type="term" value="P:leukotriene metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050997">
        <property type="entry name" value="MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250:SF22">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG93996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="73" mass="8479" checksum="0E9B5EC99A579B8A" modified="2024-05-29" version="1">MVQRGHQNSLEWMPLFLTFMTLGVIKHPDISSVLGVVYIFSRYFYFKGYSTGDPKKRLSVGNNIFLWSIFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTX2</accession>
    <name>A0AAF0WTX2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS4c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0415054</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTX2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1050.10:FF:000002">
        <property type="entry name" value="30S ribosomal protein S4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1050.10">
        <property type="entry name" value="Ribosomal Protein S4 Delta 41, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="112" mass="13251" checksum="F6E892BF3A4D48F1" modified="2024-05-29" version="1">MSRYRGPRFKKIRRLGVLPGLTNKRPRAGSDLRNQSRFDKKSQYRIRLEEKQKLCFHYDFTERQLLKYVRIAGKTKGSTGQVLFQLLKMHLDNILFRLGMASLKITSFIAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WV99</accession>
    <name>A0AAF0WV99_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WV99"/>
    <dbReference type="KEGG" id="dcr:108215783"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002340">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF74">
        <property type="entry name" value="OS02G0658500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="478" mass="52828" checksum="556EE640EA3531C1" modified="2024-05-29" version="1">MPSSPRKSPFPPLKNPLKHVFHKMRFHSRSNSVTPPTEFDETHVVNPVYNPGLTRVSSGPDCTSLISDENLLCILSKVDEKQLGVCGLVCKRWEKLSGKLVRSLKLLDWEFLDSGRLIYRFPNLVDVDVVRACVKRSHSSGIVLSSNFLSVELNSGVLNDGFVKKLDLLDSGVVDQGIRVLVEGCLSLQKLVLVNVSEEGLSCVAEGCKTLQELELYCCCDFVLRGISKCRNLQILKLVGCIDGFYDSVVSDIGLTILAQGCRRLVQLELVGCEGSFDGIKAVGQCCQMLEELTLCNHRMDGGWLAALSYCGNLKTLKLKSCKSIDSCPGPDEHLSYCPTLEELHLQHCQMRDKQGVRALFIACQTVRDLVIEDCWGLDNITFAYASICRRVRLFSVEGCSLLTMDGLDPVVLAWKELNRLKVISCNNIKDGEITLELATLFSSLKELKWSPDSRSILLSGLAGTGVGQRGGRSFSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY40</accession>
    <name>A0AAF0WY40_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glycerol-1-phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0416201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sn-glycerol 1-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>sn-glycerol 3-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:66372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HAD-like hydrolase superfamily. DOG/GPP family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.21" evidence="5"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY40"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG96863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="26567" checksum="2487E2FCE34CAFC7" modified="2024-05-29" version="1">MTTPEPSSKSITHVVFDMDGLLLDTEKFYTEVQEMILARYNKTFDWSLKAKMMGMKAIEAAKVFVNETGISDSLSAEDFLVEREETLQKLFPTTDLMPGADRLIRHLHANKVPICVATGSKKYHYELKTQRHQELFSLMHHIVNGDDPEVKQGKPAPDIFLAAARRFEGGPVDPKKVLVFEDAPSGVLAAKNAGMSVVMVPDPRLDSSYLDTADKILKSLLEFQPADWGLPPFVDETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYW2</accession>
    <name>A0AAF0WYW2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0417495</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYW2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14335">
        <property type="entry name" value="UBA_SnRK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001222">
        <property type="entry name" value="Serine/threonine-protein kinase ppk25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="291"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="16">
        <location>
            <begin position="442"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG98154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="491" mass="55681" checksum="28C8BB5B349E1710" modified="2024-05-29" version="1">MDGSIAKRTKSSRVVLPNYKIGKTLGIGAFGKVKLAHHILTGIKVAVKILERQSIDEKAAEKVRREINILRLLSHPHVVRLFEVIETPTTIYMVMEYMDSGELFDHITENGRLNEAEARYFFQQIISGVESCHLHMVVHRDLKPENLLLDKKRNVKIADFGLGNVMRDGHFLKTSCGSPNYASPEVITEQLYAGPEVDVWSCGIILFALLCARLPFDDDNLPGLYAKIKSGIYTFPTHLSVGARDLIARILITDPTIRISIPEIHKHPWFQQQLPLHIAVRPKIVTCDPKEINEDIFKEMVKSGFDSFELINSLQNGVQNKGTVTYYLLLHNRFGAQGSHQNNELSRGLECTDRPEVYLRSPPQLKTQWALGFRSTASPHETMIDVLKVFESLNVRWKKIGNYNMRCLLLSHLSKQATLMDGPPIKDIYGNELIITSLRSKALKSHDAVKFEIQLYKATPELYVLDLQRIYGPPFLFLELCASFFSLVVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZJ1</accession>
    <name>A0AAF0WZJ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Lipid droplet-binding protein CGI-58 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0417132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108219483"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Probable 1-acylglycerol-3-phosphate O-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="13">
        <location>
            <begin position="120"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="415" mass="46407" checksum="355E9E8AE420010C" modified="2024-05-29" version="1">MKLGPRLIRNKPGAQLKSSNSILSSAAAMAEEISSSSTTAATRTKKSSRWPSLLRWIPTSTDHIIAAEKRLLSLIKTPYTQENVNIGSGPPGSKVRWFRSTSNEPRFINTVTFDSKEDSPTLVMVHGYGASQGFFFRNFDFLAKHFKVIAIDQLGWGGSSRPDFTCKSTEETEAWFIDSLEEWRKSKNLSNFILLGHSFGGYVAAKYALKHPEHIKHLVLVGSAGFSQESEQSERLTRFRATWKGAIINHLWESNFTPQKIIRGLGPWGPDLVRKYTTARFGSYSTGDVLTEDESKLLTDYVYHTLAAKASGELCLKYIFSFGAFARSPLVQSAADWKVPTSFIYGFDDWMSYQGAQEARKNMNVPCDIIRVPQGGHFVFIDNPSGFHSAVLYACRRFISCDPDNYPFPDGLTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X004</accession>
    <name>A0AAF0X004_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0518578</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X004"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF34">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="9">
        <location>
            <begin position="55"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG99230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="158" mass="18057" checksum="6E41A19B35178805" modified="2024-05-29" version="1">MSRESITSAINYEETELTLGLPGESRGTKRAFSEETMMVGWPPVKEHRKIVMRSCKYVKVAVDGTPFLRKVDLQSYGSYDELLRALQDLFGCFVVRDLVDEKKLVDVFNGKVEYVPTYEDRDGDWMLLGDVPWKMFSQSCKRLRLMTGTLYIGSSSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X2C0</accession>
    <name>A0AAF0X2C0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0519632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2C0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH00273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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</entry>
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                <person name="Iovene M."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Macko-Podgorni A."/>
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    </evidence>
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        <source>
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                <person name="Senalik D."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XK42"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38382">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38382:SF1">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH08289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="19049" checksum="3462D08EBF08AD2B" modified="2024-05-29" version="1">MRVSLSSFLDKKLQKGASLSKSVQGKQKPFSSPVIGVGVSGCREGENIGNRTGEPGLNCDIDVAFEKLKSTADKKEGFISLPANDFGGSNIHHSQGSRKRGNSFEGKDEKATARKVLVTLGDNNKNAYNPRKKQFISYEKSKPLFNHSAGGTVTWKALTMKKWVATKCGKGSDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKG0</accession>
    <name>A0AAF0XKG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="4">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein HEAVY METAL ATPASE 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0728174</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Cu(+)(in) + ATP + H2O = Cu(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKG0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000033">
        <property type="entry name" value="Copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000031">
        <property type="entry name" value="Probable copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF2">
        <property type="entry name" value="COPPER-TRANSPORTING ATPASE HMA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="913"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="942"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="20">
        <location>
            <begin position="44"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="20">
        <location>
            <begin position="118"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="20">
        <location>
            <begin position="193"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOH08727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="974" mass="105409" checksum="A80CE36260F8F555" modified="2024-05-29" version="1">MIALLTSLNTMIFRTKMEINGNDELKSPLLQPSDVTLNADRTTKSIMFKVSGIECASCATSIESALEKLSGVESVMVSPLQGQAVVRYIPEQITAKKIKETIEDAGFEVNDFPEKDIAVCRLRIKGMACTNCSESAERALSMVDGVKKAVVGLALEEAKVNYDPNITDTDQIIKAIEDAGFGADLISSGSDVTKVHLDLDGISSPEDLSVIHCVLESQEGVQYVEMNLEEHKVTVSYDPDIVGPRSLIRCIHEAGQGEKSYHARLYNPPRQRETEKKHEIQMYRNQFWWSCIFTVPVLMFSMVLPMLPPYGNWLDYKVHNMLTLGMLLRWFFSSPVQFIIGRRFYIGSYHALRRKSANMDVLVALGTNAAYLYSVYVLVKALTSATFTGNDFFETSSMLISFILLGKYLEVVAKGRTSDALAKLTDLAPDTAHLLTYGADGNVISEEEISTQLIQRDDILKIVPGAKVPADGIIVDGQSHVNESMITGEARPVAKRPGDKVIGGTMNENGCLLVKATHVGSETALSQIVQIVEAAQLARAPVQKLADQISKIFVPAVVVAAFATWLGWFIPGEAGFYPKSWIPEAMDAFELALQFGISVLVVACPCALGLATPTAVMVATGKGASQGVLIKGGDALEKAHKVKIVVFDKTGTLTAGKPTVVSAKIFSKFSMEEFCNVVIAVEANSEHPIAKAVVEHAKKFRKEYGSQTEQSSEVKDFEVHPGAGVGGKVGERMILIGNRRLMWASNVPVLPEVDAYISENEELARTCILVSINGRLAGALAVTDPVKPEAARVISFLQSMSITCIMVTGDNYATATAIGKEVGIQEVFAETDPLGKADRIKELQMKGTTVAMVGDGINDSPALVAADVGLAIGAGTDVAIEAADIVLIKSNLEDVITAIDLSRKTISRIRLNYVWALGYNILGMPIAAGILFPFTGIRLPPWLAGACMAASSISVVCSSLLLQFYKKPLHRNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XM76</accession>
    <name>A0AAF0XM76_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0729026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XM76"/>
    <dbReference type="KEGG" id="dcr:108193307"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.410">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43116:SF3">
        <property type="entry name" value="CLASS I PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43116">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="3">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="480" mass="53851" checksum="01B17B4148E352BF" modified="2024-05-29" version="1">MSYLILKRSFRRLISSSSSIHFQNPSFKSQCHHFCSSHLKNTIQASGYHSFWKRCRGSVLFSANYLKISSFGPSERFFGSGAAVELSSSDGLTVEGIIAKQWPIVEESVSDWKSHASSIAQSIHLIKKRLKWKKLEVRLAILSSQLEKPDLWDDPIHAGKINREHGSLVNKVKAIKVYEQELFEHIDMIKLAREENDPNMESESMKALASMRKSIKEKELEALLAGEHDSCSCFIEVQAGAGGTESMDWASMVMQMYKMWAQHRGYRVTVVDVMPGEMAGIKRATIKVDGEYAFGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILDDGATTHFQINESDLRVERMRAGGAGGQHVNTTESAVRITHIPTGISASCQNERSQHQNKASAMSVLQSRLDQLEIVRHAQANAQHTQSLNDISWGNQIRSYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDAFIFSFLSASMDKDEDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMM0</accession>
    <name>A0AAF0XMM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0730301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMM0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH10828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="306" mass="33897" checksum="2ED48AD9ACDCC297" modified="2024-05-29" version="1">MHDLLKEEFVLSTADSVAPCIIGSSPWWTSAKSHIDNSYAEQSCLSKSLSLKMAAQSSYCQSFKQLEFRFQDLDLSPTQSSSQSYPEVAGAAASNLCGQDMISTKVPLSYPDPYCHGLPSTGTEPMEVYHPHITAAASARVPLPNDFSQDEPIYVNAKQYGAILRRREYRAKLEAQKKLIRNRKPYLHESRHLHALKRARGSGGRFLNKKKLEDSNPISESTQSQTCSTILKTELHCSPKTYDINPSPTGWCYDMMTISDNDSCSIFQQQETSFSSYPAHVGMDLRGGKPTKSCSSLEFGGISSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMM8</accession>
    <name>A0AAF0XMM8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 25 member 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0729253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH09794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF3">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="26"/>
            <end position="110"/>
        </location>
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        <location>
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        </location>
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            <begin position="233"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="35421" checksum="543B79035A43DDF6" modified="2024-05-29" version="1">MSSAAVAASQTRPFDQTEINWDKLDKTKFYAVGAGIFSGITVALYPISVVKTRLQVVSKNSTERNAFSVIRGLLKAEGIPGLYRGFSTVITGAIPARVIFLTALETTKVAAFSVVEPLKVSEPTQAAIANGVAGLAASLLSQAVVVPIDVISQKLMVQGYSGHASYNGGLDVARKVLRTDGIRGLYRGFGLSVMTYSPTSAVWWATYGSSQRAIWRFLGHGSESDSPPPSQWEIMSVQTGGGIFAGATASCITTPLDTIKTRLQVMGNERRPTTKQVIKQLIADDGWLGLYRGLGPRFFSMSLWGTSMILAYEFLKRSCTQEYLTHCN</sequence>
</entry>
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            <fullName evidence="1">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0832071</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF14291">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="6"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="227" mass="25461" checksum="08484AFCD4A851B3" modified="2024-05-29" version="1">MNPKRHIDVLLSQQSEQVKNDYRIQLTSAIDCIRFLLLQGLAFRGHDESKESKNRGNFLELLNFLSCHNEKIESVFKNASDNNKLTAPSIQKDITNACSVLTLKAIILELGEEPFAVLVDEARDVSYKEQMAVALRYVNERGCVLERFIGIVHVSDTTAISLKAAIESIFASLGLSISRLRGQGYDGASNMQGQFNGLKSIIFQGQELLADTHFCRKIIYFWLGCDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR78</accession>
    <name>A0AAF0XR78_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylformylglycinamidine cyclo-ligase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0832269</name>
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    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
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    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="6">
        <location>
            <begin position="140"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="7">
        <location>
            <begin position="209"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="374" mass="39849" checksum="FCA30B97BD3E1A8B" modified="2024-05-29" version="1">MNTNFGASIDLARCNSTSLSLCSPNLEISVAKLHVPVLRYFSNRLSAYSFAPKESLCRRSRKASTRILYSISNTYVDESNKNEVGLTYKSAGVDIDAGSELVKRIAKMTPGIGGFGGLYPFGDSYLVAGTDGVGTKLKLAFETGIHDTIGIDLVIKGIVEGCQQSECVLLGGETAEMPDFYADGEYDLSGFAVGSVKKDSVINGKNIVDGDVLIGLPSSGIHSNGFSLVRRVLTRSGLSLKDNLPGESATLAEALMAPTVIYVKQVLDIISKGGVKGIAHITGGGFTDNIPRVFPEGLGAVIFKDSWVVPPIFKWIQKAGSVEDAEMRRTFNMGIGMVLVVSKEAALRLLEDAQATNVAYRIGEVVKGDGVSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRL4</accession>
    <name>A0AAF0XRL4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DCAR_0831478</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRL4"/>
    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099:SF42">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099">
        <property type="entry name" value="CYSTEINE-RICH REPEAT SECRETORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042197291" description="Gnk2-homologous domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="24"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="137"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH11982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="32873" checksum="42055B6C10FFB656" modified="2024-05-29" version="1">MVVLFMLCCLLIIVPCSVEAAPPEYMYHICRNSSTNTPNTIFQANRNVLLSTLASNATQNKGYYNYTAGRNSKDMAYGNSLCQGDLVPKDCQQCVTAATDDIKNKCPNGIFGIIWYAECMVRYDNKAFFTLFGVEEEGLAVRLWNTQNVTDEARFQQVLGNTMNNLVTKASEGKPLNDQTIKSFALGTAKLSSLQNLYALAQCVPDLPEAACSRCLRVAVNLLQYHSNSQGGRALVSTCTVRYELYPFYRNIATAPPPSQVTSPPPAPPHSEGPSSITGHKGSIYVYFYKLMLSSYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSQ0</accession>
    <name>A0AAF0XSQ0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0833103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSQ0"/>
    <dbReference type="KEGG" id="dcr:108197032"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001374">
        <property type="entry name" value="RNI-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
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        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="611" mass="65921" checksum="621119A594BD0227" modified="2024-05-29" version="1">MASTNTLSLHFTPKIVPLETRPRNALSRNFPCSSASYCGHKLLISRSLCINRRRRANLMFEKFAIRASASSRNRSSRRVHTESQSQATSPMGAAKEIASFIVPAGTFVIITFVLWKLVEMVLMPKPTKKVTEENKSSSSQGTKWSFAAGTNLMSGFGAKIERESKQTLNEFAKELRAFAVVDMSGRNFGDEGLFFLAESLAYNQTLEEVNLSANGITSNGLKAFDGVLQSNFTLKTLDLSGNPIGDEGAKYLSDILVNNNGIQKLRLNSTGVGDEGAKAIAEMLKANTSLRFLELNNNLIDYSGFSGLAGSLLENKTINAMYLNGNYGGALGAASLAKGLEGNKSLRELYVNGNSIGDEGVRALVSGLLLHKGKLTALDIGNNAISSKGAHHVAEFIKSSKSLTWISIYMNDIGDEGAASMADALRENRTITSIDLGGNDIHAKGIEGIARVLKDNTVISSLELGYNPMGPEGAKALSEVLKFHGNIKTLMLGWCQIGAKGAEYMADMLKYNNTIATLDLRANGLRDDGAICLARSLKVVNEALVSLDLGFNEIRDEAAFSISQALKANEDVRLTSLNLSGNFLTKLGQSAIADARDHVFEMTEKELNVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSV5</accession>
    <name>A0AAF0XSV5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0831688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribosome-inactivating protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSV5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.470.10">
        <property type="entry name" value="Ricin (A Subunit), domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017989">
        <property type="entry name" value="Ribosome_inactivat_1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016139">
        <property type="entry name" value="Ribosome_inactivat_prot_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453:SF34">
        <property type="entry name" value="RIBOSOME-INACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00396">
        <property type="entry name" value="SHIGARICIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0800">Toxin</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="4">
        <location>
            <begin position="265"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH12189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="450" mass="49527" checksum="86F9B242BCD01B08" modified="2024-05-29" version="1">MPFYTRGANQTTYADFIHNLRDEMGSQSTIYGIPQLQDAASLANEDRYVEVTLYSSTGTSITVAIDKVNAYVIGYQNGYDAYFFPGQDVPETSNVFVGATRCLLPFNSSYSALEKSAGTTRGSTKLGIGELDKAIQALYDKKEDSALACSMLIVIQMISDAVRSRYVEKLILQHISNDNSTSVFLPTPAMIAFQDEWKNLSSKITYSVGGVIYPPFILPTGSNQKVSVTSVFITFRLNIGVILFYCHPATAVPTPHSVTCQNIPEPTVLISGRNGYCADVESNFYRNGDAIILYPSEPDSIKWNINDNGNIENPKSGLAVSGFKGKTFSPLTVEKNTYASSQGWRLSNNTMPVVTPIIGFESLCMQACGGNTVEMLECSNTTAQKWKLYPDGTVRPYSATSNCLTSKSSQDVKTLVRAMEVMLNDGYSITIIAFLTQLKNLCWKLINRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSW2</accession>
    <name>A0AAF0XSW2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0833209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSW2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000020">
        <property type="entry name" value="ranBP2-type zinc finger protein At1g67325-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999:SF24">
        <property type="entry name" value="RANBP2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999">
        <property type="entry name" value="ZINC FINGER RAN-BINDING DOMAIN-CONTAINING PROTEIN 2 ZRANB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="342" mass="37768" checksum="78BF7E258579B13F" modified="2024-05-29" version="1">MPPVSQNHFVDNRNSSAAKRARTDGGRREDDWTCPTCGNVNFSFRTTCNMRNCTQPRPADHNSKFAPRPMQTLQGYSSSAPYGSAGAPSSMYIRVPPYGSSLFNQTGMPPYDAPFPGSSNYHYNYENRMSGGSPFRQLHLSGPTPYSSGAMVGNGGMYSVPPMMNRYGMGLPAGHTAMGPRPGFFSEDRLPDKNQEGTRGNDWTCPKCGNVNFSFRTVCNMRKCNTEKPGSQAVKSGKNSKPEMPEGSWKCEKCNNINYPFRTKCNRQNCGADKPSDSKKSPSEPVDDNDQFMWCLWILLSATFVSSQFVLVSPGFRHVTVGLCSLYFGRSSDLVGARNTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XXG1</accession>
    <name>A0AAF0XXG1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XXG1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF545">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18417" checksum="EE1899B705AB4E9C" modified="2024-05-29" version="1">MSVLNKAFELFRGLRKKDVVVYTAMIMGCGINGRDSDAIELFEEMIHSKLSPNLVTFTGLLTAFGHVGLVEEGYHCFNSMKNYGLVPSADHYSLMVDLLGQAGKEIAAKHCFELEPDTTSYRSLLANIYFSAGRWDDAKRLQTAIHDTAMEKGMAKISGSSWMVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y3G5</accession>
    <name>A0AAF0Y3G5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Sieve element occlusion C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0936164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="P:phloem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027944">
        <property type="entry name" value="SEO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039299">
        <property type="entry name" value="SEOA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232:SF20">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14577">
        <property type="entry name" value="SEO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sieve element occlusion C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH16606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="126" mass="14742" checksum="66FA320E34D58113" modified="2024-05-29" version="1">MWHSKVQHGKSVENDEIMKEIMMLLYFDGCDQGWAVIFWGEKDMAKAKGEMFLESLSEFDKWKHDSEEKGFLPALNDYFKKLYTPHHCHRFTFPGSTGSVEDKMVCAECGRPMEKSTMYTCCTNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJP9</accession>
    <name>A0AAF1AJP9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0104310</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJP9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="592"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG85123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="869" mass="96527" checksum="E503E773D882F254" modified="2024-05-29" version="1">MHIILILTFYTHICFNNKFEKCSKIMSVIVILRSQNLPGKLPPEFAKLPYLQVIDLSINYIEGTLPLQWASLRLRQISLRGNRLTGPIPEEYGSIATLEKLDLNANQMSGNIPPKLGDLPAISAISLSSNYFTGPLPNTLGRLTSLTDFRISDNNFTGRIPSYIQTWTKLKKLEIQASGLDGPIPDFSLLADLKDLRISDLNGNDGKFPSLNKSSFGEFQRLILRSCNIVGQLPEYLGEMQYLTTLDLSFNRLSGNISSTMSNLSSVKNIYFAGNLLSGSLPDWIFKSNNIDLSYNNLTLVSSEDDNCLKEGLNLFGSSSKSSPSGMVSCLQSQTCSRYVDSFYINCGGKQTSVDGNKYAADRQSSTEPSKFYRGENWASSSTGSFVEGYKYNFTRTSSSFNGDHRELLMDARLSPLSLTYYGFCLANGKYTVKLYFAEITFTDNHTFSSLGRRIFDIYIQGKLERKDFNIKDEAGGINKEVVVEFPVSVDNHTLEIRFYWAGKGTTIIPLQGIYGPLISAIAASHVKRNVSVGTVVGVMVGIASVLLLLLGILWWRGCLCRRNTMRSGLQGLDLHTGSFTLQQIRAATKNFDSANKIGEGGFGPVYKGVLLDGTVIAVKQLSHKSTQGTREFVTEIGMISALQHPNLVKLYGCCVERNELMLVYEYLENNSLFRALFERDRLELNWSTRYKVCIGIARGLAYLHEESRLKIVHRDIKATNVLLDKDLNPKISDFGLAKLAEEDKTHISTRIAGTFGYMAPEYAMRGYLTDKADVYSFGIVALEIVSGRSNSNSGPKQEPIYLLDWAYELEAEGKLMELVDESLGSEYEEEVLLMIHVGLLCTNATSAERPAMSSVVSILEGSVKKKCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AL78</accession>
    <name>A0AAF1AL78_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AL78"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG86803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19519" checksum="E814DA9C220C6CE0" modified="2024-05-29" version="1">MADMEVCTCVAVWIWTHKENVLNPNRKDLQSEKLPRNVPSTSSALFFKYTALGRPTDYRVLVDTNLGWFDVLLCPMTFCTYIELLKNGTPCITDYPRFERLPCTHKGTYADDCLVERVTQHKCYMVATCDRNLKRRICKIPGVPIMYITKHKYSIERLPEATMGGAPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANF0</accession>
    <name>A0AAF1ANF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208832</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANF0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042010435" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="79"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="542" mass="60318" checksum="5D2DC8E5A05A2814" modified="2024-05-29" version="1">MTTPVLLPMFSQLLLLFSLMSLSNAAETSDYTTFIECLVKNSSPDDQASVSSIVYSPQNSSFNSVLQAYIKNKRFNTSATPKPAIIVTATKESHVQNTVICAKNMGIQLKIRSGGHDYDGISYVSDTTFIILDLFNIRKIDVNIATETAVVEAGATLGELYYRIWEKSDVHGFPAGVCPTVGVGGHLSGGGYGNMLRKFGLSVDNVIDARIVDVNGKILDRKSMGEDLFWAIRGGGGASFGVILSYTIQLVKVPKITTVFRVMRSLDENATDIVHRYQFVVDKLDDGLFIRMLLQPVSGKVKGQKTIRATFIGMFLGDSSTLKGIMDKGFPELGLKKEDYSEMSWIRSVMWWANFDNTTSLDTLLSRNSDGVNFLKRKSDYVQTPIPKDGLESIWKKMVELGKVGFVLNPYGGKMSEISSSATPFPHRAGNIYKLQYSVNWHEEGAEADKNYMSQIRSLYSFMTPYVSSSPRGAFLNYRDLDIGVSDHGKDSYTEGQVYGEKYFGANFDRLVKVKSAVDPENFFRNEQSIPVKVMQSRRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYC4</accession>
    <name>A0AAF1AYC4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416238</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="uncharacterized protein LOC106759733 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="236"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="CW-type" evidence="7">
        <location>
            <begin position="370"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG96900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53418" checksum="EFE4F96945DF64A1" modified="2024-05-29" version="1">MSNNSSPTIYHRNKQGEQLRDSDTVFSFQTSTKANPTVSTSQHSNLGNMSKNSTPILYQRRKQIRDFDTDRPLQTSTKANPTNNSLSVISSEVPTLAANKTLVVDAEAAKNYEFLPFKNTIETASSRQSLGKEPGLVEDSRFNMIRDVDVCLANESCSSSKSNMDMCGASMKAKGDDTCECSSSEAFIQKSMRDDLSEKDIRILILKVLRSDGGPCRMRTHVSPEFPGSGNECSCLRQCKACDQSEFTLKMLICDQCEEAFHISCCYPRLRKLPLNEWLCYSCSKKKHKILKEMGRGRSAISKGTSGPIAAMLEDTEPYYTSVHIGLEYQAEVPDWSGPLTSEVHNFEEPLETSHSCSFQDGNSRKPSKISSICNWLQCQDIICGVGEGLDGTVCGKWRRAPLFEVQTDDWECFRCVLWDPAHADCAVPQEVDTSRVLKDLKFIQMLLKRKPGCSKNMDSNDPDEDVIWIPMTLTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B022</accession>
    <name>A0AAF1B022_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0520388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH01010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="14859" checksum="C6A20CD009BB5428" modified="2024-05-29" version="1">MHLTKSKEARTVRDWESVEEEIHLAISSGVDSSPQFLGATGCSDLLTTKAQVYMAAGRFEEAVAAAQCAAKLDPTEEAKATAERALALPSARSEGNQLFKASRFSDALKVYAEGLQHQALNSVLLCNRHQHTCQQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1F1</accession>
    <name>A0AAF1B1F1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">CGL160/ATPI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108223497"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056309">
        <property type="entry name" value="CGL160/ATPI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34118">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34118:SF6">
        <property type="entry name" value="PROTEIN CONSERVED ONLY IN THE GREEN LINEAGE 160, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24763">
        <property type="entry name" value="CGL160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="CGL160/ATPI" evidence="7">
        <location>
            <begin position="196"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH00156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="340" mass="37525" checksum="0DF37B2D969EF52B" modified="2024-05-29" version="1">MAASVYCCVSISKSLSAAEDLGSTTTSPISNTRPTKIILPKKKPEKWSTGTAPGEYGGPPLTTKLRKSWGGPGEDPITSDDYIWNRDFMPRMKNLFQDPSQNPLFDQPQEESSGFLSLNRVMSLDNMDADFSKELRAPSKILTETKVERAQTSGDLKQKWRPAPTRREQDKWDRAYKAATGGSEVMLREIKKPVGDPEVLAAQSREQYYKLKNKMQLLTLGIGGIGLISAYVSYTPEIAASYGVGFLGSLAYIRMLGNSVDSMADGARGIVKGAIGQPRLLVPVVLVMIFNRWNGILVPEYGIMQLELIPILVGFFTYKIATFTQAIEEALTIVQKKSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7P6</accession>
    <name>A0AAF1B7P6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0626336</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </alternativeName>
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            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Macko-Podgorni A."/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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            <begin position="9"/>
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        <source>
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            <dbReference type="EMBL" id="WOH16174.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="398" mass="44165" checksum="CF7F598890114BD2" modified="2024-05-29" version="1">MGKSDDSRQRRKNKLKTRKQNDANQVSTRVAAIIAAKKRRKTGKRTMCQGMCYSLPTPEDPFNDRYAKEETDKIKEKKQKPRKDDRGKVINSNKEAQKKTTNALHERKGKYLNVDEEKMIDDMQQDGDINVTKIRAHLAGKSGKNGQPFEILECPSKFLILCLNSIQNALRHECASFTDVDKPLFVDTWGVEFWKCFSLGKDMVDVSGACSTTEQIAWITSTAADTIARKEKEGLSIPIPFLLFLVASKEKASMVRSVCKPLKALGIHSVSLHAGASLDHQIQGLKSCEPEFLVSTPERLLELLSLNAVDIAGISLLVVDEGIQPEIIKSVRKHIHGTPQTLIFNNCLDNESSPAVDSLLPQTVCRLGVKDAVKASASCNLLQNFTTNTGYPIWLLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1BII9_DAUCS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041968004" evidence="2">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="51" mass="5624" checksum="74FC81C0C119370F" modified="2024-05-29" version="1">MEMTRTLLFFFVAVAYLSLTAAQDMDFAPAPTPVSIATLALVSLFVTLLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZQU5</accession>
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        <recommendedName>
            <fullName evidence="13">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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        <name evidence="14" type="ORF">DCAR_025925</name>
        <name evidence="15" type="ORF">DCAR_0729782</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KZM88850"/>
        <property type="gene ID" value="DCAR_025925"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196647"/>
    <dbReference type="OMA" id="VTCVLVY"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000025">
        <property type="entry name" value="probable polyol transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF453">
        <property type="entry name" value="POLYOL TRANSPORTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="13">
        <location>
            <begin position="24"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZM88850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH10315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="507" mass="55315" checksum="044353DB78CB761A" modified="2016-07-06" version="1">MEAEKGNKLTGDYTNKINKYAFACSVVASVISIIFGYDTGVMSGAMIFIEEDLKIDDMQIQVLAGILNICALVGALLAGRVSDYVGRRYTILLASIIFLLGSVLMGYGPNYPILLTGRCVAGIGVGFALMIAPVYSAEISSPSSRGFLTSLPELCISIGILSGYVSNYFFARLTLQVGWRIMLGLAAVPSLLLAFFILRLPESPRWLVMQGRLAEAKKILLLVSNSENEAQVRFSDIKLAAGIDENCTDDFVKVKNSNGQNIWKDLILRPSPPVRWMLLAAIGIHFFEHATGIEAVVLYGPRIFKKAGIHSKNKLLLAQVGTGITRIVFITIATLSLDKIGRRKLLLTSVGGMFFALVGLGLGLTIVEHSKEKLLWALSLCIGSTYTYVMFFSVGLAPITWVYSSEIFPLKLRAQGHSIGVAVNRLMNATISITFISIYKAITIGGAFFMFAGISVLALIFFFFFLPETRGRSLEEMEELFSKKSQPKIVGMGSSNQPTNNSCVQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZST7</accession>
    <name>A0A161ZST7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_021813</name>
        <name evidence="3" type="ORF">DCAR_0624393</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZST7"/>
    <dbReference type="Gramene" id="KZM90822">
        <property type="protein sequence ID" value="KZM90822"/>
        <property type="gene ID" value="DCAR_021813"/>
    </dbReference>
    <dbReference type="OMA" id="CLAITEF"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM90822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH04981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="125" mass="13897" checksum="B402D6C40832A9C3" modified="2016-07-06" version="1">MSGYQNSPPDPYRHYPPPGYGPPPGYPSAPPPPPSPYDAYPPQAPPGYPYAAPQTQGYQGYFNGGGYPPPPPQQLPPYQVYQYDEHHHHCHDHHDDGSSFLRGCLAITEFGDAFVFIVKFFLNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A4R3</accession>
    <name>A0A162A4R3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL17c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">50S ribosomal protein L17, chloroplastic</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">CL17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_018592</name>
        <name evidence="9" type="ORF">DCAR_0521274</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A4R3"/>
    <dbReference type="STRING" id="79200.A0A162A4R3"/>
    <dbReference type="Gramene" id="KZM95350">
        <property type="protein sequence ID" value="KZM95350"/>
        <property type="gene ID" value="DCAR_018592"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223254"/>
    <dbReference type="OMA" id="WQSFENG"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1030.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_bL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM95350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH01888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="204" mass="22881" checksum="D07137BFCB07784D" modified="2016-07-06" version="1">MASVSSLATTTATTWNMSCLKAALPPIYNTSARLPVVSSPCVKISLKPNPISLRSFHGLAPLHPLLSREGNSFDQCFTIIDNGGRISMMRHGRRVPKLNRPPDQRKALLRGLTTQLLKYGRIKTTRARASAMRKHVDKMITMAKDGSLHKRRQALGYIYEKQIVHALFAEVPERYGERNGGYTRIIRTLPRRGDNAPMAYIELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AAW8</accession>
    <name>A0A162AAW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair RAD52-like protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_014056</name>
        <name evidence="5" type="ORF">DCAR_0416894</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AAW8"/>
    <dbReference type="STRING" id="79200.A0A162AAW8"/>
    <dbReference type="Gramene" id="KZM98582">
        <property type="protein sequence ID" value="KZM98582"/>
        <property type="gene ID" value="DCAR_014056"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216583"/>
    <dbReference type="OMA" id="SKYIPWH"/>
    <dbReference type="OrthoDB" id="1935514at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037489">
        <property type="entry name" value="RAD52-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050:SF1">
        <property type="entry name" value="DNA REPAIR RAD52-LIKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050">
        <property type="entry name" value="DNA REPAIR RAD52-LIKE PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM98582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG97553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20471" checksum="4CCFC684F7E8A23C" modified="2016-07-06" version="1">MAFRFVMKSSAAVKTVSPLPFNHIICRTFAGKRVKEEKPEVELPDYNDVVPTSGISRPLSEILKEINKRVPDSLVKLRTESNGFSVKYIPWHIVNRILNLHAPEWSGEVRNITYSADGKSVSVVYRVTLYGTDAEIFRESTGTASLNEAGGYGDPVQKAEGMAFRRACARFGLGLHLYHEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AD97</accession>
    <name>A0A162AD97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF309 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_013508</name>
        <name evidence="3" type="ORF">DCAR_0417519</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AD97"/>
    <dbReference type="Gramene" id="KZM99130">
        <property type="protein sequence ID" value="KZM99130"/>
        <property type="gene ID" value="DCAR_013508"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218642"/>
    <dbReference type="OMA" id="DNEENWN"/>
    <dbReference type="OrthoDB" id="2020115at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831086" description="DUF309 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM99130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28836" checksum="86C24D0FC07824BD" modified="2016-07-06" version="1">MSITRPPWLLSSPLLFLAATFSSSRVTLSSCPPLNYLLSSLSTQNPVRQSHRTASYRYSSYGEDEEDDALSCSFDEAVLLFNERDYYKCHDVLESLWNKSQDPVRTLVHGILQCAVGFHHLFNQNHKGAMMELGEGVCKLRKMNLDSGPFHEFEQEISAVLEFIYQTQLEFAACIEDYCLAMDQSEQSYKLLGGYGARQRMYSLDLETDNKVAFIVFCPEGVESYSAVASSDRPRIKLPNLLATQQQLMDLDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ALY4</accession>
    <name>A0A162ALY4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103618</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ALY4"/>
    <dbReference type="Gramene" id="KZN10756">
        <property type="protein sequence ID" value="KZN10756"/>
        <property type="gene ID" value="DCAR_003412"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208939"/>
    <dbReference type="OMA" id="VGIAMDY"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000206">
        <property type="entry name" value="Universal stress protein family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF1">
        <property type="entry name" value="OS02G0773200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="167" mass="18034" checksum="810281A358AA25E6" modified="2016-07-06" version="1">MANVGCRRVGIAMDYSATSKTALRWAVNNLLGVGDEIIIIHVVSPKIDHSSNKQLFEDTGSPLVPLEELKEPNVLKKYGLTHDPEVLDILDTVSRTKGAKVLAKIYWGDPRDKLCEAVEHLKLGSLVIGSRGLGALKRVLLGSVSNYVVQNATCPVTVVKGAPVSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B438</accession>
    <name>A0A162B438_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_002409</name>
        <name evidence="8" type="ORF">DCAR_0102506</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B438"/>
    <dbReference type="STRING" id="79200.A0A162B438"/>
    <dbReference type="Gramene" id="KZN09753">
        <property type="protein sequence ID" value="KZN09753"/>
        <property type="gene ID" value="DCAR_002409"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225893"/>
    <dbReference type="OrthoDB" id="1904546at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05489">
        <property type="entry name" value="xylanase_inhibitor_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000045">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033868">
        <property type="entry name" value="Xylanase_inhibitor_I-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF103">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPTIDASE_A1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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        <location>
            <begin position="24"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN09753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG83331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="440" mass="47363" checksum="630E3D255F96A3A3" modified="2016-07-06" version="1">MAFPSSLLFSLLCLFLYTSSSLAKTKSGPKQLVLPIYKDASTLQYLTQIKQRTPLVPVHLTIDLGGRSLWTDCEHGYISTSIQPGGCGTTQCRLAQASKECEPCQPTFPVGPGCTNSPTCWRFPQNTVTRSASGGELASDIITINSTDGKSRGDVVIVPQFLFFCGLTTIVDGLAKGVTGMAGLGRTKISFPVQLASALKLKRKFALCLSSSATSNGVVIFGEGPYNFPTNSNDSTFLTYTSLILNKVNTEVRVLRGEASAEYFIGVKSIRINNKPVKLNTTLLQINSSGYGGTKISTVNPYTVLETSIYNAVIKAFVSELKNVTRVPAVAPFGACFSSKNIGSTRVGPAVPTIDLVMEESGVFWRMFGANSMVRVTKNVMCLGFVDGGEATFITSIVIGGHQVEENLLTFDLDKSRLGFSSSLLLRQITCENYRVLAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XIE1</accession>
    <name>A0A164XIE1_DAUCS</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XIE1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="21666" checksum="51F5D202C66A7F52" modified="2016-07-06" version="1">MRSLRFSPLTNTTNDTASANASVNASDQSLFGRKAPRTTALRKGLPVESGSSGKTAKRKVDFQTASKDNIAEENTTEKGYAHVYKDHSNDDSDADVVAKGACPTDELEEVVTVPKIKENWKVRSWHEFISDENDNGYYQRLYRNSEIYEDKEFGKIILKPWMIFLDKNHYKNTLNDYCIQKRCWQTLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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    <name>A0A164Y6Y8_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="1" type="ORF">DCAR_0519794</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Y6Y8"/>
    <dbReference type="Gramene" id="KZM94067">
        <property type="protein sequence ID" value="KZM94067"/>
        <property type="gene ID" value="DCAR_017312"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223212"/>
    <dbReference type="OMA" id="KLQVPPI"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_4_5"/>
    </dbReference>
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        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH00434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="470" mass="52617" checksum="FF125A52FF3DF646" modified="2016-07-06" version="1">MAKQQFSQYSFSSILHKRWLLRLFILLSLIAAFAFIIRAAFDSSTHCAQLQPDQRFHSAAPLGPALNPLGFIKSKLVLLVSHELSLSGGPLLLMELAFLLRGAGSEVCWITNQKPAGADQVTYALENKMLDRGVQVVAAKSQEAVKVALKADMVVLNTAVAGKWLDAVLKENVPRVLPKVLWWIHEMRGHYFSMDYVKHLPLVAGAMIDSHVTAEYWKNRTQERLRIKMPKTYVVHLGNSNELMEVAEDRVAKRILREHVRESLGVRNEDILFAHINSVSRGKGQDLFLRAFDESLRLIKEQKLQVPSIHAVIVGSDMSVQTKFETELRNFVSSNNIQHRVHFVNKTLTVAPYLAAVDVLVQNSQGRGECFGRITIEAMAFQLPVLGTAAGGTTEIVVNGSTGLLHPAGKEGVTPLAKNIVKLATHVERRLTMGKKGYERVKEMFLERHMSHRIASVLKEVLHHSKNQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z7A9</accession>
    <name>A0A164Z7A9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SelT-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_018696</name>
        <name evidence="5" type="ORF">DCAR_0521395</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z7A9"/>
    <dbReference type="STRING" id="79200.A0A164Z7A9"/>
    <dbReference type="Gramene" id="KZM95454">
        <property type="protein sequence ID" value="KZM95454"/>
        <property type="gene ID" value="DCAR_018696"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220160"/>
    <dbReference type="OMA" id="FQIIDFH"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019389">
        <property type="entry name" value="Selenoprotein_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544">
        <property type="entry name" value="SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544:SF0">
        <property type="entry name" value="THIOREDOXIN REDUCTASE-LIKE SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007854791" description="SelT-like protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM95454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25207" checksum="C7A543619E76BAEF" modified="2016-07-06" version="1">MDRMQLLLVGLPLFLFCSDIVNLFAPPPPKAPQHNHHHNHHKIQSSSPVIQETLQFPTQKVSGGIGGIGIGNTVTIDFCSSCSYKGNAVTMKNMLESSFPGIDVVLANYPPPLPKRLLSKMVPVVQFGMIGIVMGGEQIFPRLGFVAPPPWFYSLRANRFGTISSTWLLGNFLQSFLQNTGAFEVSINGEQVFSKLREHRFPGEIELKDLIGKKIGASRVVDSFGGAAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZT84</accession>
    <name>A0A164ZT84_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0522432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZT84"/>
    <dbReference type="Gramene" id="KZM96367">
        <property type="protein sequence ID" value="KZM96367"/>
        <property type="gene ID" value="DCAR_019609"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM96369">
        <property type="protein sequence ID" value="KZM96369"/>
        <property type="gene ID" value="DCAR_019611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF30">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="360" mass="41426" checksum="8352EA95DCF5A4A7" modified="2016-07-06" version="1">MSVHTVLFLLLLLLHQATSLHQEANEKKVYEQRKLYRSCNLFEGSWIYDDSYPLYDGSICPFINAGLNCQKNGRPDKLYLKYRWNPVGCSLARFDGEAFLERFRGKKIMFVGDSLSSNQWQSLTCMVQNAVPSSNYTLVQRKSFYSLTFPEFGVSIVYMKDGFLVDLEFRDIGKVLRLDSISINRSKQWKDTDILIFNSYHWWTHTGRLQTWDYFQVGDKVIKEMDHMEAYKIALSTWANWVDSSINPTKTQVFFQGITAVHYKGRDWNEPMANSCDGQTQPIPGSDYPGNRYPGEPIVKSTLARMAKPVILLDIALLTQLRKDGHPSRYADGGIDCSHWCVAGVPDAWNEILYTILVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A035</accession>
    <name>A0A165A035_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0414758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A035"/>
    <dbReference type="Gramene" id="KZM96697">
        <property type="protein sequence ID" value="KZM96697"/>
        <property type="gene ID" value="DCAR_015941"/>
    </dbReference>
    <dbReference type="OMA" id="ITRDWVK"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056362">
        <property type="entry name" value="AtuA-like_ferredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47472:SF1">
        <property type="entry name" value="DUF1446-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47472">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23544">
        <property type="entry name" value="AtuA_ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyclic terpene utilisation N-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="AtuA-like ferredoxin-fold" evidence="2">
        <location>
            <begin position="531"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="656" mass="71958" checksum="3CF18E769D074F66" modified="2016-07-06" version="1">MDVSHASPDSGTEEVEIHNCVVRKRVNPRKRKDKVYVGCGAGFGGDRPLAALRLLQRVEELNYLVLECLAERTLLDRYQAIKSGGVGYDPRIEEWMHLLLPLAVERGVCIITNMGANDACAAQKKVLETASTLGISITVGVAYQVEVSKAGVDHQMESFNDGVSVYLGAAPIVECLEKYRPNVIITSRVADAALFLAPMVYELGWNWDELQLLAQGSLAGHLLECGCQLTGGYFMHPGDRFRDIPFSDLLDLSLPFAEVGYDGEVCVAKADGSGGVLNFSTCAEQLLYEVGDPGAYVTPDVVIELRDVSFCSLTKDKVICSGAKPAAVLVPDKLLLLVPKDSGWKGWGEISYGGCECIKRARAAEFLVKSWLEEIYPGVSNHIISYIIGLDSLKTSHIDDRMALERSSSDIRLRMDGLFKQEDHAIQFTKEFMALYTNGPAGGGGISIGHKKESSLEKALVSREHVYWQIAAENISVNLNNQIPCLQEFKQDHAKKNSISISIQKETISRSSEQLSLSEILSSPALSGQPIPLYSVAHSRAGDKGNNLNFSIIPHFPPDIVRLKSILTPKWVKEVISTLVNPSSFPTTDDIERRDKWVNEHIAVEIYEVGGIKSLNVVVRDILDGGVNCSRRIDRHGKTISDLILAQVVVLPPESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A9G5</accession>
    <name>A0A165A9G5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_015492</name>
        <name evidence="13" type="ORF">DCAR_0415260</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="79200.A0A165A9G5"/>
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        <property type="gene ID" value="DCAR_015492"/>
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    <dbReference type="KEGG" id="dcr:108219358"/>
    <dbReference type="OMA" id="NHKGPNG"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="46"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="245"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM97146.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG95931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="545" mass="61899" checksum="262553D96B09A07C" modified="2016-07-06" version="1">MAEVDHNDDVQSETSDYTSEDEGTEDYRKGGYHAVQIGDTFKHARYVVQSKLGWGHFSTVWLSWDTHKSRYVALKIQKSAQHYTEAAMDEIKILKQIAEEDPDDKKSVVKLLDHFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDYRGIPLPKVKEICSHILGGLDYLHRKLSIIHTDLKPENVLLLSMIDPTKDPTKSGVPLIIPSKKDKIVPGSGACREVRSYNSDLTKNQKKKIRKKAKKAAQTCIEEEPSEESVPVNESSATEVSHQDEKSNNESNEEKSTRDRSTTTGSNRNSQQGNQGHRRGSRSARRKLLSEIDTRCKLVDFGNACWTYKQFTSDIQTRQYRCPEVLLGSKYSTSADLWSFACICFELATGDVLFDPHSGDNYDRDEDHLALMMELLGMMPRKIALGGRYSRDFFNRYGDLRHIRRLRFWPLNKVLVEKYEFSEQDAKDLADFLVPILDFVPEKRPTAAQCLSHPWFADGPKEADGPCTIEPTVVPPEAKDKIMSEKKRENDEREAMEVAVGNIAIDGASKPVNHPKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y1B9</accession>
    <name>A0A165Y1B9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_013874</name>
        <name evidence="3" type="ORF">DCAR_0417109</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
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    </dbReference>
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        <property type="gene ID" value="DCAR_013874"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="THAUMATIN-LIKE PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thaumatin"/>
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        <property type="entry name" value="THAUMATIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
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        </location>
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        </location>
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        <location>
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            <end position="212"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM98764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG97768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="30378" checksum="A17C5BACD5BFC9EF" modified="2016-07-06" version="1">MDRVLLKPCSTHVLYFILLVFYVANWSNVVSAATFTLINKCNYNVWPGILANAGTAKLDSTGFQLTPGDSRTFQAPPGWSGRFWGRTDCTFDANTGQGTCKTGDCGSNQMECNGAGANPPATLAEFTIGSDSGPSTQDFYDVSLVDGYNLAMIIEASGGSGACGSTGCITDLNRLCPNELKDGDGQACKSACEAFGSPEYCCSGEYGSPDTCKPSVYSQIFKSACPRSYSYAYDDATSTFTCTGADYIITFCPSSPRYVEISSYSYNLTSPENFLMYIRCILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZFV4</accession>
    <name>A0A165ZFV4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0309877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZFV4"/>
    <dbReference type="Gramene" id="KZM99987">
        <property type="protein sequence ID" value="KZM99987"/>
        <property type="gene ID" value="DCAR_008742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG90633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="8840" checksum="C988BF6E01445EA5" modified="2016-07-06" version="1">MKQITFTLTILSLILISTISVQAEVAAPPFPPKNFCYDVCDCQTLCYHRIPVCHHHRCECHEHPKYYPIPTCPGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AK10</accession>
    <name>A0A166AK10_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0311442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AK10"/>
    <dbReference type="Gramene" id="KZN01382">
        <property type="protein sequence ID" value="KZN01382"/>
        <property type="gene ID" value="DCAR_010136"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214845"/>
    <dbReference type="OMA" id="IMEHPYN"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="261" mass="27830" checksum="3D0F997C8BAF2754" modified="2016-07-06" version="1">MKNSSHGQSEPAPLMDMKEIGQWSFFRAIIAEFLATLLFLYVTIATVIGHKKQETSCDGVGLLGIAWAFGGMIFVLVYCTANISGGHINPAVTFGMLVARKVTLVRAVAYMIAQCLGAICGAGLVKAIMEHPYNMLGGGANSVAPGYSKGSALGAEIIGTFILVLTVFSATDEANTAEKPFISALLPLPIGFAVFMVHLATIPITGTSINPARSFGPAVIYNHHKVWSDHWIFWIGPFLGALAAALVHQFVFKKAHVPRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BWA6</accession>
    <name>A0A166BWA6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0313229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BWA6"/>
    <dbReference type="Gramene" id="KZN02962">
        <property type="protein sequence ID" value="KZN02962"/>
        <property type="gene ID" value="DCAR_011718"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210953"/>
    <dbReference type="OMA" id="SVYVPDH"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000046">
        <property type="entry name" value="Aspartic proteinase PCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000073">
        <property type="entry name" value="Aspartic proteinase PCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF49">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG93941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="431" mass="47693" checksum="5FABFCC7E9DAD6FA" modified="2016-07-06" version="1">MATSLHVLLFNIIFIFSISSSSCKTTPSSLLLPLKTKFIPSRSLLKPPNKLSFHHNVSLTVSLTVGSPPQQVTMVLDTGSELSFLHCKKTPNRPLTFNPLASASYTPIPCSSPTCRVRTRDFTSPVSCDPKKLCHATLSYADASSVEGNLAADTFRVGSLDQPRTVFGCMDTYSSSNPEDSKTTGLMGMNRGSLSFVSQMGFPKFSYCISGRDASGVLLFGEASFSWLKPLNYTPLVQMTTPLPYYNRVAYTVQLEGIKVAGRVLPLPKSVYVPDHTGAGQTMVDSGTQFTFLLGPVYTALKMEYIRQTRGVLRVYDDPNFVFQGAMDLCYRVETTRKILPILPTVSFMFRGVEMSVSGDRLLYRVPDMKIGNDDIHCFTFGNSELLGMEAYIIGHHHQQNLWMEFDLEKSRVGLAEVRCDLASQRLGLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CXZ6</accession>
    <name>A0A166CXZ6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_005317</name>
        <name evidence="3" type="ORF">DCAR_0205971</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166CXZ6"/>
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        <property type="gene ID" value="DCAR_005317"/>
    </dbReference>
    <dbReference type="OMA" id="QVEYDYN"/>
    <dbReference type="OrthoDB" id="1707227at2759"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="entry name" value="At5g59350-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34054">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34054:SF16">
        <property type="entry name" value="MEMBRANE LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN04480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="29877" checksum="7FAF66E25FE4926C" modified="2016-07-06" version="1">MGYLSHVGIGLSFLFGCLLLGLIAELYYVIYWKKRYTSMEIQDSSHPTNGTTELSYLFCWKSPISSRKQELPTSVKSGHDQDLQMLRIYNGEEGVESELMRLHNLSGPPRFLFTIKEETKEDLESEDEKSRGGRNISRKGSRNNSLAELLACVETPYLSPLASPVLKKTQSLEGYNHQGFNPFFESSTDAEINKLRLSPPPKLKFLKDAEMKLMNKLMEDAVRKCHEKDATFLTASESNSTASSQVLPLATSPPAFNIVAKKSNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
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        <alternativeName>
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        <name evidence="10" type="ORF">DCAR_0207144</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_006331"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208418"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
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    </dbReference>
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        </location>
    </feature>
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            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN05494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG87911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="28461" checksum="7D495A7FDE2C4794" modified="2016-07-06" version="1">MGKKIVKFPSMFKKRSSSDQWQWPSSGKHNVNYENGDELMFKTVNSVFLEPCIGDQTSGDKPSYGWFTNAEYSPSLSDTSEQVEDEDDQEMEDELEEIIRGVRSDRLFFEPCNSLLGSSTTTTTTTTGTAATSDTRGASDDVCYNDVEELPFKESIVLAMESEDPYEDFKGSMLEMVESHGLKDWECLEELLEWYLKMNGKMNHGVIVQAFVDLLVGLASDDSTSFSSAASSFSASSSSPLSTVSRRELCNHDHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DS54</accession>
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        <recommendedName>
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        </recommendedName>
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    <gene>
        <name evidence="4" type="ORF">DCAR_006447</name>
        <name evidence="5" type="ORF">DCAR_0207272</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DS54"/>
    <dbReference type="Gramene" id="KZN05610">
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        <property type="gene ID" value="DCAR_006447"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="235"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="549"/>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
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            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN05610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG88039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="855" mass="96086" checksum="2D6B708B740A725A" modified="2016-07-06" version="1">MTIKRRRPRFSPAVFRCRSLLPHHSKSQTFNLYTSYSSQVSSQNEKQASIITKTPDTKTKLSHIFSQPISRYELLSPAHVTKVLLTHRNDPGSALHYFKWAEKKFNMVISTDPYLVLLHILAGSEDYYRVIRNMLNRMVSDCLSGIGPNLVDQLIDCAKRFDLEVNYVVFNYLLNGFVRAGRFIDAIECFGRIVECEISLSVWCINFLMSRLVRNNLIEEAKDLYAGLVVRGTYDCATVDVIMRACMKEERFDEAEKYFRQAKGSGVKLDVSVYCGAIYAVCKVPDADYAYELLKEMKGLGWSPAEGTYTCVVGAYMNQKNMVAALKVRDEMVADGIPMNLIVATSLMKGYYLEGDLGKALGLFEEIERDGPAPDKYTYSVLIQGCCRHGNMHKAKELYNRMKLSGIEPDAYHVNYLIRGFLEAELWEEALVQFHDAVECGVYNVFMYNVLMSSLGKAGNMSEACNILDDMLSKGLVPNVVSYNSLILGYCRQGNMDSASNLFADMQEKGPKPNVITYSILIDGHFRKGGTEDAMKYFNQLLSQGLTPTNYTYNILLNGLCKARRTSEARDRLKKLLEDGFIPDCMVYNSIIYGFVKENAINSALAVYTEMCESGICPNVITYTTLIDGLLKSNNYDLAIKLKNDMRNKGLKLDITAYGVFIDGYCKRRDMESARELCDELYAVGLSPNTVVYNSMISGFKSLSDMDTALMLYNKMIQDGIQCDLATYTTLIDGLLKVGKLLEATNLYTRMLADGIKPDVIVYSVLVSGLCNKGQVENARKIVKEMESKNAVPNVLIYNSIIAGYFREGNFVEGFKLHDEMIEKGVTPDDTTYDILVSRKASGNSSPISATFFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FJ87</accession>
    <name>A0A166FJ87_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_000508</name>
        <name evidence="2" type="ORF">DCAR_0100274</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN07839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FJ87"/>
    <dbReference type="Gramene" id="KZN07839">
        <property type="protein sequence ID" value="KZN07839"/>
        <property type="gene ID" value="DCAR_000508"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN07839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="8946" checksum="4A6300F81A092688" modified="2016-07-06" version="1">MDCFQFKLQLASEILHPSLDNYIKKKLKSRVKMFLRRSCTAKMFLRRCYSTFSSRPLRDCVVASGPAGFYTAKKVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FV02</accession>
    <name>A0A166FV02_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_001276</name>
        <name evidence="5" type="ORF">DCAR_0100702</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FV02"/>
    <dbReference type="STRING" id="79200.A0A166FV02"/>
    <dbReference type="Gramene" id="KZN08211">
        <property type="protein sequence ID" value="KZN08211"/>
        <property type="gene ID" value="DCAR_001276"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216567"/>
    <dbReference type="OMA" id="SMEANIA"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF236">
        <property type="entry name" value="OS02G0749800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN08211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG81551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="340" mass="39549" checksum="A422A020A25219EC" modified="2016-07-06" version="1">MYSYIYKNLIKNIANVSLKTAPLCFLNQRLFSDTVKNTSTVSLFKNYGFTEPQITTLLSKRPSLLSLSEDKNIRPRLDVFASYGFSSADIYQILVSDIDILRRGIKNQIVPCCEFLKSVVKDNESFIATVKRSTWVLKHDYKHNLQPNIEVLRDYGVPEYRIVRFLQVQPRVLMLGSDRFKVVVGEVLEMGFEPVKGHFLTAINALVGLSKENRERKWDLFKKWGWTDDEILLAFRKQPNVLVVSEEKLERVFGFLIKKMGWNAADVWSCPIVILHSLENWTMPRCLVVQFLLSKGVLPKDTTLNNFIVPMENRFKNKYVDKYCLKYPQVLKLYKRTVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G8L3</accession>
    <name>A0A166G8L3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="9">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0101256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:26210"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19626"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="9"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166G8L3"/>
    <dbReference type="Gramene" id="KZN08672">
        <property type="protein sequence ID" value="KZN08672"/>
        <property type="gene ID" value="DCAR_001202"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204936"/>
    <dbReference type="OMA" id="VQTNIVI"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000041">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000063">
        <property type="entry name" value="probable low-specificity L-threonine aldolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="10">
        <location>
            <begin position="8"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG82095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="361" mass="38646" checksum="8856D089F31417D0" modified="2016-07-06" version="1">MVVTRWVDLRSDTVTKPTEAMRAAMAKAEVDDDVLGFDPTALKLEKEMAKITGKEAALFVPSGTMGNLISVLTHCDVRGSEVILGHNSHIHIYENGGISTIGGVHPRTVLNNKDGTMDINLIEAAIRDPAFEICYPTTKLICLENSHCNAGGRCISVDYIDKVGELAKKHGLKLHIDGARIFNASTALGIPVDRLVQAADSVSICLSKGLGAPVGSVIVGSKSFIAKAKILRKTLGGGMRQIGVLCAAALVALQDNVGKLEDDHKKAKTLAEGLDRINGLKLDVAAVETNIVYLDILESSNLNAPKLCKALERHGILVMEDKPYRVRFALHHQISAADVQFTLSCIQQVVNEASDENGASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GQE5</accession>
    <name>A0A166GQE5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_001883</name>
        <name evidence="8" type="ORF">DCAR_0101894</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GQE5"/>
    <dbReference type="Gramene" id="KZN09227">
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        <property type="gene ID" value="DCAR_001883"/>
    </dbReference>
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    <dbReference type="OMA" id="MSANCVD"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
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        <property type="entry name" value="HYDROPHOBIC PROTEIN LTI6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN09227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG82726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="6271" checksum="A223D173C8CBB7A1" modified="2016-07-06" version="1">MKEGTATCVEIILAIILPPLGVFLRFGCQVEFWICLLLTFFGYLPGILYAVYAITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H378</accession>
    <name>A0A166H378_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0102406</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>phosphate(in) + H(+)(in) = phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:29941"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H378"/>
    <dbReference type="Gramene" id="KZN09664">
        <property type="protein sequence ID" value="KZN09664"/>
        <property type="gene ID" value="DCAR_002320"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196151"/>
    <dbReference type="OMA" id="GLYHVRA"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000175">
        <property type="entry name" value="Inorganic phosphate transporter 1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="480"/>
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        <location>
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        </location>
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        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="520"/>
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        </location>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG83231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="531" mass="58891" checksum="9B6A194FBC12CF9F" modified="2016-07-06" version="1">MAREQLQVLNALDVAKTQWYHFTAIVIAGMGFFTDAYDLFCISLVTKLLGRIYYHVDDYPKPGTLPPGVAAAVNGVAFVGTIAGQLFFGWLGDKMGRKRVYGMTLMIMVICSIGSGLSFSKNPKAVMATLCFFRFWLGFGIGGDYPLSATIMSEYANKKTRGAFIASVFAMQGFGILAGGVFAIIVSASFKASFPAPAYEDNALGSTVDQADYVWRIILMFGAIPAAMTYYWRMKMPETARYTALVAKNAQQAASDMAKVLHVEIEVEQDKVEKTKDFGLFSREFLRRHGVHLLGTTSTWFLLDIAFYSSNLFQKDIFTAVGWLPHAETMNAIEEVYKIARAQTLIALCSTVPGYWFTVAFIDRIGRFKIQLMGFFFMTVFMFALAIPYDHWTHRKNRIGFVVMYSLTFFFSNFGPNATTFVVPAEIFPARLRSTCHGISAACGKAGAMVGAFGFLYAAQNQDKSKTDKGYPPGIGVKNSLIVLGVVNFLGMMFTFLVPEANGKSLEEMSQENVEEEEMESRPEDSRAVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I1M2</accession>
    <name>A0A166I1M2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0103506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
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    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the histone H2A family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG84323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_003306"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000009">
        <property type="entry name" value="Histone H2A"/>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="H2A/H2B/H3"/>
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        <property type="entry name" value="Histone-fold"/>
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        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
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    </dbReference>
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        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00125">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="10">
        <location>
            <begin position="98"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG84323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="14809" checksum="DBB15156FDBE98C4" modified="2016-07-06" version="1">MGNKEGKSTGGRGKTAPATKSVSRSSKAGLQFPVGRIARFLKAGKYSQRVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKTRVIPRHIQLAVRNDEELSKLLGSVTIANGGVMPNIHQNLLPKKVGKGKGDIGSASQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IB06</accession>
    <name>A0A166IB06_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the costars family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IB06"/>
    <dbReference type="Gramene" id="KZN10944">
        <property type="protein sequence ID" value="KZN10944"/>
        <property type="gene ID" value="DCAR_003600"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204313"/>
    <dbReference type="OMA" id="QRVHDNV"/>
    <dbReference type="OrthoDB" id="9871914at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032970">
        <property type="term" value="P:regulation of actin filament-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1540:FF:000002">
        <property type="entry name" value="costars family protein ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1540">
        <property type="entry name" value="Costar domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044302">
        <property type="entry name" value="Costars"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027817">
        <property type="entry name" value="Costars_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038095">
        <property type="entry name" value="Costars_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334:SF1">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14705">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01283">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9761" checksum="0D039F311ED89DA5" modified="2016-07-06" version="1">MNVEEEIDRLREEIQRLGKIQADGSYKVTFGVLFNDDRCANIFEALVGTLRAAKRRKVLTYDGELLLQGVHDNVEIILKPTTTVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J7J9</accession>
    <name>A0A166J7J9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0104908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
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        <source>
            <tissue evidence="1">Leaf</tissue>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH16323.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="15">Leaf</tissue>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <comment type="function">
        <text evidence="1">Confers resistance to late blight (Phytophthora infestans) races carrying the avirulence gene Avr1. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="1478287at2759"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
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        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1152">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="12">
        <location>
            <begin position="55"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="13">
        <location>
            <begin position="304"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="462"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022667"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM83865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH11133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="754" mass="85975" checksum="91DCF87AF5468A9E" modified="2016-09-07" version="1">MDCHSSKLIDDGSANQGELGEEYSSQDFPVQIYSRKNKFQEGEVFVGFQKEESDLLNQLASITKKKLQVISIVGMAGLGKTTLATRLYNHSYVESYFYARAWITCSQLYLKRESLLEILRSVSENTDEICAMNDNMLAHTLYRALKGRRYLIVVDDIWSIDDWNDIRRCFPDDKNGSRIMVTTRLKEVALHAQSDGNPLCLRFLTEEEGFDLFKRKLFEDGNLFGILGFLGKRITRNCCGLPLAIVLIAGVLKNNFDFLLWLQIQEHLSSYIISDYNQYMDTLALSYNHLPQHLKPCFLSFGAFPVGYDIPVRQLMWLWIAEGFIGQDGRERNLEDIAEDCLMDLINRSLVVVGKKRSNGAIKTCRIHDLLRDLCLRKAEEENFSPDIYKHNKVSFSCPHSLVHPKFQLLLSTNVLAIPSNCSCYSSEGSQSFCKQILSVWDASEHTRALDLSSIEFLVFPSEVLQLVHLRYLGLRFKSGNPPESISCLQELQTLIMSSRMNMVIPNDMWKMVNLKHLFIKSGENLVDISNVEGEQRTLDNLQTMSLVSPTRPCHNILARTPNLQKLGLCGPLTTTRGDFKCPDLGLLMQLEKLKLSNTISFHQGVRLSISTIFPGSLKNLTLSNTCLNWEEAWIFEMIPNLEVLKLKFHAFVGKDWETRPQAFPCLKFLKLKELDLVTWTVCRYDFPVLQHLQVYHCPYLTEIPEDLGNICTLESLEISGCNDAATNSASDLQEEQERYGNDSLQLIVNPSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3I6</accession>
    <name>A0AAF0W3I6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786">
        <property type="entry name" value="DNA BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786:SF66">
        <property type="entry name" value="HOOK MOTIF PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="25166" checksum="12B0055F2AE36332" modified="2024-05-29" version="1">MHAKVKLPLLKDPPLYLKTLLQNKDSKGRHFVKHIRMFNMMFSFTSMGGKVDFSINRGGAPWVFRLYGQNYHRIGTLCPQPGSPPKFLQLYIHDTDNEVSNRISAVSSGNDSDSFDAKIVNNLRRMLDEHNPLTQSFRNARDMFDSTYCPNLMIRLIGRRDYDGRTYNLPTASEVAALIVGDIKGNSDKRDIVVQTRVAILNGPKSKELWKSTACNQKTGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6F7</accession>
    <name>A0AAF0W6F7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0101713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108204264"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000013">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054506">
        <property type="entry name" value="DnaA_N-like_STI"/>
    </dbReference>
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        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF63">
        <property type="entry name" value="PROTEIN STICHEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23007">
        <property type="entry name" value="DnaA_N-like_STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22608">
        <property type="entry name" value="DNAX_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="526"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="125"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="846"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="885"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG82548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1276" mass="141171" checksum="5AE8E0DD03EF7FD6" modified="2024-05-29" version="1">MSKMSGGVIGNGRNGGFDPSNLHLKKELTQIRKASKTLKDPGTTSTWRSPLSSGRSLSVVANTSSLSTGNNHYYHHYRNNSNGNVINGDDNNFEVPLEAGTSNGSSGKGKEKGIFLYNWKAQKSEHEDSIESRNKKVRHRGDSEVTADDDETEDGDGLSSLLGESVDNSMSDARNGGGDSKSDTYIGDKYASILHKYRNTNLTPSGKRNSVKKKVKNAHSAALWKRQRQQNVLSRSSKPGSGPVPLNLRREDSLDLVDQSDDTGEYFNSEDLRRYSAESPLLARLKSSKLLRGSRKEDSVYSYSTPAMSTRSLNLYGIRNPSTVGSWDGTTASFNDGDDELDDHFDSAGQQGCGIPCYWSRRSTPKQRGTCRSCYSPSLSDTLRRKGNNILRGSQSMYSRRRHGFSLSSSKKRGSRTAQGLVPLLSSSGEGMGGSSLGNSNDELSTNYGELDLEALSRLDGRRWSASCRSEEGLELVALNGERDSESTPENLRSLSQKYRPVFFEELIGQNIVVQSLMNAIPRGRIAPIYIFQGPRGTGKTSTARIFSAALNCLAKDETKPCGVCRSCADFVSGKSKNLIEVDSSNKKGIGKVRYLLKTLFAGPPSTFSLYKIFVIDECHLLPAKTWLTFLKFLEEPPPRVVLIFITTDIDNVPRAVLSRSQKYIFNKIKDGDIVSRLRKIAAEEDLDVETDALDLIAVNADGSLRDAETMLDQLTLLGRRITTSLVNELVGVVSDEKLLELLELAMSSDTAETVKRAREMMDSGIDPMLLMSQLAALIMDIIAGTYHIVDAKGIDSFIGGRSLNEAELERLKNALKLLSEAEKHLRVSSERSTWFTATLLQLGSVPSPEPTQSVSSRRQSSRTTDEDPSSTSKEVITQKLKPDSQYTSRRSTSPMSLHNKATHRNSASQDDPLDLNSKPAYSQYLNGNSLSTSHGKFGAETTKSNMLDDIWIRCIERCHSKTLRQLLHNYGKLVSISEVEGAFVAYIAFTNSNIKLRAERFLSSITNSLEIVLRRNVEVRIVLLPEDDPCINSERQVALVDPMVKQHEAQVPLNLPRGSFNDPDVMLVRKLHSNSESASLQVENVELNGPKDREPEIPAKRIESIIHEQRLETAWLQTAEKGTPGTLNRSKPERNQVLPQDGVYHQNQMDFTDPASLTSQHWEDELKDEISALKIYNGKDATQKGQVGKKSDHYPMSPSLLHNSSYASNFSKEAMGYESSSGAGGCSGLFCWNNHKHHMKGKIKQRAPVRKHKGGRFLCLGECAKSSKSDENIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6P9</accession>
    <name>A0AAF0W6P9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Golgi apparatus membrane protein TVP15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0101864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6P9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34965">
        <property type="entry name" value="OS07G0118300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34965:SF1">
        <property type="entry name" value="OS07G0118300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG82698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22587" checksum="892A45914670A0CD" modified="2024-05-29" version="1">MSSAEGESSQSQQPTNGPRVRTRPDPFLITCRCFSFITCLASILCIIVNVLSAVRSFKNNSDLFDGIFRCYAVVIAIIVVVAETEWEVVMRFSKVLEYWVGRGMLQIFVAVMTRAFPEISTNRKDLLLLQNVASYMLLACGVVYVVSGLLCIGHLKRARQKKEVSAEQAIKDLEQAHRRDLELRVLNNRFTHNVTQMSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6X0</accession>
    <name>A0AAF0W6X0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0103343</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6X0"/>
    <dbReference type="KEGG" id="dcr:108201960"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041911044" description="Subtilisin-like protease" evidence="12">
        <location>
            <begin position="27"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="15">
        <location>
            <begin position="33"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="127"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="376"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="16">
        <location>
            <begin position="662"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG84162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="81712" checksum="147A1C0C07E8CB55" modified="2024-05-29" version="1">MASFSTTSSLLHHLILVLILVPLAFSDQTHKSYIFRVDYDAKPSIFPTHFHWYTSEFTDPTRILHTYDTVFHGFSASLTPSQAARTLQHPSVLASFEDRRRQLHTTRSPQFLGLRNQRGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNIGPVPKNWKGICQVGVKFSAKNCNKKIIGARFFSKGHEANEGFGGLGGGINDTIEYKSPRDADGHGTHTASTAAGRYAFKASFEGYAAGIAKGVAPKARLAVYKVCWKSSGCFDSDILAAFDAAVNDGVNVISISIGGGDGISSPYYLDPIAIGSFGAVSRGVFVSSSAGNDGPNGMSVTNVAPWIMTVGAGTIDRNFPADVILGDGRKISGVSLYSGLPITGKMYPLVYPGKSGVLSASLCMENSLDPSEVKGKIVICDRGNNPRVAKGLVVKKAGGIGMILANGISNGEGLVGDAHLLPACAVGSDEGDFIKGYTSAAVLPTASISFGGTIIGIKPAPVVASFSGRGPSGMNPEILKPDIIAPGVNILASWTDVVGPTGLDSDTRKTEFNILSGTSMSCPHVSGAAALLKSAHPDWSAAAIRSAMMTTANILNNMLHPMTDEATGKASTPYDFGAGHLNLDRAMDPGLVYDVVNSDYVSFLCAIGYGPKTIQVITRSPVNCPMKKPMAENLNYPSIAASFSSSSMGVASKTLMRTVTNVGDANAVYKVKVDAPKGTTVVVKPGKLVFTERIRKLNYFVTVKVVRKNIVMGDAGALFGSLSWMDGKHVVRSPIVVTQIDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W768</accession>
    <name>A0AAF0W768_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0102052</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="11477" checksum="7F479503192C6056" modified="2024-05-29" version="1">MWYLGPDNMGQVKPKYLLVPNPSIREALCLQEIIEIERARERRMSFLTPFKRANQYLRTHRTVDRTLSLMAIVAIGSAFWISSGPDKKPDKDEAVRTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9A8</accession>
    <name>A0AAF0W9A8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0104445</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9A8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF925">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG85257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="310" mass="34922" checksum="93C62601987B9B22" modified="2024-05-29" version="1">MLYNASRGVVWRTSFYVAVKRLSQNSGQGLEELRNETILIAKLQHRNLVRLLGCCIEKDEKILVYEYMPNKSLDSILFDPKKKALLNWDTRVQIIEGIAQGLLYLHQYSRIRIIHRDLKASNILLDAEMNPKISDFGMARIFGGNEFQANTTRIVGTYGYMSPEYAMEGRFSVKSDVFAFGVLLLEILSGRKNNSFLLSADYTNLLGYVWNLCERGKMLELIDPTLQVPSSSSKPLRYIIIGLLCVQESPDDRPSMSDVVSMFNNKHMELESPRRPAFAAGRTALSESSGTATSEIGSVNNLTTSVMEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAQ1</accession>
    <name>A0AAF0WAQ1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">APO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0103734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAQ1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023342">
        <property type="entry name" value="APO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05634">
        <property type="entry name" value="APO_RNA-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51499">
        <property type="entry name" value="APO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APO" evidence="1">
        <location>
            <begin position="48"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="APO" evidence="1">
        <location>
            <begin position="220"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG84550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="36614" checksum="EE28FFB45EB189F1" modified="2024-05-29" version="1">MGVEKPLLPPKNGLLVPKLVPVAHDVLDAWKIVIQGVSQLLHVIPVHACSECSEVYVGQAAHYIQDCQGATSAKRRNFHSWVKGSINDVLIPIDSYHLFDPFGPRVIHETRFAYDRIPALVELCIQAGVDIPGYPSRRRTVPIRMLGKKVIERGGSVEPPEQQCSPDAASMLEFDTYRTHERFSSPLELEVPGIAQKTIEAYERVKIGLKKLMKKYTVKACGYCSEVHVGPWGHNAKLCGEFKHQWRDGKHGWQDAIVDEVLPPNYVWHVEDPKGPPLRTALKRFYGKAPAVVELCLQAGAQIPAKYKPMMRVDIVVPDKEETHLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAY2</accession>
    <name>A0AAF0WAY2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0103869</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHI protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAY2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007818">
        <property type="entry name" value="SHI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006511">
        <property type="entry name" value="SHI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006510">
        <property type="entry name" value="Znf_LRP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01624">
        <property type="entry name" value="LRP1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01623">
        <property type="entry name" value="put_zinc_LRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604">
        <property type="entry name" value="PROTEIN LATERAL ROOT PRIMORDIUM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604:SF54">
        <property type="entry name" value="PROTEIN SHI RELATED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05142">
        <property type="entry name" value="DUF702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG84685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="193" mass="21912" checksum="D265C54529BA40BC" modified="2024-05-29" version="1">MKRCEECGNQAKKDCTYMRCRSCCRNRGFECQTHVKSTWVPVSTRRPRPLLHPLISTCTSATAHHHPFQNPKRLRHHHASSSSHHPLGTSSHNIYSLRLIQLKSVRRGDFPAEVSIPATFRCVRVSCDENAVDEYAYQTSVNIQGHVFKGILYDQGPAAQETSTSSFQHPNLFTTTSTYPFNVGTQFFPYPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBM3</accession>
    <name>A0AAF0WBM3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0104366</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBM3"/>
    <dbReference type="KEGG" id="dcr:108207486"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000225">
        <property type="entry name" value="cold-responsive protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG85178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="320" mass="35627" checksum="651ED9EED8B0DF3F" modified="2024-05-29" version="1">MRWGCCGSEETDDINVAGNVKKFSYKQLSAATHNFHPSNKIGRGGFGIVYKGVLKNGTRVAVKTLSAESKQGLREFLTEIDIISNVRHANLVKLIGCCVQGTNRILVYEYLENNSLDTALLGSDRKTIRLNWERRSAICKGTARGLAYLHEDLEPPIVHRDIKASNILLDKNFKPKIGDFGLAKLFPDNITHISTKIRGTTGYLAPEYMLGGKLTKKADVFSFGVLILEIVSGRSSGKPSWGGIQKLLLEWVRQLYEEGQLLEMVDPDLEGFPENEVIRCIKIALFCTQATASRRPTMSQVVDMLSRNTQLNEKELKVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCA1</accession>
    <name>A0AAF0WCA1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0205999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCA1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF18">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="9232" checksum="491746CF484AC338" modified="2024-05-29" version="1">MLVQHYLGNRLCKETDFMEKMKTYIWSSNLEIDKFESGWKAVIEEFKLEDNEWLSYMYEIKKSWIPSYFRDSPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WD23</accession>
    <name>A0AAF0WD23_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0105222</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Eukaryotic translation initiation factor 2 eIF2 is a heterotrimeric complex composed of an alpha, a beta and a gamma subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG86028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WD23"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="8">
        <location>
            <begin position="134"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG86028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="29895" checksum="5609A757D8D7DA8A" modified="2024-05-29" version="1">MGSDNPSSCLIAPFDPTKKKKKKKVVIQDPTDEPVDQIVESTENLTATDGTDTAFAGLKKKKKKQVQTDLLDDEKENMADDLDDIIGEDEDGEGIILQQQLPWEGTERDYEYEELLGRVFNILREHNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCRTMHRQPEHVMTFLLAELGTSGSLDGQQRLVVKGRFAPKNFEGILRRYINEYVICNGCKSPDTILSKENRLFFLRCEKCGSGRSVAQIKAGFQARVGRRKAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLF7</accession>
    <name>A0AAF0WLF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0310502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLF7"/>
    <dbReference type="SMR" id="A0AAF0WLF7"/>
    <dbReference type="KEGG" id="dcr:108214855"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG91254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="457" mass="51206" checksum="A1BF346A29F15F43" modified="2024-05-29" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN73</accession>
    <name>A0AAF0WN73_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0311703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as a component of a SCF E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the SCF-type E3 ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN73"/>
    <dbReference type="KEGG" id="dcr:108197295"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22151">
        <property type="entry name" value="F-box_AtGID2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000045">
        <property type="entry name" value="F-box protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG92436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="37838" checksum="B465800C4360CB01" modified="2024-05-29" version="1">MTSDYGLDVSAELESASQLRAAHFFSTQRPWLDLYGVYIKPVVPYGSAISKSSIDPALIHRYLPEELLFEIFTRMSPYTLGRAACVCRKWKYTIRNPVYWRNACLKAWQLYGVVENYKLLQSKYDGSWRKMWLLRPRIRTDGLYVSRNTYIRAGVAEWKVTNPVHVVCYFRYMRFYPSGRFLYKNSSQKVKDVAKFMNFRSAKVEGVYSGSYTLSEDKVEAAVLYPGLRPTVWRIRLRLRGTIVGANNRMDLLSLVTSGVNDTDMIGLDENILEIIEGWEEDETHNPDVPAISHKRGLTPFVFIPFEEVETSVLNLPVERMDYFVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNM5</accession>
    <name>A0AAF0WNM5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0310533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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    </reference>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            </authorList>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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            <tissue evidence="14">Leaf</tissue>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
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    <sequence length="244" mass="27959" checksum="21B4906306B0A825" modified="2024-05-29" version="1">MEATEEMSPRWLRPMLQASYFVPCLSHGVSTRIECNMFCLDCLGDAFCSHCLVHHKDHQVLQIRRSSYHNVVRVIEIQKLIDISCVQTYIINSARVVFLNARPQARLGKGVTNTCEVCCRSLLDNFRFCSLGCKLGGIERGDPRLTFTPRLKHDWEEMHGYESDEFSGPSKMRNERDSDQFMEARPYSADFRRVYGGGYEYGNAQEHNVPCSSSGTPHTSNHQDPVEPPRKRHRRKGIPHRAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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        </citation>
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            <tissue evidence="10">Leaf</tissue>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG93873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="281" mass="31930" checksum="A4593764B233B2CC" modified="2024-05-29" version="1">MGRQPCCDKVGLKKGPWTVDEDRKLVNFMLTNGQYSCWRAVPKLAGTISLCGKSCRLRWTNYLRPDLKRGMLSEHEENVVIDLHSQLGNRWSKIASHLPGRTDNEIKNHWNTHIKKKLRKRGIDPLTHRPLTPPIENFQEPENHNSSDVHQKRQTEKFIDPLTVLQDEMQRILEAPTLEVINNGFCADEVPLIEPHEILISNSSAPCCSSSSSTTTSNTNVNILLENSELLPTNSDWLNGDDSATNNVGFWDMDDGFSDLDFLFDDISCSRFLPLLEFGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT18</accession>
    <name>A0AAF0WT18_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0313253</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WT18"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="9">
        <location>
            <begin position="251"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG93963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="347" mass="38145" checksum="6BAE3CE3D27646A1" modified="2024-05-29" version="1">MKALILVGGFGTRLRPLTLSVPKPLVEFANKPMILHQIEALKAIGVTEVVLAINYQPELMMNFLKEFETKLDIRITISRETEPLGTAGPLALAREKLVDGSGEPFFVLNSDVISEYPLQQMIAFHKSHGGEASIMVTKVDEPSKYGVVVMEEASGQVERFVEKPKLFVGNKINAGIYLLNPSVLDRIELRPTSIEKEVFPQIAGEKKLFAMVLPGFWMDVGQPKDYITGLSLYLDSLRKRNSFKLATGPHIIGNVLVDESARIGEGCLIGPDVAIGPGCVVESGVRLSRCTVMRGVHLKKHACISSSIIGWHSTKHVHVCDEIYSNGGIVLPHKEIKSSILQPQILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVH9</accession>
    <name>A0AAF0WVH9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVH9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="20199" checksum="8E6955F43A340FF0" modified="2024-05-29" version="1">MEQRGAKRKHIEIVRKDLSVALTKLVQDSAKLNNDMRAAKEDFELMSKSMLNRKAEFLTQGLFLEEQHRRVIDILDGKNHGDEPEPIEHSTLLPPSSTTDMKKFSRESGEALRDHRIIREVEIQSLEGIFTQLDLMMKQNMKELRERAEGIERERRELSGKLLKLSRSTGDASGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWQ4</accession>
    <name>A0AAF0WWQ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Root cap</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0415197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WWQ4"/>
    <dbReference type="KEGG" id="dcr:108217101"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="entry name" value="Root_cap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31656">
        <property type="entry name" value="ROOT CAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06830">
        <property type="entry name" value="Root_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042137115" description="Root cap" evidence="2">
        <location>
            <begin position="27"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="52012" checksum="E21F5FC2805BA2B2" modified="2024-05-29" version="1">MDKRRAYILAAILVVFVSMEVAIVVGQGNGNNNGNGNVGSGNGNGNGNGNKNGNGSVGSGNGNNNGNGNTGGGNGNGNGNGNGNNNVNTGGNGNGNGNGNGNGNGNGNGNGNGGGNGNGNGNGNNNSGSGNGNGNGNGNSNNGSGNGNGNGNGNGNGNGNVNSNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGQVADANKYDMKAPVAGTGQQRALCKTRGACYNTTLTCPSECPERKPKKNKKQKGCFINCNSKCEAVCKYRKPKCDGYGSLCYDPRFVGGDGVMFYFHGAKGSDFAIVSDTNLQINAHFIGTRPSGRTRDYTWVQALSIMFDSHTLVLAAKRVTHWNDNVESLTVTWNSQTLTIPTDGEAELRLNSGAREVIVERTDDTNTVKVSVSGLLEVDVKVTPIKEKENKVHNYQLPSDDAFAHLETQFKFFDLSENVEGVLGKTFRPDYVSPVKRGVAMPMMGGEDKYQTPSLYSPVCRVCLFHRQSALAAAM</sequence>
</entry>
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        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DCAR_0415592</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF291">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="182"/>
            <end position="349"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG96258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="457" mass="51446" checksum="853D50B65D6CC4C2" modified="2024-05-29" version="1">MAPPSKGLTRKGKQEKEFTEEVEKNAGPNFQASLPSGSGSFNWWDQFSKRLRGSLSKSVDSANFELVFKMSRRTFKYICSLVEDAMIANSSNFTDLNGQPLALEDQVAIALRRLSSGESIQTVGHSFHIHKATVSNITWRFVEAMEENAIHHIQWPSTGAKMTEVKSKFEKLGGLPNCCGAIDTTHIKMRLPTSKTESNMAWYDKQKNHSMILQAIVDPDMRFLDIFAGCPGCLSDFIALKESGFFKLCNEGKRLNGETVKLPEGTELREYIVGDLGFPLLPWLLTPYRGKNLPENQASFNRRHTATWEVAQKALSLLKDTWKIIDGEMGQRDKDRLPEIVLVCCLLHNISIDLNDEVQKEIPITYVNDPEYRQQICDSVDNAAAGMRSELSLYLSGRMGPSEGLTRKEEQENDITEEVEKNAGPNFQASLPSGSDSFNWWDHFSKRLTGMSLEFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WXY8</accession>
    <name>A0AAF0WXY8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416290</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="13">
        <location>
            <begin position="16"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="277"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="628"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="710"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG96951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1052" mass="120948" checksum="8782905CA609A09A" modified="2024-05-29" version="1">MGDSRVYSGADRAGIVSKIRLENFMCHSNLEIQLGDFINFVTGQNGSGKSAILTALCVAFGCRARGTQRAASLKDFIKTGCSYALVQVELKNEGEDAFKPETYGDSIVIERRISESTSSTILKDHQGKRVASRKEDLVDIVEHFNIDVENPCVIMSQDKSREFLHSGNAKDKFKFFFKATLLHHVADLLKSIEKQLIGAQELVGNLESSISPILKELSELQGKIKSMEQVEEISQRAQLLKKKLAWAHVYDIDDQIQQQDARIEKLKGRIPYCQDLIERQTVKIEGLEDRLAKKKAQIAILMERTSEVRKMKEELQQKRSEATKKRSELEEDCSRRVNEIKRMMKKAQMLEQQIRDINDQDDKNTQAEEHEMEETLSGLRQEVEEAVMQFERCKKDEESLSNSIADADNDIRKLQSAIDDLMKKDQQIRYTIRDLRSHQTNKVTAFGGDRVTNLLRAIERHHHRFGRPPIGPIGVHLKLVGGDKWAYAVENAVGRLLNAFIVNDHKDNLVLRACAREANYNHLQIIIYDFSRPRLNIPDHLLPQINHPTTISVLRSENATVMNVLIDVGHAERQVLVSDYSVGKSVAFDRNIQNLKEVYTLEGYKMFSRRSAQTTLPPNRNVRTGRLCSSFDDQIKNLENDASHIQEEVRQAREKRKRLEDGHHNLRDEFQLAKRRRTDVEGILRSKKFALQDTEKSYALESSSRPTSNVDELYQELSLAKKEIQERESKLEEIQVSAAEAKIKASELKASFENLCESAKAEIHAFEKEEQDMMQIEEDLHGAKAERNRFELAKNERVIPNIKEAELRKEDLENNRKVNYKKASIICLESEIQALGGNDGSTPEQLSAQLTRLNQRLQRESQNYPESIDDLRMMHDKKERKIFRKRQTYKMFRQKLHACQKSLELRYSKFQRNATLLKRQLTWQFNGHLRKKGISGQIKISYEDETLSIEVKMPQDASNNTVRDTRGLSGGERSFSTLCFALALHEMTEAPFRAMDEFDVFMDAVSRKISLDTLVDFALAQGSQWIFITPHDISMVKQDERIKKQQMAAPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY39</accession>
    <name>A0AAF0WY39_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416350</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF216">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="NAC" evidence="13">
        <location>
            <begin position="9"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="517" mass="57609" checksum="8BF77440A3436440" modified="2024-05-29" version="1">MAVRPEGNLPVGYRFRPTDEELINHYLRLKINGFHSEVSVIREVDVCKCEPWDLPDLSLIHSIDNEWFFFCPKDRKYQNGQRANRATVAGYWKATGKDRFIKSGKGLNVIGRKKTLVFYTGRAPRGERTHWVIHEYSATDEALSGTRPGQSPYVLCRLFKKHDGKQDEMSESLNCEEVEQVVLSPTVFKPSHEDALSEQETQLLSRQPDGYETNHETPSTIDQYSFQGNTCISEEADGQLLDMTSIQPNPDPENAFGDFSEVPDHDSKIFSPCHMQMMEDYYHNSTVLGNDNNEDIDIASFLDSILATSDDCSYEDSASHPIPAIGNESPKYIGSIDGSFVKGSGSGSESDMELLHGQQNIPSTMMLRSTIGLAAPEVRNEEAQRNMQLMQNRADVSSTDVSTSNGIKIRSRQPKIVQQSAQNVMAHGTAPRRIRLLMNPQVGSNYGSLTDENAEAELPLIEDEQDSQESSFNVFSKASFMFSKAPPFLSSISSALHIFLVLGVVLVAVAFCGCFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY55</accession>
    <name>A0AAF0WY55_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0415999</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="92"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG96663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="27280" checksum="C0A23985B1DECDA4" modified="2024-05-29" version="1">MSGLSMSLDDIITMNKHKQQQQQRLSSPPPHSGAAPSRRFFNRTVHRSFPYPRSHVEAPEMEWKHDMFAQYAPAHISYPPMLNRLYYVETATKILVSNLNYAVSENDIKDLFSVVGDVKKISVHYDKSGRSEGSAEIIYSNWKDAEAAVKRYNNIQLDGKPMEVKIVGMVTATSAVMSPYGCYGLRRDVGKGSELVGLSQGGNMHTSGRGLRRWNGGKAYKNLSAEDLDADLDNYHAEVKQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZQ2</accession>
    <name>A0AAF0WZQ2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0417978</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the early nodulin-like (ENODL) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG98634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZQ2"/>
    <dbReference type="KEGG" id="dcr:108218797"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11019">
        <property type="entry name" value="OsENODL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000010">
        <property type="entry name" value="Early nodulin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041846">
        <property type="entry name" value="ENL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF185">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042294591" description="Phytocyanin domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="13">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG98634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="25126" checksum="E6AD399B99E71C6F" modified="2024-05-29" version="1">MDCLRVILFSILLVSTICSSAEALNFNVGGKYGWSLYASGNYNQWANMMRFQVNDTLTFKYKKETDSVVVVTKDDYEKCNAEKPIQKMDGGDSVVKLDRSGPFYFITGNKSNCQKGQKLLIVVLAVRDKAPPTPAVPAPAPAPENIAPVPSPATKAPSPSPSSESPADSPPPAISPAPVTPSPAADSPGIVPTPSPLSPGNSPWNMSPAPAPSSSFAPAMSPAMLVTAVLGVIVTSYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4H9</accession>
    <name>A0AAF0X4H9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CHLORORESPIRATORY REDUCTION 6, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X4H9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010275">
        <property type="term" value="P:NAD(P)H dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014946">
        <property type="entry name" value="CRR6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038024">
        <property type="entry name" value="CRR6_slr1097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35724">
        <property type="entry name" value="PROTEIN CHLORORESPIRATORY REDUCTION 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35724:SF1">
        <property type="entry name" value="PROTEIN CHLORORESPIRATORY REDUCTION 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08847">
        <property type="entry name" value="Crr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH00985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="20236" checksum="5FB708CE0738898A" modified="2024-05-29" version="1">MPPKEGRYEVTIDNHDIRSLDLTPFQTTVGFTSPSSVEPQEFLERTIGFTINYTKEDPYDPRELSEYPDIRLWFLRLDATYPWLPVLLDWRAGELARYAAMLVPHQMSMRMGVVFNPEALELFVMEKVFVVYSWLKQHDIPKPRLKAKDMARMLGFGIGDELFDLIDKHHVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4M9</accession>
    <name>A0AAF0X4M9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0520685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X4M9"/>
    <dbReference type="KEGG" id="dcr:108220278"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="234"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="269"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="374"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH01303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="65734" checksum="1F5FC00DCCD0203B" modified="2024-05-29" version="1">MMMIRAFTTSAAVARTTTTTTRAQLQQLLLQKSKVGFDKLDDALVVFDKMLLMKPLPTVIDFTQLLAALVKMQQYAFAVSLFRDMRVLCIPANIVTFNTVINCCCHLNRLDFAFSFLGGIIKCGLVADVFTYNTLIRGLISQDKFVEAEHLFKKLIRFDDMKHDVVSYNTIINGLCKTGHTNMAVKLFKYMKKKGCQPNTVTYSTIIDSLCKHRLVDEALGLLCEMTRKGVLADVVTYSSLIQGLCDFSRWDDISKLLREMEIKKISPNVHTYNILIDAYGKEGKMKDAQDWVEIMVQRGLYPNEVTYCTLMDGYCLRGEVDKALEVLETMKSKGISHNTYSYNILIKGYCKKLKVDNAIDLFQQMPCEGLTPTIQTYNTILQGFFDSGRHVEARKFYKEKMLNLGIHLDKVTCLIMLHGLCKNRCVVEALSLFHMMESSGLVPNIYVYSILIDGLNKNGHLEKAKSLFDSLPSKGLKPSVKTYTMMIRAYCEEGLLDEANKLIGQMEAENCLPNECTYNILIRGYFRYKKYHEGGALIDKMRGRGFSADASTTSMLLDLLELKEHDPALHALQKKFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5Z0</accession>
    <name>A0AAF0X5Z0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0521800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5Z0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001722">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF932">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH02411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="173" mass="19225" checksum="0FF3EA86ABE0B3CC" modified="2024-05-29" version="1">MNPKISDFGMARSFGGNQMQANTTRVVGTYGYMPPEYAIDGIFSTKSDVYSFGVLVIEIISGKKNRSFVHPDHSLNLLGHAWTSYKEDKLSGLVDEAISESCNHAEVFRVIQIGLLCVQQHPADRPSMSHVVLMLSSNIALPHPKQPGFFMERTFHDPDSSSNQLTITVLEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7Z7</accession>
    <name>A0AAF0X7Z7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0622859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X7Z7"/>
    <dbReference type="KEGG" id="dcr:108226931"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31288:SF10">
        <property type="entry name" value="PROTEIN ESMERALDA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH03461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="557" mass="63119" checksum="071A423338301B3E" modified="2024-05-29" version="1">MHAYNRLPGSGQTTPSPSPPSSPRSPRHRQPRSKPKPHSTLAQRLSWFLISFLLRRQRILLLAPFLYISVMLFYTGKLSLDHVIPLAIKSRLSPGSVYRSPQLYAKLRPHMDADNSSMDAISTIWNHPKEGEWRPCTNTSSGGLPESNGYIYVDANGGLNQQRTSVCNAVAVAGYLNATLLIPNFRYHSIWRDPSKFREIYDQSFFISTLVNDVRVVDKVPGYLMERFDQNMTNVANFKVNAWAPVDFYKDIILPRLLEEKVIRISPFANRLSFDAPPAVQKLRCLANYEALRFVSPILTLGESLVAKMKELSANNNGKYISVHLRFEEDMVAFSCCVYDGGKKEYEDMNTARERGWKGKFTRPGRVITPGAYRINGKCPLTPLEVGLMLRGMGFSNSTAIYLASGKIYDSERHMAPLLEMFPLLQTKEMLASEELAPYKNFSSRMAAIDYTVCLHSEVFVTTQGGNFPQFLLGHRRYLYGGHAKTIRPDKRKLALLFDNPKMGWKSFKRQMLTMRAHSNAKGFELKRPSDSIYSFPCPSCMCRVNKKINSNTSSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8K7</accession>
    <name>A0AAF0X8K7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">holo-[acyl-carrier-protein] synthase</fullName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0521708</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8K7"/>
    <dbReference type="KEGG" id="dcr:108219815"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000003">
        <property type="entry name" value="L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000013">
        <property type="entry name" value="L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055066">
        <property type="entry name" value="AASDHPPT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22624">
        <property type="entry name" value="AASDHPPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="3">
        <location>
            <begin position="118"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="307" mass="35617" checksum="C849A6F27C7CF133" modified="2024-05-29" version="1">MEMEKGVKRWLVDISNWNPSPLAFSHAISLLPPYTHSSITRFVKLEDQKRTLVSRLLQYTLVHEVLQIPIAEILINRTLEGKPFLEYKKSKLAFPNFNFNVSHHGDYVAIASEPICLVGVDIVSHSTPEKETVLEFIQNFSTYFSSLEWDKIYKAGSDDDILLEFYSLHFHRYWCVKESFVKAIGNGVGYKLDGVEFHHREWTNIRVNINGAELKDWKFWLSSIGKNHMLSVARGHPRIAAESYRKTLDKTQFDENEYKFGLNLPNSSFVLRTVEQLISIFLQSPKITRNMSRKSDNSEDRSHGQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X928</accession>
    <name>A0AAF0X928_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0522121</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X928"/>
    <dbReference type="KEGG" id="dcr:108224088"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF114">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="9"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02732.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
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    <sequence length="446" mass="50686" checksum="D74599CC1E926ED8" modified="2024-05-29" version="1">MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKILDRSTILKHKMVDQIKREISIMKRVRHLHVVRLHEVLASRTKIYIILEFITGGELFDKIVHHGRLSEAESRRYFQQLIDGVGYCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSALPEEGVSLLRTTCGTPNYVAPEVLSHKGYDGALADAWSCGVILYVLMAGYLPFDEMDLTTLYSKIERADYSCPSWFPVGAKSLIRQILDPNPQTRIHIEDIRNDEWFKKDYTPAGFLEYEDINLDDVNAFLDDSEGERPDEQCRDEDAGPLNLNAFDLIILSQGLNLSALFDRGQDSVKHQTRFVSQKPAKLVLSSMEVVAQSMGFKTHIRNYKMRVEGLSENKASHFSVILEIFEVTPIFVMVDIQKAAGDAGEYLKFYKSFCNNLEDIIWKPPSEYTKSRITKAKSKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XA23</accession>
    <name>A0AAF0XA23_DAUCS</name>
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        <recommendedName>
            <fullName evidence="13">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0522638</name>
    </gene>
    <organism evidence="11 12">
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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                <person name="Macko-Podgorni A."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        </location>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28166" checksum="E79C40B4471C0564" modified="2024-05-29" version="1">MDPEINLQDLHPKKRWRSELYAQFVLAWQNNNQLPAEGTPEKNVNSLDDNHQLAVYTPADASASDGTVTQPPQNQAGRIAPSVEAYTVQCSLCYKWRLIPSKEKFEEIREHLIEQPFVCRALYQWRPQISCTVKTDLQPDGSKLWAIETPNIPRPPPRWKRILKIRASQGTRFADVYYYTPSNKRLRSTTEVQRYLREHPNEADGVDLGQFSFKIPLLVKEKSLEEHTTRVLQDGRKRRRMP</sequence>
</entry>
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        </recommendedName>
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        <lineage>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
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                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="10">
        <location>
            <begin position="144"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="258" mass="29421" checksum="F1C49A3E03B8C671" modified="2024-05-29" version="1">MGACNTFSNMKSLNLDSIRESLIRQEDTIIFSLIERSQFPMNSPVYTPALASTLPSSKSLLHFIVNQSETIQSQAGRYESPEEHPFFPDNLPSSLLPQYNHPQILHPTAAAININKTIWDVYVKQLLPLFTAEGDDGNYVPTATSDLQCLQAISRRIHYGKFVAEVKYLDAPEDYAPAIRAQDRDALMKLLTFESVEEMLKRRVEKKAKVFAQEVSLNVVDQKEKYKIDPSLLPLLYGEWIMPLTKLVEVEYLLKRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XET3</accession>
    <name>A0AAF0XET3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0625152</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XET3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000054">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000175">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000081">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041915259" description="Glutamate receptor" evidence="16">
        <location>
            <begin position="22"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="17">
        <location>
            <begin position="469"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="760"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH05732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="912" mass="101901" checksum="C7B588592D691BE6" modified="2024-05-29" version="1">MRTSVVILSIWVLAQVIDVNGETNNSYSRPNVVNFGALFTLNSVIGRSVKPAIAAAIYDVNSDSSILPGTKLNMILHDSNCSGFLGTIGALQLMENDVVAVIGPQSSGIAHVISHVVNELHVPLLSFGATDPTLSALQFPYFLRTIHSDYSQMHAIADLVQHYRWNEVIAIFVDDDYGRNGISALGDALAEKRAKISYKAAITPGASATDINNLLAGVNLMESRVYVVHVNPDSGLTVFAEAEKHGMLASGYVWIATDWLPSVLDLSDSVDPKTMDLLQGVVALRHYTPDSYLKKRFSTRWKQIKGKETVNFNSYALYAYDSVWLLARALDVFFNEGGNISFSYDSKLQDGKGSKLHFSELRSFDQGNILTQILAQINFTGLTGHFGFDHNKNLINPAYEIINIGGTGSRRIGFWSNYSGLSIEPPDILYKKPLNISGMDHHLYSVIWPAETTTTPRGWVFPNNGKPLRIAVPYRVTYKEFVTKDNGPTGVHGYCIDVFEAAVALLPYPVPRSYLPYGDGLRNPSFSNLVYDVSQNKYDAAVGDITIVTNRTRIVDFTQPYMESGLVVVTPVKKVKSTAWAFLKPFTWEMWGVTGAFFLFVGVVVWILEHRINHEFRGPLSQQIVTILWFSFSTMFFSHRENTVSTLGRLVLILWLWVVLIINSSYTASLTSILTVQQLTSQIEGIDSLISSNVPIGVQDGSFAFNYLIDELNIAKSRIQIMKSQDDYIYALHRGPKGGGVAAIVDELPYIKLFLSNTNCEFRTVGEEFTRSGWGFAFQRDSPLAEDLSTAILQLSENGELQRIHDKWLSKNTCSESTNQVDITRLSLTSFWGLFLICGTSCFIALIVFFCRVYCQYRRFTPENDEEDPETGRSARRLPSRATSFKDFVDKKETDIKEMLKRKNSDNQPQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF91</accession>
    <name>A0AAF0XF91_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0625410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF91"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF45">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="7">
        <location>
            <begin position="379"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH05987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="652" mass="74896" checksum="FBF8556D0F388D4D" modified="2024-05-29" version="1">MAKDCKTPAPASNALRIMGTVPEVNEPPRPRVFDMSVKDAIQDAEVVTGTLNVNSINAKVLIDSGATRSFISQAFVDKLNCPVELLNEVMNVELANQDRISVNRVCPDCEIEILGNKFCADLIPFKLGEFDVILGMDWLSKHSAQIDCKNKKVILISPDNKVITFRGQKQVKKFLTMIQAKRMLRQGCEAYIAYVIDKSQEPLKLENIPVVNEFPDVFPDELPGLPPDREIEFAIDFAPGTEPISKAPYRMAPVEMKELAKQLQELLEKGVIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKLTIKNKYPLPRIDDLFDQLKGAEYFSKIDLRSGYHQLKIKSEDIPKTAFRTRYEVQFLGHIVSREGIKRFSLDKLVHMYLKEIVVRHGVPVSIVSDRDPRFNSRFWKNFQECLGTKLNMSTAYHPQTDGQSERTIQTIEDMLRVCAIDFKGNWDEHLPLVEFAYNNTLYGRKCRSPVYWDEVGERKVLGPELVQQTKEVVEIIQKRLVAAQKAVLLKVSPWKGLSRFGKKGKLSPRYVGPFEILRRVGKVAYELALPPQMEHIHSVFHVSMLKKYNPDSKHVIEYEPVELEADLSYVEMPVEILDRKEKVLRNKVVKLVRVLWRNPKVEESTWELESDMREKYPHLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHF7</accession>
    <name>A0AAF0XHF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0626641</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHF7"/>
    <dbReference type="KEGG" id="dcr:108227409"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000014">
        <property type="entry name" value="Kinesin-like protein KIN-7G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7K, CHLOROPLASTIC ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="69"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="526"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="390"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="749"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="830"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="948"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH07212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114050" checksum="7528BD4C61337FBB" modified="2024-05-29" version="1">MAYRQGVKSKKLNSRAANSPSSSTTSSSKQFLETSVEGLSSPASSTSRSKPHFYYSETTPIDVQRSKENVTVTVRFRPLSQREIKLGEEIAWYADGETIVRSENNSSIAYAYDKVFGPTTTTRQVYDVAAQHVINGAMEGINGTIFAYGVTSSGKTHTMHGDQRSPGIIPLAVKDTFSIIQETPSREYLLRVSYLEIYNEVVNDLLNPAGQNLKIREDAQGTFVEGVKEEVVLSPAHALSLIAAGEEHRHVGSTNFNLLSSRSHTIFTLTIESSPCGEHREGESVNLSQLNLIDLAGSESSKAETTGVRRKEGSYINKSLLTLGTVISKLGEGKASHVPYRDSKLTRLLQSSLSGHGRVSLICTVTPSSSNSEETHNTLKFAHRAKHIEIQAAQNKIIDEKSLIKKYQHEIRTLKEELEQLKRGIVTIPQLKRTEGDDIVILKQKLEDDQVKLQSRLEQEEDAKAALLSRIQRLTKLILVSTKTSQASRFSHRPGPRRRHSFGEEELAYLPLRRRDLNLDDQNIDTYTSVDGNFETADETPKEEKKTRKNGLLNWLKPRKRDNGSRTFMSTSEKSSGTKSMSTPSTPKAESGNLLMESRLSQKAENTPSSDLLSDARHGRETPEESLQHETPLTSIHTLDQIDLLREQQKILSGEVAFHSSVLKRLSDEALRYPKKEYIQEEMAKLQGEIRKKNEQIALLEQQIAGSVISSMDNLDQLELSESVADHVADLVTRLNEKSFELEVKVADNRVIQEQLSQKIDECEKLQQTVLSLQGQLSEARELKNLSQMIDNSIRFPETEKVNMENSLMNKTAVSKDVYEGSFLKAQMEIGELTVKVTELTESREKLEIQNQQLAEESSYAKGLASAAAVELKALSEEVAKLMNHNERLTAELAAQKNSQTQRKTFAANRNGRRDVNIKRHDQGIVNPEVKRELALLREREHSYEAALAEKDQREAELQRRVEESKQKEAYLENELANMWVLVAKLKKSQGPVNASELTRESSIVEDDYKIWNGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XI19</accession>
    <name>A0AAF0XI19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0626224</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XI19"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="7">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="8">
        <location>
            <begin position="290"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="411"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="431"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH06796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="57757" checksum="AF4086D545002DE5" modified="2024-05-29" version="1">METYTLLKDLTLGRGTGFIKVRISREWEGRKPGATHATTKTYIIIDEEGTQVQAGPLQFGLIAEFSKRLLLGSVYLISNYDVAAAPDTYRPVPGEYTVNFHRKTSVKKIGDVPAIPMFQFNLKTFEETKARLGDVVTLIDVVGKLKDYTHIQTAKSGKKSLDIVLADKRDEIKVTLWENQAFEFLKLENEYTRANVIVIITGTSTRLVKGEFVLWSSSSTQYFFNIDHSAVTTLRESTKLENSIIPTLVPSMKSQEQQNMANIETVTIAQLFEAQLPDGKNFIEFYSEATVIGLFPNEGWYYIGCNKCGKKMNDFGQCIKCSHKTKPIPIYKVTLAVKDSTADTSFVIFDRHVMKLIKVSAQHLLNSDQNATPEMMPPILNNMVGRTCIFRLKLNQYNTVQHKEGFTVMEVDDVKSNKPTSNSKIDSGEDSSEHDLDHESTEPSEHHLQKKRKKPNDDKYSDQVQPPPENTSKGSLEVEQGHDAQRHAKNGGQVPPPNSGTKGSPGNKSLKRTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XI46</accession>
    <name>A0AAF0XI46_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0626254</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Also involved in chromosome segregation in meiosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: CAPH, CAPD2 and CAPG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XI46"/>
    <dbReference type="KEGG" id="dcr:108227484"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000005">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000278">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="16">
        <location>
            <begin position="518"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="393"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="736"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH06826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1175" mass="132463" checksum="EEE40ADF5A6A9AF4" modified="2024-05-29" version="1">MHIKEVTLEGFKSYATRTVVPGFDPYFNAITGLNGSGKSNILDSICFVLGITNLQQVRASNLQELVYKQGQAGITKATVSVVFDNSDRSRSPFGFEDFPEITVTRQIVVGGRNKYLINGHLAQPSRVQNLFHSVQLNVNNPHFLIMQGRITKVLNMKPPEILSMLEEAAGTRMYETKKEAALKTLEKKQNKVDDIDKLLDVEILPALDKLRKERTQYMQWANGNAELDRLKRFCIAYEYIQAEKIRDNAIQGVEVIKTKLSEIDANTEQMKREIQEMETKVSDLTAEKEARMGGEIKCLSDKVDALSRDLVKETSVMKNQEDNLMIEKDNVLKIDRNIEELKQSAKEKALAVQSAEDGAADLKGRVGELSKSLVDHEKEYQGVVAGKSSGDEEKCLEDQLADAKVAVGRAETELKQLQTKISLGEKELKAKSKQLLSKREEAVAVEKELIVRTKDVEDVKRALESVSFEEGKMEALQKDRTAELDIVQKLKDEIRMLTSRLGNVDFQYRDPARNFDRSKVKGVVAKLIKVKDRSAMTALEVAAGGKLFNVVVDTENTGKQLLQNGELRRRVTIIPLNKINSHTVPPRVQNAAVRLVGKENAEVALLLVEYDKELKSAMEYVFGSTFICKTTDAAKEVAFNKEIGTTSVTLEGDILQPSGLLTGGSRRGGGDLLGQLHALAEAELNLGHHQKCLSEIEAKISELLPLEKKFNDLKKQLELKLYDLSLFQSRAEQNEHHKLSELVKKIEQDLEEAKSSINEKQQLHKNCVAQVSLLEQTIYEHANNREGRLKILEKKIKEIKGKMQSASKDLKNHENERERLVMEMEDVHKEQTSLENQLASLGKQIANLTSEVEVVKKKVASLGNDYDQAQSELNIVRSKLRECDTQISFILKEQQKLQHKITENNLERKKMENEVIRMEMEQKDCSQKVDKLIEKHAWILSEKHLFGRGGSDYDFKSCDPYKASEEYAKLQVEQSGLEKRVNKKVMAMFEKAEDEYNALISKKNIIEMDKKKIRKAIEDLDEKKRDTLKVTWEKVTSDFGSIFSTLLPGTTAKLEPPEGASFLDGLEVRVAFGGVWKQSLSELSGGQRSLLALSLILALLLFKPAPLYILDEVDAALDLSHTQNIGKMIKTHFPHSQFIVVSLKEGMFNNANVLFRTKFVDGVSTVQRTVASKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIB3</accession>
    <name>A0AAF0XIB3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="6">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0727040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="6"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIB3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH07607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="241" mass="27337" checksum="7244902B8B349361" modified="2024-05-29" version="1">MPILMEYMEAGSLQNLVKSNGTLSEKMIAEVARRILMGLDYLHNTKHIVHCDIKPANLLVNHNMEVKIADFGVSKLIDLASDESHVFSGTTAYMAPERFDSCAYGDDLDVFAGDIWSLGLTLMEIYIGHQPYFAPHRKPNKTEFDLMFDVCYNDSPALPEEASSDFQDFIRCCLEKNPSKRWKAVQLLSHPFLINKLDDQDAPIAPDKSNAEPVDSKGRKREANIEIRESSKRMKRQDVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJW1</accession>
    <name>A0AAF0XJW1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0727852</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJW1"/>
    <dbReference type="KEGG" id="dcr:108196251"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031349">
        <property type="term" value="P:positive regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000248">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL27"/>
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    </dbReference>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0611">Plant defense</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="86"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="371"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="499" mass="55099" checksum="412E93FAA5192822" modified="2024-05-29" version="1">MGCFPCFGSSEDDGKNGEQEVVKKDSFKEGSAPQSYHMTRVGSDNTKSRGGFDPKKELPLPKDVPTPHIAAQTFTFRELAAATKNFMPECLLGEGGFGHVYKGRLESTGQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLPPNKEPLDWNTRMKIAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGEGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARTHGEHNLVAWARPLFKDRRKFPKMADPLLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQAYDPNASVSQSSRVGSSTPRSRDRRSTHDAVDSPGHNGSPTTHKNSPDFRKRDLMTNSRNGETSGGSGRRWAVDDLEYTESQRDSPVSAGRTREPRNRDLDRERAVAEAKVWGENLREKKQGGAMDSFDATNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMG1</accession>
    <name>A0AAF0XMG1_DAUCS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0728636</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMT1</accession>
    <name>A0AAF0XMT1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">mRNA guanylyltransferase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="20" type="ORF">DCAR_0830472</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108199385"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14502">
        <property type="entry name" value="RNA_5'-triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000188">
        <property type="entry name" value="mRNA capping enzyme family protein"/>
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        <property type="entry name" value="mRNA capping enzyme, putative"/>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
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        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
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        <property type="entry name" value="mRNA_cap_enz_bifunc"/>
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        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
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        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
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        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="119"/>
            <end position="268"/>
        </location>
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            <begin position="187"/>
            <end position="256"/>
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        <location>
            <begin position="446"/>
            <end position="594"/>
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            <begin position="25"/>
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            <begin position="283"/>
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            <begin position="32"/>
            <end position="64"/>
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            <begin position="65"/>
            <end position="78"/>
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            <position position="211"/>
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        <location>
            <position position="382"/>
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        <ligand>
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        <ligand>
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            <begin position="428"/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQF3</accession>
    <name>A0AAF0XQF3_DAUCS</name>
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            <fullName evidence="2">Protein FAR1-RELATED SEQUENCE</fullName>
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        <name evidence="4" type="ORF">DCAR_0830729</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <comment type="function">
        <text evidence="2">Putative transcription activator involved in regulating light control of development.</text>
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        <text evidence="2">Belongs to the FHY3/FAR1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="196"/>
            <end position="232"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="391" mass="45439" checksum="95D2668D6748926A" modified="2024-05-29" version="1">MKAALQISFPLLGTVFCAESGFMDKLGRVIWADHITPDEFEQGWIDAVDEFDLNENVWLKEMFDMRSLWIPAYFNDEPMVGLMQTTSRSESFNFYFGNYVQRGDTLSEFYICYQSAIEKQRNNAKKLTHYDDFTPKTITDREIEKDAIRLDTRDLFYKVQEEIRAGCMDIILLGMTCLDNVKKIKIQEPGNRTRIFEVELNMETHFIECSCKLFTRVGYLCSHAFFCLGICGIRIIPRQYVSNRWLKNTVERFSTLELGEISDRDATILSRHVQTQDCWFEFQGCLSDASGNADVLEYIKNGISSMRKHITTTMKKPRTRLNSEQIEELIDSHVVNEITIRNPNKSNNKGSRKRIISGAEKSIGCNNRKMRKCATFQEYAYHDSRTCPEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTA6</accession>
    <name>A0AAF0XTA6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0832257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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        <subcellularLocation>
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    <comment type="similarity">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
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    </dbReference>
    <dbReference type="Pfam" id="PF05859">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0132">Cell division</keyword>
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    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    <sequence length="241" mass="27390" checksum="5427DB4FD222CDCC" modified="2024-05-29" version="1">MEGSESEAIFDSLNLNPQLFLNEIFNRLDDLVDGAFHCFHQEASTGLKIEGTDRADELKKGVVYLQNRIHSALDRPLTMWEKYCLRHCFAVPLGFTLPANNESTGEVAMDVDVTDDTELDLQLNSLRKRLVLVGKESAELNRELRMLERQSSLSNQCSRSLDEAVQLCGQYFDLDMFEELKKTASQLRTKLEKLKTNRVEENDCIIMNRMHTSDGTLFGVNHGNGLANTTLEELQQLMNDV</sequence>
</entry>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
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</entry>
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    <accession>A0AAF1AJC3</accession>
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG82260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108203538"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="109" mass="12683" checksum="21426707BA192A90" modified="2024-05-29" version="1">MQRQRSLSASEAPTAKKKAKKMRKEEAAADLGGERLDYMAVAAMDIVLTEWEERCSAWSWMLGDDHMMASWCPLWDVQLMGNAYQDYLFDEVVWDDDIWNIKHINNIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQH5</accession>
    <name>A0AAF1AQH5_DAUCS</name>
    <protein>
        <recommendedName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0207886</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108209637"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:fucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Bifunctional fucokinase/fucose pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012887">
        <property type="entry name" value="GDP_fucose_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052203">
        <property type="entry name" value="GHMP_Kinase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001174">
        <property type="entry name" value="HddA/FKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463:SF0">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07959">
        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00960">
        <property type="entry name" value="LMBPPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDP-fucose pyrophosphorylase" evidence="7">
        <location>
            <begin position="137"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="829"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="8">
        <location>
            <begin position="990"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG88651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1078" mass="118360" checksum="C3596734F6014043" modified="2024-05-29" version="1">MEDKKKWGKRNEKAELSEILRKSWFHLRLSVRHPSRVPTWDAIILTAASPQQAQLYEWQLARAKRLARIAPSTLTLVVPDPHACRIGSGAATLNAILALARHYQLNSDSNLNDKNSLGASSLHETSNNEVSSLMVNFLAKKHILLLHAGGDSKRVPWANPMGKAFLPLPYLAADDPDGSVPLLFDHILAISSCARQAFKNQGGILIMTGDVLPVFDAFTMVLPDDTSCIITVPITLDIASNHGVIVASKTGTFSESYSVCLVENLLQKPSVEELVKNQAILSDGRTLLDTGIIAVRGKAWADLVLIACSSQPMITELLKTRQEMSLYEDIVASWVPAKHAWLKQRPLGEDLVTGLGNHKMFSYCAYDLSFLHFGTSSEVLDHLSGIGAGLVGRRHLCSIPATTISDIAASAVIISSKIAAGVSVGEDSLIYDSSISGGIQIGSLSIVVGVNVPGVDGRFHNDTFRFMLPDRHCLWEVPLLEAGRVIVYCGLHDNPKISLAKDGTFCGKPWKKVLDDLNINEIDLWNSVGTQEKCLWNAKIFPILPYIEMLSVAMWLMGLSDRNNDTLFALWKVSTRVSLEELHCSIDFSRMCRGSTNHQADIATEIVKACLSYGIVGRNLSQLCEDILQKEASGVNICKEFLNMCPNLQAQNPAILPKSRAYQVQVDLLRACGNESVATEVEHKVWAAVSDETASAVRYGFKEDFLETSTKNLGIAYHENNLTGCLSEFFTKKVKVELPVRVDFVGGWSDTPPWSLERAGCVLNMAITLEGSLPIGTIIETTKTTGILISDEDLDQVFVEDFTSIKTPFEINDPFRLVKSALLVTGIIHDKILLSVGLKIKTWANVPRGSGLGTSSILAAAVVKGLLQITDGDESNDNVARLVLVLEQLMGTGGGWQDQIGGLYPGIKFSTSFPGIPLRLQVTPLSASPQLIKELQQRLLVVFTGQVRLAHHVLQKVVTRYLRRDNLLVSSIKRLAELAKIGRDSLMNCNIDEIGNIMLEAWRLHQELDPFCSNEFVDKLFAFADQYCSGYKLVGAGGGGFALLLGKDAASAKEMRLVLEENPEFDVRFYNWNIFLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AS81</accession>
    <name>A0AAF1AS81_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0312763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AS81"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056708">
        <property type="entry name" value="DUF7806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35489">
        <property type="entry name" value="TITAN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35489:SF2">
        <property type="entry name" value="TITAN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25091">
        <property type="entry name" value="DUF7806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7806" evidence="2">
        <location>
            <begin position="157"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="203" mass="23665" checksum="3D8E704B569F68FB" modified="2024-05-29" version="1">MNSLHHKLYEKYNQFKKKYITELFRHAAADELLQQYRLENERLVSENDDLRSELASIAEHQKLVMEENKKQKELLQKIERLQSVQREGLCCISRFEKNEKPNSLDRSQVGSDLPKCCIQKTALAGGDKNVLGSTICMFQELLECLFGMKVSIVTKADKHLLYHVLSLGTIESIAPEWMKEILMFNTKMCPVFFEKMSRVVKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATE9</accession>
    <name>A0AAF1ATE9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0313975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108211770"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="166" mass="18932" checksum="CEE53032FFD6D58C" modified="2024-05-29" version="1">MARSKDADKFDIVAWWTNYGSETPNLQKMALKIVSLTSSSSGCERNWSAFEGFHTKKRNRLDAERLNNLVYVQFNAKLANKKSKMKDKFDMLVASDASKAQGWIVEGADDDELSDDSTVTAEEGSTLPTATRKAFRELHEEDFESDDEDNDDDVIIEEEEDYVDQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU12</accession>
    <name>A0AAF1AU12_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0314246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AU12"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF221">
        <property type="entry name" value="(-)-GERMACRENE D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="26"/>
            <end position="205"/>
        </location>
    </feature>
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</entry>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUE8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="192" mass="22322" checksum="9D7C20274756D217" modified="2024-05-29" version="1">MSSSSTSSTERVAFRAQVLSRHLHPSPSSSSSPCLLEPRACLEYPPSTKAMDTKAMRKRKHRFRHESDGHESIAFDTKAMRKLMDDHNLEDRDWLYGLIIQSNLFNPRDRGGKVFVGPDYNQSKEQQRVMTMKRIEYLAECGVFKGYLTYSSPPSQLRRFALQEVADSFDHSLSIKIGVHFFLWYYMIVYVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AV61</accession>
    <name>A0AAF1AV61_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AV61"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG96119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="144" mass="17115" checksum="5A043751E7657184" modified="2024-05-29" version="1">MENHPWTILILCDRSSNKRFRKPRPLTFFFFFYHNDKNTQYKKFRLFGEVFFPCCILFGECTNNFCIINCLLCVYRFDYRIFMNSRTITGSIVSCQSGNKNFFFCRILVCLLTKFFKTPTANLDFHLIKAVDRSLPPICSLTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZI9</accession>
    <name>A0AAF1AZI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0519911</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AZI9"/>
    <dbReference type="KEGG" id="dcr:108221910"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042128692" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="37"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH00545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="217" mass="24411" checksum="758554886354E18C" modified="2024-05-29" version="1">MILLKVVLMISIVVLAYNNNISTCEAIWIEVGSTGVTKCVSEDIKQNVVVIADFSLVNLEDVSSQLLPVLSIKVTSPYGNNLHHQENVTHGQFAFTTTEAGTYFACFWVDEGHKATTSVTVSLDWRTGIAAKDWESVARKEKLEGIELELRKLEDAVDTIHQNLIYLKTREAEMREVSETTNGRVAWYSIMSIGVCITVSVLQVFHLKLYFRKKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B0H1</accession>
    <name>A0AAF1B0H1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Peroxidase</fullName>
            <ecNumber evidence="4 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0520889</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 18"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B0H1"/>
    <dbReference type="KEGG" id="dcr:108220972"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
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    <dbReference type="InterPro" id="IPR019794">
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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        <text evidence="3">Part of the Tic complex.</text>
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            <end position="24"/>
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        <location>
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    </feature>
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        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH02495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="150" mass="18321" checksum="B7B2ECECBEA471B8" modified="2024-05-29" version="1">MYEFNNGLILIFFAFNYIINIYTFNGEETDVEIETASETKGTKQEQKGFIEEDPSPSLFSEEKEDPDKIDETEEIRVNGKENTKDEFHFTEIRYNNSPIYKGIFWFEKPLLTFLFDYKRWNRLLRYIKNNRFERTTRKEMSQYFFLYMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2D3</accession>
    <name>A0AAF1B2D3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0520769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2D3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF11">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH01387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="240" mass="26887" checksum="0D3CF6241A6F505A" modified="2024-05-29" version="1">MEVLPQDCIAHVLSRTSPQDACQAALISQSMKDVANSDSIWEAFLPPDYREIISRSTPPVSFESKKELFMKLTSPLLIDGGTKTISLDRTTGKKCYMLCARELDITWSDNSLFAQVSELVMVCWLEISATINTRMLSANTYYGAFLVVKFANRAFGLDHLASEVLIQVGESKYEGSICLCHTKSPDRIRERSDGWMEIEIGYFHTEDADTGKEVKMRLREVKGVHLKGGLIVEGIEVRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B376</accession>
    <name>A0AAF1B376_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit 6B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B376"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000008">
        <property type="entry name" value="trafficking protein particle complex subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19539" checksum="40ADD7439527DB3C" modified="2024-05-29" version="1">MGKEVSESCLDILLIETVSSYTNGFYAGKPELVAQAVEAIGYQVGNQLCERYTMERSQFSDHLEAIKFICTDFWIKIFKKQIDTLRTDHKGTFVLRDNRFRWISRVSNPISEDSGATFPSTLPVNFAQSRKMHLYFPCGIIRGALSNLAIPCAVTADISSLPSCIFTIQIRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BBQ9</accession>
    <name>A0AAF1BBQ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA damage-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UV-damaged DNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0831582</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of light signal transduction machinery. Involved in fruit pigmentation and fruit nutritional quality. Acts as a negative regulator of fruit pigmentation. Probably acts by participating in the CDD complex, a complex probably required to regulate the activity of ubiquitin conjugating enzymes. Repression of photomorphogenesis is probably mediated by ubiquitination and subsequent degradation of photomorphogenesis-promoting factors such as HY5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Probable component of the CDD complex, which probably also contains DET1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DDB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BBQ9"/>
    <dbReference type="KEGG" id="dcr:108199412"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000070">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000003">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000182">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000267">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="11">
        <location>
            <begin position="16"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="12">
        <location>
            <begin position="389"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="10">
        <location>
            <begin position="747"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH12083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1089" mass="121179" checksum="F36FCCDE0EEFE57B" modified="2024-05-29" version="1">MSTYNYVVTAHKPTNVTHSCVGNFTSPQELNLIIAKCTRIEIHLLTAQGLQPMLDVPIYGRIATLELFRPHGETQDLLFIATERYKFCVLQWDPEAGEVITRAMGDVSDRIGRPTDNGQIGIIDPDCRLIGLHLYDGLFKVIPFDNKGQLKEAFNIRLEELQVLDIKFLYGCPKPTIVVLYQDNKDARHVKTYEVALKDKDFIEGPWSQNNLDNGADLLIPVPPPLCGVLIIGEETIVYCSASAFKAIPIRPAITRSYGRVDADGSRYLLGDHNGLLHLLVITHEKEKVTGLKIELLGETSIASTISYLDNAFVYVGSSYGDSQLIKLNLQPDAKGSYVEALERYVNLGPIVDFCVVDLERQGQGQVVTCSGAFKDGSLRIVRNGIGINEQASVELQGIKGMWSLRSATDDPFDTFLVVSFISETRILAMNIEDELEETEIEGFSSQVQTLFCHDAVYDQLVQVTSSSVRLVSSTSRELRNEWKAPPGYSVNVATANSTQVLLAAGGGHLVYLEIGDGILNEVKHAQLEYDVSCLDINPIGGNSSYSSLAAVGMWTDISVRIFSLPDLNLVTKEHLGGEIIPRSVLLCTFEGISYLLCALGDGHLLNFLLNLTTGQLTDRKKVSLGTQPITLRTFSSKSTTHVFAASDRPTVIYSSNKKLLYSNVNLKEVSHMCPFNSAAFPDSLAIAKEGELTIGTIDDIQKLHIRSIPLGEHARRICHQEQSRTFALCSVKYNQSSAEDTEMHIIRLLDDQTFEFISSYSLDQFEYGCSILSCSFSDDNNVYYCVGTAYVMPEENEPSKGRILVFLVEDGKLQLVAEKETKGAVYSLNAFNGKLLAAINQKIQLYKWMLRDDGTRELQSECGHHGHILALYVQTRGDFIVVGDLMKSISLLIYKHEEGAIEERARDYNANWMTAVEILDDDIYLGAENNFNLFTVRKNSEGATDEERSRLEVVGEYHLGEFVNRFRHGSLVMRMPDSDVGQIPTCIFGTVNGVIGVVASLPQDQYSFLDKLQSNLRKVIKGVGGLSHEQWRSYSSEKKTADAKNFLDGDLIESFLDLSRSKMEEVSKAMTVPVEELMKRVEELTRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCC2</accession>
    <name>A0AAF1BCC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0933968</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCC2"/>
    <dbReference type="KEGG" id="dcr:108202471"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH14449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="493" mass="56796" checksum="4E15D6153BBCE437" modified="2024-05-29" version="1">MAQRFMETYGYDMILGSIAAFYVVMIPYTKVEESFNVQAMHDILYHQHHIENYDHLDFPGVVPRTFIGALLVSSLASPLIYVMNLLQLPKIYGLLAVRLALGCIILLTLRFFRVQIRNKFGRQVEAFFVIFVSIQFHMLFYCTRPLPNILAMGLVNMAYGYWFKGSFYAALNCLIFATVVFRCDILLLLGPLGLELLLTNSISFWKALRYCIGAALFSIGLTVLVDSFLWRKPLWPEFEVFWFNSVLNRSSEWGTQSFHWYFSSALPRSLLAAYPLFALGVLLERRLLFYVLPVFSFILLYSKLPHKELRFIISSIPIFNLSAAVAASRIYNNRKKSLWNLLYIVMLGLLIISLGGTMTTFMASYENYPGGSALKELHNFGYVTNSTDEVRVHIDTFSAINGVSRFCENGFPWRYSKEEEIPLDEYCHRNFTYLLNEHHNIKGFKCLFSVNGFSRLRLQTGFPQVSLITEPKVYVHGSITNKYITHQNWPGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD65</accession>
    <name>A0AAF1BD65_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BD65"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000079">
        <property type="entry name" value="Trehalose-6-phosphate synthase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF14">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="722" mass="81659" checksum="80B1CA6292001910" modified="2024-05-29" version="1">MDTSSCMNFANLAFGDLNRTNPCIKYVDSDGTSSTTVRIIIFSNMLPLHAQKDGRTEGFSFIYDESPLWQLKYGFSPDTMVFYVGSLNADIDVNVQEEVVERLMDDFNCVHTFLANELFSEFYHDFCKHHLWPKFHYMLPMCGKHGDQFDQGLWQAYISANKILANKVLEVANPEINYILIHDYHLMAVLVFLRNKRYRAKLGFFLQSPFLASAEIYRTLPVNADILRILLNCDLIGFHTYDFACHLLSCCSRMLGLDYESKRGQIGLDYSGRTVYIKILPLGIHLRKVENVLNIPSTSMKVKEIEGKFKGKHVIVGVDDMNLFKAISLKLLAFEQLLRKYENLRDIVVLVQIINMELLTGLIRLTSVLIIYEYVGCSPSPSGAIRINSWDISSLAEAMRSAVSMDDSLRQLRHEQNYSYVQSHDVAYCARSFLHSITRACLDHYNYQCWGLGFSFTFKVAALSLGFQKLFSKSIVPAYNRTNRRAIFLDFDATLVPHSSTKKNLSSELNTVFIVSGRGISSLTEWLAPCEGLGLATEHGYFIRWKKTSEWQSSLVVDLEWKVAVERIMNLYAEATDGSTIEVKESVLAKDLPDHLKHVIANEPAVVAKGKNIVEVKPQDASKGLVTEKVIATMVGNGEIPDFILCIGDDRLDEDMYDGTLKIVSSVVVPGVPEIFFCTVEQKPSKAKYFVDDTFEVQKLLQWLANVSSTQPSSAEYPPSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-07-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1TIA0</accession>
    <name>I1TIA0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small ribosomal subunit protein uS13m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Ribosomal protein S13, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rps13</name>
        <name evidence="14" type="ORF">DCAR_0M036220</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="13"/>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Plant Biol." volume="12" first="61" last="61">
            <title>De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Senalik D."/>
                <person name="Szklarczyk M."/>
                <person name="Grzebelus D."/>
                <person name="Spooner D."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="22548759"/>
            <dbReference type="DOI" id="10.1186/1471-2229-12-61"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Located at the top of the head of the small subunit, it contacts several helices of the 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="JQ248574">
        <property type="protein sequence ID" value="AEY81130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093352">
        <property type="protein sequence ID" value="WOH16661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069377.1">
        <property type="nucleotide sequence ID" value="NC_087060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1TIA0"/>
    <dbReference type="STRING" id="79200.I1TIA0"/>
    <dbReference type="GeneID" id="88614164"/>
    <dbReference type="Gramene" id="KZM81289">
        <property type="protein sequence ID" value="KZM81289"/>
        <property type="gene ID" value="DCAR_032430"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614164"/>
    <dbReference type="OMA" id="THTNARN"/>
    <dbReference type="OrthoDB" id="525520at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000011">
        <property type="entry name" value="Ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000003">
        <property type="entry name" value="Ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 12 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEY81130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH16661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="116" mass="13496" checksum="EC81491F8825DEC5" modified="2012-07-11" version="1">MLYISGARSVADKQVRIASTKMYGIGPKKAIQVRYRLGISGNIKIKELTKYQIDQMEQMIGQDHVVHWELKRGERADIERFISISCYRGIRHQDGLPLRGQRTHTNARTCRKQIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X1Z1</accession>
    <name>A0A161X1Z1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0726920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X1Z1"/>
    <dbReference type="Gramene" id="KZM86333">
        <property type="protein sequence ID" value="KZM86333"/>
        <property type="gene ID" value="DCAR_023467"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006527">
        <property type="entry name" value="F-box-assoc_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
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        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="206" mass="24276" checksum="EA3717243618F031" modified="2016-07-06" version="1">MRADSWTTIHCNYFAGVDSIPTVYRVRTPVFLNGSFHWATKRSKWHRTNETDYIVYYKVKDEKVGIMNVFDNYSAIFDIVVDDDERSFDDWKLSVIDERLAMIHWCGERGNLFEVWVMNDYGIDNSWTRLFQIADTLCSYVNPMGYWTNGLMLLDRTACLKRGFDQLQVFFFYDVETRSLKKIPLDHSTGYINGFSSFVETLVPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XIV6</accession>
    <name>A0A161XIV6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0208973</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XIV6"/>
    <dbReference type="Gramene" id="KZN07099">
        <property type="protein sequence ID" value="KZN07099"/>
        <property type="gene ID" value="DCAR_007936"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205854"/>
    <dbReference type="OMA" id="FVFWVNT"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG89735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="49562" checksum="C2B90EEABC419796" modified="2016-07-06" version="1">MDTELPNSSSTATDKQNREQKLDAGSLFVLKSRGSWLHCGYHLTTAIVAPALLSIPFAIGLLGWGGIVCLSMAALVTFYSYNLLSLVLEDHARRGMRQLHFRDMAHDILGPGWGKYFVGPLQFGFCYGAVIACTLLGGQSLKYIYVVSSPDGSLQLYHFIFIFGGVTLIMAQMPSFHSLRHINLVSLLLCLAYCACATAGSIYIGNSSNAPPKNYNVTEVGVNRLFGTFNAISIIATTYGNGIIPEIQATIAAPIQGKMFKGLLICYFVVITTFFSVGISGYWAFGNQAEASVLSNFLVDRKSLVPKWFLLITNIFTLVQVSAVTLTYMQPTNVVIDRRFADPKKDEFSARNIVPRLIFRSIAVIIATTIAAMLPFFGDIMALFGAFGCIPLDFILPMLFYNVTFKPSKRSLIFWVNSIIAVVSTILSGIGAVASVRQIILDANNYRLFADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XQV9</accession>
    <name>A0A161XQV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_017231</name>
        <name evidence="4" type="ORF">DCAR_0519699</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XQV9"/>
    <dbReference type="Gramene" id="KZM93986">
        <property type="protein sequence ID" value="KZM93986"/>
        <property type="gene ID" value="DCAR_017231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036149">
        <property type="entry name" value="APC_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58050">
        <property type="entry name" value="N-terminal coiled coil domain from apc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM93986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH00340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="41504" checksum="6D97FA19B4084E0E" modified="2016-07-06" version="1">MQEVSKVTAPGPDRLSSVQYHAPFPSGGILAHPTMPSESAAGKDRNVHQMLEEGPGDTISAAGSNRSDSVAPGVVAVQYEYEELQHRVHALNNENRFLKEELQKVSEECNKVTSENDSIKGTLMDKEGSVFAALLEEDSLAYSDSFICGSGLLDNTQGQTASSLKMNNGEDTWSLKVAPTDGEKDNSMHNGNSLGSSSLTVGSVVGSSFSGTNFSESSRSCSSGTSYSKNISEVKDGGCVDDWEVLADELAAIDDKEDRSAQKSESLMENRSAVKLDHLPKNTRPSTDDIPKEIVPLSVSCWAEDPADRPEFIEIKYFLEKFIHYLCTPDMSPPRMIKIVHANEVSATGECPCTSQLKQKAKGVGAKPRSAAIRFFRGFRSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XXS3</accession>
    <name>A0A161XXS3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_010211</name>
        <name evidence="8" type="ORF">DCAR_0311541</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XXS3"/>
    <dbReference type="STRING" id="79200.A0A161XXS3"/>
    <dbReference type="Gramene" id="KZN01457">
        <property type="protein sequence ID" value="KZN01457"/>
        <property type="gene ID" value="DCAR_010211"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214988"/>
    <dbReference type="OMA" id="KKNGPCT"/>
    <dbReference type="OrthoDB" id="1912886at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF210">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 86-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="6"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN01457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="241" mass="27720" checksum="7C6E678D36067440" modified="2016-07-06" version="1">MSPVGLPPGFRFHPTDEELVNYYLKRKIHGLEIELDIIPEVDLYKCEPWDLAEKSFLPSRDPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKDRKVSSQNYPIGMKKTLVYYRGRAPQGIRTDWVMHEYRLDDKECDDSTSGIQDSYALCRVFKKNGICGSELEDQGQLNMVLTEEIPQGILTEYETGSPSNILPSAPSACIEDEDKDDSWMQFIKDDAWCSFNTPILGEETSQVTDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y2D7</accession>
    <name>A0A161Y2D7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_012083</name>
        <name evidence="15" type="ORF">DCAR_0313692</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y2D7"/>
    <dbReference type="STRING" id="79200.A0A161Y2D7"/>
    <dbReference type="Gramene" id="KZN03327">
        <property type="protein sequence ID" value="KZN03327"/>
        <property type="gene ID" value="DCAR_012083"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213580"/>
    <dbReference type="OMA" id="WFIAIAD"/>
    <dbReference type="OrthoDB" id="442066at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF36">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007829449" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="25"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="809"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="36"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN03327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG94397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="856" mass="95531" checksum="CD17A7C14871DC49" modified="2016-07-06" version="1">MWSLGQYTIISTLVLLLQPYVAVSSPSGATTHLSPNMQKLALFQIKQSLSINACDHVICVEERLPSYPKTMDWSMNSDCCKWNGVTCNQTTGDVIGLDLSCSCLEGSIPSNSTLFQLSYLQTLNFRANDLNGILPERIFHLPNLKQLNADGNFNLTTILPKINWGSRSSLQMLSLQETTISGGIPHSVGYLKSVTIFSLSSCKITGLIPKFIGNLAQLTELNLNYNNFSGPIPDSFANLQNLKYLSLRDNKISGQFPTWVASLTQLKFLDLSGNFLSRPLPSNLTARCLPKLMLLNLSYNLFNGTIPSWLFDLPSITRLFVDHNGFTGQLNEFKSSTLLTFNCANNLLSGTIPQSLSKLASLISFDISYNNFSGVVKITTLNPNIVDLGLASCKIMKIPHYIRSLEKLRYLDLSNNQIGGEIPQWIWKGSLNQLNLSYNYFNGGLQHLPENRLIFLNLQHNKLKGSLPASICNSSSLEVLNLSNNNLSGVLPVCPRSLNYSLAVLDLRMNRIRGSLPSSLSNFRELKSLNLYGNEVEGTIPLSFAKFDNLEVLDLGSNHLKDTFPQWLEALPNLQVLILKSNKFHSTINSLSKIQHPFPSLRIIDLSNNEFSGPLPAKYIENFKGMMNGDANKIESIYMGNSYYRDTVKMVIKGVELEFVRILTVFTTINLSGNSFEGDIPESIGNLKSLRHLNLSHNHLSGHISSLIGELFMLESLDLSFNRLVGVIPQELAGIYTLSRLNLSHNDLNGHIPVGTQFQTFENDSYVGNLALCGPPLSKKCKREFIEMQDEEDEDADYFFSGFTWEAVVIGYGSGVVVGFAVGYIMFRTRDPKWITGIIIKKIGLKVRRSEIRRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y398</accession>
    <name>A0A161Y398_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_012423</name>
        <name evidence="12" type="ORF">DCAR_0314073</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y398"/>
    <dbReference type="SMR" id="A0A161Y398"/>
    <dbReference type="Gramene" id="KZN03667">
        <property type="protein sequence ID" value="KZN03667"/>
        <property type="gene ID" value="DCAR_012423"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215339"/>
    <dbReference type="OMA" id="DYYIATP"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF157">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="12"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y8M1"/>
    <dbReference type="Gramene" id="KZM88789">
        <property type="protein sequence ID" value="KZM88789"/>
        <property type="gene ID" value="DCAR_025864"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196459"/>
    <dbReference type="OMA" id="CQFANVQ"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000005">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH10249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="519" mass="57232" checksum="E7D32521959B29BB" modified="2016-07-06" version="1">MAMALALRKLSSSLADKPARRLVNGGSLYSMSSLPSEAVYDKEHPGVTWPKQLNASIEVVDPEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAERLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYDRIRKVCDKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRKGLKEVTKQGKEVMYDFEDKINMAVFPGLQGGPHNHTITGLAVALKQATTGEYKAYQEQVLSNCSKFAQTLMKRGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEFFDSAVKLALKIKSEAKGTKLKDFVAAMESSAPIQSEISKLRHDVEEYAKQFPTIGFEKESMKYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AHL2</accession>
    <name>A0A162AHL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_009765</name>
        <name evidence="6" type="ORF">DCAR_0311025</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AHL2"/>
    <dbReference type="STRING" id="79200.A0A162AHL2"/>
    <dbReference type="Gramene" id="KZN01011">
        <property type="protein sequence ID" value="KZN01011"/>
        <property type="gene ID" value="DCAR_009765"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211157"/>
    <dbReference type="OMA" id="NDQIMIR"/>
    <dbReference type="OrthoDB" id="1435597at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000086">
        <property type="entry name" value="Transcription factor VIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052483">
        <property type="entry name" value="bZIP_transcription_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391:SF20">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="176"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN01011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG91774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="35796" checksum="ED2E90B37E34681B" modified="2016-07-06" version="1">MAQLPPKVPNMAPNLAGLSSYSHHQKLPSMENLNSLQSHPWVDDYLNFSSSTKRRSHRRSASDSIAFLEQVSVDHEEECKVRPLAPGFRTSHAAAAQEFDQFDEEQFMAMFTDDEAVDLTVICSSPSSPSHHNCNIEDDQQMQLGNGFRSGSTLDGQNAAGDGVATIDNYNDRNFDSRRVKRILANRQSAQRSRVRKLQYISELEHGVNSLQAEISVLSPRVAFLDHQRLVLNVDNSVLRQRIAALSQDKIFKDGHQEALKMEIERLRQVFNYQQSVKKTDDDDTTLPLAESLVQTSPEKVTKHPNCSSFNDQIMIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AVZ5</accession>
    <name>A0A162AVZ5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0208431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
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            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="WOH10697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="119" mass="12379" checksum="AE1792113661B627" modified="2016-07-06" version="1">MHSVAGISFAAVICYFGEKVLGRNKPDTRQQVIVSCAAAGVSFVAAICYFGEIVVGRNKPDVSFEIQQTKLEVQALKQQVDRLVVMFDASPPASPSFTAITPASPAAAPPSAATAAPPS</sequence>
</entry>
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        </lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="protein sequence ID" value="WOH10853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_026416"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-EC"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="79"/>
            <end position="360"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W8Z8</accession>
    <name>A0A164W8Z8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="5 6">
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin U/P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W8Z8"/>
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        <property type="protein sequence ID" value="KZM91447"/>
        <property type="gene ID" value="DCAR_021188"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227758"/>
    <dbReference type="OMA" id="QVFETYC"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Cyclin_P/U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF121">
        <property type="entry name" value="CYCLIN-U1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="215" mass="24521" checksum="3D0EEB5D4C1389FA" modified="2016-07-06" version="1">MLASDEDSQQHSRLEPSSTPRVLTILAFVLEKLVVRNDRLVDGVSREQGKSLDVFHGARPPNISIIKYMERIYKYTNCSPSCFVVGYVYIDRLLHKYPHSLVVSLNVHRLLVTAVMLAAKMLDDVHYNNAFYARVGGVSNVELNRLEIDLLFLLNFEMTVHSRVFESYCQQLEKEMLLNSTTLKIERPMISNAIDDVTEISMEDMPSSSPPQLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WWH5</accession>
    <name>A0A164WWH5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0626156</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for integral membrane and secreted preprotein translocation across the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with OEP61/TPR7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WWH5"/>
    <dbReference type="Gramene" id="KZM92361">
        <property type="protein sequence ID" value="KZM92361"/>
        <property type="gene ID" value="DCAR_020274"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227899"/>
    <dbReference type="OMA" id="KSINCQC"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000038">
        <property type="entry name" value="DnaJ protein ERDJ2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000007">
        <property type="entry name" value="DnaJ protein ERDJ2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="615"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH06728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="682" mass="76289" checksum="CE304D2A24A4D578" modified="2016-07-06" version="1">MATSDENSGLFPIFILTILAIPLVPYTILKLCRAASKKAKPIHCDCSVCSRSGKYRKSIFKRISNLSTCSNMTLVLLWVVMGVLVYYIKHISREIQVFDPFTILGLESGASDSEIKKAYRKLSILYHPDKNPDPDANKYFVESISKAYQALTDPVSRENFEKYGHPDGRQGFQMGIALPQFLLNFDGASGGILLLWIVGVCILLPLVIAVVYLSRSAKYTGNYVMHQTLSAYYYFMKPSLAPSKVMDVFIKAAEYMEIPVRRSDAEPLQKLFVVVRSELNLDLKNIKQEQAKFWKQHPALVKTELLIQSHLTRDTAALSPELQRDFKRVLELAPRLLEELMKMAVIPRTAQGHGWLRPAVGVVELSQCIIQAVPLSAKKAGGGSPEGNAPFLQLPHFSEAVIKKIARKKVRSFQDLRDMTLQDRDELLSQVAGFTAADVQDVERVLEMMPSLTVDITCETEGEEGIQEGDVVTVQAWVTVKRGNGLIGALPHAPYYPFHKEENYWFLLADPNLNNVWFSQKVIFMDEAAAITTASKAIEEMMEGSGASPKETSKAIREAVEKVKAGSRLVIGKFQAPAEGNYNLTSYLLCDSWLGCDKKTNVKVKVLKRTRAGTRGAHITEEGPITEDGAEEEEENEEEEYDEDYESEYSEDEEDARETNNKGTADQNGRHSNSESSESEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XVY7</accession>
    <name>A0A164XVY7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_016891</name>
        <name evidence="3" type="ORF">DCAR_0519316</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XVY7"/>
    <dbReference type="STRING" id="79200.A0A164XVY7"/>
    <dbReference type="Gramene" id="KZM93646">
        <property type="protein sequence ID" value="KZM93646"/>
        <property type="gene ID" value="DCAR_016891"/>
    </dbReference>
    <dbReference type="OMA" id="QVEERQC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009638">
        <property type="term" value="P:phototropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039615">
        <property type="entry name" value="PKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33781">
        <property type="entry name" value="PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM93646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="275" mass="31067" checksum="CB05B6416DA354DA" modified="2016-07-06" version="1">METNASSQVGLFDAGRILIQESWSPERNQRCSSSSSVGNYSTPSLNGTPSNASSEASSNSHKGLIMNRPKPVSIHLSDRKQGKGRRKSTASDSIKWLFRQRCLCSGKKCVQVKQEVLVLSHNKLKFPPLSADEMYPRNQIVDHKMTQRAKQRAQIIVQNSTRQRLISLPIFSNPATTDDDVASNASSDLFEIQSFSTHTTYFPPDESQTDMIYYEQMTPSIEYCYYEPSEADIGWSITTSEGFDLDRPSMSTFHISDHQVEERQCQSDYVSLRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XYZ7</accession>
    <name>A0A164XYZ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Target of rapamycin complex subunit LST8</fullName>
            <shortName evidence="5">TORC subunit LST8</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Lethal with SEC13 protein 8 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_016999</name>
        <name evidence="7" type="ORF">DCAR_0519454</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of TORC1 complex, which is an essential cell growth regulator that controls plant development. Acts by activating transcription, protein synthesis and ribosome biogenesis, and inhibiting mRNA degradation and autophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The target of rapamycin complex 1 (TORC1) is composed of at least RAPTOR, LST8 and TOR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XYZ7"/>
    <dbReference type="STRING" id="79200.A0A164XYZ7"/>
    <dbReference type="Gramene" id="KZM93754">
        <property type="protein sequence ID" value="KZM93754"/>
        <property type="gene ID" value="DCAR_016999"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220109"/>
    <dbReference type="OMA" id="VQRNYKH"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000179">
        <property type="entry name" value="Target of rapamycin complex subunit LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="208"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="254"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM93754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH00097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="316" mass="35381" checksum="605825D163F8EE5A" modified="2016-07-06" version="1">MTQQPSVILATASYDHTIRFWEAKSGRCYRTIQYPESQVNRLEITPDKRYLAAAGNPHIRLFDVNSNSPQPVMSYDSHTNNVMAVGFQCDGNWMYSGSEDGTVRIWDLRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNRGTCYVWRLLKGTQTMTNFEPLHKLQAHDGYILKCLLSPEFCEPQRYLATASSDNTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDATARLWSVSTGEDMRVYQGHHKATVCCALHDGADTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z8C4</accession>
    <name>A0A164Z8C4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_018750</name>
        <name evidence="13" type="ORF">DCAR_0521452</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-apiose + CO2</text>
            <dbReference type="Rhea" id="RHEA:70523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:73883"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
            <dbReference type="Rhea" id="RHEA:23916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="4.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23917"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z8C4"/>
    <dbReference type="STRING" id="79200.A0A164Z8C4"/>
    <dbReference type="Gramene" id="KZM95508">
        <property type="protein sequence ID" value="KZM95508"/>
        <property type="gene ID" value="DCAR_018750"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223530"/>
    <dbReference type="OMA" id="WIYSCAK"/>
    <dbReference type="OrthoDB" id="331544at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102765">
        <property type="term" value="F:UDP-D-apiose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033352">
        <property type="term" value="P:UDP-D-apiose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05257">
        <property type="entry name" value="Arna_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000201">
        <property type="entry name" value="UDP-D-apiose/UDP-D-xylose synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045869">
        <property type="entry name" value="Arna-like_SDR_e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF13">
        <property type="entry name" value="UDP-D-APIOSE_UDP-D-XYLOSE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM95508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH02064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="389" mass="43563" checksum="3953523BC4B8E5D4" modified="2016-07-06" version="1">MASAAARVDLDGKPITGITICMIGAGGFIGSHLCEKLMSETSHKVLAVDVYNDKIKHLLEPDSLPWNGRIQFHRINIKNDSRLEGLIKMADLTINLAAICTPADYNTRPLDTIYSNFIDALPVVKYCSENNKRLIHFSTCEVYGKTIGSYLPKDSPLRQDPAYYILKEDESPCIFGSIEKQRWSYACAKQLIERLIYAEGAENGLEFTIVRPFNWIGPRMDFIPGIDGPSEGVPRVLACFSNNLLRREPLKLVDGGESQRTFIYIKDAIEAVMLMIENPARANGHIFNVGNPNNEVTVRQLAEMMTKVYSKVSGEPSIDSPTIDVSSKEFYGEGYDDSDKRIPDMTIINKQLGWNPKTSLWDLLESTLTYQHRTYAEAIKQAIAKPIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A2D4</accession>
    <name>A0A165A2D4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_015840</name>
        <name evidence="10" type="ORF">DCAR_0414859</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A2D4"/>
    <dbReference type="STRING" id="79200.A0A165A2D4"/>
    <dbReference type="Gramene" id="KZM96798">
        <property type="protein sequence ID" value="KZM96798"/>
        <property type="gene ID" value="DCAR_015840"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217848"/>
    <dbReference type="OMA" id="MNPNNAT"/>
    <dbReference type="OrthoDB" id="207175at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF287">
        <property type="entry name" value="DEVELOPMENT RELATED ERF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="94"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="165"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM96798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG95535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="211" mass="23722" checksum="8C595140BE24FA5E" modified="2016-07-06" version="1">MNREMISTNDLPCLLQSIEHHLFDDSELSDIFPTMNLWDVPDTSLTTLFFMDNTSDQTLPMDCYQPDHVDAREETKEKEKEVSARAHPDHIRFRYRGIRRRPWGKFAAEIRSPAKKGKRVWLGTYETPEDAALAYDRAAFKIHGSAAKLNFPHLIGSNLPDPVKVAPRQRTSSVPSLTSSSSSLSLLSSSSSNNGSFKKREKNAAKAIKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XFD6</accession>
    <name>A0A165XFD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ornithine cyclodeaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_014522</name>
        <name evidence="3" type="ORF">DCAR_0416399</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XFD6"/>
    <dbReference type="STRING" id="79200.A0A165XFD6"/>
    <dbReference type="Gramene" id="KZM98116">
        <property type="protein sequence ID" value="KZM98116"/>
        <property type="gene ID" value="DCAR_014522"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216531"/>
    <dbReference type="OMA" id="VKIVNVH"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062046">
        <property type="term" value="F:dehydropipecolic acid reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062034">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <source>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
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        <subcellularLocation>
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    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        </location>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    </evidence>
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</entry>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A5F5"/>
    <dbReference type="Gramene" id="KZN00762">
        <property type="protein sequence ID" value="KZN00762"/>
        <property type="gene ID" value="DCAR_009516"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210585"/>
    <dbReference type="OMA" id="PMIVFRD"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG91503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="768" mass="88070" checksum="3E3DA9CB3B8442BF" modified="2016-07-06" version="1">MGAPSSDTQTVSNDTVVNIMGSDLVLNSMQPDLSYDPHFVTDCPPAHRSSWSEIPRSPNLYGQSSPAKFSFSGENMRHRRRQSGSGFSSNKSLPRHSQLMRMKSKSRLIDPPEQYDNSERIMKSECMEARSECQYDDPYAKEDLPEEFKMIKFSKWAVIQFFCMIVIIPALVCSIFVNSSHKNILCGLELWRWGILLLALLCGRLVAGWGLRVVVFLVELNCMLRKRVLYFVYGLRRAVNNCLWLALILIAWRIVLFDRVEHLAHGKVLSYVTKAWVCLLVGTLIWFVKTLLVKVLASSFHVSTFFDRIQCALFDQYVIETLSGPPLIGNHLEPVEEEQSTAEVPQLQDAAGNTVSPRTTASPRSGKLKTITKRRKIFPSFKSSRFSTVAAKEEKEGITIGHLHRLNGKNISAWNMKRLMNIVRKGNLSIDEQICEDETAVQIKSETQAKNAAKKIFCNVAKQGSKHIYQDDLMQFMRDDEALTTISHFEGASGWKGISRKELKNWMVNTFKERRALVLSLNDTKKAVNKLHQMLNIFVGLIILIIWSLILKVASTQFFVLLSSQFLLVVFVFGNTCKTMFEGIIFLFVMHPFDIGDRCEVDDVQLVVEEMNILTTTFLRYDNQKICYPNSVLSNKPISNYYRSPDMGDAIDFCINISTPVEKIALMKEKITSYIEKNSDHWYPAPMIVLRDIEDMNRLKISLWLSHRINFQDMGERWVRRALIVEEMIKTFKELDIEYRMPVVDMNVRSMPAIVSNRLPSIWTSHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AVU6</accession>
    <name>A0A166AVU6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_010753</name>
        <name evidence="3" type="ORF">DCAR_0205649</name>
        <name evidence="4" type="ORF">DCAR_0934238</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG86444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH14716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_031806"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_030154"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_015985"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN01999">
        <property type="protein sequence ID" value="KZN01999"/>
        <property type="gene ID" value="DCAR_010753"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN01999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="52" mass="5825" checksum="556638D95690CCCF" modified="2016-07-06" version="1">MEISSNQTVFCSNQAQFSSNQANFREVADIQSSSDTDELLPVPDDYSHQELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B442</accession>
    <name>A0A166B442_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0312580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B442"/>
    <dbReference type="Gramene" id="KZN02334">
        <property type="protein sequence ID" value="KZN02334"/>
        <property type="gene ID" value="DCAR_011088"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214289"/>
    <dbReference type="OMA" id="CANAPRW"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620697" description="Expansin" evidence="7">
        <location>
            <begin position="23"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG93299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="26360" checksum="C3C4EFBFD94AF304" modified="2016-07-06" version="1">MDTKITGIVVLGFLSIVSSVQGYNRGWINAHATFYGGGDASGTMGGACGYGNLYGQGYGTNTAALSTALFNNGLSCGACFQIVCVNDPKWCLRGAIIVTATNFCPPGGWCDPPNHHFDLSQPVFLRIAQYRAGIVPVAYRRVPCRRRGGIRFTINGHSYFNLVLVTNVEGAGDVHAVYIKGSRTRWQPMSRNWGQNWQSNNYLNGQTLSFKVVTSDGRSVVSYNVAPSGWSFGQTYTGRQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B9B1</accession>
    <name>A0A166B9B1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0312744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B9B1"/>
    <dbReference type="Gramene" id="KZN02520">
        <property type="protein sequence ID" value="KZN02520"/>
        <property type="gene ID" value="DCAR_011274"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214354"/>
    <dbReference type="OMA" id="GNNTDMG"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG93460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="369" mass="42416" checksum="EA6542AD37601AA2" modified="2016-07-06" version="1">MGGMTSSMAAKFAFFPPNPPSYKLVTDNLTQLLLISPFPHRENVEILKLPTRRGTEIVGMYVRHPMATSTLLYSHGNAADLGQMYELFIQLSIHLRVNLFGYDYSGYGQSSGKPSEQNTYADIEAAYKCLEESFGTKQEDIILYGQSVGSGPTLDLATRLPKLRAVILHSPILSGLRVMYPVKRTYWFDIYKNIDKIPLVHCPILVIHGTADDVVDFSHGKQLWEMCNEKYEPLWLKGGNHCDLELFPEYTRHLKKFVSVVEKSPPQGHSSRRSTDHFEQSRKSTDVYETSRKSTDRREKPRHSTDRHEKLKIPSGNNVDKLEKIRMSFDQMERSRRSVDCFDKSRKNMDHQLDRGRKSVDQLERLRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C8X9</accession>
    <name>A0A166C8X9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0313828</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Secreted</location>
            <location evidence="2 11">Extracellular space</location>
            <location evidence="2 11">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166C8X9"/>
    <dbReference type="Gramene" id="KZN03450">
        <property type="protein sequence ID" value="KZN03450"/>
        <property type="gene ID" value="DCAR_012206"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212809"/>
    <dbReference type="OMA" id="TICKRSV"/>
    <dbReference type="OrthoDB" id="1546383at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620701" description="Germin-like protein" evidence="11">
        <location>
            <begin position="26"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG94532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="22594" checksum="513693CEB276ED1C" modified="2016-07-06" version="1">MAWLLVDHALLFSSLIFAFLRMTQSTDPDILSDFVIPANATTIDENFFTYTGLRTAFTAEFPPDFKALKANMVEFPGLVGQSISYLVVQLPIGSVNPPHIHPRSSELLFVISGYIEAGFVDTSNKLYTQTLQPGDIFVVPKGLLHYQYNANASFNATALTAFGSANGGKVTLPVALFETGIDDLILAKSFKTDIATVQKIKAGLAPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CFT7</accession>
    <name>A0A166CFT7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0314135</name>
    </gene>
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    <sequence length="1099" mass="120960" checksum="BAA8E5D8CD7C6E0B" modified="2016-07-06" version="1">MFGAAGKSGRGGRGGTGKRKLPSKFRSASRNLPPSGRPSVDASRNNRTTTATTAVASTAPAEETYDLVTGNPLNFAMIIRLTPDLVDEIKRAESQAGSARIKFHANPNYPSGNVIDVGGKEFRFTWSREMGDLCDIYEECQSGEYGNGQLVESGCAWRKLNVQRVLDESTKNHVKMMSEEAERKSKSRKVTILDHGSSSMKTQIKALAAAEATPWKMPFKQKKEPPYKKGTTEPPSGPPKSANKHVLSSTPSKGRISSSPLPSAREQPLVSASPSRTRSLSRAYASIDDNMSIQASSKEKPSSSEKGMPNRVITNAVPDKQAGKGNLGVKPTDLRSMLIVLLRENPRGMSLKALEKSVGQDFPNSGAQIKLIIKKVATYQPPGRYILKSGAEPESFQKPVSQSGSSPENKKQEASRRSDKCDDIHDPVNKASMKTHTQNDEPANLNSEPGEVLTDAEKHKISLQSPDLHAEKEVLDNSGRMVATSSNSQSGSDSSDSECDSGSHSRSRSKSRSLLGSGSGSSSDSETDTSSKSKEGSDEEVDIMTSDDDNVLKDRLQASEPELSASPVLQRPEGLLLQNATNEKKDIHAAEVIEIRENSHGCAQVAKFDAYFLGSVSSDKEGEQHSQVIKPLSDYHAVPQETQVPRGDLYREKDGFRLEQSDGYQRKSEGKSKKRSAEDLFDDTDVCSKKLKGSATRAPIQSEDGYKNEKRPSKNPRDGAVDKKMAPADSHHRKKAELPGKTVEVGSVLNSSLVYPLKDSNISDVGRSAIIDILNLRRESSVLELGELRDPLPEMTPGIKQSDKIGSINQTVRQLDYWNPDISKERPAGKTGVDSVEPSSANLNAGVIGNYKGLYKSGSQGHDEDLSAVQCNPPQLRRVNQAEVVSQLTNLSDVDSNCKQNKALSSQVVGREGYGDAQRKVPGAMAQLLDDVQGRPLSTIESERRKTDSSANLSDIYNDPPILDSRAGGKENRGTFFNTNDLSYYKYEKDKPELKGPIKDQSQYMEYVKEFQEKYDSYFSISKILESERNEFLKFGRDLEAAKENDMIKYCSILEKLRESYRRCGTRHKRLKKVYVVLHRELETLKEMIRDYANRCPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FAM5</accession>
    <name>A0A166FAM5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0209484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FAM5"/>
    <dbReference type="Gramene" id="KZN07557">
        <property type="protein sequence ID" value="KZN07557"/>
        <property type="gene ID" value="DCAR_008394"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="351" mass="39064" checksum="BFE7011285FBCB9D" modified="2016-07-06" version="1">MVLPGSNRKSSALSIEANINESVQAVPAIVRDENKCKNIIKHTTRKSSFKREGKKEIECSTKRISDAVEDFKKFSDSLMEELEADYQQMFEQMKKELENLAALPNSTMQTIDNEGEGIQNSNHVPELTRTHSEPQEQQNRSSSSNFITPAGHQLVPYTGFSQGSGSLTTTAVNLPMIELQEQQNRSSSTNFITPAGHQLVPYTGFSQGRGSFTTTAVNRPIIGQFTTNTRLIPGTRVSSLTTAVNRPAGYFTNLLRQVTNPGNLSYPHGNNAQAFNRAGSTDQFPLPPARRLGDASSFLNRNMENTSFGSSHALTYDSVPANRFRDINFFENFEMNESWLRRHGRRNGDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FGN5</accession>
    <name>A0A166FGN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_008601</name>
        <name evidence="24" type="ORF">DCAR_0209718</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KEA (TC 2.A.37.1) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FGN5"/>
    <dbReference type="Gramene" id="KZN07764">
        <property type="protein sequence ID" value="KZN07764"/>
        <property type="gene ID" value="DCAR_008601"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208738"/>
    <dbReference type="OMA" id="ARIPFRF"/>
    <dbReference type="OrthoDB" id="4834at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140899">
        <property type="term" value="P:plastid gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042794">
        <property type="term" value="P:plastid rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080022">
        <property type="term" value="P:primary root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001057">
        <property type="term" value="P:reactive nitrogen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900069">
        <property type="term" value="P:regulation of cellular hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010109">
        <property type="term" value="P:regulation of photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000070">
        <property type="term" value="P:regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900140">
        <property type="term" value="P:regulation of seedling development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000007">
        <property type="entry name" value="K(+) efflux antiporter 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000134">
        <property type="entry name" value="K(+) efflux antiporter 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004771">
        <property type="entry name" value="K/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00932">
        <property type="entry name" value="2a37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157:SF2">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1001">Plastid inner membrane</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="698"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="727"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="760"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="843"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="884"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="915"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="939"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="975"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="1023"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="376"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="273"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KZN07764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG90474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1221" mass="131166" checksum="7A06DFF703688F6D" modified="2016-07-06" version="1">MDCICSFQQLSVISGGEGSNYSLLESLNSNLSFKCRSIGSKRLVLAGRSEKLKKLVTCCRNEVVSYLGCHMLPRSSGFSFTCGIESSSGVVKTCCQGEDSVVFINGNGNGRRVDYGASANGEGLTDGVDDSSEGDGVVEEVEKVEGTDILSLEGLRGLLQKALKELEIAQLNSRMFEEKAERISEAAIALKDEADSAWKDVKYTLAAIREIAKREGRAKEVVQTATMSLSLAMARLQVAGESLESAKRKTSYKDKSTEGYADYDNVGDKSNALREEEETILSAQEDIRECQARLAKCEMELRQIQSKKQELQKEVGRLVGVAEKAEMDASKAEEDVANIMLLAEKAVAFELEAAKQVNDAEIILQRAEKTLSVSPVTSSKWVLQQNESSKVSESTLVEEDKMSLENSGDSNVDRDKKVPQDTSSLIGDASSDNQLDVQGQRLEDSSESGDGVLENGKLSTSQKEIEEEAEKSENVVHNRKSESQKDSNRDSSPHSAPKALLNKSSRFFPASFFSFAGDGTEFTPTSFFYGLIKSGREQLPKLVVGLLLAGSGIAFYATRTEKVNHIFQQSDIITASIDEVSSNAKPILRQVLKLPKKLKLLMDKIPHQEVNEEEASLFDMLWLLLASVIFVPLFQKIPGGSPVLGYLTAGILIGPYGCSIIRNVHGTKAIAEFGVVFLMFNIGLELSVERLSSMKKYVFGLGSGQVLVTAVVVGMIAHIVAGQAVPAALVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGVGFQAIAEALGMAGLKAVIAISAIVAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIMGTLGLLIGGKTILVALIGKLFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQFIASRFEQHDVRSLLPVESETDDLQDHIILCGFGRVGQIIAQLLSERLIPFVALDVKSDRVSVGRALDLPVYFGDAGSKEVLHKVGAERACAAAITMDTPGANYRTVWALNKYYPNVKTFVRAHDVAHGINLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEITATINEFRSRHLSELTELCEASGSSLGYGYSRMMTKPKSMPLDLSDEDELPEGTLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G4N4</accession>
    <name>A0A166G4N4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_001059</name>
        <name evidence="3" type="ORF">DCAR_0101085</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166G4N4"/>
    <dbReference type="Gramene" id="KZN08529">
        <property type="protein sequence ID" value="KZN08529"/>
        <property type="gene ID" value="DCAR_001059"/>
    </dbReference>
    <dbReference type="OMA" id="NITRGCE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21070" checksum="FF80FFD593092CA2" modified="2016-07-06" version="1">MHFVAMDEQSVQQLTIKAVLELQIPAQMRCLCKAEVVEILNGDGWYYECCATCARAVQKKGRKNILPRLPGGQKKVSHRVVARVKDESGTTTFTIFNKEAELLIGVPLEKIFSELAPINTMEGTIKDIPTPIKNMMGKVCVFQIKVTEYNITRGCEEYTVTRVSECSSPVASKTIGEEGSHKDKRMKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GCU7</accession>
    <name>A0A166GCU7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0101422</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GCU7"/>
    <dbReference type="Gramene" id="KZN08818">
        <property type="protein sequence ID" value="KZN08818"/>
        <property type="gene ID" value="DCAR_001474"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196138"/>
    <dbReference type="OMA" id="VRSAKMD"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="205" mass="23477" checksum="812221FFCC31953C" modified="2016-07-06" version="1">MVRGKIQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYDLATKGTMQGLVNKYLKSTRGADPDVEDCQATEKQVQDTKMEIDVLKTEIDLLQKGLGYMSGGGTGKMTMDELHMLEKNLQVWIDHIRSVKMDIMFQEIQLLKNKEEILRAANHYLSDKMSNQYGFINIDTPVMDNIAYPLTIQNEIYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IT11</accession>
    <name>A0A166IT11_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Grzebelus E."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <tissue evidence="1">Leaf</tissue>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KZM83098.1"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    <sequence length="77" mass="8400" checksum="E0100A0C2EA66B38" modified="2016-09-07" version="1">MGLLDLRYEGIGGARIKCIEGHHNPNHIVQLGAPVNNTCETHKNIVVFLKVGGARAPWCPNVAPPLYEGDKVKQMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YJP7</accession>
    <name>A0A175YJP7_DAUCS</name>
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        <alternativeName>
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    </protein>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    </reference>
    <comment type="function">
        <text evidence="3">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
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    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
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            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; glutathione metabolism.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="429"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="481"/>
            <end position="482"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH11220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="627" mass="67621" checksum="17A28F626A54ECC8" modified="2016-09-07" version="1">MKQLRLEDPLLVTSGNSSTTRWLRALCFFLALTAITFAGLTLSGYLEFGIVRGGGNYGNEGLSFGNEEVVKSDKAVVAADDARCSETGVSVLGQGGHAVDAAVATALCLGVVNAMASGIGGGGFMVVRSSATSRTEAINMRETAPLASSQTMYDNNPETKYKGALSMGVPGELAGLYEAWSRHGRLPWKTLFQPAIKLARDGFVIAPYLGNYIADYSDSIMRDPGLRQVYAPGGKLLKAGEKCYNVELGHSLEAIGELGPKAFYDGSIGERFVQDVRKAGGILTMEDLRSYRVDVTEAVSVNAMGYTILGMPSPSSGTLGISLVLNILESYGSTYAAMGSLGLHRLIEALKHMFAVRMDLGDPNFVNITKTVADMLSPAFAKRIQQRIFDNTTFPSEYYLHRWSQLRDHGTSHFCIVDADRNAVSLTTTVNYPFGAKVLSPSTGIVLNNEMDDFSTPAEVSPDQLPPAPANFIEPKKRPLSSMTPVVVLRDNQLVGVIGGSGGLYIIPAILQVFLNHFALGMEPLSAVQGPRVYHKLIPNVVLYEDWTCLDGEHIEVVPEVRRFLEERGHELEAKSGGAISQLIVQDLRNAIPMDRENGKGTSDQVLCGILTGVSDPRKDGKPAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKP3</accession>
    <name>A0A175YKP3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Pectinesterase</fullName>
            <ecNumber evidence="5 15">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_028535</name>
        <name evidence="19" type="ORF">DCAR_0830797</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Secreted</location>
            <location evidence="1 15">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="5 15"/>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKP3"/>
    <dbReference type="Gramene" id="KZM84043">
        <property type="protein sequence ID" value="KZM84043"/>
        <property type="gene ID" value="DCAR_028535"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198067"/>
    <dbReference type="OMA" id="IRAICNV"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018040">
        <property type="entry name" value="Pectinesterase_Tyr_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00800">
        <property type="entry name" value="PECTINESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6 15" id="KW-0134">Cell wall</keyword>
    <keyword evidence="11 15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="17">
        <location>
            <begin position="63"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KZM84043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH11316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="583" mass="64941" checksum="4FB5B09ACC0411D7" modified="2016-09-07" version="1">MSSIDMFKRELEEHALRKKARKSLIIISVSVILLVIIIIAALVGGLKRNDNSNKSPPSSSSTPTTESIKAICEVTLYPDSCSSSISSLKSATNSTSNNPEDLFVLSLQAAVNALVEFSSFTQMLADSNSINSPLSKNALSSCESIISDAIDYVNMSVVRFAETENKLSPSTSTINDIRTWLSAAITYQETCIDGLQEFSQGNMKLTEEVRTAMRNSTEFTSNSLAIVTKILSKLRFPMNRKLLNSEVDGSPSWFYPNRRLQDIEKNLPKPNVTVAKDGSGDFETIGEAVKNIPERSRYRFVIYIKEGVYVENATIGKRQWNVTMYGDGIGKTIITNNLNVVDLTPTFISGTLIAEGRGFVAMHMTFRNTAGPEKGQAVAMRSSSDHSIFYKCSFEAYQDTLYVHANRQYYRDCQITGTVDFIFGNAAVVLQNCTIQPREPLPKQFIAITAQSKSDPNQNTGICIQGCDLSTTFNNQNVTADTYLGRPWKDYSTTVVMHSKIGPLLNPQGWTEWVRDITPPETIFYGEYQNSGLGSLLDQRVEWDGYKRNLTDQQASKFTVEGLIQGSEWLVDHARLYNYDLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YLG7</accession>
    <name>A0A175YLG7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_028600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH00640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="349" mass="38654" checksum="0C7985A2714345EF" modified="2016-09-07" version="1">MRHLALQVRTKLAENDFKLKAYKNSSQIVQDISEKGSKNNKLGIGYEYGPSKESVSDYVDNDTAKPLVLRKVQKPVFKFVESVFDEEALLIKQELNDEDSVSNSVTVNEKNTVKRPMTVDSIYSQMAHSKLAINKAETELSKGVVKRKPKIVTFITTVKTVEANAAVKGDPTATFECAVNTDDTKSRNRNGKINRGKNGEYVKSAPRKLCNNRGSSHHLTNVCKNDVVTPINAVKINGNLHRTPIIDRSMNVCSDIDCMPCKITAMSTVFNPPILSTTKCSYLCDVETPEPTNASSKATPTKKKVVPFSKSLWVKRDVKNLVVHDLDVVDETLKFEPNDDATSSNDVTA</sequence>
</entry>
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    <name>A0A175YLS9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_028477</name>
        <name evidence="12" type="ORF">DCAR_0830825</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH11343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_028477"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199012"/>
    <dbReference type="OrthoDB" id="1918918at2759"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
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    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF215">
        <property type="entry name" value="OS02G0781300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
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    </dbReference>
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        <property type="entry name" value="ETHRSPELEMNT"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="129"/>
        </location>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM84101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH11343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="196" mass="21735" checksum="C8BE111A7D0A4165" modified="2016-09-07" version="1">MDINVTRESSGQAGHDQQVKYTGVRLRKWGKYVSEIRLPNSRERIWLGSYDSAVEAARAFDAAQFCLRGPSAKFNFPDNPPDIPGGRSLTPGQIQVEAARFAHSAPPQCSANNPQAEAHQAEAPSNTGQVGVENSLLDEFRTMSWETESVSEFGYFQGFDDFENELLLSGPNYGMDEVEETGDDSFTTHGSFLWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="6 7">
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH13254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPU7"/>
    <dbReference type="Gramene" id="KZM85754">
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        <property type="gene ID" value="DCAR_026824"/>
    </dbReference>
    <dbReference type="OMA" id="EIYWGLR"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    <dbReference type="InterPro" id="IPR058054">
        <property type="entry name" value="Znf_MS1-like"/>
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    <dbReference type="InterPro" id="IPR001965">
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    <dbReference type="InterPro" id="IPR019787">
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR46201">
        <property type="entry name" value="PHD FINGER PROTEIN MALE MEIOCYTE DEATH 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46201:SF1">
        <property type="entry name" value="PHD FINGER PROTEIN MALE STERILITY 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00628">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="672" mass="76012" checksum="26455A560402A4AC" modified="2016-09-07" version="1">MSGLEATIGCKKRKRSGERVYKFKTFGEQGCPAEFNHGCFRENVRALLEFGNSETVSWGGMASWSFQLQVQRNPPSHILLFVIEEPVELSLNTHCKHCLYVGWGEHIICNKKYHFLVPSKDTLAACLSYEGNNMNSFGAGTDRVKGKLSLIMELQGHILHGVFHSNGFGHLLCINGLEMGSDLPGYQVMDFWDRLCTALQARKVSIKDTSQKTTMDLRLLYGIAYSEPWFARWGYEFGRGSFGVTKQMHQKAIEAIQTIPLCVLAHHATSNNSNHEISIMVSKYQTVAGHSMKSLSDLFRFILELKSRLSKESSIDTCNPGILVDTNCRWSIKRVEMAIRVIIDSLKRAEFRWISRQEVRDSARAYIGDTGLLDFVLKSLGNHIVGNYLVRRCLNPVTKVLEYCLEDISNVNFSHQDQEFSSDDSISVKARYKISRIQLSKDLFYLYKHIFKEQNSSTNSGITATISLASRIILDSKYLIKEYFKKLPYKFDTGLGDRSKLYCTIALTSLSGVEPKKVAAPYECITLGNNATFQDLTLEVEKCFKDIYWGLRDFAVQTITNMNALNVLKRSDDLVFRTVKAGSELVFEGSILSHRGRNMIQDNIFEGSYQNKNCVDCACGAKDDDGERMVSCDICEVWQHTRCVQIPNNEAIPSIFLCSRCEQDILALPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YR84</accession>
    <name>A0A175YR84_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_026947</name>
        <name evidence="3" type="ORF">DCAR_0832626</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YR84"/>
    <dbReference type="STRING" id="79200.A0A175YR84"/>
    <dbReference type="Gramene" id="KZM85631">
        <property type="protein sequence ID" value="KZM85631"/>
        <property type="gene ID" value="DCAR_026947"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM85631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH13117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="122" mass="13510" checksum="C0E695EDEF839E24" modified="2016-09-07" version="1">MTSVAQRNKISESDGLYHGRRSDDFSLDSSLHSAVQGIGKLQQELSVTYNFAAAAKSADQLTPVLTKSPSKATGMFSGTQDKCATCGKTAYPLEKVCNFFKNIRYYYTLLIKLKLLFLLLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W2B6</accession>
    <name>A0AAF0W2B6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W2B6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166:SF74">
        <property type="entry name" value="OS05G0256350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32166">
        <property type="entry name" value="OSJNBA0013A04.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="1">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="201" mass="23164" checksum="83F78B2130083157" modified="2024-05-29" version="1">MKPPSYHEVRVPLLKEEVEDAKQLMKAHEAEWVAHGCSLMCDGWEDRKHRTLINFLVNTSRGSFFLESVDASAVAKTGDMLFDVISRFVERIGPKNIVQVVTDSASNHKAAGRMLMIRYKHLFWTPCVAHCIDLMLEDIFGIERLNKCIKKAMMLNSFIYTKSGMVNMLREFTGRREFLRPSKTRFATTFFDIVQDQHSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3D5</accession>
    <name>A0AAF0W3D5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0101450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3D5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="192"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG82287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="246" mass="28619" checksum="6D8414471F35E9B3" modified="2024-05-29" version="1">MCIAEQNLDPSLHNLEHTFMLYQSMILVLELSKRIKQLEVDGRSWYLFYNVPNGYIYNLEDMLKHIKVIEKEAIVFPMNSSNVITARIFQKDGMEICYKRRIRSAKYLGDEHWFFKPDLRTDYEDDSEDSSESAGGNAGDDEDMEEMICSSPSQSRACLLIRKHMFIPIAIHPPNRAWNKGDEVELRTEKGSWRLGMVLHGNRARLSAGWNKFARENEYKVDDVLSWQLMEENDTDVFVVTKVAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3E8</accession>
    <name>A0AAF0W3E8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glutamate--tRNA ligase, chloroplastic/mitochondrial</fullName>
            <ecNumber evidence="5">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0101465</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3E8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.350:FF:000004">
        <property type="entry name" value="Glutamate--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="11 18" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="20">
        <location>
            <begin position="72"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="21">
        <location>
            <begin position="410"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG82302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="593" mass="67037" checksum="F3DD965A3FD6B80B" modified="2024-05-29" version="1">MAIVFERMTRMRFRCRITGVGPPLFRQAFPMSSMLPDCHHNKIICLIPASTKRCLSTVSTSTSSIEKDDNFVRVRFAPSPTGNLHVGGARTALFNYLFARLFIPFLIFLLILPRFSHFYTDLERSTRESEEAVLKDLSWLGLHWDEGPDIGGNYGPYRQSERNALYKQYAEKLLNSGHVYRCFCSNEELEKMKEIAKLKQLPPVYTGKWASASDEEVQEEIRKGTPFTYRFRVPKEGSLKINDLIRGEVSWNLDTLGDFVVMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFQMPYFAHVSLILAPDKSKLSKRHGATSVGQFKEMGYLPNAMVNYLALLGWGDGTENEFYTLEQLVDKFTIERVNKHGAVFDSTKFRWMNGQYLKLLAPQELAKLLGHHWKRTGILMESEGLFVEEAVELLKDKVDLISDSENALTNFLSYPLFETLSSSEAKPVLGDSLSEFANSLLAAYDSGELFDALNQGKPGWQNWVKSFGKSVKRKGKSLFMPLRVLLTGKLHGPDIGASVVLLHTLGSGDIVASEIGFVTLEERIKLLKEVEWKSFNNNQTVLEPAIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4S9</accession>
    <name>A0AAF0W4S9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOG81708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF170">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="7">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="183" mass="19349" checksum="99A7A7DC3D0354FD" modified="2024-05-29" version="1">MSSQKDFKGDSQVSGGPSQPAPRKDLHTKVEGRDSRIRMPTLCATRVFQLTKELGLATDGETIQWLLQQAEPAIIRATGTGTVPAIATVTADGTLRVPQRKEGAAEDVSKTSGLAPVGPSPVSVPGYGMAAENGMRMGGPHGAEPNQNQTSVSCEARVSSDAEEEYDEVVLMGKKIRFRKGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6S6</accession>
    <name>A0AAF0W6S6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0103445</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF14">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="8">
        <location>
            <begin position="536"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="717"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1296" mass="145740" checksum="A98040F6A55C6CF5" modified="2024-05-29" version="1">MDDNLSTNHKLCGYLCTILQTDPPIAPNLISTTCEIFAEGSETGFICENGTVLSTINGETEYAVGEGSNTASSSRKKWSGIGLVNGSISVVNQLYALVSHKCLSIVSKVVEVVEVVGKAVRVVVLVDVYLPIQLWSGWQFPRSATVAAALCRHLSCNWEARRSLLQLGNTDDQRSDNIWNISDCHVLGCKLQCVAPGTSKKKLFELHDIFMSLPSLSMKGGSDLSRVYPADDTSDSGIWDVSDDVLINIFATLGPIELLRVSATCRHLRSLAASIMPCMKLKLFPHQHAAIEWMMQREKDSEVLPHPLYLDLVTEDGFTFYVNVVSGEIVANIKPVFRDFRGGMFCDEPGLGKTITALSLILKAQGTLALPPDGAEVIWCTHNGKQGCGYYEISADKLASGQALPTTRVVGQTPRRGQFHLDDSQEKLVHSAKRTRVMGSAKIVAESANKLSPHEEAGSPQAACVAHCSSKQSRSKKNLLDAFDEESDYNTGTARRSSRKRRHPSNWPFYSSLEKDSSSDVSSYRRKRGNKITNDSDNKQTWIQCDACRKWRKLTELDAAKTTTAWFCSMNSDTFHQNCLVPEEIWDARQKLTSLPGFHTKGKSGGEEENISFFITVLREHSALIDSVTKKALTWLAKLSADKLLEMETVGLLQPVMQPQAARRGNIHQFHRVFKAFGLIKREKEGTTKWYYPRTVSNMDFDVAALRIALCEPWDSIMLYLSKATLIVVPSNLVDHWNTQIQKHVRPGQLRVYIWTDHKKPLAHNLAWDYDIVLTTFNRLSAEWNPHKRSVLMQVHWLRVIFDEGHTLGSSLNLTNKLQMSISLTATSRWLLTGTPTPNTPNSQLSNLQPMLKFLREEVYGQNQKLWENGILRPFEAGMEEGRLRLLHMLQRCMISARKADLKSIPPCIKKVTFVDFTEEHGRSYNELVVTVRRNILLADWNDPSHVESLLNPKQWKFRSTTIKNVRLSCCVAGHIKVSDAGGDIQETMDLLVENGMDPSSEEYAFIRYNILHGGNCMRCNEWCRLPVITPCRHLLCLECVALNSERCTYPGCDNFYEMQSPEILTRPENPNPKWPDDWHPDWQSTSSSKVNYLVHRLKELLGGNQNISCPINGGVDLKQVSETTSCSEPSNESNVAPSEKIIIFSQFLEHIHVIALQMKSLTTFQHDPDCIALLMDGSAALGLDLSFVTHVFLMEPIWDKSMEEQVISRAHRMGATRPIQVETLAMNGTIEEQMLNFLQDADECRKLLKEGTGKHDRESSRTRRSLHDFAESNYLTQLSFVRTVPRLEGLQLAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W742</accession>
    <name>A0AAF0W742_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0103640</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W742"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000001">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000014">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000126">
        <property type="entry name" value="Valine--tRNA ligase chloroplastic/mitochondrial 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="72"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="687"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Valyl-tRNA synthetase tRNA-binding arm" evidence="15">
        <location>
            <begin position="859"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="857"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG84457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="936" mass="106914" checksum="C245654988972B3B" modified="2024-05-29" version="1">MALPNPSLLSSSSLYRLNPLLFSQRRRSITLSSLHFTSLKPRLFTASASSESDVFTSPEVSKSFDFTNEERIYNWWESQGYFKPNFDRESDPFVVPMPPPNVTGSLHMGHAMFVTLEDIMVRYHRMKGKPTLWLPGTDHAGIATQLVVERMLASEGIKRSELGRDEFTKRVWEWKEKYGGTITNQIKRLGASCDWTKERFTLDEQLSRAVIEAFIRLHEKDLIYQGSYMVNWSPNLQTAVSDLEVEYSEEPGFLYYIKYRVAGGSRSDFLTIATTRPETLFGDTALAVHPEDERYSKYVGMNAIVPQTYGRHIPIISDKYVDKEFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNDVAGLYSGLDRFEVREKLWKDLEEIDLAVEKKPYTLRVPRSQRGGEIIEPLVSKQWFVTMEPLAEKALKAVEKGELNIMPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIVARSSDEAITKAREKYGKNVEIYQDPDVLDTWFSSSLWPFSTLGWPDVSAEDFKRFYPTSMLETGHDILFFWVARMVMMGIELTGKVPFSNIYLHGLIRDSQGRKMSKTLGNVVDPIGTMQEFGTDALRFTLALGTAGQDLNLSTERLTSNKAFTNKLWNAGKFVLQNLPKQNDTSALSTMLVYEFNKDECLLTLPLPECWVVSKLHILVDTVTTSYDKFFFGDVGREIYDFFWSDFADWYIETSKARLYNSDGGAVSSVAQAVLLYVFENILKMLHPFMPYVTEELWQTRAIRNARAEYSVEPAKRISASIVANSEVTQYISKEKEVLALLSRLDLQSIHFTDSPPNDANQSVHLVAGEGLEAYLPLADMVDITAEVQRLSKRINKMQKEYDGLVARLSSPEFIEKAPREIIDGIKEKASEAEEKLTLTKNRLTFLESTTVVAGSIHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7R6</accession>
    <name>A0AAF0W7R6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0102387</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7R6"/>
    <dbReference type="KEGG" id="dcr:108206393"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000558">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF45">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="375"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG83213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73056" checksum="D1E886A04135C913" modified="2024-05-29" version="1">MLKIFRPQNKSNSTGKLPFLSFKLSYCSSNSQPSSSFASSHTQPLYLTNAILNCKSTKRALKLFYSAPKHISYKNTLELHSAIIHVLNDAKMYLQARCLIKCLLELLLKTRSSKRACSSVFDAISELKSKKGNFNVFGMLIIALSEMSLVEEARWVFGKLGVLPSVQACNALLDGYVKMGWFDSMWELYNFMLERGLVPSVVTYGILIDACCRRGEIEKAYKLFDEISYKRITPNVVIYTTLICGLCGDGRILEAERLFKTMQEAAVLPNLHTYNALMDGYFKLANVYAALGLYQEMLRKDVMPNVVSFCIIIDGLSKEGKAAAAHSYLVCMIKFGAIPNLILYNCLIGSYSREGNLAAAVKIFSEMESYGYAPDAFTYNTLIKVYCSEGGLEKAHHLFHEMNKYGITANSVTYNSLIDGYCKKGNMDEASKLCSNMMETGIKPNVITFSTLIDGYCKIRNMEAAMGLYIEMVIKGLEPDVVTYSSLIHGQFKDGNTKAALRLYTEMEEAHVTPNAFTVSCMIDGLCKNGMIAEAIKYFLDKPISQLSGTNIAQMDHEGCLANNVTYTALIQGLCKDGQISTASKLFSDMRSLDLRPDVLSYYTIMKGHLKVKNVLDVMVLQADMVKLGIIADSLICDMLTNIHHEVGDLTSALKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBD0</accession>
    <name>A0AAF0WBD0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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            <tissue evidence="3">Leaf</tissue>
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    </reference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
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        </recommendedName>
    </protein>
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    </gene>
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        </lineage>
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    <reference evidence="24" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230"/>
        <property type="match status" value="1"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <source>
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    <sequence length="281" mass="32637" checksum="3CDF1A49FE57E029" modified="2024-05-29" version="1">MAYRFGPILLGQMHFRLQIWARPNSNIHSYSVALAFLAQEEHAASRLHKNNDGNADEVHCSRERSRAAWKIIEEYLMPFVEQEKYQLSQQCRLHPDNDIYRDQEQHKIHVDINEWRCGYCKKSFRSEKYLDQHFDNRHSNLLNISHSKCLADLCGALHCDLVTDFKAKKNKCNPSASARNKHLCEGLADKCFPINQGPSANRLHELFLRQFCDAHTCSGGHKPFSRGSKKHTSIFYLVISILTSLLVAIFYIVVYVYRRGMRKDSQGFKRISRRGQKPKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJK1</accession>
    <name>A0AAF0WJK1_DAUCS</name>
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        </recommendedName>
    </protein>
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        <name evidence="17" type="ORF">DCAR_0208776</name>
    </gene>
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        </lineage>
    </organism>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJK1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000006">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000029">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000003">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000014">
        <property type="entry name" value="Polymerase (DNA directed), eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21704">
        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="16">
        <location>
            <begin position="14"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="529"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="590"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG89538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="682" mass="75719" checksum="5BDC3B21311C0133" modified="2024-05-29" version="1">MPVARPESSDSRVIAHVDMDCFYVQVEQRKQPELRGHPTAVVQYNAWKGGGLIAVSYEARSFGVKRSMRGDEAKSVCPEIQLVRVPVARGKADLSIYRNAGSEVVAILSRKGRCERASIDEVYLDLTDAAEKMLSETPPESLEVIDDEVIKSHVLGISAEPSDVRQAVREWICRSNADRRDKLLACGAFIVAELRMQVLKETQFSCSAGIAHNKMLAKLASGMNKPAQQTVVPFVSIKSLLEPLPIRKMKQLGGKLGCSLQDDLGVNTVGDLLQFSEGRLQERYGINTGTWLWNIARGINGDEVESRVLPKSHGTGKTFPGLNCLKTIAAIERWLSKFSEELAERLEVDLEQNKRIAQTLTLHASAHKATESQSQKKFPSKSCPLRYGTTKILEDALNLFQAGLREYLSSSSKAYGNGCSGWGITNLSLSASKIVDIPSGTCSIMKYFHTQDQSCSSTKEFNDKEIFEAATLSTSGSESYLMKHLQEPQTECHEDGTKMNTLMQTHLEDESRMFEDKACAQTNFSREISSLSTPVKKNEPQLDSVGEDAVIKDAAGGNRSRDQKGKALKEKQGTSSILQFFQTKNCFQEPKEESVNDMQETTESSCSVPSESGGIRSLHRGADQSRVDCIYKIDEIDPNVVDELPREIQEEVRAWLRPQKRANNTMRRGSSITHYFSPNKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJQ3</accession>
    <name>A0AAF0WJQ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formyl transferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0209598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJQ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08648">
        <property type="entry name" value="FMT_core_Formyl-FH4-Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041729">
        <property type="entry name" value="Formyl-FH4-Hydrolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004684">
        <property type="entry name" value="PRK06027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706:SF1">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036480">
        <property type="entry name" value="FormyFH4_hydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="3">
        <location>
            <begin position="47"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG90354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="27649" checksum="5F050DEB1B94455E" modified="2024-05-29" version="1">MITCSEFVFDPAKWTRAQIDEDFLNLSTRFKAVRSIVRVPDLDPKHKIAILASKQDHCLVDMLLGWQDGRLPVDIKCVISNHERGPNTHVRRFLERHGIPYHHVDTTKEDKSEEGILDLVQDTDFLVLARYMQVLSGNFLEAYGKDVINIHHGLLPSFKGGNPSKQAFEAGVKLIGATAHFVTKELDEGPIIEQMVDTVSHRDNLPSFVQKSQNLEKQCLAKAIESYCQLRVLPYEQNRTVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNB2</accession>
    <name>A0AAF0WNB2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </evidence>
    <sequence length="2160" mass="232889" checksum="FEC7F3B9CC86C9CF" modified="2024-05-29" version="1">MIDFLQIAGLETGRVLKCSLGKLAKFPAPVLRMLESSSTQENRKSDGSTSLDDPDGIISRVAKLVEQLHANKSSPLEKELITTRLLGITKARKEARTLIGSHAQAMPLFISILRSGTPVSKVNVATILSILCKDEDMRLKVLLGGCVPPLLSLLKSESFEARKAAAEAIYVVSAVALLDDHVGTKIFTIEGVVPALWEQLKPCNKTDKVVEGFVTGALRNLCSDKDVFWRATLEAGGVNVIVGLLSSSNDVAQSNAASLLSRLLIAYSDSIPKIIDSGVIRALLRLLGKENNISARASAADALEVITLKSTEAKKAVIDEEGVPLLIRAVVAPSKECMQGEGAQVLQVRAAQALANACGGISSLILYLGELCQSPGSAAPIADIIGTLAYLLMIYEQKYDTEEDSFDPKKIENILTLLLKPLDNKLVRECVLEAMASLYENTFLSRTIEQPHSKRLLIGLITNAVDDIQDSLILSLISICCDGIDIWEAIGKRDGIQLLVSQLGLSSEQNQEYAVGMLAVLSDQVDESKWAITAAGGIPPLVQLLELGSEKAREDSAHILWNLCCHSEDICECVESAGAISSFLWVLKNGGPGGQEASAKALTKIIRVADSPTINQLLALLQEEPPNSKVYVIKVLGHVLAMASQRDLVDKEAAANKGLRSLVQVLHSSDEETQGYAASVLADLFSSRQDICNTLAMDEVVNPCMKLLNSKTHFIAAESARTLGRLIRPIKAKSRNKMFPIAEGNVKPLIKLAKNSFIAAAETAVAALANLLSDPQIAAAALAEDVVSALIRVLINGSLEGKKSASRALLQLLRHFPVCDVLKDSAQCRSTVLEIVSSLKEMDMKLNEAAESLEVVALIFRTKQSGNLGFPPWTALAEVPSNLDPLVRCLADGRSSVQDKAIEILSRLCADHPALLGNSLVANSRSIASLAKRIIKSTCLEVRVGGTALLISAAKERKSNTIDALFASGYLMPFVYALVEMTRRNSGCHSLEIEVATPRGSTEKPPFQEADDFEIPDPAIILGGTAALWLLSIISSYHVKNKKLVVEAGGLEALSEKLAHYTASPQAASEDSEGIWISALLMATLFQDASVVSSPATLHIIPSLSVLLESDEVIDKYFAAQAIASIVCNGDKVSKLAVANSGVIVRLINMIGFVELDMPNLVALSEEFSLVLNPDQVVLNCIFELEDVRAGSTARKTIPLLVDLLRPMPDRPGASQFSIHLMTRIAEGSDANKLLMAEAGALDALTKYLSLSPQDSVESMLSELLRILFSNHEILRYEAAISSGNELIAVLRFGSRSSRFSAARALNELLNAENIRNSELAVHAVQPLIDLLNVGLESEQHVALLSLIKLTSGDSSRSALLSCVEVNALETLYRILSSNSATEFKTNAAELCFVLFGSSSIRAMPIASKCMEPLILLMRSDIDTAVEAGVCALERLLDDEHNVELAAGCDLLDLLVSLVSGSNSRLIEASISVLIRLGKDKTPCKLDIVEAGVIENCLLLVPSAPSSLCSTIAELFRILTNCSTIAKSTSAAKIVKPLFILLLRPDFSLWGQHSALQALVNILEKPQSLDILKLTPSEVIEPLLSFLESPSQAIQQLGTELLSHLLAQEHFQQDITTKNAIIPLVHLAGTGILNLQETAIKALENLSLIWPKALAEAGGILQLAKVIIQDDPQPSHALWESAASVLSNLLNFDADYYSRVPLVVLVKMLLSSSDSTVSMALRALVFSEKNEPSSGELMAEAGAVDALVDLLRSHRCEDTSGRLLEALFNNVRIREMKVCKYAVAPLAQYLLDPQTRSQSGRLLVALALGDLSQHEALARGSDAVCACRALVNLLEDQITDELKMVAICALQNFVTHSRTNRRAVAEAGGLLVIQEMLLAPSPDVAAQAAVLINILFSSHTLQEYVSNELIRSLTAALEKELWSVATINIQILKTINVIFSNFPKLHVSEAATLCIPHLIAALKSGSEVAQDYALNSLCLLKDSWSSMSVDVSNSQATVAAEAIPVLQMLLKTSPPSLHERADNLLHSLPGCLTVTIERANNLRQILGGTNAFCRLMIGNGPPQQTKIIYNSTSPQWNQGFMWSFDVPPRGQKLQIVCKSRSTFVKTTLGRLMIQIDRVVSEGVYSGSFSLSHGSHKERSSRTLEISITWSNGMPADNELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYI9</accession>
    <name>A0AAF0WYI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYI9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF13">
        <property type="entry name" value="PROTEIN FORGETTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="567"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="506"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1158" mass="129323" checksum="9862B19490C0BA42" modified="2024-05-29" version="1">MEHHPAVLRNSPPPPPPPEPLPGGCQISCANCDEVHTVSRSVSEFVCFGCGLVQAIGRADEVAIEVEREEDSGGVAGETFTDYRPPKLSVGPPHPDPIVETSSLSAVQPPEPTYDLRIRGDLERTEALSCLQIETLVYASQKHLQRLPDSTRAGFFLGDGAGVGKGRTIAGLILENWQYGRKKALWISVGSDLKFDARRDLDDVGAKFIEVHALNKLPYSKLDSKAVGVKEGVIFLTYSSLIASSEQGRSRLKQLVQWCGKDYDGLIVFDECHKAKNLVPEAGQRPTRTGETVLEIQALLPEARVVYCSATGASEPRNMGYMIRLGLWGTGTSFLDFPDFIGSLEKGGVGALELVAMDLKARGMYLCRTLSYKGAEFDVVEVPLEDKMMVMYNRAAEFWVYLKEKLESEIALLTTKMPKNSQLWLLYWGNHQRFFKHMCMSAKVPAVVKICHQALAEDKCVVIGLQSTGEARTEDAVAKSGIELDDFISGPRELLLKFIEESYPLPEKPASSSGEDGVKELQRKRRSVTTGVSYTERTRKAAKLQDESDTESENDGFHESMESNNGFHICEICNSDTESDKLLQCSRCKQVMHPACLVPPVTGSVSADWSCLLCEGKAEEYLRQKRKSIAQQLERYSKAAERKSQILDAVRSLDLPLNPLDDILDQLGGPDKVAEITGRRHRLIRERDGNGVRYQPRNSKDVSMEMVNINEKQLFMDGKKLVAIISEAGSAGVSLQADKRAINQRRRVHLTIELPWSADRAIQQFGRTHRSNQASAPEYRLLFSKLGGERRFASIIAKRLESLGALTQGDRRAGPSLRAYNYDSPYGKRALAEFYDRIVNQSSLLDIPPGFSIEKPNTIEEFIIKGKAALASVGLVKDNSYDIHNVARFLNRLLGLQPEMQNLLFEFFVSILDLFVQNARLEGQFDSGIADLKANTIKLKGLPKNVHVDHLSGASTVLYTFTLDRGFTWEAASTLLDEKKKDMIGSSRIGFYESRREWQGRRHYLLAFQGSSGMFRIVRPATGEAVREMPLLELEEKYSKLKSLKKARTCWENEFRVSSKQCMHGPNCKLGDYCTTGRRLQEVNVLSGLILPVWATIEKTLANQVRKSHRRLRIVRVETTTDHQRIVGLLIPNAVVNSMLKDLVRGQDINDSLALEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZT9</accession>
    <name>A0AAF0WZT9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VDE lipocalin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0417362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZT9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046422">
        <property type="term" value="F:violaxanthin de-epoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015994">
        <property type="term" value="P:chlorophyll metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010028">
        <property type="term" value="P:xanthophyll cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19420">
        <property type="entry name" value="lipocalin_VDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044682">
        <property type="entry name" value="VDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010788">
        <property type="entry name" value="VDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33970:SF1">
        <property type="entry name" value="VIOLAXANTHIN DE-EPOXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33970">
        <property type="entry name" value="VIOLAXANTHIN DE-EPOXIDASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07137">
        <property type="entry name" value="VDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VDE lipocalin" evidence="2">
        <location>
            <begin position="100"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="437" mass="49270" checksum="B8E454CA6F777BBE" modified="2024-05-29" version="1">MVKLWSNCKRSTPTQVIRNLRVYCGLDSRFPDILSNGRAKIFSLCNIEAVMKSKDPFNLETTVKLIAQKQWSQFAAVALVLACTFVIIPNADAVDALKTCTCLLKECRVELAKCIANPSCAANVACLQTCNNRPDETECQIKCGDLFENSVVDQFNECAVSRKKCVPRKSDVGEFPVPAADVLVKSFNINDFTGKWFITSGLNPTFDAFDCQLHEFYTEGNKLVGNLSWRIGTPDGGFFNRSAVQKFVQDPSQPGILYNHDNEYLHYEDDWYILSSKLEGTPEDYLFVYYRGKNDAWDGYGGAFVYTRSAVLPESIVPELERAAKSVGRDFNTFIRTDNTCGPEPPLIDRLEKTAEAGERTIIKEVEEIEQEVEKVKDTEVSLFRRLAEGFKVFLEDEQNALNGLTKEEEEILSGLQMEAAEVEKLFENALPIRKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X540</accession>
    <name>A0AAF0X540_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ent-kaurene synthase</fullName>
            <ecNumber evidence="11">4.2.3.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0519266</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the biosynthesis of ent-kaurene diterpenoids natural products such as oridonin, miltiradiene, eriocalyxin B and nezukol, known to exhibit antitumor, anti-inflammatory and antibacterial activities, and in the production of gibberellins phytohormones. Catalyzes the conversion of ent-copalyl diphosphate (ent-CPP) to ent-kaurene.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ent-copalyl diphosphate = ent-kaur-16-ene + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22220"/>
            <dbReference type="ChEBI" id="CHEBI:15415"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58553"/>
            <dbReference type="EC" id="4.2.3.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22221"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the terpene synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.19" evidence="11"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X540"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009899">
        <property type="term" value="F:ent-kaurene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033332">
        <property type="term" value="P:ent-kaurene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.160:FF:000002">
        <property type="entry name" value="cis-abienol synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000002">
        <property type="entry name" value="Ent-copalyl diphosphate synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000005">
        <property type="entry name" value="Ent-kaur-16-ene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739">
        <property type="entry name" value="ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31739:SF3">
        <property type="entry name" value="ENT-KAUR-16-ENE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01014">
        <property type="entry name" value="Terpene_Cyclase_Like_1_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="12">
        <location>
            <begin position="225"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="13">
        <location>
            <begin position="498"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG99910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="791" mass="91100" checksum="5DF08D114C8B4709" modified="2024-05-29" version="1">MNRTTPHRNFVVWFTRFLYYAVRLCMIPTNQFNNLFHFTMSSMLVIEESKERIRRLFRKVELSVSAYDTAWVAMVPSPHYSHAPCFSGCVDWILENQLKDGSWGLPQQSVRLLKDDLSSTLACVLALKRWDVGEEHINKGLRFLKSNFASVTDNNQSSPIGFDIIFPGMLEYANDLGLKLPVEQTLLNTVLNKKAQEIKRCSERNSPESEAYLAYLSEGMGNLQNWDMVIKYQRKNGSLFNSPSTTAAYLNHIQNTGCLNYLSHLLEKFGNAVPTVYPIDIYAHLCMVDTLERLGIDRHFKQEIRSVLDEAYSCWLQDDEEIFMDVDTCALAFRILRMNGYNVLSDKLTRIAKEECYLNSLGENLKDTNEALQLYRASEAIIYSNESALEKQNSWSNRFLEHKLSNGSVHLDRCARIIFQEVHDALKFPFHSNLERMVNRRNIEQYEADSIKILKTSYSSPNISNAEYVRLAVEDFNVCQSIQQKELKLLESWVIESSLDKLKFARQKTAYCYFSAAATLFSPELSDARMAWAKNGILTTVVDDFFDVGGSIEELLNLIQLVEKWDVNEETECCSEHVRIIFSALHHTICEIGESAFKRQAWHVTTHIIEIWLELLNSMLKEAEWTRDSYVPKLDEYMSNGFISFALGPIVLPTVYLIGPELSENVVQNGELRSLFKLMSTCGRLLNDIQSFKRESKEGKLNAVSLYMIHTDATTEEDAINEIKCAIEINRRELLQLVLQEKDSVVPRACKDLFWKMCRVVHQFYIKDDGFTSEDMFGAVKDILYKPITRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5T5</accession>
    <name>A0AAF0X5T5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0519675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5T5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF8">
        <property type="entry name" value="ZINC-FINGER DOMAIN OF MONOAMINE-OXIDASE A REPRESSOR R1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="388"/>
            <end position="456"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
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        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
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        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
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        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH00316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="695" mass="77041" checksum="6BFFD9E93CCDC806" modified="2024-05-29" version="1">MAVNSIPQAASNQNKSQSETKQRTTDPGVRVIGGKIYDSEKGKTCHQCRQKTRSFTAACKNKKKDKTCTMLFCHKCLLNRYGEKAEEVEVLEEWICPKCRGICNCSCCRKKQGHQPTGILTHRAKATGYSSVSELLQANGPENFGLVKNVKDTGATPKRPSSSGKQVTSPGKKGKENLLDGRVDMNLEHPTPSKLKEIEKMQGVTLENNEKQKKTKQGKLRTVSAGQKDAAALKLCSSGALPESTPDKSQDSDTVSAGPKDAAAQKLCSFGTLPESNSNKSQDSDISIHMHAEPNEKVNDPKLGDGCMKKVSAEVPADTFGNKKRNANKDIEVIDQEKTKKRVKHDTGMPFVKDNNSGLQFQSQEVGNDIPLPHGTELITIGDLELPPEDVGNALQFLEFCAAFGKNLNMKKGQSSVILKELMNGQSRILRRTEQHSPVVHFHIQLLSLLRKDYTLRSHKLSPSHGKSSWLFALKSCLSESPNAKDLLPIIGLDSESGGYNTLNFSMKLRILIFLCDEVLETERIRDWINNQESKFAQKAKQAKKKVLAAKDKVKSLKQKLADELAKEITAKDGSSLSISEHDEIVSQIKCEAAQAHKEMLESMNMVPKAKKRSQALRTQPLFLDGDGRAFWRLNCYPEESNILVQDIGNGDDSIVFGEKWITFDNEQEKVVEKHISLRERMVRAQSAAIIPYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X6B9_DAUCS</name>
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            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">DCAR_0522010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="11"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="196" mass="22514" checksum="56D10BCFED2D3B93" modified="2024-05-29" version="1">MNVSLEGEEKLILVPYMKEHVPKYHQWMKDPTLLAATGSEPLTLDQEYEMHQSWTHDPSKHTFIVLDKDLILPQFVHGQAHVEAMVGDVNIYMNDLEDSRIAEIEIMIAEPKSRGKGLGKKSVLMMMVFAVEKFCIHTFRVKIGESNEASLRLFRKLGFVDASYSKIFQEVTLELVITKLKMEELHLLAGKFIEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X927_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0622858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="15242" checksum="0A985DE7238908CB" modified="2024-05-29" version="1">MDSYHSQFFGGYEEECQSSESGWTMYIGSPADDDDDGEAKNDVEEQYKGGGGDVESDDSMLSDASSAMTSNIKKKDVYHQEAKKGDVELKKNARKSMKKGAEKIEKPEKKKDEKSTSMNAAPQSGNKVRRNWLGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAZ3</accession>
    <name>A0AAF0XAZ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0623147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAZ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF85">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4 HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="83141" checksum="B3C60C8FC416D2CD" modified="2024-05-29" version="1">MRVRFSAASSLLATAMSSRPPYRGSRRFSDAGINQFVSGDAHFQSVRDANQRRGRTDHHSHPQLNSRVSHSDYRPPQSNSNAAPLRYHPNQAFRPPAFYNNQQQQLRNPQDQHFQHNQQSRNPQYFQQNQQPRPRNQKPLDYRDWEYAPNRPSPDCDRFTILSYNILADYLAIDHRGKLYFHVPRYMMDWEWRKKNILFELGLWAADILCFQEVDRFHELEEELKVRGYCGTWKMRTGTPVDGCAIFWRSSRFQLLHEESIEFNKLGLRDNVAQICVLERISQNDTRHKSAIPASPLYNFISEQALNLSDLPRDKVSGQASAEIRPSRPFPHNLRHPDIPTDGSPRASSDNRIVRQGEISPNMQKNNDLDGNNGAIPNQRDLPFSHHINSGSSGLINVGGHNVEKISDNDEAGIFHNKEDLDFHTELGSLDGSQDMHPGYLTKRAMHQPPIVHSQYEDKHSNMLSTEQGDLNKELDVSCNELHQLNLCDINYSTSNESSKHVQSHSVTNGNNHLPTLHEIKSPNCYTSDGCILDEMEDDLSDKVTESMQNRTPVEDSQASISGLHGDLFSLSESSQVSGSNYSECAGSTDDGSISEFGPHVKDSGMKFNQVDTERAAYDPSAWTPMEIEIATGCVDSRLMESPLKLRSTYSEVEDSSGTRDSNGEPMVTSYNRCFQGTVDYIWRSEGLQTVRVLAPIPKHAMQWTNGFPTKKWGSDHIALVSELAFTKDIGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XBL4</accession>
    <name>A0AAF0XBL4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0623498</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XBL4"/>
    <dbReference type="KEGG" id="dcr:108227803"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15866">
        <property type="entry name" value="R-SNARE_SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000028">
        <property type="entry name" value="25.3 kDa vesicle transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000003">
        <property type="entry name" value="25.3 kDa vesicle transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="14">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="15">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH04092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25301" checksum="6834E22B46A58E0A" modified="2024-05-29" version="1">MVKLTMIARVTDGLPLVEGLDDGRDVQNADIYKQQVKALFKNLSRGHNEASRMSVETGPFMFHYIIEGRVCYLTMCDRAYPKKLAFQYLEDLANEFERVNGSQIETAARPYAFIKFDTFIQKTKKLYQDTRTQRNISKLNDELYEVHQIMTRNVQEVLGVGEKLDQVSQMSSRLTSESRIYADKAKDLNRQALIRKWAPVAVVLGIVILLFWVRKKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFY2</accession>
    <name>A0AAF0XFY2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0626489</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFY2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF52">
        <property type="entry name" value="ULTRAVIOLET-B RECEPTOR UVR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="38"/>
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        </location>
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            <begin position="124"/>
            <end position="381"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="351"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="447" mass="47899" checksum="E2625579005A25D2" modified="2024-05-29" version="1">MEGDDEINSTRTGQGNAGHRVVLISAGASHSVALLAGNVVCSWGRGEDGQLGHGDAEDRISPTQVTALDGQDIVSVSCGADHSTAYSESSKQVYSWGWGDFGRLGHGNSSDVFIPQPIKALHGMRIKQIACGDSHCLAVSMEGEVQSWGRNQNGQLGLGTIEDSLVPQKVQAFQGIAVKMVAAGAEHTVAITEDGELYGWGWGRYGNLGLGDRNDRLVPEKVTTVDGDKMVLVACGWRHTISVTSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLHALSGEVISQISGGWRHTMAMSSTGKLYGWGWNKFGQVGSGDNLDHCSPMPVNFPHEQKVVQISCGWRHTLAVTDKQNVYSWGRGTNGQLGHRESIDRNTPKIIEALSVDGSNGQLIESSNIDPSIGKISVLPSDRYAVVPDENKAHGQTVPAAKDITTDASVPEGDVKRIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Daucus carota subsp. sativus</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="74" mass="8133" checksum="1BDC055166B63A01" modified="2024-05-29" version="1">MSAGLEVVAVSAFFGFLVSFGAGFAGLVSCCRHPFVTVSMRLRPKRTCSGVECFGGFVIMKYFYLFLFLRGPFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHX5</accession>
    <name>A0AAF0XHX5_DAUCS</name>
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            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Apioideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Serine/threonine-protein kinase involved in the spatial and temporal control system organizing cortical activities in mitotic and postmitotic cells. Required for the normal functioning of the plasma membrane in developing pollen. Involved in the regulation of cell expansion and embryo development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000372">
        <property type="entry name" value="MAP3K epsilon protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000278">
        <property type="entry name" value="MAP3K epsilon protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000304">
        <property type="entry name" value="MAP3K epsilon protein kinase 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052441">
        <property type="entry name" value="Armadillo-Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="403"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="784"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="927"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="784"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="850"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00054419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH07467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1298" mass="144977" checksum="C0931DA846A6F7A3" modified="2024-05-29" version="1">MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLGNIPQEDLNIIMQEIDLLKNLNHKNIVKYLGSSKTKSHLHIILEYVENGSLANIIKPTKFGPFPESLVAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEANINTHSVVGTPYWMAPEVIEMSGVCVASDIWSVGCTVIELLTCSPPYYDLQPMPALFRIVQDEQPPIPDSLSPAITDFLRQCFKKDHRQRPDAKSLLSHPWILNSRRVLHSSLRHSGKLRNEEECLVDVITSNGDDQGSVENLPVNYAEDSKDLLLVEAADINQCSKDKDSEHNLSEEISGDPEEDIFPDQSPTLAIYEKLEINASSFRLSSNDEADASSVFCEPSHLTAHEKVLVNDEAESLNFSRESISGGKVEVGDSSIKYGPSIPAPTDQDQSPQKALKAPMTSGENELSRFSDTPGDASLEDLFHPLDKTLEERVTEASTPKPSPILNHGSTVATDGRSDLAKQLRDAIIQKQTEIESGKRNDEDLIHLMIGVLKEDSIEGFDDRLPTDNLFHIQAVEYSKLVSSLRPDEPEDVIVSACQKLTAFFQQRPEQKFVFVTQHGLLPLVELLEVPKNRVTCAVLQVLNQIIKDNTEFQENACLVGLIPSVMSFAVPDRPREIRMEAAYFLQQLCQSSSLTLHMFIACRGIPVLVGFLEADYAKHREMVHLAIDGMWQVFKLQQSTPRNDFCRIAAKNGILLRLINTLYSLNEATRLASISGNSAFTPSEAMVSEINLPDYGKVKDGTADNSMITVKPQPSTASLRSLETPPPNPKERETIDRWKSDPTRAEFDLRQQRDSNGIRLSTDRAMKPVDGSQNGLPATSQQEHVRPLLSLLDKEPPSRRFSGQLEYVRHFTGLEKHESILPLLQTSNERKTNGLDFLMAEFAEASGRGREHAKLDSMPKSTQRVSNKKVGLPPSNEGAASTTGMASQRESDLALEYIEKVADLLLEFSLSDTTVKSYMCSQSMLSRIFQLFNKIKPAILLKLLKCINHLSTDPHCLENLQRADAIKHLIPNLNLREGPLVYRIHHEVLNALFNLCKINKRRQEQAAENGIIPHLMHFVMTGSPLKQYALPLLCDMAHASRNSRELLRAHGGLDVYLSLLEDDIWSVTALDSIAICLTHDNDNKKVEQAILRKDAVQKLVNFFKSCPEQHFLHILEPFLKIISKSREINITLTVNGLTPLLISRLDHQDAIARLNLLRLIKAVYEHHPHPKQLIVENDLPQKLRNLIEERRDGQSSGGQVLVKQMATSLLKTLHINAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIJ0</accession>
    <name>A0AAF0XIJ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0727100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIJ0"/>
    <dbReference type="KEGG" id="dcr:108194254"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000001">
        <property type="entry name" value="Lipid phosphate phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="100"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH07667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="314" mass="34978" checksum="076A712EA4B6E098" modified="2024-05-29" version="1">MPGNQLGTHTIRTHGTHVAKLHMHDWLILMLLVVIEVGLNVIEPFHRFVGEGMMEDLKYPLQDNTIPIWAVPIIAIALPLAVIIVYYFIRKDVFDLHQAALGLLFAVLVTGVLTDSIKDAVGRPRPDFFWRCFPNGKGVFDPITKNVMCTGLKSVIKEGHKSFPSGHTSWSFAGLGFLSWYLSGKVRVFDRRGHVAKLCIVFLPLLVASLVGVSRVDDYWHHWQDVFAGGLLGLTVASFCYLQFFPPPYDMDGWGPHVYFQTLADLASENGGQTSSSNINCLGIRPAELENVYIQSHNGVVSPMLNGVESGRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJ69</accession>
    <name>A0AAF0XJ69_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">DCAR_0728530</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJ69"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000565">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase protein FLORAL ORGAN NUMBER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF596">
        <property type="entry name" value="OS02G0215500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042168051" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="27"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="645"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="704"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="733"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="WOH09075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1006" mass="110571" checksum="60842B1F0DB874DF" modified="2024-05-29" version="1">MMPYVISEVVCVILISLLLSNCKFQALDGNATDRQSLLAFKKSIVQDPQNALGSWNDSLHFCQWEGVTCGRRHQRVTRIDITSRALSGSLSPYLANLTFLQILQINNNSLYGEIPPQLGNLFRLKALNLFLNNLEGTIPANISRCSNLQHLRVGRNNLVGKLPEELGALSKLQSLVIEENSFTGRIPPFLGNFTSLEIFYARRNHFSGGIPDTIGNMRKLSILGLAANNLSGMIPLSLYNISSLTQLSLDENELQGTLHPSIGSMLPLLQVIQLSDNQFTGLLPLSLSNCSKLEIFQMNTNFFEKKIAINFGGQNSLMNIVLDENNFGTGETDEMNFIGSLSNCSNLQGLGVSDNKLKAVLPDSVGNLSTKINYLNLAGNQLYGRLPSSIGNLINLDTFGLDDNQFSGTIPISIGNLEKLQVVAFSQNKFSGVIPQSIGNLTLLTKLYMNMNRLEGNIPINLANCSRLLTLELSQNNFTGLIPKQLFSLSTLSITLNLSHNYIYGQLPSDVGNLVHLGSLDLSNNRLSGKIPASLGSCSSLEYLFLQNNMFQGSIPSSLSSLKGVMNIDLSLNNLSGEIPKFFEQLSVKYLNLSFNNLEGEVPTRGIFSNLSGVSVLGNSNICGGMSELHLQKCANGGSRKQERYIRVLIVLFSVIGILICATILMWFMFRHKRLGNLESGGPMVDSSHLRLSYADLHKATAGFSLENVVGSGSFGCVYKGVLDANRMTVAVKVLNLHSRESYKSFMNECEALRNTRHRNLVKIITTCSSVDFQGNEFRALVYEFMPNGSLEQWLYPSLQSQRQPRLTLAQKLNIAIDVSHAVDYLHNQCEKPILHCDLKPSNILLDNDMVAHVGDFGLAKILHPGIAEVTQSSSVGLRGTIGYAAPEYGLGSEVSKSGDVYSFGILLLEMITGKRPTDAMFNEGLDLHKFVSTALPDHLQHIISPTFHEDFEETIETTKISGENVVQECLLKVGTACSVESPQARMSITDVVSELNFIRQTITFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XLN6</accession>
    <name>A0AAF0XLN6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein Lines C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0729521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XLN6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029415">
        <property type="entry name" value="Lines_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032794">
        <property type="entry name" value="LINES_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024875">
        <property type="entry name" value="Protein_Lines"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16057:SF1">
        <property type="entry name" value="PROTEIN LINES HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16057">
        <property type="entry name" value="WINS1, 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14695">
        <property type="entry name" value="LINES_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14694">
        <property type="entry name" value="LINES_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Lines N-terminal" evidence="1">
        <location>
            <begin position="449"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Protein Lines C-terminal" evidence="2">
        <location>
            <begin position="632"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="78210" checksum="CA95DB1F51A8CB23" modified="2024-05-29" version="1">MRRQPENTRRLCRLINDSLAPFNSITELSIPIPKQTEKQLLISLSNTARQIKLWTEEYDDSDSDTDAKETDHTVCTDEDHHCLTKIVIELVSLLEVKNIYVQHIAGNILVVISKFLAASGSCYDKYVYLLALCLELSTCNCLQTLEPSTRFDESHSNLSASFLVLKQSLKSASWYTAAGVISVLRNMLKHLKMECDDQLLKGYLNSVRSCILNIPWNVFEEVSLGEMYLQHEDALNRNKLQNLPSLTLFNGNLVQFFCSLAAHGSASETSAAYMNEQPVACIIGNVMPKILGWCLRKQDHNNTRTSQYLRHKILKLMVRLTYQMHLQCEVLVSWLNIIGKYFQDLLAEPLTRVENNMGDSLEGSPFLISFSKDNKGISDHHLQRLAVFLFLRCSLSLVCQRDGTDERCVCDEEKLNHSCCNRRQGILGLYQWLRGHIAQDMFADNDIYIQKCTSFSLSFLQLFMHEDDILFKVLLQLFTVPLSVKPVSRGSITLQKVEDEDNMYRHISDLLNPICLFHLFLAELLYDYQVLLDYLISKDTGASSAEYLLRCLRAVCDSWISFVEFSWDEEVTNYTKFKKRKVLVDVLDLEGGESSVQRETDDSSLSLTKRCMRKDVYTSRQHQTTRPPFERAMECLLSLKSSLLNLHRKKLFPYNPDVLLRRLTKIEELCLKQKNLLTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XLT4</accession>
    <name>A0AAF0XLT4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0728849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XLT4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF113">
        <property type="entry name" value="CARBOXYLESTERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="82"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH09392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="35616" checksum="57BAC33B20AA69E9" modified="2024-05-29" version="1">METESSAINEAYKHLKAVPNPDGSLTRSEIFPSVPPTLQLQANSPSETQLALSKDIHLHNSNITFLRLFRPANHAAKLPVIIDFHGSGFVVCSAASAPFHEMWTYGSAFVPALIISVEYRLAPEHRLPAAYDDAMEAVMWVRDQAREVNGRDPWMEELADYSNVYIMGASAGGNIAYHANLSALDADISPVQIKGIILNQPFFGGVQRTQSEERLSTNPSLPLNVSDLMWQLALPLGSDRDHEYSNPLIKCNPKIKQLTRCLIRGFEGDPLVDRIKGLVKMLEASGVHVVAKIQDGGFHGADVFDESKMKELFSQIKDFIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNC7</accession>
    <name>A0AAF0XNC7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0729677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNC7"/>
    <dbReference type="KEGG" id="dcr:108196723"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="544"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="623"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH10212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="75302" checksum="9824AAEFCEE76A7F" modified="2024-05-29" version="1">MNLLRKEIKGLGKKYNLLIAFSNTNTAYRLTCIDSLSSSLFHESCSKLHTNSQMGQPQNPLESNPSRTFEPICLEKKSGSFENGGSYDEFAVDVEKIYRILRKFHSRAPKLELALKESGVVVRSGLTERVLNRCGDAGNLGYSFFVWASKQPGYRHSYEVYKSMIKILGKMRQFGAVWALIEEMRRENPQLITPEVFIVLMRRFASARMVKKAVEVLDEMPKYGCEPDEYVFGCLLDALSKNGSVKEAASLFEDMRVRFTPTIKHFTSLLYGWCKEGKLTEAKFVLVQMKKSGFEPDIVVYNNLLHGYAVAGKMADAFDLLQEMRRKGCNPNATSFTILIQALCAQEKMEEAMRVFMDMQKSECDADVVTYTTLISGFCKWGKIEKGYELLDSMIQLGYTPNQTTYLNILLAHEKKEELEECIELVKEMQKIGCNPDLNIYNTVIRLSLKLGEVKEGVRVWHEMEANGLSPGLDSYVIMIHGLLEQECLVEACEYFKEMVEKGLLSAPQYGVLKDLLNSLLRADKLELGKDVWNCIMMKGCELNVFAWTIWIQALFAKGHVKEACSYCLEMMDAGLLPQPDTFAKLMKGLKKLYNRQIAAEITEKVRKMAEERQTTFKMYKRRGERDLIEKAKEKKEGRKRRSRRHQWGTNRSRGSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPH7</accession>
    <name>A0AAF0XPH7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0831065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPH7"/>
    <dbReference type="KEGG" id="dcr:108198983"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1498">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH11575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="480" mass="53463" checksum="23CD0A4E0158F951" modified="2024-05-29" version="1">MSAAASSKPHALCIPYPAQGHITPMLKLAKLLHHKGFHISFVNTDFNHRRLVKSNGLQALDGLPDFRFYSIPDGLPPSNPDATQDVPLMCKYIPINCLAPFSKLISELNNTSVSHVPPVTCIVSDGVMSFTLKAAQQFGIPEVLFWTASACGLIAYMQYSQLVDRGYIPLQDTSHVTNGYLDTAIDWVPGMKDMKLKDFPRSIRTTDINDIVLNFFITETAALPKARALVINTFDTLEEDALKAISASQPHIYTVGPLHLLMKQIQDKRLMSISSSLWIEEDSCIDWLDKKEANSVVYVNFGSITTMTAEQLSEFAWGLANSGKHFLWIVRPDIIAGDTAKVPQEFVAETKERGRLASWCSQERVLKHPATGGFLTHSGWNSTIESIACGVPVICWPFFAEQQTNCKYSCVDWEIGMEIDNNVKRNEVEQLVRELMDCERGRELKKKALEWKKKAEAAASPGGSSDLSLDRLVDELLLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQ20</accession>
    <name>A0AAF0XQ20_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0830533</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQ20"/>
    <dbReference type="KEGG" id="dcr:108197510"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH11054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="13876" checksum="2FA198039BA30ACE" modified="2024-05-29" version="1">MSAEAVNPKAYPLADSQLTITILDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADTEPLEILLHLPLLAEDKNVPYVFVPSKQALGRACGVTRPVISCSVTSNEGSQLKSQIQQLKDAIEKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSS1</accession>
    <name>A0AAF0XSS1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0832809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSS1"/>
    <dbReference type="KEGG" id="dcr:108199786"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47451">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47451:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="179"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="223"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="782"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="852" mass="93121" checksum="36E96373718F2E17" modified="2024-05-29" version="1">MALSLAARFTFNLPNQSLQTHAQVFTFRTPSRILTTRSSSFFLATHLKPLVKINSTGFKVLTRACSDGGGDKIDASTSSDIKASTISPSTSGDEYVALFVRMLGLDNDPLDRDQAVVALWKYSLGGKQFIDAIMRFRGSINLVVNLLKSESTSTCEAAAGLLRTISAVNAYRDLVGESGAIEEIFGLLRRSSLVSDVKEQSLFTLCNLSVDEKFRSKIVHSDLLPILIKLLEDEDIKVVEAAGGVLANLALSQSSHKIMVEAGVIPKLAKLLTTDAEGSKVIRKEAKNVLLELAKDDFYKILVVEEGLVIVPLIGTAAYKSFRPALHSWPSLPDGTEIKQSSKGPSRFGASELLLGLNVQDKNSDLEEAKMNAIVGRTQQHFLARIGAIELEDQQIFNGESSSDRRITILPWMDGVARLTLILTLEDESAVARAAEAIADASISEHMRVSFKEAGAVKHLIQLVGHHNVIVRLAAISALDRLSLSNNVCQRIEAEGVLYPLINSLKNSEIPGKGADMILNILNRILDPSREMKFKFYDGPVNGSKRVDAKENIESAQNVDEIGVSRSTKSSQNIHVEYTSDSAFLVCLVEILKTSAPVSQRKAASIFEFMAVDQSCMEKITSVDIASGLDAVFKQSILKGIESNFDHQQPELHALEVEEAGHAISAASRLLTRLLDSDRFCQSINSLHFTKQLREILRSSIPLHNKDWVAACLVKLSSLSGPFPGFEDPINREVTLYETVPRLIQQIKNSFSPEIQEASVIELNRIISEGVVNSSRAVAIEGGIFPLVKLIEEGTDRAAEAGLAILYNLSMDSENHSAILAAGAVPVLRRIVLSQRPQWTRALHLLRTLPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTM0</accession>
    <name>A0AAF0XTM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0832636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </evidence>
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    </evidence>
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</entry>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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        </reaction>
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        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="PROLINE IMINOPEPTIDASE"/>
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    </dbReference>
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        <property type="entry name" value="PROLINE IMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG89623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="dcr:108209768"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-ribosylation factor GTPase-activating protein AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000011">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044518">
        <property type="entry name" value="ARF_GAP_AGD11/12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="3"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="146"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35303" checksum="50C617C7FB31046C" modified="2024-05-29" version="1">MSKRKLKDLLLQKDNRVCADCSAPDPKWASANIGVFICLKCCGVHRSLGTHISKVLSVTLDEWSDDELDSIVEVGGNASANSIYEAYIPAGVSKPRPSASQEERVNFIRSKYESQDFLKPSLRILSTSSKASLRTSFSSKITDSFRSSSSTSHKLEGMVEFIGIIKVKVIKGTNLAVRDILSSDPYVILKLGKQKVQTTVVKSNLHPVWNQELMLSVPQNYGALKVEVYDYDTFSADDIMGQAEVDIQPMINSAMAYGDASMFDDMQIGKWLKSNDNALIHDSTVNIIDGKVKQEVSLKLQNVESGELDLELEWMPLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ART7</accession>
    <name>A0AAF1ART7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="9">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0312155</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RdRP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="9"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ART7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058697">
        <property type="entry name" value="RDRP3-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058752">
        <property type="entry name" value="RDRP_C_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26249">
        <property type="entry name" value="4HB_RdRP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26253">
        <property type="entry name" value="RdRP_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="6 9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP3-5 N-terminal" evidence="12">
        <location>
            <begin position="8"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP core" evidence="11">
        <location>
            <begin position="235"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP C-terminal head" evidence="13">
        <location>
            <begin position="921"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF26249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF26253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG92878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1003" mass="113935" checksum="EB5D55E40CA5689A" modified="2024-05-29" version="1">MGDPQTPIPLPERVETMIEQICKEQKQPHPDADARKILASIGEEKSLHMLTIISQTQIKKTFSGFIKYLLKNYRPKPLSHAMSPKSTLSTPHKRTPSSVSGEHSLASPCSTCGNSYANPRKKLRFSPHVSINRESTSAISKQWQILFELEFRKIFLILNYIGRLKLEEVVSEEDAYKILLMKSYPMERFESQLWTMYNGFSFAGTVSTDRRKYLDWECGETHHYHCHVWQDGSFSFKGPFLKTTKTLLHRTFGDENILIVKFEEQARGGSSIISGDHNAFYGRVAKEGILVGQRRYRFFVFKDGERKGKKNSPTTSDVKCYFIRTELIAHCDTKDPFNLFNKTMHEARCLFMHLHTTPTMAKYASRCSLILSQTINVEVNLDRVHIERIEDIPCRNENGIKVCNEDGDLLIQTDGTGFISEDLAMKCPRALLSARYRKAKESEMEDASLEGKLTDAHVREPPLLMQCRLFHNGLAVKGTLLVNRKLPLGTIQIRPSMVKVEADPNFEGAPSINSLEVVNISRKPGKSRLSKYLIALLYLGGVPEQFFMNILADALEDSQRLFSDKRSAVRASVNHGTIDDDFTTARMILSGVPLDEPCVQHRLSHLAYSERKGLRQGRLPISESFFLMGTADPTGLLNSDEVCVILDNGQVSGKVLVYRFPGLHFGDIHVLNAVYLKELEDVVGNAKYGIFFSTKGERSVASEIANGDFDGDMYWVSRNNQLLHYFKKSTPWTRMHPSPNKFQVKPSDIPNDELEHKLFKLFLDTKNQNISMGVAADSWMAFMDKFLTLDDNSVEKNKLKKTILKLIDIYYDALDAPKTGKEVTIPKEYRADSYPHYMEKGKAYDSTSVLGKICDAVDTYVPKRVLASEIKKLPLFELVPIPDAKLKFWKEIYDNYRTDMSSALSSTNQSEMKPADDVFSKYKKILYGAADFEQSPRKWDDIYVDAVALYHACYDYARSKGDVTKCNFAWRIAGHALCNFHVAKSAEKSSMFYSLPSVLGEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATT2</accession>
    <name>A0AAF1ATT2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0311421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the 3-hydroxybenzoate 6-hydroxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATT2"/>
    <dbReference type="KEGG" id="dcr:108210397"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044560">
        <property type="entry name" value="MOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934">
        <property type="entry name" value="FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934:SF2">
        <property type="entry name" value="MONOOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="14"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG92161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="401" mass="44687" checksum="2BAC1298B717D4DD" modified="2024-05-29" version="1">MNKLGGTEGEVQHEIVIVGAGICGLATALALHRKGMQCIVLERSESLRYTGGAIGIMANGWRALHQLGVDSILRQTANPIVGTKDIWLDKNKQREMSFMSGEARCLRRSDLINTLYNALPPNVVKFGHQIVSVKLDPQTNFPILQLEDGNSISSKVLVGCDGANSVVADFLQLKHTKVAVLCSARGLTNYPSGHPFTHEFVRMKRNNTFVGRIPIDSKLVYWFVAHPWVQTDTKISQDKELIRQYTLQLVKSFPKETVELIKDTDHESLSFTRIRYRRPWDLLLGSFRKGTVTVAGDAMHVMGPFIGQGGSAGLEDAIVLARNLAKKMSMTPTDPRSIEEALDEYVKERRMRIVRMSAQTYLTGKLITESTPLLVKFACIILMILLFRDASGHTKYDCGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AV20</accession>
    <name>A0AAF1AV20_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0415329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AV20"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000035">
        <property type="entry name" value="RNA polymerase II C-terminal domain phosphatase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF0">
        <property type="entry name" value="RNA POLYMERASE II C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="8">
        <location>
            <begin position="131"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG95999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="664" mass="76174" checksum="97A49156B02A9E64" modified="2024-05-29" version="1">MSQLGLKCKVYYENNCIGELNIVAVEGSNFEFPNNEIRIHCLSPKSNRCSPHSVAHMIASSPVLCKLEAEDKYSDLFSLYIDCIRDRQTGVVLLGEEELHLVAMVNKYKNCPCFWCYTVPSGLYSVCLRMLDLRCLWIVFDLDETLIVGNTMRSFRDKIASLRIKIAKETDPVRKSGMFAEMALYEEDQRLLKQYIDRDSVVDSGNVYNVQAEVVKICESQDRIVRPVIRLLDRGIVLTRINPEIRDTSVLARLRPAWEDLRRHLNSKERKRFEVYVCTMAERNYALEMWRLLDPEAQLIDPKQLSARIVSVNSGHKKSLNRVFQDQKFHRGMAMVIDDRLKVWEDIDQPRVQVVPAFLPYRAPLAETANGIPVLCVARNVACNVRGCFFKEFDDNLSRRVCRLFYEDEVKNLPLPPDVSTYMIPEFEPSISEVLELGPEMNIASHVAQEAASDIEIPYLMCSPCYFGFMMEGISAWYMLQLHAYAASCKYWKSLYQLKSTSIQLSDGNLPITILYSYILLFVCAGLFWWSERCIKGNPLPMLWVRPSGGGALRPRCGGVRRCGSIRTECATWRIIIYVVVDTWSDLNIHVECDASDIGIGIGGILSEEGHLIALFMEFAHMIKDYPFCRDFGKIYASLWYSSSPSPREFCIHDGYFLIPIYMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWS2</accession>
    <name>A0AAF1AWS2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0417619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AWS2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF120">
        <property type="entry name" value="LYSOSOMAL PRO-X CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042116082" evidence="6">
        <location>
            <begin position="25"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG98278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="474" mass="53677" checksum="71725F61E3B04984" modified="2024-05-29" version="1">MRVLQFSCSLIFLLLCSALCLSYAAPRFPGSVIKPEQISSSTVAKSENELYKTKYFTQILDHFNYSPQSYHKFQQRYLINDTYWGGAKKNAPIFVYTGNEGDIEWFTQNTGFMFDIAPQFKALLVFIEHRFYGKSIPYGGNKEVAYANSSTLGHLSSTQALADYATIIIDLKKNLSATDSPVVVFGGSYGGMLAAWFRLKYPHVALGALASSAPILNFEDITSPYSFANIITQDFRSESENCYKVIKGSWQKIEDTAKQPGGLDLLQKSFHICNVVVCLNVQMCKAIDNPVHGNDTFAKLYAAANIYYNYTGHAKCFDLDDHSDPHGLDEWSWQACTEMVMPTDGSNEESIFPVSEPSYKDWAESCHDYYGVWPRPNWITTEFGGHDIKRVLKRFGSNIIFFNGLRDPWSGGGVLEDISKTIIAIVAKEGAHHVDLRYATKEDPEWLKEIRSREIKIITKWLSQYYTSLFHLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1P2</accession>
    <name>A0AAF1B1P2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0520008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1P2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
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        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="163"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH00637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="383" mass="43968" checksum="6614E6F61612B59E" modified="2024-05-29" version="1">MEQKLTTLLQHKWLSKLLGYDYMVVYKRGKDNLVTDALSRKYEDVPQCNAIHTVVPLWKQELTASWATDKWAQELSASLMLVDGELMKDGCWYVGTGNDMREKIIKNVHSSLEGGHSGVTATTKRIQERFYWPNLQTDVLKFVRECDTCQRCKPEHVAAPGMLQPLPIPNNAWETMSLDFIEGLPKSAGKEVILIMVDKLTKYGHFIALNHPYTATVVAQKVLDTVVKLHGPPKVIISDRGPVFVSSFWRELFKAMGTQVSLSTAYHPQTDGQTERVNQCLEMYLRCVTGLKPQSWCSWLPMAELWNNTCHHSAIGMSPFKALYNQDPPFVNYQVGDVTNVRYKQFLQDRTKVQHLLKENLLKAQERMQWYANKKRTNRQFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2T0</accession>
    <name>A0AAF1B2T0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0521253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2T0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH01867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="161" mass="18441" checksum="310F5E41ECC54967" modified="2024-05-29" version="1">MAIRVSFFAPEIAFSLAELPSQYQPELPLCLSSDLLFVNTSQHGVGQISNRTFARRLAPSEGNFNHWKLMFFHSDTYFQAYISGRNDLLWQMKIILDNCHSKDSFSGLEMHVSLIKLKRSLSGELNIFPRLLILPLTTYTELRHRRGDLGFSCCLRPSSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B317</accession>
    <name>A0AAF1B317_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0623719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141052">
        <property type="term" value="F:histone H3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013257">
        <property type="entry name" value="SRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08236">
        <property type="entry name" value="SRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="10">
        <location>
            <begin position="480"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="379"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1416"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH04310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1748" mass="191378" checksum="A33F26DCBDEAD6EE" modified="2024-05-29" version="1">MECKNGLCDGSVEESGLGFNDSMHIGVEKMGDEGGKVGGKKRVMETRVGVDSNDEEPIGAMMAKKKNKCNPKKVNEGFVGEGIEEKESGVEDLGGMGDTLAIYKKKLKRSRKSSVSVGIGDKDLDIIGHEGCGQESSAEKVKEEGVVVEEGKTVVVEGTCGTVGGLNECLDFNVEDSLSAFFLKAQSVSVRKSRTSMKSKQLGKIYSLSDGLSAGSGRVLEDQMPETSISQSPLEHMTEIVGFNSSSNLESENTMDLKHSCNETTKLISGKHLGSFECLSEKKLTSHETNLLMSKHDEAKEEKALSPSSLGVEKQEIDFQENPSWNCKQNGDKSDGLTEHALINKSDTSVPRCTSSFHLTHSSDNAFKEPSALSCDFSGDEVADGSSSPSTAPDCNKNHTDDTGLVPSPKDKDNKLSAQRAMRNTRKRRQLDMAYEGDADWNFLVHDGNFNADNQDDDNSLVRRKEKSKPSTMFLEAKNGCNAAVSYGLKARAASPIEKIRFKEVLKRKGGLNMYLECRNHILGLWNKDVSSILPLSSCGISATPMVDESPDASLIREIYEFLDHFGYINIGIASKKRVSESSLVYNLHASTKKTLGETPGAALADSDEGVSFIVGKSRNPDSSLEEKNLALHDCENVVAMDKQDRPVLDSHVLESFILTKPEKSDSNQGQGLLDGMESVTLGSLDNLNFVSPCKVADAGTAPVIPSEPMRKINRTLYDTVDNVGENPCGEHHSEVAKQIIIIGAGPAGLAAARHLQRQGFQVTVLEARGRIGGRVFTDRTSLSVPVDLGASIITGVEADVAAERRPDPSSIICSQMGLELTVLKSDCPLYDTVTGHKVPADLDEDLESEFNNLLDDMELLVAQKGTHAMKMSLEDGLEFALERRRLTKYKRNGGRSILHKLADVIDEEVLRNSNSEGEVLSPLERRVMDWHFAHLEYGCAASLKDVSLPCWNQDDVYGGFGGAHCMIKGGYSTVVEALGEGLCIHLNHVVTSVTYRTGDAMIDEDQHHNVKVSTSNGRDFFGDAVLITVPLGCLKKESIKFAPPLPHWKNLSIQRLGFGVLNKVVLEFPEVFWDDSVDYFGATAEETDKRGHCFMFWNVRKTVGAPILIALVVGRAALDVQDKSSSEHVNHALAVLRKLFGVAVVPDPVASVVTDWGRDPYSYGAYSYVAIGASGEDYDILGSPVENCVFFAGEATCKEHPDTVGGAMMSGIREAVRIMNMLTTGIDYMTEVNSMEAEQRCMDSERSEVNDIIKRLEAVQMSNALYEKSLDGSHVITRKDLLQDMFSKAKTTSGRLHLIKELLHFPVRVLKSFAGTKDGLSILNKWILDSLGKDATQLLRHCVRLLTLVSTDLLAVRVSGIGKTVKEKVCLHTSRDIRAVASQLVNVWIEVFRKQKASNGGLKLMRQSNAVESSKSKYSLGSGKPPAPHGAQPSHNKKGINPTKMLDEIQYVPSEEEKAILAAEEAARAAAIAAAKAYALSGTRGSTSLQLPKIPSFNKYARREHYPQLEEADNHTLEIDSRNCKVREWTADFSSSHVNLGNSKRSVENLPQRSSLHEVACQVNFSEHSADSAAVDSSIFTKAWVDSAGSEGIKDGSAIERWQSQAAAADSEFYSRTMRMMDAEETKINSRPSIMKHEVHASESSTFQVTTGKEMVENRSKGPEKIKQAVVDYVASLLMPLYKAKKFDKDGYKSIMKKATTKVMDQATDAEKSMNVFDFLNSKRKHKIRAFVDMLIERHMASKSGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3L2</accession>
    <name>A0AAF1B3L2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein DA1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3L2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045218">
        <property type="entry name" value="DA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022087">
        <property type="entry name" value="DA1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209:SF31">
        <property type="entry name" value="PROTEIN DA1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209">
        <property type="entry name" value="PROTEIN DA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12315">
        <property type="entry name" value="DA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein DA1-like" evidence="1">
        <location>
            <begin position="192"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="400" mass="46516" checksum="823D7CC387EBFA5B" modified="2024-05-29" version="1">MSFSENDFAILSLNYEKYENEDVHYAANYTIPEANSPEMNSEVEFYGVHLSELLTGDLEPSVSCNMKTSMTKGKRWWGKLFSGLNIIKKFNLESSTSKWDYNCQADKVCDVCEEAIHSSWVTRPYWGQIVCYKHISDGTPMCCSCLRFKLGNTRYINLSDGRKLCSECHSTAIMDTETCIPLFQEVYRFFEGLNMEIIKDIPIFLVDKHEINKIRYEFGGKSVRISLFLLFIYYSFKEHGISSGNDNIYLFVARSFQRGDNIHVVKEVQYIQRIYEVTSLLLVYGFPRLAIGATLAHEMMHSWMRIEGYKGLREEVEEGICEVMAHRWLDWHAFIGDDFLHTTPEQAQFLRNLKEVLKNDIERNYCKTYGGGFRDAKWAIERFGLKYTMSHVAQTGNFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4J2</accession>
    <name>A0AAF1B4J2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0623157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4J2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000503">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF388">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="7">
        <location>
            <begin position="604"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH03757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="696" mass="77718" checksum="489AC7C39A266057" modified="2024-05-29" version="1">MAIIYLRSLSKACNYTYNSTVSIPLLKHLSTVPARFDPNYQNPSNCDGVNEQQSSGFYAESEYDAREKFKGNFVNQAVHQTAGGQYFQDRRNENLNGKIGSYGQENGGSEFRNDSGRFSREGRVNEFRGSGYYGQRSGEFSQQPSGFNGSQSSLNGVPGAGVVQQRQNQFYGVGSSEGNASYGQHAMHSQWSSANHSVGNVNNVGNTQQGTNNFNGNNCYGNVVGSHQQSQNSYPGNYAENFNGNLMRNVGGYQPTPSVEYRTNAGPLNIPNEYQNDIGGFQVAGSSTSGGGMVDASEKHTVEEIDGLCGEGKLKEAIELLIQLEQLGNAIDKDQYFRLMKVCGEAQALEEAKHVHDLFNKRNFPYEVRNFNNILEMYCICGSMDDAYDVFTKMPQRNLTSWDTMILWFAKNGRGEEAIDLFTRFKELGLQPDGEIFFSVFTACSFLGDITEGLLHFKSMSKQYGIVPLMQHYVAVVDMLGKTGYLDEALEFVENMPVEPSVDVWETLMVSCRINGDMELGDRCAELVELLDPSRLTEQAKAGLIPVKASDIAKEKEKKKLISRNILDVRSRVHEYRAGDTSHPDNDKIYALLRGLRQQMKEAGYVAETKFVLHDIDHEGKEDALLAHSERLAVAQGLISSPARQQIRIIKNLRVCGDCHNAMKIISKLVGRELIMRDAKRFHHFKDGVCSCRDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5Q5</accession>
    <name>A0AAF1B5Q5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0624384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group II-b family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH04972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="WRKY domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
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        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 9-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="220"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="464"/>
        </location>
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    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="57"/>
            <end position="91"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="55"/>
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        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04972.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein phosphatase inhibitor activity"/>
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        <property type="term" value="F:signaling receptor activity"/>
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        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
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    </dbReference>
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        <property type="entry name" value="Plant_def-hormone_signal"/>
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        <property type="entry name" value="START-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="Bet_v_1"/>
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    </dbReference>
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        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
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            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07312.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="16503" checksum="FD18BCCE5273A8A9" modified="2024-05-29" version="1">MVLQKHEQEITSSLSADKIFNGLIVDVDTIFPKAAPGAYKNVEIKGDGGVGTIKHITLPDGSPVTTMTLKTDALDKEACTVEYSIIDGDVLLGLIDKVETHLVVVPNADGGSTTKTTTIFHTKGDAVVPEENIKYSEEQNISVFKAIEAYLIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B9H7</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0729141</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B9H7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038943">
        <property type="entry name" value="PLDrp1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33971:SF1">
        <property type="entry name" value="OS02G0743600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33971">
        <property type="entry name" value="OS06G0232000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="28180" checksum="DADB89AB192D3916" modified="2024-05-29" version="1">MSYYPKGHHGEDEVDDFDEYDPTPYGGGYDIHLTYGRPLPPSEETCYRANSSGSGGGFDYNRPQYSTQAKPSAYGSDAVDDEYKSYNRPTTRPGSRPGASGGGVDESEYGGGGSGGYGRKSGHDSEYGSGGGGGYGSGGGGYGSGGGGHHGSGGGGYGHHEQVGSGYGRKNDDDEYGSGYGRKSGHEQHGSEYGSGYGGRSERTEYGGHESRVSEGYGGSGYGRSEESDDDEKKKRYGHKKYVSFIHYQPFYILNKSCYNSSYALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BA55</accession>
    <name>A0AAF1BA55_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0728367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BA55"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34364:SF9">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34364">
        <property type="entry name" value="WAS/WASL-INTERACTING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH08916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9111" checksum="FF47830E187F0527" modified="2024-05-29" version="1">MVQSVGVRYKLVWRLLIISNLALGAYMFATPRRKSTGKEDATLKAESKARSLTAISSPPIVTMEPVDVDLPIPENQKQELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XY91</accession>
    <name>A0A161XY91_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_010431</name>
        <name evidence="8" type="ORF">DCAR_0311834</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XY91"/>
    <dbReference type="Gramene" id="KZN01677">
        <property type="protein sequence ID" value="KZN01677"/>
        <property type="gene ID" value="DCAR_010431"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212428"/>
    <dbReference type="OMA" id="HMAFFWS"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF85">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="3 6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN01677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="15086" checksum="65AC4CF135CED43F" modified="2016-07-06" version="1">MDMPMSPPASTDMATQMNFYWGKDVIILFQGWPSNNLAMYILALAFVFMLSAATEVLSIPQVVRPSSGSRPIISLLIQASVYAVKMGLAYLVMLSVMSYNLGIFIVAVAGHGLGFLLVKIRALAAAGPANQAGSEFVDPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y1L3</accession>
    <name>A0A161Y1L3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_011813</name>
        <name evidence="4" type="ORF">DCAR_0313343</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y1L3"/>
    <dbReference type="STRING" id="79200.A0A161Y1L3"/>
    <dbReference type="Gramene" id="KZN03057">
        <property type="protein sequence ID" value="KZN03057"/>
        <property type="gene ID" value="DCAR_011813"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215421"/>
    <dbReference type="OMA" id="IICTQAT"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000112">
        <property type="entry name" value="CTD small phosphatase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="87"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN03057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG94052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="302" mass="34249" checksum="C9FF26AD1EF9E8C8" modified="2016-07-06" version="1">MAELGSGEVYAPPRTIQAWKTILNWLSFFFQIFLQIIIKASPSKFSQLVSTTAFRPLPDVELSELTQEDAPPTAAVTVEEPGGRRDWPSEKLTVVLDLDETLVCAYETSSLPAILREQATEAGLKWFELECISSDKEVEGKPKVNYVTVFERPGLREFLKQLSEFADLVLFTAGLEGYARPLVDRIDDINCLSRRLYRPSTISTEYREHVKDLSCISKDFCRIVIVDNNPFSFLLQPVNGIPCIPFSAGQPHDEQLLQVLLPLLKHLSQQQDVRPVLYERFHMPEWFQKHGIPACAPGLRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZK62</accession>
    <name>A0A161ZK62_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_023494</name>
        <name evidence="2" type="ORF">DCAR_0726954</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZK62"/>
    <dbReference type="Gramene" id="KZM86360">
        <property type="protein sequence ID" value="KZM86360"/>
        <property type="gene ID" value="DCAR_023494"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM86360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="52" mass="5541" checksum="5D561A2E5F0110A4" modified="2016-07-06" version="1">MSPCLSNHTQEPLIASSATLGSSNTKMVVTLLRSRSSGDALGILHCRKLEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZVE4</accession>
    <name>A0A161ZVE4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_020633</name>
        <name evidence="17" type="ORF">DCAR_0625724</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZVE4"/>
    <dbReference type="Gramene" id="KZM92002">
        <property type="protein sequence ID" value="KZM92002"/>
        <property type="gene ID" value="DCAR_020633"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225017"/>
    <dbReference type="OMA" id="NEYKDET"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000006">
        <property type="entry name" value="Membrane steroid-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="15">
        <location>
            <begin position="66"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZM92002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH06299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="180" mass="20217" checksum="C30DD4DFD89B3350" modified="2016-07-06" version="1">MGLYATVTDTISYYTGLSPAAFFTILALMYVVYKVVCGMFVAADDYRAVKRSNDFVLREPVQLGDVTVEELRAYDGSDPNKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALALMSFESKDLTGNIDGLSPDELEVLQDWEYKFMEKYMKVGQIVSEKSKAEHTEDEDKVHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A2C4</accession>
    <name>A0A162A2C4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_017305</name>
        <name evidence="4" type="ORF">DCAR_0519786</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A2C4"/>
    <dbReference type="STRING" id="79200.A0A162A2C4"/>
    <dbReference type="Gramene" id="KZM94060">
        <property type="protein sequence ID" value="KZM94060"/>
        <property type="gene ID" value="DCAR_017305"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219900"/>
    <dbReference type="OMA" id="GHERWWK"/>
    <dbReference type="OrthoDB" id="1923043at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35490">
        <property type="entry name" value="BACTERIOPHAGE N4 ADSORPTION B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35490:SF2">
        <property type="entry name" value="BACTERIOPHAGE N4 ADSORPTION B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM94060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH00426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="453" mass="50271" checksum="1D33093954953258" modified="2016-07-06" version="1">MPTFTTVALDTLIEPGGSKTTVAGKYVPDQKLDSRDATSSKIERMGSLPSSKSNSAIDVPKPRMERRNSASATDINSKRQFNRISPALYATPETTPLPVVPDSPSSFPPSPYIINHKRRGPRLLKSLSQEDVATREQDVEKDKVAVDAKNEEREEIDTDKDAEFTFNVPPPIDVAHDKGFPNGGLENSELRNSLNTQSGPLKSVAVNERRDGEPDDFFDPQDSLSVKSNTSNGGEQPFTRATPSEFYDAWEELSPDSVPQQLPLNEVETELREIKSSLLMEIEKRKQAEESLNNMQSHWLRIRQHLSTVGLTLPADPIGAVEDSNLDDPVEALCQQVNLARYVSSSIGKGIAKAEAEAEMESQIEAKNFEISRLWDRLNYYEAVNREMSQRNQEVVEKARKLRKSRKKKLRWVWGSLAVAITVGTAAVAWSYIPSGKGTSSSNQSDAPRSSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A438</accession>
    <name>A0A162A438_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pectate lyase superfamily protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_018242</name>
        <name evidence="7" type="ORF">DCAR_0520878</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A438"/>
    <dbReference type="Gramene" id="KZM95000">
        <property type="protein sequence ID" value="KZM95000"/>
        <property type="gene ID" value="DCAR_018242"/>
    </dbReference>
    <dbReference type="OMA" id="NWFRSET"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF9">
        <property type="entry name" value="PLASMIN AND FIBRONECTIN-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831850" description="Pectate lyase superfamily protein domain-containing protein" evidence="5">
        <location>
            <begin position="30"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM95000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH01494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="51254" checksum="90A181B1562B0457" modified="2016-07-06" version="1">MFMFLNCQQLVALILELLLFGKPLWVVSSSSLCKETSSGIDRPHSVSITEFGAVGDGLTMNTKAFENAIFYLNSFADKGGAQLFIPKGQWLTGSFMLISHLTLHIDKDAQILGSTNSNDWPVIDPLPSYGRGRELPGGRHRSLIYGCNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMVELIDSKDVIISNLTFRNSPFWNIHPVYCSQVIVQNVTIIAPLDSPNTDGIDPDSSDDVCIEDCYISTGDDLIAIKSGWDEYGISYAHPSTNIIIHRLVGQTTSSSGIAIGSEMSGGVSQIHVEDVHFFNSNRGFSIKTSRGRGGYVRNVFITGVILSNLKVALKFNGNYGEHPDESYDPDALPSIEKISIQNVTGKNIKVAGHLEGIEGDTFKHICLSDITLDVRSKSPWICSNVEGFSEMVSPDICKPLEPKINPDSKCYQMSNHLRSIRPRNKGTSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A6E7</accession>
    <name>A0A162A6E7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_019332</name>
        <name evidence="8" type="ORF">DCAR_0522123</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A6E7"/>
    <dbReference type="STRING" id="79200.A0A162A6E7"/>
    <dbReference type="Gramene" id="KZM96090">
        <property type="protein sequence ID" value="KZM96090"/>
        <property type="gene ID" value="DCAR_019332"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220953"/>
    <dbReference type="OMA" id="TCKMEPT"/>
    <dbReference type="OrthoDB" id="752464at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04873">
        <property type="entry name" value="ACT_UUR-ACR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="152"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM96090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH02734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="324" mass="36831" checksum="511A57304B6723AE" modified="2016-07-06" version="1">MEYSGHDLLEELLSLRTDPWDFATNFPLEMNDFYNNIAACSNFDHFLENPFLDTTQPFCFEEFSSFPFDQQSLNVSSTFNVSFGNEIATSVPQLTYDSSSFNTPSYVPLEEEYSSRAVANSCKVELTQSPELPVFNQGVCSEKKTKGKRLNGEQSKNLMAERRRRKRLNDRLSMLRSVVPKITKMDRTSILGDTIDYMNELLQRIKNLQEEMNPNQHELTSASILKNVKPNEVLVRNSPKFDVERRNVDTRIEICCAAKPGLLLSTVTTLEALGLDIQHCVISCFNDFGMQASCSEEMDQRGDISPEDMKQALFRNAGYGGRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AR45</accession>
    <name>A0A162AR45_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM89913">
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        <property type="gene ID" value="DCAR_022724"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN04643">
        <property type="protein sequence ID" value="KZN04643"/>
        <property type="gene ID" value="DCAR_005480"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207319"/>
    <dbReference type="KEGG" id="dcr:108225852"/>
    <dbReference type="OrthoDB" id="1741277at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
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    <dbReference type="PANTHER" id="PTHR47723">
        <property type="entry name" value="OS05G0353850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723:SF19">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="165" mass="18695" checksum="950B03D4231D1D48" modified="2016-07-06" version="1">MTPWNKPPAGILKINVSGHSDEQIRLSSIGCLMRSTSGHFFSGYYGTRDFADPLYTDLLAIYYGFKLADDEEQRYIEVESDSATAVHLVNNPNQNANYSDILLNIRRLKDMAAPSCILRYVERSSNLMAIRLSSYSIQKRVPITHLNSCPAELFEELAADWYYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AW34</accession>
    <name>A0A162AW34_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0208475</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A162AW34"/>
    <dbReference type="Gramene" id="KZN06673">
        <property type="protein sequence ID" value="KZN06673"/>
        <property type="gene ID" value="DCAR_007510"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206934"/>
    <dbReference type="OMA" id="WLINGIV"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF22">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="165"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="294" mass="33233" checksum="F2E3C8EEAC20BA66" modified="2016-07-06" version="1">MALLSAHWTLVFGLLGNVVSFMVFLAPIPTFVKVYKKKSTEGFQSVPYVVGLFSCMLWIYYALLKSNTLLLITINSVGCVFQTTYICLFLFYAPKKARIQTLKLLVAMNVVGFGLIVMLTQFLTHGALNRVSIVGWICLVFSLCVFVAPLGVVRQVIRTKSVEYMPFLLSFFLTLSAVMWFFYGLLLKDYNIAVPNVLGFTFGIVQMVLYMMYKNTKKVLAKDAKLPELFKDAEVIIIDDKKLPELKETIMNVMRLSEIVCSEMKPAAKNMDDEKELDMIQVQVVPNKSMTALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SQ76</accession>
    <name>A0A164SQ76_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_023600</name>
        <name evidence="13" type="ORF">DCAR_0727070</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SQ76"/>
    <dbReference type="Gramene" id="KZM86466">
        <property type="protein sequence ID" value="KZM86466"/>
        <property type="gene ID" value="DCAR_023600"/>
    </dbReference>
    <dbReference type="OMA" id="LCHGGSA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000577">
        <property type="entry name" value="Homeobox-leucine zipper protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF8">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM86466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH07637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="21607" checksum="3C9D77E7ED9A966D" modified="2016-07-06" version="1">MRDLDINQVPSEEELIEEDDQNPNHDGAPPRKKLRLTKQQSRLLEESFRQNHTLNPKQKETLAMELKLKPRQVEVWFQNRRARSKLKQTEMECEYLKRWFGSLSEQNRRLQKEVEELRAMKVAPPTVMTSHSSDPLLASTLTMCPQCERVTTTTAQPNAGPIRPSASKPSAMAAAKVPVIHSRHSSPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TXA4</accession>
    <name>A0A164TXA4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-amylase</fullName>
            <ecNumber evidence="4">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0728933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TXA4"/>
    <dbReference type="Gramene" id="KZM88115">
        <property type="protein sequence ID" value="KZM88115"/>
        <property type="gene ID" value="DCAR_025190"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196219"/>
    <dbReference type="OMA" id="AVMDHQH"/>
    <dbReference type="OrthoDB" id="1660156at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008540">
        <property type="entry name" value="BES1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001371">
        <property type="entry name" value="Glyco_hydro_14B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF47">
        <property type="entry name" value="BETA-AMYLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05687">
        <property type="entry name" value="BES1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00842">
        <property type="entry name" value="GLHYDLASE14B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH09476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="695" mass="78107" checksum="961DF5716CC57B5C" modified="2016-07-06" version="1">MSSEMQKFQTSEDDDDEMGMDVKEEDDEDEDDDDKNTANQVMVGVEGGFTSTSSNNRFQQNQQYQEQLTPQGGSRRCRPMEEKERTKLRERQRRAITAKILTGLRRHGNYNLRVRADINDVIAALAREAGWVVLPDGTTFPSRSQGTKPAAVTPTTVVTSSSTQMTPQQTSPASLGGMSSSYQSTVEYNACQMKDIFVPSSSPYDVTSNAPSQTSALLVEGLDVQDDPIIGGSMDAVDNRQVVIIPQRLQERDFAGTPYVPVYVMLPLGVINMKCELVDPDSLLNQLRFLKAINVDGVVINAWWGIVEAHGPQVYNWSGYKRLFQVVRDLKLKLQVVLCFHECGGNLGDDVIIPLPNWVAEIGLTNPDIYFTDRAGRRNQECLSWGIDKERVLRGRTAVEVYFDYMRSFRTEFDLFFEDGTISLIEIGLGPCGELRYPSNPVKHGWRYPGVGEFQCYDQYLLKSLRRAAETRGHSFWARGPDNSGSYNSQPKETGFFCDGGDYNGHYGRFFLGWYSQVLIDHGDRLMALARMAFEGICIAAKLSGIYWWYKSSSHAAELTAGFYNPCNRDGYAPIMAMLRKHEAGLNFTCVGTCMLVPCGDFSGELGDPEGLNWQVLNAAWDALVPVSSENDLPCHDRDGYNRILEKAKPLNDPDGRHFTSFTYLSVSLLLMERQNFMEFERFVKKMHGEAVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U1J3</accession>
    <name>A0A164U1J3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_025383</name>
        <name evidence="10" type="ORF">DCAR_0729138</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U1J3"/>
    <dbReference type="Gramene" id="KZM88308">
        <property type="protein sequence ID" value="KZM88308"/>
        <property type="gene ID" value="DCAR_025383"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193827"/>
    <dbReference type="OMA" id="SACINDH"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF586">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM88308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH09680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="369" mass="40917" checksum="AB9C4E6A1EF2A4BD" modified="2016-07-06" version="1">MGRSPCCDKLGLKKGPWTPEEDQKLLAYIEEHGHGSWRALPSKAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLANRTDNEIKNYWNTHLKKRLTKMGIDPVTHKPINDSILSSNKGTSKNTANLSHITQWESARLEAEARLVRQSRLRSSSELKSQEFPLSPLIPVSKLAMPPMPSLPTLDKLKTLNGIWSKPVSAAGSSCGSEVESPKSIVNYSGTGMGESSTSFVELMRNSSGSCEDGIMKDGTEEEWGGLRSLINPMDTWSSESLRLGYDNVHVSSENFGDKFTDLLTNNYNSGDWSYFEGNGESDNGGDETNKNYWTSVLDMVVTSPSHNMVNSCASDSPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VDK3</accession>
    <name>A0A164VDK3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0623713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHI protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VDK3"/>
    <dbReference type="Gramene" id="KZM90187">
        <property type="protein sequence ID" value="KZM90187"/>
        <property type="gene ID" value="DCAR_022448"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226607"/>
    <dbReference type="OMA" id="YFQQQEH"/>
    <dbReference type="OrthoDB" id="692274at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007818">
        <property type="entry name" value="SHI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006511">
        <property type="entry name" value="SHI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006510">
        <property type="entry name" value="Znf_LRP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01624">
        <property type="entry name" value="LRP1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01623">
        <property type="entry name" value="put_zinc_LRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604">
        <property type="entry name" value="PROTEIN LATERAL ROOT PRIMORDIUM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31604:SF2">
        <property type="entry name" value="PROTEIN SHI RELATED SEQUENCE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05142">
        <property type="entry name" value="DUF702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH04304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="335" mass="37154" checksum="1ABA5557FEBE8AE7" modified="2016-07-06" version="1">MSGFFTLGGKDQEQDQASQENSLFLFKNEEIYNKGFELWQQYYQLHQQQKTQLQDVDLSLVGGSASNYSSTRRTNDNDHTFLYRSSSSSGFGLHMRQSGVLGNNNNNSNNMIGGGGSSVNCQDCGNQAKKDCVHMRCRTCCKSRGFHCQTHVKSTWVPAAKRREKQQQLQLSSSLQQPQQDHEPQEQNQFSLMRGAHQSKRTRENPLPCTRLQNHNSGLEVGQFPAEITSSAVFRCVNVRAMDDAQEQYAYQAAVNIGGHVFKGLLYDQGPESRYVGAGENSTTSGAAQQSLNLVTTAATTTNPPGVTWLDSSTTYPTPLNAFMAGTQFFPPPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YNJ9</accession>
    <name>A0A164YNJ9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YNJ9"/>
    <dbReference type="Gramene" id="KZM94775">
        <property type="protein sequence ID" value="KZM94775"/>
        <property type="gene ID" value="DCAR_018017"/>
    </dbReference>
    <dbReference type="OMA" id="CLIVCDE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33320">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33320:SF30">
        <property type="entry name" value="OS04G0606200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH01247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="70" mass="7819" checksum="F7F958812E687795" modified="2016-07-06" version="1">MCLIVCDEQGSEVERKTASGNCPHCGGKVEAVDVETRSKLCCLIPVCFQIKRKFQCAVCFKRLVLSYYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y106</accession>
    <name>A0A165Y106_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0417095</name>
    </gene>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZYB0"/>
    <dbReference type="STRING" id="79200.A0A165ZYB0"/>
    <dbReference type="Gramene" id="KZN00547">
        <property type="protein sequence ID" value="KZN00547"/>
        <property type="gene ID" value="DCAR_009301"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210492"/>
    <dbReference type="OMA" id="CKDAHAD"/>
    <dbReference type="OrthoDB" id="1295726at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056715">
        <property type="entry name" value="DUF7813"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36353:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25105">
        <property type="entry name" value="DUF7813"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN00547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="51930" checksum="CF7EF8384ED2B1A6" modified="2016-07-06" version="1">MNHHRRRRADSPAAVNLDSATQILKQTTTIFKTHLHTLVFLSFLILTFRSNVENGSNFLTTFIDRDPSLKSLLSRIDISGRSHSSPNSNHQPILQHRRRRPFLQLTRVGTLDDDFFSGDDHSDKSLFGGIAKSNLNGTFVIFDHFDPNLGFSNGVVDSGILVNEIVRSGFTFKAPREEESLGDDAVVEDEKIGDGNERENSFGNLFKGLELGRRDATALMFVAGILSASYGYVILGFLVTYSWVLGTVFVLVLNDLLGRYKSLTSTLWDGSNLGLKRLTGFILLRWAVRDALTQLLGICFFGEIDDHYLFFKIFVRLKLMPFSVMASWVRGYEKESAGFLVSWFLFDTLLAFVFAVDAWVAIVDSRKSGREVVKEGCHLLATMLNPAINIKCLEGILCGSLTRWILSSCFGRLFASAFQSVMEVYFMVAWLVYYFAVRSKTASSLGRTFGQRELEGILEGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ALI7</accession>
    <name>A0A166ALI7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0311536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRDE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ALI7"/>
    <dbReference type="Gramene" id="KZN01452">
        <property type="protein sequence ID" value="KZN01452"/>
        <property type="gene ID" value="DCAR_010206"/>
    </dbReference>
    <dbReference type="OMA" id="MRDKELH"/>
    <dbReference type="OrthoDB" id="297219at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902369">
        <property type="term" value="P:negative regulation of RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013633">
        <property type="entry name" value="NRDE-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471:SF0">
        <property type="entry name" value="NUCLEAR EXOSOME REGULATOR NRDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08424">
        <property type="entry name" value="NRDE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG92273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1149" mass="131486" checksum="0B2B1F262A5191D9" modified="2016-07-06" version="1">MEDKPSSSSLFPVFTAPPPPTTTTTTTKQPPQWLSNTSFTTDISLINDAVSSHYATTTSLPIEHDDDDLESDEDNKHNQRPQYELVDSPASDYSSSEENKNLKKKKRKRKKQQSGVADADYKYGPSGRKSDKAWANSSSDKDYFFDSRGDRDNLAFGCIYRMDVARYKPHSSTFGNASHAKYHRNPKRRDFDADDDIDVLDSKLKSVGRYWSARYNAVERHKNFKRMRITAPEKCAMTFDNFIPLSDDTFCERSDGRTISKASVIEESWEDVVLRKTREFNKLTREYPHNEKGWLDFADFQDKVASMQPQKGARLQTLEKKVSILEKAVEVNPDNEDLLVCLLNAYRRRDSTDVLISRWEKLLVQHSGSWKLWKEFLQVVQGEFSRFKIPEMRRIYANAIRALSATRGKQHRQAYGGTIALSHDSAVIQQELNLVDLFVNLCRFEWQAGYQELATALFQAEIEYCLFCPSLLLSEQSKLRLFEYFWDSNGSRVGEDGALGWSTWLEKTEEQKQKVVNEESDEIDEGGWTGWSEPLLKTKEVDVNQEKIDENIAEVEESDELGEGEVEPEKDAATLLKMLGIDADADASDEVKNAATWIRWSQEEILRDTDQWMPLHPKTAGDTHGDGITDGEIDEHFSRVILFEDISDYMFSLTSEEARLSLLYQFIDFYGGKISQWTSTNSSAWGEKILSLEVLPDIILDKLRRVHEVLTKAKSTPMSLSLECLLDGSGDTSMRTNMMKFLRNATLLCLSAFPKNHLLQEAVLVADELSNTRMGTLSSSVTPCRALAKTLLKKNRQDVLLCGVYARREAVFGNIDHARKVFDMALSSSEMVQPDCLSNTFLIYLWYAEVELANNSGSNSESGVRAMHILYSFGSGVKYIPFTCQPSSLQQLRARQGFRERIRTIRAMGAHVVTDDRFTALLCSAALFEELTAGWTAASEVYDQAFSMVLPKRRTHSYQFEMLFNYYVRMLRKHHQQSQLGTVWGKVIQGLHLYSLSPELYSVFVEIGHLHTTPSKMRWILDDSCQKKPSVIVWLYALSFEISRGSSYHRVHGLFERALASDNTRNSVILWRCYISYEMNVACDPAAARRVYFRAIHACPWSKKLWLDGFLKLNSILTAKELSDLQEVMRDKELNLRTDIYEILLQDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AXB3</accession>
    <name>A0A166AXB3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0312284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AXB3"/>
    <dbReference type="Gramene" id="KZN02058">
        <property type="protein sequence ID" value="KZN02058"/>
        <property type="gene ID" value="DCAR_010812"/>
    </dbReference>
    <dbReference type="OMA" id="ENFMTRA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="246"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG93005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="503" mass="57195" checksum="1BEE34D0A9A0CE83" modified="2016-07-06" version="1">MFNRLNSLQLSRTDWKIKIRVTRAWDSFSSSHEFIGMNLIIIDEEDFHIHGFVIPEALEALDHPFYEGGMYIIENFMTRAAIGTLRPVTSDVCIILNESSIVTNIPLEIVRFPRYKFEITEIGDIYSVARNLDPGQPPVYALDIVGIILDKGETVVEATTPGMRQSIRFNLYDGRNMIRVVLNDEKVTLLGHIFDGDYPVDPIVILTSMRPHFRNGVLQVSSTEATKVYANIRYHVVWQIRQRIMFNSLRSLELSKTDWKIKIRVTRTWNSFSTNRELIGMNMILIDIEDYHIHAFVVAEACASLGSYFFEGNMYIIENFATRRSIGYLRPVTSAMCIILNQSTSVTPVPLELGLIARHKFEITELGDVYSIIRNLAPDQFSLKYIKAATDRFDIPVDVLGVMLDIGDVKVEDSAATPTTCVRFNLYDGRNMTRVVCSGEIVQSLLPILEGDFQTNPIVILSSMKPHFHKGVLQLSSISASKAYINISYDAVSQMRRRLIDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BB61</accession>
    <name>A0A166BB61_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_011345</name>
        <name evidence="5" type="ORF">DCAR_0312836</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BB61"/>
    <dbReference type="STRING" id="79200.A0A166BB61"/>
    <dbReference type="Gramene" id="KZN02591">
        <property type="protein sequence ID" value="KZN02591"/>
        <property type="gene ID" value="DCAR_011345"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211924"/>
    <dbReference type="OMA" id="VEQRQMS"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Gastric triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="3">
        <location>
            <begin position="295"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN02591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="696" mass="78852" checksum="0F660ED22D5136DE" modified="2016-07-06" version="1">MQRAVDHVLAVTKEAVKMITFESLHNIVRLINGLSALLLAIMPGKASILEGMQGWELRPTVRGPRLPRWMENGASSFNHFIHELAVDSDECSSVDYSSEEDNSDDNFSPKTPLSQRSRVLRSTSFGRHDRRRGRRSRCFRFILAWLLFPARLMIGILFYIFRVSPSRSLNVANGSGNQQPKDSSTCKEASTLKDHIVQHATDKRRGVIEDLYLGIEIFIEAVFDLFHKAAHCLLSPLDTAKRLIRWFCFRRSKGKASCASKASVPTTILAEGDPAPTTRTVTFRDSLNTDTRTCRDVITELGYPYEAIRVVTADGYVLLLERIPRRDAQKVVYLQHGVFDSSMGWIANGVVGSPAFAAFDQGYDVYLGNFRGLVSREHVDKKISSQQYWHYSINEHGTEDIPAMIEKIHEVKMSELKSLKPNLEKDGEQPYKVCAICHSLGGAGILMYAVTCRIKEKPHRLSRIILLSPAGFHHDSNFAFKLLEYAFRWLAPILKPLFPAFYIPTRFFRLLLNKLARDLHNYPAVGGLVQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPGVSFLVVLHLAQILHNRKFIMFDYGCRSANIKKYGSPKPLDLGQHYGLIDLPVDLVAGRKDQVIRPSMVREHYKVMKDAGVKVSFYEFEYAHLDFTFSHREELLAYVMSRLLLVAPAPSPKSLKMKYTTSEIVEETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CFL2</accession>
    <name>A0A166CFL2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0314126</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CFL2"/>
    <dbReference type="Gramene" id="KZN03712">
        <property type="protein sequence ID" value="KZN03712"/>
        <property type="gene ID" value="DCAR_012468"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213306"/>
    <dbReference type="OMA" id="AGANECG"/>
    <dbReference type="OrthoDB" id="1904546at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05489">
        <property type="entry name" value="xylanase_inhibitor_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000045">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033868">
        <property type="entry name" value="Xylanase_inhibitor_I-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF22">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG94829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="434" mass="46744" checksum="42037275E5CB3FAF" modified="2016-07-06" version="1">MAPSLQITLFSLLFIFTITQAQPSFRPSALVLPVKKNASTLQYVTTIHQRTPLVSENLLVDLGNRYMWVDCETNYVSSTYRPVRCETSQCTMSNPDTCSDCFNRPRPGCNNFTCGVFLENSVNNIAMIGEVAQDVVSVRSTDGSSSGRVVSVPHFIFSCAPSYLRRNLSSGAVGVAGLGRNKIGLPSQFASAFSFKRKFAMCLSGSRSSNGVIIFGNDPYKFLPGIIVSDKTLTYTPLLTNPESTSASSHGEPSVEYFIGVKSIKINSKIVPIDTSLLSITNGNGGTKISTIDPYTVLENSIYKAVLKAFIKESAARNITRVASFAPFGACFSTDNILSTRVGPSVPSIDLVLQSESVVWTITGSNSMVYVNDNVVCLGVVKRGSNPRTSIVIGAHQLEDNLVQFDLASSRVGFSGTLLGRQTTCANFNLTSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CWN7</accession>
    <name>A0A166CWN7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_005275</name>
        <name evidence="5" type="ORF">DCAR_0205923</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CWN7"/>
    <dbReference type="STRING" id="79200.A0A166CWN7"/>
    <dbReference type="Gramene" id="KZN04438">
        <property type="protein sequence ID" value="KZN04438"/>
        <property type="gene ID" value="DCAR_005275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN04438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG86705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9370" checksum="59AB14D16C456354" modified="2016-07-06" version="1">MAIPEEPTINPNTLPHTLTAHKRAISAVKFSDDGALLGTSSADKTVKTWSPHTGALIQDFFGHDLVACSDDKTVRLWDLNATTPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FRJ3</accession>
    <name>A0A166FRJ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FRJ3"/>
    <dbReference type="Gramene" id="KZN08090">
        <property type="protein sequence ID" value="KZN08090"/>
        <property type="gene ID" value="DCAR_000759"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203265"/>
    <dbReference type="OMA" id="QQWITIY"/>
    <dbReference type="OrthoDB" id="1932208at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000222">
        <property type="entry name" value="casein kinase 1-like protein HD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="608" mass="67913" checksum="3BFBC5FF2B5D969B" modified="2016-07-06" version="1">MDELDSGGGQSGGDNNNKAVDADNDDGTTPIPDRVQVGTSPSYKVERKLGKGGFGQVYVGRRLNAPVPHERTGSGAVEVALKFEHRNSKGCNHGPPYEWQVYGSLGGSHGVPRVHYKGRQGDYYIMVMDMLGPSLWDVWNNKSNAMSTEMVACIAIEAISILEKVHAKGYVHGDVKPENFLLGPPGTPEEKKLFLVDLGLATKWKDSSTSQHVDYDQRPDVFRGTVRYASAHAHLGRTASRRDDLESLAYTLIFLLRGRLPWQGFQGENKGFLVCKKKMATSPEALCTFCPAPFREFIEHVVNLKFDEKPNYAKYISLFDGIVCPNPDVRPINTEGAQKLQVGHKRGRLALDEEDGEKPKKKIRIGMPATQWISVYNARRPMKQRYHYNVAEGRLAQHIEKGNEDGLYISCVASCSNLWAIIMDAGTGFSSQVYEISPQFLHKEWIMEQWDQNYYISAIAGANNGSSLVVMSKGTQYAQQSYKVSDSFPFKWINKKWREGFFVTSMATAGSRWGIVMSRGAGFSDQVVELDFLYPSEGIHRRWDGGYRITSTAATMDQSAIVLSIPRRKPADETQETLRTSAFPSTHVKEKWAKNLYIASVCYGQTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FWA7</accession>
    <name>A0A166FWA7_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FWA7"/>
    <dbReference type="Gramene" id="KZN08260">
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        <property type="gene ID" value="DCAR_001325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="26591" checksum="F846B2D437A0E2FB" modified="2016-07-06" version="1">MALSNWSAYNISALWSYARDGKRLSCLSQRNNTSIVGIVGASNLRINLGSDSSCWKRTTRFIRGSHASCYELTSIRSLQVSGRYGIHSLTEGMRYNVLIVVELLQTCITGPLTCTLIDSTGHRHDSKKYYCSQMVKNQIISLHVGQFCARKSLFGYGEINFSVVNTDKERKHGIVVIGALVVPVKSAAVAEAKESKLSTDDKEEGQLYESVEVCLLQPLQLQQTIHPLVMHYKDITISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GR01</accession>
    <name>A0A166GR01_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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</entry>
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    <accession>A0A166I3C5</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG84377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="304" mass="34277" checksum="01A75FE50ECDFC49" modified="2016-07-06" version="1">MADEEENEISDEQKIEIAKWFLLNSPPGEIQYVAKDVKAILGDDTVYESAASEAFPLYNKAHLISLQFPNRSGDVLITSYSEINANEYLDPRTAQVAKVDHIKQVCKEVRPATDEELPSSYVEEYRCAVEAELSKYVGEAYPKGVCSVFCTRGKDVEEPGSDFELVVVISAARHSPQNFCNGSWRSIWTIEFKDDLQVVEVTGRVQVGAHYFEEGNVQLDAKHECKDSTIIQAADDCAISLTNIIRHHETEYLTSLQASYSNLPDTTFKDLRRKLPVTRTLFPWHNTMQFALTKDITKELGIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IVV6</accession>
    <name>A0A166IVV6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_004203</name>
        <name evidence="4" type="ORF">DCAR_0104497</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IVV6"/>
    <dbReference type="STRING" id="79200.A0A166IVV6"/>
    <dbReference type="Gramene" id="KZN11547">
        <property type="protein sequence ID" value="KZN11547"/>
        <property type="gene ID" value="DCAR_004203"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207557"/>
    <dbReference type="OMA" id="WSSQGCG"/>
    <dbReference type="OrthoDB" id="4206278at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000029">
        <property type="entry name" value="Elongation factor Ts, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325:SF20">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN11547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="155" mass="16968" checksum="89B438478EEE78F4" modified="2016-07-06" version="1">MTIKIKGIYKGFRYITQIFVVKEREMEIGFPTDVKHVAHIGWDGQSGSSAPSWMNEFKTGPDFASTSVGNSGSAFSPWSSQDFGERLGRQTASDMFTDIPPTDMPNIPKKQKHKKQKSTSSPKSSSSSTRSSRAAKVKAKFLEGNVKASTSIEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175Y951</accession>
    <name>A0A175Y951_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Voltage-dependent anion-selective channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0624800</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Forms a channel through the cell membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin (TC 1.B.8.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175Y951"/>
    <dbReference type="Gramene" id="KZM80113">
        <property type="protein sequence ID" value="KZM80113"/>
        <property type="gene ID" value="DCAR_000295"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192390"/>
    <dbReference type="OMA" id="DKYPRFG"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000003">
        <property type="entry name" value="Outer mitochondrial membrane protein porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF23">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN PORIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0626">Porin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH05384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="277" mass="29546" checksum="9FA1F7F9C2BC673C" modified="2016-09-07" version="1">MSNGPGLFSDIGKKAKDVLTRDYISDQKFSVSAASDTGVVLTSTAVKKGGLSAGDVAAQYKYKNTLVDVKLDTESNIATTLTISEIVPSTKTIIGFKLPDYNSGKLEVQYFHRHATFTSAVSSNKEKHVVDLSATLGTPTFAFGAEAGYESALGKLTKYTAGISVTRPDSCASVLLGDKGDSIKASYVHHLDEIKRSAAVAEISRKFSTNENTFTVGGVYAVDHLTVLKAKLNNHGKLFALLQHEVIPKSLLTIASEFDTKALDITPKIGLSLALKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YB83</accession>
    <name>A0A175YB83_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </dbReference>
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        <location>
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            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="99" mass="11376" checksum="7D1BD35BD794DDC1" modified="2016-09-07" version="1">MYKHPNSTNTNTKCHHNPLSPLFSNRRRNNPPDFLLRPIINKATLTLALFLKYQQLASNLELCCHRQQTTQSLPPTTHTHSAHASFFHLPFSLNTTRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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</entry>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Yildiz M."/>
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF35">
        <property type="entry name" value="2FE-2S FERREDOXIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH16225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="158" mass="17355" checksum="5238CBA67A7E4A9B" modified="2016-09-07" version="1">MAISTINRLSKLSKSPSLYLSLRSTTTAVAAAASTGTKKVSDRIVKLFAIDPSGQKRQIIGLSGHTLLKALTNSGMIDPASHRLEDIDACSAECEVHIAQEWLQKLPEASYDEQYVLKRSQRNRVLNKHARLGCQVVLSQELQGMVVAVPEMKPWDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YJI2</accession>
    <name>A0A175YJI2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_030979</name>
        <name evidence="18" type="ORF">DCAR_0935943</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM83410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YJI2"/>
    <dbReference type="Gramene" id="KZM83410">
        <property type="protein sequence ID" value="KZM83410"/>
        <property type="gene ID" value="DCAR_030979"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201699"/>
    <dbReference type="OMA" id="IDVIVIW"/>
    <dbReference type="OrthoDB" id="630817at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053211">
        <property type="entry name" value="DNA_repair-toleration"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060">
        <property type="entry name" value="DNA DAMAGE-REPAIR/TOLERATION PROTEIN DRT100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060:SF21">
        <property type="entry name" value="L DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044632" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="15">
        <location>
            <begin position="29"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="843"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="16">
        <location>
            <begin position="31"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KZM83410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH16390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="885" mass="97919" checksum="A068B331ACCC0CEB" modified="2016-09-07" version="1">MAKPLSLIKLLLVHCFFMFMFMFMQCLSCPAHQKQSLLHLKSSLLELYETTNSTIFGLESWNSSSDCCAWNRVVCSLHSRDITALHLNILLPLDDYNYDVPFVDVTILDPIYSIRSLMLLDISENYMVGEISDTGLVNLTRLVHLSMSSNDFEGSIPAQLFHLRSLQFLDISGNLLEGGISGKGLANLTKIIHLDISQNRFYGSIPSQLFDLRYLQFLGLSANSLTGGLSSEVGKLGNLRTLKLGNNFLDGNIPMQIGNLTELQQFVVNDNKFWGQIPDTIGKLKGLEILDLGNNHFHKQIPSAIGNLLNISTLSLQNNSFNGVIPPSIRNLSKLETLHLEDNLLSGEIPLWLFDRESLKILYLGGNRLIWDSNVKLVPKCVLSKLSLRSCQVYGDIPKWIFTQKNLDVLELSDNHMTGEFPLWLAEMKIEILLLSRNKLTGSIPSSLFRSTRLSLLALSENNFSGELPKNVGDATNIKALMLSANNFSGAIPKSIADIRPLLLLDLSRNRFSGNTLPALNSAPLLHYVDLSSNELSGDVPVSFSHGISILALGENKFSGKLPKKLADMKQLVHLDLHDNNITGVFPEFLSCIPTLQVLILRNNSLHGSLPPNLFVNQSKILILDLSSNNLVGNIPSGLGNLMATFTTLYGYPYGTGDTGLWVNWVAGTVTMVSDMLSYTIEINDLMVTWKKSVRGISSHNRGIYSLLDLSNNKLSGDIPASLGNLRNLKQLNISNNRLSGHIPGSLGDLESIESMDLSTNKLSGTIPQSFGKLEQLSVLDVSNNKLSGKIPRGRQMDRINNPSYFANNSGLCGMQITVKCSGDEPKPGDNDDDDDVDEKEPWFLWTGLWIGFPLGLISSVLTAFLGGYFVIPTTKYHSIHFRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKA8</accession>
    <name>A0A175YKA8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde oxidase GLOX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glyoxal oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_028781</name>
        <name evidence="11" type="ORF">DCAR_0830537</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM83797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKA8"/>
    <dbReference type="STRING" id="79200.A0A175YKA8"/>
    <dbReference type="Gramene" id="KZM83797">
        <property type="protein sequence ID" value="KZM83797"/>
        <property type="gene ID" value="DCAR_028781"/>
    </dbReference>
    <dbReference type="OMA" id="PDPVWEM"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.80:FF:000001">
        <property type="entry name" value="Aldehyde oxidase GLOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
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        <property type="entry name" value="Immunoglobulins"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GO-like_E_set"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044662" description="Aldehyde oxidase GLOX" evidence="7">
        <location>
            <begin position="21"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="8">
        <location>
            <begin position="38"/>
            <end position="418"/>
        </location>
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    <feature type="domain" description="Galactose oxidase-like Early set" evidence="9">
        <location>
            <begin position="427"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00077505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM83797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH11058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="531" mass="58306" checksum="7F5C98E984E04894" modified="2016-09-07" version="1">MASLLFLTIVLVSISCFTRADLPGTWELLLENAGIASMHTAVTRFNTVVLLDRTNIGPSRKMLRKGHCRVDPNNPNKHDCYAHSVLFDLVTRSTRPLMILTDTWCSSGQFLPDGTLLQTGGDLDGVKKFRKFSPCKFSEFCDWQELENVELSHGRWYATNQILPDGTIIIVGGRSSSSVEFYPPRETGAVLFPFLAQAGDNQMDNLYPYVHLLPNGHLFIFANNKAVLYDFVINNVVKEYPDLEGGPRNYPSAGSSAMLALSGDYSAATVVVCGGAPYGAFIDRSTDTPAHGSCGRIDPTRPDPVWEMEDMPFGRIMGDMVMLPTGEVMIINGAQAGTQGFDLASNPCLYPVLYRPDQPLGLRFMTLNPGTVPRMYHSTANLLPDGRILLAGSNPHYIYKFGVEFPTELRIEAFSPEYLSPDRANLRPEIVELQETVKFSEVFDMFVTVPLPVVGLVEVNIASAPFATHSFSQGQRLVKLAVSSAIPDGEGLYRISCTAPPDGKVAPPGYYMVFAVNQGVPSVARWVQVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YNN8</accession>
    <name>A0A175YNN8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0831610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000077755">
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        <property type="entry name" value="GOLGIN FAMILY A PROTEIN"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="72"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="22933" checksum="9ABB7DC70F1F97DE" modified="2016-09-07" version="1">MEGVGARLGRSSTRYGGPATVFTGPVRKWKKKWVHIAPSNAASSNPRHSHSFTNANANGVTHLKLFKWTPLLNANAPDPDQTDDDEPPRRKFKYVPIALLEEQKDEASEQVEDEAAEQVEDEAKPSETNIVATDLNSKADDVDEKPDINNVPMEENETPENPLGRQDLNESTVDLGINLNSRDGENDSDSMNDQIKDGEPETLDSVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YSU6</accession>
    <name>A0A175YSU6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0833086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_026566"/>
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        <property type="component" value="Chromosome 8"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS09G0436400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389:SF4">
        <property type="entry name" value="OS09G0436400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH13576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="645" mass="72943" checksum="DF7C5BCF8F13356A" modified="2016-09-07" version="1">MYGFSSSSGPGYSPAAPPYYPYNYSGYYEPGPELCLRFPDKRESESLDARFDQSPVLKNDQYVKTTAENKKLWRIYSKITPSVVSVSSFLGVERKFDCSGLIIDWNSIDNEATVLTSAKLLWSPKDTSLEFHLIVRTADGTLFLAREDYVDYHHNLLTLKIKSTIELKVVDLRCRQAEYVERMSVIALGRDFQTCLPFDYVGELSLAFPNYGCDELLMSTCRFSEICEGGPLITDTGYVIGINFKDAHSLPAPVILSCLEMWSTVVRPWFGMSVVDVDQLSYCIWERYNIPVEGSYVVVKEVLKGSIADRNDVRHGDLVSTCNGVSIRSAKQYSQLLSKASQVLSACADSSQQSFTVVINPFDRHTDNISIEAEQVSVDDKSWQGILSGEWNKIDGLLRNPRRLRYDPTVPHYVPGESMYYPTVPRYVPGESMYYRTVPHYVPGESMYDPTVPPEILHNLMYGYSPTCPSYSPYDYSRDYEPGPELCLRFPDKKETGSLDARFDQSPLIVRTADGTLFLAREDYVDYHYNLLTLKIKSTIELKVVDLRCRQAEYVERMSVIALGRAFQTCLPFDYVGELSLASPNYGCDELLMSTCKFSELKLAAMHYSNRNKGEVKRKSLVAGFLLNPTVVKCGPLRKTPSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W118</accession>
    <name>A0AAF0W118_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G protein gamma domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100488</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="103" mass="11440" checksum="FBA39BB34A442C7C" modified="2024-05-29" version="1">MDPSTAQGDEGENNNRAALQSLPNFVGKHRMTAAIALLNQQIQFLQEEIDQLETLGGSSVVCKELVASVESVSDALLPVTKGPTEVAWERWFQRSSKNRKKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0W2G7_DAUCS</name>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="2">Glycan metabolism.</text>
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        <subcellularLocation>
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        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
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        <property type="entry name" value="O-FUCOSYLTRANSFERASE 38"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="48"/>
        </location>
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</entry>
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    <accession>A0AAF0W396</accession>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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            <begin position="21"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="68" mass="8048" checksum="6BEFDC1C44FC126D" modified="2024-05-29" version="1">MFMLLHICMYFLLRINSAHKELFNQSVKGLDQTKINIHKRLLRANTNDYGSYDPAPTFHKPKFKQIPN</sequence>
</entry>
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    <accession>A0AAF0W3N4</accession>
    <name>A0AAF0W3N4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3 19">Peroxidase</fullName>
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    </protein>
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        <lineage>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 19"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3N4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000010">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="7 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041766283" description="Peroxidase" evidence="19">
        <location>
            <begin position="24"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="24"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="34"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="100"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG82625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="307" mass="32986" checksum="1A6F4D32F43DD1A0" modified="2024-05-29" version="1">MDSFLKQVSVLVLLLLVTPSALAQTKVGFYSTSCPKAETIVSDTVKKHVKANTAVAPGLLRMIFHDCFKTATPNLLLRGYDVIEDAKTQLEAACPGVVSCSDILALAGRDSTVAVGGLTYKVPTGRRDGRVSLASDTANLPSFRDSVDVQKKKFSDVGLSAQDLVTLVGGHTIGTVACQFVSYRLYNFTSSGGPDPTIDSTFLSTLQSLCPQGGDGNKRIALDYGSGDDFDNSFFKNVQNNRGILESDQSLWQDNTTQTYVKRYIGIRGLAGLTLNVEFAKSMIKMTNVGVKTGTDGEIRKVCSKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4W3</accession>
    <name>A0AAF0W4W3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0102447</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4W3"/>
    <dbReference type="KEGG" id="dcr:108225918"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF33">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="25"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG83272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="434" mass="48115" checksum="5961D42E83F47747" modified="2024-05-29" version="1">MNRTSSSLRRTSSSSGSGGIILEKYQLGQILGRGSFAKVYRAQNLGDNSSVAIKIIEKPAKSDPTMENLLVREVAAMRKLNDHPNILNLHEVMATKSKIYLVMELAEGGELFAQISRRGKMNESVARRYFQQLISALRFCHANGVAHRDLKPQNLLLDKQGTLKVSDFGLSALPDQAKNGLLYTACGTPAYTAPEVVRRSGYDGAKADAFSCGVILFVFLSGFLPFDDANLGNMYKRIYKREVVFPNWISKGARNVIYKLLDPNPNTRMTMEGVMSTNWFSKSLKVETEVDKLGVLLGKDCKNDGSVNAFDIIMMSSGLDLSGLFEADLSKKERRFTSRGSMKEIEERVGRVSEELGYKMEKGKGREMGLVKGKIILLVRILELVTKMGLLMVEMEVISDGDEVEKGCDQLEQFKVGLGDIVVSWHNNDVVVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5P0</accession>
    <name>A0AAF0W5P0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="7">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0101977</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the CCR4-NOT complex, at least composed of CRR4 and CAF1 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="7"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5P0"/>
    <dbReference type="KEGG" id="dcr:108212192"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000016">
        <property type="entry name" value="Carbon catabolite repressor protein 4 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF79">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4 HOMOLOG 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="20">
        <location>
            <begin position="212"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG82809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="590" mass="65803" checksum="0A89738BDF37052E" modified="2024-05-29" version="1">MGAGDFVGPEVEIEVVMSLRSKTPVVGCEINPSVRVSGHGSGQPPPHTLNFTWYRERSTAATDAQEVCSLESHLSVTDASDINPLRPIDEMAQKNNPATNPLHAAGNVLPLDLSYHNVIDVKREGITWIKLGSSRSYVPSSDDVGFALKLECVPIDHSTGVHLSQVFVVLTNPVIIFPAPSPRSMISVGSAKKSECSSIEPQSISGINFSVLSYNILADLYASRNAHQNTPAWALTWEYRSQNLLNELIGYHADIVCLQEVQSDHFDTLFQPEMERHGYSSVYKKKTKEVYTANQYITDGCAIFFRDDKFKLIIKYELEFDNHLFPVVDMLPPNQRNEVCFRLMKGNIAVVLILEAVGNDSMQNGLKPRIGVANTHIHADTNFQDAKMFQVVNFIKGLEEISNSQIPLLICGDMNSLPKSDPYKFVREGKVRHVSNKLRDPLGIQKHMVFSHSMRLASAYTSLGQSVSVDGKQKAKMNSKTGEPKFTNFGSGHPRTLDYIFYTEDDLKVEELLELLDKRNIGGHALPSPLWSSDHIALMASFSYKQGYISQQLSVPRNPWEGKTPGLKLLLMEEFGEGNEYTSRHCKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5R2</accession>
    <name>A0AAF0W5R2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0101365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5R2"/>
    <dbReference type="KEGG" id="dcr:108195421"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG82203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="231" mass="25467" checksum="D010B129A127491E" modified="2024-05-29" version="1">MEKTNHHPEHQHQNPPVNHQPPPVFFPPLGAARGPSFPPNEQLVQLNYCIHSNPSWPQTVLLAFQHYIVMLGSVVMIATILVPRMGGGPGDKARVIQSLLFMSGINTLLQTFLGTRLPTVMGPSFAYVISVFGIINDFSDSNFSSEHQRFLHCMRSIQGSLIVSSFINIILGYGRVWANLTRLFSPVVIVPVVCVVGLGLFGRGFPQLANCVEIGLPMLILFITVCGFKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W650</accession>
    <name>A0AAF0W650_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0101476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W650"/>
    <dbReference type="KEGG" id="dcr:108209986"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17058">
        <property type="entry name" value="Ubl_SNRNP25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039690">
        <property type="entry name" value="SNRNP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040610">
        <property type="entry name" value="SNRNP25_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942:SF9">
        <property type="entry name" value="OS02G0188500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942">
        <property type="entry name" value="U11/U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 25 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18036">
        <property type="entry name" value="Ubiquitin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="251" mass="28883" checksum="C359BEE6DCAAF564" modified="2024-05-29" version="1">MHETKDESAQRRSSFSCAVPLVIIDSFSRRSFSYNKLPQNPLKLTVLKLDSSSFEIEVSKTATVAELRKSVENAFSHLPKEGPGKISWSHVWGHFCLCFDGQKLLDDNDYIADYEIRDGDQLLFARHLSINYNLKRNISTDQIGDIEQPSISNTWEPEEVIDERIYESCDDETPRQKVEEHTFPDIPTFEYKLATILRGWFSYRRVSSSPEPNFNSKSISRSSSGFLGSFKNVIMGYNNKQIPRIENLKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6M8</accession>
    <name>A0AAF0W6M8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA polymerase</fullName>
            <ecNumber evidence="13">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0101819</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="13"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000014">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000082">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="19">
        <location>
            <begin position="10"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="18">
        <location>
            <begin position="64"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="16">
        <location>
            <begin position="1069"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="15">
        <location>
            <begin position="1340"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="17">
        <location>
            <begin position="1809"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG82653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1910" mass="212724" checksum="0760CEC79E86D3CE" modified="2024-05-29" version="1">MEESDKKKIFSVRIVSIDYYMSPPITDMDISYSSFQGGRVKEVPVIRIYGSTPAAQKTCLHIHQALPYLYVPCSELSLRPELEGEACMHSLCLAIEKALKLKGNAGSKRQHVHGCSVVQARRLYGYKSSEEFFVKIFLYYPQDVSRAANLLVGGAILDKVLQPHESHIPFLLQFLIDHNLYGMGHLHVSKMKFRTPVPDGSQSEAKGNRHHRQKTDSSDCFPVDSLVESSGNACFGLPLWTSLTIPDGWLWQQSSQLDPSIGPNPYIISRQSTCQLEGDASVHEILNQQNLSYSSLSQTGSDVKFVQSLIPIWEEFEKAGMHGVASLSIPGKPLPEDTLRILSDGGEFVDKYMELFSNTSTQIKDHITIFSDDANVDESNRVICESSPYSKAKNSAGLDLCAEKLLADKVLSTVGPSNVKSADYEAEALLKWLASSQAAEDINSDDELARETILSPLLPASTMVKMLERANIDYESQSQQECQDILDCVEDSIDIVASKRKESRPIDDCQLPLKENITRAEGSNEERILISCSAKSSEIETNSEFEKSLQSYTPVDFGSGFTTIQKRKRPLQRSGSFSMDQKLEGDIHPEISNENCFGVNNEIKRRNVATGCEENDLECSEETSVNIKSSMRDLMRRKRCYQVGLPECDTSHERVSIQEELKKDASLCIEDNSEDLKHRERNFSQQTAIVVRRTCEAQPKSGAAPVEPDNFRYGILPLLSHSDHSGPLTITADGSCFGDINDPKVTAPTARSGNRYLGSMAIHEHTKIASQLVDSENQNSAAELGSFENKTGEDLKDVSKIVTPLSFSRSDPCCRKLNFDDDILLETEKVLPTAAVCSSQPCTLQPQNIVFDNHGHFSGSHSTNPLQREMEVSAENPSGLDLRITHINTRCSSKRNEDQNTRRIAEDFNYHQKGVMGIPAHFYNDNSSIYILTPAISPPSADSVDRWLSQDDTVIPNRNRFFKLVRFIASSFGILTDSHSSLADARRDLGPHEDAIIAHVKQPIEESHTAGLRPLHSDSTKMPNNDVTTLIIEQNTCYAEDTSQISAPDGKSKLTPTSQIGFQDPASAGGSQQLTIFSIEVQAQSKGDSRPDPRLDAINFIVLVIFEDDGSLTETHVLLHSDTAPAPRNLDGISGCKMLVFSEEKELFNHFIKFICSYDPDILMGWDIQGGSLGFLAERAAQIGIGLLNKISRTVSQTKELGQDSDVRVKSVITDALLESTVADSAVLDDAIIEDEWGRTHASGIHVSGRIILNCWRLVRSEVKLNMYTAEAVAEEVLRQKLPFIPCKELTKWFSSGPGRARYRCIEYIINKAKLNIEIMTRLDMINRTSELARVFGIDFFSVLSRGSQYRVESMLLRLAHTQNYLAIAPGSQQVASQPAMECLPLVMEPESGFYADPVVVLDFQSLYPSMIIAYNLCFCTCLGKVTPAKSNTLGVSSYSPDPNVLMDLKDEILLTPNGVMYVPSKVRKGVLPRLLEEILSTRIMVKQAMKKLAPSERVRERIFNARQLALKLIANVTYGYTAAGFSGRMPCAELADSIVQCGRRTLETAISFVNAHEKWKAKVLYGDTDSMFVLLRGRSVKEALEIGQDIASTVTAMNPNPVTLKLEKVYHPCFLLTKKRYVGYSYESPYQVKPIFDAKGIETVRRDTCGAVSKTMEQSLRLFFEHQNLFKVKHYLLRQWKRILSGRVSLQDFVFAKEVRLGTYSSRASSLPPAAIVATKAMKNDPRAEPRYAERIPYVVVHGEPGARLVDMVIDPLELLAINSPFKLNGFFTTKMPRPEQKAFGKHQLNALNPNRTRIDSYYLSKHCILCGELVQASTYICGKCSKNEAAVATAMVGRTSKLEREIQHLADICRHCGGGDWIIESGVKCTSLACSVFFERRKVQKELQSLSVIAADADFYPRCIVEWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W951</accession>
    <name>A0AAF0W951_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Protein DEFECTIVE IN MERISTEM SILENCING 3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0103182</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84003.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EN/SPM-LIKE TRANSPOSON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33566:SF6">
        <property type="entry name" value="PROTEIN DEFECTIVE IN MERISTEM SILENCING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="47842" checksum="66FEA5F6395F2AE7" modified="2024-05-29" version="1">MSACNTGTPKAEIDEAYQQAEAAVRVTKKFQDDIEILGLTVKHYEDKIRYLRMEKDGVDDSIIDLEVITGKHHASSIKPEEEDSQSEDETYKNILKLEKSAAGLVCQLKTRHGTQVSHSPLVNDVTGIVATLGKVDDENLSWLFAEYLGKETMLALVCKTFIGVEAIESCDTEGAINRNHGLHGLGYSVGRTIHGRFHVICLADIIPYVGKFIANDPQRRLDLPKPKFPGGEIPSGFLGYAVNMIHIDKANFYVTTSGYGLRETLFYHLFSHLQVYRTRKDMLKARPLISDGALSLDGGIMRSRGDYYLGNREEAEVKFPRICEKSSVPGNYYEIENNLHRKKWDLERLLEVMQREQSKLDQAKFDFEIKKQDFVRFLARSSHPAQVLMRGNLKVHFPPTDSHYKSGLMTSFSSVEFICIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9T8</accession>
    <name>A0AAF0W9T8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0104051</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9T8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="hAT-like transposase RNase-H fold" evidence="9">
        <location>
            <begin position="456"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="8">
        <location>
            <begin position="611"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="81183" checksum="2D4A38C01CD16556" modified="2024-05-29" version="1">MAPSKRKKNHFEVIPENAEFEQLRSLGDESESEDVGEIGDNNDISEQQQFQVPEQEEISRKRKSGSHETQQSQPYQKKSRKKKARCWAYMDLVEENQQKSAICRFCKQKMKCDATGCTTTLNRHVDKCLKSYGQTSQALLQFQPSDTKSTEVTLGTFKYDHAEMRKVISHYILVNELPFRHVESFMFDKVMRTATPFWEKISRHAVRKDCFSTYEIEKTKLRELFKLVRKINITTDMWTSSHQKIGYMVVTGHWIDADWRLNMRVLNFCNVPPPHSGFIISEALFKCLDEWGIVDKIGTLTVDNAAANDVALRYLKQTFSVRRSLPIEGKMFHARCCAHILNLCVQDGLDPTEEIVDKVRDGVKYIAASEGRRIKFAEISKSLGLKCKKLILDVSTRWNSTYNMLACAIEFKNVFLIYSTSDQGFKEYVPSEEDWERVAGVCSFLEVFSDVTKVVSGTGYSTSNLFLSEIRRVKQIIDKKAIDPNLHIREMARKMELKFEKYWGETNLVMAIGAVMDPRFKMKLPAFCFPTLYPIASDSETNLSYLGNALTDLYLEYCREEKDASKEKNESGISSSDANFMAEHSETPQGINDYESFIRESGGILEPTKSELEEYLSEKIVAPTSKFDVLAWWKGNSSKFPILSKMAADILSIPISTVASESTFSAGSRVIEPHRSCLKPETVEVLLCGADWVRELYGLKKAKQVNGYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WA56</accession>
    <name>A0AAF0WA56_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0205368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WA56"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035266">
        <property type="term" value="P:meristem growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044622">
        <property type="entry name" value="PCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45086">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN PCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45086:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN PCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="91059" checksum="61E258B5C0454753" modified="2024-05-29" version="1">MARLQYKSSDYKPPKQYKSSSIDYKPSPIVALATSPDDSRVAAAREDGSLEIWLVSPGAVGWHCQLTIYASPNSRVSSLVWCHSNGYSLGRLFSSSIDGSVSEWDLFELRQKNVLSIGVSIWQMASAPYKNPHSNEKKHPQYIKMISDSSDDDDCSESDNDIDSVVTTDGSIVEDANVAMACDDGCVRICTVSDEFIYHKSLPRVSGRTLSVTWSQDADKIFSGSSDGLIRCWDFKTVREVFRITVGLGELGSGPELCIWSLLSLRSGTLVSADSNGRVQFWDSQMGTLLQAHSRHKGDVNALSAGPSHNMVFSAGSDGQVILYKLSRDVSGSTDKKSSVRVIEKWVYVGYSRAHTHDVRALTVAVPISREGDVYRKRKKDRVKKPHDFSFRKWGHIGVPMLISAGDDTKLFAYAANEFTQFFPHDICLVPQRPPMQLVLNTVFNQSPLLMIQASKWLDVFCVQSKTSSHPDTSSDPCAGIATTDMVVRVKSKASQKIICSSMSNSGVFFSYSDEKKPSLLKLNKIEAQKSAWTIEKRKLPNLPFAHSMCFTYDSSWLMIAGNDRKIYVVDVESLILICTYTPRCKDIDDEILSEPPLTRMFTSPDGQWLAAVNCFGDIYIFNLEIGRQHWFISRLDGASVTAGGFTPRNSNILVISTSSNQVYAIDVDAKQLGEWSTRHKFTLPRSCPKFCGEVIGLTFPPSLNASSVIVYSPRAMCEINFETPVIVKDLDAPVRKLPSSPSKKELKRKLKGCDFEKKVEKKTKHNGKEDFEITPFDEPVLFAGLLSKGSLFVMEKPWSRVITKFDAPPVHSHIYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAA5</accession>
    <name>A0AAF0WAA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0103508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAA5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025452">
        <property type="entry name" value="DUF4218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004252">
        <property type="entry name" value="Probable_transposase_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004242">
        <property type="entry name" value="Transposase_21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10775">
        <property type="entry name" value="OS08G0208400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10775:SF182">
        <property type="entry name" value="TRANSPOSON, EN_SPM-LIKE, TRANSPOSASE-ASSOCIATED DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13960">
        <property type="entry name" value="DUF4218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02992">
        <property type="entry name" value="Transposase_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03004">
        <property type="entry name" value="Transposase_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4218" evidence="3">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="645"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG84325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="692" mass="79978" checksum="5A0B9CA53EA31C13" modified="2024-05-29" version="1">MLSGWKTAGHLACPHCAHDHDAYNLKHGGKTTWFDNHRKFLPRNHPFRKNKNWFTKGKVVSEFPPPIRTGEDVLQEIESLGLMKITELGSEEHNARIIKTYNCGWKKRSIFWDLPYWRTLSIRHNLDVMHIEKNVFENLFNTIMAIEGKTKDNAKARADIALICRRPELAINESTQKYPKACYSLDKKGKEAVCKWLQDLKFPDGYVSNMGRCIDMKKYKLFGMKSHDCHVFMQRLLPIAFREFLPNNVWEAVTELSLFFRDLASSTLKVSDMCRLEEQIPVILCKLERIFPPALFDSMEHLLVHLPYEARIAGPVQYRWMSARRLRLTSSPNPGNSTEHTPIRSQRSQTEHTPAQSRVLSSASANEPEHASESEDPYWEVGSMHDDGRMRVEVISGLLEPSGRCSRAITDSISERQDPDGFSWKAVSKEVKDFYFDEFKKTCVWRQDDRIIHKAWAKKASVRYTNFCSDARARWEKGGQDSRVAMHVWLRWVEFWKTPNFQVKSNTQKKNRKGGADKYPPTHTGGSASLRTHAAVLAKTQGKDPTPADVYLITHTVKHDKKTFVTKKAEQVYKRVETLREELSAPIEGSDEPQVVDEDQLFLKAAGGLDKKSRVYGMGSLQSVIYGPETGGSRHRGSNFRHEEYKQMQVELQDMKNQVKELQEMRNQELEEMRKQMEEMKNQLALVFNHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCY6</accession>
    <name>A0AAF0WCY6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0206865</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SufE family.</text>
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    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
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        <property type="entry name" value="SUFE-LIKE PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43597">
        <property type="entry name" value="SULFUR ACCEPTOR PROTEIN CSDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S metabolism associated" evidence="3">
        <location>
            <begin position="77"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG88721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="219" mass="24432" checksum="4033FEB5547765AA" modified="2024-05-29" version="1">MYSLVSQSSSPPSLTSSSSIPSICMNKHIQINPRNQNSRKHKPFSIKCKHPICSPRIINPSVNSSDNVARKVNCLIAEFKSLKEPIDRVKTLLHYATLLPFLDESIRSEENKVPGCTAQVWLEVKMDFEGFMRFRVDSDSEITKGFASCLIWLLDGASPEEVVAVKAEDLAEMNVGILPANSRVNTWNTVLLSMQKRTIALAEQRFGDSSKSLALFTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHZ9</accession>
    <name>A0AAF0WHZ9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0209503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 11"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG90260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20180" checksum="EBF441B7E3DB8018" modified="2024-05-29" version="1">MAKVYPCTPSSSSCCSGSSSGKTYTIWMKSLVANGNGFTVYDSCGRVVYRIDNYSKKSCRQVYLMDLHGNVLFSMFQKNEKPFFEVKNCRKFLKRRETEYHVTMGSDSCSFYKIKGTEGKCEFNITDQQGRVVAEATQKQSSSGVMLGQDVLSLKLEAHVADHSFIMALVAVHGLITRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNP3</accession>
    <name>A0AAF0WNP3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNP3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="43271" checksum="ED2078BED8E58C18" modified="2024-05-29" version="1">MIFHLKDEQRKWVHRSGFGLLLNFELEMLPAKLAYNVLQIFDHNSVSLKLKSLDIQITKDDVFDVLGLPYGGLKIQLADETKFKQREECWNAQFSTEKKEREQITAQMLVQKMRKQGVSDNFKLNFLIVMSNALIGTTSSSYVDKQLLRIDDDLDHLQRYNWSEYLLHYLVIATEAWNRTASTFFRGSLVFLTNLESNQDTDIAKSSTKLPVAKNNQDVIPSFSLGIEELDDLRNATVFFPITKSEHFYLICYDIRNQGHFIIDNIKREGNPKQYYMRVPDILHSHFCNYIQIKGNIPLSRRIRRFKRTYLTMPWQTSWNSTDCGIFVMRHMETFKGDPKKWDSGLAEEGVSDLNNYLAYCNIHPHVPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WP95</accession>
    <name>A0AAF0WP95_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0312555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WP95"/>
    <dbReference type="KEGG" id="dcr:108210592"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000455">
        <property type="entry name" value="Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="48870" checksum="92B2E862CEF52084" modified="2024-05-29" version="1">MATQTPASPPYDIIIFGASGFTGKFVIKEALKFLNTPSSPLKSLALAGRNQSKLTQALSWAAHPNPPPSIPILTADTTDPSSLDRIAAQTKLVLNCVGPFRLYGEPVVAACVGNGADYLDICGEPDFMERMEANYYDQAVKNGSLVVSACGFDSVPAELGLMFNSRQWVLPAVPNRVEAYVSLESDKRVVGNFATYESAILGIANAAKLQELRRSRPRRARPTIPGFAPPKGSTIEHQKDIGLWAVKLPSADATVVRRTLTTLTENPHGLQGANESAEQAEKREKFWSTIKPAHFGVKLGSKSFLGVLRFIGVGVMMGLLGSFAFGRSLLLKFPSFFSLGWFRKNGPTEEEVAAASFKMWFVGQGFSDSSLASGKTKPDMQIITRLTGPEIGYLTTPIILIQCALVLLKQRDDLPKGGVFPPGIVFGPTELQERLQENGISFDVVSKSSLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPY5</accession>
    <name>A0AAF0WPY5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0312497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPY5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02043">
        <property type="entry name" value="serpinP_plants"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF340">
        <property type="entry name" value="SERPIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="23"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="401" mass="45175" checksum="1B701D8C56B3C037" modified="2024-05-29" version="1">MENSEQINTCDLNSDFCIQIANHALLEEADKASNFVISPVSFQIILSLIATGATGRTLDQLLSFLGSKSIDDLNSLSSKVVEITTRQVGDDNNLAASPLVTMVNGAWIDKSFGLKPSFKGTLTDVYKAEARAVDFATRANEVTEEVNKWAQDATKGLIKELLRSGCLGTDTALVFANALYFKGAWDRKFDSERSMNRDFKLLNGQIVQVPSMTTKKRERNLYREINGYKILKIPYQNGEDTRKFSMYFFLPQEINGLKSMIQTFNSIPGFFNQDFYFREEDLGDRIWIPKFKFSFEFEASRIIKELGLVLPFMDGAEFTELVHSLEGEKVCVSSIFHKAYIEVNEEGTEAAASTAVRFRRCCGRVKPPGFVADHPFMFMIREETSRIVFFTGAVLNPLLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQ31</accession>
    <name>A0AAF0WQ31_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein WDR12 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0312530</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WQ31"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000399">
        <property type="entry name" value="Ribosome biogenesis protein WDR12 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="12"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="204"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="271"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="357"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG93249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="436" mass="47904" checksum="9F79FD6AA869528B" modified="2024-05-29" version="1">MDIEEDEMSRQVQVRFVTKLKAPLKAPNTTIAIPSNLTRMGLSTLVNTLLQSEAAEWKHEAFDFLIQGELVRMSLGQFLLAKGITAEKVLEIEYIKAVIPRKEEEPSVHDDWVSAVDGSNPGFILTGCYDALGRVWKGAGICTHILEGHNDAITSVSIIKSKGARSSNDTLVATASKDKSIRLWKFDAEDTLDRPMTIRSFRTLRGHNASVQTIAGQASGDMMCSGSWDCTINLWQTKASEAGGDLVSVKKRKTGGENEESQSEGEAVSSFVGHTQCVSSVVWPEYGTIYSASWDHSIRRWDVETGKDTSNMFCGKVINSIDVGGEGSALIAAGGSDPVLRIWDPRKPGTLAPVFQFSSHSSWISTCKWHDKSWFHLLSASYDGKLMLWDLRTAWPLAVIDSHEDKVLCADWWKGDCVVSGGVDSKLRVSSEISVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWQ7</accession>
    <name>A0AAF0WWQ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyruvate kinase</fullName>
            <ecNumber evidence="4">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416378</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WWQ7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000051">
        <property type="entry name" value="Pyruvate kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="13">
        <location>
            <begin position="269"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="13">
        <location>
            <begin position="473"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="753" mass="82184" checksum="47B44E2748B015F6" modified="2024-05-29" version="1">MHIKTPLRCVFISYSSLATAAFGFSNFTSSSLCKEFAQLPICFDAKKPICKQKFVQLLAAQEGNLSRTITACSTSGNNSEPEESRTTACTNFKVLADTEKCESSRDVGDKFVTSILYSDADANFKAGNLGNQDNLFDKLKAVHLHILAMEQWNASRLKLCHRTYLVSAANLIHYVALKSLDIGQLKADLSSLGLLNLETINPHVLASLSAGIQMLENLKSNSFASKTSGEISNSKILSIQEKKEFTVNRMAKREFDRSKLLLGSLREQRTSHIMVTVGEEAAESETLITDLLKTGTTIIRINCAHGNPATWSEIIRRVKQSSQMLEKPCRIIMDLAGPKLRTDKLKAGPCIMKISPKKNAAGEVIFPSQIWMSPQGGGPPPSHASPDAVLYIDGQEFLSKLEIGDNVTFCDARGKKRSLRISNKYSVFAGVGLMAECKNTSYVKSGTILYIKGKKSKSAVGFVRDVPAVEQFVRLRVGDLLVISRDSLNEHEISSSNIGSPRVTCSSGYLFDSVKPGQRIAFDDGKIWGVIKGTSISEILVSITHAGPRGTKLGSEKSINIPESDIQFEGLTSKDLIDLDFVATHADMVGVSFVRDVQDIIVLRREIEKRKLENLGIILKIETKGGYKNLPLMLLEAMKSPNPVGVMIARGDLAVECGWENLADIQDEILSTCSAAHVPVIWATQVLESLTKSGVPTRAEITDAASGRRASCIMLNKGKHILEAVSTLDTILKTSSTELSPEVKPLMLSSHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X2F6</accession>
    <name>A0AAF0X2F6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0418379</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG99033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2F6"/>
    <dbReference type="KEGG" id="dcr:108218965"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000446">
        <property type="entry name" value="Microtubule associated serine/threonine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000147">
        <property type="entry name" value="probable serine/threonine protein kinase IREH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058783">
        <property type="entry name" value="IREH1/IRE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF354">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE IRE4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26031">
        <property type="entry name" value="IREH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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            <end position="939"/>
        </location>
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            <begin position="940"/>
            <end position="1037"/>
        </location>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
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            <begin position="622"/>
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        </location>
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            <begin position="816"/>
            <end position="840"/>
        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the QWRF family.</text>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04484">
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            <begin position="14"/>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9Z5</accession>
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        <lineage>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9Z5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF76">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="116"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="35329" checksum="E75C989A081DE043" modified="2024-05-29" version="1">MIVNKDESSLWSSWVLRTIIRGKHFWALSIPFDCSWIWRQVLTLRDKARSHISYDIGRGDSISLWFDPWFRATPLATGNSDPIIAYTYSTTGARVIHPSREDRILWDGRAWPLVNVRWLWESLRDHRDVVAWWKAVWFKHGIPRFRTHSWLLCLGRLGNLDRLINWRISTTPFCYLCVGGKENHNHLFLHCPYASFVLTHLLRPIVMVTIRPNWGWTDLLLCLMGVESPTRRLLSLLAVQIFAYHLWRERNARRHGKGVFGPKKLLHGIITDLRARVTSEPWFTAAMITSPQLQTCISYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAJ1</accession>
    <name>A0AAF0XAJ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0622936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAJ1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="46"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="695"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH03537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1147" mass="126233" checksum="F2DC1E945FB95EE2" modified="2024-05-29" version="1">MADSTHCVKLIESFVDSSSTPAQQAASIDAIAALVKNDMLTLESLVREMAMYLTTTDSFLRARGTLLLGELLACLASKPLESETVLSLIGFFTERLADWKALHGALIGCLALLRRKGNVGVVSSSKALAVAQSYLQNVQVQSLRQSDRKLCFELLGCLLERYPDDIVHLDDVLIPGICESIDGEKDPECLMLAFHIVEVLVHLYPDPTGPLASFATDLFENLSCYFPIHFTHPKGEDIDVKKEELSRTLMVAFASTPLFEPFAIPLLLEKLSSSLPLAKVESLKYLSYCSDKYGAARMAKHVKVIWSALKDIIYTSPESVLLLESEIVDGMSFQESHTVTEALILLQKVFCQNSDLLIDLIVIDEDIKKTVDSIYQFKEYTGIPLADKQRLHAVGSILYFSAKSSIGSCNRVFEAFFPCLINALGISAENSVALLSSPCNFGATYLCVELLAGCRALVVRTKGNATSNVIVQEAWCDILCTYSTSLTKLFSFTLITGVDGSTQNACLHSGVKGLQILATFPGDFSPISKSVYESILLKLISVVTGNCKDAFLWKLALKALVEVGSFVDKSEDTEKAQSFNAIVVDKIASFMLCDDLSMPLPLKLEIISSIGTTGLSYMQKFLQGLECSLLASLSGVYVRGDPENLEIATSLLECYSCDVLPWFDSIGGQEEVQFHFALTIWGQIGNILSFCASSQEQKLLSAAMTAMQYAVGRCLAENQTVIIDRAFGILLSTTSLPINDLMDEITSVKVEGYKSNDDLGCMSCREKWIISLFASVVAALRPQAHIANVKVILRLFLTTQLSGHVPSAQALGSIINKLPVKVENMDASNVFSLEDAIDLIFSSRVWSLCNEGPTNCSVARNNNETSIRNNLLIQSHAIEGLAWIGKGLLMRGHEKVKDLIVTLLSCLLSNHSEGSLPCTEQEFLCVMRSAADAFHILVSDSEACLNKRLHATIRPLYKQRLFSIVLPIILSLISKADSSIQRAMLYRAFANVVSGTPLSAVLSEAKKLIPILVDSLFVLSEDILNKDIVYCILLVLSGIMTDKKRSEVVEENAHIIVRRLIALMSYPHMMVIRETAIQCLTAMSELPHARIYPMRTQVLLSLLKTLDDPKRAVRLEAVKCRQACVSYMEFIYPQLPRVHIAIQSRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCA1</accession>
    <name>A0AAF0XCA1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0624819</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCA1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000001">
        <property type="entry name" value="Lipid phosphate phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF203">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="98"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH05403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="318" mass="35785" checksum="793374DDABB75782" modified="2024-05-29" version="1">MMQTQSGSHTVKSHGYTVARTHMHDWLILILLVVIDVILNIIHPFYRYVGKDMMSDLKFPLKSNTVPIWAVPIYAVALPMVIFLIFYLRRRDVYDLHHAILGLLFSVLVTAVITDAIKDAVGRPRPDFFWRCFPDGKDVYDQWGNVICHGNRSTVREGHKSFPSGHTAWSFAGMGFLSLYLSGKVKAFDRGGHVAKLCIVFFPLLIASLVGISRVDDYWHHWQDVFAGGLIGLTIATFCYLQFFPPPYHSQGWGPYAYFRMLEESLVNGRVPDAATTCNAQLASTQTVPQQHDRNFSGLASKGTSTQQTVEDLEAGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCK7</accession>
    <name>A0AAF0XCK7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0624987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCK7"/>
    <dbReference type="KEGG" id="dcr:108227154"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF72">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH05568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="488" mass="53692" checksum="D61B41CF4870AC32" modified="2024-05-29" version="1">MKAELIIIPAPGAGHLLSMVELAKLIVSRDARIHVSILIIRLPFESGVDGFNKDASENVAFLDVPALDDIALAEVKSLPRISFLDSFIAKHGSAVRDVVSAILKRSECSKLCGFVIDMFCTSMIDVANEFNVPAYAFYTSGAAFLTLKFYVQALKDVESKDIYKYKELDVERLAVSGFLNPVPVEVLPSVMLTKDGCPVVIAAARRLRETKAILINTVWELESHAIKSLVDDAKTPVIYHVGPVINFKSGEEPSNGRTSGEDVVSWLDRQPPSSVVYLCFGSSGSFDGEQIGEIARALELSGQRYLWSLRRPSQGKDQVVLLQDYDDYNEVLPQGFLERTSGKGKIIGWAPQLSILSHPSVGGFVSHCGWNSILESIWCGVPIATWPMYAEQQVNAFQLVVDLGLAAEIKMDYRKDRVADMESTGVVSAEEIERGIRRVMDGESEMRKKMKELKDACRKATQPGGSSYTSLGEFIQDVTNSIREGALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XG83</accession>
    <name>A0AAF0XG83_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XG83"/>
    <dbReference type="KEGG" id="dcr:108225317"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34947">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34947:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28514" checksum="8566960B6A767261" modified="2024-05-29" version="1">MVHNNKPINSSKRRIQILLSLSVLAFISSQYSSLIFTYFSSFKLFSSTTTDRHFIFLLCNGLMLLVIKTSGLFSSPAPPGTEFYRDSHVIRVQAQPSHHRSADLRENIYVKKNVISHQPVEFSKKNNVESQIDDLAETEEETDIKSVTEPEQVERRTRVMDEGCEFVTSTTDYDEKEQVSELNSVQEHEEQAEEEENEELELVNTEEEEEQETLLWSTEELNKKCDDFIRKMKYGIRYEPQLVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XGX6</accession>
    <name>A0AAF0XGX6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0625649</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XGX6"/>
    <dbReference type="KEGG" id="dcr:108192935"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000198">
        <property type="entry name" value="receptor protein kinase TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000226">
        <property type="entry name" value="receptor protein kinase TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000190">
        <property type="entry name" value="Receptor-like kinase TMK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052422">
        <property type="entry name" value="Auxin_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986">
        <property type="entry name" value="OSJNBA0070M12.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986:SF13">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE TMK1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042053054" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="606"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="451"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="634"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH06226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="952" mass="104123" checksum="64ACF62D10D3E7BC" modified="2024-05-29" version="1">MEADQIKLTVALCMCLVSLVISTTDPNDLAILKQFSKGLSNAEVLKWPNNDDPCGPPSWPHIFCTGNRVSQIQVQGLNLKGPLPQNFNQLSMLTNVGLQKNNFTGNLPSFSGLSRLRYAYLDFNNFDTIPSDFFRGLDGLEVLALDNNPLLNKTAGWLLPVDLQNSAQLANLSLMDCNLAGVLPDFLGQMSSLEVLKLSFNRLSGGIPVTFQGSVVRILWLNEQSGGASHGMSGTIDVVASMSSLTSLWLHGNHFSGKIPKNIGDLASLQELNLNSNDFVGLIPDTLATIHFKSLDLNNNHFMGPIPRFKGVNASYRTNSFCQNDPGLPCAPEVMALLEFLDGVNYPPRLVSSWSGNNPCDGPWLGLICNANREVTMINLPKFNLSGNLSPSIANLHSVSEILLSSNNLTGRIPASWVNLKSLKLLDVSGNNLSPPIPRFDSSVKLNLNGNPLLNSNKSSATAPPDNGPSPEESMSLHNNSTSESAGLKTHTKPSKKKRPKLVMFVVPVATFGILIFMAFPLSIYLCKKRRNSIENPTSLLVHPKDLSDSDNLVKVVVESNNQGSASTLTGSSLGSRYSSGMNSHITDTGNMIVSVQVLRKVTNNFAPENELGRGGFGVVYKGEFDDGTKIAVKRMEAGVTSGKALDEFEAEIAVLSKVRHRHLVSLLGYSIEGFERILVYEYMPQGALSRHLFHWKNLGLEPLSWKKRLNIALDVARGLEYLHTLAHRSFIHRDLKSSNILLGDDFKAKISDFGLVKLAPDGEKSVVTRLAGTFGYLAPEYAVTGKVTTKADVFSFGVVLMELLTGLMALDEERPEESQYLAAWFWNIKSSTEKLKAAIDPALDMKEEIFESICTIAELAGHCTAREPNQRPEMGHAVNVLSPLVEKWKPLKDEIEEYCGIDYSLPLNQMVKGWQEAEGNDLSFVDLEDSRGSIPSRPAGFADSFTSTDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIK7</accession>
    <name>A0AAF0XIK7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0728192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIK7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040389">
        <property type="entry name" value="SMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142:SF28">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH08745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10749" checksum="0ED770DF4F065DE5" modified="2024-05-29" version="1">MSTTNDADGLVENLTLENINSTNPEVLNNGCSTPKGKKFRIPRINKCPPAPMKRRSSTTTMVSSVKRSPISFFSPPDLELFFFFASDHPLCVNDNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIL0</accession>
    <name>A0AAF0XIL0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0727130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIL0"/>
    <dbReference type="KEGG" id="dcr:108193917"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000037">
        <property type="entry name" value="acyl-acyl carrier protein thioesterase ATL3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH07697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23866" checksum="07FC9201BD4D6935" modified="2024-05-29" version="1">MSQALKIPTWPTQVTLSHTRATTLSPALYRPPTNFHRPSQLRLPRVRPVPAPVRSNHGVVAAFDVKGGKGMGDFHEIELKVRDYELDQYGVVNNAVYASYCQHGRHELLERIGVNADEVAQKGDALALTELSLKFLAPLRSGDRFVVKVRVPDSSAARLYFEHLIFKLPDQEPILEARATAVWLDKNYRPVRIPPEVRSKLVQFIRHSESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XM64</accession>
    <name>A0AAF0XM64_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0730122</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XM64"/>
    <dbReference type="KEGG" id="dcr:108195235"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF22">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH10653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="295" mass="33119" checksum="E0D7DB4713E21066" modified="2024-05-29" version="1">MALLSAHWTLVFGLLGNVVSFGVFLAPIPTFYKVYKKKSTEGFQSVPYVVGLFSAMLWIYYALLKSNTLLLITINSAGCVFQTVYICIFLIYAPRKARMQTLKLLVSMNVVGFGLIVILTQFVAKGAANRVVIVGWICLVFSLCVFVAPLCVVRQVIRTKSVEYMPFLLSFFLTLSAVMWFFYGLLLKDCNIAVPNVLGFTFGILQMILYMVYRNSKKDSIEAKKLPELFEDDSVIIIDDQKLPELKAKIDDVMRLSAMVCSEMKPVGRNSNPNELDMIEIQVVVPKKQATPITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPL7</accession>
    <name>A0AAF0XPL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0730287</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPL7"/>
    <dbReference type="KEGG" id="dcr:108193571"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000298">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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            <dbReference type="EMBL" id="WOH11582.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTL0"/>
    <dbReference type="KEGG" id="dcr:108199428"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000173">
        <property type="entry name" value="Probable serine/threonine-protein kinase At1g01540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF21">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="179"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="104"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH13112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="499" mass="55535" checksum="A920B73336B03E0F" modified="2024-05-29" version="1">MGIVNDLTRETPIFHIPVWGLVLIFVLVPVILLVLPICISRRKSRVGSEKLPVSQNKVMSSEIRDIRVDHNSGNQYAYEGDPKILMDKYSGRDPDKYLMHLDRKKNADNSSGSGSFITTENGDYGSGSGEKGTSGKNKAYTPSRLLNNSSPLSGLSDFSQLSWGHWFTLRDLQVATNRFATDNVIGEGGYGVVYHGHLANGSSVAVKKLFNNQGQAEKDFKVEVEAIGNVRHKNLVRLLGYCIEGTQRMLVYEFVSNGTLDQWLHGDMSHQGYLTWEARMKVVLGTAKALAYLHEAIEPKIVHRDIKSGNILLDDDFNAKISDFGLAKLLGAGESHITTRVMGTYGYVAPEYANSGLLNEKSDVYSFGVVLLEAITGRDPVDHGRPADEVNMVDWLKSMVANRRSEEVADPTMETKPPRTALKRALLVALRCVDPDSETRPSMSKVVRMLESDEYPLPAEGRRRHKNKVAATETDCRTSNTAKYKVYNLRPENRDNQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUF7</accession>
    <name>A0AAF0XUF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0934020</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUF7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH14501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="138" mass="16195" checksum="57A5239372080B99" modified="2024-05-29" version="1">MEGQVGIVTQLLETFLSALLRNKASIAIFLMFFILFRLLIKKFLHHHYHIPHFTFNQNPKSEPLIYCAVCLHDVANGEMCRKLPQCEHCFHVDCIDVWLRCHLSCPLCRSRVDYYLSKDKNYKSGFSSYILPVIRKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUW0</accession>
    <name>A0AAF0XUW0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933929</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWZ4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000317">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF592">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="617"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="677"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOH14379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="991" mass="109762" checksum="D72A46978DEB7649" modified="2024-05-29" version="1">MTFNFLQFNILFFFVLGTSTTFVYSFSNNVTDQQALLSFRYSITDDPSGVLNSWNNSIHFCHWAGVTCSRRRQRVTALNLSSLDLGGTLSPHTGNLSLLRSISLHRNRFHGLIPNEIGRLVRLQYLNLGNNSFQGGFPANLRHCRDIRYINMDGNDLQGKLPTFSSWTKLAATNYLTGEIPVELSHHAKLEILDLQVNSLSGMVPLPLYNILSLYILGLNNNVLKGSFPSYLGLTLPNLQGLYASQNRFSGPLPPSIVNASNLVLFDVQNNSFTGPIPNNLGTLPNLQILDLGFNPLGDNILPNDWKFFNSLVNCTQLNELDLHESSLRGELPNSIANLSTTMERLYMQDNSIYGSIAPEIEKLVNLIYLDFRDNFLTGSIPQSIGKLSKLGGLYLSGNNISGVIPTSISNITQLFALAVQNNQLEGSIPTQLFNISTLGEVSLAHNRLRGAIPDEIVFISTCVYLDLSRNLFSGPLTPNIGSLKHLSVLDLSYNKLIGDVPATLDGCLMLEELYMAGNLLQGKIPSSFKILKNLAFLDLSNNNISGSIPNYFGGFRQIIFLNLSHNKLEGEVPEEGLFSNVSAFSIIGNLELCGGIQVLHLHACPRKVSRNKKKAFALRIIVIAVLVPLGLLFACIALIFYRRRNSKKLNEPVPVLKDDRYPRFSYQDLLRATNEFSPDNLIGEGKYGSVYEGTLESIEHIVAIKVLNVEVHGASKNFLAECETLRSVRHRNLIKIITACSSTDFKGNDFRALVFEFMINGSLDNWLHPSLPNQGNIRNLSLLQRLNISIDVALGVDYLHHHSHASIIHSDIKPSNILLDEGFVARIGDFGLARFGFSTTTSDINRARMSSTGVRGTVGYVPPEYGMGVEISAKGDVYSYGILLLEMFSGKRPTCSSALLENCSNLHDYVKKALPQSVMNITDPRIKTEEEEDGSEKKHSHRKANVAACLTSVFEVGILCSVEIPRDRIDISVAIKQLHVARDKLLQCKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XXP2</accession>
    <name>A0AAF0XXP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0934438</name>
        <name evidence="2" type="ORF">DCAR_0934454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XXP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052343">
        <property type="entry name" value="Retrotransposon-Effector_Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890">
        <property type="entry name" value="NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46890:SF48">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH14909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13327" checksum="0072A1E9913E292D" modified="2024-05-29" version="1">MPGIIDKAQSAFVPGRQISDNILLAQELFRGYGRETGLPKCALKLDLNKGLRQGDPISPYLFTLAMNVFSSILNQVPTGFQYHWKCKELKLTHLFFADDTQQRLFWTTKAPSWHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XXW6</accession>
    <name>A0AAF0XXW6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0934834</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XXW6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="16">
        <location>
            <begin position="298"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="668"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="621"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH15297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="745" mass="84586" checksum="4CDAF4596BE7A714" modified="2024-05-29" version="1">MASRALLRRKKDIFYSLRGPTCLLRGSSRVVDSLDVDVGTVHSFRDSKNTNYRSSSSLAKEEVLIFSAKGFLRRIASTNQSLYPGITEIGFRLPLRGRSLAESIVHFSSAGTSKLEPDSGNDEKADTLKKEASPEECDEAVEGLTTAKAKAKAKQLQESKKDTRSILEKVWAFFLGIGPALRAVASMSREDWVKKLRHWKDEFKSALQHYWLGTKLLWADVTISLRLLQKLANGKNLSRRERQQLTRTTADIFRLVPFAVFIIVPFMEFLLPVALKLFPNMLPSTFQDKMKEQEALKKKLNARIEYAKFLQDTVKEMAKEIQNSRSGEVKKTAEDLDEFMNNVRRGTRVSNDEILGFAKLFNDELTLDNISRPRLVNMCKYMGISPYGTDAYLRYMLRKSLQKIKSDDKMIQAEGLDSLSEEELRQACRDRGLLGLRSVDEMREQMRDWLDLSLNHSVPSSLLILSRAFTVSGKLKPEEAVQATLSSLPDEVVDTVGITSLPSEDSVSERRRKLEFLEMQEEMIKEEEEKEEEQAKKKESLEKQKDVALEEMVVPTAQEADKLAKAKTLDKQEQLSELSRALAVLASASSVSREREEFLRLVNKEIELYNRMVEKEGTETEEAAKKAYRAAREESNDTEEKTVDAKVSSALINRVDAMLQKLEKEIDDVDAKIGDRWRLLDRDYDGIVSPEELASAAMYLKDTLDKEGIQELISNLSKDIRVEDILKLASQSKEEDETGNEAGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYI8</accession>
    <name>A0AAF0XYI8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0936136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108201737"/>
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    </dbReference>
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        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040389">
        <property type="entry name" value="SMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142:SF40">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="107" mass="12407" checksum="2428B53E87B9F7D8" modified="2024-05-29" version="1">MGCSKKDEGVIVESEAKKRVVLTEITSYTPLKPIFTRQEFKKHEEEEEENSRSTTPKSEESRVSKFHCPPAPRKRKTKPRCYYGGVREFFAAPPDFESLFIRHAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIB9</accession>
    <name>A0AAF1AIB9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0100614</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIB9"/>
    <dbReference type="KEGG" id="dcr:108203902"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17939">
        <property type="entry name" value="DEADc_EIF4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="59"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="293"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG81467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="413" mass="46614" checksum="D560589DDA26AEA6" modified="2024-05-29" version="1">MAGAAPEGSQFDARQFDAKMTELLGAEGEDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQQLDYSVVECQGLVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILSSGVHVVVGTPGRVFDMLRRQSLRSDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKVQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTKDDDRMLVDIQKFYNVVVEELPANVADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIF1</accession>
    <name>A0AAF1AIF1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ripening-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0100385</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIF1"/>
    <dbReference type="OMA" id="HNNITIT"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF58">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042074256" description="Ripening-related protein 1" evidence="5">
        <location>
            <begin position="25"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG81240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20162" checksum="AE5348F7F4034D55" modified="2024-05-29" version="1">MKHIYFCLIQFLVFYNLSFISVEAQECQSSGTIAGTSPPNGQCNTAYGAACCEQDKIYDIFTCSPPLSGATTATLTLNSFEDNGDGGAPSECDQKYHSNETPVVALSTGWFNNMSRCNKNIVIYGNGMSVTAVVVDECDSTTGCDQDHAYQPPCDDNIVDASEAVWTALGVPRSEWGSMQISWSDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANF3</accession>
    <name>A0AAF1ANF3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0208394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANF3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35631">
        <property type="entry name" value="OS08G0114150 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35631:SF6">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG89158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="70" mass="8189" checksum="6A57AD2B0B784692" modified="2024-05-29" version="1">MKSIPFSAFWLRSSPVTIACYWGLRESPKRCTTALAWLTPPNLIKSSKFLIPMKFKFYRNIMSLSCNLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AR26</accession>
    <name>A0AAF1AR26_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311588</name>
        <name evidence="3" type="ORF">DCAR_0311589</name>
        <name evidence="4" type="ORF">DCAR_0311590</name>
        <name evidence="5" type="ORF">DCAR_0311591</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AR26"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG92326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG92327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="17847" checksum="0DE0C21E16067512" modified="2024-05-29" version="1">MVPKFAFTTWLIVQERLLTKDRMINFRMRTTSSCVLCGVADESHAHLFCHCTYIRSIFASWNHGITSVWDDLKSGRIFTGQISSIEKEISFLFISSVFYSVWRERNLRVHEGGTHNSWASLLVNIKRDVKGKLASSARFRKCVHRDTTLVLNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARV0</accession>
    <name>A0AAF1ARV0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0309861</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARV0"/>
    <dbReference type="KEGG" id="dcr:108215346"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009805">
        <property type="term" value="P:coumarin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000357">
        <property type="entry name" value="Caffeic acid 3-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000061">
        <property type="entry name" value="Caffeic acid O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="141"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="364" mass="40226" checksum="1C9ACABDB082F182" modified="2024-05-29" version="1">MTLTTEMNPKVQTEGEEEGAYMFAMQLVGASFLPMVLMSAIELDLLEAISKAGPGAYVSPSELADQLPSSQQGTPVMLDRILRFLASYSVLECRIRESTDGHIERLYGLAPVCKFFVKSSDGVSMAPYLLMNHDKIFMETWYHLKDAVLDGGIAFEKAHGMNVFEYNRKDPRFSQVFNQAMSSHSTLTMKKILQTYNGFEGLTTLVDVGGGTGATLSMIISSYPAIKGINFDLPNVVKDAPPYPGVEHVGGDMFVSVPRGDAIFMKYVCHNWSDERCLKLLKNCYQALTKKGKVIIVESISQEVPDSDIATKHVYDIDVAMLTRTPSGKERTENEFAALIKDAGFTGYSKASCGYNISVMELYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASZ2</accession>
    <name>A0AAF1ASZ2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0310524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the translin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASZ2"/>
    <dbReference type="KEGG" id="dcr:108210875"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14819">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.190:FF:000001">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.190">
        <property type="entry name" value="Translin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.200">
        <property type="entry name" value="Translin, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033956">
        <property type="entry name" value="Translin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016069">
        <property type="entry name" value="Translin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016068">
        <property type="entry name" value="Translin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33939" checksum="4AAA3E6C2C865649" modified="2024-05-29" version="1">MRLAFRNASGAAKLSINLSEAGCSSFLNPNPSFINTCPIHHHISPRNAVVSNPSRCHTLSGAETHAPPFSALMDKQFEAFRQHLEESGSLRDRFRAVATQIEATNRNINSFLLLIHQSRPMPEVLEKVNSEIDVLKRLFNQLAEIARECPGQYYRYHGNWKSETQAVVSLLAFLHWLETGTLLGHAEAEQKLGLDKSEFRIDVEDYLIGICFMANDLPRYVVNRVTAGDYDCPRKVMKFLTDLHAAFRMLNLRNDFLRKKFDGMKYDQRKVEEVYYDVKIRGLAVDGDTMVEGTEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATV2</accession>
    <name>A0AAF1ATV2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATV2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000004">
        <property type="entry name" value="Ribosome production factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="76"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="249" mass="28872" checksum="84BED8834E1C209C" modified="2024-05-29" version="1">MIKNKDKRSAVHAKLKEQKKLEKRKKAKLLPPRPVPRTIENTREAHETICKPDEEELFAANDVDEFSSLLKQEHVPKILITTCRFNSTRGPEFISDLLSVIPNAHYYKRGTYDIKKIVEYAKKDFTSLIVVHTNRREPDALLVIGLPDGPTAHFKLTKLVLRKNIENHGNPTSHKPELVLNNFTTQLGHRIGRLIQSLFPQDPNFRGPRVVTFHNQRDFIFFRHHRQSGEFVTEGEEIFPEINNSRNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUL3</accession>
    <name>A0AAF1AUL3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Uncoupling protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0415298</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUL3"/>
    <dbReference type="KEGG" id="dcr:108219076"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000019">
        <property type="entry name" value="Mitochondrial uncoupling protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="215"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG95968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="32448" checksum="20B487C243792638" modified="2024-05-29" version="1">MVGGGSSNSDISLPGIFASSAFAACFAEICTIPLDTAKVRLQIQKKAVAGDGVALPKYRGMLGTVGTIAKEEGLSALWKGIVPGLHRQCLYGGLRIGLYEPVKNLYVGENFVGDVPLMKKILAGLTTGALAIAVANPTDLVKVRLQAEGKLAPGIPRRYNGALNAYSTIVRQEGVRALWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVFTHLLSGLGAGFVAVCVGSPVDVVKSRMMGDSTYKNTLDCFVKTLKNDGPFAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVKSIESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW50</accession>
    <name>A0AAF1AW50_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0414672</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW50"/>
    <dbReference type="KEGG" id="dcr:108218848"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14319">
        <property type="entry name" value="UBA_NBR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000199">
        <property type="entry name" value="next to BRCA1 gene 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000085">
        <property type="entry name" value="protein NBR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056893">
        <property type="entry name" value="UBA_Nbr1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24932">
        <property type="entry name" value="UBA_NBR1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PB1" evidence="14">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="13">
        <location>
            <begin position="371"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="195"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG95357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="787" mass="86415" checksum="E0816E789B54959D" modified="2024-05-29" version="1">MEPSLVIKVQYGETLRRFSSHVVDGKLDIDMNGLIEKVCSLFNFSSGSELDFTYVDEDGDVVKLADEEDLHDILRQSLNPLRITVKLNTEWSVSSRPNGRTNGNSTPLRSPQLQFPLQVENAAVAEVLNSLPSTIRETTAKFSTELGLDTNSPAGYLAVLDSLAKMGLSYLKDVSVTGAKSGSTVISSTDATVGKEKNGSISVSTTPATEKKTSSISFRTTPATKDQTSSVPVQTTLVPELKGLGTDEGNAAEGFVMPALDNTNAKDESSILPKITATNLLNEMKGPSDFVSQENKVGKNGRSGPKMKPVKKSFESHHGWKSVMSPKPLTGIDSPSSGLPVPPYLHRHQLPYRVAPVRSYNKSDVMASVFHTGIQCDGCGVHPISGPRFKSKVKEDYDLCIFCFNHMGSEAEYIRMDLPMNYQGRDPLNFPFPHRLGRPPTLPADHAKLDSCFILDVNILDGTILTPLTHFTKIWRMRNNGAVAWPHGTKLLCIGGDKLSKTHSCDLKIPAEGFPVEKEIDVEVDFAAPENPGRYISYWRMASPSGQQFGQRVWVLIQVESSLKAPNSKNRDFNLNFPPVGSGIMGLQNENSSVDLKTGDDLTEIDASTKDQHAPVGRKISEDLIVIKKPTMDKGIDLNFWNNGTLLVARPKPDSAAPGASSTVTYPVVDQSKVEPVVISRQQKLDLDIPSSMIDANQGTAEVEQTLLKELENMGFTEIDLNKEILRKNKYHLEETLNELCDTEWDPILAELQEMGFSNNEMNKKLLKKNNGSIKRVVMDLIAGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AY99</accession>
    <name>A0AAF1AY99_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AY99"/>
    <dbReference type="KEGG" id="dcr:108221257"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070:SF120">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="30336" checksum="AAD31E6ED0A521E3" modified="2024-05-29" version="1">MTHSRSISLPSRPHPSAADVEEQLCRLRSSDETSTSTLCNKLSGLKYLYECVDDYLQLPMRHSSDSEEALGASIRLLDLCNLTKEAFSQMRASVQDLESSLRRREADVSSKIGSYLICMKKVNKMVSKCLASFKKSQNKKCHETSATVSLLREVEEVSITMFESLFSSVFPAKETSKQNRWSLVFKSKQSKRVHEVSEGKVEEMQMDIEAIYKQKINMESDFSRIQGAQKCLMALDKNMQQCEEDLECLYRSLIKTRVSILNVLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYG9</accession>
    <name>A0AAF1AYG9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0416616</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="6">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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    </reference>
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        <text evidence="5">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
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    </dbReference>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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            <dbReference type="EMBL" id="WOH04549.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="entry name" value="F3I6.5 PROTEIN"/>
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        <location>
            <begin position="59"/>
            <end position="89"/>
        </location>
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    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="176"/>
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    </feature>
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        <location>
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        <location>
            <begin position="311"/>
            <end position="340"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH16508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="44846" checksum="CAB03D2DCB7E49A8" modified="2024-05-29" version="1">MGIHPFPLSHTYDQESTDLTSDSSMNSSISSQPSLPLVPSLNKLSQEQSNIHHHCISTLKGHSSYVFSLTLSGKHLYSGSSDSQLRVWSRAPSDHQNSDENIVSACKSAVKCIVVLDDKIFSAHQDHKIRVWKIGSDKSKQAYKCVSTLPTLNDRVTRLFFAKNYVEVRRHKKCTWVHHVDTVSALALSSRDSSFLYSASWDRTFKIWRTSDFKCIESVWNAHDDAINAIVSSGDGFIYTGSADRKIKVWGKKEGEKRHTLIDTLEEHKSAVNALALSSNGSVLYSGACDRSIIVWERAFGSRHMAVAGALRGHTKAILCLAVVSDLVCSGSADKTVRVWRRGNDNSYSCLAVLEGHRGPVKCLTAAIDHSNSDNNSSPIGTPYLIYSGSLDCDVKVWQIWVPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WSE3</accession>
    <name>A0A161WSE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_010339</name>
        <name evidence="5" type="ORF">DCAR_0311772</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fantastic four family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WSE3"/>
    <dbReference type="STRING" id="79200.A0A161WSE3"/>
    <dbReference type="Gramene" id="KZN01585">
        <property type="protein sequence ID" value="KZN01585"/>
        <property type="gene ID" value="DCAR_010339"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215308"/>
    <dbReference type="OMA" id="NEECGPW"/>
    <dbReference type="OrthoDB" id="676808at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021410">
        <property type="entry name" value="FAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046431">
        <property type="entry name" value="FAF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155">
        <property type="entry name" value="FANTASTIC FOUR-LIKE PROTEIN (DUF3049)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155:SF9">
        <property type="entry name" value="FANTASTIC FOUR-LIKE PROTEIN (DUF3049)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11250">
        <property type="entry name" value="FAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAF" evidence="3">
        <location>
            <begin position="160"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN01585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG92503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="265" mass="29855" checksum="79D317BDBB776D8F" modified="2016-07-06" version="1">MAACESLQHIFETPSPDSPKDLLQSFSTWKHLPPLEVSSLTEVFGELHFEENKEPVSPSLPHSALSSPFSIDSNPRSEEKNEGNYTPPTRKHYKNSDSFSSMNSESLSICTEGLGFESCDNTEDFVDNSGCKHRKARSSITRHFSNEYLSGKRSRITGGEFPPPISCIGRTGKPWVCFKSYRQNGRFVLKEVKTPIQEFLHACRQDGRLKLRLIQSDDEITEEEEEDVSEGECQDEDENSRIHNFGEGEVSKGEKIVEDEGSVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WV21</accession>
    <name>A0A161WV21_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_011319</name>
        <name evidence="3" type="ORF">DCAR_0312804</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WV21"/>
    <dbReference type="STRING" id="79200.A0A161WV21"/>
    <dbReference type="Gramene" id="KZN02565">
        <property type="protein sequence ID" value="KZN02565"/>
        <property type="gene ID" value="DCAR_011319"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210735"/>
    <dbReference type="OMA" id="SNPFPWM"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN02565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="329" mass="37621" checksum="DD9A8DBADF12CD41" modified="2016-07-06" version="1">MPALPEEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDNVHWEALTSDEKHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLESYIKDSEEKARLFRAIETIPCVEKKAKWAMRWIDGGESFAERIIAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACMLYDLLRMKPSEERVKGIVADAVEIEREFVCDALPCALVGMNGDLMSQYIKFVADRLLGALGYGKMYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSINGNADSHVFKLDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XDD7</accession>
    <name>A0A161XDD7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0624270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XDD7"/>
    <dbReference type="Gramene" id="KZM90723">
        <property type="protein sequence ID" value="KZM90723"/>
        <property type="gene ID" value="DCAR_021912"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225276"/>
    <dbReference type="OMA" id="QDKIKWR"/>
    <dbReference type="OrthoDB" id="685795at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040262">
        <property type="entry name" value="At4g38062-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45287">
        <property type="entry name" value="OS03G0691500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45287:SF3">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="920" mass="106748" checksum="D007819251370201" modified="2016-07-06" version="1">MNGVCDELDEVRAELEKLKEECRSKTKLIDSLRTAHNEQKSKLQGAMAQIEELAQELRVKTEEIPELKKLYEELELKVHEKESLLRHLNSANEKLRADYGARFQKLEAENKELAAALDEATERIQDLELKDRSRNEEVEGLKRLISNKQKKFFESDQNAIEDKEGKQKEVVILRLEDENRHVQEQLKWKNEQFAHLEDAHRKLQEQFRSSTVDWKRERSALIEEISSLQSSLDSQTRISETLQTRLKMCNQALAHEESRRKILEVEVSELKSHFETAFSSSHETKTNSDHLTFKRDEEISNLRNLIGTKDINSKEMEHRIAQLEQEKQGLKDSLKESEINNAELTLSLKKLEYKIQDLEQLHENTLINLQKKEDDHSYQIEKLTEVVEGYKSKLKGKNKKLQELQNDLDGYCCLLEAQSEEISTVMMIMKSEYSAAYLKLINETSEKDVRNKESEEKVLLLTTQLEMKDSAFHSLNQKLEKKNEEATSLVERVKSYDCMEQQYIFMEEELQRHKKMLAESSENQNCLKKQVLNVESTLKMHHHESNSALEKANAELARRISEVSNVEAELQKWRSHAENLDLCLRESQETSCAENICLKSAVKKQDEEICSLQQQIASLESRHAEKIDAILKALEAEKEHCIRTAEDDRSTIEKLQLEILSLTQNFSAREMDIVHAQEDAVKALEQEKNSLLLIIRDKNQLVAHLSEQAKLTEQHFTSTFVCLVDKQIEVDFLSESLDKTRNLKKAEIEEKEKIGTALRMEIKNLQEKLVDQEEYLLQMEQKTEQLEALLQTNNSEIIKLNYQSSFEQKHLESLVKELELQKGALLEDITKLSAHRDDLLTQLDRFLEQIDVFSGKDLELVAKLENISHTFEEEKNKELESLADSGLNNSPSKIVKQLHISEDGTGGTIEQRFPLSELNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y0J9</accession>
    <name>A0A161Y0J9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Miraculin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_011411</name>
        <name evidence="4" type="ORF">DCAR_0312911</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I3 (leguminous Kunitz-type inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y0J9"/>
    <dbReference type="STRING" id="79200.A0A161Y0J9"/>
    <dbReference type="Gramene" id="KZN02657">
        <property type="protein sequence ID" value="KZN02657"/>
        <property type="gene ID" value="DCAR_011411"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211554"/>
    <dbReference type="OMA" id="CLAFICK"/>
    <dbReference type="OrthoDB" id="1872570at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23375">
        <property type="entry name" value="beta-trefoil_STI_VvMLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007829405" description="Miraculin" evidence="2">
        <location>
            <begin position="20"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN02657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22846" checksum="89152F039433FFDA" modified="2016-07-06" version="1">MKPYIFLPFFLLLVPCALAASAPNPVIDTFGRYLRQGVSYYIVPVPPEDPTVSSRGGGLNLAATRNKTCPLDVVQEMDENNKGLPFSFIPINPKKGVIRESTDLNIKYMGDTACNESMVWMLNQLGDPNGPYFVTTGGVEGNPGAQTIGNWFKIEVFIDDYKIMYCPTVCKYCKVICRDVGIVVVNGRRYLGLTDQPRRVMFKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y732</accession>
    <name>A0A161Y732_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y732"/>
    <dbReference type="Gramene" id="KZN05247">
        <property type="protein sequence ID" value="KZN05247"/>
        <property type="gene ID" value="DCAR_006084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="72" mass="7769" checksum="DBF300C8C15E9F03" modified="2016-07-06" version="1">MSSRDMTNPSQGSVRVGRVEPGCENRAAAATVQLRSSEKYKVQAEETSDCWTNAGMVGGCDQSDNEGEKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZU93</accession>
    <name>A0A161ZU93_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_022325</name>
        <name evidence="4" type="ORF">DCAR_0623859</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZU93"/>
    <dbReference type="STRING" id="79200.A0A161ZU93"/>
    <dbReference type="Gramene" id="KZM90310">
        <property type="protein sequence ID" value="KZM90310"/>
        <property type="gene ID" value="DCAR_022325"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225990"/>
    <dbReference type="OMA" id="SEIRTMC"/>
    <dbReference type="OrthoDB" id="25592at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF18">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM90310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH04450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="326" mass="36035" checksum="3AF5644A670EC53D" modified="2016-07-06" version="1">MASLWVKRKFLGNGSYGKVFLAQSTALLAYYKSPALVPFVALKSCLASRSSSLRLERDVFRELGGCDRIVKCFGSGVSVENGVEYFDLVLEYAPGGSLEQLVRFRGGRVPEMEASCYTRMLLKGLSHVHGKGWVYCDLKPANILVFPSKHGGGVFKCSLKLADFGSAKRGGEISCGFVDPCSGRKNRGTLLYSSPESVEFGEHEAPMDVWALGCIVFEMLIGDNMWSNYRSFNARVLGDLIAGYQDSGLIFCDDLSANAEDFLRKCLTRDVEDRWTADQLLEHPFITESCKMLPLSESGQTPKIMGYQRIPFSSPFVVNNPSVFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZVV0</accession>
    <name>A0A161ZVV0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_021565</name>
        <name evidence="5" type="ORF">DCAR_0624677</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZVV0"/>
    <dbReference type="STRING" id="79200.A0A161ZVV0"/>
    <dbReference type="Gramene" id="KZM91070">
        <property type="protein sequence ID" value="KZM91070"/>
        <property type="gene ID" value="DCAR_021565"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227754"/>
    <dbReference type="OMA" id="TLWENNW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF17">
        <property type="entry name" value="PROTEIN DELETION OF SUV3 SUPPRESSOR 1(I)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01385">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM91070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="9008" checksum="208CE92A77E0D824" modified="2016-07-06" version="1">MADTKAVEKAEAVAKIDLFEDDDEFEEFEIGQEFDEKKEVGSDVTQQWEDDWDDDDVSDDFSLQLRRELVNNSTEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZWK7</accession>
    <name>A0A161ZWK7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_021215</name>
        <name evidence="3" type="ORF">DCAR_0625108</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZWK7"/>
    <dbReference type="Gramene" id="KZM91420">
        <property type="protein sequence ID" value="KZM91420"/>
        <property type="gene ID" value="DCAR_021215"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM91420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10856" checksum="12CFFE1465255BFD" modified="2016-07-06" version="1">MDSRSQQPMINLSGSGRKRNKENQSDASLAGIRRRGSGNALDLFLQRRAESIKRSRTIGTSSEFDKNSRATSQVPLSTTDQSAPYQCGFKHDFRLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A1Q9</accession>
    <name>A0A162A1Q9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_017055</name>
        <name evidence="8" type="ORF">DCAR_0519510</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A1Q9"/>
    <dbReference type="STRING" id="79200.A0A162A1Q9"/>
    <dbReference type="Gramene" id="KZM93810">
        <property type="protein sequence ID" value="KZM93810"/>
        <property type="gene ID" value="DCAR_017055"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220438"/>
    <dbReference type="OMA" id="GMMDCLL"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="65"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM93810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH00152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="198" mass="22673" checksum="25A377EF98E4FC52" modified="2016-07-06" version="1">MGRAKKGPKFAVVKKMITSKAIKTYKENVLNPNKKDLQSEKLPRNVPSTSSALFFKYNTALGPPYRVLVDTNFINFSIQNKLDLEKGMMDCLYAKCTPCITDCVMAELEKLGQKYRVALRIAKDPRFERLPCTHKGTYADDCLVERVTQHKCYMVATCDRDLKRRIRKIPGVPIMYITKHKYSIERLPEATMGGAPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ARX1</accession>
    <name>A0A162ARX1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0206548</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ARX1"/>
    <dbReference type="Gramene" id="KZN04983">
        <property type="protein sequence ID" value="KZN04983"/>
        <property type="gene ID" value="DCAR_005820"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208386"/>
    <dbReference type="OMA" id="MAIMSQQ"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03564">
        <property type="entry name" value="ANTH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000005">
        <property type="entry name" value="Clathrin assembly protein AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000003">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG87324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="576" mass="65462" checksum="A99997DF66A6A291" modified="2016-07-06" version="1">MGSFDSFRKAVGALKDTTTVQLVKLNSDFKDLDIAIVKATNHVECPPKERHIRKIFWATSAVRPRADVAYCIHALARRLSKTRNWIVAIKALIVIHRTLREGDPTFREELLHYSHRGQILQIYNFKDDSSPLAWDCSAWVRTYAQFLEERLECFRTLKYDIEAERLTKSIPGTSKAHSRTRLLNGEELLEQLPALQQLLHRLVNCQPEGAAYHNYLVQYALALVLKESFKIYCAVNDGIINLVDQFFEMNKYDAVKALDIYKRAAQQAESLAEFYDISKGLHLARNFQFPTLKQPPASFLATMEEYINEAPRNSSESIKRLEYAPEPEQPEEPEPEEEEVEEVEEKEEETQEEPETKEEDQVPPPHEEMGDLLGLNEEINPRAAELEESNALALAIIQPGSEQQSTSNALSEIGKTSGWELALVTTPSNTSNNNQPQNYNLGGGFDKLLLDSLYEDDSARRQIQLQQAGYNTYGYGMPSNSLNPFEQQGDPFAMSNTIAPPTNVQMAMLQQQQMMMQQQQQQNNMMMVPHQSFQNAPPYPQQQQQMQPYMGAPNPFGDPYSFSANNAPSQGHNPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AT31</accession>
    <name>A0A162AT31_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0207173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AT31"/>
    <dbReference type="Gramene" id="KZN05523">
        <property type="protein sequence ID" value="KZN05523"/>
        <property type="gene ID" value="DCAR_006360"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207565"/>
    <dbReference type="OMA" id="WLNTRER"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016138">
        <property type="term" value="P:glycoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044:SF39">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="457" mass="51673" checksum="BD0B582A7259ED10" modified="2016-07-06" version="1">MATRNTANLSVLMLPWLAHGHISPYLDLAKKLSTRNFNIFLCSTPINLESVKNKVTGKWSESIQLVELNLPPSPDLPPHYHTTNGLPPHLMVSLETAFADSSPNFLTIFESVKPDMLIYDYNQSWAADIALSNNIPAVQFLLSSAIFISLTRHMLYCDSSVTYPFPISIHKYFTEKMQARIKSSPDDAKYIDRARGASKKSRVILLRTFREIEGKYVDYISNLSQKKTIPVGSLVQESLQETGDDCTETIQFLNKKDESSVVFVSFGSEYFLTKEEIQEVAYGLELSKLNFIWVIRFPFGENTKLEEALPIGFLDQVGDRGLVVEGWAPQARILRHSSTGGFISHCGWSSIMESMMFGVPVIAMPMHIDQPFNTVVVKEVGVGQEVERDEDGRFKREEIAKVIRNVVIEKSGETVRRKAKEMREMIREKGEKEIDEVVGELVNLCKEKKDKVTSSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TSI7</accession>
    <name>A0A164TSI7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0728704</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sigma-70 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TSI7"/>
    <dbReference type="Gramene" id="KZM87901">
        <property type="protein sequence ID" value="KZM87901"/>
        <property type="gene ID" value="DCAR_025002"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196736"/>
    <dbReference type="OMA" id="CAELNGS"/>
    <dbReference type="OrthoDB" id="206108at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06171">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.601.10">
        <property type="entry name" value="RNA Polymerase Primary Sigma Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016262">
        <property type="entry name" value="RNA_pol_sigma_SigB/C/D/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF57">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000767">
        <property type="entry name" value="RNA_pol_sigma_SigB/C/D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH09248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="576" mass="64472" checksum="6C5B1DB9227BDA38" modified="2016-07-06" version="1">MSCLLPQFKCSFSIHLRPLLHQTSTQPLKDREPFYFRAQCSATVTTVLDIPSLEAHSNPVAANRPWSPSTSTAVLDLEKLHLASLGIHSDFAATNRPWTYVSDIDPALEASFGASLPTKTSLTSKEAVIAAAALEAVALAREAAKTAKDAVMMVNHINSTTSYIKALAERPQPQLRQTVDTMVVGESEVAQSECVSDVVEPTAEEAELLQAELVKSIAVRSRRQPQRKARRARAAKNTSATVVSVKSGSSSQKRRGSREIDHSDPLRYLRQTSTSSILLTATEERELSKGIQVLLRLEKIYKELSERYGGEPSFMQWALAAGVDQTTLRRQINYGTFCKDKMIMSNVRLVVSIAKKYVGTGMNFQDLVQEGCRGLVRGAEKFDASKGFKFSTYAHWWIKQSVRKFLSDQSRTIRLPFHMVDATFKVKEARKNLYNANGRKASNEEIAEATGLSMRRLSAVLLVPKAPRSLDQNIGFSLNLKPSEITADPEAETSEELLIKQSMRQDLEKVLNSLKPREKQVVICRFGLDDGRMKTLQEIGELMGVSRERIRQIELCAFRKLKNKRRTKRLHQYQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U0S8</accession>
    <name>A0A164U0S8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cx9C motif-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_025353</name>
        <name evidence="6" type="ORF">DCAR_0729105</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CMC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U0S8"/>
    <dbReference type="STRING" id="79200.A0A164U0S8"/>
    <dbReference type="Gramene" id="KZM88278">
        <property type="protein sequence ID" value="KZM88278"/>
        <property type="gene ID" value="DCAR_025353"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195197"/>
    <dbReference type="OMA" id="CNYNSTH"/>
    <dbReference type="OrthoDB" id="13601at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027179">
        <property type="entry name" value="CMC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590:SF0">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08991">
        <property type="entry name" value="CMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM88278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH09647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7139" checksum="3DE7C9422E9F64DA" modified="2016-07-06" version="1">MAQKQNKEPCKQEACNIQACLSKNNFLPQKCVRVIELLQSCCEQCKYQSTHCASVAALLKQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164V9F3</accession>
    <name>A0A164V9F3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="10">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0623535</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="10"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164V9F3"/>
    <dbReference type="Gramene" id="KZM90005">
        <property type="protein sequence ID" value="KZM90005"/>
        <property type="gene ID" value="DCAR_022630"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192911"/>
    <dbReference type="OMA" id="RISCVYK"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010227">
        <property type="term" value="P:floral organ abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010229">
        <property type="term" value="P:inflorescence development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000350">
        <property type="entry name" value="Mitogen-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000388">
        <property type="entry name" value="Mitogen-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF762">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0381">Hypersensitive response</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="66"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="321"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH04127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="357" mass="39420" checksum="3BD58D81AD76F115" modified="2016-07-06" version="1">MKSVQPPVPAAANRPRPRRRPDLTLPLPQRDTSIAVPLPLPPNSAPSSTTSASAAAAAQPINFSELDRINRIGSGSGGTVYKVAHRPTGRMFALKVIYGNHEESVRRQICREIEILRDVDNLNVVKCHDMYDHAGEIQVLLEYMDRGSLEGIHITNEAALADLTRQILSGIHYLHRKRIVHRDIKPSNLLVNSRKQVKIADFGVSRVLAQTMDPCNSSVGTIAYMSPERINTDLNQGRYDGYAGDIWSLGVSILEFYMGRFPFAVGRGGDWASLMCAICMSQPPEAPPTASRHFREFISCCLQRDPHRRWTANQLLRHPFVTQTNNGTPPNGNGGGSQVNQAHQLLPPPPRPHPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VEZ7</accession>
    <name>A0A164VEZ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_022389</name>
        <name evidence="9" type="ORF">DCAR_0623780</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VEZ7"/>
    <dbReference type="Gramene" id="KZM90246">
        <property type="protein sequence ID" value="KZM90246"/>
        <property type="gene ID" value="DCAR_022389"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31989">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
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        <location>
            <begin position="445"/>
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        </location>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM90246.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="483" mass="55937" checksum="78B846C2F5C0D864" modified="2016-07-06" version="1">MEKYGCEACIVEFAMKGFIFEPSAQEICEIYLKNKIEGKKLPCKHLLFDENMYEDHSTPWEVFKSDDPRWLINNKTKKVMYVFTELKKKGERVLRKAGCGTWTGQNRKVVRNKQGDAIIGYDRLHTFRTKEVEDGGNWTMHEYTLHPNDYNCAVCKITREEEEVVDQQPSQECPTASSDDNVAHEDLDQEDLEWGSFAETLENIILQQPEESGEACDENINNIGSDQFPPASTDDNVALENLDQEDLEWESFAETLENIVLQQPEESETPSTDDDVALEDMDQEDLDWESFAETLENTILQQPEESGEACHENINNIGSEQLPIEIDDDDVGNENMINVIAQPKGIEDTPTDKYHTDHNLPSDPLSEVFKDYEEPRGMQCHCQDNSNQVVLAEQAQQSERMALVIHLAENFDYYYKEQQQQERMAFILHLAENFDYYYKEQQQQQLQGGQKNLPGKRKKCDYDEEDFSPKKKQKQQPFLCNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
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    <name>A0A164W566_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_021339</name>
        <name evidence="2" type="ORF">DCAR_0624959</name>
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    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH05541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W566"/>
    <dbReference type="STRING" id="79200.A0A164W566"/>
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        <property type="gene ID" value="DCAR_021339"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47389:SF4">
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM91296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24634" checksum="CD0BA12CE9520DF6" modified="2016-07-06" version="1">MVNDKHVNARVRAGLEEEWCSGFERCEDTAVWESNSTSTAARKAELFNTGVSEPCKNQCFAIGKMICRCWLLLVFNGRHFEKVDVALSDGPWIMDDSLFIHPTQPLPVDRSFQLRPHSNLFKIVPDDKVIALSRTKMNPHFFDIAVGHFSMKHPSINCNELFCVKGIDGNKYNGGPVINFSGEVIGIMSHGSSFLPSNIVSRWWKHYRTYRSDSVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z502</accession>
    <name>A0A164Z502_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Root phototropism protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_018580</name>
        <name evidence="8" type="ORF">DCAR_0521262</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Z502"/>
    <dbReference type="STRING" id="79200.A0A164Z502"/>
    <dbReference type="Gramene" id="KZM95338">
        <property type="protein sequence ID" value="KZM95338"/>
        <property type="gene ID" value="DCAR_018580"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223808"/>
    <dbReference type="OMA" id="KMKMYIA"/>
    <dbReference type="OrthoDB" id="624345at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000168">
        <property type="entry name" value="BTB/POZ domain-containing protein At1g03010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="33"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="189"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM95338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH01876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="64355" checksum="517EB755CB85DDE5" modified="2016-07-06" version="1">MAASTATFKNTATRLSLAMERTNQWVFSQEIPTDLLVEVGGASFNLHKFMLVAKSNYIRKLIMDKKDGTELERIDLSDIPGGAEIFEKAAKFCYGVNFEITVHNVAALRCASEYLQMNDKYCDGNLAGRTEDFLSQVALTSLSGALVVLKSCENLLPLAEDLNIIKRCVDVATTKACNEANFPSRSPPNWWTEELSILDICFFGKVITSMKGRGAKGLTIASAIITYTERSLPDLVRDHSGSNAIKQESSNIRVQQRELLESIVALLPPDKAVFPISFMCCLLRTAIFLGASNSCKTELEKRISAILEHVTVDDLLITSYTYDGEKLFDLESVRRIIAGFVDKEKNVAVFNGGDFKEVCSAATQRVAKTVDAYLGEIAVHSELSISKFNGIANLVPKAARKVDDDLYRAIDIYLKAHPNLDEIEREKVCSVMDPLKLSYDARLHASQNKRLPVQIVLHALYYDQLKIRSGVDDRKAPDAISTRNQLQADVSLARENEALRTELLKMKMYLSDMQKSGSSNSLGTTSAATKGVKKQTKFFSSMSKTLGKLNPFKHGSKDTSHIDDVGVDLTKPRRRRFSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZB79</accession>
    <name>A0A164ZB79_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0521612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZB79"/>
    <dbReference type="Gramene" id="KZM95653">
        <property type="protein sequence ID" value="KZM95653"/>
        <property type="gene ID" value="DCAR_018895"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220639"/>
    <dbReference type="OMA" id="WNFIKSC"/>
    <dbReference type="OrthoDB" id="19610at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48146">
        <property type="entry name" value="K-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48146:SF2">
        <property type="entry name" value="K-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH02223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="357" mass="41426" checksum="92242252F8680995" modified="2016-07-06" version="1">MPPHTLTPRSSAYLTALTQEIEKKLQRGIASPTLSRNLLQELFQDIALEVDDRAKDIILSNEELASIEEERTEGPLCFYEVLADHFVWVPKNGEPILDLIVKLWSQAFASHIFSLLFHKWLFEVQIDNADVLLRYSSALVQGATNVFWIDVQTNTRHFHSLFRYLLEEVALVPERLKKIPLQAQRDLFLLLSRFIFLYNMVDKIESFLKKFPEFPNAFLIGGPADIFVIELTDQLLKLKVEPVLLHYLSHIKALQGMELRMATSTRLKTCLYSFTSPGGPMYPTRAVRHAAWVALDLLFPVGQYPRHVISLFFRLLYPWYWPSSCWNFVISCVQAVFYSVLRLLFSSWDRLTKTRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZPZ8</accession>
    <name>A0A164ZPZ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_019470</name>
        <name evidence="5" type="ORF">DCAR_0522274</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZPZ8"/>
    <dbReference type="STRING" id="79200.A0A164ZPZ8"/>
    <dbReference type="Gramene" id="KZM96228">
        <property type="protein sequence ID" value="KZM96228"/>
        <property type="gene ID" value="DCAR_019470"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220734"/>
    <dbReference type="OMA" id="VSCANVV"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF577">
        <property type="entry name" value="CARBOXYLESTERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="82"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM96228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="324" mass="35810" checksum="9BEDF45615DA7B64" modified="2016-07-06" version="1">MDKAFPTEVSSESEVVHDLPPWLRVYKDGKIERLSGCGVCAPGLDSQTDVLSKDVVIVQETGVSARLHRPNVIPKGQKLPLVLYFHGGAYLISSPWDPTYHNCVNKLVAEAKVVLVSVDYRKAPEHPLPVAFDDSWDVLKWAALHTSSGAGSEPWLLDNVDFNRVFLAGDSAGASIAYQLVIRNKTEKLMMKLAGIILINPYFWGNEPIGAEVTNPFLKALVDKWWAFVCPSDKGNDDPLVNPLAAGAPPLEQLECAKILVSVSEKDILRDRGVLFYESLKKSEWKGKAELIDVKGEDHVFHIFNPDCENAIYLFKGLAAFINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XIT5</accession>
    <name>A0A165XIT5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XIT5"/>
    <dbReference type="Gramene" id="KZM98211">
        <property type="protein sequence ID" value="KZM98211"/>
        <property type="gene ID" value="DCAR_014427"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217938"/>
    <dbReference type="OrthoDB" id="2161780at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102705">
        <property type="term" value="F:serine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006580">
        <property type="term" value="P:ethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006563">
        <property type="term" value="P:L-serine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051151">
        <property type="entry name" value="Group_II_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002748">
        <property type="entry name" value="PRK02769.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46101">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46101:SF9">
        <property type="entry name" value="HISTIDINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG97162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="52007" checksum="FA7F025C16F0EF16" modified="2016-07-06" version="1">MVGSIGLESTTILSVEWNTDMKEVGEELNPTSKKEIVKVNKNAHLRCLPITEPDVDDEITGEKDAYMTQVLAQYQKSLVQITSNQLGYPVNHEYDYGALGQFQHFSINNLGDPFMDSTYGVHSRQFEIGVLDWFARLWEIERDEYWGYVTNCGTEGNLHGILVGREVFPNGILYASEDSHYSVFRAARMYRMDCVKVRTLLTGQIDCADFKSQLLANQGRPAIVNVNIGTTVKGAVDDLDLVIQTLEECGLSDDKFYIHCDGALFGLMIPFVDSAPKVSFKKAIGSFSVSGHKFVGCPMPCGVQITRMKHIKALSSNVDYIATRDATIMGSRNGHSPIFLWYTLNKKGYKGFRNEVQRCLKNAHYLRDRLKEAGISAMLNELSSTVVFERPRDEDFVRQWNLVCLRNMSHIVVMPNVTVDKLNSFVKELTEKRSVWYQGQKVQPPCLAADIGKGNCECALCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A5R3</accession>
    <name>A0A166A5R3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0310768</name>
    </gene>
    <organism evidence="1 2">
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Ashrafi H."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D1E3"/>
    <dbReference type="Gramene" id="KZN04610">
        <property type="protein sequence ID" value="KZN04610"/>
        <property type="gene ID" value="DCAR_005447"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN04610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="27574" checksum="319B4E8EED788728" modified="2016-07-06" version="1">MQDNSGARQRASASMGQGCLVQRNLGEILVESGTHARFGAEIDKHFEEKACIERFVNSLTELHKAYKKCIADCVSIFVLFPESESLAKLKAEYPMFFNLFAETSPMAKNMCLRSICVGRNVEVMDDDDSFVPNYSLGISQISPKNLEKNIEGANAGCSDKKYIEKNREHSRRMGKVVFDKDGYVRQRRGLAPSRICRSPFVTRVTDVNAHRITVEERDFCDWLMQDEQDSGYNMQRYTILVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EPS7</accession>
    <name>A0A166EPS7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0208656</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EPS7"/>
    <dbReference type="Gramene" id="KZN06827">
        <property type="protein sequence ID" value="KZN06827"/>
        <property type="gene ID" value="DCAR_007664"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209892"/>
    <dbReference type="OMA" id="MANEHED"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG89418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="48134" checksum="C7985B1CAFD08233" modified="2016-07-06" version="1">MAPPPAESIEDEIRNEKNPRPLDEDDIALLKTYGLGPYSNTIKNAEKEIKEMAKKINDLCGIKESDTGLAAPSQWDLVSDKQMMQEEQPLQVARCTKIINPNTEDAKYVINVKQIAKFVVGLGDKVSPTDIEEGMRVGVDRNKYQIQIPLPPKIDPSVTMMTVEEKPDVTYNDVGGCKEQIEKMREVVELPMLHPEKFVKLGIDPPKGVLCYGPPGTGKTLLARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFQMARSKKACIVFFDEIDAIGGARFDDGVGGDNEVQRTMLEIVNQLDGFDARGNIKVLMATNRPDTLDPALLRPGRLDRKVEFGLPDLESRTQIFKIHTRTMNCERDIRFELLARLCPNSTGADIRSVCTEAGMYAIRARRKTVTEKDFLDAVNKVIKGYQKFSATPKYMVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FEB7</accession>
    <name>A0A166FEB7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0209627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FEB7"/>
    <dbReference type="Gramene" id="KZN07685">
        <property type="protein sequence ID" value="KZN07685"/>
        <property type="gene ID" value="DCAR_008522"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208965"/>
    <dbReference type="OMA" id="WKCQAME"/>
    <dbReference type="OrthoDB" id="567788at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF190">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="247" mass="27423" checksum="A745E6F9DB0C532E" modified="2016-07-06" version="1">MSDHEESMIEKISEKIKGDDSSSSSSDSSDSETETTTLKEKVFRIFGREKPVHKVLGGGKPADVFLWKNKKVSGAILGGATAIWFLFEVMEYYFLTLVSHALILSLAIMFLWSNATTFINKSPPKIPEVSIPEAPVKEIAAALRVEINRAFAVIRQIALGKDLKQFLSVVAGLWILSILGSCFNFLTLCYIGVVSLFTVPFLYDKYEDKIDPLAEKATIEIKKQYAVFDAKVLSKIPKGPLGKKKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G4Z3</accession>
    <name>A0A166G4Z3_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_001069</name>
        <name evidence="2" type="ORF">DCAR_0101095</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166G4Z3"/>
    <dbReference type="Gramene" id="KZN08539">
        <property type="protein sequence ID" value="KZN08539"/>
        <property type="gene ID" value="DCAR_001069"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN08539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="249" mass="28183" checksum="438BA3436D474B53" modified="2016-07-06" version="1">MASSSSSSSMNLKTVNVWDDSFEIHFDNFISTTRNYNVIAADTKFAGILEKIRYKKKDDVYAQMKRNIKETKCIQFDMKTDTYCPDAMESEADPDRSKREKHARDGIPTHKFCEAMLNSDLLLTGALSVYQKTWVTYGENYDLGHCVKLFQPTNVLPDSQLVFGDMVRSHFGERLFDVKLMLAYTDLAYGKMKLANVAARFNVDNGGVSYEAAADALVTLKLYHILKIKDGNVNCVNMVTDLFYGATYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I2N3</accession>
    <name>A0A166I2N3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN10680">
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        <property type="gene ID" value="DCAR_003336"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209948"/>
    <dbReference type="OMA" id="MPIRTEG"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000003">
        <property type="entry name" value="Trafficking particle complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="15962" checksum="364F076E25F78182" modified="2016-07-06" version="1">MPAVYSLYIINKSGGLIYHKDYGSAGRMDTNDSLRLASLWHSMHAISQQLSPVAGCTGIELLEADTFDLHCFQSLTGTKFFVVAAPGTQNMESLLKHIYELYTDYVLKNPFYEMEMPIRCELFDINLTQAIQNDRFALLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WEB9</accession>
    <name>A0A169WEB9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0313639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WEB9"/>
    <dbReference type="Gramene" id="KZN03804">
        <property type="protein sequence ID" value="KZN03804"/>
        <property type="gene ID" value="DCAR_012560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="17145" checksum="6A30595B5D700B4F" modified="2016-07-06" version="1">MEQPPRRTTPRSAPRGKSAAASTSKAPRSLSGSPLRPGYTYRAVSGSTSRTPRLTGAYTSRAYAGASSSRSAGGPSSGLIRQAVPVPHEEHEVEVVYTGLPCTCKNCGITFGSYHDYYCHLVYAAIHQAPPLDLTLKLATTCQLPKVDLELRLRPADQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WKY7</accession>
    <name>A0A169WKY7_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_005655</name>
        <name evidence="2" type="ORF">DCAR_0206359</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WKY7"/>
    <dbReference type="Gramene" id="KZN04818">
        <property type="protein sequence ID" value="KZN04818"/>
        <property type="gene ID" value="DCAR_005655"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN04818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9620" checksum="7E30D4412E699E0A" modified="2016-07-06" version="1">MRSVRKNSKKIGGNIYEILKNRAFRILRNLEILAGKRRIKLQDGSSFVDEDSKVTVYQNVQWSSRYQVVVRPNGTDVNFFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WTS4</accession>
    <name>A0A169WTS4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_002535</name>
        <name evidence="3" type="ORF">DCAR_0102649</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WTS4"/>
    <dbReference type="Gramene" id="KZN09879">
        <property type="protein sequence ID" value="KZN09879"/>
        <property type="gene ID" value="DCAR_002535"/>
    </dbReference>
    <dbReference type="OMA" id="KPYLIEC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="94"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN09879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="345" mass="39715" checksum="C204CC9373593E67" modified="2016-07-06" version="1">MSDVNTMSDFWSKLPQDLLRLLVDRYVVSEKDFLCLRSVCRDWRSALPKSCWKRTPWLLARRYQTPNVVIKGLDSLCSGHGSLSSLFVTDLSWRYWGSFTGWILGQNHSDYTLKLINPLTKAVIDLPELHHYLGKGVVYHAPGSDPRNPVIGVMAVSHSFSGIAMIDNECKEWTFLKDKRYVSNSDFKDLIWYKDNVAAVRGDGEIVFFDEKRVVRSFNPKRTLVREYYDHYLVESSGDLLIVANVEHRYEVYRLSLKTGGWTRVWDLGRHSLFVGKSYSMSHWISADTTGDSWRPSCIYDATYSPTGSCYNIAKKQQECHDVSSFDGLNKCFDFIWYMPAVGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YAW9</accession>
    <name>A0A175YAW9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YAW9"/>
    <dbReference type="Gramene" id="KZM80378">
        <property type="protein sequence ID" value="KZM80378"/>
        <property type="gene ID" value="DCAR_032420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="72" mass="7908" checksum="DF23383921A60D27" modified="2016-09-07" version="1">MASSSNHSASNSVTQPIYTENVEKSLCNLNGHLATLREQMVELTQLTATLNKMVELLGDDLKAVMMEQSQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE33</accession>
    <name>A0A175YE33_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 19">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0933810</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Lipoamide dehydrogenase is a component of the plastidial pyruvate dehydrogenase complex (PDC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 19">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="17 19">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Part of the plastidial pyruvate dehydrogenase complex (PDC) containing multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="19">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 19"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="240" mass="25744" checksum="5F562798B73E0354" modified="2016-09-07" version="1">MEMEIDNPEEIKADEVFKAAESGDSSVFDSLSPQNLLKILSFRNEDQRSLLHVAASSGRTQVVKILSAFDPLVSGINSADEEGWVPLHSAASSGNVEIVELLLTRGADVNLKNNGGRTALHYASSKGWLKIAQLLIAHNAKLNVKDKVGCTPLHRAASTGNGELCEFLIEEGAEVDAVDRAGQTALMTAVICDNREVSLLLIRHGADVDVEDKEGYTVLGRASNDLRPLLIDAAKAMLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQ76</accession>
    <name>A0A175YQ76_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_027081</name>
        <name evidence="4" type="ORF">DCAR_0832494</name>
    </gene>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH12985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM85497">
        <property type="protein sequence ID" value="KZM85497"/>
        <property type="gene ID" value="DCAR_027081"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199889"/>
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    <dbReference type="OrthoDB" id="270970at2759"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006952">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF173">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="191"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM85497.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="31579" checksum="1D8D03443ADDD991" modified="2016-09-07" version="1">MEYRTLDLKIISAKGLKNVNFISKVEVYAVVSIAGGDPRVKQRTPTDREGGVNPAWNFPMKFTIDEAAAQQNRLTLVISLRAERALGDRDVGEVRVPIKEIIGDGNNKSVQFVRYQVRKPSGKAKGELNFSYQFGEKVAASHVPPQANHVTSKTDEPVTAYPAVAPMAAGTSSAYPPPVTSAYPPVASSGYPPAAYPPQQPGHGYPPQQPGYGYPPPQPGYGYPPQQPGYGYPPQQGYGYGAPVQQPPKKNKFGGGGMGLGLLGGALGGLLIGDMISDVGSYDAGYDAGFDDAGGFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQV1</accession>
    <name>A0A175YQV1_DAUCS</name>
    <protein>
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        <alternativeName>
            <fullName evidence="10">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0832164</name>
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    <organism evidence="13 14">
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQV1"/>
    <dbReference type="Gramene" id="KZM85202">
        <property type="protein sequence ID" value="KZM85202"/>
        <property type="gene ID" value="DCAR_027376"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199513"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000132">
        <property type="entry name" value="Aspartyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH12657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="534" mass="59837" checksum="686B27D997BF1614" modified="2016-09-07" version="1">MDPQEDQTKKPSKKEAAKAERLRRKQELAAASAAAAGVSSVSIDEADPLASNYGEIPLIELQSKSIAVQQWTEIGSLGESLKESSVLIRGRVHSIRAVSKKMVFVVVREKGFTVQCVLTVKPEVVSLQMVKFVTNLNKESFVDVEGVVSVPASPLKATTQQVEIQVSKVYCVNKALPTLPINIEDAARSESEIEAALQAGEQFVRVNQDTRLNNRVLDLRTPANQGIFRIQYQVEKIFRHFLEDEGFVGIHTPKLMGGSSEGGSAVFRLQYKGGVPACLAQSPQLHKQMSICGDFARVFEVGPVFRAEDSFTHRHLCEFTGLDVEMEIKQHYSEVMDIVDRLFVAMFDHLKQNCQKLLEAVGKQYPFEPLVYLPKTLRLTFQEGVQMLKEAGVEVDPLGDLNTEAERRLGQLVFEKYKTQFYILHRYPLAVRPFYTMPCPDDNAYSNSFDVFIRGEEIISGAQRVHVPEFLEERAKACGIDVKTISTYIDSFRYGAPPHGGFGVGLERVVMLYCNLNNIRKTSLFPRDPQRIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0VZS0</accession>
    <name>A0AAF0VZS0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-quinone oxidoreductase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0100082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0VZS0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG80937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16371" checksum="3BAF6F3F909C7C62" modified="2024-05-29" version="1">MTISFKIWFLGVEFVASTYRVYSYQTEYSGIKFGLFYVASYLNLLVSSLFVTVLYLGGWNLSLPHIALPFFFEINKAGRVFGTIIGIFITLAKTYLFLFIAITTRWTLPRLRMDQLLNLGWKFLLPISLGNLLLTTSSQLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3U7</accession>
    <name>A0AAF0W3U7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W3U7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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    </evidence>
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    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
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                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Ashrafi H."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
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        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="960"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
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            <begin position="961"/>
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        </location>
    </feature>
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</entry>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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            <end position="665"/>
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            <begin position="267"/>
            <end position="288"/>
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        </location>
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            <begin position="137"/>
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        </location>
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            <begin position="330"/>
            <end position="617"/>
        </location>
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        </location>
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        <ligand>
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    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9X1</accession>
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        <lineage>
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        </lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    </reference>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="237" mass="26468" checksum="B8C8553F0B039968" modified="2024-05-29" version="1">MKNPMSKVIMPRFYLVFLFMHGIFEMVNFSTARSQDHIFKAPPVSSSLLCYSNCATCPFICSPPPPAPPAMPSKSPRHLPLPPISLSPPEPYNFDQPPPLPHINLSPPESNYFDQPPPLPRITFSPPEPYYYDQPPPLPPINFSPPEPYIFDQPAPLLPAFPPPPPPYAAAWSNNPYAVSPPVPPNYVAGQGPPGTGQQRSFTFPYYYYNISQGCCISFHFAFLFHFIVCLAMFLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WB18</accession>
    <name>A0AAF0WB18_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0205403</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WB18"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF22">
        <property type="entry name" value="CYTOCHROME P450 83B1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG86202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="57611" checksum="7A068B9B4A75D1F1" modified="2024-05-29" version="1">MALVYLCLLVGIPVLCIILLQKLLGNKNSKLPHPPGPRGLPIIGNLHQIDVSIFPQHLWELSKQYGPIMYLRLGHLPTLVISSPEIAKEFLKTHDLNFCSRPYLFGLKKVSYNGRDVLFSPYNEYWREMRKIVTVHLFSSKRIQSFRSIREEEVFQMIKGISEKGSSNEVLNLSDTLAPLTIAMTSRLAFGKKFNEVDMKRFKGLLKRFQDVTAAFYFSDIFPSLQWLDRFTGSSAELDGCFRDMDSFYQEVIDEHLDPCRPSSMDGDVIDILLQLKRDDQISSIDFTFDNIKAIIMNIFFAGTDTTANTLLWIMTSLIKNPEVMHKAQEEVRKLMHGKERIEEEDLQNVKLPYLEAVIKETMRMYPVAPLIPRESLDRMVVNGYEILPKTIVYVNLVAIGRDPLVWENAEEFFPDRFLDTDIQFKGHDFEFIPFGTGRRICAGMYMAVATLELVLSNLLYSFDWELPPGIRKEDIDTDIGRGITLHKKNALCLKPRIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBD9</accession>
    <name>A0AAF0WBD9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0206028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBD9"/>
    <dbReference type="KEGG" id="dcr:108205595"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF5">
        <property type="entry name" value="N-ACYLPHOSPHATIDYLETHANOLAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="61"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG86810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="283" mass="31524" checksum="941255C8F76ED47F" modified="2024-05-29" version="1">MGGHMEWAASANHLKGVPRKLVIGGVGLFAKTVATLLNHTNVHNSHTLLRLLRSRPPGVPLITVSNHMSTLDDPVMWGFKGFPTSDASLARWALAAEDICFKNTVLSYFFRLGKCIPITRGGGIYQEHMNEALDRLSDGEWLHTFPEGKVSQEDAPIRRLKWGTASLIARSPVTPIVLPIVHRGFEKVMPEDYLFGRRPPFPLCNKRVEIIVGDPVEFDIPNMKEMAISLSRDTSLPLIGWPSTADGLDEPAQRHLYTTIADQIRTVMERLRSLSKSNSELKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDI7</accession>
    <name>A0AAF0WDI7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0206781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
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    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
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    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
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        <property type="entry name" value="OS12G0117600 PROTEIN"/>
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            <begin position="491"/>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNE5</accession>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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            <begin position="22"/>
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            <begin position="176"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="309" mass="34532" checksum="02B2E626F815D591" modified="2024-05-29" version="1">MGSNAQVKQVAFKSNNTTVNVRWGSKEDATLVKSLLKLVDDGGWRAENGQLKSGAYGKLEKIMGELLPGCGMKARPHIESRVRLLRKQFFAIEEMRGPNCSGFGWNELEKSITCEKSIFEEWLKSHPNAKGLRNKSFPFYDELAQVFGKDRANGEGVESPADAVEEIANDEESNLYQQAGQQKDNLEDEVSPRNVQPTDTGARGSKRIKTDSLEIVKELTFGLQKISNVMEIGNENIAKLTSCFQHESDGANKRMMVNSELLKIEGLSPDQFIKAGRKIALDPLETDYFFSLPEDYRSTYVQALLLPDS</sequence>
</entry>
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            <fullName evidence="5">DUF4005 domain-containing protein</fullName>
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        <name evidence="6" type="ORF">DCAR_0311216</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <tissue evidence="6">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN IQ-DOMAIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="721"/>
        </location>
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            <begin position="324"/>
            <end position="368"/>
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    </feature>
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            <begin position="555"/>
            <end position="734"/>
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    </feature>
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            <begin position="332"/>
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        </location>
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        <location>
            <begin position="700"/>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPS5</accession>
    <name>A0AAF0WPS5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0311200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPS5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000039">
        <property type="entry name" value="Villin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000028">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000038">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF138">
        <property type="entry name" value="VILLIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="10">
        <location>
            <begin position="881"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="799"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="816"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG91945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="946" mass="105969" checksum="CC3CEC5EC133253C" modified="2024-05-29" version="1">MAVSMRDLDSAFQGAGQKAGLEIWRIESFRPVPVSKSLHGKFFTGDSYVILKTTALKSGAVRHDIHYWLGKDTSQDESGTAAIKTIELDAVLGGRAVQYREVQGHESDKFLSYFKPCIIPQEGGVASGFDHTEAEEHKTRLYVCRGKHVVQVKEVDFGRSSLNHDDIFIVDTTSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADAETGEFWGLFGGFAPLPRKTATVDVNDTGDVSTRLFGVVKGQHEPLDVESLKREQLDTYKCYLLDCGSEIFVWMGRNTSLDERKSASGAAEELLHTVHRPKTQVVRVMEGFETVMFRSKFNSWPQSTDVTVSEEGRGKVAALLKRQGVNVKGLLKATPEKEEPQSYIDCTGNLQVWRMDAEGKIPLTGSSMSKFFSGDCYIFQYTYPGELGDQYLVGTWFGEQSVEEDRNSATLQASKMVESLKFLPVQARICEGNESIQFFSIFQSFLVFKGGVSANYKMYIAEKELPDVTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSHGYILHNGSSLFTWLGNLTTSEDQELVERLLDLIKPNVQPKPQKEGSESEQFWDMLGGKSEYPSQKVGRDVESDPHLFSCSFTNDLKVKEIYNFDQDDLMTEDIFILDCHSEIYVWVGQQANSKYRRDALTIGQKFLECDFLLEKLCGQAPIYIITEGSEPTFFTRFFTWDSTKSAMHGNSFQRKLAVVKNGGTPLLDKPKRRTPVSYGGRSAALPEISQRSRSVSFSPERVRVRGRSPAFNALAAAFENSNTRNMSTPPPIVKKLYPKSGSADSAKSVSRSSPITTLTSSFDKPAEDTSIPHLVKESKKAPINNITEAITIQEDVKEGEADDDEELVTYPYERLTKLSNNPVTDIDVTKRETYLSSKEFREKFKMAKAAFYKLPKWKQNKLKMALQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTL1</accession>
    <name>A0AAF0WTL1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0314148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTL1"/>
    <dbReference type="KEGG" id="dcr:108215002"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF684">
        <property type="entry name" value="COMPASS-LIKE H3K4 HISTONE METHYLASE COMPONENT WDR5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="6">
        <location>
            <begin position="14"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG94851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="307" mass="33622" checksum="260BC2D5E3EFB0AF" modified="2024-05-29" version="1">MSTPSPRPYRHHKTLTGHSLAISCVKFSPTGKLLASSSLDKTLIIFSLQSLSPLSTLSGHSAGISDLAWSSDSHYICSASDDSTLRIWDALLFDCVKTLRGHSNYVFCVNFSPRSDMIVSGSFDETVRVWDAKTGKVVHVIQAHSMPVTSVGYNRDGSLIVSGSHDGSCKIWDNESGKCVKVLIDDKTPAVSFAKFSPNGKFILVATLDDTLKLWNHQTGKFLKIYTGHLNRTYCITPTFSVTNGTCIVSGSEDKCVYIWDLQGKSVIQKLEGHTDTVVSVSCHPTENMIASAGLDLDRTIRIWVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTY6</accession>
    <name>A0AAF0WTY6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">pyruvate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0415382</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-oxocarboxylate + H(+) = an aldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:11628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="EC" id="4.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTY6"/>
    <dbReference type="KEGG" id="dcr:108217920"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036293">
        <property type="term" value="P:response to decreased oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000009">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000017">
        <property type="entry name" value="pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000021">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF6">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="17">
        <location>
            <begin position="44"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="15">
        <location>
            <begin position="241"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="16">
        <location>
            <begin position="451"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG96052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="605" mass="65270" checksum="EA53F19CBE36647E" modified="2024-05-29" version="1">MDTSISTVTTSPLACNDLGSPPKNSAVSPIHDTPTTSINSSQGTLGRHLARRLVQIGVNDIFSVPGDFNLTLLDHFIAEPGLELVGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVINAIAGAYSENLPVICIVGGPNSNDYGTNRVLHHTIGLPDFSQELLCFQTVTCFQALVNNLEDAHELVDTAISTALQQSKPVYISVGCNLAAIPHPTFSREPVPFSLSPKLSNKMGLEAAVEATAEFLNKAVKPVLIGGPKMRVAKACEVFVELADACGYPISVLPSAKGMVPEHHPHFIGTYWGAVSTSFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIILQPDRVVIANGPTFGCVLMKDFLQALAKRIKHNTTAFENHCRIYVPEGLPPKSAPEEPLRVNVLFQHIQNMLSGDTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQADKNKRVIACIGDGSFQVTAQDVSTMLRWGQNPIIFLLNNGGYTIEVVIHDGPYNVIKNWNYTALVDAIHNGEGKCWTAKVRCEEDLVEAIKTALGDKKDCLCFIEVICHKDDTSKELLEWGSRVSAANSRPPNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WV17</accession>
    <name>A0AAF0WV17_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0414471</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WV17"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000007">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF0">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="4">
        <location>
            <begin position="167"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG95168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="31938" checksum="470F024D10C7EC99" modified="2024-05-29" version="1">MAYLYPDKPNHQLGLAPRENFSSLDEFFAQFKYTGGRVEKFGQSSNTNVEEKPKTTPAQVCVVSRSLGNNNSEGTSNKQGKSQVVRMPPKTCGKRSWAEVSVVKEKEDVPKRGKSPRRKMPTLTASQKVDEAYQRVGPGNTWKPPRSPHNLIQEDHAHDPWRVLVICILCNKTNGKQVKKVISDFFKLCPSAKAATEVPVEEITKLTISLGLQNTRARKIQRLSSEYLEENWTHVTKLFGVGKYGADAYAIFCTGKWDRVTPEDYMLEKYWEFLHGNKVTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WXL7</accession>
    <name>A0AAF0WXL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0416844</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WXL7"/>
    <dbReference type="KEGG" id="dcr:108219177"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="38"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="217" mass="24438" checksum="998A74D6E8FD214C" modified="2024-05-29" version="1">MATRVKARSELIPYLVFIIYVITSFGGAVWLEVPSTGTKCVSEDIKQNVVVIADFSVVNDDSNPHNTFNIAARVTSPYGNNLYHQENVTHGQFAFTTTEAGNYLACFWIDESHKATTGVSVSLDWRTGIAAKDWESVARKEKLEGVELELRKLEGAIDAIHQNLIYLKSREADMREVSEITNGRVAWYSIMSIGVCIAVSVMQVFHLKRYFRKKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY24</accession>
    <name>A0AAF0WY24_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0415954</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY24"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG96618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="578" mass="63706" checksum="E1AAB738640DBB30" modified="2024-05-29" version="1">MSLTVPDAVDYKGYPADRAKTGGWVPAALILGIEICERLSTMGIAVNLVTYLGGVMHVPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTAALALSTKLRQFRPPPCQNTALNNCKQANEFQMGALYVALYLIALGTGGLKSSVSGFGTDQFDENNEREKAQRAFFFNRFFFFISMGTLMAVTVLVYIQDEVDRSLGYGLCSVAMIVAILIFFSGSRRYRYKKSVGSPIVCIFQVIAAATRKRKLELPYNVTFLYEDSPEASRIHHTDQFHFLDKAAIVAEGDFDKSCPNSAPNPWKLCSVTRVEEVKMMARLLPIWATTIIFWTAYAQMITFSVQQASTMDRYMGSFQIPSGSLTVFFVAAILITLAFNDRVIMPLWKKWKGKPGLSSLQSIAVGLIMSTIGMGAAALVEMKRLSVAEDAGRNTPVLPISVFWLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFLSSFLVSVVKKVTGTSDGHGWLADNINFGRLDYFYGLLAILSAINFVIYIICAMWYKPQKAKPVVEMDDMGKNILPEDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZL8</accession>
    <name>A0AAF0WZL8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photosystem I reaction center subunit V, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PSI-G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0417242</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="15">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
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    </dbReference>
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    </dbReference>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
        </source>
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        <property type="project" value="TreeGrafter"/>
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        <location>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG98186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="486" mass="53626" checksum="E1568AB0D3A99690" modified="2024-05-29" version="1">MEGSVDENLLTEVRRAEEEGTLKDRVWTESKKMWIVAGPAIFSRFSTFGVGIITQAFIGHIGPSELAAYALVSTVLLRFANGILLGMASALETLCGQSFGAKRYDMLGVYLQRSWLILSICSIILSPVFIFTTPILKALGQEESIAEVAGTISLWLIPGLFSFMVSYTCQMFLQAQSKNKIIAYLAAFTLVFHVFLSWLLTVKYKFGLSGAMVSTILAFWIPNIGQLVYIFGGWCSETWKGFSMLAFKDLWPIIKLSLSSGVMVCLELWYNTILVLLTGSMRNADIAIDALSICININGWELMISLGFLAAASVRVSNELGRGSSRAAKFSIMQTVLTSFAIRFVLFIIFLFLRGRLAYIFTESTEVAAAVADLSPLLACSILLNSIQPVLSGVAVGAGWQSTVAYVNITCYYLIGIPVGVVLGYVLQFQVKGVWVGMLFGTLTQTIVLIIITYKTDWEKQVLLARQRINKWSVKSEEAEEDEQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0B0</accession>
    <name>A0AAF0X0B0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0518758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X0B0"/>
    <dbReference type="KEGG" id="dcr:108223114"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000416">
        <property type="entry name" value="p-loop nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056653">
        <property type="entry name" value="DUF7751"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF39">
        <property type="entry name" value="AAA-TYPE ATPASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24933">
        <property type="entry name" value="DUF7751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="927"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1121"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG99410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1195" mass="130055" checksum="2C80410FAC2D5673" modified="2024-05-29" version="1">MVSTRRSGSLSNNNKRSSSSDEKLPSPKRQKAEKATTSEKPTAVEDSKEVAAPVQVDPQECVAADSAVASREAVIEAKPDVAPEASVATPVAPGTTPTVGEKPKSSFTSWKQNQSTTMSAPWCRLLTQNQLNPTMSVYTTNFLVGTSNNANLLLNDHTISGILCAIKLTQRGSSAIAVLESKNGKGFVQINGKTIRKNATCDLHSGDELVFGLLGAHAYIFQQLVCDVIVKTPSLVGGPNFKLVQAEKREGEISAAGATLLATLSSMRQDISRMRSTAQSISKTYQTTELPSSPVLEEELDALEVNSSTNADSDSAVDIGATTKILASDWNVDGGIETGTMTEEKGWGKDVKPLNPSAACLRYAILKDDIYKGVLDGRDVKVSFDDFPYYLSENTKDVLIAASYIHLKHKEQVKFTAKLPTINPRILLSGPAGSEIYQEMLTKALANYYGAKLLIFDSHSILGGLSAKEAEHQKEGKSVDKSNNTTKQNTVASVAAQSLGDSSAEKDIPSTSKVSVAKSLESQPKMKTETMPSPSGTLKNHSFKTGDKVRFVGAVSASLYATPSPARGPTSGYKGKVVLPFEDNPSSKIGVRFDKPIPDGVDFAGLCDSGHGYFCNAKDLHLESTGRDDPNKLLINTLFEVVFSESRSSPFILFIKDVEKSVVGNTDTHSLCRSKLERLPDNVVVIGSQTHGDNLKEKSHPGGLLFTKFGSSQTALLDLAFPDSFGRLHDKGKEVAKATKQLTRLLPNKVTIHMPQDESLLAGWKQQLDRDSETLKMKGNLNCLRTVLFRCGLECDTLETLCITEQSLTIESAEKVVGWALSHHLMQNPKADPEARLVLSGDSIQYGIGILESVQNESKSLKKSLKDVVTENEFEKKLLAEVIPPNDIGVTFEDIGALENVKDTLKELVMLPLQRPELFCKGQLTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINISMSSITSKWFGEGEKYVKAVFTLASKIAPSVIFVDEVDSMLGRRENPGEHEAMRKMKNEFMVNWDGLRTKDTERVLVLAVTNRPFDLDEAVIRRLPRRLMVNLPDAPNRAKILKVILAKEDLSADVDLDSVANMTDGYSGSDLKNLCVTAAHRPIRELLEREKKEQSAAQAEGKPTPALSSSADIRPLNMDDFRNAHEQVCASVSLESQNMTELQQWNELYGEGGSRRKKSLSYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X231</accession>
    <name>A0AAF0X231_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X231"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF18">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE CPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="290" mass="33278" checksum="F764AF5D36523A9F" modified="2024-05-29" version="1">MESQSSSSSQTTWFTYFVAFLRKCIFMVLSRGPIPSHIAFIMDGNRRFSKKNKLMEGAGHRVGFSALMSMLRYCYEFRVKYVTIFAFSIDNFNRRPEEVQSVMDLMLEKIEGLTKQESLVNQYGVKVRFVGNLQLLSRPVRLAAERAMVATADNSKAVLTICVAYTSTDEILHSVQESCEEKKEELSALEATGSGPYGLLGPNSNDKEHKECFINSKDIEKHMYVTPDPDIIVRTSGETRLSNFLLWQSSSTLLYSPLVLWPEIQFWHLVWAVIDFQRNYLYLMGKLKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3M5</accession>
    <name>A0AAF0X3M5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0520341</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3M5"/>
    <dbReference type="KEGG" id="dcr:108223701"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000028">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000019">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000465">
        <property type="entry name" value="Heat shock cognate 70 kDa protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH00963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="71255" checksum="A520D1D618EEDD62" modified="2024-05-29" version="1">MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDMKLWPFKVVPGAGDKPMIVVNYKGEEKQFAAEEISSMVLTKMREIAEAFLGKTVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIAYGLDKKSSSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDIGGNARALRRLRTACERAKRTLSSTAQTTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKNSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVFEGERTRTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAERYKSEDEEHKKKVEAKNALENYAYNMRNTIKDEKISSNLGAEDKKKIEDAIEQAIQWLDGNQLAEVDEFEDKMKELEGICNPIIAKMYQGGAGGMGGMGATMDEDGPAGGAGAGAGPKIEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X522</accession>
    <name>A0AAF0X522_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase Pif1-like 2B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0519804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X522"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF57">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="1">
        <location>
            <begin position="229"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH00444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="468" mass="53522" checksum="3D8521D3E54AAF13" modified="2024-05-29" version="1">MYPEARQYTYQEFPSHFTWLPRECRWKLCQRGEVIGRVSEVHATASDLLFLRMLLMRRKGVFSFKDIRTIDGIAYPTFKETCGTLGLLHNDKQWHDAMEENAHSSLAHQLQFSDWQLAVGDGRLPTISPGEENHESKVEILDQFVVRNEGRHPIETLFDIVYMDFESSMDSKSYLSSRAILTPTNVWVDDINVRVLEKIPGTCHTYLSQDSMEDSVSPHSDFDASFPIEYLNSLDMSCLPKHELKVKVGAVVMLMRNLNQILGLCNGTRMMILCGSQMGTRHLIPRIDMIPSDTNLPFDFTRTQFPLQVCYAMTINKSQGQSLDMVGLYLPRSVFSHGQLYVAISRVTSPSGLHILIDDDNGSSTNVTANVVFEEKLQFHGYCKLPQYLKKKIHKFLICSLMIRSNVHLCCNMFIALSLKLWKDMSIKLISSLKTESILIEGHVYNGRPSLMLIIVKTKYKSISRLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <source>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOH04393.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="18019" checksum="B46BBC53EDFBBDC6" modified="2024-05-29" version="1">MLFNSGFGWDPITKKFTAPDEVWEEYFKVHPTHKSYRTDTFGDYDDLRIAVGNGTAIGKNAIGLGDDTDARTYDNEASKKHQPLDDLVYDYNTEAYTPDDFQDPLAQPSESINLDAPPPPTLNKKRNRSEFEGNSRISFKSVFSLIILDSLRKICGYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH03674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="193"/>
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    </feature>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="336" mass="39303" checksum="EE0F99A8A1D7F2AB" modified="2024-05-29" version="1">MSGMGDGYVGTAQDAVRIRRLEKQREAERKKIQELKNKSATTKGQSGLLQFGSGTSEILETAFKKETVGLVTREQYVEKRVNIRTKIEEEEKEKLQKLQQEEEEIQMQKLKKRKIRVNPRLSFSDDLENGCEEEDGDNNNKESNNFGHRGFGKDPTVETSFLPDSEREAEEQAERERLRKQWLCEQEHIKNEPLQITYSYYDGAGHRRVLQVRKGDSIGEFLRAVQQQLAPEFREIRTASVENLLYVKEDLIIPHQHSFYELIVNKARGKSGPLFHFDVHEDVRTIADATIEKDESHAGKVVERHWYEKNKHIFPASRWEIYDPTKKWERYTIHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDL3</accession>
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            <fullName evidence="11">Solute carrier family 25 member 44</fullName>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
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    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF3">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="11"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="110"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH05157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27857" checksum="8196A11CAC216220" modified="2024-05-29" version="1">MISLSSCRLDKTKFYVVGAGIFSGLTVALYPISVVKTRLQIVTKDSVERNAFSVVRGLLKAEGVPGLYRGFGTVITGAVPARIIFLTALETTKAAAFKMVEPFKVSEPTQAAIANGVAGMTASLFAQAVFVPIDVVSQRLMVQGYAGHTSYNGGLDVARKILKSDGIRGFYRGFGLSVMTYSPSSAVWWASYGSSQRVIWRPTTRQVVRRLIADDGWQGFYRGLGPRFFSMSAWGTSMILAYEFLSIDCFSLFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEP4</accession>
    <name>A0AAF0XEP4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0625887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEP4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF16">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042153760" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH06460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="107" mass="11973" checksum="8F2D7C194BA2ADDC" modified="2024-05-29" version="1">MLGLVSVFLVLFFVYVSVEGTATLDVFSSSTVSFGSTSDRSLGATRIQVEEGNKNVQQCVICQEEYHDGEKVTCMPCLHMFHKDCITKWLLRSHLCPICKFQMPTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XGG0</accession>
    <name>A0AAF0XGG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0727053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XGG0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="75" mass="8980" checksum="CF8A7BA7A1D665E0" modified="2024-05-29" version="1">MDIYWKYETRPHCESPGSCTPTEHLRHQKSMFKSQRNALLIASALLFYWLLFTVTRLVVQIEQLNQRVEKLKNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XM96</accession>
    <name>A0AAF0XM96_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1499 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XM96"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010865">
        <property type="entry name" value="DUF1499"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34801">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34801:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07386">
        <property type="entry name" value="DUF1499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026426">
        <property type="entry name" value="DUF1499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23593" checksum="2F5402E7BCAA2F22" modified="2024-05-29" version="1">MASMAISSPAYYKSELRRAIKVSSLLPTIVSQQQKEIILRSSELALLGAIFQFSGKKPDYLGVQKNYPALALCPATKNCISTSENESDFTHYAPPWNYNPEEGRGSKKPVSKEAAMEELLQVIKSTKPDKFTPRIVEQKDDYIHVEYESPILGFVDDVEFWFPPGKKSVVQYRSASRLGNFDFDVNKKRIKALRVELEKKGWSSEDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMY1</accession>
    <name>A0AAF0XMY1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable glutathione S-transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0830522</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. HSP26 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMY1"/>
    <dbReference type="KEGG" id="dcr:108199313"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF676">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="221" mass="25409" checksum="D96C2B760571919E" modified="2024-05-29" version="1">MADQEVKLLGVWASPFSKRVEIALKMKGISYEYVAQDLSNKSPELLKYNPIHKKVPVLVHNGKPICESLVILEYIDETWKSGPSILPKDPYARAMARFWAKFIDDKCFPSIWKIRKSQGEEEREKAIEEAKEQLAILENELKGKKFFSGDEIGLVDIAANFIALWVGVVQELVGVELLTKEKFPRLCEWSDDYLNSSIIKETLPAKDELLDRFRAMFQRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQN3</accession>
    <name>A0AAF0XQN3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0831980</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQN3"/>
    <dbReference type="KEGG" id="dcr:108197872"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF431">
        <property type="entry name" value="LACCASE-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041779577" description="Laccase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="35"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="161"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="420"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH12476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="571" mass="62576" checksum="E31C8CE35154AB89" modified="2024-05-29" version="1">MEAMSSSLLHSFFFLSLLFSLANAVPRIHYHDFIVQSTPVKRLCNTHSSITVNGQFPGPTLEVNNGDTLVVKVVNRARYNVTIHWHGIRQIRTGWADGPEFVTQCPIRPGGSYKYRFTVQGQEGTLWWHAHSSWLRATVYGALIIHPKEGNSYPFPKPKRQTPLLLGEWWDANPIDVIREATRTGGAPNVSDAYTINGQPGDLYNCSSKETVIVPVDSGETNLLRVINSALNQQLFFSIANHKMTVVGADASYVKPFTTNTLMLGPGQTTDVLITGDQIPARYYIAARAYASAQNAPFDNTTTTAILEYKSAPCPAKGASIRPVLPSLPAFNDTATATAFSTSFRSPGKVEVPKEIDESLFFTVGLGLNRCPSGAMSSTCQAPNGTRFTASMNNVSFVLPSSFSLLEAHHQGIPGVFTTDFPAAPPVKFDYTGNVSSSLWQPTRGTKVYRLEYGSKVQVVLQGTSIVTAENHPIHLHGYDFYVIAEGFGNFNPETDTSKFNLDDPPLRNTVSLPLKGWAVIRFVADNPGVWIMHCHLDVHISWGLAMAFLVDNGVGELESLEHAPEDLPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTH3</accession>
    <name>A0AAF0XTH3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933461</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
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        <property type="protein sequence ID" value="WOH13948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative cinnamyl alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
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    </dbReference>
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        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="ADH_Zn_CS"/>
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        <property type="entry name" value="CAD-like"/>
    </dbReference>
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        <property type="entry name" value="ER"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="23"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="348" mass="37167" checksum="A5CCBA0AAD8098B0" modified="2024-05-29" version="1">MAKSLETEHPAKALGWAASDTSGVLSPFKFSRRATGDNDVRFKVLYCGICHSDLHMIKNEWGFTRYPVVPGHEIVGVVTEVGSKVEKVKVGDHVGVGCLVGSCGSCDSCCDDLENHCAKQVLTYSALYFDGTITQGGYSESMNLPLDSGAPLLCAGITTYSPLKYFALDKPGTRVGVVGLGGLGHLAVKLAKAFGAHVTVISTSESKKKEALEKLGADSFLISRDQEQLQDATSSLDGIIDTVSATHPLAPLLGLLKPNGKLVMVGGPEKPLEVPVFPLLVGRKILAGSNIGGLKETQEMLDFAAKHNITADVEVIPMDYVNTAMERLLKSDVRYRFVIDVANTLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVH6</accession>
    <name>A0AAF0XVH6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVH6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="136" mass="15197" checksum="B1A2DE9F37C6CA22" modified="2024-05-29" version="1">MIHSSLTQTSPQDFVDAHNAVRAVDGVGPIAWDETVANYAKNYAAKIGPTCLMQHSGGPYGENLFKGGGDATAKQIIDYFASEKKYWNNNTKTFLFSFMKCEDIQFSIRILKHVSIGIFNQDSKLNYKSRGLQYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVQ7</accession>
    <name>A0AAF0XVQ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <location>
            <begin position="106"/>
            <end position="201"/>
        </location>
    </feature>
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        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH15191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="709" mass="82649" checksum="D8B756CDA911EA16" modified="2024-05-29" version="1">MSRLSEDEKIKVKEMTRAHVPPSQILISIKNENKENLTTMKQMYNYRQAIRKEDMEGRSVVQQLLSCLSKESYFYSYRASCTTDMVTDLFFSNKKAAQLLRQFYYVLIMDCTYKTNKYKMPLLQIVGCVPTGRNFVVGLTFLQDEKKDSFEWALRNARLLFDPNHLPEVIITDREYALIHAVDDVFSSSYHMLCTRHIAKNVEARARNETKNHLFALGVVGNRNRMVYAETEAEFEERLQHFKTTYVKRPNLLMYVENTWLFHVKKFVHAYTNKVLHFANRTTNRVESAHNALKRFLKTSTGSLDTIWTRVHAFLELQENEIKASFVRSANKDLHITVLHLLTQLTGKVSHLAIKKIMNEYNCHGVDSNPDTCGCYLRTSHGLPCAHEMRLLDEEGRGIKLNSVHVFWRTLHMEGSTYSSDDHHTQFEDDDNTLWSYFSIMMKQSSEVKKMYISQLEKMLHPKTNVLEEPSHDRKSKGRPTTRGSTKRDPSYFEHVEKKFPASKSGNKRKASTKEEPIPSEIPKFLLTYIHEYVIVSADGNCGYRSVAHQIYGSEDQWRRVRTDLYDFIERRLDFWARVWIKSIDEAWTTLHKINYCESSCPRQYWMSMDQMGPAIATMYNVILVVLDQIQIGTLTYLPLYAPEGSTNPEKLICVTFLRKFDHFVSVSLINNCPVLAIVGFWFKLHEDSVNGWDAPLKARVDEWNLIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWJ8</accession>
    <name>A0AAF0XWJ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannitol dehydrogenase</fullName>
            <ecNumber evidence="9">1.1.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NAD-dependent mannitol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0934224</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Oxidizes mannitol to mannose. Provides the initial step by which translocated mannitol is committed to central metabolism and, by regulating mannitol pool size, is important in regulating salt tolerance at the cellular level.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-mannitol + NAD(+) = D-mannose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15029"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16899"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.255"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.255" evidence="9"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWJ8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046029">
        <property type="term" value="F:mannitol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000004">
        <property type="entry name" value="probable cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000100">
        <property type="entry name" value="Putative cinnamyl alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="13">
        <location>
            <begin position="13"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056693"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH14702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="344" mass="36896" checksum="A29E94589C9EF95A" modified="2024-05-29" version="1">MKAFGWASREQSGTLSPFKFSRRATGIKDVTFKVLFCGICHTDLHMVKNEWGSSKYPLVPGHEIVGIVTKVGKKVQKCKVGDKVGVGCMVGSCRTCADCKNNLENYCPKMILTYGSTYLDGSTTYGGYSDTMVVDEHFVIRWPENLPLDCGAPLLCAGITTYSPLKHFGLSKPGIHIGVVGLGGLGHVAVKFAKAFGAKVTVISTSPSKQKEAIENLGADSFLVSRDAEEMKAAKGTMDGILDTVSAVHPLLPLLELLKSHGKLVLVGVPATPLEKIVAGSSIGGLKETQEMIDFAAKHKITAKVEVIPMDYVNTAMERLAKGDVNYRFVIDVGNTLKANSPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYU9</accession>
    <name>A0AAF0XYU9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0935081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Type-7 methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYU9"/>
    <dbReference type="KEGG" id="dcr:108201470"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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</entry>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <source>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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        <source>
            <tissue evidence="2">Leaf</tissue>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH16199.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="279" mass="32169" checksum="F50DD5DE6D7A139D" modified="2024-05-29" version="1">MELKQLEIRQPKLCFNPPLPRTPTSHLYTQCLYLFPNSNLYLFSPLRNVQAQYRRWDSNAESFRAQNFNFNPEPNKNDDEDDEAGQWLEVLEELIDGAWIIPVFRSFGWTLPAIISSLLITSGPKAFLMALAVPLGQSILSLAFQKIVGKKSKPKRKARTRRKPRTRPARNVRFEEEEEKEEEEAKEGSRNDSMGYQTWVGGNDVRDDSDSQSSPRFGGWDDLDRQSKSYKGSDKNFAQNTDRPQRSKLSRGRRKGETPLLLRLLIAVFPFLGSWTKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG81737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        </location>
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            <end position="142"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG81737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="25495" checksum="4670395A2761EDBB" modified="2024-05-29" version="1">MKGVLKGFKYISNIFEEEDEDEIQIGLPTDVKHVAHIGMDGPSTESPSWMKDFSGERPKSAPLDSKGAPIELTRTSRRRHSADSLVFDNNDNTVQPAKAKGSRKKKGTRRHKKDAASDQEGSSSKASEDTNTNGEPSPTSPTARKLPDIPKKSRRKKVKDEISKIATKAKPKASADSADGPPLSSPAPLPSPAPLPRPPATTKTTDSGPGSDSTTAVTCDGESCRITLKSPPNVAEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIR2</accession>
    <name>A0AAF1AIR2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000486">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000686">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000480">
        <property type="entry name" value="Pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053059">
        <property type="entry name" value="Inactive_SerThr-Kinase_ABA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48003:SF4">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48003">
        <property type="entry name" value="OS07G0626500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042210195" description="Protein kinase domain-containing protein" evidence="16">
        <location>
            <begin position="19"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="782"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG84899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1064" mass="115648" checksum="869137C900D532AC" modified="2024-05-29" version="1">MEIFRLLVVTCVLTCTMGQLPSQDILTLLEFKKGITHDPTGYVVDSWSEESIDFNGCPASWNGIVCNGDKVAGVVLNNLGLSANVDLSVFLNLTMLVKLSMSNNSISGKIPSNIGDFKGLVYMDISENLFFSSIPSEIGQLGSLQNLSLAGNNFSGSIPDSISGLNSIQSLDLSRNSFSGELPSSLTDLTNLVSLNLSLNGFEKKIPEGFDAMVNLKVFDLHGNSLEGNLDAEFLLLTTAVLVDLSDNLLVSSSSQRQKFLPGLSDSIEYLNLSCNQLTGSLIGGDQPQVFGNLRVLDLSNNDLSGELPGFDFVYELQVLKLSNNRFSGFIPNDLLKGDSSVLSELDLSGNNLTGPISMISATTLHTLNLSSNKLSGDLPLLTGNCAVLDLSNNQFEGNLTKLLKWGNIEYLDLSQNRLTGSIPEVTSQFLRLNYLNLSHNSLSSSLPKVITTFPKLTVLDLSYNQLDGRLIPTLLTFPTLQELHLENNKLGGSIEFSLPLSAEPSLHVLDISHNQLSGYFPDGFGSLTGLHDLNLAVNNFSGSLPTSISNISSLSSLDISQNHFTGPLPKNLPDSMEKFNATYNELSGVVPENLRKFPLSSFFPGNSDLQFPNPPPGSTPSESGQQNSRTIKTVVKVLVIIACVIAVILFILLAIFIHYIRISRRSRQQSVTSKDIRRHVQHNPSGLVGRVGGGGLVVSAEDDLMTSQKRPSSEIIVPDEKMAVITGFSPSKNSHFSWSSESGDSYTADLSRLDVRSPEILGGELYFLDDTISFLPEELSRAPAEVLGRSSHGTSYKATLDSGVFLTVKWLREGVAKQRKDFAKEAKKFSNIRHPNVVGLRGYYWGPTQHEKLILSDYISPGSLASFLFDRPERKGPPLTWAQRLKIAVDVARGLNYLHFDRAFPHGDLKATNVLLDGPDLNARVADYCLHRLMTQAGTIEQILDAGVLGYRAPELATSNKPLPNFKSDVYAFGVILLELLTGKCAGDVVSADDGGVDLTDWVRLKVTEGRGSDCFDAALAPELMNPASEKGSKEVLEIALRCILSVSERPGIKTIYEDLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJP8</accession>
    <name>A0AAF1AJP8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0102124</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJP8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000006">
        <property type="entry name" value="probable NAD kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000018">
        <property type="entry name" value="Probable NAD kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG82952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="524" mass="58048" checksum="E21DFECC4F0FA350" modified="2024-05-29" version="1">MAPSNFNSNNNASASSQENGFVDSCAPLNSEKAVQEIIKPLVQGTDDHLLEFSEVMRTVTKALRQVAEAKASAKAEAAEWKRKYEIERARYMNAENRVLSEEHISEPNGQRPENLANHVSLSKKAGDQSENICGNNGIFSHEVLLDGETERDSKVVQCKFKSKASFKLSWCCKGEKSDQHKHDIVSFDKGNITTAARSSKQISLKWESPPQTVLIMTKPNSASVRILCADMVRWLKEQKHLTVLVEPRVKSELLAESSFYNFVQTWTNETEILLLHMKVDLLVTLGGDGTVLWAASMFKGSVPPVVPFSLGTLGFMTPFYSENFRDCLASILHGPFNITLRHRLQCHVIRDATRNEDHSEDAILVLNEVTIDRGISSFLTNLECYCDNAFVTCVQGDGLILSTTSGSTAYSLAAGGSMVHPQVPGILFTPICPHSLSFRPLILPEHVTLRVFVPLNSRSNAWASFDGKDRKELAPGDALICSMASWPVPTACQVDSTNDFLHSIHDGLHWNVRKTQSFDSPRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKL7</accession>
    <name>A0AAF1AKL7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">DCAR_0103106</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="29">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="29">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKL7"/>
    <dbReference type="KEGG" id="dcr:108204738"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000463">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="20" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 26" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="21" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041908827" description="non-specific serine/threonine protein kinase" evidence="26">
        <location>
            <begin position="29"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="429"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="28">
        <location>
            <begin position="721"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00065357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="WOG83927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="854" mass="95989" checksum="803AFD6B16C651C4" modified="2024-05-29" version="1">MNHKHEQQLQQLLCCLLLLSILVFLGSATQLVSPGRRSLLSLPNDQTTTLVATLDGTIHLVDPKSEKVLWSQATGAPIYTSYHTPVDHNESVPGPLYYVDCGDDWMLYAHTELGKMKLDRSIREFVVNTPHVSEDGGILNGAMKTTVFVLDANSGRRIHTCGPNESPELHRTGSIHMKADELPLHITRTDYLLSYASKKYTWNVSVSEIGAAFLCQDIENSISSSRANSGNELPSKPAVQINMPLACQSKAVVARLRSFNVLEYFSKQDQRPLGYHEDMMLPAPSPDYMPLSQAKAGMSLDAHPDSGFMFALPELSNNFEISSPDVKKSYRGVLSLVPFLFIGMGALIYFCAILPGHPARNVSANNASSRKKKNRKSTKYGGIVEKKDKKTLFDLEDGNTTSENDRKSFLNFNQLSNQNADGRVVGKLFLMNKEIAKGSNGTVVLEGIYEGRPVAVKRLVRAHHDVAFKEIQNLIASDRHSNIVRWYGVEYDQDFVYLSLELCTCSLNDLILMHSDHSRKSVYSGARASRAMTEYKVRLDSMKGILQDIELWKANSYPSTMLVKLMRDIVSGLAHLHELGIVHRDLKPQNVLIVQDKIPSAKLSDMGISKRLAGDMSSLGHATGNGSSGWQAPEQLLHGRQTRSVDIFSLGCVLFFCITGGKHPFGDPLERDINITKNKVDLFLVQHIPEAVDLLSCLLDRQPGLRPTALEVLHHPLFWNPEMKLSFLRDTSDRVELEDRETASDILIELESVAAMALGAKWDEKMEPAFLANIGRYRRYKFDSVRDLLRVMRNKSNHYRELPKEIQELLGPVPEGFYNYFGDRFPKLLIEVYRVMYKWCKDDEWFSKYSSSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKZ4</accession>
    <name>A0AAF1AKZ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Frigida family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AKZ4"/>
    <dbReference type="KEGG" id="dcr:108223051"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012474">
        <property type="entry name" value="Frigida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791:SF47">
        <property type="entry name" value="INACTIVE FRIGIDA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4 5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="563"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="180"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="653" mass="76000" checksum="0A8B25325FAEA3DE" modified="2024-05-29" version="1">MEVSESRRARVEKAMHSLLVEWKNLEHADSSTQRWVQDCCADIQSRQERLNTVQESVTSSHRKLEEITKSVGERLEEVESRERGLKAMHEELESKMKELDSVKGDLESGLIDVEAREKRIEDQMKLIEGVFEKFCSERKEVERIKGWLEGRFEEVKLKEKGLAIKEKSIKNREIKLGKLMADYDLKMKNVEAKEKELEIKEKSIDDHKSKLDQVLHHYMGEMMNVMKKKEEVKNREDELDEMLVEIDLSKRNVIEMEKQVEFKEKIIQIHETNMNKAWAELEMKSKEASSVEYSIVKPEPWSDDGSYADIRFSVTMDGNSLLLYLINHKGDLDSMSDEVYEALGKSKEPGKLVLDALQGFYSKKEAEEFEEDVVCRSSSLLLEQLRRISPHIQSYHKRAALKLASQWKEKMKSSKEFIVFLQLLASYRLESSFHPEEFFSLFEVINQPTEISELFQLLNYMGKVNDLITSLIEKGRRLMAIRYIYGFKLVEKFPPVPILNDHISFSNLLAEKIQVQKSSDEPEGIAIGKQLASLRAVVKCIKDHQLEVEYPPESLLARIQQLKAKRADMPQNSGKKRRAAGPKAQTGGSNRKRRHEIWDEMPYEDKATIMFMQPDHYRVEAERMTAIHKQEGILKFAGMMAHKLKKHKEEDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANS1</accession>
    <name>A0AAF1ANS1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock 70 kDa protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0209210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 (TC 1.A.33) family. HSP110/SSE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANS1"/>
    <dbReference type="KEGG" id="dcr:108208819"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24095">
        <property type="entry name" value="ASKHA_NBD_HSP70_AtHsp70-14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF10">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 16 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="498"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="762"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG89969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="86756" checksum="37718718DE5E6A06" modified="2024-05-29" version="1">MSIVGFDMGNENCVIGVAKQGGIDVLLNEESNREIPAVVSFGEKQRFIGLAGAASATMNPKSTISQVKRMIGVKFREMEEDLRLLPFETSEGPDGEILIHVQYLNEKHSFTPVQILAMLFAHLKQMSEKSLMTSISKCVIGIPSYFTDQQRRAYLDAAEIAGLHPLRLMHDCTAIALGYGIYRTDFPSKRRSNVVFVDIGHCDTQVTVAAFRPGHMEILSHSFDRNLGGRNFDEVLFKHFATEFKEHYNIDVYSNTRAAVRLRVACEKLKKVLSANPEAPLNIECLMDEKDVKGFIKREDFEKLSADLFERISHPCQKALLDSGLSMDKINSVELIGSGSRIPAVKKILASLFGTEPRRTLNASECVARGCAIQCAMLSPIYRVKDYEVKDSFPFSIGLSLDEGLINNSNGILFPKGCLFPSEKILQLHRRSTFHMEAFYADQNELPSGISPQMSKFIIGPLQVSDAEKVKVKVKVLLNLHGIVMIQSATSLIEGCRDNSSGKSNAHSTSEHIEGDNHVSSDVANGASAESYLPTKRTADDMREEEIQRQDIPVSESVCGGMTWAELSEAQTKELQLTQQDIKMERTQEKKNLLESYVYETRTKLFNTYRGYAAESEREGISSNLQQTEDWLYEDGDDESEIVYSEKLAHLKELVDPIERRFRDEEARVRAASRLFNCIEDYQKAVGSLSSDQKEAVLIECDKAQQWLLEKIQQQECMPKNADPVLWSSEINKKAETLDATCFHITRPKDSATKPDEVMDDRNEEDDNMSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQE3</accession>
    <name>A0AAF1AQE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="7">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0310899</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQE3"/>
    <dbReference type="KEGG" id="dcr:108210391"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="9">
        <location>
            <begin position="91"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG91649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="426" mass="45299" checksum="7016D4FA3598E4C2" modified="2024-05-29" version="1">MATSSLFRSTVSPAGCGGASRSELRPSGVPKVSSVSFCRGSLKSESSIFGASIPGNSSSLHKSSSRSIQTIQATATELPPTIQMSKSGGKTKIGINGFGRIGRLVLRIATFRDDIDVVAVNDPFVDAKYMAYMFKYDSTHGVYTESIKVLDDSTLEINGKKIKVTSKRNPAEIPWGEYGADFVVESSGIFTTIEKASEHIKGGAKKVVISAPSADAPMFVVGVNEETYKPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATTATQKTVDGPSMKDWRGGRGAGQNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTANVSVVDLTCRLEKSASYEDVKAAIKYASEGPLHGILGYTDEDVVSNDFVGDSRSSIFDAQAGMGLTSSFMKLVSWYDNEWGYSNRVLDLIEHMALVAATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AR20</accession>
    <name>A0AAF1AR20_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0209031</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AR20"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042014781" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="24"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="334"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG89792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="636" mass="71699" checksum="584338E6069D0138" modified="2024-05-29" version="1">MGVRVYLHLSWIIVIVAVRVVVGENVSAAGVQECRMTRCSHHGPEIRFPFWIKEKQQPEQCGYPGFRVFCDRGNTLLHLQYLANTSLPDTLFYLSKNVSIHSINYTSQEVFVYVLNGSQFTNNLKLFSASSTSLPSTPHFGKAYSYDFANMYYPDITYTMCFSCSAGVKSFIPDMLTSPGRKTFPVYCLDDQYSAFSGEYSISSCTKIFNSSLPAPLLSQGYDPYAPPGVVDALSISWLAPNCSKCEAKGEYCRRKSNASSNIEAADYSTICFPKVITAGSAIFVLSIMLLLYYIIKLYKQRKYDEQKIEMFLTDYRAMKPTRYSYADIKKITRNFSDNLGKGGYGSVYKGQITKEIIVAVKVLNSDPKANGEDFINEVGTIGRIYHVNVVRLVGYSADGCNRALIYEFQPNNSLEKFTYSGKMLHDFLGWKKMQDIALGIAKGIEYLHQGCAQQILHFDIKPHNILLDQNFNPKISDFGLAKLCSKDKSIVSMTMARGTIGYIAPEVFSRNFGKVSSKSDVYSFGMLLLEMVGARNNNEVESTSETYFPEWIFHRLEEGGEVTIQIVKEEDSNIARRLTIVGLWCIGWHPVDRPSMKHVINMLESEECPKVPPNPFRSSNVRSFTNDLEVISESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AS75</accession>
    <name>A0AAF1AS75_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0209619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="44"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="119"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="347" mass="39905" checksum="839AB63D0F71D869" modified="2024-05-29" version="1">MEALSDFFTTQFSKFQVDFRGEHKDTAITSTGPNDPHIIEEHWSHPDHPLELFQFIINEHDSDDEDDDDNTTVLICDGCTQHITVTHPSYYGCTQCNFFLHSVCAIKLPKVLPIGISSFHTQHPLALTRKPRFYTTVDCAICGWETNGFYYNCEACDMKIDICCAFLPSRIKYKSHKHHPFILRPSWGEACSVSREEITGGMEYGCEICSNFRMNVSSVLVPSTMKHKYDASITLRYPPFFYEGAFYCELCEDRVNNQRWLYHCDKSDHSFHSDCLFSWQKIKLGGTVRIDINDKMHTLAFVFKMNVSAALYICGNCGDGYTHSLFFERDGCGYLICVNCVRDRYGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AT25</accession>
    <name>A0AAF1AT25_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0310553</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">May be involved in membrane trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000100">
        <property type="entry name" value="Extended synaptotagmin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000135">
        <property type="entry name" value="Plant synaptotagmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
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    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF149">
        <property type="entry name" value="SYNAPTOTAGMIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="60"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="237"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="418"/>
            <end position="535"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG91305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="560" mass="62743" checksum="3CE1DF415D3FE45A" modified="2024-05-29" version="1">MSFVVGLVIGLVVGLGLIVAFVRSENARSKSRSQLAETVAAFARMTVEDSRKLLAPEYYPSWVLTWLNEHLTKIWPYVNEAASELIKTNVEPILEQYRPIILSSMKFSKLTLGTVAPQFTGISIIEDGCKDGITMELEMQWDGNPSIILDIKTRVGVGLPVQVKNIAFTGVFRLIFKPLVDELPCFGAVCFSLRHKKKLDLTLKVVGGDISAIPGISDAIETTIRDAVEDSITWPVRIVIPIIPGDYSDLELKPVGRLEVKLVQAKELTNKDIIGKSDPYAVLYVRPIRSRTKTSKTISNQLNPVWNEHFEFIVEDASTQHLVVKIFDDEGLQASELIGCAQIRLSEIEPGKVKDVWLKLVKDLDLQRDNKNRGQVHLELLYCPFGMKNGFANPFSPELSMTSLEKVFKSGEEDMDYAENGGEVKNKRREVITRGVLAVTVISAEDLAPADVMGKADPYVVVSMKKTAAKNKTRVSNDNLNPVWNQTFDFVVEDGLHDMLILEVWDHDTFGKDYMGRCILTLTRVILEGEYKDSFQLDGAKSGKLNLHLKWMPQPIYRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATD9</accession>
    <name>A0AAF1ATD9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATD9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006315">
        <property type="term" value="P:homing of group II introns"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090615">
        <property type="term" value="P:mitochondrial mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33642">
        <property type="entry name" value="COX1/OXI3 INTRON 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33642:SF5">
        <property type="entry name" value="MATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8903" checksum="3D5DCAC504CC01AD" modified="2024-05-29" version="1">MKEAIRMVLESIYDPEFPDTSHFRSGQGCHSALRRIKEEWGTSRYVLLSALLGNIYLHKLNQEIGRIRQKYEIPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVI4</accession>
    <name>A0AAF1AVI4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0313052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVI4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000091">
        <property type="entry name" value="Gastric triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="3">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="619" mass="69885" checksum="BD0F73AC0D626D50" modified="2024-05-29" version="1">MHYWKLRPTGVSSFNQFIHELSVDSDMGSSVDNSSGEDYSDDGATPLTPRSESSQASSELSFSKHDMCQAKLALCFRYILAWILFPAKFLAGILTYFYIANTTLGNLQHLYSESKTKMLKDHSVQHAIDRRRGVIEDVHLLTEIFIEVVFDFIHKAACYFLSPLETSEMLISWFSSSSIKHIPDDASEVLVQTATLADTDPALKERTVVPLYSLNTDGRTCREVITELGYPYEAISVVTSDGYVLLLERIPRRDSQKVVYPQHGLFDSSIGWISNGVVGSPAFAAFDQGYDVFLGNLRGLVSREHINKYISSRKYWRYSMNEYGVEDIPAMIEKIHEVKTTELKYCELKAEEEQTSGDQPYKMCAICHSLGGAAGLMYAVVRRIEEKPHRLSRLILLSPAGFHDDSILTFKILGNIFRWSAPVLEPLVPGLYIPSWFLRMLLNKFARDLHNYPAVGGLVQTLMSFVAGGDSSNWVGILSLPHYNMYDMPGVSFNVALHLAQTLHSKKFQMFDYGSAAANMEVYGSSEPLDLAEYYELIDIPVDLVAGRKDQVIRSSMIKKHYTRMKNAGVNVSFQEFEYGHLDFTKAHSDEILAYVISRLLLVAPRPGQKALRSKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW53</accession>
    <name>A0AAF1AW53_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0313736</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW53"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000423">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000086">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041967471" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="341"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOG94440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="691" mass="76670" checksum="81062F11123B9F78" modified="2024-05-29" version="1">MPKKITLLSSFIFLTIQAFCLDDFIFNGFKKPETNLSLSGTARIDKHGILQLTNNTGRLMGHALFPSPIQFKNSEDGPVKSFSTCFVFNIVPEYPRLGGHGLAFTVAPSQDFIKSALPSQYLGLLNATEVGNFSNHLFAVEFDTVQDFEFGDINDNHVGININSLVSNKSAKAAYFSDDLVQQDLNLKSGKTIVAWIEYDSIRNLVNVTLSPSFSKPRLPILSYKLDLSPIFQKSMYIGFSSSTGLLASSHYVLGWSFKTNGDAKVLNFKSLPSLSGHKKRSPVFKILVSVLSVLVVLGSVFLVLYALRKYKNRDIIEEWELEVGPHRFSYQELKKATKGFKESELLGAGGFGKVYKGILPSSKLEVAVKRISNESKQGLREFVSEITTIGKLRHRNLVQLQGWCRLRGDLLLVYDYMPNASLDRFLFEKPIAVLSWEQRFNIIKGVASGLFYLHEGYEQIVIHRDIKASNVLLDGDLNGRVGDFGMAKLYEHGENPTTTRVVGTLGYLAPELPRTGRATTSSDVFAFGTLLLEVVCGRRPIENRAPPEEFVLVDWVWDKWIQGKPFDVVDKKLEGEFDENEVVVVLKLGLMCSNHVAKSRPSMRQVVRYLEGEVGLPGMVRPPGGCYGDGKGGGEGFDDYINSFASSSFDKGSLYSEGAQADGDLSFVSVSASPMSLLLPPSLFMCPLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3D1</accession>
    <name>A0AAF1B3D1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EMB1273</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3D1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36000">
        <property type="entry name" value="DEFECTIVE 1273 PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36000:SF2">
        <property type="entry name" value="DEFECTIVE 1273 PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23269" checksum="9DD433DF999456A0" modified="2024-05-29" version="1">MLTVNTNNGYRNSRKKTICAINMTAGGLGEPMKAKLDHISQKARSIWDSSPQPVKSFPWNKAAESFIQLIIDLVLAVMKYLSIPVLAISSLSEMSYCAHERKLFLIPIPILVGVALAGILKETALELSPLLKQAEVPWHLVAVAIFFTLIKLPGPYYPYWGRILIPHIANGGLLRTLWFAFLWYRRPKKAAANIPESVVDNFPEQNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3L7</accession>
    <name>A0AAF1B3L7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0624352</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3L7"/>
    <dbReference type="KEGG" id="dcr:108225226"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.110.10:FF:000001">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042285808" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH04940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="171" mass="17367" checksum="1C7EFF46450AF2C8" modified="2024-05-29" version="1">MRTHLYILWTLLILGVAHVSRASHHAAPTPAVDCSTVVLNMVDCLSFVTSGSTVSKPEGSCCSGLKSVLKSNAECLCEAFKNSAQFGVSLNVTKAMTLPAVCKISTPVNNCGTLSPMALPPSSISDAPAMTISTDDGTPAPSPGSSGASSALVVSASLLVLSLIVASVSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3P2</accession>
    <name>A0AAF1B3P2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="9">Transmembrane protein 50A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0622886</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3P2"/>
    <dbReference type="KEGG" id="dcr:108225021"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14781" checksum="80C84F4F3DBD8477" modified="2024-05-29" version="1">MDVGELWAIFGPGLAGAVFGAGWWFWVDAVVCSAVAVSFLHYLPGIFASLAALMFNCVRREDIDYSPYDDGEWRLKLWLFIAYVVAFVSLAASVGLLIQDALEESGPSSWTGTAGVFQCVFVLISGLIYWTSHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4F7</accession>
    <name>A0AAF1B4F7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4F7"/>
    <dbReference type="KEGG" id="dcr:108226876"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006927">
        <property type="entry name" value="DUF639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860:SF5">
        <property type="entry name" value="ARGH (DUF639)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04842">
        <property type="entry name" value="DUF639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="613"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="684" mass="77015" checksum="A67AEA8DDDC2A2F6" modified="2024-05-29" version="1">MTTTKTAERKHLSPIANDVITRCCQKMDTSVDALVKEFEAIWKPENGDYSRKMVEYCSSKALNEMCSNIKELISDGNFSRLSFDMMLAWEKPSSVAQYQESLSECVAKEDEDAVSARLNHQQDQIPLFYSDIMPLLVDREPSVGEDAFLWLVTVVPLLADVVNGRFTFETLTATTVNRLHFPAYDKFLKESDRCIKHLQKQEIPSGVELVDDEFILHVEGTASTSRVVRHIGVTSWPGKLTLTNYALYFEASGVISYEDAIKLDLSKGIGQSVKRAATGPWGAPLYDKAIIYESSELRESFVLEFPEMASSTRRDHWLALVKEVILLHQYILKSKVESPVQVWEMHARTILGIIRLHAAREMLRISPPNPKSFLIFTLFEELPKGDIVLETLVESLKNVSSGHPCSASSILRNLNVTQACPLCTDTKDIIEAPEVLSGQAENMSSLESVIEQIRDEAREVNIAKATTETIKEEGLSDSYAVLMELLNSLRSAILPWSQDVLTWKKPGITVMVAGVTLLIVYMEWIFRAIAALLLWTVSKMIWARRARVADKYTKLVVFVDSEQTTVGSLVSAQHGMNTINELMQSANISILKMWSILISKAPKHTNMVMTSMTTLAVALAVIPFKFIIMSFLLYIFTTTSKLRKNTKSQPGNRRLKEWWDSIPVIPVEIVEQQDGSSKSVCYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B535</accession>
    <name>A0AAF1B535_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0622915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B535"/>
    <dbReference type="KEGG" id="dcr:108225657"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="365"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH03516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="455" mass="52082" checksum="41B0755266F4C498" modified="2024-05-29" version="1">MEATDHDEEFLNLTLAIDSNSSFDKKRKGMKTEEVLIEPKEECEGKILNLLQLREDMLKLDHKRKGVAEDGKGLHLIHLLLISATSVDENKLDSSVESLSELFQNVCLSGDSIQRVAAYFADGLVARLLTRKSPFYDMIMKEPTAEEEFLAFTELYKVSPYYQFAHFTANQAIIEAFEKEEGYNNNALHVIDFDVSYGFQWPSLIQSLSDKTTNSHRISLRITAFGRSTEELHETETRLVCFAKGFRNLVFEFQGLLRGSSFSNIKRRNNETLAVNLVFHLNTLTSFSKISDTLKSVHFLNPATVILVEQDGSRNPRSFLARFMESLHYFAAMFDSLDDCLPLDSAERLGIEKYHLGREIKRFINFEKDVTNCMKYERMETWKGRMEGHGFEGVKLSSKSVMQAKLLLKIRSQYCPVQCDGENGGFRVFERDENTAISLGWQDRCLLTASAWHCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B972</accession>
    <name>A0AAF1B972_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0830372</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Macko-Podgorni A."/>
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    <dbReference type="Gramene" id="KZM93300">
        <property type="protein sequence ID" value="KZM93300"/>
        <property type="gene ID" value="DCAR_016545"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223718"/>
    <dbReference type="OMA" id="CEMAPLE"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056993">
        <property type="entry name" value="TRIP4_3rd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23134">
        <property type="entry name" value="TRIP4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="2">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Activating signal cointegrator 1 third" evidence="3">
        <location>
            <begin position="250"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM93300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG99558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="380" mass="41551" checksum="2AA0A19CE5C3B3B2" modified="2016-07-06" version="1">MGSSGEWLEKALLELCNKKTGSGLGLELDSDLISGLVSYCECASPLDAKEYLDNIIGEEAGKGVTEEYLKQRGHSDIDNTTLDGSGLKLHAYVKPPSNESSSSGVKKAPKIQREAAISSKKDYAVQSERVEPKAAEKGNSKKKKSGKVISLAEAAKGTIVYQQGKPCSCQARRHRLISNCLSCGKIVCEQEGEGPCNFCGALVLREGSSYAGLEVSEIPLSDTEVAAQAYAKRLVDYDRNSAARTTVIDDQSDYYNIEGNSWISPEEKELLRKKQEEIEEDERAKRSKVVMTFDLVGRKVLMNEDEISEEFQNSLLLRPADEREANRIKPNPSLTVQPVFVDPGPSKKSTKGSLRTGIANGLCLEISGRIQHDSKELSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A7J8</accession>
    <name>A0A162A7J8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_016048</name>
        <name evidence="4" type="ORF">DCAR_0414646</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A7J8"/>
    <dbReference type="Gramene" id="KZM96590">
        <property type="protein sequence ID" value="KZM96590"/>
        <property type="gene ID" value="DCAR_016048"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216593"/>
    <dbReference type="OMA" id="WSFIELH"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000351">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000682">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g16610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000436">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39350 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000475">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g40410, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF451">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="426"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM96590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="86372" checksum="13CF52B93F03FED3" modified="2016-07-06" version="1">MKKLKQALSNPRKPQFSPQPHLTTHFCNSIINRHSSKGAHASVLHAYISLFQPNNISPDAYTYPSLLKSCTFFNLFPHGVLFHQQIIVNGFSSDAYVASSLINFYAKFGYTNYARYMFDGMSNKNVVPWTAMIGCYSRIGDVGTAFSMYKRMVWEGVRPSSVTMLALLSGVCEDVLSVECLCGCVVKLGFDCHVAVVNSMVSLFGKYGRVEDARKLFESMGGKDIVSWNSLVSGYALTGNVREVMFLINRMRFEGLEPDHQTFGALVSATVRQNNVNTGKLVHAHILSSDFQLDLPVKTLLISMYLKFGNHDYAYTIFKQTLDKDTMLWTTMISGLVQNDFADRAVVLFREMLSSGVMPSSVTVACALAACAHIGALDLGTSIHGYMVRQHMSVEVATQNSLVTMYSKCGHLERSCKVFDMMDKKDIVSWNAIVSAHAQNGDLCKGMCLLKEMRLASEIPDSITVVSLLQACASIGAYHQGKWIHSFIIRNAIESCILVDTTLVDMYSKCGNLESAKRCFDRMLKHDAVSWSTIISGYGSNGKGETALELYAEFLRTGLRPNHVIFLSILSACSHSGLVDYGIRLFHTMTKDFQLEPKVEHCACIVDLLCRARRVEDAHDFYKKMFAEPVVDVLGILLDACRSNGKAELGETIAEEILMLQPGDAGKYMQLAHNYASTARWDDVGKALIQMRSQGLKKLPGWSFFESQGTITTFFTDHSSHPQYEEIVAILMFLSKEIRKPDIMTPEKEKIDGDYRNFENTSFLMQELLHVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AS16</accession>
    <name>A0A162AS16_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0206625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AS16"/>
    <dbReference type="Gramene" id="KZN05053">
        <property type="protein sequence ID" value="KZN05053"/>
        <property type="gene ID" value="DCAR_005890"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209322"/>
    <dbReference type="OMA" id="EDDCMFP"/>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG87401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="492" mass="57156" checksum="FFD7D95366E974C7" modified="2016-07-06" version="1">MGLLSKSKPRSLFLYPRYVYPFAAFLSLSLFLLFQVHNYVSRTKTVAGHNLEPTPWQIFPDTEKSNFETGFTKASKVIQCSYFTCGSGNNVPVQSKLSMSSKYGENCPEFYRWIHHDLEPWHKSRISLAHLMAAKETAAAFRVVIIDGRLYVDSFYGCVQSRALFTVWGFVQLLRRYPGQIRDVDLMFDCMDKPIINRTEHALWPFPLFRYCTSAQHFDIPFPDWSFWGWEEVNVSPWDEEFKSIKKGANSKSWKKKIPYAYWKGNPDVVSPIRLALLECNDTELWGARIMRQNWTEEAEHGFSKSKLSNQCKNRYKIYAEGYAWSVSLKYILACGSVPLIIHPEYEDFFSRGLFPKKNYWPISPTNLCPAIKYAVEYGNKHARQAEAMGKAAQNLMESISMDGVYDYMYHLLNEYSKLLDFKPVRPSTAIEVCYQSLLCYADEKKRKFLERSTAFPSLNPPCKLPPRDDNYIKSWMEKKSKAIEEAEIIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AUV9</accession>
    <name>A0A162AUV9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0207984</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AUV9"/>
    <dbReference type="Gramene" id="KZN06233">
        <property type="protein sequence ID" value="KZN06233"/>
        <property type="gene ID" value="DCAR_007070"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209560"/>
    <dbReference type="OMA" id="TKPYYPP"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF208">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG88749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="608" mass="67844" checksum="8BB9458BC88DDC11" modified="2016-07-06" version="1">MDLKKVGFPDHFASVKRKVVSGFGLGLVVSLIFLSVFVFNSSFKTPFFNPIFQGLNTFGSNSSSVTLSWPFSSSSAYSKIVNDTAVTHANGTVAKLPDSLKTPVANVSQVGLNGTFVSDSGKGGDLEGAHVGNSSDLVKDETFGGDGNVIVEKSIEGKVSGSLVGESVGLGDDLSSGNGSSSVSGGESVGSGEGLSSSNGTNLISGVGEISNLTHNGENGASLSSPEVMNPVTTSEKVNATISSNRSQSVEVKKTSLDKCDIFDGRWVRDDTKPYYPAGSCPHIDRDFDCYLNKRPDSEFVKWRWQPNGCDIPSLNATDFLERIRGKKLVFVGDSLNRNMWESLVCILRHSVKNKKRVYEISGKKEFKKKGFYAFRFEDYDCTVDFVSSPFLVRESYFKEKNVSLETLRLDLMDRTTSMYRDADYLIFNTGHWWTHEKTSKGENYYQEGNVVHTRLKVLDAYTRALNTWARWVDKNIDKERTQVFFRGYSVTHFRGGYWNTGGQCHQETEPIYEDVHLIKYPSKMRALEYVIQDMKTPVVYLNISKLTDYRKDAHPSIYRMEYKTQEEQIAAVHSQDCSHWCLPGVPDTWNELLYASLLKIGKGSWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164T7E8</accession>
    <name>A0A164T7E8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_024292</name>
        <name evidence="18" type="ORF">DCAR_0728076</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164T7E8"/>
    <dbReference type="STRING" id="79200.A0A164T7E8"/>
    <dbReference type="Gramene" id="KZM87158">
        <property type="protein sequence ID" value="KZM87158"/>
        <property type="gene ID" value="DCAR_024292"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196784"/>
    <dbReference type="OMA" id="EYTNLIY"/>
    <dbReference type="OrthoDB" id="1926347at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046739">
        <property type="term" value="P:transport of virus in multicellular host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000001">
        <property type="entry name" value="cysteine-rich repeat secretory protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000008">
        <property type="entry name" value="cysteine-rich repeat secretory protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051378">
        <property type="entry name" value="Cell2Cell_Antifungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080">
        <property type="entry name" value="ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080:SF24">
        <property type="entry name" value="PLASMODESMATA-LOCATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853282" description="Gnk2-homologous domain-containing protein" evidence="15">
        <location>
            <begin position="28"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="142"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KZM87158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH08632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="305" mass="32694" checksum="44D0224F6DB5A910" modified="2016-07-06" version="1">MGLPTTPFHIFSLFLVIFANLELLAEAKSAPDYNTLVYKGCAKQSLSDPTGIYSQAISTLFGTLVSQSSKTRFFKTTTGSSQTTITGLFQCRGDLSNVDCYNCVSRLPILTDKLCGKPVAARIQLLGCYMMYQVAGFAQISGLELLYKTCGRTNVGGSGFEERRDTAFSTLESGVTSGNGFYTTSYESMFVLGQCQGDLGSSDCASCVKTAVQRAQVECGSSSSGQIYLHMCFITYAYYPNGVPKMSSPSSSSSSSLYNPSSSSSDRQQDTGKTVAIILGGAAGVGFLIICMLFARSLLKKHDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U0U2</accession>
    <name>A0A164U0U2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0729108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U0U2"/>
    <dbReference type="Gramene" id="KZM88281">
        <property type="protein sequence ID" value="KZM88281"/>
        <property type="gene ID" value="DCAR_025356"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196871"/>
    <dbReference type="OMA" id="DKGGDGF"/>
    <dbReference type="OrthoDB" id="685187at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="229" mass="25537" checksum="911A0FD5803E6A0F" modified="2016-07-06" version="1">MQTPSTDYHSNVDTSRPFSSVKEAVAVFGERFLTPAVYSPKPFSFPKQESSNSSSWNFFTPTPSPTYSHASKQTAWKDRDSEATLAETIKKLESELEETKEEVKLLKERETENEVALASLNAELHKNMSKIAQAEAMAARKAAESGGEGEGQSRRDGIVKMGSSPSLSQILSFSQEEKEELFGGRKKERKSMRKKKPIIPLVGDLFWRKKGSSTSVLNPIYSSPSVHWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VFL5</accession>
    <name>A0A164VFL5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_022361</name>
        <name evidence="3" type="ORF">DCAR_0623817</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VFL5"/>
    <dbReference type="Gramene" id="KZM90274">
        <property type="protein sequence ID" value="KZM90274"/>
        <property type="gene ID" value="DCAR_022361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853895" evidence="1">
        <location>
            <begin position="26"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM90274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH04408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="78" mass="8698" checksum="5732BFC9AAAEF7D1" modified="2016-07-06" version="1">MASVKLAGLLLCLLLVFHASRDSSALVDLDKYASQESVAGHRHQDQDKDLEATKQEVMNLMYKDYDPPRAASSQKPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VK39</accession>
    <name>A0A164VK39_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_022169</name>
        <name evidence="9" type="ORF">DCAR_0623985</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VK39"/>
    <dbReference type="STRING" id="79200.A0A164VK39"/>
    <dbReference type="Gramene" id="KZM90466">
        <property type="protein sequence ID" value="KZM90466"/>
        <property type="gene ID" value="DCAR_022169"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225277"/>
    <dbReference type="OMA" id="NKTFHEG"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000003">
        <property type="entry name" value="Trafficking particle complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM90466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH04575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="140" mass="15823" checksum="FD456804388D9142" modified="2016-07-06" version="1">MPAVYSLYIINKSGGLIYNKDYGSAGRMDTNDSLRIASLWHSMHAISQQLSPVAGCLGIELLEADTFELHCFQSLTGTKFFVVAEPGTQNMESLLKHIYKQYTDFVLKNPFYEMEMPIRCELFDVNLSQAVQKDRVAFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VWL3</accession>
    <name>A0A164VWL3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0624547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VWL3"/>
    <dbReference type="Gramene" id="KZM90967">
        <property type="protein sequence ID" value="KZM90967"/>
        <property type="gene ID" value="DCAR_021668"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226438"/>
    <dbReference type="OMA" id="RASAYCD"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd22140">
        <property type="entry name" value="F-box_D3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF746">
        <property type="entry name" value="OS01G0185400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="491" mass="54081" checksum="A9863DC41DB0DB45" modified="2016-07-06" version="1">MAGMASGTKITDMGMDSLIHCASFLNLQDISNLSLTCKYLNSLAYSDSIWRSFFRQRWPQQEPSSISERSSVREAYITRNFDVQQFKFVDPFAADILTDAKPFDNLFINKNDIVFSQGPAINIMKIDSFLKRREPLLTLRDHSARVSCMRFYSLDETSLFRSETQRNEKVLVTSSYDHSIRLWWKGNCQRCFRGHSGPVTTLSDKLLGEGSGKVFASGGDDGTVRLWSLNLNGKRGQHALRATLYGHGKPLVLMSVAGHKSSLLVSMSKDSKLRLWDTATSSSSSSCCVGMTSIPGAPVGLKCHESLLFVAAGSSVVAIDLRTMKKVFSTPYYPPSLFSFEILPSKSLICTGGMNSAMLWDMRTFSETVKTKPLGLLDGHIGPVKFLHMDRYKVVSGSPEDPFVNVWESDTGRQTNILACNPTGGLHSTTGGSAIAVNGCEIVTGSCTEGQGIIHYRNFNYATSQVLAEENEGGSKFWGPQHCSDSYESDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VYN5</accession>
    <name>A0A164VYN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-disulfide reductase</fullName>
            <ecNumber evidence="1">1.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_021589</name>
        <name evidence="9" type="ORF">DCAR_0624651</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VYN5"/>
    <dbReference type="Gramene" id="KZM91046">
        <property type="protein sequence ID" value="KZM91046"/>
        <property type="gene ID" value="DCAR_021589"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047134">
        <property type="term" value="F:protein-disulfide reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052259">
        <property type="entry name" value="Nucleoredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13871:SF96">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="7">
        <location>
            <begin position="107"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM91046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH05237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="407" mass="47084" checksum="DC40EA953D0A00C1" modified="2016-07-06" version="1">MRIDDGCGALQKLAKLRFPLSPWIGESDTASVVVDPTGVVLKWFADDYFVRYGPEAYPFTDKRIGFVKSRDKEALKHPSITNLLTSPQRDYVINNKNPEIPVHTLQDKVVALYFCQEYPLDPLTKGLKMTYKQLADKKFEIMLVYIHNSFITSARASEESFWKSFNKMPWLALPFKDQKCIDLQRVFNLPLDALGPGPDPSLVIIGPQGKFVEPYGVDIAQNFSISAYPFSRKRVAELEVNYIKELKLDMFWDRNTSFIQKDGTEIKLSQLVGKRIMLVAQNDWYYGINAKFWRMLEARYSKMKGTSKEFEVIHIRKKQVSSNGKHRATMPWLWHPPLLEGSCQLELLLRLLRQGVGLLAFDGDGRVVRRTKFPRIEMNNVDFPFYAGGLEKEAVTELIERFEWYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XU49</accession>
    <name>A0A164XU49_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_016831</name>
        <name evidence="15" type="ORF">DCAR_0519244</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein functions as a defense against chitin containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XU49"/>
    <dbReference type="Gramene" id="KZM93586">
        <property type="protein sequence ID" value="KZM93586"/>
        <property type="gene ID" value="DCAR_016831"/>
    </dbReference>
    <dbReference type="OMA" id="AWVGNAM"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Acidic endochitinase SE2"/>
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        <property type="entry name" value="Glycosidases"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
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        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
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        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KZM93586.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG99888.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="31629" checksum="78CB037D4C220389" modified="2016-07-06" version="1">MASKLAVSLAMFSSLVLLLVVGSDAGSISIYWGQNGQEGTLADTCATGNYEYVNLAFLATFGNGQTPMINLAGHCDPYSNGCTSLSSDIKSCQAQGIKVILSIGGASGSYSLVSAADAREVATYLWNNFLGGQSATRPLGNAVLDGVDFDIEGGSGEYWDDLARYLSAYSKRGKKVYLTAAPQCPYPDAWVGGALQTGLFDYVWVQFFNNPPCQYSSTATSNLEDAWKQWTTDIPATKIFLGLPAAPDAAGSGFIPVNDLTSQVLPAIKGSDKYGGVMLWSKYYDDQSGYSSSIKSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Y526</accession>
    <name>A0A164Y526_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM93988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="211" mass="24177" checksum="22C939D0E52EC6CF" modified="2016-07-06" version="1">MNKYSYTLLECTIKLYWDYQQKIEISKFLEVPKVKAPGPDRLSSVQYEYEELQHMVHALNNENHFLKEELQKVSEECNKGSVFAALLEEDSLAYSDSFICGSGLLDNTQNVKPSTDDIPKEVVPLSVSCWAEDPADGPEFIEIRYFLENFIHCLCTPDMSPPRMIKMGHANEVSATVEYPCTSHLKQKAKGVRAKPRSAPCRFFRCFRSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YID8</accession>
    <name>A0A164YID8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
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    </protein>
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        <name evidence="9" type="ORF">DCAR_0520402</name>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_017797"/>
    </dbReference>
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    <dbReference type="OMA" id="YAYCKLQ"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM94554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH01024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="336" mass="37103" checksum="FAD46C163A51DC7B" modified="2016-07-06" version="1">MASNNQNLPLYDTKDNKEKPQHASTMSRQGVFAAVSYMLCAVLLIMFNKAALSSYKFPYVNVITLCQMLCSCAFLYVLRRWKIISFTAGESQGKDTNSKILVPLRTVLHTLPLAFSYLIYLIVSMESVRGINVPMYTTLRRTTVAFTMLFEYLLAGQKHSLSVVGSVGIIIFGAIVAGVGDLAFDPYNYAVVFTANICTAIYLASIARVGKTSGLNSFGLMWCNGLICTPFLLFWTYFSGDLEMTLQFPHLFSPGFQAVMVLSCLMAFSLNYTVFINTTRNSALTQTICGNLKDLFTVGIGWLLFGGLPFDLKNVIGQSLGFLGSCVYAYCKLKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZS65</accession>
    <name>A0A164ZS65_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_019568</name>
        <name evidence="5" type="ORF">DCAR_0522386</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZS65"/>
    <dbReference type="Gramene" id="KZM96326">
        <property type="protein sequence ID" value="KZM96326"/>
        <property type="gene ID" value="DCAR_019568"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220892"/>
    <dbReference type="OMA" id="SKEIYMM"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF456">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ELI1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="217"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM96326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60965" checksum="3F6D063C2D404910" modified="2016-07-06" version="1">MSSLATFCTAPPPQSSTVKHHHNHLSPARLATLIDNSKTIKQLFQIHAYLLRNSLETHPILNLKLQRSYSLLGRLDYSSTLFHRTLDRDVFFYTSIILSHNNENLHWQAMGLYVEMLSKKIEPNAFTFSAMLKNSPIVTARGLHGQAIKMGCEMNSYVRTGLIDVYARGGEVELAGQLFDKMPERSLVSLTTMMTGLAKNGDVDGARAVFEGMGERDVVCWNVMIDAYVQHGRANEALVLFREMLSGDVCPDGVTLVAVFSACGQLGALESGRWVHGYMKNRRVRMNVHVGAALVDMYSKCGSWEDARLVFDEIKSKDIVVWNAMISGYAMNGLSREALQLFSEMRQLSVHPTEITFIGLLSVCAHAGLVREGSEIFRSMRKEYGIEPRIEHYGCMINLLGRLGHLNEAFKLVKAMTMSIATRPDQIIWRTLLDACRLHGDSDLGEDIIKFYTDQNPASSGAFILLSNIYAAKRNWNGAKRVRTMMKDTGIQKEPGCSSIEVNNTVHEFLAGDAKHPKRKEIYLMLEELNRCLRDYGNDAQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YGM5</accession>
    <name>A0A165YGM5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417452</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YGM5"/>
    <dbReference type="Gramene" id="KZM99067">
        <property type="protein sequence ID" value="KZM99067"/>
        <property type="gene ID" value="DCAR_013571"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219265"/>
    <dbReference type="OMA" id="YVWEPSP"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000331">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG98111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="378" mass="42132" checksum="05F511BDA7749A0A" modified="2016-07-06" version="1">MAATAAHQFAQCTTCHAWSPDHSMIAFCPNSNEVHIYRLLQEKWEKMHVLQKHDQIVSGIDWSISSNRIVTVSHDRNSYVWNQEGADWVPTLVILRINRAALCVQWSPRGNKFAVGSGAKTVCICYYEQENNWWVSKLIRKKHDSSVTSVAWHPNNILLATTSTDAKCRIFSTFIKGVDKRSSGAGSSSDAKFGEQIVQLDLSFGWAFGVKWSPSGKTLAYTGHNSVIHFVDDIGPSPSAQTVAFRDLPLRDVLFVSERMVIGVGYDCNPMVFAADERGIWSFIRFLTERKTASAGVKYGSQLSGAFGKLYGQSKYSTGNDTVQVSIPDGNVHENCINCIMPLNKAKDSTTMRFSTSGMDGKVVIWDLDNQLDLHEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A5P0</accession>
    <name>A0A166A5P0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_009523</name>
        <name evidence="11" type="ORF">DCAR_0310764</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A5P0"/>
    <dbReference type="STRING" id="79200.A0A166A5P0"/>
    <dbReference type="Gramene" id="KZN00769">
        <property type="protein sequence ID" value="KZN00769"/>
        <property type="gene ID" value="DCAR_009523"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215174"/>
    <dbReference type="OMA" id="IPINPIY"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="UniPathway" id="UPA00372">
        <property type="reaction ID" value="UER00547"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106286">
        <property type="term" value="F:(E)-caffeate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050563">
        <property type="term" value="F:trans-feruloyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05904">
        <property type="entry name" value="4CL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF413">
        <property type="entry name" value="PEROXISOMAL OPC-8:0-COA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="40"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="452"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN00769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG91515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="541" mass="58371" checksum="14539167D3E7AB9F" modified="2016-07-06" version="1">MALDPRSGFCKANSTFYSKRKPIALPCHQAIDVTTFISSQSHHGHVALIDAATGREITFPQLWAAVESVATALSNLGVRKGDVILLLSPNSIYFPIVCLSVMSLGAIITTTNPLNTTAEIAKQIADSKPKLAFTVPELLSKLADSKLPIVLIGDNSITNSKIIANFESMMRTEARQNRVKDRVTQDDTATLLYSSGTTGASKGVVSSHRNLIGMVQTITGRFQLEQGAQAFICTVPMFHIYGLAAFATGLLASGSTVVVLSKFEMHEMLSAIEKYKATYLPLVPPILVALVNNADQITKRYDLSSLQSVLSGGAPLSKEVIEGFVQKFPSVAILQGYGLTESTGIGASTDSLDESRRYGTAGMLSPSMEAKIVNPETGEPLGVNRTGELWLKGPTIMKGYFSNAEATANTLDSEGWLRTGDLCYIDDDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPEIADAAVIPFPDEEVGQFPMAYVVRKTGSDISGSAVMNFVAKQVAPYKKIRRVAFVASVPKNPSGKILRKDLIKLATSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ACS4</accession>
    <name>A0A166ACS4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0311080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ACS4"/>
    <dbReference type="Gramene" id="KZN01058">
        <property type="protein sequence ID" value="KZN01058"/>
        <property type="gene ID" value="DCAR_009812"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214899"/>
    <dbReference type="OMA" id="MMVEYFE"/>
    <dbReference type="OrthoDB" id="18786at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019465">
        <property type="entry name" value="Cog5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048485">
        <property type="entry name" value="COG5_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049176">
        <property type="entry name" value="COG5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228:SF3">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20649">
        <property type="entry name" value="COG5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10392">
        <property type="entry name" value="COG5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG91825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="824" mass="91045" checksum="F2BF63088C45D57B" modified="2016-07-06" version="1">MASPATPRQYPLQRISTFKDRPSTTASTPSSPLSSFASDPIFSVFLSADFNSTSFSSTALSTGSSIAIAEKLQLAIRTLENQLRSQVLSHHTHLLSQLSNLHQADSSLTHLRNSLSTLQSSLLRLRAEITEPNKAIKSKTTQLSNLHSSVSYLQGTIRLIRLSRKLRGLMGVESLDLSKCAQLYYEVVSLFNESDLGGIGVVEEEMKWVLECGEELRVKGMEVLERGLESFNQAEVGAGLQVFYNLGELRGTVEGLVGKYKNVGVKSIANALDMKAISSGGGMYGGGPGGIQRSGTPQIGGGSKAKEALWQRMNGCMDQLHSIVVAVWHLQRVLSKKRDPFTHVLLLDEVMQEGDSMLTDRIWEALVKAFASQMKSVFTASSFVKEIFTTAYPKLFSMIENLLERISRDTDVKGVLPAMSPEGREQMINAVEIFQTSFLALCLSRLSDLVNTVFPMSSRGSIPSKEHISRIILRIQEEIEAVHMDARLTLLVLREISKVLLLVAQRAEYQISTGPEARQITSAATSAQIKNFTLCQHLQEIHTRVTLMVKGLPAIAAEVLSSSLGTIYGVACDSVTSLFQAMLERLESCILQIHDQNFGALGMDSAMDNNSSPYMEELQQCITHFRREFLSRLLPTSASASSVGSETICTRLVRSMASRVLIYFIRHASLVRPLSESGKLKMARDMAELELAVGQNLFPVEQLGAPYRALRALRPIIFLETSQLAESPLLQDLPPSVILHHLYSRGPEELESPLQRNKLTYLQYSLWLDSQGEDQIWKGIKATLDDYAVKVKARGDKEFSPVYPLMIRLGSSLSEKAPASHKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AUL5</accession>
    <name>A0A166AUL5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0312156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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    <sequence length="536" mass="58968" checksum="225891BB7C41556C" modified="2016-07-06" version="1">MEEAPVTAPRKKMTKQLTGKRDDNQLHSACRAGNVSLMRKLFSECNEEELAELLSKQNSSGETPLYVAVEYGYVELVRELIEYYDLATAGIKARNGFDALHIAAKQGDLDMVKVLMEAHPELSMTVDMANTTALHTAATQGHREVVYYLLEMESSLANIARSNGKTALHSAARNGHVDIVKALLSKEPGLATRTDKKGQTALQMAAKGQNMEVVEELIRADPSSINMTDNKGNTALHTATRKGRVQIVRVLLAQSETDKKIVNRSNETALDTAEKTSQAEIAPILQEHGVQSVKSMPKANPARELKQTVSDIKHEVHHQLEHTRQTRKRVQGIAKRLNKMHSEGLNNAINSTTVVAVLIATVAFAAIFTVPGQYVDDPKDVTPGTSLGEANIAPRVSFLIFFIFDSIALFISLAVVVVQTSVVVIESKAKKQMMAVINKLMWLACVLISVAFLALSFVVVGEHERWLAIGVTIIGTTIMATTLGTMLYWVIMHRIEASNLRSIRKSSRESRSQRSLPASINSDTELANEFKKMYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FGI1</accession>
    <name>A0A166FGI1_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008595</name>
        <name evidence="3" type="ORF">DCAR_0209711</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FGI1"/>
    <dbReference type="Gramene" id="KZN07758">
        <property type="protein sequence ID" value="KZN07758"/>
        <property type="gene ID" value="DCAR_008595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN07758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="23614" checksum="5F94A6C935506A58" modified="2016-07-06" version="1">MGDTGKRTQSGDLSQSTVRKRSSDTEESDVQGASPFGSCHQGVHGHSPQSQSTMSQSGVTKKRRGPNDLLFRKSDSGTMANQNKENQTPDTGVQRRGRGLSIENQIRSGICKTTGRNAMHDISNTPLETQTRSTAVHCDSSDNAMNDCAGQRIGESSTMTNKGTNRAMTFSDRYTPSGVKNLMEVFNEEDDQVPFNVPTDYNHGNGPNIGSYHRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FPL0</accession>
    <name>A0A166FPL0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_000698</name>
        <name evidence="3" type="ORF">DCAR_0100490</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG81344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FPL0"/>
    <dbReference type="Gramene" id="KZN08029">
        <property type="protein sequence ID" value="KZN08029"/>
        <property type="gene ID" value="DCAR_000698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9618" checksum="BDF11370D6B13B1D" modified="2016-07-06" version="1">MMGAAEAVALLAAPEMNNWKRTILLTLLVRNTTTIVDRIKSRSVSRSEKRTVKHYKRLIRQRHVQEQPRSEQSKEDDEIWCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GN99</accession>
    <name>A0A166GN99_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_001811</name>
        <name evidence="11" type="ORF">DCAR_0101814</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GN99"/>
    <dbReference type="STRING" id="79200.A0A166GN99"/>
    <dbReference type="Gramene" id="KZN09155">
        <property type="protein sequence ID" value="KZN09155"/>
        <property type="gene ID" value="DCAR_001811"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204443"/>
    <dbReference type="OMA" id="STFQQKE"/>
    <dbReference type="OrthoDB" id="2011723at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048767">
        <property type="term" value="P:root hair elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000001">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN09155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG82648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41686" checksum="1CC34399FA4E5E0B" modified="2016-07-06" version="1">MADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDGLMKILTERGYMFTTTAEREIVRDMKEKLAYVALDYEQELETAKSSSAVEKNYELPDGQVITIGAERFRCPEVLFQPSMIGMESAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKGEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IGJ4</accession>
    <name>A0A166IGJ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L18 ribosomal protein Heart Stopper</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_003765</name>
        <name evidence="5" type="ORF">DCAR_0103996</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IGJ4"/>
    <dbReference type="STRING" id="79200.A0A166IGJ4"/>
    <dbReference type="Gramene" id="KZN11109">
        <property type="protein sequence ID" value="KZN11109"/>
        <property type="gene ID" value="DCAR_003765"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194004"/>
    <dbReference type="OMA" id="FHNGQVH"/>
    <dbReference type="OrthoDB" id="1932324at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000004">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF5">
        <property type="entry name" value="RIBOSOMAL L18P_L5E FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN11109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG84811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20628" checksum="578D73477276E342" modified="2016-07-06" version="1">MLKQVHNKLFARGSDKILPALYMLRKGLHCGQPRLAPRSFFGVEDFLDDNNSRPYTYQKEKKSKNPNKHVSFKQRTVAYMEPFTLDVFISKRFVSASITHRVTSKQVAVAGTNSKDIKAALKSRSDIPACLSVGKILSERAREADVYTASYTPRDRDKFEGKIRAVVQSLIDNGIDIKVYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IPR8</accession>
    <name>A0A166IPR8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_004011</name>
        <name evidence="5" type="ORF">DCAR_0104273</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IPR8"/>
    <dbReference type="STRING" id="79200.A0A166IPR8"/>
    <dbReference type="Gramene" id="KZN11355">
        <property type="protein sequence ID" value="KZN11355"/>
        <property type="gene ID" value="DCAR_004011"/>
    </dbReference>
    <dbReference type="OMA" id="NWRDQNA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018687064" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="3">
        <location>
            <begin position="41"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN11355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG85086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="173" mass="20023" checksum="148E2C703133500E" modified="2016-07-06" version="1">MASPYALCMALFLLIMGLLLPYEVSSSRAGPQLGKTMFERHQEWMLQHGRVYRDAMEQEARFKIFKENVERIEAFNSDPNRRFTLAVNHFADLTDEEFRVTHASGYNRSMTRTASFSGSSVRPEFYGDYGRPRRSYFRYANVQGVPPSVDWRQKGAVTPIKYQGSCGKCFRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IUD6</accession>
    <name>A0A166IUD6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IUD6"/>
    <dbReference type="Gramene" id="KZN11500">
        <property type="protein sequence ID" value="KZN11500"/>
        <property type="gene ID" value="DCAR_004156"/>
    </dbReference>
    <dbReference type="OMA" id="RDCETAG"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF38">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOTIDE TRANSPORTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG85248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="418" mass="46078" checksum="00187B14AAE6FA00" modified="2016-07-06" version="1">MPASSETPVRLEGTLLAILVCWFLGLGSLVSWNSMLTIGDYYYALFPDYHPSRVLTLVYQPFALGTMAILAYNESKIDTRKRNIAGYILFCLSTFALIVLDLATSGKGGIGNYIGICAFVGAFGVADAHVQGGMVGDLSFMRPEFMQSFFAGLAASGALTSGLRLMTKAAFENSGDGLRKGTMLFLAIATFLEFLCIFLYALVFPKLPVVKYYRSKAASEGSTTVSSDLAAAGIQKEEIQQAEDDADSKKRLTNKELFYQNIDYLLDLFLIYVLTLSIFPGFLYENTGEHQLGSWYPLVLIAMYNVLDLIGRYVPLIEFIKLESRKGLMIAILSRFLLIPAFYFTAKYGDQGWMIFLVSFLGLSNGYLTVCVMTVAPKGYKGPEQNALGNLLVLFLLGGIFAGVSLDWLWLINSSTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IUV1</accession>
    <name>A0A166IUV1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IUV1"/>
    <dbReference type="Gramene" id="KZN11516">
        <property type="protein sequence ID" value="KZN11516"/>
        <property type="gene ID" value="DCAR_004172"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213986"/>
    <dbReference type="OMA" id="WYVKMVE"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010019">
        <property type="term" value="P:chloroplast-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF5">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="391" mass="44416" checksum="4FD048DC2B0BE5EF" modified="2016-07-06" version="1">MSSFGRLYKFFSTAAPAVSETTISATKQAIATTPKPNKPTKPTSQAARLKQLAKNFKKSSKNPSFRLRIPYYESTVYRLAAAKQFNLIQDILEHQKQFDSITDERFTVRLISLYGKAGMFDNASKLFDEMPELKCERTVISFNALLAACVNCKKFDKLEALFRDLPGQLGIKPDRVSYNTLIKSYCVMGSVELAVGVIELMESKGLAPDLITFNTIFDALYRNKKMDDAEKLWVLMESKNVIANVRSYNPKLRALVADERISEAVELIEEMRAKGVMPDIYSFNALIQGYCFEKNLEQAKRWYGELAKCDVSPDRMTYEIMLPFVCDIADFDYAFQLCKDVLNSKHRVSMSVIQRVVDGLATDLKSEEAEVLVDLADTNNYFHYKLKLPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IZI3</accession>
    <name>A0A166IZI3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ROOT HAIR DEFECTIVE 3 homolog</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein SEY1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104637</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="9"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IZI3"/>
    <dbReference type="Gramene" id="KZN11658">
        <property type="protein sequence ID" value="KZN11658"/>
        <property type="gene ID" value="DCAR_004314"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217600"/>
    <dbReference type="OMA" id="WAIVLMI"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002271">
        <property type="entry name" value="Protein ROOT HAIR DEFECTIVE 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF20">
        <property type="entry name" value="PROTEIN ROOT HAIR DEFECTIVE 3 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="681"/>
        </location>
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        <location>
            <begin position="703"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="706"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="727"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="760"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
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        <location>
            <begin position="783"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="833" mass="93898" checksum="DAC00B188679CD0E" modified="2016-07-06" version="1">MDKESCATQLIDGNGEFNMEGVNDFVKRIKLGDCGLSYAVVAIMGPQSSGKSTLLNHLFHTNFKEMDAYRGRNQTTKGIWIAKAVGIEPCTVVMDLEGTDGRERGEDDTTFEKQSALFALAVSDIVLVNMWCHDIGREHAANKPLLKTVFQVMMRLFSPRKTTLLFVIRDKTKTPFEYLEPLLREDVQKIWDTVSKPVAHKYTPLSEFFNVEVTALSSYEEKEEQFREQVAHLRQRFFQSISPGGLAGDRRGVIPASGFSFSAQKIWEVIKQNKDLDLPAHKVMVATVRCEEIANEKYMRLANDKSWLELEKAIQAGPISGFGEKLSSILETFFSEYDSEAVFFDESVRDAKRKDLELKALQLVHPTFMTMVGHLRSKSLHNFKLRLEESLHKGEGFAAAVRTCTKSCMLDFDSGCADATIKQADWDVSKTREKLCRDIEAQAAFIRNEKLSELIARYEKKLNTALSEPVQLLLESGAINTWASIRNLIKHETDATVEGLCAAVTGFELDQSAFDKLVQNMRSYARGLVEKKARDEAGKILSRMKDRFATVFNHDEDSLPRVWTGKEDIRTITRDARVASIKLLSTMAVIRLDEREDNIETVLLSSLLDGPVAVASPRYKNMGTSGDPLASSTWDKVPPEETLITPVQCKSLWRQFRMETEYTITQALSAQEAYKHSNNWLPPSWAIMAMILLGFNEFMILLRNPLYLLVLFVIFLLSKAMWAQMDIARDFQNGTLAGLLALSSRFLPTIMNLLRRLAEEGQGNPTPQPPQRPPRPIRHQSFKNDNQQSASVSSTMADTALSSNVTSVDDDPEYSSPQLTHRHVSRGDNIETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WEE2</accession>
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        <name evidence="2" type="ORF">DCAR_0313637</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="DCAR_012562"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210604"/>
    <dbReference type="OMA" id="ANIGWDG"/>
    <dbReference type="OrthoDB" id="4206278at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325:SF39">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC8"/>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="245" mass="26811" checksum="FA731938419EFE23" modified="2016-07-06" version="1">MGTKMKGLLKGLKQISNKFDNKGEEIQIGLPTDVKHVAHIGWDGPSSLESPSWMKEFKGGAAQSEPLDFSAPPAEDSEKKWVSQDSSRKKSIKSRNLPELPKSSRRQFSESPATSPTPSRDPSKSRNPRRHPRKESDESSRGNRRSKELSPDAPDQGDVPKKTRRKKPKDSSAHRSPKPKDQDNEPAVDGSSQESLPSNTVSDPFSEPGSVDIDSKPMAAGAELLPASKLQSFVPEVQKPNVEVA</sequence>
</entry>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH04075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_031854"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="15"/>
            <end position="81"/>
        </location>
    </feature>
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            <end position="290"/>
        </location>
    </feature>
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            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="398" mass="46204" checksum="F764242B9BD0CDBF" modified="2016-09-07" version="1">MDSLSTLQPGPKVDGKIRVRVSRCWRHVSRSGELMGVSFIVVDHHASRMHGWIRTALTDRFEHQFVEGRVIDIQNFVVRPYRELESNICFRSDKHMMLTGITAIVPVEEVVPNFPMHVFFCNPLNLIQDHGDQERYLIDVVGTVRSAQNLKRYIDKNGTEQSFVRFILANNDDTTVSVTLWNNLANSFMAQTEGFEAYPITVLISSCKVLMHRDLQPTTLTNWRIRVRVSRKWCNTFGGSTKTGVNLIFVDASDSRMHAWISSRVAQFFENKFAEGEVVHIYNFRVRSYRPYVTERCFWNDKYIYITTNTQVVEVQSPCITIPEHVFDSVPLFSLSGFVNRTLHFTDLIGIIHMERPLRHYVDIYNIPQALIKFKVADRRYWCCTIYGNTVTIICDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YEU2</accession>
    <name>A0A175YEU2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_029735</name>
        <name evidence="4" type="ORF">DCAR_0934309</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YEU2"/>
    <dbReference type="STRING" id="79200.A0A175YEU2"/>
    <dbReference type="Gramene" id="KZM82166">
        <property type="protein sequence ID" value="KZM82166"/>
        <property type="gene ID" value="DCAR_029735"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202871"/>
    <dbReference type="OMA" id="RFNCSKQ"/>
    <dbReference type="OrthoDB" id="515654at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35294:SF4">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35294">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED/TRANSLATION ELONGATION FACTOR EF1B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="149"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM82166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="632" mass="68848" checksum="F173F805343E2303" modified="2016-09-07" version="1">MSPATKSKSKSKEKPLTKATKELQKASQKYPESNNGSTPASAYNPLSGTFHSLDSASGTSSPPLQSNGRFRNIEETDDHSGNLHRTAIEYDSLSNNDSCSGESEDHKEKATTTAIRPDVVTVSDNEKREKIRQKNERKHQRQRERRAQELHERCSGYLMSRKLESLSQQIVAMGFTSERATMALMLNEGRLQESVNWLFEGTEQESQTKDIGSGANLKIDIAEELARISEMEVRHKCSKQEVERVVVSCEGDLDKAEETLNSQKPEPITHPKPEESASLKHAMMHQEKPTSAVTTLLNRNEGEMAYSKGAVALPTHPATGSRNQLSSKIIPSKPQTEKRVSGNYNSSLSYSSTGPIQVASSSAKVMAQLGVGLEGRPFHQGAAIKEPIVMMQRPQSINIKPSSVINSSASPPGTNQWYGNNVMGVDNMILNGKMVHSQSSGNFGTGSQHFYPQSQSRNLGPDNPSSRQYNTQAQYMQQRAYMSNSVDPVAARPVAPSWSPVGTSSSPSLTVPSSLGLFSGFGSGATSGLPSHVDWNARDMMSHCDYNSIDWTLKSNPMSPPKLNDLMLEDRSGGLMRGLSSMHSTNGVRVAGLQDGGPTEATTSGVPREWTSPFAGKDIFSLPRQFVTSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YEU7</accession>
    <name>A0A175YEU7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">soluble epoxide hydrolase</fullName>
            <ecNumber evidence="2">3.3.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_029383</name>
        <name evidence="10" type="ORF">DCAR_0933853</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Epoxide hydrolase involved in the biosynthesis of cucurbitacin and mogroside tetracyclic triterpene natural products (e.g. siamenoside I and mogrosides IV, V and VI). Cucurbitacins have cytotoxic properties and exhibit deterrent taste as a defense barrier against herbivores. Mogrosides are nonsugar highly oxygenated compounds used as high-intensity zero-calorie sweeteners; they also possess pharmacological properties such as regulating immunity, lowering blood sugar and lipid levels, protecting the liver, and acting as antioxidants and antitumor agents. Catalyzes the hydrolysis of aromatic epoxide-containing substrates, such as the conversion of 24,25-epoxycucurbitadienol to 24,25-dihydroxycucurbitadienol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(24S)-24,25-epoxycucurbitadienol + H2O = (24R)-24,25-dihydroxycucurbitadienol</text>
            <dbReference type="Rhea" id="RHEA:81855"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:229949"/>
            <dbReference type="ChEBI" id="CHEBI:229950"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:81856"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19038"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YEU7"/>
    <dbReference type="STRING" id="79200.A0A175YEU7"/>
    <dbReference type="Gramene" id="KZM81770">
        <property type="protein sequence ID" value="KZM81770"/>
        <property type="gene ID" value="DCAR_029383"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201900"/>
    <dbReference type="OMA" id="NWYRVRT"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000161">
        <property type="entry name" value="Epoxide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM81770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH14334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="36216" checksum="025E3328B50057B4" modified="2016-09-07" version="1">METIEHKLVSVNGITMHVAEKGTGPVVLFLHGFPELWYTWHRQILAFAASGYRAVAPDLRGYGDTDAPEAVTKYSCHHVVGDIVALIESLGVDKVFLVAHDWGAMIGWYLCLFRPDMVTAFVPLTVPFRPRNPRWKPVEAMKAFFGDDYYMCRFQEPEIEAEIKKYGTANYIKKVIASRKPGPPCLQKENPFGNPDTDTPVTLPSWFSEEDLKYYVNKFEHKGFSGPLNYYRTLDLSWELTAPWTGSKVKVPVKFMVGDEDVIYTTPGTKEYVHSPSFKKDVPLLEDIVILEGVAHFLHQEKPEEVNAYIHNFFKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIN6</accession>
    <name>A0A175YIN6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_031143</name>
        <name evidence="4" type="ORF">DCAR_0936130</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM83574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YIN6"/>
    <dbReference type="Gramene" id="KZM83574">
        <property type="protein sequence ID" value="KZM83574"/>
        <property type="gene ID" value="DCAR_031143"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044670" evidence="2">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM83574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH16574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="100" mass="10914" checksum="F70664DEED9E4F7C" modified="2016-09-07" version="1">MAFVAKPNIFVLVFILLSIYGALSTSATSLHQRHEKQNSRWFSDCLSVHRKLGLLDCGGGSANMNRANYWSESLKTSQSPPPAPVKRVATRSGIASPPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMS6</accession>
    <name>A0A175YMS6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_027921</name>
        <name evidence="2" type="ORF">DCAR_0831527</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YMS6"/>
    <dbReference type="STRING" id="79200.A0A175YMS6"/>
    <dbReference type="Gramene" id="KZM84657">
        <property type="protein sequence ID" value="KZM84657"/>
        <property type="gene ID" value="DCAR_027921"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM84657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="61" mass="6963" checksum="51E1EB4E5E79259B" modified="2016-09-07" version="1">MCFDTVWPITCSTKFLTQVNSLDEPRVVEPMRLPTIEEVRGQDIWNNCDVRGVTTGVMGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YN09</accession>
    <name>A0A175YN09_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0831077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YN09"/>
    <dbReference type="Gramene" id="KZM84222">
        <property type="protein sequence ID" value="KZM84222"/>
        <property type="gene ID" value="DCAR_028231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="522" mass="58138" checksum="364CBEC1B17B517F" modified="2016-09-07" version="1">MSSSSPPKNPSPVSSGSASPDFGLTEAKSENVGNQEVSSEPQAGPSNVYSPQPLSSYDPHQPHLAPQPISSSQPNLDGLNMSLEMDPDTKQLIQSQGITIAEYTQQRRAFAKYEHPAIPNIYSGLDEETANVIHSEEMSPSDLRRQQQALDYFNRMKESLHQDPAGISRIESQIQEPYALLSNNSVLGVDTMGSYLDPNEELDPNDPFFEELADLVDPVNERIKACFGPEIGSTLASTSRVSAKGKTLIIKGPKPQSVKDTKSKISEGQPVPEIDNWPWKRKMTDTLPEAIEDKQSKRRKLLESPLEHGENQEEERCLLLFKDDENDDDYETLYRLYWYTLNQRTSIFQEGDFVQDFENGLTKYINEFLDSKITTEQCKKKVMELRKKYLYFMELNGYEDFNEADFSVPREYKMFQMFKIVWGADPGHGGSCSVAGQGVNVGNPLQGAVDNMNVDQGHVAEPIPPALTNVSGAGKAPEPANVKSGAADKELEADMARAVSNSLKEVRDPSADQKDDNMDIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQM7</accession>
    <name>A0A175YQM7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0833108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the patatin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQM7"/>
    <dbReference type="Gramene" id="KZM86026">
        <property type="protein sequence ID" value="KZM86026"/>
        <property type="gene ID" value="DCAR_026552"/>
    </dbReference>
    <dbReference type="OMA" id="SIDWINP"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF99">
        <property type="entry name" value="PATATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="400" mass="43480" checksum="66AE159BC3DD636B" modified="2016-09-07" version="1">METKGILVLSLLLALATTLECAHKSPGIKTTKAKTATVLSIDGGGIRGIIPGTILAFLESKLQELDGPTARIADYFDVISGTSTGGLVTAMLTVPDKDNRPLFAATDINKFYFENGPRIFPQDNVSAKGPKYDGKYLRSIIRESLGNIAMNQTLTEVIIPTFDIKLLQPTMFSTSDGEVSTSKNALLADVCIATFSNPTFLPAHYFETKFEDGKTRSFNLIDGGVVAVNPTQVAITHLFNEIVKGNFEYADIKPMDTTKILVLSLGTGTAKFEEKYNASMASKWSSIDWINPIIDSYSASSADMPDIEVTSLFQSLGAEKNYLRIQENNLVADTTSGDLATTENMKTLENIGNRLLEKSVARVNSDTGAYEPVEKGGTNSDALIRFAKLLSDERKIRKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W391</accession>
    <name>A0AAF0W391_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W786"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="312" mass="35013" checksum="9087ED6E42BA8C7A" modified="2024-05-29" version="1">MVFGQVVIGPPGSGKTTYCNGMSQFLPLIGRKVAVINLDPANDSLPYDCAVNIEDLIKLSDVMNEHSLGPNGGLVYCMDYLAKNIDWLQSKLEPLLKDHYLLFDFPGQVELFFLHDNAKSVIMKLIKKLNLRLAAVHLIDSHLCTDPGKYVSALLLSLSTMLHLELPHINVLSKMDLIESYGKLGFNLDFYTDVEDLSYLQNLLAQDPRSAKYRKLTKALCDVIEDFSLVNFSTLDIQDKESVGNLVKQIDKCNGYIFAGIDASAAEFSKIAVGPVDFDYYRYPFICFFSLFTVAAVQEKYVKDDDNFDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8F5</accession>
    <name>A0AAF0W8F5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein MOR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein GEM1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein MICROTUBULE ORGANIZATION 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0104393</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202291"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000121">
        <property type="entry name" value="Protein MOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000137">
        <property type="entry name" value="Protein MOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000155">
        <property type="entry name" value="Protein MOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000165">
        <property type="entry name" value="Protein MOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="275"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="447"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="590"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="846"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="1016"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="12">
        <location>
            <begin position="1168"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="500"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1077"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1401"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1759"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1844"/>
            <end position="1865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1959"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1092"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1401"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1972"/>
            <end position="1985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG85205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="2024" mass="222716" checksum="1A1CEECC3A428766" modified="2024-05-29" version="1">MSTEDEKLLKEAKKLPWEDRLTHKNWKVRNDANIDLAAVCASISDPKDPRLRDFGPFFRKTVADANAPVQEKALDALIVFLKVADADAGRYAKEVCDAIVAKCLTGRPKTVEKAQAIFMLWVELEAVDAFLDAMEKAIKAKVAKAVVPAIDVMFQALSEFGSKVVPPKRILKMLPELFDHQDQNVRASSKGVTLELCRWIGKDPVKSILFEKMRDTMKKELEAELANVTGSARPSRKIRSEQDKEPEPEAMSEVAGSGPAEEAAADTPQEIDEYELVDPVDILTPLEKTGFWAGVKATKWSERKEAVAELSKLASTKRIAPGDFTEICRTLKKLITDVNIAVAVEAIQAIGNLARGLRTNFSGNSRFMLPVLLEKLKEKKPTLTDALTQTLQAMYKSGCLNLADIVEDIKTAVKNKVPLVRSLTLNWLTFCIESSNKAVLLKLHKEYVPICMECLNDGTPDVRDAAFSALAALAKLVGMRPLENSLEKLDEVRKKKLSEMIGSSGGGQPVAAGSAAVKSSSGSMPGSESSDASYVRRSAASMLSGKRPVQAAAANKKVGSVKSGASKKGDGLGPSKAPKAIEPEDVEPADMGLEEIESRLGSLIQAETIALLKSAVWKERLEAIVSLKELVEAIQNIDASVEILIRLLCAVPGWNEKNVQVQQQVIEVTIHIASTASKFPKKCVVLCIQGVSERVADIKTRAHAMRCLTTFCESVGPGFIFERMYKIMKEHKNPKVLSEGLLWMVTAVDDFGVSLLKLKDLIDFCKDVGLQSSAAATRNATIKLIGALHKFVGPDIKGFLSDVKPALLSTLDAEYEKNPFEGAAAAPKKTVKLADSSACASGGGLDSLPREDISGKITPTLLKGLESSDWKIRMESIEAVNKILEEANKRVQPTGTVELFGALRGRLYDSNKNLIMATLSTIGGIASAMGPAVEKASKGILSDVLKCLGDNKKHMRECTLTTLDLWLAAVHLDKMVPYFAAALMDAKIGAEGRRDIFEWLSRQLAGLKDFTDAIHLLKPVATAMTDKSADVRKAAEVCFGEILKVCGPELVTKNVRDIQGPALAIVLDRLKPYGASRDTYESTRTTSTAPPSKGGLKSGKSNGAVSKHSRAVAPRGVLAKGARPETIMSVEDINMHSQALLNIRDSNKDERERLVVRRFKFEEPRLEQIQDLESDLMKYFRDDLHKRLLSSDFKKQVDGIDMLQKALPSISRDITEVLDILLRWFVLRLCESNTSCILKVLEFLPELFGTLRNEGYIMTESEAAIFLPCLFEKSGHNIEKVREKMRELTKLIIHTYSAAKTFPYVLEALRSRNNRTRIECVDLVAYLLDNHGPEISGQLKSLQIVASLTAERDGELRKAALNSLATGYKILGDDIWKYVGKVTEAQRSMIDDRFKWKAREMEKRKEGKPGEARAALRRSVRENGPEAAEQSGEIFRSMSGPIVSRENYDQPDLHMERHSMPRALASPIGPTDWNEALDIIVYGSPEQSVEGMKVVCHELSQTMNDPDGSAMDDVLKDADRLVSCLANKVAKTFDFSLMGASSRSCKYVLNTLMQTFQNKRLAHAVKESTLDSLITELLLWLLDERVPRMDDGSQLLKALNVLMLKILDNAERTSSFVVLINLLRPLDPSRWPSPPSNESFAMRNQKFSDLVVKCLIKLTKVLQSTIYEVDLDRILQSIHVYLQELGMEEIRKRAGADDKPLRMVKTVLHELVKLRGTAIKGHLSMVPIDMEPQPIILAYIDLNLQTLAAARMLTPSGPVGQSNWGDSTANNSSQATHSADSQLKQELAAIFKKIGEKQTCTIGLYELYRITQLYPKVDIFSQLQNASEAFRTYIRDGLSQMEKNAAAGRTPSSVPLATPPPSSLNLSSPKFAPLSPVHTNTLSEAKSVNSKVEPTSFSLPPSYAEDDKAYNTMMSRGPISDHQELRHQTGEQNERFPPGVTGGTLNAIRERMKSIQAAAGNPESGNRPLMNLNGSVDPSQISQSTDRANFENPAQVGVLPMDEKALSGLQARMERLKSGSIEPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8H0</accession>
    <name>A0AAF0W8H0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0103297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8H0"/>
    <dbReference type="KEGG" id="dcr:108204818"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="13"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="33"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="86"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="155"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="218"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="282"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="399"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG84116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="564" mass="61284" checksum="7F3F199C9ACA726F" modified="2024-05-29" version="1">MDVGKHQDSGTLSEKIIQIAAGESHTLALTGDGSVYSWGRGMFGRLGTGSEEDKLTPTRVNFDLDKVKIVGIAAGSYHSLALADNGSVWSWGYNSYGQLGYEGENSSLPHLVEGFHDLESPDSITDRSKTKTTPSLKISSVKAGGMMSLAIDNLGALWMWGNCPQNILGSEAEALPSSSSTPLRVREFRGHTVVKVACGNEHIVALVGACETPNDGDLICYSWGINRQGQLGLGDKETRLSPEVIGSFNNIESSWAVYDVACGGSHTAVLACNKGSPDRSESVCFTFGLGDNGQLGQGTTQGVLYPKPVKVLPKNVFLISVDCGLFHTSVVSSAGDVWSWGMEKGYGLCPDFSFTSTDAGDALYPRLISCTEPYGPRFPEPVQIVCGAAHTILAAAGGYKLWSWGRGQSGLLGNGKTMNCMDPSTVLWPPLTNHDKEEIPDNSSGDKQSILKEQDDTEDKQRLSAALGEMKLLQTKLSTMERYTSILHGSVFGKPFREQDIPITLQNTGTFDLAREWENMLESSDHAKLARLEVFYRSMLSDIKDKLMKKRIKEIIKECLDERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8R6</accession>
    <name>A0AAF0W8R6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8R6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052657">
        <property type="entry name" value="PDP_family_Arabidopsis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10688">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10688:SF3">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="325"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="998" mass="106016" checksum="5BCF62C6F18EA357" modified="2024-05-29" version="1">MGEGVKRGLDIVASGEVGSGNDVILEGGVGIKDDNGDGAKRGFDIVASGEVGSGNGLILEGGVGGKDDKGDGVQRGSDVVTSGEAGSGNGVILEGGVGINDDNGDGAKRGFDIVASGEVGSRNGVILEGGVGGKDDKGDGVQRGSDVVTSGEVGSGNCVILDGGVGVKDDKGDGVQSGSDVVASGEVGSGNGVILEGDLGVKEDSSDGVQRGSDIVASSEVGNGNAVNLESEIGVKDDGVGRVSDLSGEDEEMKSGVGRVSDLGGDGKEMKSGLGNDIGLQVLKGTVAKQKGEVNGVESDESDYGDYDDDGDDTDIGDQSCEYRVGDFVWGKIKSYPWWPGQIYGESDASEYALKLKQKDRFLVAYFGDGSFSWCGPSQLKPFVENFKVMSEQSNSKNFTYAVEAALDEFSKVVERDMACSCLAEKNGKLEASMASNSGLKVGVLVPAGVGISFIAQYNPKKLIDSLRNYAETASMKNLLELTVIKSWLTVYGRAKGGYPLPLYHYPRGIEGLEDKSGNPVKVSAQGPNGDEWLSIPVEAADSGLDLSNQSLLSKCQEASGDIQYQRRKQKSVAELIEDEMRKEEGFVNESVSNVVSSGKRGRRQKAVASRSPRTGGEKITARESKVFGEKEADEPPPSGTLQSKILSGGESEGDEETAKSNRLRERKRSKYLSPPFTNPSQIMKISSFKRDSQTESVKNSRVGERMARVAGQLIGLQSPSQTEFPIVSSKNNAKSGSSSPETALQDDRKTNISMHLTASPVQLLSQLQSAAADPSCLIEKSEVDSITENIISFRNKVQQKGEPGSMKRKPECVFQTIENGIDGQDFKMPPAKRAASGCETMTNVEDASEETPVTLILTFPPAYPLPSRNELIKIYGKFGPVDNSETGVWYNSFSARVSYLRRSDAEEAYKASVEKNPFGDANVNYRLRERSSLDNGKATQSCDDEATQLGLIREKLKMVTSIVETCGGKISVEFKSDLESEVKTLLERVRSMAVTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9Z3</accession>
    <name>A0AAF0W9Z3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
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                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="12">
        <location>
            <begin position="180"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG85778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="508" mass="55111" checksum="F85BEFFB033E634C" modified="2024-05-29" version="1">MAVVASAPGKVLMTGGYLILERPNAGLVLSTNARFYAIVKPLYDELKPGSWAWAWTDIKLTSPQMSRETTYKMSLKHLLLQCTASSDSRNPFVEYALQYSVAAAYANFDSDKKNVLHKLLLKGLDITILGSNDFYSYRKQIEALGLPLSPESLATLTPFTSITLNAEESNGETSKPEVAKTGLGSSAAMTTAVVAALLNYLGVVNLSSSSEDQDMMETTDLDVVHAIAQTAHCIAQGKVGSGFDISSAVYGSQRYVRFSPKVLSPAQDAYEGKPLDEVISEVLKGKWDHERTKFSLPPLMTLLLGEPGTGGSSTPSMVGAVKKWQKSDPQRSRDTWTKLSDANSALEEQLNLLRKLAAEHGDAYQCVINNCSIRKSEEWIEQASEPRQVEIVKALLESRSSMLEIRNHMRLMGEAAGIPIEPVLQTQLLDATMNTEGVLLAGVPGAGGFDAVFAVTLGDASRTNLTKAWSSHNVLAMLVREDPRGVSLESNDPRAREITSALSAIHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ34</accession>
    <name>A0AAF0WJ34_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208256</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJ34"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039306">
        <property type="entry name" value="MYOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448:SF3">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="GTD-binding" evidence="8">
        <location>
            <begin position="570"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="685"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="604"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="863"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG89020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="937" mass="106331" checksum="48B3CE4A41595879" modified="2024-05-29" version="1">MGTNTFASMLNRNTNKFTLILVYALLEWTLIALLLLNSLLSYLIVKFAIYFGLKPPCFWCSRFDHFLQPEKGNNFNRDLLCEAHANEVSVLGYCRNHQKLVACQNMCEDCSSSRPVFHGVSKNLNVFSWVEEIEMIHDDAEKVFDDGVLSFKCSCCDVSLEKQLNSPHILDLECTKKENLMPDEEGNDSVTKRDDFSDGSGSDILVDHFEDDQGVKGKNEEHMIFAFDGGYSLIEGKDDDSDSSVSVSKFGLEELKGGDDDEVEMLLQEESDFFEEQDLSKVMEDLISDEIMSQACRKENVSMKIHPEHLEFFIDFSGHELVPVELIDLNTEENELIKAVENQKYSEFHEEDGSKCEVVARVEASVAEETKFAVFESMEMKETENSLVFYAKENYSENQNVDRSAVMEETGNSMLFHAKLTSPKYGKESHIIVNETEVEVSIGTEIPDLESADETQAQDTICFTEEPSKRSANFHGTEHEHGMEIKLQQEETLRLGNLAIEISENMTANQLSVSTESNGTDEEKICDTPTSLDSFHHLHKKLLLHDGKESGTEESLDGSATDFESGDGVMTVECLTSALRAEKKALHVVYAELEEERSASAVAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSEYDQEALQLLNELMLKREREKQQLERELDLYQKKVMDYEAKEKMRMLKRSKDGSARSGFSSTSFSNGEDSDGISMDLNHEVKEEDGFYSHQVSENHNTPIDAVQHLGDSFGEFEEERMLILEQLKVLEDKLFTLSEDEHRYEDVRPIKDFVHISGNHLDENSDSSGEELNGLANGCSKPTNGIHHQEGRFMGANAKRLLPLFDAIAAENANGELSGDENVFESVVTRFELEERRVAIEEEVDQLYARMHALETDKEFLKHCLSSLKKGDKGMDLLQEILQHLRDLRNMELRVKDLSDGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJM3</accession>
    <name>A0AAF0WJM3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0310335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF15">
        <property type="entry name" value="AGL218WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="136" mass="15304" checksum="25D9B2F766804267" modified="2024-05-29" version="1">MRSNKDEDDDLADRFSELETLASADETQARSASCEGNDELISESELSDGGSDDEVAGCKKELQLSDIKAERSKKKFEQKRSSGKLLNAVITAPALSIKSVLHKWVEDGNEVKKSDTRKIISYFQKRRMYRRALQVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJX0</accession>
    <name>A0AAF0WJX0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="3">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">V-ATPase 69 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Vacuolar proton pump subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0208663</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJX0"/>
    <dbReference type="SMR" id="A0AAF0WJX0"/>
    <dbReference type="KEGG" id="dcr:108209232"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000325">
        <property type="term" value="C:plant-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="15">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="16">
        <location>
            <begin position="127"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="14">
        <location>
            <begin position="232"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="17">
        <location>
            <begin position="466"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG89425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="623" mass="68835" checksum="2CE91AE8D0EFF7E5" modified="2024-05-29" version="1">MPSVYGDRLTTFEDSEKESEYGYVRKVSGPVVVADGMGGAAMYELVRVGHDNLIGEIIRLEGDSATIQVYEETAGLMVNDPVLRTHKPLSVELGPGILGNIFDGIQRPLKTIAKRSGDVYIPRGVSVPALDKDTLWEFQPKKIGEGDLLTGGDLYATVFENSLMQHHVALPPDAMGKITYVAPAGQYSLKDTVLELEFQGVKKQFTMLQTWPVRTPRPVASKLAADTPLLTGQRVLDALFPSVLGGTCAIPGAFGCGKTVISQALSKYSNSDTVVYVGCGERGNEMAEVLMDFPQLTMTLPDGREESVMKRTTLVANTSNMPVAAREASIYTGITIAEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLAARLASFYERAGKVKCLGGPERNGSVTIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPSVNWLISYSKYSTALESFYEKFDSDFIDIRTKAREVLQREDDLNEIVQLVGKDALAETDKITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLANQAVERGAGMDGQKISYTLIKHRLGDLFYRLVSQKFEDPAEGEDVLVGKFKKLHDDLTSGFRNLEDETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLD6</accession>
    <name>A0AAF0WLD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0209787</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLD6"/>
    <dbReference type="KEGG" id="dcr:108207905"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="221"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="465" mass="53596" checksum="CE93ECD28BCFD0D5" modified="2024-05-29" version="1">MIYFTLFVLIISISFSLFFRWTTFINVVKQMMHSIEDHVYVYQLFKVPELNENLQENQFYRKVSTYVNSLSSSIEDSDFTNLFSSEGKDIILYLDDNQIVHDTFLGARISWKYQVLVEDDYQKKKTFVLKMKKRDKRRILQSYLQHIDTVSDEIEERCRRELKLFINTESHQHRRWRSTPLAHPATLNTIVMDSDLKIRIMSDLESFLESKEYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMAKSLNYDVYDVDLSNVSDDSDLKMLLVQTRRKSLIVVEDLDRFLNDSSSSSVSLSGLLNFMDGIVSSCSGDEKIMVFTMNYKELIDPPVLRPGRVDVHIYFPLCDFNMFLTLADTYLGVKDHKMFPHVEETFEITGATLSPAEIAELMVMNRSSPSRAFKSVMTALQINKVAASSKLQGRWIGDSSDQSESSSGILNWKDVSSPAIKEIRKFYGVLSAKIGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM59</accession>
    <name>A0AAF0WM59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA/RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0209689</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM59"/>
    <dbReference type="KEGG" id="dcr:108209099"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000225">
        <property type="entry name" value="Protein SMG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF35">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="3">
        <location>
            <begin position="206"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="921"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG90445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="996" mass="110501" checksum="3B7E76BB0A5D4BB7" modified="2024-05-29" version="1">MTFVDTTSKMSAPSWERAQRLYDKNTELENKRRRAIQAKVPSDPNTWQLMRENYEAIILEDHSFSEKHNVEYALWQLHYRRIEEFRAHFNAAAAASAGSVPPRSAKGPRPDRITKIRLQFKTFLSEATGFYHDLILKIKAKHGLSLGHFSDDFETRIVFEKDEKKSIEMKKGLIACHRCLIYLGDLARYKGLYGEGDSKNRDYAAASSYYLQAASLLPSSGNPHHQLAIVATYSGDELVAVYRYFRSLAVESPFSTARENLIVAFEKNRKSYTQLHKDIEGSASKESPNQTNNRGKGTKETRLQAKEVDLDGFSERAPSIHDIYKAFCTRFVRLNGILFTRTSLDTFGEVLSLVSNLLRELLSSGAEELKFGLDAVDNGLFIVRLVTILIFTLHNVKREAEGQSYADILQRTGLLQNAAVATFELMGHIFKRCSELSDPCASQLLPGLLIFLEWLASFPDIAAGTDIDDKQVSVRSKFWDHCVCLLNKLPLSGSMLTSDGDNDNCFFNMSKYEEGETDNRLALWEDFELRGFLPLHSAHLILDFSRKQSIGHDSKKEKTSRLKRILAAGKALTNLVKVDQKPLSFNVQLKRFVIGVAPQSSEDTSSALYSAGSKSGASIKESLKAPMTDLAISQSEFELYDGDEDDEVILFRPTLSEKRHDEISELVPPGEVVNGMNAFSDETQLYSAPLSAFEVGLHQNSAFSSGPQAPMPVTAFSSGRQSPLPVPNFVSQQMQPNQTSSSSRNQHDFLAKDLEGWSLVESGRVIDLRMQNDMKVSNVASLSLPVQQIISPGYTGIYSQAVGPGTITQNQINPISSSRAPDVPNNFEFVASSGYNGGIIAPKVSSALQAMSMKGPANRPVRHLGPPPGFNSVRPKQGFEPSVAMSGENPSVDNYSWLDGYTHPSSIGHSSLKQSTGYPSHPGFLYSEEGNAPLEGARFPFPGKQVSSAQFGAEDRNGQIPESLNLPNEQMQQQQQSVPPPQQYQGKSFLMNHRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WNB8</accession>
    <name>A0AAF0WNB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0312063</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WNB8"/>
    <dbReference type="KEGG" id="dcr:108214965"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000003">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF5">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="483"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="690"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG92787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="712" mass="81966" checksum="1AAD57BA3C65D64D" modified="2024-05-29" version="1">MSYRQDKFVQFQDWKSEKSSDGLVSTNEGIQSSKFGLSMNAVSRKFRLGFERSSERIVNLQRSFKSHSFSSLLAKGVGSSKTILDPQGPLLQKWNKIFVISCIIALSLDPLFFYIPVINDVKKCLMLDKKLETTACVLRSFTDIFYIVHIIFQFRTGFIAPSSRVFGRGVLVEDASAIAKRYVLSYFLIDILAVLPLPQVAVVIIIPHMRGSDPLHTKNLLKYIVLFQYIPRVLRVFPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVLGAFWYLSSIERETTCWREACQNSKDCIRSSFHCDDKKNFDLLLNASCPIKTPNTTIFDFGIYHDALNSGIVESKDFPQKLFYCFWWGLQNLSSLGQNLKTSTYVWEICFAVFISISGLVLFSFLIGNMQTYLQSTTKRLEEMRVKRRDAEQWMSHRLLPANLRERIRRYEQYKWQETRGVDEENLIRNLPKDLRRDIKRHLCLALLTRVPMFEKMDEQLLDALCDCLKPVLHTEDSYIVREGDPVDEMLFVMRGKLLTVTTNGGRTGFFNSDCLKAGDFCGEELLTWALDPHSSSNLPISTRTVRVLSEVEAFALLADDLKFVASQYRRLHSKQLRHTFRFYSQQWKTWAACFIQAAWRRHCRKKMEESLREEEDRLQTALAKGTGSSPSLGATIYASRFAANALRALRRNSTRKARIPDRVPPMLLQKPAEPDFTAEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQR4</accession>
    <name>A0AAF0WQR4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0313633</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WQR4"/>
    <dbReference type="KEGG" id="dcr:108214661"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052592">
        <property type="entry name" value="LRR-RLK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054:SF47">
        <property type="entry name" value="OS06G0179800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054">
        <property type="entry name" value="RECEPTOR KINASE-LIKE PROTEIN XA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042145355" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="11">
        <location>
            <begin position="27"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="12">
        <location>
            <begin position="30"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="90"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG94340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="94162" checksum="554C691A8AD8EE82" modified="2024-05-29" version="1">MAIFNKPLVLVLFLVFISILPLYITSSPTTEGEALVKWKDTLFNSSSLHSWSLNNLSNLCNWTGITCNPAGMVSELDISGQRLSGTLAHVNFSSFPSLTYLNMSDNYLSGSIPVDIGNATQLQYLDLNNNNLHGNIPSSIGQLTELQQLDLSFNYLNSSIPPELGLCTKLSVLSLYNNFLTGPLPYSFSNLTRISRLYLGGNNLSGELSPHLLNKWTELTSLDISYNTLSGNIPSEIGMLTNLTFLDLSKNHLKGTIPREIGKLNKLQGLFLLGNHLEGSIPATIGDLAKLEILYLEFNNLSGTIPPEIGNLTLLYSLHLYNNQLNGTIPPEIGRLINLTYLDLSNNQLNGIIPPEIGNLTLLLSLLLDNNQLTGELHDSVYHLTILKLGLSSNRFSGSIPPDFGRPNLFYMALARNNFSGEIPPTLCKATSLWILDLSSNELNGSIPSCLGNLSSNLQIVKLSKNQFKGTLPTFCTTNIVILDLSSNMLGGSLPHCLFTNTSINLNILDLHDNQLQGTIPQITANSCKLTTFNINNNHFQGSIPSALANCKQLQILDFGNNKMKDSFPSWMVALPELQILVLRSNRFQGNLKFKKMEHPFSKLRIMDLSHNQFNGNLPINFFDNFKLMGNSDQNIGLNTTDRRQRSYYEASVSLIIKGMEFEVKRILNIYTSIDLSSNKFDGRVPQIIGELKSLRLLNLSHNSFTGHIPSLKNMSTLESLDLSSNQLAGNISWQLTGLTFLSTLNLSENHLSGAIPHGGQFDTFSNDSFLGNLDLCGGPLTKQCKDDELPAEEVDTVGVGWFDWQMILIGYGSGLVCGLSTGYLVFTTFKPLRFVIFIERVQQKLVDRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQX1</accession>
    <name>A0AAF0WQX1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WQX1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF101">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="1">
        <location>
            <begin position="126"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="846" mass="99019" checksum="628B375BF044ED37" modified="2024-05-29" version="1">MLDQHNNLVKSFRRARDMFTSQPNTTFHLRLPAARTRDGREHNIPTESEVGGLIVGDFNEKEFERDVLVRHHTKGIRHIDELHPSYMSLAYPLIHPFGEDGYRLGIPLADKSSQTFKRQSLTMCQYYCFRIQQRNNEGHTLLQGGRLLQQYIVDAYMAVEQERFRWIRTHQSELRTELYSGLMDAIHRGDSDCSTVGKSVVLPSSHTGGPRYRAQNYQDAMAICRSVGYPDLFITFTCNPKWPEINYMLQLIGQKDDTNRVDIMCRVFEIKLQELMRYIKKEEPFGKVMACLYTIEFQKRGLPHAHILLFLHSSMKNPSADYIDKIICAEIPDINVDPDCHNAVKRSMLHGPCGQSNKLSPCMHNGKCTKFFPKEFNEKTTIGEDGFPVYRRRNTGILVEKKGVLLDNRYVVPYNRNLLVKFDAHINVELCNSARSIKYLFKYINKGPDRATAVIQTSDERDEIKAYLDCRYISSCEACWRIFQFNINYRYPSVERLPYHLPGEHSVIFEENRCVENVLAMPGIERTKFTEWLETNKRYEDARELTYSEFPKCWVWNSKEKIWTRRKKGTSIGRIYFAHPSSGERFYMRMLLNLVKGCTSFECIRTVNGVTYPNFKAACYALGLLDDDKEWVDCLSEASVWATGYELRSLFVTILIFCQVSDAPELWRQHSEILSEDMLHLQRRRFHVPNLQLTQQQIESYALAEIEGLMQKLGKSLRDIHGMPQPDSSLTRDLGNRLINEELDYDREHLKLLHEESFRSLNQYQKIAYDSIIIFHHWTWWNWKNIFMEYNRIKIQVRVAYSVGSCNLRYSVVIIAKWTHSTLSIPHPVGRYSRIHLRNQTWNAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WXD2</accession>
    <name>A0AAF0WXD2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">9-cis-epoxycarotenoid dioxygenase</fullName>
            <ecNumber evidence="13">1.13.11.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0417193</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin</text>
            <dbReference type="Rhea" id="RHEA:19677"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32304"/>
            <dbReference type="ChEBI" id="CHEBI:34596"/>
            <dbReference type="ChEBI" id="CHEBI:35306"/>
            <dbReference type="EC" id="1.13.11.51"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin</text>
            <dbReference type="Rhea" id="RHEA:16541"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32304"/>
            <dbReference type="ChEBI" id="CHEBI:34597"/>
            <dbReference type="ChEBI" id="CHEBI:35305"/>
            <dbReference type="EC" id="1.13.11.51"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 9-cis-epoxycarotenoid + O2 = a 12'-apo-carotenal + 2-cis,4-trans-xanthoxin</text>
            <dbReference type="Rhea" id="RHEA:23328"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32304"/>
            <dbReference type="ChEBI" id="CHEBI:51972"/>
            <dbReference type="ChEBI" id="CHEBI:51973"/>
            <dbReference type="EC" id="1.13.11.51"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="15">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.51" evidence="13"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WXD2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045549">
        <property type="term" value="F:9-cis-epoxycarotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009688">
        <property type="term" value="P:abscisic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF26">
        <property type="entry name" value="9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0937">Abscisic acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG97852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="588" mass="65523" checksum="2462ED087D1DF2F1" modified="2024-05-29" version="1">MAASATSASTWAKPKIYSSQRDSPRKTNVNCAIQSSPSILHFPKQSKQKYQTPSIPTTIVQPKHQDSSSSATPKWNFLQKAASMALDAVENIIISQEQSHPLPKTADPQVQISGNFAPVQEQPVQHHLPVTGKIPECISGVYVRNGANPHFEPTGGHHFFDGDGMVHAVKFKDGEASYACRYTETERLIQERNLGRTVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVQITPKGDLRTVERYSFDGQLDSTMIAHPKIDPETNELIALTYNVIQKPYLKYFRVSPDGVKSSDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIQGGSPVVYDKEKVSRFGVLDKYAKDGSGIKWVEVPDCFCFHLWNAWEDKESDEIVVIGSCMTPADSIFNECDEGLESVLSEIRLNLKTGKSTRRAIISPEDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFNGEIKKFFYGDNKYGGEPLFLPRNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFVSEKDLEKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYD6</accession>
    <name>A0AAF0WYD6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0417673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYD6"/>
    <dbReference type="KEGG" id="dcr:108218670"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF21">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG98332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="52261" checksum="169A5CA433C48AF7" modified="2024-05-29" version="1">MIRNKHAVSSENHHHTIKIWEVNEARLASMQQKIVETKPTLLSKSAGSSTCSIFRVPQSFIDINGRCYQPHIVSIGPYHHGEPHLKMIEEHKWRFLGTLLHRTERKGLSLEDYLKAIQPLESEARECYSEVVKFGTDEFIEMMVLDGCFIIELFRKLGDLYPFEPDDPLISMSWVYSFFLRDLLRLENQIPFFILQRLFDLSTLPEEESGKSLAELALNFFNSALQRPDEVLDQHKNLVGKHLLDFVRASLIPPGDPDHMHYNNPSHVIHCVSKLRRAGIKLKPGKSTSFLVIKFSHGVLEMPSISMDDFMSSFLINCVAYEQCHKSCSKHMTTYTTMLDCLINTSKDVEYLWDHDIVENFYGTDSELARLINNMGKDVTLDIDRCYLAKVFNDVNQYYRNNWHVTWASFQYTYFATPWSFISAFAALVLLILTIIQTFYTVLSFVDGMSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYF0</accession>
    <name>A0AAF0WYF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GPI-anchored protein LLG1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0417240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG97508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="381" mass="41164" checksum="B9AB526389E74695" modified="2024-05-29" version="1">MSFKVLSQMLLNIRPYLAMISLQFGYAGMYIVTMVSFKQGMSHWVLVVYRHVVATLAIAPFAFVLERKIRPKLTLSVFLKVMLLGLLEPVIDQNLYYVGMKSTSATFAAATVNVLPALTFIMAIIFRMERVNLRQKPGLAKVIGTSITLSGAMLMTLYKGPVVDILGGYSHRSQHENSGDASSGQHWVAGTIMLLSCTIGWSAFFIVQSMTLKEYPAELSLTSLICVMGMVEGGVVALVMERDMSAWAIGFDSRLLAAAYSGVVCSGIAYYVQGFVNKVRGPVFVTAFSPLAMIITAILGAIILSEQVHLGSLLGAVVVVIGLYSVAWGKSKDENMTSGEGKAQELPVVREDKYKSAHDSFDGATALDTSIALKSALSGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1L0</accession>
    <name>A0AAF0X1L0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0417331</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X1L0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="16">
        <location>
            <begin position="61"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="17">
        <location>
            <begin position="242"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG97990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="442" mass="49137" checksum="13858169D5712AB9" modified="2024-05-29" version="1">MREIISLHIGQAGIQVGNSCWELYCLEHGIQPDGKMPRFLTFHYADNDSSINFSDTTNDSFGTFFSESSSGQHVPRAVFVDLEPNVVDEVRSGKYRQLFHPEQLISGKEDAANNFARGHYTVGREIVDSCLDRVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFNIYPSPQVSTAVVEPYNSVLSTHSLLEHADVVVLLDNEAIYDICRRSLDIERPTYTNLNPINVDITEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQSSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNSAVAAVKTKRTVQFVDWCPTGFKCGINYEAPTMVPGGDLASVKRAVCMISNNTAVAEVFGRINHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGQEGADEEEAEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X297</accession>
    <name>A0AAF0X297_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">caffeoyl-CoA O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.104</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417736</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(E)-caffeoyl-CoA + S-adenosyl-L-methionine = (E)-feruloyl-CoA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16925"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:87136"/>
            <dbReference type="ChEBI" id="CHEBI:87305"/>
            <dbReference type="EC" id="2.1.1.104"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aromatic compound metabolism; phenylpropanoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.104" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X297"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF74">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042206253" description="caffeoyl-CoA O-methyltransferase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG98395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="139" mass="15368" checksum="81855AFD3D2C48BD" modified="2024-05-29" version="1">MPKTPCMHIGVYTGFSLLATILALPVPDDGKNYEIGLPVIEKAGYHGTFDFVFMDADKSNCMNYHMRLIDLVKVCRLIGYDNTLWNGPVAVPTDTPMANYIRNNRDFVIELNSALAVDPMIEICMLSVGDGVTLCPHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X4V5</accession>
    <name>A0AAF0X4V5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase EXL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X4V5"/>
    <dbReference type="KEGG" id="dcr:108220740"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF95">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41181" checksum="B66DA06FA4070B77" modified="2024-05-29" version="1">MQKLLSEKPCTCLAFLYLNGNAPLVVYCCSILWCSLIMGSEATIKLPENVTVPGLIVFGDSIADQGNNNNLSSVMKCNFPPYGIDFTGGLATGRFTNGRTPSDMIAAELGIKELVPAYLDPSLQMKDLSTGVSFASGGTGYDPQTSKLVSVVPLSDQLEMFKEYIGKLKASIGEENTTHVLNNNIFLLVAGSNDLANTYFTLKLRSFQYDILSYADLLVASASDFVQKIYKLGARRIGIFGVPPVGCLPFQRTLAGGNSRICVDTYNQAAQVYNSKLSSQLNFLGNSLPQAKLVYVDIYNPLIDIIQNPQDHGFDVVDKGCCGTGDLEVVVLCNKWSRVCQDKSKHLFWDSYHPTEQGYKVLVDLILKDYINQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X545</accession>
    <name>A0AAF0X545_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0521348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X545"/>
    <dbReference type="KEGG" id="dcr:108220360"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025830">
        <property type="entry name" value="DNA_bnd_dom_ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF82">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13724">
        <property type="entry name" value="DNA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="297"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH01961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="367" mass="41693" checksum="4CCF91988AFB844B" modified="2024-05-29" version="1">MGKYKFKLSDMIPNAWFYKLKYSTRTTKSPNSMQKLKKNNHAKTKSPAYYSSLSSSSGGAAAASQRKSYHITRDLPPTPDKNSASQHSDSPRRSSRKRGSNRKKRRSIRSTSPRLLLSAPVSTDCSCRASHEDCQNDPLVESSYSEDDYSVFPELWSEKEDKPFHKMVSSCHCRAKSDIPIGMTMSKDSKCENFDPILQFELKPIITKINKVKKNDEEVSKIRMISATNDESISHSFYKEQKSVNSVKKLSFNTNSTGVKLKTDSPRTASKRFLQGHHRKSASLGGARRSLAESFAVVKTTQDPHKDFKDSMMEMITENNIRTTKDLEDLLACYLSLNSDQYHGLIIKAFKQVWFEIADMKHITRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X557</accession>
    <name>A0AAF0X557_DAUCS</name>
    <protein>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY, DYW DOMAIN-CONTAINING PROTEIN"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="47855" checksum="EBF2B633F7DC1FC1" modified="2024-05-29" version="1">MSSYSSNYLRLLSLTKLLTSHVNNARHDQALSLFHHIHSTLSLDPFVFPLALKSCAAINRPLLATSIHSHLIKTSHITNNPYIACALIDMYGKCVSIASARQVFDEMPERNVVMWNAMISIYVHCSDQVGKGLELFEVMDVLPNQSTFNSIIAGLSEVEDGSYKAIEFYRRMRALGLKANLITVLALMNACVGMGVLNMVKEVHGYSIRNDIDWHAHFRSGLVEAYGRCGCLDNACVVFRSLQERDVVAWSSFISAYAFHGEARTALKIFEEMEMAKVKPDAIAFLGVLKACSHAGLPDEARMYFARMHDEYGVEPRSDHYACLVDVLGRSGRLHEAYEVIRGMPVKVTAKAWGALLAGCRTYGEVELAEIAGRALFELEPDNAANYVILSRIYGNVGRRDEAQKIRREMEKRKLKVAPGSSWVFNHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X7D4_DAUCS</name>
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        </recommendedName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOH02530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH02530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="334" mass="37307" checksum="C53621777F438DB6" modified="2024-05-29" version="1">MLSSQVKLNHICWLIQLNSAEPDTSRWLSIDCGADGLPFDDDLIIWDTDDGYIQSGLNELVRTKTSRNELNTLRAFPDKAVEHCYNVSAVTQTIRYIIRLGFYYGNYDGLSKPPRFDLFINHVKWTTVDTSINNGLPFYEEIIYQNEKSGSFKICLVQIKDGGMYNRIWSKDATPYTRVTGFPDFNTYENNPPITILEDAIVANGSDPITLIVNLPQSTQQSAYIVLYLTNLGNPFDSNQTTTLKFEINSQDQGTVNSTGNGKTTVVAKYPVTVSGPTINITLSLADEFSLPPMISAMEVFTKWDTGPSKSTAAAEFFSFAYSLILLCMLVLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X827_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0622905</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH03506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108226522"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF2">
        <property type="entry name" value="OS06G0560700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH03506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="386" mass="42142" checksum="034AC186A8B89AE4" modified="2024-05-29" version="1">MAAINLLPPILFISLCFSFLLFSSFYPHQIFGNAEKTGDENNSIRGMFVFGSSLVDNGNNNFLQNLAKADYLPYGIDFPLGPSGRFTNGKNIIDLLGDQLKFPAFIPAFTDPSTKGNRTLLGVNFASGGSGILDDTGSIAGEVMSLNEQIKKFEEVTLPEMENQLESSSRESLSKYLFVIGSGGNDYTLNYFFTKSQANISVQAFSANLTSTLFTQLKKLYNLGARKFVLMSLYPLGCSPGTISASAQPQRKGCNQYLNEAAHIFNTNLRSLVNDSRTEMPGSDLVIVNAYKIVRDIIKNPALKGFTDATQPCCHVPSLEEGGNGISCKRGGSTCEDRSKHVYFDGLHPTEAVNVVLATKAFASNSTTEVYPFNIRELAQIQGHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8K5</accession>
    <name>A0AAF0X8K5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0623161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056699">
        <property type="entry name" value="DUF7797"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47527">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47527:SF3">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25073">
        <property type="entry name" value="DUF7797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="374"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="743"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH04169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="871" mass="94578" checksum="25E046B2D8ED97C1" modified="2024-05-29" version="1">MDSIGVAEASGQSVRSEGSKRLSENEELDVRLVKKAKNGGELGDRVRKVAEIVLVLATMGKIRGGEVPSGVEMEMMEDARARLVEVSEQFAPKDVFPTQVFGSIIEDLGLDCSRERKLGFQPPKVSIGQRVNITKQKMENSEKYALQSANYISQAAESNIRSSVQIHSGVQTFSLEKTSPGPSSLGLQPVSASGPVSAANSTSLPYQLPTSEVRPSVSSGLPSRNIGKHNSALALARADRPFYTLTGKSNGVSYTSQVQANSFGDNTKPPTWTVQSQASPSAKTVLDNNASFHASIKGGATGMSTMSRPFMNQTTSVNPLGAHQHIQQGARFVQSPSQSNSHDDIGKIVQKLLQQQILKHPIWTPPSRDYMNKSVPCQICKLVISEVDNVLVCDACEKGYHLNCLQMLNPKSLPKAEWQCGKCFSLSGGKPLPPKYGRVTRNSTVLKVSSPATEVHLSADQNVGVIDVKDKLKRTAINVNSGIQSAPPGALDKSNHSLATAQMGDGKIMRGNGATSTEGECVEEHPSRCSPNNLMRIPGVPIGTSNHFAIDAELADLKVMQRNGSLSSNGKKAGEHPSEPVANNLVVTLGASNVTHDDLKVERLTEEKLDPKCNSQPSAECVVVGSTFDDSQTLGHNKDNVQTELTNRSAIPSKQCPDTNTKMLELGKSCGKDFVEHKTHDNADKDNHGVSVLNHVETTGTGIGPEERGESLSDCLHDVDWIGDKIQVMDGKTYYQSYSTSGVLYKVQDHALFRINNNVSAPFKLQGMWEDSKTRSKWVIASRCYFPADLPEGVGRPCSPEMNEVYESNHDTILSAGLSEGLCKVLPSRTFTEETERRTRFEMEGSDNLCPLYICKWFYDEKKGLFRAVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF84</accession>
    <name>A0AAF0XF84_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase TIC 32, chloroplastic-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0624665</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF84"/>
    <dbReference type="KEGG" id="dcr:108226541"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF198">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH05251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36570" checksum="0D746A2F2377D28D" modified="2024-05-29" version="1">MINDTLKYLAGSAGPSGYGSKSTADQVTEDFASCFLPCSRNLTAIITGATSGIGEETARILAKRGVKIVMPARDLKKASEVKEKIQKESPEAEIILMEIDLSSFSSIKRFCTEFLALRLPLNILINNAGKFSNKLEFSEDKIEMTFATNYLGHFLLTELLMEKMVETAAEKGIQGRIVNVSSVVHGWVKREHFCFNQMLQPKKYNSTRAYAQSKLANILHAKEVSRQLMARKANVTANAVHPGIVKTGIIRDHKGCFTDSLFFVASKFLKSTSQGASTTCYAALSPQMEGVSGKYLTDCNETHCSGLADDESEAQKLWMQSCALIQRRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHZ0</accession>
    <name>A0AAF0XHZ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Apoptosis-antagonizing transcription factor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0726794</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHZ0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="4">
        <location>
            <begin position="90"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="3">
        <location>
            <begin position="291"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH07364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="42872" checksum="5BB0A8FF83A8EF91" modified="2024-05-29" version="1">MKRGREADCEDDSDGDEDNEDDGFKDDDSDGDEDDEISGEEEVEDDEEMENAKNEERNIAEVEELEKEYMELRHEEQDLLKNLRRQKDEDVLKGQAVKNQKALWDKTLELRFLLQKAFSNSNRLPQDPIRSSLCDSSSETSEAYSDLITTSKKTLDSIQKLQEALLEKNPTITQGADGTSGKNTKQLKASENLMVEEDQEWSKISEMQSRIVSFRNKAVDKWQRRTQVTTGAAAIKGKLQAFNQSISDQVSAYMRDPSRMVNGMQQRRSAIAIFGTEVNGDPELLDDSEFYQQLLKEFFETIDPTSSEAAFYALKRMQTKKRKVVDQRASKSRKIRYHVHEKIVNFMAPRPMNLPPMAPKLFENLFGLKVQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIP8</accession>
    <name>A0AAF0XIP8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0727291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIP8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09074">
        <property type="entry name" value="INPP5c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000011">
        <property type="entry name" value="Type II inositol polyphosphate 5-phosphatase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056454">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056455">
        <property type="entry name" value="Ig-like_IP5PC_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF261">
        <property type="entry name" value="TYPE I INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23754">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23755">
        <property type="entry name" value="Ig-like_IP5PC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="11">
        <location>
            <begin position="575"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH07857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1107" mass="121962" checksum="FA64D8FFC366E1CB" modified="2024-05-29" version="1">MCVLGNTGEENVNNKNNSNNDDASESSLPLPLPPGRSISSQTPGEQSKQQLQNDENNSALKNTRKTQSHQLRTNTGTRIKHNRPQLQHNHSLDEHNQGKSDSEEEQVKSSYEYITPPDGKSLPEFIGNAENAPGILKAPRRGAVHPDRPTFLELRPHPLRETQVGKFLRTIASTDTQLWAGQECGVRVWKLPDVFEPGIGIGGRARRGDEEAAPFYESVSTSPAMCLAIDSGTRLVWSGHKDGKIRSWKMDEELNDDPFPEGFSWQAYRAPVLALVISSYGDLWSGSEGGAIHVWPWEAIDKSLSLKMEERHMASLLVERSGISLRSKVTLNGVCNLSSQDVKILLCDKVVAKVWAFGTSSISLWDARTKELLKVYNVDGNIENRADMPLGQDQRVDDETNPKLASKSKKEKPHGFLKRSRNAIRGAAGVVRKATTKSVTEESKLKIEAAVLASNGMIYSGCTNGLIIQWDGNGNRLQEFNHRPCAVLCFCTYGSRIWVGYVSGIVQVLDLDGNLIAGWVAHNGPVLKLVVGRGHFFSLATHGGIRGWSTVSPGPIDQIIRPELSKKELSYTRQENVKILVGTWNVGQGKASHDSLIAWVGTAVSDVGIIVVGLQEVEMGAGFLAMSAAKETVGLEGSSIGNWWQDAIGKALDEGSTFERVGSRQLAGLLIAIWVRTSIRTFVGDLDAGAVACGLGRTIGNKGGVGLRLRVYDRIMCFVNCHFAAHLEAVSRRNADFSHIYRSMAFSRSSNLLSNASAGVSSTAQGVRGTETINPAKGKPDLAEADLVIFCGDLNYRLFGISYDDARDLVSQRSFDWLRERDQLRAEMKAGKVFQGMREAIIRFPPTYKFDIGKPGLGGYDSGEKKRIPAWCDRVLYRDSRSSTSLECSLEFPVVASILQYEACMDVLESDHKPVRCILNVNIANVDRSIRRQETGKIIKSHETIKSLRHELCGIPETEISTTHISLQSQETGSFKITNKSATEKAIFQFICEGQANIKMDDEQELVYRPRGASGFPRWLEVIPARGVIGPDQVADILVRHKDLNATEQLVEGVQTWWSEDKCDKEALLKIIVTGNYSLDTRSHTVHVRHCYSSKGTQTDSSPKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJQ6</accession>
    <name>A0AAF0XJQ6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0728739</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJQ6"/>
    <dbReference type="KEGG" id="dcr:108196480"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="78"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH09283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="49546" checksum="29F1D73E57C03E5B" modified="2024-05-29" version="1">MKCFPYLKEKIRSKQQRSAPELKTQNHSSTSNVSTSGRVTQSSSSTISSRGIPELFEEKAHNLRIFSYSELRRATNDFSRLLKIGEGGFGVVYKGTIKAVDGRGDRIVVAIKKLKQDGFQGHKEWISEVLFLSVVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNKSLGDHMFNKAYVALSWDKRLKIVLGAAQGLAYLHEELEVQVIYRDFKSSNVLLDEDFNPKLSDFGLAREGPTEGNTHVSTAVMGTHGYAAPDYIETGHLTSKSDVWSFGVVLYEILTGRQSFERNRPRAEQKLLDWVKKFPFDSKKFDLIMDPRLESQYSSPAARKIAKLADSCLMKSAKDRPTMSRVVEILKHIIQVSGEYDSSTETFVFSDDESVEQNKSPEEERVSKSAKRRMLHLATLGDHVNGLSKRGFMIMQRTKVPQKIASLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKY8</accession>
    <name>A0AAF0XKY8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0728393</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in recombination ability and DNA strand transfer activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKY8"/>
    <dbReference type="KEGG" id="dcr:108195899"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000087">
        <property type="entry name" value="Recombinase RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049261">
        <property type="entry name" value="RecA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049428">
        <property type="entry name" value="RecA-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900:SF6">
        <property type="entry name" value="DNA REPAIR PROTEIN RECA HOMOLOG 3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="RECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21096">
        <property type="entry name" value="RecA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="RecA protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="89"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="10">
        <location>
            <begin position="257"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH08942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="416" mass="44730" checksum="FE77CF9A331938A2" modified="2024-05-29" version="1">MARLFRSAALLKKSLLSPQGLREGLLGTSSQIRNLSSKGKRKCKSELSDSGEENLSKKDLALKQALDQITTAFGKGSIMWLGRSASPKEVPVVSTGSLALDIALGIGGFPKGRVVEIYGPEASGKTTLALHVIAEAQKQGGYCVFIDAEHALDPILAKSIGVNTENLLLSQPDCGEQALSLVDTIIRSGSVDVVVVDSVAALVPKGELDGEMGDAHMAMQARLMSQALRKLSHSLSLSQTILIFINQVRAKLSITGFGGPTEVTCGGNALKFYASMRLNVRRIGFVKKGEEIIGSQVLVKIVKNKHAPPFRTAQFELEYGKGICQEAEVIELGCKLKFITKSGSFYNLNGKSYHGKDALKRYLAENVSVKDELMANLREKLTENEPDKDTEIHTEVAGDLTVEVASDEELATAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XL45</accession>
    <name>A0AAF0XL45_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Obg domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0727856</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XL45"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000515">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="entry name" value="GTP-binding protein OBG, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008954">
        <property type="entry name" value="PRK12296.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008955">
        <property type="entry name" value="PRK12297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008956">
        <property type="entry name" value="PRK12299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF44">
        <property type="entry name" value="GTP-BINDING PROTEIN OBGC, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="Obg GTP-binding protein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="10">
        <location>
            <begin position="174"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="337"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="9">
        <location>
            <begin position="532"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH08416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="68996" checksum="4D46C4059171E648" modified="2024-05-29" version="1">MASTSSISIYTPFSRQLLSPPYCKIKRSPRKAKPRKLTPNPNPLLKSRSIPQLEATTYTRLPPKDDFIADPSQFSAPEITLSDLNSIRDVSARKPPVNGEKVEEIGGGDEELGFDYGKFELFEIGSDNEEDDEEFDDEVVLGFGDGELGDEFEGVDENGEEGEREKEKGVPAVMRCFDRAKIFVKAGDGGNGCVAIRREKFVPLGGPSGGDGGRGGHVYVEVDGSMNSLLPFRQSIHYRAGRGSHGKGSNMHGAKGDSVVVKVPPGTVVREAGKDGEEGELLFELLNPGQRALLLPGGRGGRGNAAFKSGTNRVPKIAENGEEGAEMWLELELKLVADVGIVGVPNAGKSTLLSVVSAAKPNIANYPFTTLLPNLGVVSFDYDASMVVADLPGLLEGAHRGFGLGHEFLRHTERCSVLVHIVDGSSQQPEYEYDAVRLELEMFSPELAEKPYIVAYNKMDLPDAYENWPSFKETLQSRGIEPFCMSAATGEGTREVIYAAYELVQKRKEAAKGEGFNSPLNLNHVADMVSKQRKSPINEFEISHDTSSNTWNVVGAGLQRFVQMTNWSYVDSDRRFQHVLEACGVFKSLTRLGVKEGDTVVIGEMEMVWHDYKNGGSSNIKRRAAAVRWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XM00</accession>
    <name>A0AAF0XM00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 22">Pantothenate kinase 2</fullName>
            <ecNumber evidence="5 22">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0730017</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the phosphorylation of pantothenate the first step in CoA biosynthesis. May play a role in the physiological regulation of the intracellular CoA concentration. Functionally redudant with PANK1. The phosphatase activity shows preference for normal or oxidatively damaged intermediates of 4'-phosphopantetheine, which provides strong indirect evidence that the phosphatase activity pre-empts damage in the CoA pathway. Hydrolyzing excess 4'-phosphopantetheine could constitute a directed overflow mechanism to prevent its oxidation to the S-sulfonate, sulfonate, or other forms. Hydrolyzing 4'-phosphopantetheine sulfonate or S-sulfonate would forestall their conversion to inactive forms of CoA and acyl carrier protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-4'-phosphopantetheine + H2O = (R)-pantetheine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16753"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:68329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-4'-phosphopantetheine sulfonate + H2O = (R)-pantetheine sulfonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68336"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:177300"/>
            <dbReference type="ChEBI" id="CHEBI:177301"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:68337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-4'-phosphopantothenate + H2O = (R)-pantothenate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68332"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:68333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:16374"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 22">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the C-terminal section; belongs to the damage-control phosphatase family. Phosphopantetheine phosphatase II subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="5 22"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XM00"/>
    <dbReference type="KEGG" id="dcr:108195871"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000442">
        <property type="entry name" value="Pantothenate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1700.10:FF:000002">
        <property type="entry name" value="Pantothenate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000273">
        <property type="entry name" value="Pantothenate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000003">
        <property type="entry name" value="Pantothenate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1700.10">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035073">
        <property type="entry name" value="At2g17340_3_helix_bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015844">
        <property type="entry name" value="PanK_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036939">
        <property type="entry name" value="PanK_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 22" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0533">Nickel</keyword>
    <keyword evidence="9 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="24">
        <location>
            <begin position="593"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00068274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR036939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH10548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="917" mass="100772" checksum="E22F42DB5EF029B0" modified="2024-05-29" version="1">MAAAPTDRQPLLVNSDVISNDDNNNCNSASISTSSDITSSLSGGGDKEMAPVPGSSIHRSGSRPQLDLSGAAIQGNFEEKDPTILLPNQSDDISHLALDIGGSLIKLVYFSRHEDRLINNRRTKSVKEKFGLPTGIRRSYPILGGRLHFVKFETHKINECLDFINSKQLHRGGMDSLGWQSEVESNENAVIKATGGGAYRFADLFKEKLGVTIDKEDEMNCLVAGANFLLKAIRHEAFTHMEGHKEFVQIDQNDLFPYLLVNIGSGVSIIKVDGDGKFQRVSGTNVGGGTYWGLGRLLTKCKSFDELLELSQRGDNSNIDMLVGDIYGGLDYSKIGLSASTIASSFGKTISENKELGDYRPEDISLSLLRMISYNIGQIAYLNALRFGLKRIFFGGFFIRGHAYTMDTISFAVQFWSKGEAKAMFLRHEGFLGALGAFMSYEKHGLDDLMVHQLVERFPMGAPYTGGKIHGPPLGDLNEKISWMEKFVQKGTEITAPVPMAPPGTTGLGGFEVPSSKGDSLRSDASNLNVGVLHLVPTLEVFPLLADPKTYEPNTIDLLDRGELDYWFTVLSAHLPDLVDKAVASEGGTEDAKRRGDAFARAFSAHLARLMEEPAAYGKLGLANLLELREECLREFYFVDAYRSIKQRENEASLAVLPDLLLELDGMDEERRLLMLIEGVLAANIFDWGSRACVDLYHKGTIIEIYRMSRDKMHRPWRVDDFDVFKERMLGSGDKKPHPHKRALLFVDNSGADIVLGMLPLAREFLRRGTEVVLVANSLPALNDVTAMELPEIVAEASKSCDILRRAAVAGGLLVDAMISSQDSSVDNSSSVPLMVVENGCGSPCIDLRQVSSELAAAAKDADLIILEGMGRSLHTNYNAKFKCEALKLAMVKNQRLAEKLIKGNIYDCVCRYEPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XP76</accession>
    <name>A0AAF0XP76_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0730131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR052957">
        <property type="entry name" value="Auxin_embryo_med"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387:SF3">
        <property type="entry name" value="ATP_DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387">
        <property type="entry name" value="WU:FJ29H11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH10662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="167" mass="18713" checksum="C62B91324C9548F5" modified="2024-05-29" version="1">MSIAASSNLSRDNVLSILKFIKFLRLKLLSPAEFINSIKGGRWLRTNQGDRSPADNIPLIDEVYYGANLMSYKVELGLIGVRVNFDGNYQLVGDNLKSSNCLSSLFADALYLILNCLRHLRSTNNLVHALKDKKCIKTDDGFTSPTEFYCLTLSGDVFYRFSIASRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRJ3</accession>
    <name>A0AAF0XRJ3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832450</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16083">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH12941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="622" mass="69461" checksum="F29C9450E266C6C9" modified="2024-05-29" version="1">MRFSIDLTTTPDFTRLPRLENLYLEGCRSLKEVHISIGSLVNLVSLNLKGCLNLRSLPDTICELKALEVLCIDGCTRLEALPTQLGNVKSLIELNACWLRVLELPDSIGDLTKLVKLKLDYNEKLEYLPNTICNLRALEVLSIMYCPKVEALPVELGNIESLIELRAGGTTVSKLPNTICNMRLLEILDISDCYKLDSLPDQLWKLTNLSIADLSGCGNLLPRLENLYLEGCTSLKEVHISIGSLVNLVSLNLKSCSSLRSLPDTICELKALEVLCVDACIWLKALPTQLGNLKSLLELNARWLRVLQLPDSIGDLSKLVKLKLDYNENLEYLPNTICNLRTLEVLSIMYCTKVEALPVELGNIESLIELRAKGTTFSKLSNTICNLRLLKNLDISDCYKLDSLPDQLWKLTNLSIADLSGCGNLLSIADLPPYLTRIDARSCLSLESVNVSNLKLLKELVLTDCRALTKILGLGELASLYRLQLRGCHSSLLARTLTMQLFELMGDLAYDISSSVNLQANLSYSYLGMILCFTPEIQEVFSAKEVYYTVTADDQSVGQKRLYGSYIVIIPRSVFTLAKDNHTIKVTSNAGRVWFYLLYKNEDDNTLVSQRFQVEENPGTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XT06</accession>
    <name>A0AAF0XT06_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable 6-phosphogluconolactonase</fullName>
            <ecNumber evidence="5">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0832945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XT06"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF17">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="268" mass="28985" checksum="3B15B006F71C7368" modified="2024-05-29" version="1">MAFSGDGKDGRELRVYENLDELKVDLVEYIAKLSDAAVKERGTFAIALSGGSVIGLIGALCQAPYRETVDWSVWQVFWADERLVAKDHPDSNYKLAKDGLFSKLKVLVLPSQLHSVNGLLSAEDAAVDYEFVIRQLVRTRLIGVSETSDCPKFDLVLLDMGSDGHVASLFPGHFDPDETEEWVTYITDSPEPPSGRITFTMPVINAAANVAVIATGSDKAEAAHLVIDDAGPECPLPAKMVNPADGKLTWFLDKLAASKLGSCAQSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTY5</accession>
    <name>A0AAF0XTY5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0832603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTY5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF94">
        <property type="entry name" value="ALPHA-FARNESENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="3">
        <location>
            <begin position="238"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="61599" checksum="3DECA67ACDFFE954" modified="2024-05-29" version="1">MIAETDQIQKTGVYRPNIWKYDRIEALRNEYADEKYERMLGKLIGEVQGLLSNTADTWEKLELLDNMEKLGLTYLFEHEIKKVLDTLVSNNNLNLDGEKDLYNTALFFTVLRKHGFHVSQDMFADLVDNTHSDMKAMLKLFEASHLAFEGENILDRANLISRNYLKSIGSSAEDASLANVTRSLKDPYNIWYNVKTQIRFHGKNTNSSSHLLNLARYNFNMIQATHQKEVKELLRWWKALDLKRSLPFIRNRVMESFVCAVGIVSEPKYGSLRNWLTKSITLILVLDDVFDASGCTLEELENFTSALNRWDGEQNEKLSAGMNICLQTLYDTIKNIASEIEDVYGWNSVSPHLQKAWTTFCQSMLKEARWFNTKYTPSLKEHTDNGRNSSSGPLLSLHIFFALVTKTEPNQVIELLQCTAKHQHNVALIFRLCNDLGNYAVEIERSDASSSIQCIMQEQGVSEEVARDQIKHMIANAWKKINYQCQTQSPLLQPYLKYSANIARVAHVVYHNGDGVSNADGMTRNQVMDLLSEPLLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYI5</accession>
    <name>A0AAF0XYI5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ORF">DCAR_0935328</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYI5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="9">
        <location>
            <begin position="31"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH15782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="228" mass="26456" checksum="4C3A16B0669F0E9A" modified="2024-05-29" version="1">MITSHAGNDGTNKTVENCIDVDEPEEKQTRKRTSEVWKDFTKVKNSNGIEKAICDHCKKSFVGDSTSGTSHLAMHLKRCTAKIYKIRDRCQDKSRQDLAKMVVKHNYPFNMAKHEFFENFCSGLNPDFRLHSRTTVRSDVIKLDEEIKGHAHYIWTSDSQNIAYAALTVHYIDSEWMLSKKILNYRYISYPHDGESLFRFISQLIMEWNLDKKLFSMVVDNASSNDCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XYT3</accession>
    <name>A0AAF0XYT3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0936018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XYT3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
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        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="288"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="368"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH16465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="448" mass="47467" checksum="87C7132513371BCF" modified="2024-05-29" version="1">MAQFQALNQVLQNPSSGAYFEMVQKRKKLPMSLQETLNKAFANIPVSTFPIVPGGKVLEIQANASVGEAIKILSDANILAAPVCESDDVKAVDWRERYLGTIDFSAIVLWVLESADIAAAALSASSAAAAGVGAGAAGALGALAAGGTGPVAAAGIAAAAVGAALAGGLAGDQVGKDASSAADTLGDDFYKVLLEEEPFASTTVRSIITSYRWAPFIPVAADSSMLGVLLLLSKYRLRNVPIIEDGKPTIKNFITQSAVVKGLDNCQGRDWFDCIAAQPITELGLPFMSNDEVISIKSSELVLEAFKLMKDNQIGGLPVIEGPEKNIIGSLSIKDIRFLLLNRELFAKFRELTVKDFMNTIASEARDLGKGTIPATCSLSSNLGSVIHILASKMVHRVYVVGANGKEVVGVITLRDIISCFIFEPPNYFENYLGLTSYEVKIDAFKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALI3</accession>
    <name>A0AAF1ALI3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0104102</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202429"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="187-kDa microtubule-associated protein AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2700">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056284">
        <property type="entry name" value="AIR9-like_A9"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056287">
        <property type="entry name" value="PH_AIR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149:SF11">
        <property type="entry name" value="187-KDA MICROTUBULE-ASSOCIATED PROTEIN AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23197">
        <property type="entry name" value="IG_AIR9"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23218">
        <property type="entry name" value="PH_AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="788"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG84917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1712" mass="187784" checksum="227E798632F5F775" modified="2024-05-29" version="1">MEDPLVAGEGSVELSSNAKQKLPKLTENAKRTTHIAKPTVTGPIGTAKKRTELKGGSELRSNVAKPTTGKAKLTATSGGLSSVPVTRRKSTGGLPEKQPVSASKRQNNASSAVGGKPTSASSSEPLRRSLPEVRRSSLSSIVAKPSTRPSVSDTRNAISSSQVSRTSRSLLNSDVGKRDATKRSALNSSTSLSTSQMSLDSTGSSTFRRAVSKVSSPSSRSPSISSGPKISSLSTSRDRNSNLSDRKKSTRADSRDSNFIRLPQVELKAGDDVRLDLRGHRIRSLNANGLNLSPELEFVYLRDNLLSTLEGIEILKRVKVLDLSFNDFKGPGFEPLENCKALQQLYLAGNQITSLVSLPELPNLEFLSVAQNKLKSLSMASQPRLQVLAASKNRLTTLKGFPHLPALEHLRVEENPILKMSHLEAACILLVGPTLKKFNDRDLSRDEKAIAKRYPAHSSFCIREGWEFSRPEHAVDSTFSFLVKKWEDQFPPGYLLEEASVDQPFEEDACGCHFQFVKDKNVNTDSELVLKYQWLIGDRTPFNFKAIPEATGEVFWPKHEDVNKILKVECTPILGDIQYPTIFAISLPVAPGTGYPKVLKIDVRGELVEGNIIRGYPEVAWCGGTPGKGVASWLRRKWNSSPVVIVGAEDDEYQLTLDDIGSCLVYMYTPVTEEGSKGEPQYAITDYVKAAPPSVDDVRIIGDVVEGTTIRGVGDYFGGREGPSKFEWLRENKDTGEFELFLTGTTEYTLTKEDVGRRLAFVYIPTNFEGQEGKPLTTTSQVVKQAPPKATNLKIVGDLREGSKVTVTCNVTGGAEGSSRVQWFKSSCAIFEGEKGLEAVSTSKIAKAFRIPLGAVGYYIVAKFTPMTVDGESGEPAYTIAEKAVETLPPSLNFLSITGDYTEGGILTASYGYIGGHEGKSIYSWYIHEIDTDSGTLIPEASGSLQYRLSKDAIGKFVSFTCTPIRDDLLVGEPKTCMGQERVRPGSPRLLSLQITGFAVEGNLLRVDKKYWGGNEGESIFRWFRTSLDGTQTEIKDVATSSYMLSGDDIGYSISVSCEPVRVDWARGPTVLSELTGPVVPGPPTCLSLAFQGSVVEGARLTFIASYSGGEKGDCQFEWFRVHYNGSIKEKLIAGEYLDLTLEDVGSSVELVYTPVRKDGVKGSPVSIVSTPVTPADPVGMDLVIPNCCQNEMVVPQKTYFGGQEGEGKFIWYRSRIKLEKSAFVDISNVHEDLVICGSSLEYIPSLEDVDAYLALYWLPTRADGISGSPLVSVTNLPVSPALPVVSDVHVRKVSSTTYSGQGVYFGGYEGESLFSWYRESDDGKIVVTSGVDCKTYEVTDADYSYRLLFGYTPIRSDSLVGELRLSEPTEVILPEPPIIEMLALAGKAVEGDVLTAVEVIPKSETQQRVWGKYKKEIRYQWFYTSETENINSFEQMPSQRSCSYRLRFEDIGRQLRCECIVSDVFGRSSEPAYVNTATISPGFPKIDKLEIEGRGFHTNLYAVRGTYSGGKEGKSRIQWLRSMAGSPDLISIPGETGRMYEANVDDVGYRLVAIYTPVREDGIEGQPVSASTDSIAVEPDVLKDVKQKLESPSSVKFETLCDKDRSSKKVSSVGTLERRILEVGKKKLKVVKPGSKTSFPNTEVRGSYAPPFHVEVFRNDQHRLKVVVDGENEVDLMVQTRHMRDVIVLVIRGLAQRFNSTSLNSLLKIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMB2</accession>
    <name>A0AAF1AMB2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0207704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6376" checksum="EF788D895B756D90" modified="2024-05-29" version="1">MPLKRLVEVEPPSPLRYLIGAAIMMIGVVLPVGYMMFRNKRVPSSSSFAKQTNKVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="7">Tetratricopeptide repeat protein 27 homolog</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
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    </dbReference>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="735"/>
            <end position="767"/>
        </location>
    </feature>
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        <location>
            <begin position="741"/>
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        </location>
    </feature>
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        <location>
            <begin position="752"/>
            <end position="766"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG90974.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUN4</accession>
    <name>A0AAF1AUN4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
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        <name evidence="2" type="ORF">DCAR_0414934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="203"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="335" mass="38253" checksum="1CF3263E4A44E21D" modified="2024-05-29" version="1">MFEEASGQQVNLSKSSVIFSSNVDRESRETVCNVLHMQEAGDDVTYIGLPNMIGMNKLKVLGFLKDRMNNKVQSWKESWISQAGKAILIKNVAQAVPNYAMSTFLLPLEITRDFERSLSKYWWEILNQPWLNNVSNAFVTTEVQGLDNATVDQLMVTEGGQWDIDIVEDLLNVKDKQCILNTMVGGDREEDVLYWNEDLSGDYTVKSAYQMLQRQKGLWSTADNSNLWKVIWSIKAPPKVLNMVWRALSNSLPTREVLAEKRVPLTLICPFCTGNGESILHVLVQCPFADQCWRKRGRGYQPLAEVSFDRWLQSTKTLYRDVNIRDGVCSWAKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW52</accession>
    <name>A0AAF1AW52_DAUCS</name>
    <protein>
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            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0313917</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW52"/>
    <dbReference type="KEGG" id="dcr:108213325"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF245">
        <property type="entry name" value="(-)-ALPHA-TERPINEOL SYNTHASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="7">
        <location>
            <begin position="23"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="8">
        <location>
            <begin position="255"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG94621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="551" mass="64035" checksum="7CAA756377B80E5C" modified="2024-05-29" version="1">MSTLIAKHQLPVIRRSANYQPCIWTNDFVQSLTTDYTGERFNGRVNELKENVIEMLNDVAKPNLDQLELIDNLQRLGLGYHFEHEIKSILMKIYEDDQSSETLERNDLHAAALKFRLLRQHGYDISQDVFKYFMENGSFKGCLCNCFKGVLSLYEAYYFSSEGESIMEDAWSFTSKILKENLDDIIDPNLGINVRHALELPLNWRMPRLEARWYIDFYGRINNMNPALLELAKLDYNTVQGLYQEELKIASRWWKELRLSTKVSFARDKLVESFIWMVGIIPEPQFAYCRIEVAKAIQMTSVIDDIYDIYGTLHELELFTDAIERWDIHSIQLLPDYMRIFFLALYNFVNGIVYHIFREQNIDVLPHLKKVYTDLVKCYLVEARWYHSGYTPTLEEYLDNGKLSIAQPSVVIQSYICSANPIEKEKLECLLAMPYILQLTGTLCRIVDDYGTSSDEIARGDVPKSIQCHMFHNGTSEEAARAEMRDLMRKKWREINACRARDMPVSRSSVEIMLNASRSAHYIYYDSDGYAGKDGKGIDMILSLIVQPVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B0P0</accession>
    <name>A0AAF1B0P0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0521583</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B0P0"/>
    <dbReference type="KEGG" id="dcr:108223605"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000005">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000058">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000001">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF62">
        <property type="entry name" value="OS05G0423500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041955343" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="27"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="492"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH02194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="834" mass="92206" checksum="B9D64DFACACD7A9E" modified="2024-05-29" version="1">MMDSCMFKFFIWGSCILFYLACFSWGFDPADQYYINCGSSTSVTIGSQVFLGDKLASKYLSSPDNILGTTSSNSIPQSDESGLYKNARIFTKESKYTFPISRKGRVWIRLYLSPFVYDSYNPNSAAFSVSIPNHMLLSNFKASEAVRKEFLVNVTSGSLVITLSPSENSFAFLNALEVVSAPDILISDDAQSVNPAVKFNGLLTQALETVARVNMGGPTVTSKNDTLGRTWVPDTKFLLEQNLAESVSKINSVRYPPGGATDDIAPKTVYGTATKMKSEDNPNSNFNVTWVFPVDPGFRYVVRFHFCDIVSDAPSKIYFNVFLDSSNVLPDLDLTTRNLNFLATAIYMDFVTSSISSSKLRVSIGPSPVNDAYPDAILNGLEIMKMNNSLGNLGGMATLPSSSGSGSKSKVGVIVGVIIGVAIILLIVGLLFYIRRKRRLEQLHHSKTWVPISQNASYTMGSKDSHKTTASAGSDIGFRCPFAAVQEATNNFDESWVIGVGGFGKVYKGEFSDGTKVAVKRGNSKSQQGFTEFRTEIEMLSQFRHRHLVSLIGYCDEKSEMILIYEYMENGTLKSHLYGSGLPSLGWKERLEICIGSARGLHYLHTGYAKAVIHRDVKSANILLDETFLAKVADFGLSKTGPEIDQTHVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEVLCARPVIDPSLPREMVNLAEWAMKWQKRGQLDQIIDPSLEGKIKPDSLRKFAETAEKCLADYGVDRPSMGDVLWNLEYALQLQEAVLHNDPEENSTNIISDLSPQVNNFSHIDSTTSARQHEMSVTDDDLSDVSMSKVFSQLIKSEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3U3</accession>
    <name>A0AAF1B3U3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0624579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH05165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
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    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="245"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34208" checksum="8BAA29F7329CC04B" modified="2024-05-29" version="1">MDTILVVVDRFSKFGHFMALHHPFTAVEVAELFLDNIFKLHGMPDSIVCDRDKIFTSVFWGSLFEKLGISMHFSTAYHPQSDGQTERVNQCLESYLRCFTGDRPHSWKQWLSLAEYWYNTTYHTSLGSTPFEVLYGDKPVPLNLGELHDMVIPVAQDMLQQRQVVLQVAKESLTKAQHRMKFYADQKRTERMLDKVGTVAYRLKLPSHSRVHPVFHVSLLKKHVGPAPIMEGELPQTNEADIVTLEPSRVLQRRQVQRQGQNITQWLVQWKGLESYEASWEDVSFITNQFPQFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B420</accession>
    <name>A0AAF1B420_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0522628</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH03232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:root hair elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
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    </dbReference>
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        <property type="entry name" value="Actin"/>
    </dbReference>
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        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
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        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41729" checksum="62CCC58C7F5DCC62" modified="2024-05-29" version="1">MAEAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYSLPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDMKEKLAYIALDYEQELETSKTSSAVEKSYELPDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKELYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B4F0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0623662</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4F0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 15" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042018766" description="Receptor-like serine/threonine-protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="18">
        <location>
            <begin position="24"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="287"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="19">
        <location>
            <begin position="342"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="503"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH04253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="819" mass="93156" checksum="563DB0123473180C" modified="2024-05-29" version="1">MTSTAVNFFSTILLLCILINSSATDVLRINQTLRDGHTIVSQHGEFELGFFTRGSSTNRYLGIWFKKISYGTVVWVANRDTPLNNTSGMVRIDNKAILLVSNASNTIIWSSNYSTSVNNPVAQLLDTGNLVVRDEKDGGQENFVWQSFDYPGDTMLPGMKFGKNLVTGQQWYYSSWKSVDDPSTGSFIHRMDTHGYPQLLVWKDSKIQARTGPWVGNRFSGDPVPKANRIFLSEFFIEQREIYYAFHLLNRSSITPVTRLMLDPNGGRQRLIWNYQNQEWTTYRTIQVNDCDRYAPCGPYATCDPNNSPKCACLRGFKPRSSEKWEAADWTDGCVRTNSLDCDNGDGFVKYTGVKLPDTQQSWYNLTMGLKECKSLCLKDCNCTAYSNINVENGGSGCLLWFGGLMDIKVDTEDAQSIYVRMPASDLDESKRSGVRRILIISLTIALTAMSALILFYLFKKRKLKRKGKFCSHLAERLSSNEDENLELPLFDFRKIVKATTNFSNDNKIGEGGFGSVYKGTLEDGQLVAVKRLSDSSRQGIDEFKNEVSLIAKLQHRNLVSLLGYCIEEQERILVYEYMPNNSLDSFIFDEKRRLAGWQRYYSIINGVARGLLYLHQDSKLRIIHRDLKASNVLLDQDMNPKISDFGMARSFGGSQTEANTTRVVGTYGYMSPEYAIDGLFSTKSDVYSFGVLVLEILSGKRNRCFEHPDHNLNLLGHAWRCFNEDKLKELMDETIVKPTDHHEVFRVIQIGLLCVQEYPEDRPEMSSVVLMLNSKIALPNPKKPGFFTERRRNERDYSSSKRILSSSYDYSITAIVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7C9</accession>
    <name>A0AAF1B7C9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0625990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7C9"/>
    <dbReference type="KEGG" id="dcr:108224297"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF19">
        <property type="entry name" value="BET V I_MAJOR LATEX PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0017">Alkaloid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="3">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH06562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="156" mass="17226" checksum="E06051E2A20616BF" modified="2024-05-29" version="1">MFGTVSGEVEVNAPASVVWEVYGSLKLAAVIEKGFTDVLQKIEVVEGDGSVGTILNLVFQPGVAPFASYKEKFTMIDNEKRIKDSIVIEGGYLEMGFDRYFLRAEIIEKDEKSCITKATIEYELREEYAANASLVSIDGPMAMMNIVKTHILSTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDD9</accession>
    <name>A0AAF1BDD9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0933381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDD9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="10091" checksum="92827CFF67E7C02C" modified="2024-05-29" version="1">MAGKCFCGKWLAAKTAWTDANAGRRFISCERCRFFRWLDEPLCERSRAIIPGLLRRINKLEAQQRPEACKRIGFKERIRMFRLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BG88</accession>
    <name>A0AAF1BG88_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BG88"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF15">
        <property type="entry name" value="PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="89479" checksum="6B1CCB4B28F90B28" modified="2024-05-29" version="1">MELSISRSFQHSIFRPSLCPIASEHTYRACVYSYRSSRHRLKCNSKPLRFKLSLEQNCSNCGVESVWEDCRFVEVIAIGSRRDAVLDFCLDSPLLGSALRLWDISLKDSVNVELQQRVLEKDIPPCILDTPSTLQSRSKAIILVAGAAYGSEYRVVLDILKTVKSANGLVFGIILKPFSFEGRRRRNEVIELADKLQEHTNFCIMVDTDTLLEKDALTLDEALKTANNAILTAINAISVLVSDVHRKFLDVPNGTMKELEVSELKKVSYQAFLFVAELSMFKKPTCVLQFLGAYQDAKIGFGSAYNIKTSIMRSLYECPFLAVGVKDLGTVALCLTSSGAVKSNDVQSVLRLFRQTSEFRGAVIMSIVHEPKMEPNLIATTVVNLGYAKDVESVKGGIFSRLVQHFPSIFNLWAKREPQSINTEQCDLPEDGHFSKVIPSPDLYDMPSMTSANDTAEDVDSFEVNTLLRSKDDDKIYMSRGYGSSPGQSKIRNSEGDSSPSSSMSADPSTKGSPIFQRERLLSRHNIGAGYAQDWAIEEAVGNSVSASVLDSLGIFKLPIGVKPSEELKEDRSITFNRAEKEDILDAEMKPQPQVSPIVPWDALTDASYEAVTDLYSNASAILKGNNADVSKKQGNLSARAASMLESERESSKKWSPVVEIKYRGGSYMGRCQGGLPEGKGRLSHGDGSTYDGMWRYGKRSGLGTFCFSNGDVFRGPWRDDLMHGKGWLYFHTGDRWFVNFWKGKANGEGRFYSRNGEIFFGHFKDGWRHGEFLCIDVDGERCLEIWEEGILVSRKHMDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BH72</accession>
    <name>A0AAF1BH72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0933970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BH72"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH14451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="382" mass="42363" checksum="95275E6D5DACCD3D" modified="2024-05-29" version="1">MFQRITTLRQIFSSKFIQSQGARSSYHTSGAAVSSKEYEPFAHQNENLSVDWDNLGFNLMPADYMYMMKCTEGESFTQGHLDRYGSIELSPAACVLNYGQGLFEGTKAFRGENGRLFLFRPEQNAVRMQIGADRMCMPAPSTEQFVDAVKEIALANHRWIPPTRKGSLYIRPLLIGSGPILGVAPAPEYTFLIYASPVGNYFKEGMAPLSLYVEDEYHRASPGGAGCVKAITNYSPVLKAQARAKSIGFSDVLYLDSSSKTIIEEASSSNFFIVKDNVISTPAPNGTFLEGITRKSIIEIARDLGYQVDERSVRVDELDHADEVFCTGTAAGVAAVGSITFRGKRFDYKVDGRLVSQRLYSTLVGIQRGVVQDKWKWLVRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WZG3</accession>
    <name>A0A161WZG3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0205486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WZG3"/>
    <dbReference type="Gramene" id="KZN04125">
        <property type="protein sequence ID" value="KZN04125"/>
        <property type="gene ID" value="DCAR_004962"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="214" mass="23043" checksum="7B45274DDD1D05D1" modified="2016-07-06" version="1">MQCRACAAIGCSTKVEVCSVFFDSTVKQVDGTAACKSLEDTIMEDSAEVASLLDAVESAEDKQASAALACPSKSSAECVNNDEDTSANSVRENPDPPSAISFAKNSQSENELNWELARRQLEVSSNLRKHLVPVERLSMLHCTFNTTVLAKREVILLRTINTVPESYCGTNSEAVASGDAYKTASGTAFENGMVEGCLVEVVVLIKKHGIRLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XK99</accession>
    <name>A0A161XK99_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WRKY19-like zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0209631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XK99"/>
    <dbReference type="Gramene" id="KZN07689">
        <property type="protein sequence ID" value="KZN07689"/>
        <property type="gene ID" value="DCAR_008526"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209388"/>
    <dbReference type="OMA" id="QAIGCTK"/>
    <dbReference type="OrthoDB" id="77038at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056866">
        <property type="entry name" value="Znf_WRKY19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827">
        <property type="entry name" value="EMB|CAB89363.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31827:SF40">
        <property type="entry name" value="F22C12.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24906">
        <property type="entry name" value="Zf_WRKY19"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY19-like zinc finger" evidence="2">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="664" mass="69315" checksum="FE79C3995BF5E8A7" modified="2016-07-06" version="1">MDARFRNQGFVSKPALTTFKNLSNTVHVGGAGTASGCADTTLRLDFPGSSVSAGLSASRGIKRKWSLSGGSLEQQIELPLCLGLGRCSSSSDSKGSSATACTTVSSAKETDEGSSMDLELDFSFHLGNEKISSPKNSVSSNLSAPVLQPKVNLDLSLSFKPSESDISSTHPCISPTQHVVENTLNIGAINHKDEGSASSHWKRRNYVPPSLFLEESLREHKPAQTTPDLSSSVVTTPKSSVTCTSGITQQRSSNTKTCQFQGCGKGARGASGLCIAHGGGRRCQRPGCHKGAEGRTAYCKAHGGGRRCEFLGCTKSAEGRTDYCIAHGGGRRCSQEGCSRAARGKSGLCIRHGGGKRCTRENCTKSAEGLSGLCISHGGGRRCQYPACTKGAQGSTMFCKAHGGGKRCTFEGCTKGAEGSTPYCKGHGGGKRCAFEGGGVCPKSVHGGTLFCVAHGGGKRCAVPECTKSARGRTDYCVRHGGGKRCKVEGCSKSAQGSTDFCKAHGGGKRCSWGHPGSDFGKDDSPCNAFARGKNGLCTSHSGLVQDKRVHGGATVGVMVHDSKMCEMTKGVINTEDTSADYMMMDNTVVPTGCIGSMWKEGSLPIGLGATSSRVVVPEGRVHGGGLIALLSGSSEQMLNANNNFVVGHPSEPGKFYMMPRKWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y8Y3</accession>
    <name>A0A161Y8Y3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0729856</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y8Y3"/>
    <dbReference type="Gramene" id="KZM88919">
        <property type="protein sequence ID" value="KZM88919"/>
        <property type="gene ID" value="DCAR_025994"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193178"/>
    <dbReference type="OMA" id="TNNRVTE"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090406">
        <property type="term" value="C:pollen tube"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000192">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000404">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF680">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043848088" evidence="16">
        <location>
            <begin position="21"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="749"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="785"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="792"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH10388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1122" mass="120831" checksum="9FB81103684B982A" modified="2016-07-06" version="1">MPSALVILVLLFSHALLSCADTGTEIEALNSFRLSLNDPLGVLNGWDPTTRSAPCDWRGVTCFNGRVSELRLPRLQLSGPLTDKIANLRMLRKLSLRSNGFNGTIPSSITKCTLLNSVFLQYNSFSGEISPELSNITNLLIFNVAGNSLSGEFSGELPRSLRVFDVSSNAFSGEIPRSLASLYKVQLINLSYNRFSGSIPASLGQLQKLEYLWLDHNVLEGTIPSAIANCTSLVHFSAEGNSIGGVIPAGIGVLPKLQVVSLAHNNLSGSVPVSMLCNVSVYPPSIRNVQLGFNGFTDIIEPQNGTCSSALQVLDLQQNEIKGVVPVWLMNVSSLTMLDISGNLFFGAVPIQIGNLWRLEELRMANNSLSGLVPAEIKRCGNLRVLDLEGNQFVGEIPDFLSEIKGLKMLSLGGNQFSGAVPSRLGNLTQLESLNLRDNNLNGSLPDEVTSLSNLTVLNLKGNKLSGVISSKIGNLRMLSVLNLSDAGFSGPIPSSIGTLYKLTTLDLSKQNLSGKLPFDIAGLPNLQVISLQENKLSGDVPEGFSSLLGLSYLNLSSNAFSGQIPSTFGFLKSLTVLSLSNNNISGSVPPELGNCSALRILDLGSNSLSGQIPPDLSRLSYLTELDLGKNNLTGEIPDEISECSSLKSLALQSNHLSGNIPSSLSGLLNLTSLDLSANNLSGEIPANLTIIPNLMKLNVSSNNLEGKIPAMLGAQFNQTSFSGNGELCGKPLDKKCEGESSSNRKKRVILLMIVAASGACLAAFCCFYTYSLLKWRKRLKAKAAGEKKSTPTRASSGGRSSSVDNGGPKLVMFNNKITLAETVEATRHFDEENVLSRNQYGLVFKACYADGMVLSIRRLPNVLLDDNMFRKEAEALGRVKHRNLTVLRGYYTGPDLKLLVYDYMPNGNLATLLQEASHQDGHVLNWPMRHLIALGIARGLAFLHTAAMVHGDVKPQNVLFDADFEAHLADFGLNKFTVAPPVEPSTSTSVGTLGYVAPEVILTSETSNESDVYSFGIVLLELLTGKRPVMFGQDEDIVKWVKKQLQRGQISELLEPGLLELDPESSEWEEFLLGVKVGLLCTVPDPSDRPTMADIIFMLEGCRVGADIPSSADPTNHHSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YFE0</accession>
    <name>A0A161YFE0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0625347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallothionein superfamily. Type 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YFE0"/>
    <dbReference type="Gramene" id="KZM91649">
        <property type="protein sequence ID" value="KZM91649"/>
        <property type="gene ID" value="DCAR_020986"/>
    </dbReference>
    <dbReference type="OMA" id="SENDCKC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044671">
        <property type="entry name" value="MT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33357">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33357:SF3">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH05924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="61" mass="6473" checksum="205F30D40D406626" modified="2016-07-06" version="1">MSSTCGNCDCADKTQCVKKGNSYGMDIVVTEKISAETMVMNMSENDCKCGTSCTNCTCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AAA7</accession>
    <name>A0A162AAA7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_014778</name>
        <name evidence="5" type="ORF">DCAR_0416070</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AAA7"/>
    <dbReference type="STRING" id="79200.A0A162AAA7"/>
    <dbReference type="Gramene" id="KZM97860">
        <property type="protein sequence ID" value="KZM97860"/>
        <property type="gene ID" value="DCAR_014778"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217283"/>
    <dbReference type="OMA" id="MRTHYCP"/>
    <dbReference type="OrthoDB" id="1934063at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="368"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM97860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG96734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="458" mass="52577" checksum="BAC263ADA9CF5B83" modified="2016-07-06" version="1">MEETDEQEEFLTLTLAIVADSRFDKKRKRRKREESIEPVSSNEDCEGRIFSLLQLREQMLKLDRKRKGVAEDGKGLHLIHLLLISATFVDENKLDSSVESLSELFQNVCLTGDSVQRVAAYFADGLVARLLTRKSPFYNMIMKEPSAEEEFLAFTELYKVSPYYQFAHFTANQAIIEAFEKEEDSNNRALHVIDFDVSYGFQWPSLIQSLSDKATSSSRISLRITGCGRSLEELQETETRLVCFAKGFRNITFEFQGLLRGSSDFADIKRRNNETVAVNLVFHLNTLTNFSKISDTLKTVHFLNPAIVILVEQEASRNPRSFLAQFMESLHYFAAMFDSLDDCLPLDSDGRLGIEKNHLGREIKSFMNYEKDSSNCNKYERMKTWKERMEGHGFRGLKLSSKSVMQAKLLLKMRSQYCPVQCDGENGGFRVFERDESTALSLGWQDRFLLTASAWHCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164T807</accession>
    <name>A0A164T807_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_024310</name>
        <name evidence="25" type="ORF">DCAR_0728095</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164T807"/>
    <dbReference type="Gramene" id="KZM87176">
        <property type="protein sequence ID" value="KZM87176"/>
        <property type="gene ID" value="DCAR_024310"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196460"/>
    <dbReference type="OMA" id="PLKDDTR"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000417">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000430">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000219">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000588">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000680">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At2g33170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF65">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE SERINE_THREONINE_TYROSINE-PROTEIN KINASE SOBIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853287" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="30"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="808"/>
            <end position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KZM87176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH08651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1110" mass="121820" checksum="AA0F5D3C705032C1" modified="2016-07-06" version="1">MYVSVGSRASFLVSLTVIALALLSCPSEGLNAEGMSLLELKKELQDDFNHLWNWNSSDETPCLWRGVSCTSDVVPVVDSLVLRGMNLSGNLSSSIGGLAFLTYLDIAFNAFNGSIPKEIGNCSRLNTIYLNNNQFQGQIPAEICKLSLLTDLNICNNKLSGSFPQEIGNLSLLTNFVAYSNKLSGPLPRSFGKLRSLKTFRAGQNSISGNLPAEIGDCQNLQYLGLAQNLLDGDIPKVLGKLKNLTDIILWGNDFSGFIPAELSNCTNLLTLALYQNNLVGEIPKEILGMKSLWKLYLYRNGLNGTIPREIGNLSSALEIDFSENSLTGEIPVEFSQIKGLSLLYLFENQLTGVIPDEFGSLKNLTNLDLSINRLTGPIPYSFQYLPNMRQLQLFDNSLTGSIPRLLGFYNKLWVADFSDNYLTGKIPPYICRQSILDTLNVESNQLYGNIPEGLLYCPSLSQLRLNGNRLTGNFPSELCNIVRMSAIELSQNKFSGSIPPKIGNCQQLQRLDLSGNNFTSKLPKEIGNLDKLVKFNISSNFMTGKIPPEILNCKTLQRLDLSRNNFEGAIPNELGSLSQLELLKLSDNKFSGNLPAALGNLSRLLELQMGGNFISGEIPNELGFLSGLQIALNLSFNSLSGGIPSELGNLILLEYLLLNNNHLSGEIPTTFANLSSLLGCDFSNNNLSGPLPSVPVFQNMDENSFTGNRGLCGGPLGDCNTKTSFDSVQPLPDGGAHRRKIIAVVAAAVGGVSLILIVVLIYMMKRLPVEIAATLQEKDTSTPVSDIYFPPKEGFTFQDLVEATNNFDDSYVVGKGAAGTVYKAVMQSGQMIAVKKLESNREGNNIDNSFRAEILTLGKIRHRNIVKLFGFCYHQGSNLLLYEYLARGSLGELLHGSSFTLEWPIRFTVALGAAQGLSYLHHDCKPRIIHRDIKSNNILLDDDFEAHVGDFGLAKVIDMPQSKSMSAVAGSYGYIAPEYAYTMKVTEKCDIYSYGVVLLELLTGKAPVQPIDQGGDLVTCVKKYIRNHSLTSGILDSRLNLEDKQIVNHMLTVLKIALLCTSMNPYDRPSMREVVQMLIESNRREGSFITPPIYDSSLEDDVPLKDDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TE65</accession>
    <name>A0A164TE65_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_024542</name>
        <name evidence="3" type="ORF">DCAR_0727839</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TE65"/>
    <dbReference type="Gramene" id="KZM87408">
        <property type="protein sequence ID" value="KZM87408"/>
        <property type="gene ID" value="DCAR_024542"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM87408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="112" mass="12457" checksum="959C19988FFFF408" modified="2016-07-06" version="1">MRLSKNGMSGCGSTIQIMIQQLNQRKSDPNLTLILRPVEASQNALARYLAEIGPLQRYRLVMSMKTGYRQVDEEEVQGEGVIEITDDDEDEDVADASRLVAGADHPGMRGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XZA3</accession>
    <name>A0A164XZA3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0519467</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XZA3"/>
    <dbReference type="Gramene" id="KZM93765">
        <property type="protein sequence ID" value="KZM93765"/>
        <property type="gene ID" value="DCAR_017010"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224006"/>
    <dbReference type="OMA" id="WFIAHEC"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH00110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="383" mass="44473" checksum="CC7F24A70653A0AF" modified="2016-07-06" version="1">MGKGGRMPVADGEVQQAENSVRRVPHEKPPFTIADIKKAIPPHCFERSLIRSFSYLVYDLAVCFLLYYAATNYINLLPKPLSYLAWTAYVYVQGCFMFAVWVVAHECGHHGFSNYHWLNDTVGFVLHSLLLVPYFSWKISHRRHHANTNSLDRDENHIPRFKETIRSYYHHFNNPLGRVFIIAFTLTLGWPLYLIVNIAGRTYDRHASHFDPYSPIYSERERVQILLSDIGFLAGCYLVYRVALVKGFTWVMLVYGAPLHVVNGFLVMVTLLHHTHLSLPHYDSSEWDWLRGALATVDRDYGILNKVFHHIADTHVLHHLISSIPHYHAEEATEAIKPVLGDYYHYDGTPFYVAMWREAKECLYVEAEDNDKAKGVYWFKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YZM1</accession>
    <name>A0A164YZM1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YZM1"/>
    <dbReference type="Gramene" id="KZM95097">
        <property type="protein sequence ID" value="KZM95097"/>
        <property type="gene ID" value="DCAR_018339"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220386"/>
    <dbReference type="OMA" id="VECAFCT"/>
    <dbReference type="OrthoDB" id="730977at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040267">
        <property type="entry name" value="EID1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348">
        <property type="entry name" value="EID1-LIKE F-BOX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31348:SF4">
        <property type="entry name" value="PHYTOCHROME A-ASSOCIATED F-BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH01593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="297" mass="32417" checksum="072201166C2E9657" modified="2016-07-06" version="1">MVKTDVFSKLPDDVVLKIIFNLDDDPKDWAHLACVCTKFCSLLHTFCWKSKCTQTIPALVSDLLSGGAANSPPGGWAALYKLYFCCPGLAKAGVSVGNLEVGPSSLAQLGSFDNPISVFDSVVALADVVIGKGGEVSDVVMAGVVDDDSDGDDDGCNVCKRRKVCRLVGSHLACGARELSREQGNKLLASRFRGDCLYICGWPGCVHNDGKRNYMLFRGIFKNFKESRVWRTVSDGDRNKVNISCAFCSCTNTWNLHSAFCLRRAFGFHDDGELVVRAYVCENGHVSGAWTDWPLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z044</accession>
    <name>A0A164Z044_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0520995</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CONSTANS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM95115">
        <property type="protein sequence ID" value="KZM95115"/>
        <property type="gene ID" value="DCAR_018357"/>
    </dbReference>
    <dbReference type="OMA" id="GSAFHWH"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH01611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="379" mass="43220" checksum="619859C9B585BDE3" modified="2016-07-06" version="1">MTSCSQVRDGKIQENSSNKEKPSLCDFCGDSVAVLYCKADSAKLCLTCDREVHSTNQLFTKHTRSVLCDECESSPSSIFCSTHCFVLCQNCDWESHRISESSNHDRRPLEGFNGCPSVVEFLANFGFDDLGKKKSKRSEGVQESENFGFSDYLVWETPSVISLDDLISSDGSDHNFQVTGFPPLPKNRNTVCGQRKEEILCQLRELAKLEPGIDQNKQDVEPSPGFQSLLPYDDFQHQNLNFESKTEPTFFATYEGSTFQWCNDIGKDPEKILPNASSQSYFNTDYQVPAKDPDIDPKLHHIDNKNETPAQNIVVANASQIFPKVAPHEFCSQHRDIAITRYKEKRKSRRYEKHIRYESRKVRAETRARVKGRFAKIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZW67</accession>
    <name>A0A164ZW67_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_019748</name>
        <name evidence="7" type="ORF">DCAR_0522593</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH03197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZW67"/>
    <dbReference type="STRING" id="79200.A0A164ZW67"/>
    <dbReference type="Gramene" id="KZM96506">
        <property type="protein sequence ID" value="KZM96506"/>
        <property type="gene ID" value="DCAR_019748"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223238"/>
    <dbReference type="OMA" id="QDRRMQM"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF119">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A 59 KDA REGULATORY SUBUNIT B' ZETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM96506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="546" mass="61410" checksum="DF35C1D1878974E3" modified="2016-07-06" version="1">MIKQIFGKRKASSKSSSQSDSSNDVGYSSINTSSSAGGGVGNSTKTTSVSGKGANSASAALKSSNGNYAPQSSSAAIVSNQGKKSGPGGSQAGQGMNYGVYEALPSFRDVPSSEKQNLFLRKLDMCCVLFDFSDASKNLKEKEIKRQTLLELVEYISSATSKFNEVTMQEITKMVAANLFRAFPSSHDNKLPDVYDPEDDEPAMEPSWPHLQVVYEFLLRFVASTETDAKLAKRYIDHSFVLRLLDLFDSDDQREREYLKTILHRIYGKFMVHRPFIRKSINNIFYNFISETVRHNGIAELLEILGSIINGFALPLKEEHKLFLVRALIPLHKPKSVSMYHQQLSYCITQFVEKDIKLADTVIRGLLKYWPITNSSKEVMFLGELEEVLEATQAAEFQRCMVRLFCQIGRCLNSSHFQVAERTLFLWNNDHIRTLITQNRKIILPIIFPALENNTRSHWNQAVHSLTLNVRKIFIDADQALFDECLAKFQEEEMKGKEKLEKRELTWKRLEDMAASKAVTNEAVLVSKFSSVAIATSTNSPKAASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A302</accession>
    <name>A0A165A302_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_015810</name>
        <name evidence="4" type="ORF">DCAR_0414888</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A302"/>
    <dbReference type="STRING" id="79200.A0A165A302"/>
    <dbReference type="Gramene" id="KZM96828">
        <property type="protein sequence ID" value="KZM96828"/>
        <property type="gene ID" value="DCAR_015810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33430">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33430:SF1">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="2">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="2">
        <location>
            <begin position="128"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM96828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="474" mass="53386" checksum="E6BAD822A19001E5" modified="2016-07-06" version="1">MDNMPYQMISSLRPRTRTDWRLKVRVTRMWRQLTCSVETVGLNMIFVDELGGRIHAWLPAQQMHRLENQIIEGETKSAQVIFYKEMGESFNQAIHDVVQHLIIVIISSCKAQMLTGESYTKNMDNIPYHMINNLRPNTRNGWRLKVRLTRMWCQITRNAETVAIKLIFVDALGGRIHASIPAQYIHQLEKNIAEGETYDVNKFVVRRYADMQHGRCLKNDIYIQLNHMTEVMVTGGVDYIQQHVFQFTDLDALYDAAHEQKYLIDVVGILEQAGLVTHFRNRNAVFVGLSQASPGIRSLENRHECDAGPGVAKMLVLYEVVAFACFLLSSLVAKVLKLLFRLDGKQIRLFIRQGFGVKDALLLLTVFSSVTGIILLTLSVVNVVQIRIGLYSSGSAEARMAIWALCTIVAIALVIYVVSISVAIYASIIGDADIDPVLGQHHSSNRGNNNSADTDVAVQVRGLTQKKDDLVSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y7R7</accession>
    <name>A0A165Y7R7_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0417220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Y7R7"/>
    <dbReference type="Gramene" id="KZM98863">
        <property type="protein sequence ID" value="KZM98863"/>
        <property type="gene ID" value="DCAR_013775"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218194"/>
    <dbReference type="OMA" id="CEVPGHQ"/>
    <dbReference type="OrthoDB" id="692882at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042885">
        <property type="entry name" value="HIPP47/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932:SF12">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG97879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="144" mass="16025" checksum="2FDC911E616F519D" modified="2016-07-06" version="1">MQQRIVVRVPMKKEKDRSKAMKIVVGIIGVSSVKIGGEDKDIVIVIGNEVDAVTLTHSLVKKFGSATTLVRVEPFFNRGSGEDEYEAAAAAASVMYQGQQKYYGTYYGQPASYYYNPPPQYYPPPPQYYYSCDRPVDDSGCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZUJ4</accession>
    <name>A0A165ZUJ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_009201</name>
        <name evidence="13" type="ORF">DCAR_0310406</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZUJ4"/>
    <dbReference type="SMR" id="A0A165ZUJ4"/>
    <dbReference type="STRING" id="79200.A0A165ZUJ4"/>
    <dbReference type="Gramene" id="KZN00447">
        <property type="protein sequence ID" value="KZN00447"/>
        <property type="gene ID" value="DCAR_009201"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211032"/>
    <dbReference type="OMA" id="AGQRRQC"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000040">
        <property type="entry name" value="Nudix hydrolase 19, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015376">
        <property type="entry name" value="Znr_NADH_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001299">
        <property type="entry name" value="PRK00241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09297">
        <property type="entry name" value="Zn_ribbon_NUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="11">
        <location>
            <begin position="204"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN00447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG91158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="393" mass="43622" checksum="3E8BE5CF7F0953DA" modified="2016-07-06" version="1">MSIQLSSHVFAGNPIISKTPKLTDSVSFSSAFQTLKAYFSSDHGTQGSCPNFKVLPFRKGRPLVGSSGDLVPTWHLGWLSLSECQAFLGKSRVKLTENSFVYLGSELADEVVFWAIDVSDAGDLENEMSDMQLSFVDVMSLMVATDWTNQLVMADLAIAGQAKALLEWHITSCFCGKCGEKTVPIDAGRRKQCSNESCKKRIYPRVDPVVIMLVVDKQRDRALLSRQSKFVPRMWSTLAGFIEPGESLEEAVKRETWEETGIEVGEVIYHSSQPWPVGPSSMPCQLMVGFLAYAKSFEINVDKNELEDAQWFSREDVKKALTFAEYEKAQTTTAVKVKQMSKGVEKSFNLSLDFNMESGELASMFIPGPYAVAHHLIASWVNEAGVNDIQGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZZP1</accession>
    <name>A0A165ZZP1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0310562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZZP1"/>
    <dbReference type="Gramene" id="KZN00586">
        <property type="protein sequence ID" value="KZN00586"/>
        <property type="gene ID" value="DCAR_009340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009567">
        <property type="term" value="P:double fertilization forming a zygote and endosperm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080155">
        <property type="term" value="P:regulation of double fertilization forming a zygote and endosperm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000008">
        <property type="term" value="P:regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008502">
        <property type="entry name" value="Prolamin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31181">
        <property type="entry name" value="EGG CELL-SECRETED PROTEIN 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31181:SF67">
        <property type="entry name" value="PROLAMIN-LIKE PROTEIN (DUF1278)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05617">
        <property type="entry name" value="Prolamin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG91314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="14627" checksum="63370FA56368C74C" modified="2016-07-06" version="1">MASASHINLSLLFILAASMAMLAASSNVNTIQTQTSYNNKIDLATFEQCYSSVSECYDEILIFFRALARGLSLDTDYNIAPSCCSAVMDLHQKCWILLFPDPFYNFELRTFCARQTQGPAPAPAPVAAAPAMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A2C0</accession>
    <name>A0A166A2C0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Subtilisin-like protease SBT1.3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_009413</name>
        <name evidence="17" type="ORF">DCAR_0310644</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A2C0"/>
    <dbReference type="Gramene" id="KZN00659">
        <property type="protein sequence ID" value="KZN00659"/>
        <property type="gene ID" value="DCAR_009413"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215397"/>
    <dbReference type="OMA" id="HSNRTCK"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007870381" description="Subtilisin-like protease SBT1.3" evidence="11">
        <location>
            <begin position="22"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="14">
        <location>
            <begin position="30"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="142"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="381"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="15">
        <location>
            <begin position="670"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZN00659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG91395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="83216" checksum="BB1A3CE469AC4B3E" modified="2016-07-06" version="1">MVVKWVFLLLLASLTITNVVSLTASPFTKTYIVQIDRLAKPETFGDHVEWYASVIQSVSAELNDEENDDGENERIMYSYQTAFHGVAARLSLEEVERLQQHPAVMAVFPEVAYELHTTRSPWFLGLGSEESTSVWSEKLADHDVVVGVLDTGIWPESRSFNDTGLTAIPARWKGTCQIGRGFDKSHCNKKIVGARMFYHGYEAGAGKINEQEEYKSPRDQDGHGTHTAATVAGSPVQGANLLGYAKGTARGMAPGARIAAYKVCWASGCFSSDILAAVDQAVADGVNVLSISLGGGSSSYQHDSLSIATFGAMEKGVFVSCSAGNGGPTPVSLTNISPWITTVGASTMDRDFPSTVKLGSGQKITGSSLYKGRRKLSPAKQYPLVYTGGNSSILDPSSLCLQDTLNPKVVRGKIVICNRGVTPRVQKGQVVKDAGGIGMILANTEDNGEELVADCHLLPTVAVGEKAGRSIKEYAMTNSHPTATLSFQGTRLGVKPSPVVAAFSSRGPNYLSLEVLKPDIVAPGVNILAAWTNTLGPSSLATDKRIVNFNILSGTSMSCPHVSGIAALIKARHPDWSPAAIKSAIMTTAYIHDNNYTPLRDASTGAPSTPYDHGAGHINPLKALNPGLVYDLKPQDYFEFLCAQLSPGDMVVFAKFSNRSCRNTLASPGDLNYPALSVLFLEKGNNSVLTLHRTVTNVGKAVSNYHAVVSPFKSVVIKVEPTTLHFTKKHQKLSYKVTFIGKKLQAGPEFGHLMWKDGVHKVRTPVIVTRVPVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BRK7</accession>
    <name>A0A166BRK7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_011535</name>
        <name evidence="19" type="ORF">DCAR_0313040</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BRK7"/>
    <dbReference type="STRING" id="79200.A0A166BRK7"/>
    <dbReference type="Gramene" id="KZN02779">
        <property type="protein sequence ID" value="KZN02779"/>
        <property type="gene ID" value="DCAR_011535"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215270"/>
    <dbReference type="OMA" id="DPGKKTD"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000249">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="29"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="366"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="440"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KZN02779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG93753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="507" mass="57374" checksum="CF30640F46C25B65" modified="2016-07-06" version="1">MKKASEVSSSSKPTKQVLPHQTPRLTDHYLVGQKLGQGQFGTTYLCTDKSSGEDYACKSIPKRKLFCQEDYEDVWREIQMMHHLSEHAHVVRIRATYEDNLCVHLVMELCKGGELFDRIVQKGHYSEKKAAELMKTIVKVVEGCHALGVMHRDLKPENFLFDSPDEDAKLKAIDFGLSVFYKPGQYLSDVVGSPYYVAPEVLYKYYGPEIDVWSAGVILYILLSGVPPFWAETESGIFRKILKGKIDFESDPWPQISESAKDLIRKMLERSPRERITAHEVLCHPWILDDRVAPDKPLDSAVLTRLKQFSAMNKLKKMALRVIAESLSEEEIGGLKQLFRMIDTDNSGTITFEELKQGLRRVGSNLMESEIHHLMNAADIDKSGTIDYGEFLAATLHMNKMEREENLVRAFTYFDMDGSGYITFDELQQACKDFGVDEVKLDEMISEIDTDHDGRIDYSEFATMMRKGDGGVGSRSMRGNLNCNLAQALGTTHSTCLHGAVDPLKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CYA0</accession>
    <name>A0A166CYA0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_005327</name>
        <name evidence="10" type="ORF">DCAR_0205980</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CYA0"/>
    <dbReference type="STRING" id="79200.A0A166CYA0"/>
    <dbReference type="Gramene" id="KZN04490">
        <property type="protein sequence ID" value="KZN04490"/>
        <property type="gene ID" value="DCAR_005327"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206475"/>
    <dbReference type="OMA" id="WWCIRLV"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048354">
        <property type="term" value="P:mucilage biosynthetic process involved in seed coat development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009825">
        <property type="term" value="P:multidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000013">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033254">
        <property type="entry name" value="Plant_FLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382:SF0">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAS1" evidence="8">
        <location>
            <begin position="30"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="8">
        <location>
            <begin position="184"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN04490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG86762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="393" mass="42201" checksum="66A2FA20C2350E2C" modified="2016-07-06" version="1">MVITPRYSHSTPTLLLYFLLVSATTTPSLSLNLTAILSPFPDLSLFSSLLLSTAVYDDLSTVTSLSLLALPNSLLSPPPPNIADILRYHILLEYLSPSDLRHLPPSGRLIRTLFSTTTPISVNITRDTVNNSVNITPSDDGATSITVTSCVKTMPYNVSVYVVNAMLIPSSVDIMASESQPPLGLNITKALIDGHDFNVAAALLEASGVSDELESAENGAGITLFIPTDDSFADLLPSVKIQSLTADKKTVLLKYHVLHSYYPLGSLELIVNPVEPTFATDLASAGDYTIGISRLNGSVALKSGLTQASVTQTLFDEKPIVIFGINTVLLPKEIFWNITINTAVKVVLPETKDELESPTQLSALNESGALRRVGSGRYIAGMYCILGFLYVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D9G1</accession>
    <name>A0A166D9G1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0206527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D9G1"/>
    <dbReference type="Gramene" id="KZN04961">
        <property type="protein sequence ID" value="KZN04961"/>
        <property type="gene ID" value="DCAR_005798"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206070"/>
    <dbReference type="OMA" id="YHEKTDK"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010029">
        <property type="term" value="P:regulation of seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG87304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="382" mass="42517" checksum="7B6EA76100CAEB7A" modified="2016-07-06" version="1">MEDTAMEIEESSPIPRNSHRVALKNSIQTNFGDDYVFQIVNKDDWTSMAVSLSTNTVKLYSPSTGQFIGECNKHHSSTINQIMFSGDILHSCSSDGTVRAWDTRSFQEISCISAGPSEEVFCFSFGGVGDSLLAAGCKSQILFWDWRTRKQVACLQESHMDDVTQVHFVPENQNKLVSASVDGLMCIFDTSGQINDDDHLESVINVGTSIGKVGFCGKRNQKLWCLTHIETLSVWDWTDSRLEANFEDARSVASNSWTPEHVDYFVDCHYSYEDDSLWVIGGTNNGTLGYFPVKYNGGREILAPEAVLHGGHTGIIRSILPVSSIKGPASQNRGIFGWTGGEDGRLCCWLSDEACDTNRSWISTTLVMKTTKSLRKNRRQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DQI8</accession>
    <name>A0A166DQI8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_006381</name>
        <name evidence="13" type="ORF">DCAR_0207195</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DQI8"/>
    <dbReference type="STRING" id="79200.A0A166DQI8"/>
    <dbReference type="Gramene" id="KZN05544">
        <property type="protein sequence ID" value="KZN05544"/>
        <property type="gene ID" value="DCAR_006381"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209697"/>
    <dbReference type="OMA" id="PMGRHQP"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000435">
        <property type="entry name" value="SEC12-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF2">
        <property type="entry name" value="SEC12-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN05544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG87962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="381" mass="42275" checksum="55DCA3D324EDE18C" modified="2016-07-06" version="1">MEGKVGCASWIRRPENAHLVVLGTMKQLTHPASLQIFSFDPLTTSLSSSPKASYEFENGSEAVNIAVHPSGDDFVASTTSGDCMLFEYYGQKLLPKELHPLQGVGPQKCLAFSTDGSKFATGGVDGHLRIFEWPSMRIILNEPKAHNSFNDMDFSLDSEFLATTSTDGSARIWKTDDGVLITSLTRNSDEKFELCRFSKDGTKPFLFCTVQKGFKAVTSVWDISNWTKIGHKRLLRKPASIMSISLDGKYLAQGSKDGDVCVVDVKKMEICHWSKRLHSGSSIASLDFCPSERVVLTTSSEWGAMVTKLSVPADWKDWQIYLVLLGLFLASAVAFYIFFEKSDSFWNFPLGKDQTTRPKINTILGDPQSEDWGAFGPLDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EVJ7</accession>
    <name>A0A166EVJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ubiquitin-related modifier</fullName>
            <shortName evidence="9">SUMO</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208894</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with SAE2, SCE1, SIZ1 and MMS21. Interacts with HSFA2. Covalently attached to ABI5, FLD, GTE3, HSFA2 and ICE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EVJ7"/>
    <dbReference type="Gramene" id="KZN07031">
        <property type="protein sequence ID" value="KZN07031"/>
        <property type="gene ID" value="DCAR_007868"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206186"/>
    <dbReference type="OMA" id="ENDHINP"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000171">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="10932" checksum="46CC0E9F9C247342" modified="2016-07-06" version="1">MSGTTTADEDKKPGTEGGAHINLKVKGQDGNEVFFRIKRNTQLKKLMNAYCDRQSVEMNSIAFLFDGRRLQGAQTPDELEMEDGDEIDAMLHQTGGGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GD49</accession>
    <name>A0A166GD49_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0101429</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GD49"/>
    <dbReference type="Gramene" id="KZN08824">
        <property type="protein sequence ID" value="KZN08824"/>
        <property type="gene ID" value="DCAR_001480"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203783"/>
    <dbReference type="OMA" id="IYKNFDW"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000166">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG82266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="948" mass="105622" checksum="C4572AC541BC04E4" modified="2016-07-06" version="1">MEKSCSLLVYFDKGTPAIANEIKEALEGNDVEAKVDAMKKAIMLLLNGETLPQLFITIVRYVLPSEDHTVQKLLLLYLEIIEKRDARGMVLPEMILICQNLRNNLQHPNEYIRGVTLRFLCRLNEVEIIEPLIPSVLANLEHRHQYIRRNAILAVMAIYKLPHGEQLLVDAPEMIEKVLATEQDQSAKRNAFLMLFTCAQERAINYLLTHVDKVSEWGELLQMVVLELIRKVCRTNKAEKGRYIKIIISLLTVPSSAVVYECAGTLVSLSSAPTAITAAANTYCQLLSSQSDNNVKLIVLDRLTELKSAHKEIMVNLIMDVLRAVSSPNLDIRRKTLDIVLELITPRNINEVVLTLKKEVMKTQSGELEKNGEYRQMLIQAIHSCAIKFPEVASTVVHLLMDFLGDSNVASAMDVVVFVREIIESNPKLRVSIITRLLDTFYQIRAARVCSCALWIIGEYCLSLSEVESGITTIKQCLGDLPFYTDAEEGESADSSKKPQQVDSITVSSKRPAILADGTYATQSAASETAFSPPTVVQGSLSSGNLRSLLLSGDFFLGAVVSCTLTKLILRYEEVQSSKVEVNKASTQALLIIVSIIQLGQSSFLSHPIDNDSHDRIVLCIRLLCNTGDQVRKIWLQSCRESFVKMLSDKQLRETAEIKAKAQISFAQPDDLIDFYHLKSRKGMSQLELEDEVQDDLKRATGEFIQDGDSANKLNRILQLTGFSDPVYAEACVTVHHYDIVLDVTVINRTKETLQNLCLELATMGDLKLVDRPQNYTLAPESSKQIKANIKVSSTETGVIFGNIVYETSKVFERTVVVLNDIHIDIMDYISPAVCGDAAFRTMWAEFEWENKVAVNTVIQDEKEFLDHIIKSTNMKCLTGPSALEGECGFLAANLYAKSVFGEDALVNISIEKQADAKLSGYIRIRSKTQGIALSLGDKITLKQKGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GKS8</accession>
    <name>A0A166GKS8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cellulose synthase-like protein D1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_001721</name>
        <name evidence="14" type="ORF">DCAR_0101698</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GKS8"/>
    <dbReference type="STRING" id="79200.A0A166GKS8"/>
    <dbReference type="Gramene" id="KZN09065">
        <property type="protein sequence ID" value="KZN09065"/>
        <property type="gene ID" value="DCAR_001721"/>
    </dbReference>
    <dbReference type="OMA" id="IMSKVPD"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000089">
        <property type="entry name" value="Cellulose synthase-like protein D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="818"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="946"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="976"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1009"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZN09065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG82533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1044" mass="117214" checksum="0A1CC2C50892CB9A" modified="2016-07-06" version="1">MAGSNNHRKTSSTSGANRSSDGVKFQRRTSSGRLVSLSRDEESENSGEYAGPNDYINYTVMMPPTPDNQPGASSNKADGQGSSQSGTQDSQRPGKTKRAGGGDDAGGESGKMDRRMSVMKNSNNNKSMLLRSQTTDFDHNRWLFESKGKYGIGSAYWPPEDDDYANENGVSPADFLDKPWKPLTRKINVPAKILSPYRVLICIRLVVLVFFMTWRIKNPNKDAMWLWAISIICEMWFAFSWLLDVLPKFNPINRATDLNALKEKFETPSAANPNGRSDLPGVDIFISTADPEKEPPLVTANTILSILACEYPVEKMSIYISDDGGAILTFEAMAEAVNFAEVWVPFCRKHNIEPRNPDSYFSQKTDPTKNKKRPDFVKDRRWIKREYDEFKVRINGLPEVIRKRCDMHNSREGKKEKQLAKDNNGGVMPPDQDIKVTKATWMADGTHWPGTWYSAVADHKKGDHAGILQIMSKVPEPDPVMGQLDEKKLDFTDIDIRIPMFAYVSREKRPGYDHQKKAGAMNALVRASAILSNGPFILNLDCDHYLYNCMAIREGICFMMDRGGDRICFIQFPQRFEGIDPSDRYANHNTVFFDGNMRALDGLQGPVYVGTGCMFRRYALYGFHPPRANEYSGLVGQNKAKAANVPMNADDAETQPLTEDHPDLGVPKGFGNSTMFFESIAVAEYQGRPLADHVSIKNGRPPGALLTPRPPLDAPTVAEAIAVISCWYEDKTEWGDRVGWIYGSVTEDVVTGYRMHNRGWRSVYCVTKRDAFRGGAPINLTDRLHQVLRWATGSVEIFFSKNNAFLASRRLKFLQRVAYLNVGIYPFTSIFLVAYCFLPAFCHFTGEFIIKQLDTMYLLYLLTITVTLALISTLEVKWSGISLEEFWRNEQFWAIGGSSAHLAAVIQGLLKVIAGIEISFTLTTKATAEDDEDIYAELYIVKWTSLFIVPLTIIIVNLAALVIGCARTMYSEIPEWNKLMGGCFFTFWVLAHMYPFMKGLMGRKGRLPTIIYVWTGLVSITVSLLYITIAPHDGLRAGSDGNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I592</accession>
    <name>A0A166I592_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
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    </dbReference>
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        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A166IQ40</accession>
    <name>A0A166IQ40_DAUCS</name>
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        </submittedName>
    </protein>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="1921758at2759"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="81" mass="8601" checksum="CC1D2839CEDADE2B" modified="2016-07-06" version="1">MGGSGDDDGCQCRPLGFLLGLPFAFVALILSLIGVVIWIVGLALTCICPCCLCVTVLVELAISLIKAPFSIIKWFTEKIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IQK8</accession>
    <name>A0A166IQK8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_004037</name>
        <name evidence="5" type="ORF">DCAR_0104305</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KZN11381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IQK8"/>
    <dbReference type="STRING" id="79200.A0A166IQK8"/>
    <dbReference type="Gramene" id="KZN11381">
        <property type="protein sequence ID" value="KZN11381"/>
        <property type="gene ID" value="DCAR_004037"/>
    </dbReference>
    <dbReference type="OMA" id="KKGDKFQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF100">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN11381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG85118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="41528" checksum="93BE9693349A2A3D" modified="2016-07-06" version="1">MAGTVFLRTNRFEVKLEAITFKTKEFDQLCTMDFLFPRGKVWQHTPVVGLDVIRHPRDPNIVLLLLCFGVGCVILRFRAGDCLSEPILKFLTDKRITFVGFGIPEKNHLLPFDELGLDAWKTDVGYMAAKLRNNAKYAKCDLAELARKVLGIKEMMALTDASSFERHERIKSAMCQLFLSSAIAMTLLGADSKNILRGSPKKFSCLKRFNTMKLVNEAWSKLVKGKKDIGSKFSEVVHSYYGVRTDAYPDNVAHDCFVSKVWKGYGRGNFFQTKIPEDESGEDSVRVREGGRLGDDYARENEGVSGDGTPGKENSSPSTKPLKGILKCPHKNKNHCRYSIPSSPTSLSPSPTLKRANSKGHNVTFKCDMGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J546</accession>
    <name>A0A166J546_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_004455</name>
        <name evidence="9" type="ORF">DCAR_0104819</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG85628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J546"/>
    <dbReference type="Gramene" id="KZN11799">
        <property type="protein sequence ID" value="KZN11799"/>
        <property type="gene ID" value="DCAR_004455"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221904"/>
    <dbReference type="OMA" id="WECNAFE"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000009">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF147">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN11799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG85628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="365" mass="40616" checksum="58B725FE16FD412D" modified="2016-07-06" version="1">MMLGESEPLHRPNPTVQIPLYDDPTAEIHSPFSVTPNDHHSPHCTLTALHRYLPSVEDIDIPLDAFTCDHFRMYEFKVRRCARARSHDWTECPYAHPGEKARRRDPRKFHYSGNACPDFRKGACVRGDACEHAHGVFECWLHPARYRTQPCKDGTMCRRKVCFFAHTASQLRVLPPSSPSQLGIDSLSPGRCGFDSFFTGSELTPSLPSRLGCDSFVRKASFGSSPTSILYSPSESPPESPCGLLGFDSVSELSESMRGMQIGHGSMSMSVSPPWGFQMGSSYGSPRQCSMIRPGFMSLPGTPTRKMTRSGLGGFDLWDQSCEEEPAMERVESGKDLRAKMYAKLSKENSLVRVDSDNSGSELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J7E7</accession>
    <name>A0A166J7E7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0104900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_004528"/>
    </dbReference>
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    <dbReference type="OMA" id="RQIFCDE"/>
    <dbReference type="OrthoDB" id="10251073at2759"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOG85709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="14925" checksum="8E4E617891FE754A" modified="2016-07-06" version="1">MASLSRVFTRYYSSVMAAAAKSAVADATAAVKKTAVKKTAAKKSAEKVATGPKKLTGILKPMPISPALTEFMGAAESSRTEAVKRVWEYIKANNLQNPQNKRQIFCDEKLKTIFDGKAEVEFIEVAKLLSQHFVKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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    <name>A0A169WF03_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0314228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WF03"/>
    <dbReference type="Gramene" id="KZN03820">
        <property type="protein sequence ID" value="KZN03820"/>
        <property type="gene ID" value="DCAR_012576"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG94931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="6333" checksum="F19BE30B3020925A" modified="2016-07-06" version="1">MPLILNWVDLGNTNLSGVLVLDLGKLQYIQYLRLSNNRLVGRIPRELVNVSTLKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFY9</accession>
    <name>A0A175YFY9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_030155</name>
        <name evidence="13" type="ORF">DCAR_0935030</name>
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    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CONSTANS family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH15489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_030155"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200187"/>
    <dbReference type="OMA" id="VTESYAM"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCT_domain"/>
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        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
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        <property type="entry name" value="CONSTANS-like_ZF"/>
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    </dbReference>
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        <property type="entry name" value="CCT MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="14"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
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            <end position="391"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44771" checksum="A322AC03E5148B09" modified="2016-09-07" version="1">MISSKKLASAVGGKTARACDGCIKKRARWYCAADDAFLCQACDSSVHSANLLAGRHERVRLKTASTERGSVEPSWHNGFTRKPRTPRKSKLQGVQKNPIHLVPEMGQDENSFEENDEQEQLLYQVPNLDLLSDQLCLAKQGKKSDGTINYDKYSLRGLSESNNDIEEFAADVESLLGKGLDEESFDMEKLGFLNCREKDLVESCDRSIETVKLEENKDTAVTMGINIGEIDITEKFELNFDDYESTENCAEDNEAGANGGTCVENEEFVVRNGDELIGKKRNRLLALDYEGVISAWADQKRPWVIGEGRPELDLNDFWPADFMEVCGGVDNQAYGEMGGSSNLMADGGREARVLRYREKRRRRLFCKKIRYEVRKLNAEKRPRIKGRFVKRPIDNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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    <name>A0A175YI15_DAUCS</name>
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        <recommendedName>
            <fullName evidence="12">Protein root UVB sensitive 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_030041</name>
        <name evidence="10" type="ORF">DCAR_0934902</name>
    </gene>
    <organism evidence="9">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the RUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_030041"/>
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    <dbReference type="OrthoDB" id="364779at2759"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF24160">
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        <property type="entry name" value="UVB_sens_prot"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
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            <end position="284"/>
        </location>
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    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="8">
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            <end position="431"/>
        </location>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04884"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF24160"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM82472.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH15364.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="49471" checksum="9460036AACE04EB7" modified="2016-09-07" version="1">MSMGNGEAPSEITLEEWSGTASSKFFKTATITANSSTHFAVQRHGSRFHHISNRFLEAFVPEGFPNSVTPDYVPFQVWDLLQGLSTYVRMMLSTQALLSAIGVGEKSATVIGATFQWFLRDLSGMLGGILFTFYQGSNLDSNAKMWRLVADLMNDLGMLMDLVSPLFPSAFIFIVCIGSLSRSFTGVASGATRAALTQHFALQNNSADISAKEGSQETVATMAGMAIGMLIAHITMGQPIAIWFSFLSLTMFHMYANYKAVRCLSLTTLNCERSSILLWHYVETGQILSPKQVSVMEHVLPLWITSWTSRKFTYLYTRVHLGVRISSVDHLELKELIQLAGSHYKKAKYLLHEKRGVISIIMHKDSTAGDVLQAYIHALVMAHLVEKGSSVYLESQSWMDKHYKVFSLKLRSSGWKLERLLTPSIIWRAHWRRDCLDEKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YIG5</accession>
    <name>A0A175YIG5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_031063</name>
        <name evidence="6" type="ORF">DCAR_0936042</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4A6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF93">
        <property type="entry name" value="PECTIN ACETYLESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041768637" description="Pectin acetylesterase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG81355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="462" mass="51698" checksum="0A2C86B01835E6B8" modified="2024-05-29" version="1">MATARLAPWLLLLVCVLSVLRTESVDVVNTIVTDAVKKGAVCLDGTPPAYALHKGVGDAANNWLIYLEGGGWCATEAECVFRTTKDVGSTKRWAVEKSSLIAMLSDQESENPYFYNWTRVYVRYCDGSSYVGDREKPYVVRYHDVDPGNNTTVYFRGARVFKAVMEDLLEKGLKHASNALLTGCSAGGLGALLQCDNFRSILPEKAKVKCAADAGFFIDGTDIYGGHMHRERFAQIVQTHESQKNLPKSCTSKMDASLCMLPQNFVADIKTPLFVVNSVYDTWQIENILMPVKKDQLAVHDEFASCGHDIENCTCDQVKLWRGFQSEFYQALAQLNSSSSNGMFINACASHCQTQKQETWFGTSTMPKLRDRPGVGEAVGEWFVNDAEMRRVDYRTKTPLVCEYNPEEPHWNNHYTFRGTNLFNPAYTLADEIKRKTSLIPRCPAEIPAYFQQPLLCIAGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4X7</accession>
    <name>A0AAF0W4X7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0101343</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the lipoxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4X7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="13">
        <location>
            <begin position="122"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG82181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="808" mass="92207" checksum="3C70E32E1F9DDF81" modified="2024-05-29" version="1">MLKPQSVLNQHYSSCFSNKDQLPWHNAPFSSRSLPLVTNQLNRKDYYPSNKSECYVCAAVAKPSNCNDPALNNIQLTATIIVEAGATGVLSYLSKLGRVKSGRVGVELVAANKCSEDGEKRKSIQQDAEEGKGGYTCTFNVEHRFGDVGAIFVKEIYFDGYPGGRIIFSCESWLQPDSNRIFFTNKSEDLKRKVLGGNPTYPYPRRCRTGSPSSKKEIFSNPRIKDKIYVPRDENFSEMKLSSFNSAKRHAFIPAIENILLKEDERRFSSFAEIDQLFDENDNLSTECKETPSKILPDLGSFLRFRTPELLNKDKFAWMKDEEFGRQTLAGVNPCTIQLVREWPLKSNLAQEYYGEPESAITKELLESMMAECMTVEKAIEEKRLFVIDYHDLMLPFVEKVRKTGGRNLYGSRALFFRTTSDTLKPMAIELWKEAYTPSTHGTAGWLWMLAKAQFLALDSCYHQLISHWLRTHCVVEPYVIATNRQLSSMHPIYRLLKPHFKDTMAINALARESLVNAGGIIESAFSPGKYSLEMSSVAYRDLWRFDQEGLPADLIRRGMAEEDPTSDTGVKLTIEDYPYASDGIVMWTIIGQWVNAYVNHYYPDETDIEFDYEIKNWWTEIRTVGHRDKKDETWWPKLDTSEDLTGILTTMIWIASGHHAAVNFGQYDYAAYFPSRPTIARAKMPTEDQTDESWEDFISRPEDMILSCFPTCDQACMVVATLNVLSKHSEDEQYIGDKPEASWAENAVIKAEFELFRERLMDLNKIIDERNKKLKNRSGAGVIPYTLLKTTCQDKLKGMGVPNSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5S4</accession>
    <name>A0AAF0W5S4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0101380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5S4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF39">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="5">
        <location>
            <begin position="14"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="175" mass="20572" checksum="FE26335C6EA0DD7F" modified="2024-05-29" version="1">MLKLKSVDSSSQNYSYDVFLSFRGEDTRKNFTDHLYKALQDAGLFTFRDDEDIERGESIKSELENGIQQSRSWIIVFSENYAFSSWCLDELVMILKCRNRSKRLLLPIFYHVDPSDIHKQSGCVYEAFSRHEEKFKREVNDTKRKDLMEKIQQWRTALTELANLGGMHLQNLTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5T1</accession>
    <name>A0AAF0W5T1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="11">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0102958</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:11577"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="11"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5T1"/>
    <dbReference type="KEGG" id="dcr:108210882"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.320:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10240:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="12">
        <location>
            <begin position="184"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG83780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="259" mass="28555" checksum="78A67F2904516DE5" modified="2024-05-29" version="1">MDVMIHSSSFLLPELPADQTSGAGPTYALVLLNQHLPRFTPLLWKHAQLRVCADGGANRLYDELPGLFPDEDASSVRNRYKPDMIKGDMDSIRAEVLEFYKNMGTKIVDASHDQDTTDLHKCVAFVRDFYADTETSTLCILVVGALGGRFDHEMGNINVLCRFSTIRIVLVSEDCLIQLLPSTHHHEIHIQSSIEGPHCGLIPIGTRSGSTTTTGLQWNLTNTEMRFGGLISTSNIVRGGIVTVQSSSDLLWTVSLKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W912</accession>
    <name>A0AAF0W912_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W912"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="F-box_FBXO41"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box/LRR-repeat protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000648">
        <property type="entry name" value="F-box/LRR-repeat protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
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        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF57">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="160"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="949" mass="104027" checksum="9DA8A4E158AEA6DA" modified="2024-05-29" version="1">MGTGEEDKNVEELEFEKLAECLAAGSGSMDEILDGVNLKSFHNMDGSSSSVPSVMGVDLSSALRGEPSSSAVASERENLDHDTHNKRPKVHSFSLDWDCQLASASSDFLERGYNINQGSFKNEFHYLSPILAEGEPENLIVSSSGRDKRDGCNTPQVVDMELRMDLTDDLLHMVFSFLNHINLCRAAKVCRQWRTASAHEDFWRILNFENRSITLLQFEDMCRRYPKATQLNISGAPAIHLLAMTAISSLRNLEVLILGKGQVGETFFQELTGCHKLRNLIVNDATLGNGIQEIPIYHDQLQNLQIVKCRVLRISVRCPQLQTLSLKRSSMAHVALNCPLLHDLDIASCHKLSDAAIRSAAISCHLLESLDMSNCSVVSDETLREIALACANLHVLNASYCQNISLESVRLPMLTVLKLHSCEGITSASMAAISYSYMLEVLELDNCSLLTSVLLDLPRLQNISLVHCRKFIELNLRSVVLSSIKVSNCPSLQRINLTSNSLQKLVLQKQESLTTLALQCQSLREVDLTDCESLTNSICEVFSDGGGCPMLSSLTLDNCESLTVVSFQSTTLVNLSLAGCRALSSLKLNCPYLENVSLDGCDHLELASLCPVGLNSLNLGICPKLNSLHIEAEHMVLLELKGCGVLSEASINCPLLTSLDASFCSQLRDDCLSATTTSCPLIESLILMSCPSVGPEGLSSLRWLPHLTSLDLSYTFLMDLQPVFDSCVQLKVLKLQACKYLTDSSLEPLYKNGALPSLCELDLSYGTLCQSAIEKLLACCTQLTHVSLNGCVNMHDLDWGSELGQLAIDIELSAEQPNRLLQNLNCVGCANIKKVIIPSAARCLHLSSLNLSLSANLKEVDLACLNLCFLNLSNCCSLEILKLDCPKLTSLFLQSCNINEDAVEVAIEQCHMLETLDVRFCPKIHPVSMGRLRAACPSLKRLFSSLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBC9</accession>
    <name>A0AAF0WBC9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Agglutinin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0104867</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aerolysin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBC9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20216">
        <property type="entry name" value="PFM_HFR-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.15.10">
        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055267">
        <property type="entry name" value="Aerolysin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008998">
        <property type="entry name" value="Agglutinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036242">
        <property type="entry name" value="Agglutinin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053237">
        <property type="entry name" value="Natterin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244:SF5">
        <property type="entry name" value="NATTERIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244">
        <property type="entry name" value="NATTERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01117">
        <property type="entry name" value="Aerolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07468">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00791">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50382">
        <property type="entry name" value="Agglutinin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agglutinin" evidence="3">
        <location>
            <begin position="6"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin" evidence="3">
        <location>
            <begin position="161"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="506" mass="56900" checksum="A6DE4388A408194B" modified="2024-05-29" version="1">MSQQVVALPRFVEFRSQNSNKLLRYVKEDGAVKGFVQFSGDDVGSADAKFEVHRSTTVNGLVHIRSCYNNKFLQVLDNTHNWLAAKADKPDEDQSSRSCTLLRPTSVNGNPKTIRLTHVNLKLNLCFWRGDPYYACVIAYYSDPDAGSCDVFNVIDWQSLVILPKYVTFKGANDKFLSTLLFHSNTYLSFSTTDLDRDPLSAGNEIVQTSNGNISIRNLKDKYWMSSTEWIFADSTDPTSRNSIFQPVKCSDGTIALRNLANNYFSTMMTSGNIRDCLNVGSTAITRFAKLKVSEFVMSKTIENINFRLMDARIYDENVLVMVSSTYANQGNSTQEQNFNLSYRNRRSSTWNCNFSVKESFQASFSAGIPVITDGKITFGFDVSEQYTWGETKETEETISTSYKVNIAPKTKVTMRIVATRGICDVPFYYDQRDVLYTGETVVTQMDDGVYTGVNNYSIYAEEKEEPLSQSTRIAAAAGFKRVAKVSPTGVITRLKHPIISYGQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WD81</accession>
    <name>A0AAF0WD81_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0206469</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WD81"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000299">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057597">
        <property type="entry name" value="ALE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF25">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23180">
        <property type="entry name" value="ALE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="389"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG87246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="725" mass="79387" checksum="B1578D5815E5FCB8" modified="2024-05-29" version="1">MSGLFLMFGASGELKKFRRLLLKWMIISLYAIFSTCADLPNTPVEEQVISPTNRPLLVSAIPKIPMLVDLPVFYKRHRKHIIHNPVPNLPPAPAHSPYNSPLIASAHAPSNSHFSKPSMRKSKLLPPTASMTPPWLHNVSPTQSDPGAIPAVLANPPKAPNCCGHNMVRKRGTQACHCVYPIKLDILLINVSSNPNWNIVLEEFAYQLGLRGSQIAPINFYVVTSSNWNISVAITPHTGKSFSAVEASGINSSLSMHRVRTNPKLVGDYKILNFTWFQSQASSQASPVGAPSYNPSSSTELNTSSNGKHPSIVLIVGVGIGVLIIGIISMLIVCSYTSQQGKKKEKTYVTETAKSKTVDALPRVGSLSLHPTSTRFLAYEELKEATNNFDSISVLGEGGFGRVFKGMLSDGTAVAIKRLTSGGQQGDKEFLVEVDMLSRLHHRNLVKLVGYYSNSDSSQKLLCYELVPNGSLEEWLHGPLGLNCPLNWETRMKIALDAARGLAYLHEDSQPCVIHRDFKASNILLENNFQAKVADFGLAKLAPEGRQNYLSTSVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGQENLVTWARPILRDKDRLDELADLRLGGKYPKDDFARVCTIAAACVAPEANQRPTMGEVVQSLKMVQQVTEYQDSVLTSNNRPTFTSDGTSSIFSSGPYSGISAFENDVISRTTVFSEDLHEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WE20</accession>
    <name>A0AAF0WE20_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0207054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108206966"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:RSF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rsf1-like"/>
    </dbReference>
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        <property type="entry name" value="DDT DOMAIN-CONTAINING PROTEIN DDR4 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
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    </dbReference>
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        <location>
            <begin position="309"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
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        </location>
    </feature>
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        <location>
            <begin position="321"/>
            <end position="361"/>
        </location>
    </feature>
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    </feature>
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        </location>
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        </location>
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        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG87822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="60364" checksum="8731123C6DA5E4F4" modified="2024-05-29" version="1">MDVTDDRKLAPSSVIDHRTKLRRRCELATVLNFLTVFEPLLKSKMKVTAEDIEMAIIEPNELLSKLHVMLLKGIPPVSKMLDRTDAWVTVLCKKLSDWWPWVANGDLPLTVNKGKEISEYKELEPTTRLVVLKALCEIRLQQDDAVSYINNAMKTGSEVSTFQKHRICGDEKGTSYWVDGDASIGYRLYKEVRKFESKQNDEGDGYFPAIDIQWETLATSLEEFRKVVVDFSSSKIKLEVDVCKTVEANVLPVLEKLHKKRERAVKQQKSREMILSGSQFSGLTRTCRTRTAVCYTFDEYDKRISEAISVQQQQTKKRKTTKEPVAVKKHSDNEKETETASEETMDRIEESEHCNSKKETENTSQEILQSDMKVQNDIVADSETESENHQDVDIDDNMQSQDIVSDSETESESHHDGDIDDNSDVEWDEDINDASSEDGIASNSSDKENKNLDKNHVTCARRRQGLPQRKRVAGVVSPAQENSNKGAKARPRGRLTRKDSLDSFVAPDSEGESSPADTSGRSSSGNLSDDSVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WF78</accession>
    <name>A0AAF0WF78_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0207262</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG88029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="324" mass="37156" checksum="83D5271AF4820B3D" modified="2024-05-29" version="1">MGRAKAKMELIKKDKVLNNTFRDRSATLKRKVEELATLCGVKVVVIIYGPKQGTSSPIVPEVWPQDRDQVVELIDKYRGQSAEDRANRTTLVSDLFKERNKKAEKDLKEARKKRIQAKYPTALDPVFDGFREDEIRNSVVFLQSKIDNAKAKLEQMKANNSIRRACHVQQQGVMLSKKRSLDLDAGNQANKYMKRHPHEAIAEPMRVPVAPMPLQIQHQRFPVPDYDQNRVMLRFVDGYVGGASNMMHNAPLRQVVYGYPRMEGTLDSINYANNLAMVPQNYYGEGIQPIAQQVLEYHPMPQESSSHQMAAASHQYYQDGKWQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WH23</accession>
    <name>A0AAF0WH23_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">FYVE finger-containing phosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">PIKfyve</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0208650</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
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        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
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        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="21">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
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            <begin position="1469"/>
            <end position="1785"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="1796"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
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            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
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        </location>
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        </location>
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        <location>
            <begin position="710"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="20">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
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            <begin position="1799"/>
            <end position="1818"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00012009"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
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            <dbReference type="EMBL" id="WOG89412.1"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJY7</accession>
    <name>A0AAF0WJY7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0309985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJY7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG90741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11629" checksum="AF0CDE7BBDBEA728" modified="2024-05-29" version="1">MSAVQSWFNKLEDVAHVADAFMDELAYEVTRRKVENRGTLRHLFSTKNSVLYRSKVAHKIKSIHTSLDNGDISLSDNTRKIQEGKWGGMVQNPTHQTNRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN86</accession>
    <name>A0AAF0WN86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0312348</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN86"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF142">
        <property type="entry name" value="FLORAL HOMEOTIC PROTEIN APETALA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="159"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="251"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG93067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="512" mass="57481" checksum="959DA4E2EB370470" modified="2024-05-29" version="1">MWNLNDSPSHERDEEESEEGCSSPFDGDGPEFDKGKRVGSMSNSSSSAVVIEDGSEEEDGSRRKRSSSKIFGFSVNNDNSPELEPPVITRQFFPVDESVETMGIASEASATSTFPRSHWVGVKFGQTEPPRTAGVAGKSTEVPQPLKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGVEADINFVLEDYEEDLKQMSNLTKEEFVHVLRRQSTGFPRGSSKYRGVTLHKCGRWEARMGQFLGKKYVYLGLFDTEIEAARAYDKAAIKCNGKEAVTNFDPSIYEIELHPTETSSNQRDHNLDLSLGSTGSKQKELGDDHSHNQQGRDQQSASMGYPMNSWQNRGIRPMQESGRNQLESQRRDGYNEMETLQLLSQTHLQSPGSARPQDMHSAYWNNYTKVSEVQQGVHMFPTQHNSNYQMQFSSSSSIGGQSSASDYHNQWRSSGPQVFASAAASSGFPPQQIVRSDPNWLHTNGFHSYMRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRJ3</accession>
    <name>A0AAF0WRJ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0314104</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108214308"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="106"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG94807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="19082" checksum="4433366B2A11A760" modified="2024-05-29" version="1">MQVHSSSPLVPLMSDHTTPVFGLSPIVVAIIAIICAAGVLLAYHCVTARWWNEVEPDERQQLPQLPVTYAGEGQSSFEDSIAELIPSYKYTPEISSVSKTQDGGTCSVCLSEFMEGELVRVLPECLHAFHVSCIDMWLQSHASCPLCRAGTPTPPRRHALSGGSTPLRLSSPMYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WS05</accession>
    <name>A0AAF0WS05_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0312319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-containing protein HOS15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ebi-like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
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        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
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        </location>
    </feature>
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            <begin position="379"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG93040.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="60975" checksum="0A135DD7B6F407E7" modified="2024-05-29" version="1">MVPPITPLELNLLIFRYFQDSGFTHSAFTLGCEAGLSDCPIDGNMVPPGALVKCITKGLQYLEMEANLSKRKADLDEDFSFIRPLDLITKNVNELRQMITERRKNRQKHVVKVINRKDGSRSRGVSEKGKLANEKEHEKYKHLGQSSGNAFVKDTEMVSAEEDKQLEIFGGSRPMHIATTPTPKHYAYGVLGVDVTILEGHASEVCACAWSPDGSLLASGSVDSTARIWKMAERINGGKSGDGNPNVFVLEHAQGRRNKNKIVTSVDWNGDGSLLATASYDGDARIWTLDGELKSTLRKHKNAVISIKWNKTSDYILTGSLDKTAIVWDVETNEVKQQFELHSGHVLDIDWRNNVSFAASTNKLIHVCKIGMNYPVKTFYGHQSEVNSIKWDPTGSLLASSSDDYTLKIWSLKQDNYIHDFTEHRKGVSTSRWSPTGQGTDNPNKQVLLASASFDSTVKLWDVEQGKLLSNFTGHRDAVYSIAFSPDGEYLASGCKDKSIHIWSVKTGKIVKSFKADGSIFEVCWNKEGDKVAACTNRKTVLVMDFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT62</accession>
    <name>A0AAF0WT62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4G</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein synthesis initiation factor 4G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0414910</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG95585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 4G"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="1584"/>
            <end position="1708"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00075135"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51366"/>
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            <dbReference type="EMBL" id="WOG95585.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
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        </source>
    </evidence>
    <sequence length="1772" mass="193738" checksum="7958A8CE1FB820EF" modified="2024-05-29" version="1">MSVNQSRAEKSESVRYQKLNRSGSSGVQRNYSGGGGKGSGAATAPTPSLSTNRSFKKPSNAQGSQTRVVNSDIRSDATTGGNVAVSGSGRGVQNGAHPQQHGGSDASVVVKQNVMPSTQKVNRGVPKAPAPNSTPVSSNTVVPSTPAKVSCGSGGGFPLQFGSISPGLMQVPARTSSAPPNLDEQKREQARYESLRAAPVLPAQSVPKQNVPTNNSGSADQTNVVDTHSMSKARRDVQIASGPHTIQTQKPSLHPISGMPMQMPFHQQHIPVFGNHPNPQLQSQSMVNSSMPMPMAMPFPMGNPSQVQQQLYIQGLPPHMLPPQGVMHQGQGVNFSSQMGTQLPHMGNMGMNINPQFPQQQPGNFGGARKTVKITHPDTHEELSLSKKADTYVESGSSAPRSHTSIPPQSQPISSFPPGRPSNYYPNSYSQGSVLYTGPNSLHLNSNQVAPSSQAPRVYKQVTVKPAAPRVEKIAESSLPASLPTVEKNIPKISGREGEATSIRSERDSENITDMSLPKSESTSMPAQSKAAIGVSDTVSASSPPSSSSTLPEISEHLKSTSSRTTVIEKTDLLNRSNSNDNDEKHGKKSQSLLQNQIGGSSVSVSPSSLEVIDNTIESALPEAGPGEGYVGESKTTCVSVDLNSNIAEVHGIHELAKSDGVDSAEMSTATSVHYNSKQEAMGAQESRHILLSDEPTADATGCASKPNIPKPSDHADQTESLVVEVKETQPKLEKEPVHLTKGEEDADSSTSIPGAVDRLITETSILSLGSTISNETEKTSASDASTKKDDTVGTIEVSTVEPDKLDQKSQMPSIPYHLLSKDKPVPETNMSKNTNAKKKRKEALQKADRAGTTADLYMAYKGPDEKKENLIDNESSETSSSISKELAASISQKDVPGESKAEPDDWEDAADLSTPKLDDGKHLGEVKDHIEDKSLMDRKYSRDFLLKFSEQCKDLPEGFEITSDIAEALVLSIGNVPREMLPSPGRNDERSMGRSRSGRRGSGMGDDEKWNKVLPLPSGLDMGYGNHGNNVAFQPGGNFGVLRNPRVQSPVMYSGGILSGPVHSMGPQYGMQRTNSDADKWQRATNFQRGLIPSPRTPAQVMHKAERKYEVGKITDEEQAKQRQLKAILNKLTPQNFERLFEQVKQVNIDNAGTLTGVISQIFDKALMEPTFCEMYADFCFHLAGDLPDFNEDNEKITFKRLLLNKCQEEFERGEREQEEANRAEEEGEVKQSDEEREEKRVQARRRMLGNIRLIGELYKKKMLTERIMHECIKKLLGQYQNPDEEDVEALCKLMSTIGEMIDHPKAKVHMDAYFDMMAKLSNNMKLSSRVRFMLKDSIDLRKNKWQQRRKVEGPKKIEEVHRDAANERQAQANRLARGPSMGSSFRRGQQPMDFAPRGSNVLSSPNAHMGGFRGVPQQPRGYANQDIRTDERHSFDNRNLSVPLPQRPLGDSITLGPQGGLARGMSIRGQPPMLSIPFSDMHNQDSRRPTTGLNGYGSVTDQRPVFTSREEIISRSASHRFVSPAAHDHMNLMDGNSSYVNREVQNPDRVFNRSRPNTPPSRSMESSSVGNNPSEKVWPEEQLRKKSLETIKEFYSAKDEMEVALCVKDLNAPSFYPSMISIWITDSFERKDMERGLLAKLLVNLAKSRDTLLSQPQLVEGFETVLACMEDAVTDAPKAPQFLGGIFAKVVLENVLPMAEIGRLIYEGGEEQGQLVEVGLAAEILGSVLEIIKTEKGEQVVKEICTVSGLHLENFRPPNSKKALTLDKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT64</accession>
    <name>A0AAF0WT64_DAUCS</name>
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            <fullName evidence="2">PGG domain-containing protein</fullName>
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        <name evidence="3" type="ORF">DCAR_0313395</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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    </dbReference>
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        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN NPR4-LIKE ISOFORM X1"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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            <begin position="162"/>
            <end position="182"/>
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            <begin position="157"/>
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        </location>
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        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="323" mass="36147" checksum="25087335A1FFFACE" modified="2024-05-29" version="1">MLNIIRVPSETGDKCQLLFVAAALGNTRFLVELLRLSPELLWKKDNNEHTIFHVAVLHRQENVYNLIYEIGSGKDIITSSRDSDGNSILHLAAMKPEQSRLHAVSGVALQMQRELLWFKEIETVVHPSLREMKNKQGKTPQDLFTEQHAELMEKGETWMKDTAAQCMVVAALIATMTFAAAFTLPGGTNGDNGQPIFKDRSAFIVFIITDAVSLFSSSASILMFLAILTARYAENDFLVSLPLKLMIGLLTLFISIATMMIVFSASFFLAYTKGVKWVPYLISFLAGLPVVMFAALLYPLFFSVVRSTYGSRHLFKPTKHVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVD2</accession>
    <name>A0AAF0WVD2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
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        <name evidence="9" type="ORF">DCAR_0416121</name>
        <name evidence="10" type="ORF">DCAR_0416125</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVD2"/>
    <dbReference type="KEGG" id="dcr:108217296"/>
    <dbReference type="KEGG" id="dcr:108217300"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GERANIOL 8-HYDROXYLASE-LIKE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
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    </dbReference>
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        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG96785.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG96789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56726" checksum="53C551D6EDC5532D" modified="2024-05-29" version="1">MDILITVTAFCILVAVTLVSVSRIRGKSHRKLPPGPCPLPIIGNIHKLGKHPHRSLAELAQVYGPIMRLKLGQITTIVISSSSMAQQVLQKQDAVFANRPVPESTRSCDHNKYSVVWLPVGSQWTSLRKILKSHLFTAKKLDENRHLRSRKVQELIRYCEKCSQSGEAVDIGRAAFLTTLNFLSNTIFSKDMTDSYDNSEAKEFKDLVWNIVVELGKPNLVDFFPFFKWINQTGKNQRIDGYSEKLIQLFDGLVNERLELKRSANFLENSRTTDTLDELLKLQQRNEIDKTQIQHLFMDLFVAGTDTTSSTVEWAMSEVLRNPETLFKAKAELNQVIGKGKIIGEEDISRLVYLRCILKETVRLHPPTPFLVPRQVKVEVELCGYTVPKNSQVLVSAWAIGRDPMLWKDPLSFQPERFMDSEINFTGHDYELIPFGAGRRMCPGMPLAMRMVPVMLGSLIHCFEWKLEGGIAPEELNMEEKVGLALAKLHPLRAVATSVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWG5</accession>
    <name>A0AAF0WWG5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0415060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47591:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
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        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG95733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="386" mass="42610" checksum="214E8C7FA49EB256" modified="2024-05-29" version="1">MLNNHATSHPPDNTPPNSDDQAMINRQFFRTGFRYDDDIDDDQNVPKKMMMMNPRKKRTKMVRIDNASTTASTSTDLVVAAIKPKYSKKPDPSAPKITRPCTECGRRFWSVKALFGHMRCHPERQWRGINPPPNLRRSSPIDHHRIAEDHVLLMNEEDQEVASCLLMLAKSPPPCTTIVSDQSNYPTSVTTNCTVGFSGLEIRNQNAVFGCGDVDNTVMDHGAGPSGVSSFKFECSSCKKVFGSHQALGGHRASHKNVKGCFAINKNLDCNAEEDYNINGGHDHRNVDYAGNPFGDQDKMLMVLGHRCSICLRVFPSGQALGGHKRCHWEKGDEPSSLTQGGPEPYQQFPQKGASALDLNLPVPLEDDYLSSSLNSGLSLDLSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZP1</accession>
    <name>A0AAF0WZP1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">DCAR_0417958</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR33827">
        <property type="entry name" value="PROTEIN SAWADEE HOMEODOMAIN HOMOLOG 2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33827:SF7">
        <property type="entry name" value="PROTEIN SAWADEE HOMEODOMAIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16719">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZY5</accession>
    <name>A0AAF0WZY5_DAUCS</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZY5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG97868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="23423" checksum="CF74271CD6A73294" modified="2024-05-29" version="1">MLFPLPFLHQEAPSPTDPVSWIENTYASSKTDFPLKKWSSIINEWIYPYKLYALMSMFFVIVLFPLPYSIDGPSERSLMLREIAMLASEQELLDFAEQVSMYSGCFHHRHQIVMSKKLLEDGTKTWNLVSEKNKQVLWENLAPEIEEQFKKISLNVRIYYHVYLVTTKIFLVEPVTKRIHVSSFNQLDQPLWAQFLQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X141</accession>
    <name>A0AAF0X141_DAUCS</name>
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        <recommendedName>
            <fullName evidence="8">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0417766</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X141"/>
    <dbReference type="KEGG" id="dcr:108217665"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000039">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF30">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042035549" description="Bulb-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="4">
        <location>
            <begin position="49"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="5">
        <location>
            <begin position="366"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG98423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50257" checksum="B703640EB354AD8E" modified="2024-05-29" version="1">MGVELVLSTCILSFLFFFVPSLNLISAQPFDYPTAKLSTSWINDPSVPPHTVHFGDGSIATPVLLRGTLGQRFACGFYCNGKCDSYLFAIFLVQTNSISHIVSPTVSFPQVVWSANRDHPVKINATLQLTSDGDLVLRDADGSVAWSTKTAGKSVAGLNLTDTGNLVLFNKRKEVVWQSFDHPTDALLPGQKLLEGQKLIASVSKTDSRAGVHSLVVNAKGLFAYIQSNPPQPYYELYVYGEEKTNKPNYVKFLNGSLDYYLHSSEPSAPTHTIYIPPASSEQYMRLESDGHLRVYEWKSKWEVSADVLTGFAGECTYPLACGKYSICANGQCSCPGASSSTVRFFRQIREKYPSLGCAEITPLSCSASKDQSFVELKDVAYFTFNSSIANTDVNKCKSDCLKSCSCKAAVFRYGSDSTKGECLLLSEVFSLQDHDSEQRHYNSTVYLKVQKNTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X178</accession>
    <name>A0AAF0X178_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Vta1/callose synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0519026</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X178"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="398"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="150"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="168"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG99672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="48801" checksum="ACE3CEC9885F5FC2" modified="2024-05-29" version="1">MSTGTENEPAKLLLPYLQRADELQKHDPLVAYYCRLYAMERGLKIPQTDRTKTTNSLLVSLIKQLEKDKKSLELTPDDSLHIEGFALNVFAKADKQDRAGRADKSTAKTFYAASIFFEILNQFGPLQPDLEQKQKYAAWKAADISKALKEGRAPVPGPPGGDNDLYSPVSSHYNTEPSNTDPVMKSANTDLGMNSAPEYNSSTQSYDRVDPQQSSTTAPLPPSNILTPPPSNPTPSPYPSNNFQSHSFHQPPANDYTSHNFHQPPLDDDYPSHNSHQPPSLISRSENNTYAQPYQQQPFPQEPQQHLPHYQSHDTSYTYPNFQTYPSFTESSLPAAPSHHPSYHQGPDTSYNITSASDASNYPSTAQQSSNGRNGTNLDPARASVQEFVYDSNYQPPPEKIAEAHKAARFAVGALAFDDVSIAVDYLKKSLELLTKPSGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X287</accession>
    <name>A0AAF0X287_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0518609</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X287"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041999351" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG99261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="395" mass="42817" checksum="73D5BCDE6282F55F" modified="2024-05-29" version="1">MAFSTSFLLWFFVTFSIAFPHSVVDAFVGTYGVNYGRLADNIPAPESVVTLLKAAKIRNVRIYDSDHSVLQAFKGSGIEIIIGLNNGLVKDVGASEDRAIAWVKENVEAFVPGTRIRGVAVGNEILGSGDQGLMEALLPAVKNVYSALERLNLAKEIEVSSPHSAAVFADSYPPSDGVFKDTVLPYMRPLLQFFSQIHSPFYMNIYPFLAYISDPVTIDIKYALFKKNKGVYDSKSKLHYDNMFDAQIDAAYAALDKAGFGKTEVIVSETGWASRGDANEVGASVKNAKTYNSNLRKKLLKKKGTPARPKTPVKAYVFALFNENLKPGPTSERNFGLFKADGSTAYDIGFTGLVPSSASSSFISIKGNGLQGLLGSSLSLMIYASCSVAVLLLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X458</accession>
    <name>A0AAF0X458_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0519289</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X458"/>
    <dbReference type="KEGG" id="dcr:108222106"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="18">
        <location>
            <begin position="365"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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            <tissue evidence="3">Leaf</tissue>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:140522"/>
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        </reaction>
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    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
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    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041772267" description="Pectinesterase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="560"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="204"/>
        </location>
    </feature>
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    </feature>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH01569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="560" mass="61522" checksum="5FA393C02350CFE8" modified="2024-05-29" version="1">MHFTTIAALLLTFSSLNLVTLQEKRSFPTDTKSLESSQSLHIRAASAACDGTLYSQLCVSTLSTFPNLRKKTLPQIISAAVNQTVVQVKATASNSSTIRRKIPHLDARDKRALQDCVELLNNTVTELNAAVSDLSSNKSSSKHYYDLQTLLSGAMTTTRKAAIFDRQNLYTCLDGFAYSDSNVRSYIEDSITEISRHVSNSLAMVTKIKEASRNEVFPGYGRVEGGHPMWLSREDKAMVDATTEVKYDLVVAKDGSGNFTTIGEAVAAAPDRSTKRFVIHVKAGAYYEYVEVESKKTMLMLVGDGIGKTLVKGNRSVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSGSDLSAFYQCSFVGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCALYARKPNENQKNIFTAQGREDPNQNTGISIINSKLAAAVDLIPVQSSFKSYIGRPWKLNSRTIIIRSNIGDLIEPAGWLEWDGDFALSTLYYGEYSNRGPGSNTSSRVTWPGYRVITNATEASQFSVGNFIQGGEWLNSTSVPFFLGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8Y7</accession>
    <name>A0AAF0X8Y7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0521474</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8Y7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF29">
        <property type="entry name" value="NODULIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="7">
        <location>
            <begin position="24"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="8">
        <location>
            <begin position="341"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH02086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="61456" checksum="CA60FC933E864550" modified="2024-05-29" version="1">MTENLSSAAKGSRLEFAFHVFRGRWLSLFASFTIMAGVGGTYIFGIYSKEIKASLGYDQSTLNLIGFFKDLGSNVGIFSGLIAEVTPTWFLLVIGSSLNFVGYFVIWLAVTGKIAKPKVWQMCTYMCIAANSLNFANTGVLVTCVKNFPENRGVLLGLLKGFAGLSGAIMTQIYLALYGNDPQSLIFLIAWLPAAISMAFVYTIRNISTGRQPNEVRVLYQFLYVSIVLALFLMAMTITQKLVTFPPVAYDASATIVCGLLFFPLFIVFREELFLWNQKKIPTADVRIEIPEQSTARSEEIPRTSCFSEICNKPPRGEDYTILQALLSVDMLILLISTACGIGSNLTAIDNLGQIGESLGYPMKNVNSFVSLVSIWNYFGRIFAGLMSEKLLIKYKFPRPLMTTIFLFLSCVGHLLIAFPVPGSVYVASVIIGFSFGAQFPLLNAIISELFGLKHYATLFNVGQLASPVGSYLLNVRITGVLYDHEALKDLSRKNLHRSSSGELTCIGAHCYRQSFIILAAAACFGAFASLILVLRTREFYKGDIYKKFRQETEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9R6</accession>
    <name>A0AAF0X9R6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallothionein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0522369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallothionein superfamily. Type 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9R6"/>
    <dbReference type="KEGG" id="dcr:108220108"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044671">
        <property type="entry name" value="MT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33357">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33357:SF3">
        <property type="entry name" value="METALLOTHIONEIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH02979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="6795" checksum="1EF7B987181EF3BF" modified="2024-05-29" version="1">MSNTCGNCDCADKTQCVKKGNSFGLDIVETEKISAETIMMEGSENDGKCKCGTSCTCTNCTCGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDB2</accession>
    <name>A0AAF0XDB2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0623625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDB2"/>
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        <location>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="470" mass="52770" checksum="F592CF870A133333" modified="2024-05-29" version="1">MYFFIITHMLDSKSLPKIPRDLTEASVSGAGMSIAAAFFMIFLFGMELNNYLMTTTSTSVIVDNSRDGDHLRINFNVSFPALSCEFASVNVNDVLGTNRLNITKEVHKTPIDTDLKASGSGYSTGPLLKVIRHDAEIDVEYTEGAVSLNAHNFERASHLYSILVVNFFAPWCSWSKRLRPSWERAAKIMRERYDPDVDGRILVAKVDCTEEAELCKRHNIQGYPSIRIFRKGSNMREPESYYGDRDTDSLITTMEDLVAPISADAQKIIFDLSKHPAPSTGGCRLEGFVHVKKVPGNLVVSARSPAHSFDATRMNMSHTISRFSFGKKITAEGFNDFKRVLPYLGRSHERLNKQSYITDRGNATAVNVTIEHYLQVVKTEVLTRAHKVVEDYEYTAHSSLVHSPALPVAKFHYEISPMQVRIEEVKKSFPHFLTNLCAIIGGVFTVAGILDSILHNTIRLIKKVELGKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDI9</accession>
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        </alternativeName>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="487" mass="53956" checksum="2132E9A695A092CB" modified="2024-05-29" version="1">MDNGLHERLLVPKAQEEKEIELKTKIWQESKNIWRVALPGVISRVGSFGTIVVTQSFIGHVSPLDLASYALVQTILVRFVNGILLGMSSATETLCGQAFGAGQYHMMGIYLQRSWIVDLITLTILLPVFIFGRQLFMLLGQEESIAKSGGYISLWFIPFVYSFVFSLTIQMYLQAQLKNKLIAWISTFQFILHVPLSWLLVSYFDFGVPGAMSALNISSWFLIFGEFIYIFGGWCPETWRGFSKAAFLDIFPVVKLSLSSGLMVCLELWYSAVLVLIAGYMENAEVAISAFSICLNINVWELMICLGFLGAACVRVANELGKGNADAVRFSIKVLIATSLVIGVFFSTICFLFHKEIAQFFTNDKEVADTVSDLSVLLGISVFLNSIYPVLSGVAVGAGLQATVAIVNLVCYYLIGVPVGIFLGYVVGLQVKGVWIGMICGVACQSITLCILAWRTDWDVQVQKAKERLNRFYLSSGEERHQSSNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XG52</accession>
    <name>A0AAF0XG52_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">Cytochrome c assembly protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071:SF1">
        <property type="entry name" value="CYTOCHROME B_B6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="HEME EXPORTER PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c assembly protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH05776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="82" mass="9518" checksum="876D955526E01E68" modified="2024-05-29" version="1">MLNIGRCYCIDDFWFDQYGVCSVRLQTRNWYSTVGFSVISLGFIFLTIGILSGAVWANEAWGSYWNWYPKIITYKLVNSGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="7">Hcy-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0727139</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
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            <name>Zn(2+)</name>
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        </cofactor>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Hcy-binding" evidence="7">
        <location>
            <begin position="9"/>
            <end position="323"/>
        </location>
    </feature>
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        <location>
            <position position="241"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
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        </location>
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        </ligand>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="35759" checksum="609A89ADF7973837" modified="2024-05-29" version="1">MGFESISAKLDDLIQKAGGCAVVDGGFATQLEIHGAAINDPLWSALCLIKEPHLIKQVHMEYLEAGADILVTSSYQATLPGFIARGISAKEAELLLEKSVNLAVQARNKFWDTVKDAPARSYYRALVAASVGSYGAYLADGSEYSGNYGPDVNLEKLKDFHRRRLQVLVNAGPDLIAFETIPNKLEAQACVELLEEENVNIPSWICFSSVDGVNAPSGESFQECLEVLNKSDKVAAVGINCAPPHFVQSLIQTFKKVTDKVIIVYPNSGEIWDGIAKKWMPSKCFDDDNFQAYAVRWRDAGAKLIGGCCRTTPSTIRSISKVLKEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH57</accession>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SRSF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="37"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH07888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="29457" checksum="CD264DCAA5785E0A" modified="2024-05-29" version="1">MRGRSYSPSPPRGFNRRGRSPSPRGRYNGGRSRDLPTSLLVRNLRHDCRAEDLRRPFEQFGNLKDIYLPRDYYSGEPRGFGFVQFVDPADAQEAKYQMDGQVLLGRQLTVVFAEENRKKPTDMRHREHRGGRFSDRRRSPPRYSRSPSPAPRYSGSRSRDYSPPHKRRQYSRSVSPEEKRYSRERSYSRSPVRERSPPYNGSRIRSHSLGRDASPNPPVARSPSRGRSPSRSRSRSPDPQHYSRGPGRDRSPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHY3</accession>
    <name>A0AAF0XHY3_DAUCS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
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                <person name="Senalik D."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="15">Leaf</tissue>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; Calvin cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNE1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="13">
        <location>
            <begin position="77"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="14">
        <location>
            <begin position="233"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH11020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="352" mass="38640" checksum="2082CA02D67BB79D" modified="2024-05-29" version="1">MITSTAPPSKLQYFSSSHSPSSLSPFQQCSLDTKAFAPRSSSAKKKQTLVSTTSIRCMAVETSKTTVQKKNAYEIQTLTSWLLKQEQGGGIDAELTIVLSSISLACKQIASLVQRASFFFNFTGGAVNVQGEDQKKLDVISNEKKSYSGNCIVVFDPLDGSSNIDAAVSTGSIFGIYHPNDECLTDYSDDDSLLDSEERKCVVSVCQPGSNLLAAGYCMYSSSVIFVLSIGNGKKIYSFNEGNYQMWDDKVKKYIDDLKDPGPTGKPYYARYIGSLVGDFHRTLLYGGIYGYPRDTKSKNGKLRLLYECAPMSFLVEQAGGKGSDDHSRVHQRVPLYIGSPEEVDKLEKYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNM9</accession>
    <name>A0AAF0XNM9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0830912</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNM9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000012">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000014">
        <property type="entry name" value="Aspartyl protease family protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF232">
        <property type="entry name" value="OS09G0542100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041958049" description="Peptidase A1 domain-containing protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="106"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH11426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="520" mass="57871" checksum="9DB81BA8CC9F590C" modified="2024-05-29" version="1">MCSFFCIFLLIFLSIWAIQSCNARIFTSDIHHRFSGLVKNWSESSRSNFFAGNWPEKGSVEYYFQLANHDHLLRYRRRRLSGSQDLPLTFSDGNATFRISSLGFLHYTMVSLGTPGMKFMVALDTGSDLFWVPCDCTQCAPIEDAPDDSDFELSIYSPVRSSTSKKVTCGNSFCTHQNSCGILNNCPYSVSYVSSETSTSGILMEDVLHFKSEDRKQEAVETYITFGCGQVQTGSFLDVAAPDGLFGLGLEKISVPSILSREGYTANSFSMCFRQDGNGRIDFGDKGSLDQEETPFDVAKFHPSYNITLTQIRVGTTLINLDFPALFDSGTSFTYLINPAYKRLTESFHSQIQDKRRAADPRIPFEYCYDMSPDKSSSLIPSMSLTLKGGGQLAVIDPIVIISTPRELVYCLAVIRSADLNIIGQNFMTGYRIVFNREDRILGWEKSDLEDSSSSHVLSRNSTTVPPAVAAGVGDRKTPDSATEAINRDKSSVASQLYIGYTSNLTKISFRFLVLLFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPD3</accession>
    <name>A0AAF0XPD3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Defensin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0831285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEFL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPD3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010851">
        <property type="entry name" value="DEFL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07333">
        <property type="entry name" value="SLR1-BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0295">Fungicide</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042010431" description="Defensin-like protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH11793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7928" checksum="BB66C3073AA4336A" modified="2024-05-29" version="1">MYNIYMCWCNHYIIILYCLMVLQGERGETCMAVKEGDCNVDDCVSWCSTTYNGNGTCTAINCTCMYPCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPF8</accession>
    <name>A0AAF0XPF8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA/RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0831357</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPF8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000225">
        <property type="entry name" value="Protein SMG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="3">
        <location>
            <begin position="70"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="2">
        <location>
            <begin position="203"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH11861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="990" mass="113016" checksum="739FB26DE088E73F" modified="2024-05-29" version="1">MNTEAHAVDEDDREKENNLLEVVSAERKLWALIHSKGLLRIDVQELYHKARSGYEDMIINDREGMELQDVEYCLWKLHYKHIDEFRKRIRHSSGSAENKKIGTSQNVASLQNNIEDHVEGFKTFLSDASLFYKDMIRKMRESCGLSEELLLSSKDYSSFSVVPANLLKCQYACHRFLVCLGDLARYKELCQKSENRNCKWSVAANYYFEAATVYPDSGNPHNQLALLATYVADEFLALYHCVRSLAIKEPFPDAWDNLMLLFEKNKSSPVHSLSSEIQYDISRPSERCFLRTKSQESSVSSNKKLEVTELASSGINDLWPLFVRMISFFFVKSRLEDFHCAFSSTLRKLEAVLALKETELKVSLESYGRLDAARSGPYRAIQLVSILIIIIHNLPEITKVNTSVDKNETEQSAFSKLAWTTTFIFMGRVIERCLEDMNGDSCPLLFTVLVFVEWLVGVLDKAELYSAHENVKNAAAYFFGVFCSLLNHLDNKEGEVKFLDRNAFWEDYELRGFSPVAHAHMQLDFSTQGEQIRNYDNGYTSRAHRILHAAMRIVEVSKTTRKWLSYDKVGRKFNAAEMIQSFDRREAEEMKVSHAKELKHDELYEKEISEENQDKPSVKNENASVDDEEVILFNPITRHNSEPIHSLLMSKGQMSLHEKDDQTASSDEVLRRATSLLLPQNQGQLESPKLYSGTTDFRFSKPYSQPEQTVIDSSAYPAGPPSLSGWVFNRESMHADSDKGPKAFHRHDLDPIAEMPSASLGGLSVNEKEFSPTTYNPSPLYVAPLPSAPQLPADANWLKGHSSNFQESKTGAKIREAEGILGPTPTTRYPNLSPTHGPFSPRLANFIDGYPPLLGMSSSEWLYQYRNSHIVEQANDRIWPVQVNAPGDFGNFYSHDASRFDFFDRWGNPLPSSPMVYLDNPHLNTNLPLYGVEEQRREKLFHGYQRPISYGCVTATDLGAEKPPLLQYLQEREWQLQRENKLRGPPYMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQ72</accession>
    <name>A0AAF0XQ72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0830421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH10944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQ72"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF49">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="105"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="174" mass="19785" checksum="BEAAF5F56DDB0ED0" modified="2024-05-29" version="1">MAPNVQRLFFKGIEERDQECLEIAGLKNNSKLLLRKSARNDRVEEVPASDDEIEVVKVTQKSKGSELVAEVKVDNDKLAAQVGALRKVICEGHKVFHKDLNILVEMLERQLLKLDGIKAEGEGKLQRKMEVRRVQGLLETADDLKRRNSVLIARSTESVADTTKCMPMFWPKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTH0</accession>
    <name>A0AAF0XTH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0933513</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the plant self-incompatibility (S1) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTH0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060320">
        <property type="term" value="P:rejection of self pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010264">
        <property type="entry name" value="Self-incomp_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31232:SF156">
        <property type="entry name" value="PLANT SELF-INCOMPATIBILITY PROTEIN S1 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05938">
        <property type="entry name" value="Self-incomp_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0713">Self-incompatibility</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="15959" checksum="A6D3FBD38403DDAF" modified="2024-05-29" version="1">MVRFTSATSIFCVFVYLLMTTCLSKFHINITNRLPNPPLILHCKSKDDDLGYHNLTINQLYTWSFRDNFWDTTLFWCNFWYNRKHAGFDVYNRHIRCDNTCYYEARIDGFYLSKTQEPGSWQKISPWNNSSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVC5</accession>
    <name>A0AAF0XVC5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small ribosomal subunit protein bS1c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">30S ribosomal protein S1, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0832605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVC5"/>
    <dbReference type="KEGG" id="dcr:108197251"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04465">
        <property type="entry name" value="S1_RPS1_repeat_ec2_hs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05692">
        <property type="entry name" value="S1_RPS1_repeat_hs4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000078">
        <property type="entry name" value="30S ribosomal protein S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000102">
        <property type="entry name" value="30S ribosomal protein S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000162">
        <property type="entry name" value="30S ribosomal protein S1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050437">
        <property type="entry name" value="Ribos_protein_bS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="102"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="190"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="267"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH13096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="416" mass="45595" checksum="0C6ED9FA120417AE" modified="2024-05-29" version="1">MAALAHQIGCGLKSPSLWPTHLSKKAIYKKNSSSQRPYIVKAAVTAPGVSVPTPQTRERARLKEMFEDAYERCRTNPMEGVAFNLEDFHHAIQEYDFNSEIGTKVTGTVFNTDANGALVDITAKSSAYLPVREVCIHDIKHVKEAGIVPGLREEFVIIGENSNDDSLILSLRSIQYDLAWERCRQLQAEDVVVKGKVVGANKGGVVALVEGIRGFVPFSQISTKSPAEELIEKEIPLKFVEVDEEQSRLVLSNRKAMADSQAQLGIGSVVIGTVQSLKPYGAFIDIGGINGLLHVSQISHDRVSDIATVFQPGDTLKVMILSHDRERGRVSLSTKKLEPTPGDMIRNPKLVFEKAEEMAQTFRQRIAQAEAMARADMLRFQPESTLTLTPDGILGPLSSDLPAIGLNLNDIPLAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AGK0</accession>
    <name>A0AAF1AGK0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0102258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG83985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="159" mass="17774" checksum="F9E4FF630CC69BA9" modified="2024-05-29" version="1">MAEQTEKAFLKQPKVFLCSKKSGKGKRPGKGGNRFYKSVGLGFKTPREAIEGTYIDKKCPFTGDVSIRGRILAGTCHSAKMMRTIIVRRNYLHYVKKYQRYEKRHSNIPAHISPCFRVKEGDHVIIGQCRPLSKTVRFNVLKVIPAGSSGGGKKAFTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMQ7</accession>
    <name>A0AAF1AMQ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Purple acid phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0208109</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="10"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMQ7"/>
    <dbReference type="KEGG" id="dcr:108209735"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45778:SF7">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45778">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041773491" description="Purple acid phosphatase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="14">
        <location>
            <begin position="144"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="12">
        <location>
            <begin position="277"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="13">
        <location>
            <begin position="511"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG88874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="652" mass="73962" checksum="7EB287644DD72ED5" modified="2024-05-29" version="1">MNTLCFVLNLFTIFFLLPSCLSKVSISFSPKTLSKSGDPVTIKWSGVDAPSELDWVGIYSPPNSSKDHYIGYFNLSKSETWQSGSGSLTFPLVNLRSNYQFRIFRWFESEVNPKHRDHDHNPIPGTKHLLVESEELGFEPGRGPEQVHLALTGQDGEMRVMFVTHDGKESFVKYGSSQDDMDRVVGTKVVRYEKEHMCDYPANHSIGWRDPGFIHDGVMKDLKKGKKYYYKVGSDSGGWSISYSFVSHNGDSNETVAFLFGDMGTATPYSTFYRTQEESLSTIKWISRDIDALGDQPAFISHIGDISYARGFAWIWDTFFNQIEHVASKLPYHVCIGNHEYDWPLQPWKPDWSYSVYGKDGGGECGVPYSLRFNMPGNSSEVAATNAPATQNLYYSFNVGVVHFLYISTETNFLQGSKQYNFIKHDLQSVNRDKFPFVVVQGHRPMYTTSNEDRELPLVKKMLEHLEPLFVENKVTLAFWGHVHRYERFCPLNNFTCGSFGYQGKSWEAFPVHVVIGMAGQDWQSIWQPRADHPTDPVFPQPKWSMYRGGEFGYTRLYATKEKLTLSYVGNHDGEVHDTVEILASGQVLDGNLDDDSAKARAETQSPLSWYIMGAGILVLGVFIGYVVGFILHSHRKAALGNKWMPVRSEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AN49</accession>
    <name>A0AAF1AN49_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0205346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AN49"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="114" mass="12410" checksum="32AB546B614BC7BA" modified="2024-05-29" version="1">MSSLESSTKQNSDDTNQASSQPKIITNEVLIDPVAQSMEQENDETKLANQVVEPMSRDNEKETENLEENAVKTKTTGVAKDVSAFGSVGPNFSLIMGQISLVDFFSEQGLNILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AP77</accession>
    <name>A0AAF1AP77_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0209663</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AP77"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042266674" description="2'-phosphotransferase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="302" mass="33776" checksum="E1AB26526B567680" modified="2024-05-29" version="1">MQVSSIFQFLVIFRTLIILIFLPVKCCIQTSNYLGGGSRGRSSNIRGSSRGRGGGRRGYTGRGACSPDKIDSLGRLMTWILRHTASKLNLEIRSDGYVKVQDLLKLDLYTLADIPLHSHTIADVREAVMADKKQRFSLMEENGELYIRANQGHSLKAVKTESLLIPIVSAEEVPVCVHGTYRENLQSILEHGLKRMKRLHIHFACGLPKDNNVISGMRQNVNTLIFLDVKKALEEGMKLYFSENKVVLTEGFNGVIPPKYFEKIESRPGRELLYVGKGEDLVTGMQDLTIEKGAKLQGTRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQ75</accession>
    <name>A0AAF1AQ75_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dof-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0208082</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQ75"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31089:SF52">
        <property type="entry name" value="CYCLIC DOF FACTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31089">
        <property type="entry name" value="CYCLIC DOF FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="10">
        <location>
            <begin position="96"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="451" mass="49390" checksum="BDF557814280B04E" modified="2024-05-29" version="1">MSEVKDPSIKLFGKTIQRFDALLHHDKLISSSKDPRTPDSSAEELIGQETSSGMTEDPKIPNDNKETVSLETDNKEDQSDLSNSDGKTLKKPDKLLPCPRCNSMDTKFCYYNNYNISQPRHFCKGCHRYWTAGGTMRNVPVGSGRRKSKSSTASLYRHILTSDAVQVARAEAANRINHPTASVLTFGSEPPLCESMASSLFLANKSQNCVKIGFNSDKQQTPVTYRNGENADDYSCGSSSTVPNPIEKGFSGGPESVLPTTVQGSPVHVPCFPMPPWPYPWNNAVQWRPQTPPTTGPSVYPVSFYPPPPYWGCMVPNSWNMPYPSQQPSSPNHPALRSSPNSLTLGKHSRDGGMLRPSQPGMGDPLNKKDPIRRLLVPKTLRINQSIGAAQSSKWPTLGIKTGNTDSGTGASLTNAFLPRATKKNHIMETSQVLQANPAAMTRSSNFHETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQH0</accession>
    <name>A0AAF1AQH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0208398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Senalik D."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="1">Belongs to the DEFL family.</text>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH00096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723:SF24">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH00096.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B1S4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0520116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <location>
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    </evidence>
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        <source>
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    <sequence length="299" mass="35447" checksum="846D32E9ECC815C8" modified="2024-05-29" version="1">MDGENRVKALVWVDPRSANAYKNFGDVVTFDSTYRTNRYCMPFIPITGVNHHYQNILFGFALIRDEKETSYEWVLKTWLEAVDNKPPITIITDQDIALGNAIAKILPTTKHTYCSWHIGQKFTEKLSSLYTHYPEFKTEFNACVYKSLSPEEFQERWDDLVEKYHLITHDWLNDMYNIREKWIRAYTKESFAAGMTTTGRSESMNSFFDQYVQPSTGLKEFIENSQKALEYQYLHEVEADYNTQYKKRRLILNSPLEAHASQIYTKEMFRRFQIEFVKSATREREYPITKIEDEEARIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the FLX family.</text>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="270" mass="30998" checksum="2E164573F4538D21" modified="2024-05-29" version="1">MEGRYRMPRHPEPYRGPRDDPRVLMHRGPGPLPPHPAALEEEIEYQHRDIQRLLSENRHVMDENIILQRDLAAVNDEMHRLGQVIHKLHAEKDAKTKDLIERGLKLESELRDIEPLRAEVVQLRAEAQKLNSVRRDMVTQVQHLTEDITRVTSENQQISAMRNDIDGMRKELTEARRAIEFEKKANEEQSEQKQLMEKNLITMAREVEKLRAEQLSLDRRGRGAGGYGILNGSPDTRYAGGDTRYASGAYGDVYGGGSWGVYDSRGSTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BF54</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="251"/>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BKA2</accession>
    <name>A0AAF1BKA2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="13">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0935515</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH15966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044813">
        <property type="entry name" value="ACP_chloroplastic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46153">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46153:SF20">
        <property type="entry name" value="ACYL CARRIER PROTEIN 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 13" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Carrier" evidence="14">
        <location>
            <begin position="58"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH15966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="139" mass="14509" checksum="A42DCE0BD16079DA" modified="2024-05-29" version="1">MASFTASSISFTPLSSSLNLNKGLAKSSVSLSINGISFPSHGLKSVSRRFSVSCAAKPETVDKVCEIVRKQLAIQPDVAVSGDSKFAALGADSLDTVEIVMGLEEEFGISVEEESAQTIATVQDAADLIENLVEKKGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BKX4</accession>
    <name>A0AAF1BKX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0935938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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    </dbReference>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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        <source>
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    <sequence length="207" mass="23650" checksum="D3BF1A781124B546" modified="2016-07-06" version="1">MGDRDIYINQSESESEADAKIPVDYFVTKDNINNKNKGLDAKRQLRFVDSSGNIVYTVNKTSSSASPGSVKTRLLLDASGNLLISLRRINSGSWQGFMRDANGEEDLIFRSERIQDTFNKTEFDLFLVHENCEQSRPDLKIKGRPFYRACTIYKGNSIVAQTSLMHKLWDAYVPRNKFRLTIFPGLVNRALVVALIVIYFDGRKIWL</sequence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Ashrafi H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YFH7"/>
    <dbReference type="Gramene" id="KZM91699">
        <property type="protein sequence ID" value="KZM91699"/>
        <property type="gene ID" value="DCAR_020936"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225281"/>
    <dbReference type="OMA" id="HIFRPDC"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF577">
        <property type="entry name" value="CARBOXYLESTERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="36248" checksum="4A5BBC03078C0EE6" modified="2016-07-06" version="1">MDNTFSVDPREAEVAHDIPPWIRVYKDGTVRRLSGYEVCDPGLDARTRVLSKDVMIVPGTGVSARLYRPSIIADGQRLPLVVYFPGGAYMISSPRFPGYHYSINNLVAEAKVVLVSVDYRKAPEHPLPIAFDDSWDALKWAASHASGAGSESWLINNVDFNKVFLAGDSAGASIAHQLMFRSKNMEIKAAGMKLVGMIMINPYFWGKEPIGAELTNTVRKALVDNWWAFVCPSDKGNDDPLVNPFAEGAPPLNDLGCARVLVCVAEQDILRDRGVLFYESLKKSEWKGEAKLIDVQGEDHVFHIFKPDCENAANMIKGLANFIHHQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YKG9</accession>
    <name>A0A161YKG9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0519593</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YKG9"/>
    <dbReference type="Gramene" id="KZM93899">
        <property type="protein sequence ID" value="KZM93899"/>
        <property type="gene ID" value="DCAR_017144"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223991"/>
    <dbReference type="OMA" id="SKWPPLR"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000009">
        <property type="entry name" value="Poly(A) RNA polymerase cid14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="9">
        <location>
            <begin position="105"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="8">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH00235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="522" mass="57599" checksum="6467945E4A4F29F3" modified="2016-07-06" version="1">MSIDESSSFLYETLTPLSTASPPPPSSEGYAVFRNEISLDSIVPSLDSKTSTTDYFSLDVGDDDRAASPPVEPVVTPAQSREPERTLEGNWFRSSSRFKSPMLQLHQEILDFCEFLSPTPEEEASRSAAVKSVFDVVKYIWPSCEVEVFGSFKTGLYLPSSDIDVVIMGSDIRSPQLGLMALSKALSQQGVAKKIQVIAKARVPIIKFVEKRSGISFDISFDVHNGPKAAEFIQGAISKWPPLRPLCLILKVFLQQRELNEVYSGGIGSYALLAMLIAMLRSVNDFHALPEHNLGVLLVSFFDIYGRKLNISDVGISCNGAEVFFFKSNKGFLNEGRSSLISIEDPQAPENDIGKNSFNYFQVRSAFAMAFSTLTNSKTILALGPNKSILGTIIRPDAVLLERKGNVEVTLNKLLPGAGDQLQPDDNDQEGLAFNWQLGNEEEPLPRGSGVSENGSMQSSGKKRKSSKVKKLGKKFKENDVELMLYEGSASRKEKSVKKKRWRHLRNSGERNSNGGDWSRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZS90</accession>
    <name>A0A161ZS90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_023207</name>
        <name evidence="11" type="ORF">DCAR_0622798</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM89430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZS90"/>
    <dbReference type="STRING" id="79200.A0A161ZS90"/>
    <dbReference type="Gramene" id="KZM89430">
        <property type="protein sequence ID" value="KZM89430"/>
        <property type="gene ID" value="DCAR_023207"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224878"/>
    <dbReference type="OMA" id="HCEWLNR"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd21669">
        <property type="entry name" value="SMP_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
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</entry>
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    <accession>A0A164T691</accession>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="Znf_LIM"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH09411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="24676" checksum="3F1317DE7B30E279" modified="2016-07-06" version="1">MSFTGTTDKCKACDKTVYFMDLLTVDGLTYHKSCFKCSHCKGTLAISSYSSMDGTLYCKPHFEQLFKESGNFSKNFHSTKEKENAVAHAPSKLSSLFTGTQDKCAACQKTVYPLEKITMEGESYHKPCFKCARGGCPLTHSSYAALDGVLYCKHHFAQLFLEKGNYHHVLEAAKFKNGAAAVDHTADHAEGAPEEKAEGTSEENAEDKPEEEAESTEDQPPEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VVV9</accession>
    <name>A0A164VVV9_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0624514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VVV9"/>
    <dbReference type="Gramene" id="KZM90937">
        <property type="protein sequence ID" value="KZM90937"/>
        <property type="gene ID" value="DCAR_021698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH05101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="319" mass="35460" checksum="AE13A754D7503B35" modified="2016-07-06" version="1">MDSEIVYNAIRDQDEVLFEENIEEVLRQINTIHANHFQVGVTSRRFSLTIPDMHNATVYLATQGLLRATSLVEVNLPFGDLQQIFDADMGLCLQLPALQIQPNLGLGEVVDAELPVETIVVSSDESSEAEDLVMEEEINNMHVLPPVPDVNPMANMPNVHVPNVFINLNVTVDVNWVRENVSIGQGNNVSPDTVQVIGEPLVHLNKGKGKLFANSVLSANGLLCQEAIRIIDSKELVFVSEEFDKDELDLEVQIVPGITTWDVLEHAVLGTLEPIIEAIAVRRGFEEQPRNGQNAMEVPLDLMDVEQVLMEMAMLEERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WEB5</accession>
    <name>A0A164WEB5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_020971</name>
        <name evidence="2" type="ORF">DCAR_0625364</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WEB5"/>
    <dbReference type="STRING" id="79200.A0A164WEB5"/>
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        <property type="protein sequence ID" value="KZM91664"/>
        <property type="gene ID" value="DCAR_020971"/>
    </dbReference>
    <dbReference type="OMA" id="NWCLASI"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF48">
        <property type="entry name" value="INOSITOL PHOSPHATASE SIW14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM91664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="75" mass="8734" checksum="F3860C1C0650EC75" modified="2016-07-06" version="1">MNFKELTILSGVSSDSLLQHRTGCAVGCLRKLQRWGLTSIFYEYQRFAAVKARVSDQRFMELFDVSTFKHIPKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XSJ5</accession>
    <name>A0A164XSJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_016783</name>
        <name evidence="9" type="ORF">DCAR_0519190</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XSJ5"/>
    <dbReference type="STRING" id="79200.A0A164XSJ5"/>
    <dbReference type="Gramene" id="KZM93538">
        <property type="protein sequence ID" value="KZM93538"/>
        <property type="gene ID" value="DCAR_016783"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220632"/>
    <dbReference type="OMA" id="WRERIKN"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22005">
        <property type="entry name" value="HMG-box_AtHMGB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031061">
        <property type="entry name" value="HMGB_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261:SF18">
        <property type="entry name" value="DNA-BINDING PROTEIN MNB1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
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    <dbReference type="AlphaFoldDB" id="A0A164ZSC1"/>
    <dbReference type="Gramene" id="KZM96333">
        <property type="protein sequence ID" value="KZM96333"/>
        <property type="gene ID" value="DCAR_019575"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220853"/>
    <dbReference type="OMA" id="RHESKHV"/>
    <dbReference type="OrthoDB" id="1934890at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33448:SF4">
        <property type="entry name" value="CHLOROPLAST PROTEIN HCF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33448">
        <property type="entry name" value="CHLOROPLAST PROTEIN HCF243-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="524" mass="58699" checksum="7E12A440DA5FC570" modified="2016-07-06" version="1">MEPSSSSDRATTHHRSHDLFICFTSTKTSTSSKSPTRQGSAMFNTASRKRGSGAESNPEPTSPKVTCIGQVRVKTKKQGDKMRNLSRRSAEMSFRKVMQRQSSFNSIHHQQECSSHRNQRWVHLPLTLCETLRSEFSCLFPCKSSCLSQSTTHCRDDLEEWMNDEEDYEDEKVVRRHVFDEIEIKDCFEQGGRVSLCVPPKNALLLMRCRSDPVKMEALAKKVYEPKLHHHEHDHDDEDCNDENDDEEEDCNDENDDGNVDVDHAVSFDKVKVVEVFEERRASISYNIVNQENLSDVKVNDLLISEGDDEICEPSYREEAQELKCAQDGAIEGEGDGQVLERDDLEIKNVEREGTQRLPECLLLMMCEPKLSMEVSKETWVCSTDFVRNRGQKKKLHAPNAPPCAKTGDGDGDAVVERRKSVDSRCGDNPFAALPPLYPAARDVQPGRASCCLPAARGGVSMASMIEQKLGDAVAYEPFVLTRCKSEPMRTASKLAQEGCFWKNKALGPHGRAPFGVGAAEAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WKP6</accession>
    <name>A0A165WKP6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0415631</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WKP6"/>
    <dbReference type="Gramene" id="KZM97463">
        <property type="protein sequence ID" value="KZM97463"/>
        <property type="gene ID" value="DCAR_015175"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217209"/>
    <dbReference type="OMA" id="EDERWAC"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG96296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="159" mass="17783" checksum="C78DB49C6B028138" modified="2016-07-06" version="1">MTFLNDHKFFFFAFGCAAFVVTMYHCLLSIFCHPRQHDFNDQPQQATGLGEVDIRSIPTHKYTTSNKSPKEECAVCLSEFEDGEDLRTLPKCLHTFHVVCVDLWLYSHTNCPTCRTDAAPEMHQLQQTGTINSSSSGGVAINLVSSRLSQDLEAQHAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WMK1</accession>
    <name>A0A165WMK1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_015134</name>
        <name evidence="4" type="ORF">DCAR_0415677</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WMK1"/>
    <dbReference type="Gramene" id="KZM97504">
        <property type="protein sequence ID" value="KZM97504"/>
        <property type="gene ID" value="DCAR_015134"/>
    </dbReference>
    <dbReference type="OMA" id="EANLEHW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF0">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="27"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM97504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="650" mass="73088" checksum="8FFC9F39C5F5C374" modified="2016-07-06" version="1">MSRSPSLSLKSVPNLSVDPKSFDQWAVAFCIIRFDLEQGQLIEDCYPPGCLSQEEELEVAFNSFPDSVSQHHNRSSIHDCIFFFRIKRRTSGVVLETSSKGRGKNTRYLYGFVFNRQRHDERLKRGGEQKSVVILSHSPYSSVFRPLLQIMGPLFFDIGKKALDNVASFVSKWPNPLPGQLMDLPIGNATLKVNLPPAHSLPLDGEVLFEESASSMAPLLPTNQSVPQGLFHDSDLFGTFKGLLLQLWVLWELLLIGEPILIIAPTPPQCCEAVASLVSLVAPLLLSVDFRPYFTIHDPAFAQLNSLQEGDVFPPMILGVTNLFFLKSLRNIPHTVSVGSPAPNSSRPSLAPRASIGSVPARLDAFGLQQSLKKFSPSSLLNAVKLRRDGPLCLMTEHKEAFWTTYTAITKPDTSVLNRLVDAGLSPRVEESMSVVNNEILRRHFLELTTNFLAPFGRYFRAFAPSEGVSPFVDPPPLPQFNDDDFLGSLSARGPGKFLSKRMRSNWLELYRRFLRGHNFRPWFERKRTVAEQEQHKLWRQARIKSDIYQYINRMSELEVVDSFNATERHLIQEEQASMLSGNADEDSLATCQKLRGDLKAVFNILPKDMHQLLLMNPERAALLQDTLEEGLREHPSAQAGLRNSSTPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A0I8</accession>
    <name>A0A166A0I8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oxalate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Acyl-activating enzyme 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Oxalyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_009369</name>
        <name evidence="14" type="ORF">DCAR_0310595</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A0I8"/>
    <dbReference type="STRING" id="79200.A0A166A0I8"/>
    <dbReference type="Gramene" id="KZN00615">
        <property type="protein sequence ID" value="KZN00615"/>
        <property type="gene ID" value="DCAR_009369"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214892"/>
    <dbReference type="OMA" id="LYHINGL"/>
    <dbReference type="UniPathway" id="UPA00372">
        <property type="reaction ID" value="UER00547"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050203">
        <property type="term" value="F:oxalate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033611">
        <property type="term" value="P:oxalate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05926">
        <property type="entry name" value="FACL_fum10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000039">
        <property type="entry name" value="Peroxisomal-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045310">
        <property type="entry name" value="Pcs60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="11">
        <location>
            <begin position="11"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="12">
        <location>
            <begin position="422"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZN00615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG91346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="521" mass="56419" checksum="1418CDB933CC13AD" modified="2016-07-06" version="1">MENLTLTALLKQVAAKYPDRRAISLSGQFDITHARLDQLIERAASLLVSADVKPADVVALTFPNTVEFIIMFLAVIRVRATAAPLNAAYTSDEFEFYLSDSESKLLLTSKQGNAPAQAAASKLKIPHITATLPAPNSEILLYPTQSESNSLSQIINESSDIALFLHTSGTTSRPKGVPLSQLNLASSVQNIKSVYKLTESDSTVIVLPLFHVHGLIAGLLSSLVAGASVTLPAAGRFSASTFWGDMNKYNATWYTAVPTIHQIILDRHLNNPEPRYPNLRFIRSCSAALAPSIMERLEEAFKAPVLEAYAMTEACHLMASNPLPEDGPHIPGSVGKPVGQEMAILDENGVAQEAGAKGEVCIRGPNVTKGYKNNPEANRTAFQFGWFHTGDIGYLDSTGYLHLVGRIKELINRGGEKISPIEVDAVLLSHPEISQAVCFGIPDDKYGEEINCAVILKEGSEIDEAEVLNFCKKNLAAFKVPKKVFITDSVPKTATGKIQRRIVAEHFLSQISTAKVPKFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ARD5</accession>
    <name>A0A166ARD5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF3527 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_010433</name>
        <name evidence="3" type="ORF">DCAR_0311832</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ARD5"/>
    <dbReference type="STRING" id="79200.A0A166ARD5"/>
    <dbReference type="Gramene" id="KZN01679">
        <property type="protein sequence ID" value="KZN01679"/>
        <property type="gene ID" value="DCAR_010433"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211544"/>
    <dbReference type="OMA" id="NQCAFSN"/>
    <dbReference type="OrthoDB" id="1898655at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021916">
        <property type="entry name" value="DUF3527"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31390">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31390:SF12">
        <property type="entry name" value="PUTATIVE (DUF3527)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12043">
        <property type="entry name" value="DUF3527"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN01679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="809" mass="88421" checksum="C42C02542BAA49F6" modified="2016-07-06" version="1">MEYNVELKKSLSQHANCDQIPCQSGTNKDDELVKHMSNLPAYLQQMERGETVQEKPLNFGVLDWNRLEKWKYNEKPVRARATKKVSSNNSPSVISGTAKKQTNAGQRRQVSPQNWRPNSAFEEGQGVGARRSVTKPRGHETSSRSSLDMIQKLQPCEKFPGTKYSEVSLEARKLIESVCNAVSERVAQSSSSGEDQLAGFLIDEDTEINSVKEEFDLATQASGNANIVLILPKSVSSRTFSEDMKLSKSRNFYDGNLAVAVGRRFSSSSSPNKVHSGETYSEVPHSCPLLTSDATKVDSDAEQKDFVKPQGIEQYDESHTHQRLTKITIPKSDIEPLITVTPKRSVQSLAAGPAAKGRQSSPRQRFSFSSIGRMTKSFSFKEGLAVPQLNSTYTTVKSGPVGSLAFADPDKFNQNNGSTGNRSRSSPLRRLLDPLIKPKGVHSAEFVKPAKIDLDPLKANSMDTTELLHCQTGKTSCVQALLELTLKNGLPLFKLVVNNSSDIFAAAVKKLPTPEEDVPSLIYALYSVHEIKKKSGNWMSQGSKGISSHLSYNVIGQINVARSRLPGIGCQNTNDKCTVRESILYGVDIEQENTETPKFIPGKELAAVVIDNTNDTSDGEQSEKSSHCQSNNFDGEVRGYSNSTTVILPGGVHGLPNRGAPSLLMNRWRSGGSCDCGGWDVGCKLHILTSQNQTSKNLSTLPSSVVEGEGLNLFVQDGHKDNKLIFSLAPFTGGVYSVDFDSSISLLQAFFIAVAFLSSQKLSNLFAVEMEKENLAEPTNVMIKIPTPIQQEAPTRYVINPPPSPAGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AT97</accession>
    <name>A0A166AT97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_010523</name>
        <name evidence="14" type="ORF">DCAR_0311963</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AT97"/>
    <dbReference type="Gramene" id="KZN01769">
        <property type="protein sequence ID" value="KZN01769"/>
        <property type="gene ID" value="DCAR_010523"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007870833" description="ABC transporter domain-containing protein" evidence="10">
        <location>
            <begin position="33"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Guanine nucleotide-binding protein-like 3 N-terminal" evidence="12">
        <location>
            <begin position="35"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="11">
        <location>
            <begin position="155"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZN01769.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG92688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="49361" checksum="53AE688AB82C2BBB" modified="2016-07-06" version="1">MTGQLKIHFVRSVLQSLCVYQLCLCGTPSVDGSSQKCDTKDAKKQGLNKRPKVEKVPSIPNDWPFKEQELKALKARLSLALEELEQKKAAWKGKGQKEEIAPAKDDNVDAWKEQNAGEEIVTAKTAPIVKIRGVTHLLMHVNLHRYNYSWDDYKTAKLVVSAPITMTVFLRANTSINIVNANYFPMSLFYSLIRFNSNAILELSMIVSRLAIIYKQRDFNFYPAWAYSIPAVILKIPFSFVDAFQWTGLTYYVIGLFQLGVLTALMGIIGAEKTTLLDVLFGRDIRIGGYPKVQDTYARISGDCEQTDIHFPQITVNESLTYLSWLRLPPHIDDSTKATKKKPHRSSFTGIFAAAFGSGIQFFAMELLRQTKVKNTINETELNSDENIFFDLFIKPNCGDTLPDCELLDMAKDWINREPQEVLLGWEVHIRGMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B6T0</accession>
    <name>A0A166B6T0_DAUCS</name>
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        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0312646</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_011180"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214548"/>
    <dbReference type="OMA" id="LTMEDTI"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
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    </dbReference>
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        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000126">
        <property type="entry name" value="Putative ABC transporter B family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136:SF2">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG93363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1243" mass="135115" checksum="274A6229564AF6BF" modified="2016-07-06" version="1">MAKTVLKKKTTGSIRSIFMHADGADKLLMTLGFIGTVGDGFSMPVMLYTTSMLMNDIGGTSSGVDAQFTHSINKNALSLCYLALGQLVVCFFEGYCWTRTAERQASRLRARYLKAVLRQDVGYFDLHVTSISEVITSVSGDSLVIQDVISEKIPVFVMNVASFIGAYVAAFLMLWRLAIVGLPFLVILIIPGLIYGRTLMGIARKMREEYNKAGTIVEQAISSIRTVYSFVGESKTITEYSAALEGTVDLGLKQGLAKGLAIGSNGLVFAIWAFMSFYGSRLVMYHGAHGGTVFAVGAATAIGGLSLGAALSNMKYFSEATAAGERILEVINRIPKIDSDNMEGEILENVSGEVEFKHIEFAYPSRPETIIFKDLNLKIPAGKAVALVGGSGSGKSTVIALLQRFYDPVGGEIMVDGVGIEKLQLKWLRSQMGLVSQEPALFATTIKDNILFGKEDGSMEEVIGAAKAANAHNFISQLPQGYDTQVGERGVQMSGGQKQRIAIARAIIKAPRILLLDEATSALDSESERVVQEALDKASVGRTTIIIAHRLSTIHNADLIAVVQDGEIRETGSHNDLIQDPNSLYSILVHLQHSQKNCVPLSTSTNYKVASISNVDINNTSSRRISMVSRSSSANSRGGEYVNLDNKHPTEQIFPVPSFKRLLAMNLPEWRQAILGSCGAILFGAIQPVYAFAMGSMISVYFLPEHDEIKEKTRIYALCFVGLAVFSFLFNIIQHYNFAAMGEYLTKRIRERMLSKILTFEIGWFDQDENSSGAVCSRLSKDANVVRSLVGDRMALVVQTLSAVIIACTMGLVIAWKLALLMIAVQPLVIVCFYCKRILLKNMSSKAIKSQDECSKLAAEAVSNLRTVTAFSSQSRILKMLEKAQEAPRRESARQSWYAGIGLGVSQSLMSCTWALDFWYGGKLIADGEISSKALFETFMILVSTGRVIADAGAMTNDLAKGSDAVGSVFAVLDRYTLIEPEDPDGHKPEKVMGHVELRDVDFSYPARPDVPIFAGFTIKIEAGKSTALVGQSGSGKSTIIGLIQRFYDPLNGSVKIDGKDIRSFHLRSLRKYIALVSQEPTLFAGTIRENITYGASEETDEIEIIEAAKAANAHDFITGLKDGYETWCGDRGVQLSGGQKQRIAIARAILKNPAVLLLDEATSALDSQSEKVVQDALEHVMVGRTSVVVAHRLSTIQNCDTIAVLDKGKVVENGTHSALLAKGSTGAYYALVNLQRRADTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D076</accession>
    <name>A0A166D076_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor</fullName>
            <shortName evidence="5">bHLH transcription factor</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Basic helix-loop-helix protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0206050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D076"/>
    <dbReference type="Gramene" id="KZN04559">
        <property type="protein sequence ID" value="KZN04559"/>
        <property type="gene ID" value="DCAR_005396"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210106"/>
    <dbReference type="OMA" id="DAEAPCK"/>
    <dbReference type="OrthoDB" id="677168at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11449">
        <property type="entry name" value="bHLH_AtAIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045084">
        <property type="entry name" value="AIB/MYC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11514">
        <property type="entry name" value="MYC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11514:SF47">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG86831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="591" mass="65954" checksum="F700F66626F406F5" modified="2016-07-06" version="1">MGESMWTEEDKDMAVAVLGNCAFDYLISSSVSTDCSFMAVGNDENLQNKLSDLVECPDLSSFCWNYAIFWQVLRDKSGEVVLGWGDGSCRELREGEESEVDGILNLRCEDESLQRMRKRVHQKLHSLFGGSDDECHSIGLDRVTDIEMFFLASMYFSFPHGEGAPGKCFRSRKHVWISDALKSPSDYCFRSFLAKNAGIQTLVLIPTDVGVVELGSVRTIPENIELVQSIISAFSSSPSVCKSNIPTVAPLMREKGDGKTQFLDGGVVERPRITKIFGQDINSNRSELRPKLAVRKPDERPWDVYANGMKPQFMTTRNDNHGLHGTQFTNGKQGNTVEIYSHPQTPTSNIFKRGAHEEFGFKKFKTQIPAQVNIDFSGDKTRPSQPVIIESEYSDVDAAYKEEQAALADDKRPRKRGRKPANGREEPLNHVEAERKRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYITELQKKVKDLESIGSTSRDASSSDANPNGIPTPEFHNQVSNIEIQAADDIVNVRVTCPLESHPASRVIEAFREAKVSVVESKLAAAKDTVFHTFVLKTEGTEQLTKEKLLAAFSREANSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DHK9</accession>
    <name>A0A166DHK9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_006105</name>
        <name evidence="2" type="ORF">DCAR_0206864</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DHK9"/>
    <dbReference type="STRING" id="79200.A0A166DHK9"/>
    <dbReference type="Gramene" id="KZN05268">
        <property type="protein sequence ID" value="KZN05268"/>
        <property type="gene ID" value="DCAR_006105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN05268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="112" mass="12656" checksum="98C3C25FAE317BE5" modified="2016-07-06" version="1">MLYKLVLLTYFYMSVMIEKQRIYFSSRRPQGPRATVIVMSDQARGMHHPILGEVDNKRAYQAAEVIAAVNKINRSTIVPEYSFSDARGPYLALAPELAISRKKSKTISSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DTY0</accession>
    <name>A0A166DTY0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-3' DNA helicase ZGRF1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_006511</name>
        <name evidence="3" type="ORF">DCAR_0207337</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DTY0"/>
    <dbReference type="Gramene" id="KZN05674">
        <property type="protein sequence ID" value="KZN05674"/>
        <property type="gene ID" value="DCAR_006511"/>
    </dbReference>
    <dbReference type="OMA" id="KYHSGIL"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052800">
        <property type="entry name" value="DNA_Repair_Helicase_ZGRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018838">
        <property type="entry name" value="ZGRF1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535:SF1">
        <property type="entry name" value="PROTEIN ZGRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535">
        <property type="entry name" value="ZINC FINGER GRF-TYPE CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10382">
        <property type="entry name" value="ZGRF1-like_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="1">
        <location>
            <begin position="134"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="1">
        <location>
            <begin position="231"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN05674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="581" mass="64925" checksum="BE62F53BCCFC9CDF" modified="2016-07-06" version="1">MQSMLSITCFGLSESSFLSYFLDSEWQVLYTAQLTQKAKKFHDGILKLSVCGSQRRQVFLYDETGRLLDSRFLKSDENIRSGESLKLDGHLVDIGNLNGDNEPHEESKLQGGDCVVVKETGRHHQQVQEQTNFHEWQVLYTTQLTQKAKKYHDGILKLFVYGSQKRQVFLYNESGGLLDSRFLKSDENVKSGESLKLDSHLVNIGEPKTDNETVEESKYPGGDRIVSGETEWDAMYTAQVTQRNKKYHSGILKLASCGSYRMQATLLAEDGTTLCRRYLKLSEHVSSGSEFQFPSYLVEVGEPRKHHDELQSKDSPCEESDSLRCSRVEKTKESMEICFAGVQSKELQWKKPGSVVRSSKDEIKPSGETSVEKPVRDVHGILSFLKKPVNQECSVSVKREFSEEGHALQDLDIFHIEIQNSVQGQLVDDSRGQCSALEDSNGDIADIISSDAREDPESKILASAVSLCHGNKQKPTSSTFTSQIVDDDSGFQQPIVSAMPNGLSAKDNINNHQESVFSQAASRFGFGQPLKEVKRKKLSQLSVDSLGINSRCNESSDPCNVDGEKFSTVVTDEFPSFDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DWI4</accession>
    <name>A0A166DWI4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myelin-associated oligodendrocyte basic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_006611</name>
        <name evidence="4" type="ORF">DCAR_0207444</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DWI4"/>
    <dbReference type="STRING" id="79200.A0A166DWI4"/>
    <dbReference type="Gramene" id="KZN05774">
        <property type="protein sequence ID" value="KZN05774"/>
        <property type="gene ID" value="DCAR_006611"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205697"/>
    <dbReference type="OMA" id="IEVLWTP"/>
    <dbReference type="OrthoDB" id="542507at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN05774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG88210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="42230" checksum="8D032F7C578FCF15" modified="2016-07-06" version="1">MVTPFLLESNLKWNPLLHTFPIHYSPIKPTNHFIKHLVKYNQTPKFHIKCSSSPSFNNIPTRAVSNSPNKQNPVQILAQSIIRTLKSLQKPAITAVLLGILLLSDPTNVLAASGGRMGGKSFSRPSVSSSRSYSAPRMDSGRSFSSAPYFGPSPFGGGGFYVGPAVGIGSSFFFIMAGFAAFVLVSGFLSDRSDDGLLTATEKTSVLKLQVGLLGMARSLQQDLNRIAERADTSTPEGLSYVLTETMLALLRHPDFCISAYSSVDVKRSMDEGEKRFNQLSIEERGKFDEETLINVNNIKKQSATSRRANGFSNEYIVITILVAAEGVHKLPSINNSGDLKEALQVLASIPSSRTLAVEVLWTPQNENDTLSERELLEDYSLLRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DXE8</accession>
    <name>A0A166DXE8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_006637</name>
        <name evidence="4" type="ORF">DCAR_0207475</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DXE8"/>
    <dbReference type="Gramene" id="KZN05800">
        <property type="protein sequence ID" value="KZN05800"/>
        <property type="gene ID" value="DCAR_006637"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872418" evidence="2">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN05800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG88241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="81" mass="9005" checksum="CDF5419AF7B69564" modified="2016-07-06" version="1">MASHHKIVTVFLIMLVAFSFVVSIEAAEESKETFGSLNPFKKAPGKNFMPGPQKEEINTEAVGHMNPFKKDARKEQESPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DY98</accession>
    <name>A0A166DY98_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0207504</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DY98"/>
    <dbReference type="Gramene" id="KZN05827">
        <property type="protein sequence ID" value="KZN05827"/>
        <property type="gene ID" value="DCAR_006664"/>
    </dbReference>
    <dbReference type="OMA" id="MKTTMPM"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF40">
        <property type="entry name" value="SHAGGY-RELATED PROTEIN KINASE DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="422" mass="48062" checksum="28EF8C3FF9B92488" modified="2016-07-06" version="1">MASIGHRKVGSSRTVNGFNESSSSVDWLGREMLEMRLRDKDKVEHDEERDSEPEVVNGVGAEAGHVIRTTIGGRNGQSRQAISYIAEHVVGTGSFGVVCQAKCRETGEILAIKKVLQDKRYKNRELQIMQMLDHPNIVALKHSFFSTTDKEEVYLNLVLEFVPETVNRMARHYSRLNQRMPLIYVKLYTYQICRALAYIHNCIGICHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVKGEPNVSYICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKPHPWHKVFQKHLPPEAVDLVCRFFQYSPNLRCTALEACIHPFFDELRNPDTRLPNGRPLPPLFNFKAPELAGIPAEILRRLIPDHARKQNSFMALHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EBH0</accession>
    <name>A0A166EBH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_007175</name>
        <name evidence="18" type="ORF">DCAR_0208097</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EBH0"/>
    <dbReference type="Gramene" id="KZN06338">
        <property type="protein sequence ID" value="KZN06338"/>
        <property type="gene ID" value="DCAR_007175"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210153"/>
    <dbReference type="OMA" id="SDHHWSL"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000355">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="155"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KZN06338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG88862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="440" mass="49346" checksum="4F77C2734298CB2E" modified="2016-07-06" version="1">MSESGGSSGHSAAGDAAPDAKKQSKAKVSRTSLILWHAHHNDESAVRKLLEEDPKLVHAIDYDNRTPLHVASLHGWIDVAKCLIEFGADVNSQDRWKNTPLADAEGAKKHGMIELLKSHGGLSYGQSGSHFEPKPVPPPLPQKCDWEIDPSELDFTHSAMIGKGSFGEIIKAGWRGTPVAVKRILPSLSDDRLVIQDFRHEVNLLVKLRHPNIVQFLGAVTDRKPLMLITEFLRGGDLHQYLKGKGALSPSTAVSFALDIARGIAYLHNEPNVIIHRDLKPRNVLLVNSSADHLKVGDFGLSKLIKVKNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVYSFAMILYEMLEGDPPLSHHEPYEAAKYAAEGHRPMFRAKGYTQELRELTEHCWAADMNKRPSFLDILKRLEKIKETLPSDHHWKIFTSSHVKHDPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FXQ0</accession>
    <name>A0A166FXQ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_000851</name>
        <name evidence="12" type="ORF">DCAR_0100807</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FXQ0"/>
    <dbReference type="STRING" id="79200.A0A166FXQ0"/>
    <dbReference type="Gramene" id="KZN08305">
        <property type="protein sequence ID" value="KZN08305"/>
        <property type="gene ID" value="DCAR_000851"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198954"/>
    <dbReference type="OMA" id="VFPRKFN"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000126">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951:SF3">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951">
        <property type="entry name" value="OS08G0547900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN08305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG81656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59163" checksum="8D9745E3A2468FEA" modified="2016-07-06" version="1">MYQENLINVSDAWLWLGNFGNTNIRACILMTILVFISWFAVFIKSKKTSHPLPPGPRGLPLVGNFLSLDADNLHICFANLAKTYGPVMKLQLGRKACIVITSPSLACEVFKEQEINFINREVTAAGFEASYGGTDIVWTPYGPEWRMLRKVCVHEMLSNTALDSVYSLRRREIRRTVQYIYSQIGSPVNVGEHMFLTIINVITNMMWGSTVKGEERVVIGTKFRQVVNEITQLLGMPNVSDFHPGLARYDFQGIQKKMKDLVKRFDDIFETVISEKQKTGQEGIRDFMECLLKFKSDPDAKIPFTMTHIKALLMDMVVGGSESTSNTMEFALAELINKPQLMQKVQEELQTVVGDDDMVEESHIHKLPYLYAVMKETLRLHAPIPLMVPRCPTETSVVGGYTIPKGARVFINVWSIHRDPSIWERPLEFDPERFLDDKWDHSGKNFSYLPFGSGRRICAGITMAERMFLLSLASLVHSFNWNLPQGMKIDLSEKFGIVLKKRIPLVAIPTPKLSKLELYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G3Q6</accession>
    <name>A0A166G3Q6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0101000</name>
        <name evidence="2" type="ORF">DCAR_032175</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166G3Q6"/>
    <dbReference type="STRING" id="79200.A0A166G3Q6"/>
    <dbReference type="Gramene" id="KZN08501">
        <property type="protein sequence ID" value="KZN08501"/>
        <property type="gene ID" value="DCAR_032175"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198087"/>
    <dbReference type="OMA" id="CTARANN"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053232">
        <property type="entry name" value="DnaJ_C/III_chloroplastic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45090">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45090:SF3">
        <property type="entry name" value="OS09G0368800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="49"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG81846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="165" mass="19102" checksum="CAA0BD9E361D9C2E" modified="2016-07-06" version="1">MEISLKLNIDTVKSVPYLYKSKTVSSSGFKVNKVSCRVKEMGTKQERANFYELLSLNSKENVGFEEIKKAYRSKALEYHPDVCPPSNREESTRKFVEIRKAYDTLSDPASRQMYDYEMSLVDSFGGSYLYEGKRDGFSKKVWERQLSGLRKRSLDKSEKKNSFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H318</accession>
    <name>A0A166H318_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_002315</name>
        <name evidence="18" type="ORF">DCAR_0102402</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H318"/>
    <dbReference type="STRING" id="79200.A0A166H318"/>
    <dbReference type="Gramene" id="KZN09659">
        <property type="protein sequence ID" value="KZN09659"/>
        <property type="gene ID" value="DCAR_002315"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204855"/>
    <dbReference type="OMA" id="AKFYKMM"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000164">
        <property type="entry name" value="Casein kinase 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="299"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KZN09659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG83227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="465" mass="52628" checksum="B5B5860752D5B6EE" modified="2016-07-06" version="1">MDHVVGGKFKLGRKIGSGSFGELYLGVNVQTGEEVAVKLESAKTKHPQLHYESKLYMLLQGGTGIPHLKWFGVEGEYNAMVIDLLGPSLEDLFNYCNRKFTLKTVLMLADQLINRVEYMHSRGFLHRDIKPDNFLMGLGRKANQVYVIDYGLAKKFRDLQTHKHIPYRENKNLTGTARYASVNTHLGVEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGTKKQKYDRISEKKMLTPVEVLCKSYPSEFISYFHYCRSLRFDDKPDYSYMKRLFRDLFIREGYQFDYVFDWTILKYPQIGSSSKSRQPSGKLPLNPGPTIDRAEKTPVKREMRDRLSGAVEAFARRKGSSSGANLRGDHLRHRSSDNVPSSKDMQAEPVRGRVSRAASASKRAVASSSRPSSSGEPSENRLNRLGSGSGRLSTTQRIQPGFEPKPSSFTRALPSRGGRDDALRSFELLTIGSGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HT82</accession>
    <name>A0A166HT82_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0103174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HT82"/>
    <dbReference type="Gramene" id="KZN10362">
        <property type="protein sequence ID" value="KZN10362"/>
        <property type="gene ID" value="DCAR_003018"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207352"/>
    <dbReference type="OMA" id="VHGLSWH"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="14554" checksum="6BC7B2FE9FDB7356" modified="2016-07-06" version="1">MAFFNKIGSLVSQTNLKTSVPSMFNTIRGMASSKLFVGGLSYQTSDDSLRDAFSSFGDVTEARVIFDRDTQRSRGFGFVSFADSESASSAKSAMDGQELEGRNIRVSEATERPPRENFGGGGGYRGGYGAGGGNDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IDA0</accession>
    <name>A0A166IDA0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IDA0"/>
    <dbReference type="Gramene" id="KZN11008">
        <property type="protein sequence ID" value="KZN11008"/>
        <property type="gene ID" value="DCAR_003664"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="6208" checksum="1C12169A393BCBFC" modified="2016-07-06" version="1">MSLDYSFTSHHAWTDHISDAIHRPPNMDTASLTKAIDDPVLIKAARETPLNALFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IFJ7</accession>
    <name>A0A166IFJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-3-methyladenine glycosylase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_003735</name>
        <name evidence="4" type="ORF">DCAR_0103967</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IFJ7"/>
    <dbReference type="Gramene" id="KZN11079">
        <property type="protein sequence ID" value="KZN11079"/>
        <property type="gene ID" value="DCAR_003735"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205416"/>
    <dbReference type="OMA" id="PRQPAIM"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116:SF4">
        <property type="entry name" value="DNA GLYCOSYLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116">
        <property type="entry name" value="OS04G0501200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="365"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        </location>
        <ligand>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN11079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG84782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="43514" checksum="04E4C81B39454D05" modified="2016-07-06" version="1">MCSSKSKLQQTTTPSFDLNGRPVLQPNQVPLLERRISSKKSVTPPKIQPLRPPHSNVTSPKLRPFVTTPPMSPKLKSPRQPAIKRGNDPNGLNSSVEKLVLTPKSDPKARIPVKKSKTETACVDTSTLNYVSSCIVEAPGSIAAARREQVANLQVQRKMKIAHYGRSKSAKYDPYLNLNDNIEPTLNVTEDKRCSFITPNSDPIYVAYHDEEWGVPTHDDKVLFELLVLTGAQVGSDWTSVLKKRQEFRDAFSEFDAEIVSKYSEKKITSISAEYNIELRLVRGVVDNSNRILQIKKEFGSFDKYLWGFVNNNPIVTQYKLCNKMPVKTSKSEGISKDMVKRGFRLVGPTVLHSFMQAAGLTIDHLITCPRHLQCTALAQRRATVAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166JAJ9</accession>
    <name>A0A166JAJ9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0105014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166JAJ9"/>
    <dbReference type="Gramene" id="KZN11966">
        <property type="protein sequence ID" value="KZN11966"/>
        <property type="gene ID" value="DCAR_004622"/>
    </dbReference>
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    <dbReference type="OMA" id="VEMWSEV"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
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        <property type="entry name" value="Beta_prop_At2g24240"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
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    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF23">
        <property type="entry name" value="PROTEIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25279">
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    </dbReference>
    <dbReference type="Pfam" id="PF02214">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="469" mass="51027" checksum="4B4ACFC966BE380D" modified="2016-07-06" version="1">MPSFASSPPPSHGFHPKSRDFKSDVCTIDVGGQLFQTTKQTLALAGPNSMLYQACEAGDEAVPFIDRDPELFAILLSLLRTGNLSSKARGIDVQDLIFEAQFYGIEDVLVASQSNPSQFEAFNLEKSLLLSLNGRDSPNCMSTTPYGSVHVAHGSKITSFDWSLRKKSTILTEFTAIDSLLSISPVIAAAGATDFSGLQILDLDKGFVRETLSWENVTRSSSTVQAIGSSPDFLFSSFESGRRNSSAIMVYDLQGGFRPVAEIGHCEVYGADLGSAIPATKLNWVPSYNLLMASGSHSGPQGVMGNIKFWDIRSGNVVWELKENVDCFADVTVMDNLSAIFKVGVNSGEVFFSDMRNMGAENSWECLGDKRKAASGKKEGVGCIIQSHNNQVFCSKGGDLELWSEVMVGSLRKTDNGTGGRVFRKNLMGREKDMGGSRITNLQFGGNKMFVTRKNQQFVEVWQSSVRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YB09</accession>
    <name>A0A175YB09_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Zheng Z."/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_026494"/>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </location>
    </feature>
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    </evidence>
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    <sequence length="468" mass="51251" checksum="41AC54BD63B784F3" modified="2024-05-29" version="1">MDKKQGFFTALREEVVRGLSPRRSRDRTPGRVGSPLSGLLRRRKASSRSSNLGISENVISRSGSMRTLTPLMEGPDPDGGEIGDSKRVGLGQWITGQLTRTPSSLNASFDNKKSDLRLLLGVMGAPLAPVYVNATDPLPHLSIKDTPIETSSAQYILQQYTAASGGQKVQNSIRNAYAMGKVKMLASEFETATRVMKNKNATRDAESGGFVLWQMNPDMWYVELAVGGSKVHAGCNGKLVWRHTPWLGAHTAKGPARPLRRALQGLDPRTTASLFADSKCIGEKMINGEECFILKLCADPKTLKARSEGPAEIIRHVLFGYFSQKTGLLVLMEDSHLTRIQSSGGEAVYWETTINSFMDDYRPVEGIMIAHSGHSVVTLFRFGETAMSHTKTRMEEAWTIEEVAFNVPGLSLDCFIPPADLRSGSISEARELPQGGREMSATALAVHRAKVAALENTNDDDMVWKVEV</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
    </comment>
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        <property type="entry name" value="GUARD CELL S-TYPE ANION CHANNEL SLAC1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        </location>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Peptidase M41 domain-containing protein</fullName>
        </recommendedName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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            <begin position="21"/>
            <end position="312"/>
        </location>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
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                <person name="Senalik D."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Macko-Podgorni A."/>
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        </source>
    </evidence>
    <sequence length="524" mass="57350" checksum="A9142D93BAA2FD4C" modified="2024-05-29" version="1">MEAPKVEEISHPPMDQLQGLEYCIDSNPSWGEAIALGFQHYILALGTAVMIPTFLVPLMGGSDNDKVRVIQTLLFVGGINTLIQTLFGTRLPTVIGGSWAFMVPIMSIIHDRSLTAILDPHVRFLSTMRAIQGALIVGSSVQIILGYSQLWAIFSRFFSPLGMVPVVALVGFGLFDRGFPVVGQCVEIGVPMLILFVAFSQYLKHFQTKQVPILERFALLISVALIWAYAHLLTASGAYKHHPEETQMHCRTDKANLISAAPWIKIPYPLQWGAPTFDAGHAFGMMAAVIVSLIESTGAYKAASRLASATPPPAHVLSRGIGWQGIGILLDGLFGTLTGSTVSVENVGLLGSTRVGSRRVIQISAGFMIFFAILGKFGALFASIPFTIFAAIYCVLFGLVASVGLSFLQFTNMNSMRSLFIVGVSFYLGLSIPEYFRQYTAGALHGPSHTNAEWFNDFLNTIFGSSPTVALIVAVFLDNTLEFKESAKDRGMSWWAKFRSFKGDSRNEEFYTLPFNLNRFFPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBN4</accession>
    <name>A0AAF0WBN4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0105019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBN4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="285" mass="30785" checksum="7503F3473BADED16" modified="2024-05-29" version="1">MWIFGLSGKSGFSARSTAEQVTKGVDGTGLVAIVTGATNGIGLETTRVLALRGVHVIMGVRNVKAGEKVKGEILQKHPGSKIDVMEIDLNSQASIRKFAADFIATGLPLNILINNAGIMAPPFTLSKDNIEQQFAVNHLANECGKEGRIVNVASELHRYGYKEGIRFDKINDKASYNPNSAYGQSKLCNILHCVELTRRLKEEGANVTANSLHPGVIATNIAQNSSLLGCIFGCAQIFLKNVPQVNGVSGEYFMDSNKAKASSMARDPELAKKLWDFSLTFTENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WC17</accession>
    <name>A0AAF0WC17_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0205945</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WC17"/>
    <dbReference type="KEGG" id="dcr:108205827"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090404">
        <property type="term" value="C:pollen tube tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010183">
        <property type="term" value="P:pollen tube guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000266">
        <property type="entry name" value="probable serine/threonine-protein kinase RLCKVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF41">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="93"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG86727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="383" mass="43030" checksum="807298322A455F46" modified="2024-05-29" version="1">MGCFPCFGDSNKRSNKKINTTHNQHNDQADDDARHSEIVKTTPKETQKKEESKNAVVVQQLETDQSFGGHGEMEEIKAKPFTFDEMEYATENFKPDHFLGEGGFGKVFKGKLRDTSQVVAIKRLDRNGTQGIREFVVEVMTLGLVDHPNLVKLIGFCAEGDQRLLVYEFMPLGSLEDHLHDPSRKSLDWNTRMKIAAGAARGLEYLHDKMNPPVIYRDLKCSNILLGEDYHPKLSDFGLAKVGPTGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEIITGRKAIDNTKSAKEQNLVAWARPLFKDRKKFHQMVDPLLQGHYPVRGLYQALAISAMCVQEEPSMRPLVADVVTALTYLASQIYDPQIHPVQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WC73</accession>
    <name>A0AAF0WC73_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0104548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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    <dbReference type="AlphaFoldDB" id="A0AAF0WF98"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045:SF20">
        <property type="entry name" value="UDP-RHAMNOSE:RHAMNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042101545" description="UDP-glycosyltransferase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="46238" checksum="B0F73E276D198D12" modified="2024-05-29" version="1">MACVEKLHIVMFPWLAFGHIFPYLKVEASQAHGCKGSQDLFCSIPQGQVPETRVRSSSRTYYSFLGIQHSGLDTRWLCFLLAEAYCFETPHSMVSLQYLHCFLHGIFRPPLIKGDGYRVKPEDFTVKPKWVHFDTNVAMSLYQSLALAPGYETDEETGNVSEAYRVGRGLEYCDMVAIRSSVEFEGDWLKLLQDMYKKPVIPVGLLPDVEDSKEESKEDDNEDWSEIKDWLDKQAKGSVLFVAFGSEAKLTQAQTTELALGLELTGLPFFWAMKKQRGLSDTEPVELPQGFQDRTRGRGMIYTTWVPQTKILNHESVSALLNSSGYSSVHLEEKKLGFQIPRDESDGGFTRESVAESVNLVMLDEEGKIYRDKVKDMQVILSDMDKQNGYVDNLLNYLQNHKFMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFI2</accession>
    <name>A0AAF0WFI2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0208205</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFI2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000467">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF58">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041938103" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="21"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="402"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG88970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="708" mass="77777" checksum="B9557E5610D1EEF7" modified="2024-05-29" version="1">MLALFLFILLVFNSQNPVYCMNDEGYALLSFKQSISEDPEGSLTNWNTSDETPCSWSGVTCKDQQVVSLSIPKKKLKGFLSSSLGSLSQLRHVNLRNNKLFGSLPFQLFRAQGLQSLVLYGNSLSGSIPFDIGKLSYLQTLDLSHNFFNGSLPASLVKCKRLKTLVLSQNNFSGPLPDGFGTSLAALEKLDISINRFSGNVPRDLGNLTKLQGTADLSRNLFSGSIPASLGNLPEKVYIDLTYNNLTGPIPQTGALINRGPTAFIGNPGLCGPPLKNQCDTSASPPSSIPYLPSDFPPQYGGNAAGRGAKRRGLSKGSIIAIVVSDVIGICLVGLLLSYCYSKVCGMRKGDNGYGSDKKWKGKRECLCFRNDESETLSENTEQYDLVPLDTQLGFDLEELLKASAFVLGKSGIGIVYKVVLEDGRTLAVRRLGEGGSQRFKEFQTEVKAIGKLRHSNIVMLRAYYWSVDEKLLIYDYIQNGNLATAIDGQVSYTPLSWSARLKIMKGVAKGLVYLHEYSPKKYVHGDLKPSNILLGQNMEALISDFGLGRLANIAGGTPTLESDRMPFEKIQKKQQDSAPLEVIKVNSPVNIDSCYQAPEALKMIKPSQKWDVYSFGVILLEMITGRPPIVQVDTTTIDIVHWIRLCIEEKTPFLEVLDPYLTQDGDREEEIIAVLKIAMACIQSSPERRPAMRHVSDALERIGVPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WH22</accession>
    <name>A0AAF0WH22_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Zinc finger protein BRUTUS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0208194</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable E3 ubiquitin-protein ligase that may regulate the response to iron deficiency and thus contributes to iron homeostasis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Binds zinc and iron ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WH22"/>
    <dbReference type="KEGG" id="dcr:108209282"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098711">
        <property type="term" value="P:iron ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Zinc finger protein BRUTUS"/>
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        <property type="entry name" value="Zinc finger protein-related isoform 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Hemerythrin-like"/>
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    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
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        <property type="entry name" value="Znf_CHY"/>
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    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0436">Ligase</keyword>
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        <location>
            <begin position="975"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="20">
        <location>
            <begin position="1047"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="1111"/>
            <end position="1153"/>
        </location>
    </feature>
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        <location>
            <begin position="550"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="868"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50089"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG88959.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1227" mass="138606" checksum="32530305FE38CA24" modified="2024-05-29" version="1">MATPLSGIQHGGGVAVMAGATSQLELSNKRSCSKSSASPIRIFLFFHKAIRSELDALHRAAMAFASDVSTDIKPLLERYHFLRSIYKHHCNAEDEVIFPALDIRVKNVARTYSLEHEGESFLFDQLFTLLDPNMHNEESCRRELASCTGALETSISQHMSKEEEQVFPLLTEKFSFEEQASLVWQFLCSIPVNMMAEFLPWLSSSISSDERQDMRKWFRRVIPKEELLQQIIFTWMDGEKINKKRKSCIDHSELKSSSDFEASTSICLVDKGQCPCESSNTGKREILLPDSCPANSTLDRPVDEILHWHKAIKSELNDIAEAARTIQLSGDFSDLSTFNKRLQFIAEVCIFHSIAEDKVIFPAVDAELSFAQEHAEEESEFEKFRCLIESIESAGANSSSAEFYSKLCSHADHIMGTIEKHFHNEEVQVLPLARQHFSPRRQRELLYQSLCVMPLRLIECVLPWLVGSLSEEEARSFLYNMHMAAPASDIALVTLFSGWACKGRPSNVCLSSTATGCCPAKLLTGCKEGSAKPCACTSFMPVQGISLGQKENQESQVKSGNSLQRDEKDASDHPGSTNISKVSFSSQSCCVPGLGVNSNNSLATAKSLRSLAFSPSAPSLNSSLFNWETDVSLTTIGHVTRPIDNIFKFHKAISKDLEFLDVESGKLNDCSEAVLREFNGRFRLLWGLYRAHSNAEDDIVFPALESRETLHNVSHSYTLDHKQEEELFENISSTLAELSELHRNIMTVRSNRHISVSSDHNDNLQRYNELATRVQGMCKSIKVTLDQHILREELELWPLFDRHVSLEEQDKLVGRIIGTTGAEVLQSMLPWVTSALTQEEQNKMMDTWKQATKNTMFSEWLDEWWEGNPSASSQASSSNDANLEGTDIHEAPDQSDNTFKPGWKDIFRMNQNELESEIRKVSRDITLDPRRKDYLIQNLMTSRWIAAQQKLPQARTSEASEGEDLVGCSPSYRDPEREIFGCEHYKRNCKVRAACCGKLYTCRFCHDKVSDHSMDRKATTEMMCMKCLEIQPVGPVCATPSCNGFSMAKYYCSYCKFFDDERTVYHCPFCNLCRLGRGLGVDFFHCMTCNCCLGIKLLDHKCREKSLETNCPICCDFMFTSSESVRALPCGHYMHSACFQAYACTHYICPICSKSMGDMSVYFGMLDALMASEVLPEEYRGRCQDILCNDCDKKGNAAFHWLYHKCGFCGSYNTRVIKVDRNPDCVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WML4</accession>
    <name>A0AAF0WML4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0312012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF187">
        <property type="entry name" value="MUTATOR TRANSPOSASE MUDRA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="165"/>
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        </location>
    </feature>
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        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
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        <location>
            <begin position="332"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="365" mass="41657" checksum="EB1DD9B34D725A6D" modified="2024-05-29" version="1">MVDDPRRLQVGCAKGCPFRMWATYIKSTQSWQVKTLKDEHNCVWNYQNRLVTVKWLADKYGDRIRKNPNWKLVEMQEEFKKELKVDVGKWKCCRVRQAALKGVEEKMVQHYANLRKFAGEILRSNSENTVKIMTSRLQEGDPPRFQRIYICYAGLKKAWKECRPVLGLDGCFLKTVTGGQLLSAVGRDGNHCILPVAIAVVENENYESWKWFLQLLIDDLDLGEGTGKTLISDQQKGLDKAIRELMPYVEHRFCTRHLCANLKKVYPSNLVTNCFWTASTSTHPQAFKKAMKELERVSKGAAEKMNELDPGVWSKALTAINSQKKQVTSQLSTASKSCTGSTTRTTLKGGKKFKPPRKTKDLNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMQ3</accession>
    <name>A0AAF0WMQ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">glucose-6-phosphate 1-epimerase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0311199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMQ3"/>
    <dbReference type="KEGG" id="dcr:108215076"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000022">
        <property type="entry name" value="Glucose-6-phosphate 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="311" mass="35138" checksum="C49319DBC67ED6B9" modified="2024-05-29" version="1">MEKVHVEHCKGTNGLDKVVLREIRGSSAEVYLYGAHVTSWKNDYGEELLFVSNKAIFKPPKAIRGGIPICFPQFSSHGSLDQHGFARNRVWSIDSDPPPFPTSSSSKAFVDLILKPSDDDLKIWPHSFEFRLRITLGPGGDLMLTSRIRNTNTDGKPFTFTFAYHTYFSVSDISEVRVEGLETLDYLDNLQNKERFTEQGDALTFESEVDKIYLSTPTKIAILDHEKKRTFVIRKDGLPDAVVWNPWDKRAKAIVDLGDEEYKHMLCVEAAAVEKPITLKPGEEWRGRQELSAVPSSYCSGQLDPRRVLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WQB8</accession>
    <name>A0AAF0WQB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0311478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WQB8"/>
    <dbReference type="KEGG" id="dcr:108212309"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF18">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="509"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="661" mass="76766" checksum="318394D6802530BA" modified="2024-05-29" version="1">MHFPTLEDAYLYYKEYGRQGGFDVRKSTKKTDRYGNILAKYMQCSRGGNPYANKLRDSSGNYVEGPSRRTSSQRCFCRAQIILKPAAIRGFVIMGFEEEHNHPLATGRNKMFLRCNRNVSVAYQDFIVDFGRANIGPTRAHSLVKEMTGSYDNVGATISDFKNFSRDVKVRIEAKDTDQLLSKFKTRRASPDEGFYYDYKLDKKGRLSGLFWTDVIGRANYDAYGDMISFDPTFRTNRFHMVFVPFTGVDNHWKNVTFAAGLLAKENYKNFKWLLTSFKKAMGRAPTTVITDQCKAIKKAISKWWSSSRHRLCMWHIMAKFPSKIGPKLASNKKFVSKLKDAVYSDHLTPVQFEERWEAVIAEFNLVLNPWLSKMFSICAQWIPAYFSDIEMAGLLRTTSRSESSNFYFQHFHDSGDTLVEFWSSFESAMDRQRMRNADDEKKSQKIPLTDITLANESDAANLYTLELFYLVREEIKSGCYHTIVESMFRDDDCSHFKFKDVLLNDQVFEVSLRHCDNHVICSCKFYFRRGYLCRHSFAALHQCCVKTIPHQFLKARWTKNALQEHSFLGSNHVDAEYDNKERRKLKRTRAWFEFKSLINIAWDNEEKVDEILGTMKKMNNSFSESTRAQTNNVVAHRADRFIPPAEINELRQSDLVASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WS31</accession>
    <name>A0AAF0WS31_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0102586</name>
        <name evidence="4" type="ORF">DCAR_0312788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WS31"/>
    <dbReference type="KEGG" id="dcr:108199245"/>
    <dbReference type="KEGG" id="dcr:108213812"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF70">
        <property type="entry name" value="MAJOR ALLERGEN PRU AR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG93503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="160" mass="17938" checksum="1435E641BCC3229E" modified="2024-05-29" version="1">MGFASFTDEYTSPIAPSRLFKASIVDSHNLIPKLLPQGIKSIEHIQGDGGAGSIKQINFIDGRTFNSVKYHIDELSEDKYTYNYTLLEGDALVENLEKITYEVKFESSPSGGTISKVTSKYYTKGEFMLKEEDIKAGKEKVLSMYKVVEAYLIQNPDAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT10</accession>
    <name>A0AAF0WT10_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0313998</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05529">
        <property type="entry name" value="Bromo_WDR9_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000440">
        <property type="entry name" value="Bromodomain and WD repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000403">
        <property type="entry name" value="PH-interacting protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052060">
        <property type="entry name" value="Bromo_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057451">
        <property type="entry name" value="BRWD/PHIP_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057452">
        <property type="entry name" value="BRWD/PHIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266:SF17">
        <property type="entry name" value="BRWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266">
        <property type="entry name" value="WD REPEAT DOMAIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25437">
        <property type="entry name" value="BRWD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25313">
        <property type="entry name" value="BRWD_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="319"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="578"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1515"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="794"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="995"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1173"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="821"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="864"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="911"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="997"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG94701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1587" mass="178512" checksum="80CA9BEF1D6320E6" modified="2024-05-29" version="1">MDLLKCTSFNENPLSVPLNFPHKAHVKDEEKQEISGHSVEKDLDIDLSEVYFLIMHFLSAGPCQKTIRQLWSELGEHQLLPRRYHAWYSRSDAMSGNRNVDDSSFPLTYEEVVERYSYIEKDHLLKLLKQLMLNATPLRGINDRCDLSAADVPTLLGDGPFSLLGAKCTADMQRTPLPAYLRWPHMQADQVLGLTLREIGGGFRKHHRAPSIRFACYAVAKPKTMVQKMQNIKKLRGHRNAVYCAIFDRTGRYVITGSDDRLVKIWSMETGLSLVSCRGHEGDITDLAVSSNNALVASASNDFIIRVWRLPDGLPISVLRGHTAAVTAIAFSPRPNSVYQLLSSSDDGSCRIWDARSSGGSPRIYLPKRTDGIAGKNNATSSTVPASAASNSHQILCCAYNANGTVFVTGSSDTFARVWSAGKISGDDSEQPYHEIDVLAGHENDVNYVQFSGTAVASRSSILDTSVEENIPKFKNTWYNHENIVTCSRDGSAIIWIPRTRRSQGKVGRWTRAYHLKVPPPPMPPRPPRGGPRQRLLPTPRGVNMIVWSLDNRFVLAAIMDCRICVWNASDGSLVHSLTGHSASTYVLDVHPFNPRIAMSAGYDGKTIVWDIWEGTPVHIYEIGRYKLVDGKFSPDGTSIVLSDDVGQIYLINTGQGESQKDAKYDQFFLGDYLPLIQDEQGNVVDQETQLLTYRRNIQDPLCDSSMVPYPEPYQNIYQRRRLGALGIEWRPSSINYSSGAYIGLGQEYQLLPLADLDMVFEPIPEFLDATLFELENDVINDDTDSEYDIAEEIYSGDEQGNLGNGSSSDSECSEEDNKAARIHGHGLRRSKRKNLQVGSMTSPGRLVKSRKLGDHDDNASSSRRPRKKSRPTRLSKRRKSSTAMSLRPQRIAARSASNVLSEISEASTTGEDEEEWEDVLSESESFLQASDGQSDESDENVRTVHRKYPKVKHGSMDTSGNVIKPPEHVVTQMNGENKKRLVLKISLKDRKKSVPSEITISQGNNQNESSSFFPGPSEETSSFVNDPELSRNHNLNKMRQSEDHEISEDINAVKSCTEYQEALDGRSGSKINGVNRSICMNDDGTSFSGSGQRLAGDSTVCTDPDFVVGTSHSHKLKENPAPKPIKLRIKSKSSLEPSSHFDRSIQMSDAVFSSDRLGNNLEGFNINIDQSTSNNCDGKQDAGAEQCEDVPRVRSKTRSLRLKGPLREKNDISHTFMAGEGNLQAGTPANAKTIFHNPRSDEWMSNLKTIARPRSTRNKRGIDNNNDKHLLAAGNLKTSQRKSNWLLLSEQEGHRYIPQSGDIVVYLRQGHEEYIDESQCSYDKVPWKLFGERIRVVETCLVEGLDYAPVPGSGETCCKVTLKFTDSILLGQTFELTLPDLIDFPDFVVEKTRYDAAIDRNWALRDKCRIWWREDNENGGRWWDGRIVESKDKSVDFPGSPWERFRVRYTIGGTFQQHSPWELHDPDSQWEQPSIDCESKDKMMSYLYKLWKSAEKNQDKYGVAKLNQVTQKSEYMNRFPVPLSIIIIEQRLENNYYRSLRAMEHDVMVMLNNAQLYFGKNAELSRKLSRLSDSFTNTFSKLQQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTW1</accession>
    <name>A0AAF0WTW1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF674 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0415290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTW1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007750">
        <property type="entry name" value="DUF674"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103">
        <property type="entry name" value="OS01G0153900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33103:SF27">
        <property type="entry name" value="OS04G0594700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05056">
        <property type="entry name" value="DUF674"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="52308" checksum="9FE60BA8A3188279" modified="2024-05-29" version="1">MKQVKFQLKLFVEKERNRVVFAEAGSDFIDTFFSFLTLPMGTIVRILNNNLESESRVIGSFSTLYTSVKNLDSGLLWSDECKQMLLSPRNSAEADCLQLKLNLDDSAPGTYFICEDWGCSRNSSAFLSTYCTTRCSCGKLMNRGITVSVTPNVKNVQHKGGVFAREIESYIVSDDLYVFPKQLTVSVELLKKCRVKDLNTESPLTEAFLCDGADRVAIIQSKVGSLADTQTCRDRETTEDAMTIKLIVQKSSNKVLFALAEKNFEEFLFSFLTIPLGGITRLLSGNISVGSVSNLYQSVSSLNSKCNIKSQEVKMLLNFQLAPLHFCKNQIFCLNEAKAPLYYCLSDKRYAESIGCLVEYRVDCNYELKCTPLTLKNPKFEEEYARSNEMFMVTDDLVVSPFSAIASISIMQQLKIYPSDVEEQVVRIGMEEALKILKASFESTAALTNAFSSDLLLKKQLTKEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WW19</accession>
    <name>A0AAF0WW19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
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    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
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        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
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    </dbReference>
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        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 5 OF PYRUVATE DEHYDROGENASE COMPLEX, CHLOROPLASTIC"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
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    </dbReference>
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        <property type="entry name" value="Single hybrid motif"/>
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    </dbReference>
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        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="160"/>
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        </location>
    </feature>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOG96739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="470" mass="49013" checksum="983A178B1902E184" modified="2024-05-29" version="1">MAHLLNTSFISTTKTPSLYRPATLLAQPQLKKPTKIQAKIREIFMPALSSTMTEGKIVSWVKSEGDKLSKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAAVGSAIGLLAETEEEIQMAKDKASTNAGSSSAPVAAAAAAPAPVEAAVSVSAPKANDSAPKAKSVTGSSVHPASEGGKRIVASPYAKKLAKELKVDLSGVVGTGPMGRIVAKDVEAAAATGVVVDTAKTNVSVKPDAGPKVELGSVVPFTAMQAAVSRNMLESLAVPTFRVGYTISTDALDALYKKIKSKGVTMTAMLAKATALALAAHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLPDADKVDIYTLSRKWKELVDKARAKQLQPHEYTTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASQPTIVGTKDGRIGMKNQMQVNVTADHRVIYGADLASFLQTLAKIIEDPKDLTF</sequence>
</entry>
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    </protein>
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    </gene>
    <organism evidence="8 9">
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="32"/>
        </location>
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        <location>
            <begin position="77"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
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        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG96393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="249" mass="28668" checksum="E5C7217D15957351" modified="2024-05-29" version="1">METSALIPPLPDLLLFFSFYLTVYLTAHFLIFKNWSSKLRPEAASCLISYSHGSPAVLLAGKALLSDQNRGFASANTTFQTLVLEYSIAYFLMDLIHYLIFYPTDVLFIAHHLATLFVFMTCRFVVFHGAYAVLMLLIIAEVTSFCQNTWTLACARRKDSEFAHKVFDILSPPFYALYTVVRGFVGPWFLYQMGVFYYSGKGGDLIPTWVWGSWLSVVFMAILVSIVWICNLWVELYRERTRKFETKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X097</accession>
    <name>A0AAF0X097_DAUCS</name>
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        <recommendedName>
            <fullName evidence="11">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
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    </gene>
    <organism evidence="12 13">
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0AAF0X097"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription factor IIIB subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BRF1_TBP-bd_dom"/>
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    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="102"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="501"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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            <begin position="507"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG98108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="544" mass="61283" checksum="10E56F629F08D96D" modified="2024-05-29" version="1">MQWMLSSVFNDDDSNVLRRARNFYRIALERNFTKGRPKHLVEAACLYVACRTSEPDPKPYLLIEFAEFLGRNIRAGCCIFDVVQQLSLQDVQSIQKAVDPSLFIHRFANRLLGQTDVNVETTALKIIASMKRDWMQTGRKPSGLCGAALYISAISHGYSYTKSHIVKVVHICEATLTKRLIEFENTESGSLTIDEFTQKAEKHEAELRSLGQTTTGIKSSDKSEVLCKHKGELTFAHGLCRECYAEFMNFSGGLDGESEPPAFQRAQHERMEKEYAEKNAVDSSCDHMAIQGQNSNYIPGESRDENLHSSEDVNAGGATKEVPIDDASYSKLQGADYISAESPSSETLSDIEDLEVDNYIHTAEETQYKKIIWEELNREYVEEQLAKEAAAAAAKEAYEAHLRNCPEDMEDARKLAAEVAAAMEQSKKERRQKRAAEAKNAAPPRTAAEATRQMLAKKKLDSKINYDVLDELFNDSVSPDNKKSRLNDEVYASGTEYVKQNMKENDSELLPENENEGGPEEFSEGLQYGNEEDYIDDYGYDEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X2J0</accession>
    <name>A0AAF0X2J0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0518536</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG99188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009914">
        <property type="term" value="P:hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF53">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 35-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="772"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1239"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1269"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1325"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1352"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1383"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1414"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1464"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="175"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="906"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="818"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="829"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG99188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1495" mass="169154" checksum="7BE22657342F5D68" modified="2024-05-29" version="1">MDEEKGRQYSERRGSRRMSRSLSRSVSRAAASLGVEEVFGSMRRSSRAADEDEEALRWAALEKLPTYDRLRTTIMKNYNAESRNQGLTPAHKEVDVRKLGVDDKQQFIDKLFKVAEEDNERFLLKLRDRVDKVGITLPTVEVRYEHLTIDADCFVGDRALPTLPNTVRNIFESALSCFGISLAEKTRLTILKDASGIIKPSRMTLLLGPPSSGKTTLLLALAGKLDSSLKVRGEITYNGHKLKEFVPQKTSAYISQNDVHVGEMTVKETLDFSARCQGVGSRYELLTELARREKDAGIFPEAEIDLFMKATSIEGVESSLFTDYTLRILGLDVCRDTIVGDDMIRGISGGQKKRVTTGEMIVGPCKTLFMDEISTGLDSSTTFQIVKCLQQIVHLTEGTLLMSLLQPAPETFELFDDIILLSEGRIVYHGPREHVVEFFENCGFKCPERKGTADFLQEVTSRKDQEQYWVDKSREYRYISVTEFSNRFKRFHVGLRLENELAIPYDKTKSHDAALVFKKYLVPKMDLLKASFDKEWLLIQRNSFVYIFKTVQIIIVAIIAATVFLRTELNTVTENDGGVYVGALLFGMIINMFNGFAELSLTIQRLPVFYKQRDLLFHPPWTFTLPNFLLRVPISLVETTVWMVVTYYTIGFAPEGSRFFKQFLLIFLIQQMAAGIFRLISGVCRTMIIANTGGALVLLLVFLLGGFILPKSEIPNWWEWGYWVSPLTYGYNSLAVNEMYAPRWMNKLGSDNTTRLGLAVLKNLDIYQNQNWVWIGAAALFGFAVLFNILFTFALMYLNPLESKQAIISKETAMEMKASQEESAESQRLKSSSRGAHTLPKSLSASDGNNTREMALQRMTSRGLSRNEDARLEAAAGVSPKRGMVLPFTPLAMSFDKVNYFVDMPQEMKDQGVTDDRLQLLQEVTGAFRPGVLTALMGVSGAGKTTLMDVLSGRKTGGYVEGDIKISGFPKKQETFARISGYCEQNDIHSPQITIQESLIYSAFLRLPKEISKEEKMIFVEQVIDLVELDDLKDAIVGLPGVSGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIHQPSIDIFEAFDELLLMKRGGQVIYSGPLGSNSHKIIEYFEAIPGVPKIKEKYNPATWMLEVSSVAVERRLGMDFAEYYRSSALHQRNQALVKELSIPPAGAKDLYFATEYSQPTWGQFKSCLWKQWVSYWRSPDYNLVRYFFTLAAALMVGTIFWKVGTKRDSSTDLSVIIGAMYAAVLFVGINNCSTVQPIVAVERTVFYRERAAGMYSAIPYALSQVFVEIPYVLVQTIYYTLIVYAMVCFEWTAAKFFWFFFITFFSFLYFTYYGMMTVSITPNQQVAAIFAAAFYALFNLFSGFFIPKPKIPKWWIWYYYLCPVAWTVYGLIVSQYGDVTDTITVPGMSTKPTIKWYINDHYGYESDFMAPVAVVLVGFTVFFAFLYAYCLKTLNFQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X459</accession>
    <name>A0AAF0X459_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0519595</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X459"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16745">
        <property type="entry name" value="RING-HC_AtRMA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="41"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="99"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH00237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="242" mass="27081" checksum="2AA7CA55ECE39F54" modified="2024-05-29" version="1">MAYDQTLQVRSITTADVSLGQHVKPVSDATTSENLNGYFDCNICLDTARDPVVTLCGHLYCWPCIYKWLHVVRSSLESDKQPDCPVCKARISETSLVPLYGRGDTRSDPDAKQPLPRRPPGVRAETMAQEQSQSSPLEPHQQPFHPQHYFSHPLANYPTLSPPNIVTAGMISPTVGMFGEMVYARIFGSSDTSLFSYPHAYPLVHNSSPNPRIRRQELQVDKSLSRVSMFLLCCLILCLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5B8</accession>
    <name>A0AAF0X5B8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xrn1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0520063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5B8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH00689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="13807" checksum="BC5C05B3AD0B1E55" modified="2024-05-29" version="1">MVVVDVIEEEAVEIEGVKIPVDTSKPNPNQLEYDNLYLDMNGIIHPCFHPEDRFSFCCHYYLDVIHLYLCAVLLYYSFYNITACDKVKYEDHILLILIYMCNDDRACKFSNVKWIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X620</accession>
    <name>A0AAF0X620_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0521471</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X620"/>
    <dbReference type="KEGG" id="dcr:108223221"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="531"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="763"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="797" mass="88823" checksum="A2E60458157440ED" modified="2024-05-29" version="1">MITLHSGLFLLPRTPNLSTIARGLSPILRPNSSFFLKPPLFTSPNLKFPMSSSRPSAFDALMSKARAAAKKKPQSEASPAKKRKTQELVSVKEVENASLDDAHQVFDKKSKQENVVKCGDNVGTEVLANGCKEGKVNLSLDEKLAVKKAEAVSLDESIAQLKNKPANFDPKKAAFWGEGKKVPFSFVTRVFDAVDKESGRIVITEIVCNMLRTVIQTSPDDLLPVVYLLANRIAPAHEGLELGIGDASIIKALAEACGCNEAQIRKQYKDLGDLGLVAKASRSSQSLMRKPEALTVTKVFNTFQLIAKESGKDSQEKKKNHIKALLVAATDFEPLYMIRLLQTKLRIGLAEQTLLAALGHAAYYSEKRFKPPANIDSPSEEAAKIVKQVYSVLPVYDKIIPAILTGGVWDLPKACCFTPGIPIGPMLAKPTKGVSEILDKFQDMEFTCEYKYDGERAQIHYLENGSVEIYSRNAERNTGKFPDVVSALSRLKKSSVSSFVLDCELVAYDREKKKILPFQVLSTRARKNVMISDIKVEVCIFAFDILYLNGQTLLQEQLNVRKEHLYKSFEEEPCYFQFATAMTSTDLEEIQKFLEDSVDASCEGLIIKTLSRDATYEPSKRSNNWLKLKKDYMESIGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDSSSEAYQTICKIGTGFTEQVLEERSTSLRSKVIPKAKSYYRYADKISPDVWFETTEVWEVKAADLTISPVYCAAIGIVDSEKGISLRFPRLVRVREDKAPEQASSSEQVAEMYKAQKHTQKHYEDDDKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X640</accession>
    <name>A0AAF0X640_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glucomannan 4-beta-mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glucomannan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0521446</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.</text>
            <dbReference type="EC" id="2.4.1.32"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.32" evidence="11"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X640"/>
    <dbReference type="KEGG" id="dcr:108223759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047259">
        <property type="term" value="F:glucomannan 4-beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051753">
        <property type="term" value="F:mannan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06437">
        <property type="entry name" value="CESA_CaSu_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000015">
        <property type="entry name" value="Glucomannan 4-beta-mannosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF17">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="356"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="190"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH02058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="533" mass="61682" checksum="7D6C2BEB0784DFEB" modified="2024-05-29" version="1">MAEVSANSFMPETFQGTTVDLTSQLGLLWELIRVPLIVPLLKFAVVVCLAMELMLFAERLYMGIVIVLVKLFWQKPEKRYNWEPMKEDLEAGSSTFPLVLIQIPMFNEKEVYKISIGAACNLSWPADRLVIQVLDDSTDPIVKELVEKECKRWAAKGLNITYQIRETRGGYKAGALKEGLKRDYVKDCEYVAIFDADFRPEPDFLRRSIPFLMHNSKIALVQARWRFVNSDECLMTRMQEMSLDYHFTVEQEVGSATHAFFGFNGTGGIWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELPSTFKAFRYQQHRWSCGPANLFRKMVMEIVRNKKVNFWKKVYVIYSFFFVRKIIAHMVTFFFFCVVLPLTIMVPEIDVPKWGAIYIPCIITTLNSVGTPRSIHLLFYWILFENVMSFHRTKATFIGLFEAKRANEWVVTEKLGDGLKNKNSKSNKKFQFNFNIGDRIHVTELGFTAFLFFCGCYDYLYGKNNYFVYMFLQTITFSIVGFGYIGTIVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9L4</accession>
    <name>A0AAF0X9L4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0623398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9L4"/>
    <dbReference type="KEGG" id="dcr:108225855"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041998091" description="Reverse transcriptase zinc-binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="3">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="253"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH03993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43395" checksum="2CAF4282E5736028" modified="2024-05-29" version="1">MAMLNDLLSLLSTISLSKSQDTLIWKPTQGSFSTSEAHKLIRSSSSIHTPTQHWNVIWKLKVLPEIINFIWKLAWGALPTKSILSQRLKTTDSSCQWCTLSPETLSHLFWECQLATWALSFIQSWWNISRNTFYRASNNLFRLLKFFSQKHINRIWKTVVAATLWTIWLARNEAIFNDVRLKQTDIHELIQLRVSIWGMASGIIPFSHIPTWKVNSVGTINVHHHLDVSNYWKIRLENFFAVCMVDAAWLPNCMAKGGIGGVIKNKWGRIIYSFSGPAKAQNILEAEISALLHVFNVIRTSHLHMHKLVVCSDSILAIDSIYEGLEKTFPLLAPNYNINSLLQNSVTINLVPSVINVEAETLAKNGASKTFISCRWALEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9Z4</accession>
    <name>A0AAF0X9Z4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0623393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9Z4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051871">
        <property type="entry name" value="GMC_Oxidoreductase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968">
        <property type="entry name" value="OSJNBA0019K04.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968:SF5">
        <property type="entry name" value="PROTEIN HOTHEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042180859" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="33"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="8">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="6">
        <location>
            <begin position="495"/>
            <end position="496"/>
        </location>
        <ligand>
            <name>FAD</name>
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        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="521" mass="57676" checksum="EF7F495FD94B0EED" modified="2024-05-29" version="1">MKQKLKCMQQFHNLMMIINLFLLLSSFNFCQSKKINTWEEEYPFIKNASSFSSKNREYDYIIVGGGTAGCPLAATLSQNFRVLVLERGGVPFGNKNTSLRNFHINLVDTSPNSPAQPFVVEGVFNVRPRILGGGSSINGGFYSRPSLGSLKELELDPKLAYKSYPWVEKQIVQWPVLNPYFEHLNGTKIGGTLFDKDDIRHSAAQLLKSANPKNLDVLIRATAQKIVFDQSSGKPRAVGVIFKDENGEHHEAVLSKNKRSEIILSSGAIGSPQLLLLSGIGPKAELEKMNISVVHNNQFVGKSMADNPQNNLIIPFNRPVKQSLIQTVGITREGVYIKAISGFGQSPSSIHYTHDNSSLEEQNKELPYEDFNAGGLLFKVSDPLSTGELSLNSTDVDDNPNISFNYFSNPQDLQKCVNGYRILEKLVKTKYLTEFMQPGNNTFQKLLNLTTTETINLIPRTADGKVITSDYEVLGVHGLHVIDGSTFVQAPGTNPQATVMMIGRYMGMKILRKRLGKAAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEM8</accession>
    <name>A0AAF0XEM8_DAUCS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0626121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108227905"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="entry name" value="BNAA03G12380D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34380:SF1">
        <property type="entry name" value="OS01G0221300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="222"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="406"/>
            <end position="434"/>
        </location>
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        </location>
    </feature>
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            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
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            <begin position="500"/>
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        </location>
    </feature>
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            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="707" mass="78611" checksum="92C8C34C7EE26018" modified="2024-05-29" version="1">MDVVESTKSVDLGVDWERQLRECKKKCAGLELVIERKNSEFKCLEDKFRVVVAENLEIKEEFGVLNKKYDDLEKRVLENGNEDRGKEELEEKVLKLMAEKKVFEAEKGKAESEVKVWKAKFEVLELQVMKLEGRNKDCNMAGKVKSETGIRETGSKEVGLVDRLRDGSSHLKCSTHLQAESTVKNSRGEMHNVAGVITSAQPSIEVTNNLKVSEETLATDAPSLAGTGTRTVRGSQVRKQLAYGEGSPRRTRASSIPGGSRRASTGVIDIGDTDGEHEMIIPNASTRENEDNKTVSTDHLGINLNVKECASDNKIEETFLHQSCEDMIGGKENSPLNATRKRRRAANIVNSDSEDEDIQLPNLNVDTQPESVMDFRSNSSPVHGTELGNGVHKIASKRRLVTLGNAASTPKRKGEPEVVTVGEGGGGDDDDDDVPISQLVTNVSSVRAAVLRNRVPYSISKRRLASLRKFSDNWLQKSPNTESNLNALETNYEPRNFKEDDSDDASTDENATESSDESMEGFIDDRSDISVKSDGEDSLLIESDSGNSISKNSDHGEASGDSENISCDDMNYPEIMSQLRRKRDSHTSKWEFEAEMLADFGKDPKMCMKAVCALYRQQTIDEQSIKGAIHRNNRGFSLRDAYRGTSMAEFLTGGSGLELRKSVEELKQHSSKGIELCRELAKHYSKQLFTIYTNKEDPFFHPDKIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XI39</accession>
    <name>A0AAF0XI39_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0727822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XI39"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF306">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH08383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="274" mass="31454" checksum="8FB2D17CBAC4DC5C" modified="2024-05-29" version="1">MTHYDNTIPRTISNKGIEKDAAEQYTRVMFYKIQEEIVGCSGDFTILEWKSVDRLKTIVIKDPDDHLNKFEVSLDLETHDISCSCKLFIRVGYLCRHAFFCLGINGINIIPRQYVINRWLKKAVDRFSTLELGEIPDPVSGDDSRRKKVQECWFLFQSCVSEASNDVGKINLLYEKLKVFSDEIKISLGGSGKRLDQEYVENLMGSKIVQEVTVLPPNQSNNKGSRKRLVNPVEKTLGGKKRIARQCQFCNAMAFHDSRNCPEKKKKEQMEMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIL4</accession>
    <name>A0AAF0XIL4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0727911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIL4"/>
    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH08470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="73" mass="7941" checksum="8A2538070F7FD494" modified="2024-05-29" version="1">MATNPAPEPEMLSAKWSKGNQKEKVNNMLLSEAPKFKLITPSILSDRLGEAFGKAICEAVSLGFIKLLSISHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJX6</accession>
    <name>A0AAF0XJX6_DAUCS</name>
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            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0727793</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108193258"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
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        <property type="entry name" value="Spermi_synthase_euk"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="30"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="186"/>
        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH08354.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="315" mass="34412" checksum="9BFB64638E862B52" modified="2024-05-29" version="1">MAAGEGNGVVPVEMEGANSNNNNNISSVISGWFSEISPMWPGEAHSLKVEEILFQGKSEYQNVMVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVSRHSTVEQIDICEIDKMVVDVSKQFFPEVAIGYEDPRVKLHIGDGVAFLKAVPEGTYDAVIVDSSDPIGPAQELFEKPFFKSVSKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTDGAPVDFKHPVSQIDESARPLKFYNPEIHTAAFCLPTFAKKVIEAKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH10333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="278" mass="31087" checksum="3E7570D03464F8CE" modified="2024-05-29" version="1">MRNTKETEREAEDSDQEINPCTVLLVQEHEEGKPEAIDDLVLHHHQQTFNLESIKSAVVIRQLPSQGLSFQLWPAATAFVSLLDRHDRSSTTLSSLLNRPHLGPLRILELGSGTGLVGIAAAAILGAHVTVTDLSHVIPNLKFNAEANMNAVGLNGGKVEVAALGWGVEKEMEAIKENDEYDVIMGSDVVYHDHLYNPLLQTLKYFLLGDRTKKEMVFVMSHLKRWKKESVFFKKAKKDFDVEVIHKHDPCDGSRVGVLLYTFVAKNKKKKVEVAESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMW4</accession>
    <name>A0AAF0XMW4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0729157</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMW4"/>
    <dbReference type="KEGG" id="dcr:108196653"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000003">
        <property type="entry name" value="Class IV chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF193">
        <property type="entry name" value="ENDOCHITINASE EP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00451">
        <property type="entry name" value="CHITINBINDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 16" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10 15 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042025950" description="chitinase" evidence="17">
        <location>
            <begin position="28"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="18">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH09699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="274" mass="29310" checksum="A3E2EFFA0DC9AA22" modified="2024-05-29" version="1">MLSSTITMNIFATIILAGILLPELTAAQNCGCAADLCCSRYGYCGTSDDYCGAGCQAGPCTAPPPQNGVSVESIVTDSFFNQIIDQAEGSCAGKGFYSRSKFLEALESYSQFGRVGTEDDSKREIAAFFAHATHETGHFCYIEEIDGPSKDYCDENSTQYPCAPNKGYYGRGPIQLSWNFNYGPAGSSIGFDGLNNPEIVAEDPVVSFKTALWYWMNNVHSVITSGQGFGATIRAINGALECDGGNPDTVSARVGYYTDYCSQLGVAPGDNLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNN4</accession>
    <name>A0AAF0XNN4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0729225</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNN4"/>
    <dbReference type="KEGG" id="dcr:108193371"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF58">
        <property type="entry name" value="TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 C-terminal" evidence="8">
        <location>
            <begin position="379"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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    </evidence>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="5">Leaf</tissue>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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            <begin position="20"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOH14289.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1129" mass="126996" checksum="E01390A8DAF1B194" modified="2024-05-29" version="1">MASTSYNQTPSSATSASLPTTWDVFLSFRGIDTRYTFTDHLYNALDRNGIRTFRDDPELRSGEVISDGLLQAIHKSKTYVIVLSENYASSRWCLDELVEILNCYSTTQRSVIPIFYNVDPSAAGHQTGSFREAFEKHETRFGKERLDKWRVALTMLGKLSGKVCKNMYEAQFIEEIVGEILLEINPKTLDVAKYPVGLVPRVKELAALLGNGTEGVIRIGLYGMGGVGKTTLAKAFYNHLLQRSFEGCCFLANIREVSGTRRGLESLQQQLINDVLRSKRQTRVYNVEEGTMLIRERICSAKILVLIDDLDDINQYESLVGPFASGSVVIITTRDEEILEKIDVESRYRYRVNELDDAESLTLFTRHAFENADADNSFKLLSTEILRLAGGLPLALKVFGSHLYKRSKVGWLAYIKKLQKVTDSSIQQRLMISLDALESDDPMLKKLFLDISCLFLGWEIEKVVEILETYYSDADYYIDILRKRCLLYINNRVLGMHDLLRDIGREINGNNSPDEPGKHSRLWVSKDIEDVLKNHEGTEAIEVIFHRNVGKEHAFAAETFRRIRKLRFLQLTGVNLIGGFEGTLEKLRWLCWEYCPLKCFPSEFNPQQLVVLQLPCSSMIQMWKSDNTSRVYDNLKTLNMSNSSYLITTPDFSALPSLETLNFEGCDSLVELDISIGSLGRLVFLKLTGCRKLRSLPDTICNLRALEVLNIGGCFSVEALPEQLGNIESLKELDAHNVALSKLPDSTGRLSKLVKLILTCHRKPMTIYHKDHQKHKTLKTLPDTICNLRELEVLSVGYSRGLAALPVELGNIESLKELDVHDVIVSKIPDSIGCLINLVKLRFTHNKNLETLPKTIGCLRSLKILDISYCRRLIALPVELGNMESLKELYAQSLAVSELPDSIGHLSKLVELRLSDNTKLKTLPDAICNLRSLEILDISYCRSLIALPVELGNMESLKELDAHGLAVSELPNSIGHLSKLVELRLSNNKELTTLPDTICHLRSLEILYIDSCSSLTALPADLGMIDSLKELHARCISVSKIPDSVGRLTKLVKLILRGNKNLKTLPHTMSNMRSLETLDIDDCSDLEALPAELGNIDSLKELNMKNVAVSIVPESIRYLPRAGSRYSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ADK2</accession>
    <name>A0AAF1ADK2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH:quinone oxidoreductase/Mrp antiporter membrane subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0100057</name>
        <name evidence="13" type="ORF">DCAR_0100099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG80954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ADK2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045693">
        <property type="entry name" value="Ndh2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45564:SF1">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45564">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT 2 B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19530">
        <property type="entry name" value="Ndh2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)H-quinone oxidoreductase subunit 2 N-terminal" evidence="11">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="10">
        <location>
            <begin position="146"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG80912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG80954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29636" checksum="7ABDF0C60ED29408" modified="2024-05-29" version="1">MIWHVQNENFILDSTRIFMKAFHLLLFDGSLIVPECILIFGLILLLMIDSTSDQKDIPWLYFISSTSLVMSITALLFRWREEPVISFSGNFQTNNFNEIFQFLILLCSTLCIPLSVEYIECTEMAITEFLLFVLTATLGGMFLCGANDLITIFVAPECFSLCSYLLSGYTKKDVRSNEATMKYLLMGGASSSILVHGFSWLYGSSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFKLSPAPSHQWTPDVYEGVRFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALK6</accession>
    <name>A0AAF1ALK6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALK6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006691">
        <property type="term" value="P:leukotriene metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050997">
        <property type="entry name" value="MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250:SF22">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="120" mass="13898" checksum="3682029DA70928C1" modified="2024-05-29" version="1">MDILIEITMVTYLSPKQLGTRQLYISFLAIGQREFKDKKLINNRRMLGHQNSLEWMPLFLTFMTLGVIKHPDISSVLGVVYIFSRYFYFKGYSTGDPKKRLSVGQLAFSIILINITLICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANZ5</accession>
    <name>A0AAF1ANZ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Chorismate mutase</fullName>
            <ecNumber evidence="3 7">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0206250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="3 7"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANZ5"/>
    <dbReference type="KEGG" id="dcr:108210290"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000001">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF0">
        <property type="entry name" value="CHORISMATE MUTASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="8">
        <location>
            <begin position="197"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="312" mass="35651" checksum="AD11848D51FC7B30" modified="2024-05-29" version="1">MEAKLLRVAPIFPTCLSKPNSNYPRLHQQNYTFQSLNFSHFKKPLQPVQASAASIGVDAKKRIDGSKCYTLDGIRSSLIRQEDSVIFSLVERAQYCYNSDTYDPNAFSEDGCQGSLVEFMLRETEKLHAQAGRYKSPDEHPFFAEHLPEPILPPLQYPQVLHPVSESININSKIWDMYFKKLVPRLVKEGNDGNCGSTAVCDTICLQVLSKRIHYGKFVAEAKFRASPDSYEAAIRGQDKAQLMELLTYPDVEEVIIRRVEMKTRTYAQEVTEDEAEPVYKIKPSLVADLYGTWIMPLTKEVQVEYLLRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AP50</accession>
    <name>A0AAF1AP50_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Histone deacetylase</fullName>
            <ecNumber evidence="2 7">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0206938</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 7"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AP50"/>
    <dbReference type="KEGG" id="dcr:108209017"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000008">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF39">
        <property type="entry name" value="HISTONE DEACETYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="387"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG87707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="49079" checksum="6E849498F5B462A9" modified="2024-05-29" version="1">MRPKDRISYFYDGDAGNVYFGPNHPMKPHRLCMTHHLVLSYDLHKKMEIYRPHKAYPVELAQFHSADYVEFLHRINPDTLNLYPNELAKYNLGEDCPVFENLFEFCQIYAGGTIDAARRLNNQLCDIAINWAGGLHHAKKCEASGFCYINDLVLGILELLKYHARVLYIDIDVHHGDGVEEAFYFTDRVMTVSFHKYGDLFFPGTGEVKEIGEREGKFYAINVPLKDGIDDASFNRLFKTIISKVVETYLPGAIVLQCGADSLAGDRLGCFNLSIDGHAECVRFVKKFNLPLLVTGGGGYTKENVARCWAFETAVLLDTELPNEIPQNEYSEYFAPDYSLKIPGGHIENLNSKSYLGTIKMQVMENLRCIQHAPGVQMQEVPPDFYIPDFDEDEQNPDERMDQQTQDKHIQRDDEYYEGDNDNDHNIEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQN8</accession>
    <name>A0AAF1AQN8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0208625</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQN8"/>
    <dbReference type="KEGG" id="dcr:108208967"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000025">
        <property type="entry name" value="Protein phosphatase 2C 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="14">
        <location>
            <begin position="204"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG89387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="533" mass="58168" checksum="E0A6DB7EC572A612" modified="2024-05-29" version="1">MEDISPAVEATLSLSSLSHDDSEISDNADITHVQMLTDSVSLLLDPVSVLPAESFPGKERSFNTLKKTLSGVVESEKIISVSETVLKGDCGWFPSDTVIQQNKDSFADDGSGIKSKQSLALNANSGISLPIAVKIEEIDDGQIVAKVISLEKQSMEGKMSGTADDASSKVFTLKTSVISLQLPKEMITESGDSRNGVQMDYFPLWGSVSICGKRPEMEDTVAAVPQFLKVPTKMLLGDHLTTEMSQNFHPMTTTHFFGVYDGHGGSQVANYCRDHVHITLKEKLKGVMENETEAGTVDTLQVQWEEIFTSCFQKIDDEVGGKVCHTELGENADTSSGHIEPVAPETVGSTAVVALLCSSHIIVANCGDSRAVLYRGKEARALSIDHKPNREDEYARIEASGGKVIQWNGHRVFGVLAMSRSIGDRYLKPWIIPQPEVMIIPRARDDECLVLASDGLWDVMTNEEACEVARRRILLWHKKNGPITNLNRGQGADPAAHAAAEYLSMLALQKGSKDNISVIVVDLKPQRKFKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASM2</accession>
    <name>A0AAF1ASM2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0313172</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Controls plastid size, shape, and perhaps division during normal plant development by altering ion flux in response to changes in membrane tension. Acts as a component of the chloroplast division machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASM2"/>
    <dbReference type="KEGG" id="dcr:108214808"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1260:FF:000008">
        <property type="entry name" value="Mechanosensitive ion channel protein 3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056876">
        <property type="entry name" value="Msl2-3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057483">
        <property type="entry name" value="MSL2/3_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045042">
        <property type="entry name" value="YnaI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634:SF2">
        <property type="entry name" value="LOW CONDUCTANCE MECHANOSENSITIVE CHANNEL YNAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634">
        <property type="entry name" value="OW CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24956">
        <property type="entry name" value="Msl2-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25237">
        <property type="entry name" value="MSL2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive channel protein 2/3 transmembrane" evidence="18">
        <location>
            <begin position="151"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="16">
        <location>
            <begin position="281"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel protein 2/3 C-terminal" evidence="17">
        <location>
            <begin position="356"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="493"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="720"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="569"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="607"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG93884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="82868" checksum="24E13AA7ECE8E3A0" modified="2024-05-29" version="1">MTTACTLQVSQQLGIYRNHQASRQFQCWPGSGSIHLIGSISSLSLRKDLWSIRLSDRLKRRVPAVSNRCNPFKCHSFLNPGHPFHISNLKNVAVELTRSSGALQGKPVLLKLVPAVTILLFAVWGLGPLMRLSRNIFLHKNDSSWKKSSANYIMTSYLQPILLWTGATLICRALDPVVLPSEASEIVKQRLLNFVRSLSTVLAFAYCISSVIQQTQKYYMETNDPADTRNMGFQFAGKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNIVRNLTQKSHWRVKTHLAISHLDVNKINSIVADMRKVLAKNPQVEQQKLHRRVFLENVNPENQALMILISCFVKTSHFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGDADLDSMPFADSVFTRGRASANRPLLLIEPSYKINGEDKTKPQPRSARANAEQDNKAGARTILDNQLDLKGGPSDVKAKEKQTSDSKEKEASLPDTKEDLTVGDTPYPDSKRDQKSAGIHDSKPGDKVVDKTLSKPGAKVVSKTGEVLSSNSNSPVTDNVTESNSIKKRPKSVGSEGAPQKTVVHDSSSAVIETSGSKANQSTSSSKQTERLLDAQAPASRSVLEENIVLGVALDGSKRTLPIEEGMSSSVAMPEFKELATSRSASMSQTTDENKKNGKNSTVPETAPADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATE7</accession>
    <name>A0AAF1ATE7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATE7"/>
    <dbReference type="KEGG" id="dcr:108213563"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34193:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34193">
        <property type="entry name" value="OS11G0199801 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="209" mass="22580" checksum="DF6DFEF902C07B41" modified="2024-05-29" version="1">MAYYSCVDRSEYGEKGGSDNIEFNIDHGDAESGGGACSSPPLWGDSKMSNNKKMMRSARVEAIARGQKELMEMVKSMPDSSYELSLRDLVDHHQKLGFQQNNNKKKKQQASVRKLQGSSRQTSTSKNYKTGGGLLLKMVFPALPLGATQKSKEIPTYSHTTTAAGGFSSHGSTGSSDTTVTVSSNSTRKKGGLLCGCSPFHSNKKKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B031</accession>
    <name>A0AAF1B031_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0518849</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108221889"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA export from nucleus"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN DELETION OF SUV3 SUPPRESSOR 1(I)-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="9182" checksum="0D13C36AC6519109" modified="2024-05-29" version="1">MAETKIEEKVEEAAKIDLFEDDDEFEEFEIGLEFEEKNEIGTDVTQEWEDDWDDDDVNDDFSVQLRKELQNDTSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1C1</accession>
    <name>A0AAF1B1C1_DAUCS</name>
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            <fullName evidence="5">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0521779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
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        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="451"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02390.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="69219" checksum="17C871A93D53E51B" modified="2024-05-29" version="1">MRVGRKKTAEFSFLLERVGQKLQGWEKQIISRAGKITLLKTAAQAIPNFWMNIMLIPNEICEKIERRMNAFWWRSGNINNKGVHWMSWEKLCVVKEAGGLGFKSLRNFNVAVLAKQAWRLINNSNPLVTKLMKARYYPNSDFLGAKMGSNPSYVWRSILEAQEVIKKGTRRRIGNGDDTMVWKDPWLPCFENGNLTTTMPEELQYTKVKNLMDEHGSKWDEDVLNDLCNDRDKRLIKQIPIPIRSRSDSWYWIQDDKGEVSVKRCYRCIQGEAECTEKAFWRRIWRMELPGKVVNLIWRAARDVLPTTVALQSKHVNVDTRCGWCQINSETAVHVLFECTFAKEIWEMLRLQNVIHTEEGDSVLCVLKRALQSASKEQGLLIGLLRWCLWFRRNKWVWERNNMSTFGIKSLAVNMLTDWRKVLEQGERRTGGVQVQQRQWSKPPAGWLKVNIDASCRQGNEWIGAGCVVRDEEGRFVRARTTVVRGRAYAREAEALSLKEALSWMKTWRHNKCIFESDSKVLIDAIRAGQGKSIFDTHVEDCRELLKHFEDVLVVFVNRSANSVAHELAKAAYSMSGPQEWLYAAPEFIICNLALEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B357</accession>
    <name>A0AAF1B357_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0522293</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEFL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B357"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010851">
        <property type="entry name" value="DEFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33830:SF3">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33830">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 184-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07333">
        <property type="entry name" value="SLR1-BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0295">Fungicide</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041965167" description="Knottin scorpion toxin-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH02903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9042" checksum="625884A45C38A854" modified="2024-05-29" version="1">MAKSLYFYLIVALLYFSAGTMSEVDAQKRCIKTLDPNNCVLSSCKQTCFTQYKGNGVCIAKSGGQSYRCDCVYNCGEELSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B567</accession>
    <name>A0AAF1B567_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0626468</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF1">
        <property type="entry name" value="PURINE PERMEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="150"/>
        </location>
    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="368" mass="40366" checksum="6922DFED15ACDED5" modified="2024-05-29" version="1">MENKEAETDASLHETEVSVLSPSTRKMLLIISCISLSIGNCGGPLIMRLYFIHGGNRIWLSSWLITAGWPLIIIVLIAAFYHRRATTGDYTSKLFNMKLRLILASAVVGGLTGVDNYLFSYGIAKLPVSTSALVIASQLAFTAGFAYLLVKQKFTSFSINAVVLLTIGSGVLALHTSSDRPEGESKKEYILGFVLTLGAAALYGFILPAIELIYQKAKQAVDFPLVMEIQMVMGLFATLFCTVGMLINKDFQAIPREARNFDLGETKYYVVLVCSGIIWQFFFIGAGGVIFCASSLLSGIIIAVLLPVIEVLAVIFFKEKFQAEKGVALVLSLWGFVSYFYGEFKHSKNAEKKAEAEMELQINRTALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
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    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093349">
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    </dbReference>
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    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1551">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="480" mass="53399" checksum="7A58F3D32783632A" modified="2024-05-29" version="1">MESAARSAAKQVHVVCIPSPIQSHIKGMLKMAKLLYSIFITFVNTEFNHRRFLKLGLLESLDGLSGFRFEAIPDGLPPSDPDATQDIPALSHSIIENKMLLPFQNLLAKLNAGIHPVTSILSDGFMPFAADAAQSLGVPVVFLWTVSAGAFMGFCQSRNLLEKGLIPLKDDSCLTNGYLDTIIDWIPGMPNICLRDLPGYVRTTDPDDLILNYIIKATDRARNVTGNVFHTFDDLEPELVNAISTMFPNVCTIGPQQLLLDQEPFEKVEQLKAIGYSLWEEEQTCLQWLDSKEANSVIYVNFGSITVMSPEQLAEFGWGLSNSNHHFLWIIRPDMIVGDSKTTLGLEFMETIKGRGFIAKWCPQDEVIRHPSVGGYLTHGGWNSILESLSAGVPMLCWPHFGDQISNCRYVCDEWECGMEIRNDANRDDVEKLVRLLMDGIEGNKMKSKAMEWKKLAEKACGPDGSSSANLDKLVLLLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B7V4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0625929</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000015">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="14">
        <location>
            <begin position="330"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1288"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1288"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1304"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1348"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH06501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1643" mass="184015" checksum="923B3911FE27C5C0" modified="2024-05-29" version="1">MALSNEVTSESVAAVQFSFMSAEDVRKHSVKQITNPILLDPIAEPIPGGLYDPALGPYDLNKICKTCGQRCNDCPGHCGHIELVSTVFNPLLFDKLYNIIARTCFTCFRFRMDEEEVKLCISKLEMIAKGDIIGARRLDEGGVLDTSTSPEDGETSDFQDPNTWSSLQYTESISVLKKFFKPRPTACKHCKAKNPKITKPTFGWFYMSGLSAADARANIIRASGKTISSVEANSNEDTYVAEIETVESESCMTTSVETEDTRNQRHVQTGKGFSPHSYKQKNTLSRPFLPSEVHATLEQLWANESAILSYICGVQHESPKLTNGQAPSHSMFFIDALLVPPTKFRPPAVTADSVMEHPQTGHIIKVLNANIALCNARANNSEPSIIVRRWLDLQQSINLMFDSKTSMSRSATPGICQLLEKKEGIFRQKMMGKRVNHSCRSVISPDPYLAVNEIGIPPYFALRLTYPVKVTPWNAQKLREAIINGPEIHPGATNFVDKVSNSKLPLKKKGRISVSRKLSSSRGEASTKSHEMEGKIVFCHMKDGDVVLVNRQPTLHKPSIMAHIVRVLPGEKTIRMHYANCSTYNADFDGDEMNVHLPQDEVSRAEAYNIVNANNQYIVPTRGDTVRGLIQDHIGAAVLLTMKDTFLTREQYDQLLYSSGVFSAGPSLSSKSLSKKISKVESYGLQPVLPAVWKPKPLWTGKQTNILTVITSLLNHLTRGCKPCTLENEGKIPYQYFSRNSSNNQLSKDEEASAENKVLIRRNELVRGVIDKAQFGKYGLVHTVQELYGSDTAGILLSALSRLFTCFLQMHGFTCGVDDLILLPQYDVLRKEELEGEDVGEEVHCDFVNFKRGKIGPKELQYEIEKIMRNNGESAVARLDGKMKNELREKGSKINKELLLNGLYKPFPKNCISLMTISGAKGSSVNFQQISSSLGQQELEGKRVPRMTSGKTLPSFSPWDFSSRAGGYITDRFLTGLRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKNLECLKVCYDYTVRDADGSIVQFCYGEDGVDVHQTSFLNKFEALAKNQEIIGQKFHHKLEYNAYIDKLPNKLKERVDELIRTSLKGQFLEQLKQQDLLMLMKQKYISSLAQPGEPVGVIAAQSVGEPSTQMTLNTFHLAGRGEMNVTLGIPRLQEILMTATSEIKTPIVTCRLLEEKSKDDAKALVAKLKKVTVADIIESVEICVVPLYVYKDEICRLYKLKIKIKKHELVSEEDCRETLEITYLRELEDAIENHVQLLARISGIKDFTENIESMSASQTRDAVSGNKSQGNDDDDENGDNEGDDFGFDAQKRKQQASDEIDYEDGIDGKTTEVELSTEVESEIDQDDDEIESSIGHVEDVDVEDEPSNVSYDKGLITEPTSTDRKAQSKSKKKKRGNLARKETDRSCFVKTEGQRYEVHFRFKDEPHILLSQIVQKAAKKVPLIGSGKIEHCQQLAYDVLEKQVLWQKEENGAAEVQKDLSNLCALKVAGVNLTALWEMQDVLDVNHIYSNNIHLMLKTYGVEVARKTIIKEVQDVFKIYGVEIDYRHLSLIADYMTHSGEYRPMSRHGMISESLSPLMKMSFETCSKFIVEAASHGQTDNLETPSSRICLGLPVKLGTGSFDLMQNLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B9C0</accession>
    <name>A0AAF1B9C0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable glutathione S-transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0830524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. HSP26 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B9C0"/>
    <dbReference type="KEGG" id="dcr:108199362"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000012">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF676">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="25111" checksum="42DED84DCB23B435" modified="2024-05-29" version="1">MGDQEVKLFSLWASPFGKRVEIALKIKGIKYEYFEEDLSNKSSELLKYNPVHKKLPVLLHNGKPVCESLVILEYIDETWKSERSLLPEDPYARAMARFWAKFIDDKCIAAMWGARKNSEGQEEGIEELTEHLKTLENELKGKKFFGGDEIGLVDITADLIALWLGVLLELKGIELLTKEKFPRLCEWIDDYLDHQIIKETLPDRETYRDFLLKAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCU4</accession>
    <name>A0AAF1BCU4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0832732</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCU4"/>
    <dbReference type="KEGG" id="dcr:108197421"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="420"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="455"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="525"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="560"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="665"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="700"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="735"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="781" mass="88017" checksum="94649AC8E2DA0549" modified="2024-05-29" version="1">MKCCIEAVCVFAKTMLVFLRTLFSISRKTPRCVSVRILTNSASLSHICMSNSISCGVPDHYSSHYVLPCLVVSVTSYLCLFRQNYVTEVKCDEGLEEGVIFRIVREGRWDDDRIVRLFESGLGSVSVERVYVELKREPGLALKLFKWAERRNGFSHSTESFCILIHILFCSLMYVDAHDLMKELASSNRVVPASDIFDILWSSRNVCTPGFGVFDMLFSVLVELGMFEEATDCFSKMKRFRVLPKARSCNSLLHRLTKSGRENDSRNFFKEMFGAGIAPSVFTYNIMIDYLCKEGDLKSANILLSNMKKLGITPDTFTYNSLIDGHGKIGQLDDSVCLCEEMKEYGVYPDVVTYNILINCFCKYDKLPQAFKFLQEMKENGLEPNVVTYSTFIDAFCKEAMMQEAIKFFIDMRRLGLTPNEYTYTSLVDANFKAGNVGEAVKLVKEMLEAGFSMNIVTYTALLDGFCEEGRMEEAEEVLKSMPEAEVTPNLTIYTALIHGYIKAQRIGDAVNIWQEMKDKNIVPDQMLCGTIVWGLCKEERLEEATLILGEMKECNIQANDVIYTTLIDGYFKAGKTKEALILLEQMQELGIDPTIVTYCVLIDGLCKSGSVDMAIDHFGKMQEMGMIPNVVVYTALIDGLCKAKRVESAKMLFDVMGCKGMLPDKVAYTSLIDGYMKDGNIEEALNFRDLMLEVGMQPDLYTYTTLISGLLKAGHVQQARDLLDEMIMKGITPDEVVYGCLVRKYCDLGDMDEAQKLQTEMVRSNLISGTSHFSVPCVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDS3</accession>
    <name>A0AAF1BDS3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0935558</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDS3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF467">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WSQ8</accession>
    <name>A0A161WSQ8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_010469</name>
        <name evidence="2" type="ORF">DCAR_0311908</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WSQ8"/>
    <dbReference type="STRING" id="79200.A0A161WSQ8"/>
    <dbReference type="Gramene" id="KZN01715">
        <property type="protein sequence ID" value="KZN01715"/>
        <property type="gene ID" value="DCAR_010469"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF134">
        <property type="entry name" value="F14J22.5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN01715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="308" mass="34455" checksum="E2519A014DD54449" modified="2016-07-06" version="1">MPSRAHLFSSSLQQRSRSFDVLECEDQFEDKLPKIGWTDLGEMSEPSYATLTYEFLSSLTVSSNGTLSFKIANVQHEVTEAQLATMFGWELIEQQAPPENYATPFWLKITRLPLKAMFHSLPILQKFLKIVDGWPSELNADYLMGMHMLQRQKSAYVLVTHKRAALNATEEVNEEIAQKPTLEDQVADLQRNGDGGMNAGMCPNRAESLPMNTRTRSLVLVNYFPDTPSSTQACKSNSAPLADMVNTCRVVAGFVEMIAAYQSANDKEWFQVLNQTVTALAQHDMILALLEGMALYSISMERVISISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X6Y1</accession>
    <name>A0A161X6Y1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X6Y1"/>
    <dbReference type="Gramene" id="KZN06685">
        <property type="protein sequence ID" value="KZN06685"/>
        <property type="gene ID" value="DCAR_007522"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210144"/>
    <dbReference type="OMA" id="DTMFAYA"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="723" mass="81434" checksum="D95AE864A6ECD583" modified="2016-07-06" version="1">MLKKIRRRVKIIATKPFKKPRKPPQQQAPSNPPSPPQETTMSPPLHHRKQPNQPFLFPKVQSTILPDPSAFFSPHLLSTPLPTNSFFQNFVLKNGDQPEYIHPYMIKSSLSSVSVCYPSVFSSPAFFYQVFILDLTVSVLNNPDPNSKHVISSFNDLSVTLDLPSSNLRFFLVRGSPFVTFAATNGVEIVISTIHAILECVPNSSGTKYTIKLNNNQTWVLYASSPIKLSNDINKITSSAFSGVIRLAVVPNCQYEEVLDRFSSCYPVSGDALFTKPFSLEYKWEKKGWGDLLMLAHPLHVKLLEMNGSVTVLEDFKYKSVDGDLVGVVGNSWELKTDPVSVTWHSTKGVKEESYDEICTALRKDVEDLNSTTIGTTSSYFYGKLVARAARFALIAEEVCYPDVIPAIKKFLKDTIDPWLNGTFGDNGFLYDGKWGGILTKQGSTDSGADFGFGLYNDHHYHLGYFLYGIAVLAKIDPAWGRKFKPQAYTLMADFMNLGRRENSNYPRLRCFDLWKLHSWAGGLTEFADGRNQESTSEALNGYYSAALMGLAYGDTHLVAIGSTLSAMEIQSAQTWWHVREGDKLYPEEFTRENKVVGVLWSTKRDSGLWFAPPDWKECRLGIQLLPLLPISEVLFSDVEFTKELVGWTLPALAREGVGEGWKGFVYALEGIYDKHAALEKIRNLNGYDDGNSLTNLLWWIHSKDEEEEGFHGRSCWYNHYCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X8K1</accession>
    <name>A0A161X8K1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0729817</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM88883">
        <property type="protein sequence ID" value="KZM88883"/>
        <property type="gene ID" value="DCAR_025958"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195963"/>
    <dbReference type="OMA" id="DSGWHPI"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000009">
        <property type="entry name" value="Auxilin-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF87">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="757"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1083"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1226"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="340"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1126"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1252"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="765"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="790"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1056"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH10350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1391" mass="157177" checksum="21B96604F046A12C" modified="2016-07-06" version="1">METISPPLHKKSYRSGNVAAGKTPYDDVFAARPKVKVPTLSPRDEDYSEIFEVFHASRGSSIPVLDLPAVGEDEDPDVYFDVRSSGFDYSEVFGSSNGFDFALYEQLFQQSKADNDSSNEVWTPAQSESLSDESDPSGCSEKNQSLSSGDIHQSYDDTKQFNISYHKANPKGKDNILDAMTHVTQLDAIPGYSFIISDTSPSKHVEVESLPVQIERNTDCGGAVTEGKRLRKTVSQPPISDIGLQGCKTEASVLAHNDKSRSPGKAFISVSDVNLRTRPSHLPPPSRPPPLFADRAENSSRLNSKFKASKSYVFERTNAGDSQPYFDVEIDASSSAAASAAAVKDAVERAQEQLSNAKEMMERKKDGLENRTKSHFKKDIGQRGEKNIKFDMLKSSKGDIFSPKYAREGSEIKTFAEENEKKARNMIHVASSATEGKEDIHSKQGKEHMQSQVPHETEGTVAWREATQLYEVVEEKKISEACRSADHSTSSDSGVGETGIATAVELQECQMQTKATSADHECQGNVEKFEVFKGGHEQVKIKAAQGSIWQEEHQEKSKLAQMGDECQHMDKETRGALQPGETNDKANAVDKYENDDIGTQDQLEENEVIFIQKIVGANESYEKVIEIMEAPERKGSGMERTASFDSIENTIKFSADVKQERGKFNEATRQSAKDKLEKEVFENHGNESKHVKTYEMGQNKEKQDEVAECKGKTNQLEEVFEQEESNNRLGVDLKWQECKKEQNFVSEAEKIEKGSKITIEQWQNTEREEKASEEAASEERPNDLSEQDETEKRLKEAWEKKEKWKRSREARESEKRVELLFKEDEIMDRSSEANVWIKEGKGEGFIEHDIVQEQLMRVVSDGILNLNQGNYTTMEEPKIFDGGSGHIHEPSVPVENESLEMEHKTHEREVEVNNEPQEANVNSNLDQNGLQYEGINDKNKDEPLCQIGITDMYNEDGIDQTTGIHIKKTSGTNEMASDIENANISTHTRREARRNFKGIQPDQHAFNQAENEDKFTAPRVVKKSVENERKPGNVLPAKKFTSQNPEVEETNFQKKTRLEEREKERLEKERDLENDFLRKLEEEKEREREREKDRMGVDTATREARERAFADTRERAERAAVERATAEVRQRALAEARERLEKASAEARERLIADKASDARLKAERAAVERATAEARERAIEKAKAEKASYESRDRVERPVTDKFSAYSRNGGFRQCSSSNDLQNLQHQGMGPSRDLKHSHSSVSGGLEGESAQRCKARLERYQRTAERAAKALAEKNMRDLIAHREQIERSRLAESLDADVKRWSSGKEGNLRALLSTLQYILGPNSGWQPIPLTDVITAAAVKKAYRKATLCVHPDKLQQRGASIHQKYICEKVFDLLKEAWNKFNSEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XDP2</accession>
    <name>A0A161XDP2_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0624382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XDP2"/>
    <dbReference type="Gramene" id="KZM90813">
        <property type="protein sequence ID" value="KZM90813"/>
        <property type="gene ID" value="DCAR_021822"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="80" mass="9099" checksum="115855E4C91D14DE" modified="2016-07-06" version="1">MKRKITKKLNDLHNDEEIEAALKLVQLRSLKKDIIISCSTSGKSNLCHNDAEYVKEADGPKKKAKKFRSIVDLYNVTKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y7E3</accession>
    <name>A0A161Y7E3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0729160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y7E3"/>
    <dbReference type="Gramene" id="KZM88329">
        <property type="protein sequence ID" value="KZM88329"/>
        <property type="gene ID" value="DCAR_025404"/>
    </dbReference>
    <dbReference type="OMA" id="LCAYFPA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016138">
        <property type="term" value="P:glycoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF76">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 708D1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH09702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="473" mass="52365" checksum="A2847AF0FBFC5E0F" modified="2016-07-06" version="1">MSASRVQNYRLTLRKMANSDNHNHNAHVALIPSSGMGHLTPFLRLAVSLISHNVRVTLITAQTTVSLAESQALSCFFSTYPQITASQLPLVPYDSPSEDPFYIQYEAIRRSSHLLSPILCSITSSLSALITDMSLASTIIPVTQSLNLPNYVLFTSSAQMSTLFLSFHAIAGSRGENDLFTIPGMQPLPISWIPPPLLSEKDSLFRTQLMENGKAMLQADGILINTFENFEQEPLVALNAGEVITRLPPVTAIGPFEPCGFERSRSLTWLDGQPMNSVVFVSFGSRTAISREQNKELADGLLRSECRFIWVVKEKKVDREDGKVLDELVDEEFMKKGKDKGLVVNQWVNQEQILSHPAVGGFVSHCGWNSVTEAAWHGVPILAWPQHGDQKMNAYVLSKRGLGIWAANWGWGGEMVVKGTEISEKIREVMGNEMLRAQVIKIRENARAAVANGGSSHKGVIELIQIWKNKNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y865</accession>
    <name>A0A161Y865_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0207340</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y865"/>
    <dbReference type="Gramene" id="KZN05677">
        <property type="protein sequence ID" value="KZN05677"/>
        <property type="gene ID" value="DCAR_006514"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209365"/>
    <dbReference type="OMA" id="RSMSFKI"/>
    <dbReference type="OrthoDB" id="2014413at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15487">
        <property type="entry name" value="bS6_chloro_cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000006">
        <property type="entry name" value="30S ribosomal protein S6 alpha, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF16">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6C ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG88106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="202" mass="22462" checksum="9F2C19E811551E83" modified="2016-07-06" version="1">MSSFSSPFLKSLTLSSTPSALSFSHTILKSFPVKSHTFLKKNPPGSLSIRAQTLDFSGSFFERGGFGGDDDPPATGGIGLATAVEEKEEPQCPPGLRQYETMAVLRPDMSEDERLTLTQKYEELLVAGGGMYIEVFNRGVIPLAYSIKKKNKAGETNTYLDGIYLLFTYFTKPESMEVLEETLITDDEVIRSSTFKIRKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YEK0</accession>
    <name>A0A161YEK0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0624926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YEK0"/>
    <dbReference type="Gramene" id="KZM91269">
        <property type="protein sequence ID" value="KZM91269"/>
        <property type="gene ID" value="DCAR_021366"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224643"/>
    <dbReference type="OMA" id="KPQHGTR"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd23294">
        <property type="entry name" value="beta-trefoil_MIR_AtSDF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000068">
        <property type="entry name" value="Stromal cell-derived factor 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH05509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="219" mass="24580" checksum="1452C0BD485445D6" modified="2016-07-06" version="1">MAFTCFGVAIFLFLFLDSDFSFNSSPVYASAAASEGVEVTYGSVIKLMHEKTKFRLHSHDVPYGSGSGQQSVTGFPNVDDSNSYWIVRPIPDTNEKQGNTIKSGTIIRLQHMRTRRWLHSHLHASPITGNMEVSCFGDDGNSDTGDYWKLEIEGSGKTWRQDQKIRLFHVDTQGYLHSHDKKFSRTAGGQQEVCGVRLKRPDNIWLASEGVYLPVTESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZP94</accession>
    <name>A0A161ZP94_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_026437</name>
        <name evidence="2" type="ORF">DCAR_0730351</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZP94"/>
    <dbReference type="Gramene" id="KZM89362">
        <property type="protein sequence ID" value="KZM89362"/>
        <property type="gene ID" value="DCAR_026437"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM89362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH10876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="225" mass="24344" checksum="26E94E0CD94DA0F2" modified="2016-07-06" version="1">MYRSLFHGSCSSLRLRSSSPIVKHSNLASSPAIMTTIAATVSTLASSPRFSSLISANQSISQPKFSTASNSPTIISSLSSPAIIKPSLISENSICHSSYSTYAHSSSILSSLSSSPETSIMKPSVVTTRSYGRFPPMQGAIPVKPYDAMNTRTLNAKPSFKVHNLRSSYVNDKGNVVNLIREISKKDPKLYDFLMKTAKELIIVLAELIHLKIAIDTQTDIVSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZS00</accession>
    <name>A0A161ZS00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_026395</name>
        <name evidence="10" type="ORF">DCAR_0730303</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZS00"/>
    <dbReference type="Gramene" id="KZM89320">
        <property type="protein sequence ID" value="KZM89320"/>
        <property type="gene ID" value="DCAR_026395"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193205"/>
    <dbReference type="OMA" id="GPTIMQT"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="26"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="123"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="238"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM89320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="36294" checksum="B0D093C38F4DDD25" modified="2016-07-06" version="1">MSLRTAREESDEDMSLPADIDWHMLDKSKFFFLGAALFSGVSVALYPIVVLKTRQQVSLSQVSSVRNAVSIFRHEGLCGLYRGFGTSLVGTIPARALYMTALEVTKSNVGTATVKLGLSEASASAIANASAGLSAAMAAQLVWTPVDVVSQRLMVQGQQNLKLPSASKYQNGIDAFRKILRADGIRGLYRGFGISILTYAPSNAVWWASYSVTQRLVWGGVSCYSENGVNTLRPDSRTVITVQGVSAAMAGGVSAVITMPFDTIKTRLQVLDGRDDNGRRGPTIGQTFRNLVREGGWLACYKGLGPRAASMSMSATTMITTYELLKRLSAKTPEGSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AMD2</accession>
    <name>A0A162AMD2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_011979</name>
        <name evidence="3" type="ORF">DCAR_0313546</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AMD2"/>
    <dbReference type="STRING" id="79200.A0A162AMD2"/>
    <dbReference type="Gramene" id="KZN03223">
        <property type="protein sequence ID" value="KZN03223"/>
        <property type="gene ID" value="DCAR_011979"/>
    </dbReference>
    <dbReference type="OMA" id="LASNMYD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000002">
        <property type="entry name" value="Alkaline-phosphatase-like family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000033">
        <property type="entry name" value="Alkaline-phosphatase-like family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007832243" evidence="1">
        <location>
            <begin position="27"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN03223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="521" mass="57799" checksum="EE27C503BC8A1F5D" modified="2016-07-06" version="1">MILLDMKQHTVTFAFLSLILMSNVSGQSAATLLKGDEKLGGRKAQAGESITSKLGIEDCTFLYYVDCTQGICYCHLSSVTASEGCEQVSDMKAQSRDTFNPNAFLFLSSSASSLPPSRLLLGRQLNKLPHPVVILISCDGFRFGFQHKTPTPNIKRLISQGTEAETGLIPVFPTLTFPNHYSIITGLYPAYHGIVGNSFIDPKTGDSFDQQNFDSKWWLGEPLWETVVNQGLNAATFFWPGSEVNRSSWTCPSKYCRQFNKSVLFEERVDTILSYLDLPTSDIPSFMTLYLDDPDAQGHKVGPDHPQITEAVANIDRLVGRMISGLEKRGIFEDVHVILVGDHGMVGTCDQRLIFLKELAPWIDIPENWIERYTPLLSIRPPPNVLADDVVAKINKGLNSGKVGNGKYLKVYLKENLPSRLHYSDSDRITPIVGLADEGFKVEMNISVARQCAGAHGYDNAFFSMRSIFIGHGPRFAKGVKVPSFENIQIYNLVTSILNIKGARNNGTSSFPKKVLLPRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ATC5</accession>
    <name>A0A162ATC5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0207275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ATC5"/>
    <dbReference type="Gramene" id="KZN05613">
        <property type="protein sequence ID" value="KZN05613"/>
        <property type="gene ID" value="DCAR_006450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="172" mass="19371" checksum="68D59AFB8872A303" modified="2016-07-06" version="1">METPVIIILAGCRVQMWSNAPNVTHVAPTTFYLNLNHHSVNQLRRMLAIPDFSKKVMAMEKKKKAELLTVEAIKSLDKDSVEEATEEYFPESFKMLATQTFTIKMEIHAANVLKQSNLYWATNICHGFKLEEMDTEMEQQPESQSINTQATTSTAHLSGMSDLNCNSSAVTN</sequence>
</entry>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A164SIR4"/>
    <dbReference type="Gramene" id="KZM86148">
        <property type="protein sequence ID" value="KZM86148"/>
        <property type="gene ID" value="DCAR_023282"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196868"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
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        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
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        <property type="entry name" value="OS09G0416300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPLICING FACTOR, PROLINE- AND GLUTAMINE-RICH-LIKE"/>
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        </location>
    </feature>
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            <begin position="20"/>
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        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM86148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="171" mass="19116" checksum="574917E8C462ACBE" modified="2016-07-06" version="1">MGTEVLRPQDCIREAPAIFTHRKSFHHGVPKVNRKPVRQARPENRRKSPEPKKPATFSEDDLTPSNPKIVLGQVTILRRGESLDLKVKASEKKEISKEMTVYGTGRLGPDPKNVPKQVRFGLPDTYAGSAFFTSPAPSSLPLPSFFRKDCGVKAVDDSVATRDLRRLLRLD</sequence>
</entry>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164T872"/>
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        <property type="gene ID" value="DCAR_024321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="202" mass="24017" checksum="FD45C2645173FCD7" modified="2016-07-06" version="1">MDWDEMYVMINYPEEIPKGRFESFLKVCLELDIEQAHFLYSTKMLLRSESVHHHLNVLKINSTLHPPSHFAFLVFKAMYCPFQWDSTVKEMAAIIIHPYFRKRVPEFIYLIRDIKGDDHYDIFPIYKLCPNAKNKQSFLHTGWFPYERHVWSSSLCNKVVPQSQDVVSPFDQCITINHIFQTQCAYCSMQSILYKVVYGIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="1">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="DCAR_025521"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM88446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="14113" checksum="F30DA594FEFB4265" modified="2016-07-06" version="1">MLLVSFSPLPPSLISYFQNGMVFEVRMGRFKSISSGFGVIRFRVGLSLPGLAAEIFGSVPEADIWFGFGSVRVCKVQEKLIPVRLRVGLRSKAEIFGSVPGRLSIQTSEAEMFGSVSGRFVFAKFRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM90744">
        <property type="protein sequence ID" value="KZM90744"/>
        <property type="gene ID" value="DCAR_021891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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    <feature type="chain" id="PRO_5007853914" evidence="1">
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            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH04885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="92" mass="9520" checksum="4E521F3767FB8D9A" modified="2016-07-06" version="1">MAISKLFIISIILVALTFSSTLTPASATSRNLQAFPTFHFPFFGGSPSGDSSSPPSSGFSFFPPFPFFSSPANGVPGGYMPPATAATTTHTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WNP9</accession>
    <name>A0A164WNP9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_020599</name>
        <name evidence="9" type="ORF">DCAR_0625759</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WNP9"/>
    <dbReference type="STRING" id="79200.A0A164WNP9"/>
    <dbReference type="Gramene" id="KZM92036">
        <property type="protein sequence ID" value="KZM92036"/>
        <property type="gene ID" value="DCAR_020599"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225006"/>
    <dbReference type="OMA" id="VHPHNSN"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="20"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="195"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM92036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH06334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="408" mass="45120" checksum="1BE5914DF8B1B50D" modified="2016-07-06" version="1">MDGIQSKSSFRNFMETAKPYIAMISLQFGYAGMNIITKVSLNRGMSHYVLVVYRHAFATAVIAPFALVLERKIRPKMSFSIFMQIFVLGLLGPVIDQNFYYAGLKFTSPTFSCAMSNMLPAMTFVMAVLCRMEILDMKKIRCQAKVLGTALTVAGAMLMTLYKGKVVEMIWSSHVHPLKSSAPGAPVDSDKDWVKGSILLIIATFAWASFFILQNVTMRRYTAPLSLTSLVCFMGTLQSIAVTFAMEHKPNVWQIGFDMNLLAAAYAGIVSSSIAYYVQGLVMEKRGPVFVTAFSPLMMIIVAIMGSFILAEKIFLGGVLGAVLIVIGLYAVLWGKYKEYKEKEAEEFLEPVKDVNNNNAMMMIKDIEANNEDVMQKNETRIVMSPAAFAISAPIPTLPMLAVEAPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XQV1</accession>
    <name>A0A164XQV1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0519129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XQV1"/>
    <dbReference type="Gramene" id="KZM93487">
        <property type="protein sequence ID" value="KZM93487"/>
        <property type="gene ID" value="DCAR_016732"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG99773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="10853" checksum="09F455E68240A643" modified="2016-07-06" version="1">MKHNTEPDKGTNCTSQTEGDALRRPCVAGLMALQTSQKQLTSLTKDSANALRVHATEAEVTHRLSNAKEAASRPAALESHEDLAKKLHVMFFLVLGIWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YPY8</accession>
    <name>A0A164YPY8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0520686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YPY8"/>
    <dbReference type="Gramene" id="KZM94825">
        <property type="protein sequence ID" value="KZM94825"/>
        <property type="gene ID" value="DCAR_018067"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223878"/>
    <dbReference type="OMA" id="NGNTEHK"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF8">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH01304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="207" mass="22676" checksum="BFD3921C89A2A76D" modified="2016-07-06" version="1">MASSIATLGRPSLSAPPLSLSCFPSNPCPKITYAPPRHTKLITLAIEGDNDFGARDPFPAEIESNFGDKVLGFGSTEHKILIPNLAALSLSRLDCSPKPFALSEQDAQNLLRKVVGWRLSNEGGRVKLQCLWKLKDFKSGVELINRIFNVVEATGHLPNLHLEPPNQVRAELWTPSIGGLSMNDFIVAAKIDEIKTSDLVPRKRAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A171</accession>
    <name>A0A165A171_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_015894</name>
        <name evidence="4" type="ORF">DCAR_0414805</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A171"/>
    <dbReference type="STRING" id="79200.A0A165A171"/>
    <dbReference type="Gramene" id="KZM96744">
        <property type="protein sequence ID" value="KZM96744"/>
        <property type="gene ID" value="DCAR_015894"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216805"/>
    <dbReference type="OMA" id="QAKCPKD"/>
    <dbReference type="OrthoDB" id="696558at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007855102" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM96744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="129" mass="13472" checksum="D304DE05FCAFEB57" modified="2016-07-06" version="1">MVSMRAVNILILLNIIIFTSSVSCNKLPCPPSSSGKSPVPKPEAKCPKDTLKFGVCGDWLGLVHETIGAKPSAECCALIKGLANVEAAVCLCTVIKAHVVELVKLEVPVALSLLLNSCGKKVPDGFVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A9A8</accession>
    <name>A0A165A9A8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_015500</name>
        <name evidence="8" type="ORF">DCAR_0415252</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A9A8"/>
    <dbReference type="STRING" id="79200.A0A165A9A8"/>
    <dbReference type="Gramene" id="KZM97138">
        <property type="protein sequence ID" value="KZM97138"/>
        <property type="gene ID" value="DCAR_015500"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217842"/>
    <dbReference type="OMA" id="DNHMSAT"/>
    <dbReference type="OrthoDB" id="669838at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
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        <property type="entry name" value="HARPIN-INDUCED PROTEIN 1 CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="78"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM97138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG95923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="201" mass="22825" checksum="2BB0692AF78ED95F" modified="2016-07-06" version="1">MANTAPHPQHAAAGPRHSKLLRTIAIVLLALIIIVALAVLIIWLVIKPKRLVYTIEDGSIHGFNLTNDHLDANFYFAIRAYNPNKKVKIYNDKVEVSVYYNDQKVAFNSVSPFFQPHQNVTRLGVSLLARNASLNHEDSKDLRLEKSSGEIEFEVKVKSRIRYRVGKWKSSRRTMKVTCKGVMLHTSSSKGFDRSYCDVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XAL4</accession>
    <name>A0A165XAL4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0416249</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XAL4"/>
    <dbReference type="Gramene" id="KZM97981">
        <property type="protein sequence ID" value="KZM97981"/>
        <property type="gene ID" value="DCAR_014657"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215721"/>
    <dbReference type="OMA" id="GPHRSEG"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16532">
        <property type="entry name" value="RING-HC_RNFT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF0">
        <property type="entry name" value="LP20373P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="449" mass="50712" checksum="8B191253202EE90C" modified="2016-07-06" version="1">MEESSSSSSPSSSQSTNTYTNPNPNPNNSNSSPPPPTQNTNSTRSRRSRYSTLLSTFRFLQAPLSTILSHYFPATALNPNPSHADAAEVSIRIVPGQERNQADHELGLAHSSGSAEIDSLQDDRSYVSGSAASGNGNADSNSVPAGAAAPRTPNQQRYDLQHAARLIEQIIPFSLLLMLVFIRQHLQGFFVTIYITAFLFKSNDILKKQTALKGERKLAVLMGYFLVFTLHVIGIYWWYRNDDISYPLIMVPPKEIPPFWHALFTILVNDTMVRQAAMAFKLILLMYYRNGKGHNFRRQGQLLTLVEYTLLLYRALLPAPVWYRFFLNKDYGSLFSSLTTGLYLTFKLTTVVEKVRSFFSAFKALSRKEMHYGSYATLEQVNDAGNLCAICQEKMHVPVQLCCKHVFCEDCVSEWFERERTCPLCRALVRAADMWSYGDGSTSLFFQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XR74</accession>
    <name>A0A165XR74_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0416737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XR74"/>
    <dbReference type="Gramene" id="KZM98431">
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        <property type="gene ID" value="DCAR_014207"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG97397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="73" mass="7843" checksum="31511CD557153D42" modified="2016-07-06" version="1">MAVVEGSPGDKLVPWSYIGTDSAITLCKATNVCHTYVRDQDPLIISAIEDAEAVWKRGLVEKLGLANGPSRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z240</accession>
    <name>A0A165Z240_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0417973</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z240"/>
    <dbReference type="Gramene" id="KZM99523">
        <property type="protein sequence ID" value="KZM99523"/>
        <property type="gene ID" value="DCAR_013115"/>
    </dbReference>
    <dbReference type="OMA" id="CKCLDQD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000566">
        <property type="entry name" value="Phytosulfokine receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF18">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
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        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="769"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOG98629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1048" mass="115575" checksum="1187F9DA3A631CEF" modified="2016-07-06" version="1">MEVMGFFPLFLVKWLFLVGIVWSSLCPGSASQACHPVDLLALKDFAGNLTNGSIISTWSNDSSCCNWDGVFCRETDNLSDARRVIMLNVSDKGVQGVISHAIGNLEHLKLLDLSHNYFEGELPAEFSKLKQLEFLDLSHNVLTGPAFHALAGLESIRAVNLSSNSFNGSLFSFVDFRNLVALNLSNNFFSGGFNSQVCSSSKKIQFLDLSMNHFTGGLEGLGNCSSSLQQLYMDYNSFSGSLPEALYTMSSLEHFSVSANNLSGPVSQNLSKLSRLETLILNDNQFYGPLPDVFENLTQLKHLVVHSNSFSGLLPSTLTFSSNLRKLDLRNNSLSGTINLDFTRLPNLCELDLGTNHFEGALPESLSSCQKLKSLNLAKNELSGEIPENYANLSSLSILSLSNNSFVNLPKTLSIMQHWKNLTTLILAKNFRGEDLPNNISGFHNLKFFALGNCALKGQVPAWLFNCSKLQILDLSWNHLNGSIPQWIGHMESLFYLDFSNNTLTGEIPKSLAELKSLISANNSILCDQIGIPLYVKRNQSANGLQYKQASSFPPSIILSNNRINGSILPDIGKLKQLHVLDLSRNNISGTIPTSISDMENLEVLDLSSNNLYGSIPSSFNKLTFLSRFSVANNRLRGAIPTGGQFLSFSNSSFDGNPGLCGTLISPCGAASSLEHQPKFSTGSGSKLGRGTILGITVSIGVGVAILLAFVVLRLSKRNMGDPVECFDDDFSRPNRLSGAFGSSKLVFFQNSECQNLTVADLLKSTNNFNQANIIGCGGFGLVYKAELPSGIKAAIKRLSGDCGQVEREFQAEVEALSRAQHKNLVSLRGYCQHGTDRLLIYSYMENGSLDYWLHERIDESSHLTWDTRLKIAQGAARGLAYLHKEPKIVHRDVKTSNILLDERFEAHLADFGLSRLLHPYDTHVTTDLVGTLGYIPPEYSQSLTATFRGDVYSFGVVLLELLTARRPVEVCKGKNCRDLVSWIFQMKSEKRVVEIFDPLIWDKDIEKQLLDVLVIACKCIDHEQRRRPSIDQVVSWLDVVGDEGNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZG40</accession>
    <name>A0A165ZG40_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0309885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_008750"/>
    </dbReference>
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    <dbReference type="OMA" id="LCSCKRP"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044249">
        <property type="entry name" value="XERICO-like"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47258:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE XERICO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG90641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="152" mass="17129" checksum="D4C54A7887AB6D15" modified="2016-07-06" version="1">MGLSPYPSPVDVGVLCIILVSTAKSISTVKEIVWYILHAIGIQVVSWEEYSTESPTNLSGSCESCLETSIEEFRERIPAVKYDSLCSDKQLEHDCSVCLTEFEPEAVINHLSCGHVFHKSCLEKWLKYRNVTCPNCRKHMIPAEDEEDTCPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AUZ9</accession>
    <name>A0A166AUZ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_010715</name>
        <name evidence="9" type="ORF">DCAR_0312178</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AUZ9"/>
    <dbReference type="STRING" id="79200.A0A166AUZ9"/>
    <dbReference type="Gramene" id="KZN01961">
        <property type="protein sequence ID" value="KZN01961"/>
        <property type="gene ID" value="DCAR_010715"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213614"/>
    <dbReference type="OMA" id="NENYEVC"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF162">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    <sequence length="301" mass="33692" checksum="DD0BF61668606B1C" modified="2016-07-06" version="1">MEYENCYRQVERPKYDCLLFDLDDTLYPYSSGLATACSNNIKDYMVEKLGIDRSVIFEMSNLLYKNYGTTMAGLRAIGYDFDYDEYHSYVHGRLPYENLKPDPVLRSLLLSLPIRKVIFTNADKVHAVKALNILGLEDCFEGIICFETLNPTHKNIVSDDEDDIQFTGSRSFSSTTNDNTEIFDIIAHFSQPNASISSLPKTPIVCKPSTHAIEKALEIGKINPQRTLFFEDSVRNIQSGKLVGLDTVLVGTSQRTKGADHALESIHNLRDALPELWVAVNKSSSEVGYSGKVAVEMPVTA</sequence>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Zheng Z."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="8">Leaf</tissue>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CTB3"/>
    <dbReference type="Gramene" id="KZN04306">
        <property type="protein sequence ID" value="KZN04306"/>
        <property type="gene ID" value="DCAR_005143"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208014"/>
    <dbReference type="OMA" id="PWCGTKG"/>
    <dbReference type="OrthoDB" id="773436at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
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        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF137">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 65 ISOFORM X1-RELATED"/>
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            <begin position="75"/>
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            <begin position="1"/>
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            <dbReference type="PROSITE" id="PS50811"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KZN04306.1"/>
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        <source>
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    <sequence length="269" mass="30063" checksum="FA9A0D36EE219A8C" modified="2016-07-06" version="1">MDGRINPFAREQDDYDYSPDYSEDSPPSGLFSDSKMASTSSPRKSRRGLQKRVVSVPIKDVEGSRLKGEISTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCLARKQVERNRADPTMLMVTYSCEHNHPWPASKNNQNRNRNQSENRTQNLDNNPVSTATTTPVSISISSPNVTTSNSDDDQISPNFVMDEEFTNFDSEFGWFTNFETTNSSTLLESPTSARDGIRDADIATIFSTKEGDESFFADLEELPECSLVFGRGVIGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="1">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG88760.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EPF5</accession>
    <name>A0A166EPF5_DAUCS</name>
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        <recommendedName>
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        <name evidence="7" type="primary">LSM1</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation by promoting decapping and leading to accurate 5'-3' mRNA decay. LSM1A and LSM1B are essential for the formation of the cytoplasmic LSM1-LSM7 complex which regulates developmental gene expression by the decapping of specific development-related transcripts. Required for P-body formation during heat stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the snRNP Sm proteins family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG89405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KZN06814"/>
        <property type="gene ID" value="DCAR_007651"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206287"/>
    <dbReference type="OMA" id="LKGTMRR"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000029">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZN06814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="130" mass="14797" checksum="A6814B387B5E20BF" modified="2016-07-06" version="1">MSWAAPPEEDIHLSTSLASYLDKKLLILLRDGRKLLGILRSFDQFANAVLEGACERVIVGEIYCDIPLGLYVIRGENVVLIGELDLEKEEIPQHMTRVPPEEIKRAQKAEREASDLKGSMRQRMEFLDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F7Q3</accession>
    <name>A0A166F7Q3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_008277</name>
        <name evidence="12" type="ORF">DCAR_0209360</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F7Q3"/>
    <dbReference type="STRING" id="79200.A0A166F7Q3"/>
    <dbReference type="Gramene" id="KZN07440">
        <property type="protein sequence ID" value="KZN07440"/>
        <property type="gene ID" value="DCAR_008277"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210001"/>
    <dbReference type="OMA" id="IECHYCR"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000023">
        <property type="entry name" value="AP-2 complex subunit mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="177"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN07440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG90119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="49276" checksum="333EFF21D213918C" modified="2016-07-06" version="1">MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRVHIMQTKELGTCPVRQIGGCSFFYMRISNVYIVIVVSSNANVACAFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDEIMDFGYPQNLSPEILKLYITQEGVRSPFSSKPTDKPVPNATLQVTGAVGWRREGLVYKKNEVFLDIVESVNLLMSSKGSVLRCDVTGKVLMKCFLSGMPDLKLGLNDKIGLEKESQLKSRPAKSGKTIELDDVTFHQCVNLTRFNSEKTVSFVPPDGEFELMKYRITEGVNLPFRVLPTIKELGRTRMEVNVKVKSVFGAKMFALGVVIKIPVPKQTAKTSFQVTSGRAKYNASIDCLVWKIRKFPGQTEPTLSAEVELISTIAEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSGYNTVEWVRYITKAGSYEIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HYC5</accession>
    <name>A0A166HYC5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HYC5"/>
    <dbReference type="Gramene" id="KZN10538">
        <property type="protein sequence ID" value="KZN10538"/>
        <property type="gene ID" value="DCAR_003194"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="110" mass="12403" checksum="16CD6B954BA2B2B9" modified="2016-07-06" version="1">MLVDTHTKVDDHVFSVLLDGREVVSYQKNGEGEVVRMKVFVKRDKLEKVLQAMRANLGQSSVNASILPSSLSSEECINAMKRRRILRGTPVKLSFHYCSSWRPALSSIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IVE3</accession>
    <name>A0A166IVE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0104482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IVE3"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Ellison S."/>
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                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YCT9</accession>
    <name>A0A175YCT9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="15">NAD(P)H-quinone oxidoreductase chain 4</fullName>
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        </recommendedName>
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        </alternativeName>
    </protein>
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        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="17"/>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">NDH-1 shuttles electrons from NAD(P)H, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cellular thylakoid membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069481.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YCT9"/>
    <dbReference type="SMR" id="A0A175YCT9"/>
    <dbReference type="GeneID" id="88614325"/>
    <dbReference type="KEGG" id="dcr:88614325"/>
    <dbReference type="OrthoDB" id="564260at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Plastid Pltd"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00491">
        <property type="entry name" value="NDH1_NuoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022997">
        <property type="entry name" value="NADH_Q_OxRdtase_chain4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507:SF21">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE CHAIN 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 15" id="KW-0521">NADP</keyword>
    <keyword evidence="4 17" id="KW-0934">Plastid</keyword>
    <keyword evidence="8 15" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="6 15" id="KW-0874">Quinone</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="9 15" id="KW-1278">Translocase</keyword>
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    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="191"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="374"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="16">
        <location>
            <begin position="131"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH16765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="500" mass="55981" checksum="1AF61E8D05E672F2" modified="2016-09-07" version="1">MNFFPWLTIIVILPIFAGSIILFLPHRGNRVIRWYTICICILELLLTTYAFCYHFQLDDPLIQLVEDFKWIDFFDFHWRLGIDGLSIGPILLTGFITTLATLAAWPVTRDSRLFHFLMLAMYSGQIGLFASRDLLLFFIMWELELIPVYLLLSMWGGKKRLYSATKFILYTAGGSVFLLMGVLGVGLYGSTEPTLNFATLVNQSYPVALEIILYIGFFIAFAVKSPIIPLHTWLPDTHGEAHYSTCMLLAGILLKMGAYGLIRINMELLSHAHSIFSPWLVIVGTIQIIYAASTSLGQRNLKKRIAYSSVSHMGFILIGIGSINDTGLNGAILQIVSHGFIGAALFFLAGTSYDRIRLVYLDEMGGIAIPMPKIFTMFSSFSMASLALPGMSGFVAEFIVFFGIITSQKYLLISKLGITFVMAIGIILTPIYSLSMLRQMFYGYKLFNAPNSYVFDSGPRELFVSIAIFIPVIGIGMYPDFVLSLSVDKVEVLLSNFVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDV8</accession>
    <name>A0A175YDV8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0933712</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDV8"/>
    <dbReference type="Gramene" id="KZM81665">
        <property type="protein sequence ID" value="KZM81665"/>
        <property type="gene ID" value="DCAR_029278"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202444"/>
    <dbReference type="OMA" id="RWFANED"/>
    <dbReference type="OrthoDB" id="165352at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47468">
        <property type="entry name" value="OS08G0130000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47468:SF1">
        <property type="entry name" value="OS08G0130000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH14195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="446" mass="50593" checksum="770879FC67C1FDC7" modified="2016-09-07" version="1">MAPRGLDDEREGEELDTLNREHLFGVPLIEPHHYRTRFPGAVRLKAYIFDGLGNYYNKEWDLQEGSGQEFCWYHVELPKGNQKLSQSAQYLIDALCPPLKLQDILSLVSTGPFCGHVDGALVFRVNSPGPASSKFTFRIAARVTENSVITVSLGRVPRLGFSPVGQSLLSEVPRVESPSGEHKDRSGTVIEEHVLDFLLRMNHSEEADNPVPKSVSNLVVHIIDTHVDHLQDVVTKFEMDLDSVELDLDRGGFALKKQMLDDRRFPKMHLDLQRLLQVLAHGEQVFPRVKEKCSLKVWFASEDINSLEELTGRFKRLKENVGFIANRVTAVQAGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTGQKDPKLKNGFLNVMLLCVATLVLVLLCFLFSALYTRMDAWRRRKDLTRSLSINRRFSKKSERAGYLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YI09</accession>
    <name>A0A175YI09_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0935706</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ethylene receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YI09"/>
    <dbReference type="Gramene" id="KZM83163">
        <property type="protein sequence ID" value="KZM83163"/>
        <property type="gene ID" value="DCAR_030732"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201651"/>
    <dbReference type="OMA" id="MANHARN"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051740">
        <property type="term" value="F:ethylene binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038199">
        <property type="term" value="F:ethylene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010105">
        <property type="term" value="P:negative regulation of ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16938">
        <property type="entry name" value="HATPase_ETR2_ERS2-EIN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000087">
        <property type="entry name" value="Ethylene receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423:SF633">
        <property type="entry name" value="ETHYLENE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423">
        <property type="entry name" value="TWO-COMPONENT SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH16157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="478" mass="52992" checksum="0AFC4E80E8806047" modified="2016-09-07" version="1">MLRVCDFKSGTPEIIQACYAILVLVLPSGQLRCWGNQEIEIVNAVADQVAVALSHAAVLEESQLVREILAEQNRALQQAKQDAMMANHARNSFQTVMSNGLRRPMHSIMGLLSILQDENLSNAQRTLVHTMATTSDVLSVLINDVMDISPNADARCPFNMKYFSLHSMVKEAACLAKCLCAQKNYLFAIEVEKSLPDYVMGDGRRVFQVILHMVGNLLKDSSVWGCLSIRVFPESGVCLERNDQEWGTRRTNSFDGHVYVKFEIGINNNGAQIESSSDERHWNMGIEEGLSFGVCRKLVQMMQGNIWVVPNPVGFDQSMALVLPFHLRRSIAIGGVETGETSERPDSNSIFRGLQILLADYDHVNLAVTRRLLEKLGCVVSVVSSGYECLGLLGPSPPFHVILLDLHMPDLDGYEIAMRIRKFNPTQPLIIAMSASDTKDVWEKCLQVGFNGLVEKPVLLGGIADELSRVLFEASNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKE3</accession>
    <name>A0A175YKE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_028533</name>
        <name evidence="12" type="ORF">DCAR_0830799</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKE3"/>
    <dbReference type="STRING" id="79200.A0A175YKE3"/>
    <dbReference type="Gramene" id="KZM84045">
        <property type="protein sequence ID" value="KZM84045"/>
        <property type="gene ID" value="DCAR_028533"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199731"/>
    <dbReference type="OMA" id="DEFELWG"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="9">
        <location>
            <begin position="111"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="10">
        <location>
            <begin position="281"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM84045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH11318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="779" mass="88253" checksum="D3E6BD423EF86FC9" modified="2016-09-07" version="1">MATNRASVPRSAAVSKGYNFASSWEQNAPMTEQQQAAIVALSHAVAERPFPAKPDNLSGECNGMSVSTKHSNMEESGDIKAVLVNTNQFYNWFIDLEAAMKSETEEKYQHYVSTLTERIQTCNNILQQVDETLDLFDDLQLQHQAVATKTKTLHDACDRLLIEKQRLIEFAESLRSKLNYFDELENVANSFYSPTMNVANENFLPLLKRLDECISYVESNPQYAESGVYLVKFRQLQSRALSMIRTHISSVLKNTTSQVQAAMLNSGGSKASVSEGVEASIIYVRFKAAASELKPVLEEIESRSQRREYVQILADCHRLYCEHRLSLIKDIVQQRISVFSKKEALPSLTRSGCAYLMQVCQLEHQLFDHFFPSSSEDISSLAPLIDPLCTYLYDTLRPKLIHETNIDTLCELVEILKVEVLGEQLSRRSESLAGLRPTLDRILADVHERLTFRARTHIRDEIANYIPFDEDLDYPAKLEQSAELKSEVISDNRSADVSKFWYPPLEKTVSCLSKLYQCLEQEVFTGLAQEAVEVCSISIQKASKLIMKRSSHMDGQLFLIKHLLILREQIAPFDIEFSVTHKELDFSHLLDHLRRILRGQASLFDWSRSTSLARTLSPRVLESQIDAKKELEKNLKATCEEFIMSVTKLLVDPMLSFVTKVTAVKAALSSGTQNPKLESVMARPLKDHAFAKPATVAEIVQKVSAALQQELPRVMEKMKLYLQNSSTRTILFKPIKTNIVEAHIQVQKLLESEYSSEELKGVIDMVSIHDLQAQLDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YLY3</accession>
    <name>A0A175YLY3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0831607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YLY3"/>
    <dbReference type="Gramene" id="KZM84726">
        <property type="protein sequence ID" value="KZM84726"/>
        <property type="gene ID" value="DCAR_027852"/>
    </dbReference>
    <dbReference type="OMA" id="KVATEMW"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044517">
        <property type="entry name" value="PHOX1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183">
        <property type="entry name" value="PROTEIN CLMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183:SF8">
        <property type="entry name" value="PROTEIN CLMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="392"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="659"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="751"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH12108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="775" mass="85908" checksum="9C222F5F8B187021" modified="2016-09-07" version="1">MGKSGGKKKKGGVSQNPNKVLADSNPLTVSNGGVNLDSPIFLKRAHELKEEGNKKFQAKEYVSALEQYDNALKLIPKTHPDRAVFHSNRAACLMQMKPIDYETVIAECTMALQVQPQFVRALLRRARAYEAIGKYEMALQDVQELLGADPNHGDGLDISRRLKTALGTRLEAQQDLQSRPSPAALGASAVRGAPVAGLGPCLPARPVPKKQAASTAGSVTPTNKPDNKSYSAQPAENGHKSGHDVKFQIPKLVPKASNGSSKSNVNMVNDNQKERTSFSSVSLPSHGQTSKVTLQWRPLKLIYGHDIRLAEMPVNCSFKVLREIVGKRFPPAKSVLMKYKDHDGDLVTITCSAELRLAEVFVDSLMAKEPDTDKSDSFGLLRLHIVEVSPEQEPLIVEEEEEEKQLENEVNKGEYESGSSQSESVVETVETETDGKTEKTTLDEKPSASQDPEGKEVEMDDWLFEFAQLFRTHVGIDPDAHIDLHELGMELCSEALEETVTSEEAQSLFDKAAIKFQEVAALAYFNWGNVHMCAARKKIPIDDSAAKDLVATQLQTAYDWVKEKYSLARQKYEEALSIKPDFYEGLLALGQQQFEMAKLHWSYVLAKKEDLSNWDPTETIDLFDSAQQKMQSATEMWEKLEEQRAKELKDPSSSKIEELLKRKKKQVGGTEGESSQREISPDEAAEQAAVMRSQIHLFWGNMLFERSQVECKLGMTGWDKNLDTAVERFKLAGASEADISTVLKNHCSKADAVEGSEKKEIETSSAKPGNDNHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YP45</accession>
    <name>A0A175YP45_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vesicle transport v-SNARE N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_027441</name>
        <name evidence="13" type="ORF">DCAR_0832094</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Prevacuolar compartment membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VTI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YP45"/>
    <dbReference type="STRING" id="79200.A0A175YP45"/>
    <dbReference type="Gramene" id="KZM85137">
        <property type="protein sequence ID" value="KZM85137"/>
        <property type="gene ID" value="DCAR_027441"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200033"/>
    <dbReference type="OMA" id="YRRVMTN"/>
    <dbReference type="OrthoDB" id="430637at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990019">
        <property type="term" value="P:protein storage vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd15862">
        <property type="entry name" value="SNARE_Vti1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.400:FF:000001">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREs homolog 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000002">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF66">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Vesicle transport v-SNARE N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM85137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH12588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="221" mass="25070" checksum="F7899C12FC1D7636" modified="2016-09-07" version="1">MSEVFEGYERQYCELSANLSRKCNSAALLDGEQKNKQILELQAGLDEADVVIRKMDLEARSLQPSLKAMLLAKLREYKSDLNKLKREVRKLTSAKSDIAAHEELLDSGMVDAHAVSVDQRERLAMSTERLNQSSDRLRESRRAVLETEQLGVSILEDLHQQRETLLHSHTKLHGVDDAIDKSKKILSAMSKRMSRNKWIVGSVIAALVFAILLILYFKLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPQ6</accession>
    <name>A0A175YPQ6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPQ6"/>
    <dbReference type="Gramene" id="KZM85696">
        <property type="protein sequence ID" value="KZM85696"/>
        <property type="gene ID" value="DCAR_026882"/>
    </dbReference>
    <dbReference type="OMA" id="WRARAHI"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538:SF13">
        <property type="entry name" value="OS02G0637200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="69" mass="8612" checksum="663DAC2116C2E57B" modified="2016-09-07" version="1">MEVVIVMYCEPYILVRYGKKKLRRLLWRARAHIERHIKWKKMEKQRISFHYDHFSYSLNFDNGDFGFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQL9</accession>
    <name>A0A175YQL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0832066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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                <person name="Senalik D."/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <location>
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            <end position="23"/>
        </location>
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        <location>
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        <ligand>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="510" mass="58619" checksum="58270F793E0DCE1D" modified="2024-05-29" version="1">MEWNWNYVTCSILISLISVVWYSKRKNSYARSNLPPGPRGWPIFGNFFDLGKLPHRSLEKLRDRYGPVVWLNLGPAKTMVVLSSGAAEELYKNNDLSFADRFQNDAMTSHDYYKSSMAIGLYSSYWRILKRLCTIELFTIKRINEAASIRRKCVDDMVSWIEKEAEKGESGGIEVVNFVFSAIFNIVGNLTVSRDLVDPQSTMSSEFNNSLSSLQECMLRLNISDLLPWLRRFDIQGIRKDMDECLGKAIEIMTGFVKERKEQRRRKQLDVSSEQRKDFLDVLLDYRGTSKDEPVKLTDHQLTIFLMEMFIAGTHTSSATAEWAMSELLINPDKLQKVKDELARVIGGNRKLEESDLDNLPYLQATVEETLRLHPPGPLTLPRRAMHDTKFMGYNIPKGTQVFVNAWAIGRDKENWEDPLSFKPERFLNSSINYKGQSFEFIPFGGGRRICPGLPLAHRMLPLLLGTLLHHFDWKLCDGKPQIDMMETMGLGTRKEKPLMAVPKRRKTLQ</sequence>
</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="3">Leaf</tissue>
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    </dbReference>
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</entry>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
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                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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            <begin position="1"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="76" mass="8362" checksum="2730F53114D881EA" modified="2024-05-29" version="1">MAFTKYIIVFLILLPFIAAIPMPEPPTPSNAVPGNCNRPCGLLYTSGDCNRDCIIKQKARGGACQMYDKILYCCCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6L3</accession>
    <name>A0AAF0W6L3_DAUCS</name>
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            <fullName evidence="1">AT-hook motif nuclear-localized protein</fullName>
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        <name evidence="3" type="ORF">DCAR_0103146</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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    </organism>
    <reference evidence="3" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG83967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="AHL"/>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
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        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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            <begin position="1"/>
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        </location>
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            <begin position="59"/>
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            <begin position="129"/>
            <end position="187"/>
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        <location>
            <begin position="75"/>
            <end position="86"/>
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        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOG83967.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="30085" checksum="7BAD638F6D3E5464" modified="2024-05-29" version="1">MDDNGVIAPDAPPTEPATTQPENSNVVMDDQKIDNGADAVYAPLTHQPEAVASELAEAVPSGVVNNNNDEKNGNESDDVMVTENDDPNAFGNIDSNQFVSPNVVGVTPSAVSNQDTEVVNYQNNGSAAVGEAVKRKRGRPRKYPVTTEGASTVPVASPSLVNSPLQAKSLEKRGRGRPAGSGKKHKAGGLSVTLAVPNGCIFGGGVAGPLIAGSDVQIFVGTFLASEQHLTTPRCNEVLTAPANYISSAEESPPSRALLSEPSGAGGNPYSYSNGVLNNGNQEPVPKVAWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WFG2</accession>
    <name>A0AAF0WFG2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0206290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFG2"/>
    <dbReference type="KEGG" id="dcr:108205810"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG87070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="477" mass="53127" checksum="679BA263B4517BDF" modified="2024-05-29" version="1">MFQPRKPLTIQIPQDVDHDPLPPQQIHIKINKPLISTPHKRSPSTISSPHHHHHHFKNTIISPKSTSLLSSFSLVETLLATKTLALRLATHSCSCCCSHCLLKLPLRISFVLFLLPSLFFFSSNHYFAFLLHFLSVVLFSIVIFILLNVYTPIPLLLARSFPLKLSSLASTPPKRLSSPVVWSIGSKPKSEKRPDTASWVQVHKNGDVYEGEFYKGKCSGSGVYYYNMSGRYEGDWIDDKYDGYGVETWAKGSRYRGQYRQGLRHGFGVYRFYTGDVYAGEWCNGQSHGCGAHTCEDGSRYIGEFKWGVKHGLGSYHFRNGDVYAGEYFADKMHGFGVYRFAHGHRYEGGWHEGRKQGLGLYTFRNGETQSGHWENGVLNISSTAGSPPGSPSTVSDSRVFNAVQEARVVAEKAATAAKVEEKVNIAVAAANKAATAARVAAVRAVQNQMRPQNGNVDITVPVIRGNNDNRKQKYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WG25</accession>
    <name>A0AAF0WG25_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pyruvate phosphate dikinase AMP/ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0207981</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209418"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055495">
        <property type="entry name" value="CWD_DUF7067"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056301">
        <property type="entry name" value="GWD-like_N_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054481">
        <property type="entry name" value="GWD1_pHisD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46999:SF1">
        <property type="entry name" value="ALPHA-GLUCAN WATER DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46999">
        <property type="entry name" value="ALPHA-GLUCAN WATER DIKINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23229">
        <property type="entry name" value="DUF7067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22973">
        <property type="entry name" value="GWD1_pHisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23166">
        <property type="entry name" value="Ig_N_CWD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-glucan water dikinase-like N-terminal Ig-like" evidence="13">
        <location>
            <begin position="85"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7067" evidence="14">
        <location>
            <begin position="212"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glucan water dikinase-like N-terminal Ig-like" evidence="13">
        <location>
            <begin position="339"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glucan water dikinase phosphohistidine-like" evidence="12">
        <location>
            <begin position="922"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG88746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1379" mass="153863" checksum="05EC26007B77C402" modified="2024-05-29" version="1">MSNSVGSNLLHHSLFHNLAIINISPCVSGNYILFPPRIASDLRKPPLFTKFSLTVRRKRSMMSSNYRNAVVTQAVLATDSPFELAGKFNLEGNTELQVNVRAPSPGCLPQIELQVTNSSDNLVFHWGGVRDNNGQWVLPSRHPDGTEVYNNQALRTPFIKSGSTSFLRLEIDDPVIQAIEFLIFDESRNKWFKHKGNNFHIKLPSPNVSISEELVQIQAYLRWERNGKQIYTPEQEKKEFEEARKEILSELEKGNSVDNIRNKITKGKIETKVSKQPEKKKMFTAERIKRKKRDLMQLLNKFTPVPAMETILARPPSLSEMALYAKAKEEQSGGTVLRKNVYKLADKELLVLVTKPSGKTVIDIATDFEDPLTLHWALSENPGEWLAPPSTMLPSGSILLDNAVETQFSSDSSNDKAYKIQSIQIETEENFAGMPFVLLSGENWIKNNGSDFYIDLSSVHKQIQKDASDGKGTAKFLLDKIAEMESEAQKSFMHRFNISADLIEEAKDAGELGLAAILVWMRFMATRQLIWNKNYNVKPREISKAQDRLTGLLQNVYKNHPEYRELLRMIMSTVGRGGEGDVGQRIRDEILVIQSKNDCKGGMMEQWHQKLHNNTSPDDVVICQALLDYIKGDFDVSIYWRTLNENGITKERLLSYDRAIRSEPNFRRDQKDGLLHDLGSYMKTLKAVHSGADLESAISNCMGYTSEGQGFMVGVQINPVSGLPSGIPELFQFILAHVEDKNVEALLEGLLEVREELRPLISNPNDRLKDLLFLDIALDSTVRTAIERGYEELNSAKPEKVMHFITLLLENLALSSDDNENLIYSLKGWNQAISMLSAGDDHWALYAKSVLDRTRLALTSKAELYHRQLQPSAEYLGSRLGVDECAVDIFTEEIIRAGSAASLSVLLNRLDPSLRKIANLGSWQVISPVEAVGVVVVVDELLAVQNKTYEQPTILVARSVKGEEEIPGGTVAVLTPDMPDVLSHVSVRARNSKVCFATCYDPNIFSDLQAKEGNLLHLIPTSADIVYSEAKDIDVTGSSNLDKGSPGGSITLTQKNFGGRHAISSEDFTNELVGAKSRNIGYLKGKVPSWVGIPTSVALPFGVFEKVLSDDLNKGVAEKVHILQNKLEEGEFNVLQEIRRTVLALAAPPQLVQELETKMRNSGMPWPGDEGEQRWDQAWMAIKKVWASKWNERAYFSTKKVKLDHSILCMAVLVQEIINADYAFVIHTTNPSSGDSSEIYTEVVKGLGESLVGAYPGRALSFVCKKDDLNSPKVLGYPSKPIGLFIKRSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDRVVVDYSSDPLIIDSDFQKSILSSIARAGSAIEELYGSPQDIEGVVRDGKIYVVQTRPQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGZ6</accession>
    <name>A0AAF0WGZ6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit (LSU).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGZ6"/>
    <dbReference type="KEGG" id="dcr:108209998"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009955">
        <property type="term" value="P:adaxial/abaxial pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009965">
        <property type="term" value="P:leaf morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF35">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18Y-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="8">
        <location>
            <begin position="235"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="34535" checksum="90461302D671DDEC" modified="2024-05-29" version="1">MVFVKANKPRAYFKRFQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDITAQIISASIAGDMVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKKLEMDEEYEGNVEATGEDYSVEPAESRRPFRALLDVGLLRTTTGNRVFGALKGALDGGLDIPHSEKRFAGFSKDTKQLDAEVHHKYIYGGHVAAYMRTLIEDEPEKYQTHFSLYAKKGIDADNIEELYKKVHAAIRADPTVKKSEKPQPKEHKRYNLKKLTYDERKNKLIERLNALNNAAGADDDDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WIU5</accession>
    <name>A0AAF0WIU5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0208284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WIU5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF83">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00292">
        <property type="entry name" value="POTATOINHBTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7712" checksum="574B10167C48AAF4" modified="2024-05-29" version="1">MESICEGKNSWPELVGEDGLAAAEIVEKENPNVNAIVLLVGTPVTRDFRCDRVWVWVNEDGIVVCTPIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM28</accession>
    <name>A0AAF0WM28_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_0311378</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM28"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="302"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG92119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="625" mass="69453" checksum="1A6348BDDC61EACF" modified="2024-05-29" version="1">MRIERRIYISTFRWLHILFYLWVKISANLEGDALHKLKTSLQDPNNVLQSWDSTLVNPCTWFHVTCNNANSVIRVDLGNAALSGPLVPLGQLKNLQYLELYSNNISGPIPSDLGNLTNLVSLDLYMNSFSGPIPDTLGKLTKLRFLRLNNNSLSGVIPLSLTNIKTLQVLDLSNNHLSGPVPDNGSFSLFTPISFTNNLNLCGPVTGKPCRGSPPFSPPPPLIPTFAVPSPGENGPTGAIAGGVAAGAALLFAAPALAFAWWKRRKPHERFFDVPAEENPEVHLGQLKRYSLRELQVATDGFSNKNILGRGGFGKVYKGRLIDGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCITPTERLLIYPYMANGSVASCLRDRLPSESPLDWPTRKRIALGSARALSYLHDNCDPKIIHRDVKAANILLDEEFEPVVGDFGLAKIMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITRQSAFDLARLANDDDVMLLDWVKSLLTEKKLEMLVDPDLQNSYIESEVEQLIQVALLCTQGFPMDRPNMSEVVKMLEGDGLAEKWEEWQKVEVANQEIELAPHRISEWIPDSTDKLHEVELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN88</accession>
    <name>A0AAF0WN88_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-carbonic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0310308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN88"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF201">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041968328" description="Alpha-carbonic anhydrase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="293" mass="33370" checksum="FD84CA310B26055C" modified="2024-05-29" version="1">MKSILVVLGHIMLIVSLHAATNEAQEFISNGHGEYDYDEDSGRGPSEWGNLKPEWALCKKGKMQSPVDLTNVTVKLVSDSDVVYAEYQPSFTTLLNRGHDVALEWKGDAGSIEINGVEYRLQQVHWHAPSEHTIRGKRYELERHAVHVNSDTNEIAVISVLYKIGRNDPFLFKLRRYLKTMVETNVNETFPGIINPSDTTCHDDESFYRYTGSLTTPPCSEGVIWTVQRKIRTVSRRQVDLLLNVVHGNENARPLQAVNKREMFLYVSCKGTGWDLILNPLQYIQRKLFYSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVJ9</accession>
    <name>A0AAF0WVJ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific lipid-transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0414988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG95661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="16177" checksum="3701B3254525A6EF" modified="2024-05-29" version="1">MKSLTLLLVLIFLLSPTSEAAITCSDVIGNLRPCISYLTSGSGKPPSPCCAGVSTLSTSATTTADKQTACNCIKNASKNLNVNTALAQALPSNCGVSLPFTVSPTVDCSKYFFSTSLSPQLYNIMITFNCWLKCTGLKFGSFFFHLFYRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0415205</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>eudicotyledons</taxon>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16472" checksum="9411D44E76B0DAF4" modified="2024-05-29" version="1">MITGLRFRGYRIQSDSDFTIERALDLLGKPGAKRDGKINLNSIKPKPEEVSADPATDDEKEKIFRRLFLYVVGSCFFNNNRSVISHHFVKFLERINKVGSYDWGSITFATFLAGMRRKVTGEIGALTGFWQFLPVHFLSALYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WVY3</accession>
    <name>A0AAF0WVY3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0415840</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WVY3"/>
    <dbReference type="KEGG" id="dcr:108215728"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048829">
        <property type="term" value="P:root cap development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000002">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF39">
        <property type="entry name" value="AUXIN RESPONSE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="12">
        <location>
            <begin position="121"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="13">
        <location>
            <begin position="612"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG96504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="698" mass="77073" checksum="3B6F665C7A51283C" modified="2024-05-29" version="1">MITFMDSKEKFKDTEKCLDSQFWHACAGSMVQMPPVNSKVFYFPQGHSEHACQNVDFMNCPRLPPYVACRVSSIRFMADPDTDEVYANIRLVPISGNEINCDDAGIPGNNGSGEQDKPTSFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSADPPVQTILAKDVHGETWKFRHIYRGTPRRHLLTTGWSTFVNQKKLVAGDSIVFLRAENGDLCVGIRRAKRGIGGNPDSTSGWNPAGGNSVVPYGGFSAFLRDDENKLARYGNGGSPPGCGNGTSKAKVKAESVIEAATLAAEGQPFEVVYYPRASTPEFCVKASLVKSAMQIRWCSGMRFKMPFETEDSSRISWFMGTISSVQVADPIRWPDSPWRLLQVTWDEPDLLQNVKSVSPWLVELVSNMPSIHISPFSPPRKKLRLPQHPDFPLDGQLPVPTFSRNLLGHNSPFARFPDNTPAGMQGARHTQYGLPLADFHLQKLQSGLFPPGFSPFRHCSIPARPSINPDINMSSSSENISCLLSIGNSAHNEKKIEDQKPRQFLLFGQPILTKQQTMLSSSSFTSSGGDIDKVARMSAGDSYASGSGFALRQCTIREPSSSEGLLQCKGNLQEAETSLEIGHCKVFMESEDVGRTLDLSLLSSYEELYRKLATMFSIKSSDMLKHVLYRDIRGAVKHIGDEQFSDFVKTARRLTILTDASSNDIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WWA0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0416035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WWA0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF78">
        <property type="entry name" value="OS12G0587133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG96699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23704" checksum="03E95F898E228275" modified="2024-05-29" version="1">MQDISHLQFADDVILFVNNDDCSVLGVKRTLQCFQVLSGMKINFDKSHIFGFHASTAQINRWASTLGCRHGSLPLSYLGASIGSSPSAIKFWDPLINCIRKKLSEYDTSHISMAGRIILLKSVIDSTPIYWLSLYKLPVTTTRFWQIRREIFDFVCAVDAAWKLNKGGIGGFIRNRTGKVIYNFSGPSTSATCVLEAEIEAIVHRHGSSPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZI9</accession>
    <name>A0AAF0WZI9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0416157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZI9"/>
    <dbReference type="KEGG" id="dcr:108218478"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF6">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
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        <location>
            <begin position="368"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="474"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="510"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="580"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="615"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="77026" checksum="2E23AED7CE96BD06" modified="2024-05-29" version="1">MFLTKPQFLKTSTILTSKHLPHTHSLFSTHSSPLQEHHHLTDTTNTNWTKNIHDLCTTHRDVDSALRLLDHLRLHNYHPNSLNLSSIIHALCDAHRFSEAHQRFLMFISCAHTVPDERTCNVIIARLLSYRNPEATLVVFNRLGEIKPHFVASLCNYNRLIDQFCGVLRVRDAHRLVFEMIRRGHSPNVVSYTSLINGYCKVGEIGDAFKVFDEMCENDVRPNSLTYSVLIRGVFRKRDVDQGRVLMGKLWGVMGDEDEVSVNSLAFGNLVECLCSEGLFSEVFEIAETMPQGKNVCDDFAYGEMINTLCKYGKYHGASRITYIMRKRGFVPSFVSYNAIIHGLSKTGGCMRAYQLLEEGNGVGYVPSEYTYKVLLESLCRESDIGKARNVLEIMLKKEGVDVTRIYNIYLRALCLVNNPSELLNVLVSMLQTECKPDVITLNTVIHGFCKMEQIEEPLQVLSDMMAGKFCSPDAVTFTTIICGLLRLGRTEEALDILHNVMPENGFVPGVVTYNAVLRGLFKLDRTKEAMDLFNSMSTGSVAADSITWTIVIEGLFKSNQVDEAKSFWDDVVWPSKIHDNFVYSAILKGLCNAGKFDEACDFLYELVDAGVNPNAVSYNILMDAACKLGMKKEAYRIVGEMKKNGLTPDAVSWRILDKLHGNVRSRSFVEDSGLQDKIRIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0E7</accession>
    <name>A0AAF0X0E7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HEAT INTOLERANT 4-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0416685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X0E7"/>
    <dbReference type="KEGG" id="dcr:108215970"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900034">
        <property type="term" value="P:regulation of cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039313">
        <property type="entry name" value="HIT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33704">
        <property type="entry name" value="PROTEIN HEAT INTOLERANT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33704:SF1">
        <property type="entry name" value="PROTEIN HEAT INTOLERANT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG97345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="39159" checksum="4864D330822260FF" modified="2024-05-29" version="1">MRKGAKRKATQKDQAVEKKEVVKAKRTRVPKPVSPPDYFPEKRNLEDLWQQVFPVGTEWDQLDLVYQYKWNFSNLENAFEEGGELFGKKVYLFGCTEPQLVFFEGQGKVTVIPVVVAVDSPFPPSDKIGINSVQREVEEIIPMKQMKMDWVPYIPLENRDSQVERLKSQIYILSCTQRRAGLKQMKLDRVKKFEYCLPYFYQPFKEDELEQSTIVPIIFPAEPKPVFTEFDWEFDEVEEFTDKLIEEEELTADQKDAFKDFLKEKVREAKKANREAREARKKAIEEMSEETKTAFEKMKFYKFYPVSSSDTPDVSDVKSPFINRYYGKAHIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X2L5_DAUCS</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X2L5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37718">
        <property type="entry name" value="BNAC03G61340D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37718:SF2">
        <property type="entry name" value="OS03G0205150 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG98575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="83" mass="9659" checksum="32E7D0C837D00423" modified="2024-05-29" version="1">MDPRYIQETSKHLEKQHELLWDTYRSMTHELQKLQVEEEMLMRKFYEAMSAQGLNKKASMSDNLSASPPTPTTSSSDLPATRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X387</accession>
    <name>A0AAF0X387_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MalT-like TPR region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0519081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108224134"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="234"/>
        </location>
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        <location>
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            <end position="551"/>
        </location>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="62421" checksum="25ADB8D16158C391" modified="2024-05-29" version="1">MSIRRGVGGLAKRLETCYPFLNSYLRPTTAVTTTTTTHLPGEHSISPTYFDDSRSAFSWIFLAAPSVLFLGVNSTTVLAEDVSVGLKSEDEISSETISGLRKIEDGSVVSNIHTSKWRVFTDTGRDRFMQGKMEDAERLFQAAIQEAKEGFGERDPHVASACNNLAELYRVQKVFDKAEPLYLEAVKVLEESLGLEDIRVGAALHNLGQFYLVQQKLDKACVSYERALKIKRRVLGEGHTDYADTMYHLGTVLHLLGKGKDSEALIQDSIRILEDNGQGESVICIRRLRYLAQIYIKSSRAEAAMNIQRKILQKMELTKGWNSLETVIAAEGLALTLQSVGSSREAQELFERCLHARKMLLSKDHIQIAGNLLHIARVVMHNSSQLKKTCSSEAITEFNKAKDLLHESVRISLCALDKLMEEKKKKLKGISRETIRNGHAALIILLQSYNTLGTLEISKMGISGHERNYGLVLAAKKSFDQCISAFKEYGTWRELSNSSEAKAEYLSCLKHLSALLSEHEKDMRLSTRTTLQELEDEIKIVEGELQFDKRKTGSWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3B1</accession>
    <name>A0AAF0X3B1_DAUCS</name>
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        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0519127</name>
    </gene>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3B1"/>
    <dbReference type="KEGG" id="dcr:108219963"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000066">
        <property type="entry name" value="Extended synaptotagmin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000102">
        <property type="entry name" value="Synaptotagmin-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF214">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="15">
        <location>
            <begin position="67"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="240"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="401"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG99771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="61050" checksum="11165A8B8821E23D" modified="2024-05-29" version="1">MGIVSTITGFIGFGMGISLGLLVGYYLFIYFQSTHVKDPVIRPLVEHDAETLQSLLQEIPVWVKNPDHDRIDWLNKFIEKMWPYLDKAICKQVKTIVDPIIAEQIPQYKIDSVQFEVLSLGCLPPAFTGMKVYSTEEKELIMEPTLKWAGNPNVLVVAKAFGLKATVQVVDLQVFASPRITLKPLVPSFPCFAKILVSLMEKPHVDFGLKLLGADAMSIPGLYSFVQEIIKDQVANMYLWPKVLEVQIMDPAKAMQRPVGILNVKVVRAMKLKKKDLLGASDPYVKLKLTEDKLPSKKTTVKQKNLNPEWNEEFNLVVKDPESQALELVVYDWEQVGKHEKMGMNVVHVKDLTPEETKALTLDLLKNLDPNDSQNEKSRGQLVVEAMYRPFSDDEKPDDAEDSEVQKAPEGTPDGGGLLVVIVHEGQDLEGKHHTNPSVRLLFRGEERRTKVVKKNRDPRWDEEFQFMLEEPPTNDRIHVEVVSTSKRMGLLYPKDTLGYVDISLADVISNKRINERYHLIDSKNGTVQIELQWRTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X5K1</accession>
    <name>A0AAF0X5K1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroflavonol 4-reductase</fullName>
            <ecNumber evidence="8">1.1.1.219</ecNumber>
            <ecNumber evidence="7">1.1.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydrokaempferol 4-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0520103</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="8"/>
    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5K1"/>
    <dbReference type="KEGG" id="dcr:108222766"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
        <property type="term" value="F:flavanone 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009718">
        <property type="term" value="P:anthocyanin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF713">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="14">
        <location>
            <begin position="7"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH00729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="333" mass="37302" checksum="D7CDF479CD533FB9" modified="2024-05-29" version="1">MEEKRTVCVTGASGYIGSWLVKTLLERGYHVRATVRDPGNEKKVKTLLELPKASTHLSLWKADLAEENSYDEAIQGCKGVFHVATPMELVHQGQDGEEEMESTTLNGILSIMRSCSKANTVKRFVYTSSTGTITVQAQPPLHEYTEDLWTDVDLCYELKMYGWMYLVAKTTAEKAAWKYAEENGINMVTIHPSIVLGHFITPHTSFSTDVATAFYTKNEANMALLKKLNGSNAVHLDDVCNAHIFLFEHPLAKGRYICSSHTVNLFEIAHSLSLKYPDRNILTEFEDLDRSPKIIPCSSKKLMALGFEFAHKNKGVCDFCAETIEACKEMGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X681</accession>
    <name>A0AAF0X681_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NIF system FeS cluster assembly NifU C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0520598</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X681"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000003">
        <property type="entry name" value="NifU-like protein 3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000006">
        <property type="entry name" value="NifU-like protein 3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF25">
        <property type="entry name" value="NIFU-LIKE PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU C-terminal" evidence="8">
        <location>
            <begin position="57"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="NIF system FeS cluster assembly NifU C-terminal" evidence="8">
        <location>
            <begin position="138"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH01217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="203" mass="22349" checksum="9C3EA33BAAABF24F" modified="2024-05-29" version="1">MIQLSNQAQQHIHSSSSYISLHKRFSWLKWKTRTHLNGLLRNVLSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVVLKLQGACGSCPSSTMTLKMGIETRLRDKIPEIMDVEQILDTETGLELNEENVEKILGEIRPYLTGTGGGVLEFVQIDDFVVIVRLSGPAAGVMTVRVALTQKLREKIPSIAAVQLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAI0</accession>
    <name>A0AAF0XAI0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0622921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAI0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052929">
        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074">
        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF11">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="121"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="252" mass="28488" checksum="EABD1501A7B07CAA" modified="2024-05-29" version="1">MAACFSLTSILRFYASPVRAKIWRVVVVATLWSIWLARNELIFNGTRLKKQQLIELIAVRINKWGNAAGLILFGDDPLWRVNPHGIIAVHHFKRTLGFWQFRVDNYDFVCAVDGAFGSQGEVSPKGGIGGYILNNRKEKILIFSGPVRAKSALQVETEGILQMLRWIFSLGLSKNRVLICTDSTESINIFNKGYAKVFPLRECNLDFQHLIGFNIFIQFVPSEINEEADLLAKNGIGRKEMSSWRGLLDGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAL9</accession>
    <name>A0AAF0XAL9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">15-cis-phytoene synthase</fullName>
            <ecNumber evidence="4">2.5.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0622981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34475"/>
            <dbReference type="ChEBI" id="CHEBI:27787"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="4"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAL9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046905">
        <property type="term" value="F:15-cis-phytoene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000004">
        <property type="entry name" value="Phytoene synthase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH03582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="386" mass="43959" checksum="F7611DD94693E5AE" modified="2024-05-29" version="1">MACNFAVRVIYYPKEIHGVSVLNTNRSRKSRFSCRVMKLSTGVSAVAANPVRTSEERVYEVVLKQAALVREEKRSSRGLCLDTKRTGSKSFDKSENDDAGMKSWNLLNEAYDRCGEVCAEYAKTFYLGTLLMTPERRRAVWAIYVWCRRTDELVDGPNASHITPKALDRWEKRLNDLFDGQPYDMYDAALADTVSTYPVDIQPFKDMIDGMRMDLKKSRYQTFDELYLYCYYVAGTVGLMSVPVMGIAPESKATTESVYSAALALGIANQLTNILRDVGEDARRGRIYLPQEELKLAGITPEYIFKGKVTDKWRSFMKGQIKRARMFFDEAEKVWASLLLYKQILDAIEANDYDNFTKRAYVGKAKKLVSLPLAYSRALFAPSTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XCE4</accession>
    <name>A0AAF0XCE4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0624644</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XCE4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="425"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH05230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="600" mass="67559" checksum="D3DD4F70DD9CF65C" modified="2024-05-29" version="1">MANESTSDHDPDDSEADFVEVDPSGRYGRYKEVLGKGAFKKVYKAFDELEGIEVAWNQIKVADLLRNSEDLERLYSEVHLLKTLKHKNIIKFYNSWVDTKTENINFITEIFTSGTLRQYRKKHKHVDLRALKKWSRQILEGLSYLHSHDPPVIHRDLKCDNILVNGNQGEVKIGDLGLAAILQQARSAHSVIGTPEFMAPELYEEEYDELVDIYAFGMCLLELVTFEYPYVECANAAQIYKKVTSGIKPASLIKVKDPGVKIFIEKCIAKVSDRLSAKELLMDPFLRSDENNEATGQSSQSCRQQAGKYFWSLSLHRFHSFSYSCHIRNIHFPFDIEVDTSIAVASEMVEELDLTDQDVTAIAEMIDTEIHSYIPEWAPGDLFGDHTDTEVGICENFVSEGQDATSPLTNESGARSGSLVLERMPSGRKYWSDSPKTGDACSPHRLGPSNLSHEESATPGGSLTDEDVQSTASCQYEDNVNDDASPEKQNVGVLDDVVENQEFNLSDHSHGSLDSHSGDIAHPLLKDKTLDDTDLNDDNMIAEKLKNLLLEQQRELDELKKKHKQIVSGLLKELPPETREKIVRTCDMEISDYTSESAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XER3</accession>
    <name>A0AAF0XER3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625918</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XER3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000690">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF461">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="309"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH06490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="401" mass="44925" checksum="55B98BFE1637865A" modified="2024-05-29" version="1">MISGYSKLGLFDETLGLFREMQMAGVVPDEVIMSSVVSACGVAGALDVGRWVHLYIDKKMIGKDTMLMTGLVNMYAKCGCINAAKEVFDAMPVRDTKAWSSMIVDFAIHGHAEDALQTFALMEAAKVRPNSVAFLGVLLACAHGGLVSDGRRYWSTMLGYGFEASIEHYGCMVDVLCRANLIEDAYAFVERMPIKPDPAIWRTLLVGCKRNKILEKGELVGERLLKLEPLNAENYTLISNLYASCSQWGKMSLVRKQMKVKGVKVTPGCTSIEVNGVVHEFVMGDWSHPEHKEIREVLEDVRQRVHDSGHEPWISAVLHDVGDKEKENDLCEHSERLAIAYGLFKTKAPTVIRVVKNLRACDDCHEVTKIISKLYMREIIVRDRLRFHKFVNGSCSCGDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFB5</accession>
    <name>A0AAF0XFB5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0626312</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFB5"/>
    <dbReference type="KEGG" id="dcr:108227499"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000039">
        <property type="entry name" value="Villin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000004">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000028">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000038">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF138">
        <property type="entry name" value="VILLIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="10">
        <location>
            <begin position="893"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="740"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="798"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="853"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH06883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="958" mass="107355" checksum="AD0E368A116DFCDA" modified="2024-05-29" version="1">MAVSMRDLDSAFQGAGQKAGLEIWRIENFRPVPVSKTLHGKFFTGDSYVILKTTALKSGALRNDIHYWIGKDCSQDEAGTAAIKTVELDAVLGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFKHAEAEEYKTRLYVCRGKHVVQVKEVAFARSSLNHDDIFILDTASKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGRLMADADTGEFWGLFGGFAPLPRKSAAEDANNISDVPTRLFGIVKGQHEPMDVDSLKRELLDTYKCYLLDCGSEVFVWIGRNTSLDERKSASGAGEELLRTVPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVTVSEDGRSKVAALLKRQGLNVKGLQKATPTKEEPQPYIDCTGNLQVWHVDGDAKTPLSDSNISKFYSGDCYIFQYTYPGDSGEEYIIGTWFGNQSVEEDRNSATSQANKMVESLKFLPVQARICEGNESIQFFSIFQSFLVFKGGFSERYKMYIAEKELPDVTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSHCYILHNGSSVFTWFGNLTTSEDQELVERLLDLIKPNMQSRLQKEGSESEQFWDLLGGKSEYPSQKVGRDVESDPHLFSCSFSNDLKVKEIYNFDQDDLMTEDIFILDCHSDIFVWVGQQVNSKYKKDALTIGEKFLEHDFLLEKLSGQAPIYIVTEGSEPTFFTRFFTWDSTKSAMHGSSFQRKLAIIKNGGTPVMDKPKRRTPVSYGGRSAALPEKSQRSRSVTFSPERVRVRGRSPAFNALAAAFENPNARNLSTPPPVVKKLYPKSGSPDSAKSTPRSSAITALTSSFDKPARETLIPRSVKVSPQATIPKPDTESKENVMSSRIESLTIQEDVKEGEADDEEGVTTYPYDRLTISSADPVKDIDVTKRETYLTSQEFREKFGMTKSSFYKLPKWKQNKLKMALQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XGA3</accession>
    <name>A0AAF0XGA3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0726977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XGA3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="138" mass="16008" checksum="BBA2FF638CF8E80B" modified="2024-05-29" version="1">MAHDNIKELVLGKPHSIFWEIISELVALDKETGRFMQFKSTAPLIKVEVEKVSSLLSYMIALTLEASKFKNDEELLHEANIMIDELNQLSYALDIRILCEENTQMVVKISEGLQSRRPKMEPFLDHLFMQMHQESMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XGZ3</accession>
    <name>A0AAF0XGZ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0727093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XGZ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109">
        <property type="entry name" value="PROTEIN FAM207A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN SLX9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH07660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21406" checksum="49D9FF53BE5DDDA2" modified="2024-05-29" version="1">MGEQVSKQATLGTSADDLFPLFDKYGKVVDVFIPRDRRGKESSRADQKFEKKLQFYAKIRDKVSSLGAQKAIVKKKKLRSRQRKLKAYDLSALSEFLPDEKKETKLQTAVDFKLTCKSRQTLVLKEGKQLNNVLNHPAVKADPLGAIHQHLQNTQPLTDVKQIKKPKKVGKKKAKGKKSKASSAPQSMDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIC8</accession>
    <name>A0AAF0XIC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0727959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIC8"/>
    <dbReference type="KEGG" id="dcr:108194619"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF70">
        <property type="entry name" value="MAJOR ALLERGEN PRU AR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="9"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="157" mass="17043" checksum="0EB15E2AADF50A70" modified="2024-05-29" version="1">MSVVTFSGNHDSPIAPARLFKASITDSKNSMPKIVPEHVKSIDTIEGSGGAGSVKQINLVQGGYLKLKVEALDESSFTYTYSIFEGEALTDKIEKMQFEIKFEPSSDGGSKCTMNTKCFPKGGAELNEDNCKAAKDKVLGLYKAVENYLVQNKDAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPJ0</accession>
    <name>A0AAF0XPJ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 11 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0831328</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in regulating membrane fusion at the tonoplast and the prevacuolar compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Prevacuolar compartment membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPJ0"/>
    <dbReference type="KEGG" id="dcr:108200004"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000278">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 11 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23266">
        <property type="entry name" value="VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="9">
        <location>
            <begin position="394"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="9">
        <location>
            <begin position="561"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="846"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH11833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="936" mass="106074" checksum="8B9C5841FA2E827E" modified="2024-05-29" version="1">MYQWRKFDFFEDKYGGKTSVPDELTGALNCCSSGRGKIVAGCDDGTVALLDRGFKYNYGFQAHTSSVLFLQHLKQRNFLVTIGEDEEISSRLPVVCLKVFDLDKPLNAEGPSTSSLDCIQILRIFTKQFREAKVTSFLVLEEAPPILLIAIGLDNGCIYCIQGDIAREHIKRFTLQVDSTSNINSQLPITGLGFRVDGQSLQLYAVTPSSVSLFNLATQPRSRQNLDHIGCGINSVAMSDRMELIIGRPEAVYFYEVDGRGPCWAFEGEKKFVGWFRGYLLCVIEDHRSGTNTFNVYDLKNRLIAHSIAVKEVSHMLFEWGNVILIMKDKSVLCIGEKDMESKLDMLFKKNLYTVAINLVQSQQANAAATAEVLRKYADHLYSKQEFDEAMSQYTHTIGHLEPSYVIQKFLDAQRIYNLTKYLEKLHEKGLASKDHTTLLLNCYTKLKDVEKLNAFIKSEDGELKFDVETAIKVCRAANYHEHAMYVAKKAGRHEWYLKILLEDLGSYEEALLYIASLEPSQAGVTVKEYGKTLIEHKPEETIEILMKLCTEEDEAAKREASNGTYVSMVPSPVDFLNIFVHHPHPLMDFLEKYTDKVKDSPAQLEIHNTLLELYLSSDLNFPLLSQVDVEQNSDFGVKGSLVGVVPDVKDLKGGKDCERFQKGLRLLKDAWPTDQENPIYDVDLAIILCEMNAFREGLLYLYEKLKLYKEVIACYMQAHDHEGLIACCKRLGDSGMGGDPSLWADLLKYFGELGEECSKDVKEVLTYIDKDNILPPIIVLQTLSKNPCLTLSVVKDYIARKLDQESKLIDEDRRATEKYQEETLAMRKEIHDLKTNARIFQLSKCTACTFTLDLPAIHFMCMHSFHQRCVGDNEKECPECAPEYRSVLDMKKRLEQTSTSQDQFFQKIKNSKDGFSVIAEYFGKGLISKTAIGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPL3</accession>
    <name>A0AAF0XPL3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0831436</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPL3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31314">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31314:SF128">
        <property type="entry name" value="OS11G0106100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="87"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH11940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="339" mass="38793" checksum="3FC96AC23FBEB824" modified="2024-05-29" version="1">MSEADIQSDSSKFDGREEQLDNKTSSESSNNNSSFDLNEVANSEGDECENDVPDPRGEYSDGHVGGSENHECRNESGKKGSVRRYVRSKLPRLRWTPELHRSFVHAIERLGGQERATPKLVLQIMNVRGLNIAHVKSHLQMFRSKKLDDFGQVLSAQTSKTMGGRYYVPNAYGQRLNPLHHFIIKNSDIDTRKSDIHRRLQSLMKNHSQSANVKRLSRSFMCQSFQEKQYTLSRYIENNSNKWTSATAHSPHRNCGLQYPSFNTQRVFETRLEVILHPDWLLFNHETLKPTESLPDLQLKLSQNVGIEDQKIHLNKIPEINTALSLSLSPYSSRQTKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XQW8</accession>
    <name>A0AAF0XQW8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0832102</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May transport inorganic phosphate (Pi).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XQW8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14476">
        <property type="entry name" value="SPX_PHO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034092">
        <property type="entry name" value="PHO1_SPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF4">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTG3</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
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</entry>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="141"/>
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        </location>
    </feature>
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        <location>
            <begin position="19"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="72333" checksum="5659F1548C32828D" modified="2024-05-29" version="1">MPPSPPIPRSVSAPDVFVSNHSSHRSASSAQGTSWLKKTSSLLTYTKKESELTEQSLVALNKRNEALEDKKASHSSPYYKGLTDFTLDINKEKLPSATSLAIDNHEAISFTSSTRSSFIVTMQQLGTFCLFFRSKDEKESSSSASTLSSKQQKDIDSDKGKKEDKSSLVESKSTYILSKEMPMRERVSVITYEPPALQISPPRYKPMTGKSVLPETRSATKASLNEEELSGSVERTYVFTWADKYRPHNLTEFICNREKAMELKAMANSQDISHFIFEGLPGVGKRTMILALLRETFGADKIKARDECMEFYLKGEALSSIEVNVKRSSHHVELNISDLKGYEKHVIVKLINEKCNKSSDSVLESNLDNCQVIVLHEADKLSSDALLYLRWVLEKYKGYNKVFFCCNEVSKLQPIKTLCRVIKLLPPSNEEIMEVLDFIAKEEGIELASKVAETITDNSKNNLRQAIRSFEATWRYNATSQLTEDQEIMTGWEDTIAKVARDIVKEQSPRQLFIIRGELQKLIEYNVSPVFIFKTLVGELKRHLDESSQTHVDILFMEARKREGIDCAAFIHSPTEEMCKRKNDPSRKNVHNFMKIEEFIAKFMSWYKVLLTNTKLQDVKMKGILDDLDPTKGVAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AFS2</accession>
    <name>A0AAF1AFS2_DAUCS</name>
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        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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</entry>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </location>
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    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <end position="205"/>
        </location>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOG87647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="233" mass="26494" checksum="97F9B6FFE6C93639" modified="2024-05-29" version="1">MRSQTDKEEHGEIVALCWSIWQSRNNLVWNQKKCEVNFVVFSTKQYLAEWKNTQVMSTQALYQDVKHGDGAISWVRPKKDVVKITIDAALFAESSKYGIGLLARDDKGDVIQGRSEVFQGVVLQEFAEAIAVKEALSWVKGREWREVVVESDCLAVVQAIRSNVDMRSPFGSLIMECRLMISELNIDLCFIRRSANMAAHCIVRESCSFPGRVFDRRSVPVELESILLADLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Grzebelus E."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF92">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="22"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="26"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="217"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="413" mass="45709" checksum="A2A1D2EB58452766" modified="2024-05-29" version="1">MERQSSLLQIPPPTYGNLITVLSIDGGGIRGLIPSAILEFLESQLQELDGEDARLADYFDVIGGTSTGGLVTAMLTVPDKNKRPLFAAKDIKPFYLEHCPKIFPQKSIWGIFGLLRKLIKLLGGPKYDGKYLHGVVREKLRETKLHQTLTNVVIPTFDIKCLQPIIFSTYEAKKNPCVDARLSDICISTSAAPTYLPAYRFSNCDTEGNVREYNLIDGGVAANNPTLVAMSEVTKQIYDGNQDFYPIKPADYGRFLIISLGTGCAKIEEKYNADSAAKWGVLGWLIHQGSTPLVDVFTQASGDMVDYHISVLTQALHSEENYLRIQDDTLTGIDSSVDISTKGNLEKLVGIGENLLKKPVSRINLDNGIYEEVKDGGTNEEALKRFAKLLSDERRLREIRSPHRTTAENPKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQV2</accession>
    <name>A0AAF1AQV2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ALOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant homeotic and developmental regulators ALOG protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQV2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040222">
        <property type="entry name" value="ALOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006936">
        <property type="entry name" value="ALOG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165:SF2">
        <property type="entry name" value="ALOG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165">
        <property type="entry name" value="PROTEIN G1-LIKE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04852">
        <property type="entry name" value="ALOG_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51697">
        <property type="entry name" value="ALOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ALOG" evidence="8">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="103" mass="11401" checksum="5A2D67267C48F628" modified="2024-05-29" version="1">MSVRRCSGSHVLGFLKYLDQFGATKVHKMSCEYYGQAYSSVACSCPLKEAWSSLQSVVGRLRVAFEEYGGSPLTNPFGSSVVWLYLEEVKVSQAKARGLSYKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AR97</accession>
    <name>A0AAF1AR97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0311789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AR97"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002884">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF90">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG92519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="257" mass="28735" checksum="343CFEA1BE604109" modified="2024-05-29" version="1">MEREHRALISELNEEQLQVYNSIMKSLQRTDGGFFFVYGSGGCGKTFLWKTLISKLRSESMIVLPVASSGIAATLLPGGRTAHSRFKIPIILDDCSTVPGLPTHELKLKVGVVVMLMRNLNQTLGLCNGTRMMVTRLLPNCVECEVISGNFVGTKHFIPRMELFPTDSKLPYKLMRKQMPLQICYAMTINKSQGQSLKNVGLYLPKPVFSHGQLYVAISRVTSPEGLRIYIESEMGLTTNITQNVVYKEVFNNLPCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUX3</accession>
    <name>A0AAF1AUX3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0312705</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG93421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUX3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042162351" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG93421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="38667" checksum="A3448D37D22EAA3E" modified="2024-05-29" version="1">MMLSFLKVFILLLVLPLVLTQPAVNNVLSYGAIGDGLKDDTSSLLKAWEATCKSSASSATMYFPPNHVFLTHPLLLKGPCKAEFVRVEINGTITAPSEPKNWKCGSDNCDTWLHFSHMDGIEISGSGTISGRGQKWWDMKRDNPKMHIVLNGVQTAYLSYLKIEAPGHSPNTDGIYIGESTAVHIQHWTGDDCISIGGGSKKLRINDIICGPGHGISVGSLGKHGDYDDVDDVEIIGAVFTGTTNGARIKTWQGGKGYARNIRFEHILCQNRDNPIIIDQFYCDHEKCEDHDSAVKVSDVKFVNVMGTSKRETAVKIECSKTVSSQEKKKATSYSSNAKGEVRGEVVPKVFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX04</accession>
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        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DCAR_0414921</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AX04"/>
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    </dbReference>
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        <property type="term" value="P:cytokinin-activated signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG95596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="83" mass="9797" checksum="508A1EFEBC9BFAF5" modified="2024-05-29" version="1">MYNILAVDDDRTCLLILKEWLEKWGYQVTTVENAHEALSMLENKSFDLVITDIHMPEMNGFELQERINQNFNLPIICKFMCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX23</accession>
    <name>A0AAF1AX23_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0415483</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AX23"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000330">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021820">
        <property type="entry name" value="S-locus_recpt_kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11883">
        <property type="entry name" value="DUF3403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042095269" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="21"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="24">
        <location>
            <begin position="342"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="515"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOG96152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="833" mass="94103" checksum="E12BCAD6CFA54C34" modified="2024-05-29" version="1">MQLTDIILLWSFSWFTVVNSMAVDTISSNQTIKDGETIVSAGGEFELGFFSPGSSTNRYLGIWYKKISHGTVVWVANRETPLNNSFGMVRVSGNGITLQTVNGSDGLIWSANTSKSLKNPSLQLLDTGNLVLWDEDHNINDSEDFIWQSFDHPGDTQLPGMKFGIDLVTGINRYYTSWKSANDPSLGNFTYKLDSNGFPQFILRKDSVIWSRFGPWIGPAYSGIPNYYQSGYYKYTFVSDGREIYSQFDLINKTPAIMRVVLTPTGEAKILMWNNQQQIWEVNLAQQFTDCDNYGSCGAYGICNINKTPRCECLRGFVPKFPKQWEAADWSNGCVHRTNLVCGTEEDFVKYSSVKLPDTRHSWYNMTINLQECEELCLKNCSCTAYANADVRSGGHGCVLWFTGLIDIRDNIEGGQDIYVRIPSSELGKSGRSKTKRKIKVFVIAGVSTLVLFLVLTLGLFCMTSLYIACRKRKRQLQENLRLSSGIVAWNKIEGEDMELPLFEFESIANATSNFSNDNKLGHGGYGPVYKGMLDDGQEIAVKRLSSNSSQGLDEFKNEVSFISKLQHRNLVALLGCCIDKGERILIYEYMPNKSLDLFIFDEELRNTMDWQKRYNIINGIARGLLYLHEDSKLRIIHRDLKASNILLDHEMNPKISDFGMARSFKANETEANTSRVVGTYGYMSPEYAIDGQFSVKSDVYSFGVLLIEIVSGMKNRLFFHPGHSLNLLGHAWISYNEEKLLQLIDDMIVESCDQTEALRLIQIGLLCVQHDPKDRPAMSEVVFMLSNNTKLAQPKQPGFFMERFLFEVDSISNPNTKSSSNELTITALLARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AXT4</accession>
    <name>A0AAF1AXT4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0416406</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AXT4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434:SF2">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="377"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="468" mass="53376" checksum="6875FBDBF4AE39E2" modified="2024-05-29" version="1">MIINIKQIYQNMNTISKNIIYRILHIIILEWYHNKIKAQVCILIFSGLYNLCVEIDNLIFVWQCRVRKKVRSDEDLCSDIREFMSEFRFPQDHLPSFKELSEHGRQDLAYIVRRRGYKFIKELLATSVNMKTVENDLAGKVDIGAAVNTNQQDEVDDSVEAVNLSSKFPITEDGSDGITYGSEFHMDRKGFTSVESSNESSLQEQVANFIQNGELSMIEAGLICLDSDLSVAEGQTVFESEDTTEELSSNQTDAAKTLDTDNESLSQQVETSSSESSRRKDFFPTDDGLSSADHSDNSDIETRKKENEVEISRLKFILHQKELELSELKQHIEKEKLALSLLQNRAETEISTAQKLVSDKDAELQAAEQALSGLQEVQIQYEGPGESVEVAGSFNGWHQRIKMDPQPSSTPNIVDVMRRRSFTLWRTVLWLYPGTYEIKFIVDGHWKTDPERESVTRGTIHNNILRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZ00</accession>
    <name>A0AAF1AZ00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Condensin-1 complex subunit CAP-D2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0519300</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000403">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="13">
        <location>
            <begin position="77"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="984"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1239"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1312"/>
            <end position="1322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG99944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1355" mass="151454" checksum="638D5F0AD861E886" modified="2024-05-29" version="1">MAPPFIFSPNLETESESDRLSVQTLTDISSFRPSSLEEFVKAVSFDLSDKELFCVEEQDLFDRVYSLVKNFSSLSPSCKFNLVESLRSNFSVLLPNVDSLSRASQSQHDDQIPLVDRIASHRNAFKIYTYFLIQVVLAEESNISTNHSTKVAASSRKKQLINSWNWESQRSRILNLIANSLEINLSLLFGSSDPDENYISFIMKNSFFMFENATMLKDADTKDALCRIIGTCATKYHYTAHSCASILHLIHRHDFVVSHLAHAVAIAEKKYADGSMATYLIREIGRTNPKEYVRDAVGAENIGRFLVELSDRLPKLISTNIGLLVPHFGGESYKIRNALVGVLGKLVSKAFSDFEGEVSSKSIRLRTKQAMLEILLERCRDVSAYTRSRVLQVWAELCEEHAVSIGLWNEVAAVAAGRLEDKSAIVRKSALNLLIIMLQHNPFGPQLRIASFEATLEQYRKKLKELVPDSQPENVLDELPLDSDACNGDGEVDDPRAEARPKEQQDSLTDSCLLEVDNEIIQENVVPDVGNMEQTRTLIASLEAGLCFSKCVSDTMSTLVQLMASSSASDVENTILLLMRCKQFQIDGAESCLRKMLPLVFSQEKSIYEAVENAFITIYLKKSPVETAKNLMNLAIESNIGDLAALEFIVAALVSKGDITSSMLSSLWDFFCFNISGTTAEQSRGALFVLCMAAKSSPAVLGLHLQDIVDIGFGRWAKMDPLLARTACIALQRLSGEDQKKLLSKSGSRLFGILESLITGSGLPENIWYAAADKAITTIYTIHPSPETFAADLVKKVVRSVFAFNGGDDLETVTPNEYTDVLTKLEVTKLSRFLYIVSHIAMSQLVYIESCVRKIQKEKAKKERLVAEGKSVDCNGMTPHDADKENGINAELGLACTDDTILDTLSASAEKEIVSAGSVEKNLIGSCGSFLSKLCRNITLMQKYPELQASGMLALCRLMIIDTDFCDANLQLLFTVVESAPSETVRSNCTIALGDLAVRFPNLLEPWTEHMYARLSDTSVAVRKNAVLVLSHLILNDMMKVKGHIYEMAIRLEDEDERISSLAKLFFHELSKKGSNPIYNLLPDILGKLSNKNLERETFCNIMQFLIGSIKKDKQMEALVEKLCNRFSGVTDTKQWEYISYCLSQLSFTDKGMKKLIELFKTYEHVLCEDLVMDHFKNIINKGKKFAKPELKTCIEEFEEKINKFHIEKKEQEVTARNAEAHQQKLRSLENIKVAVEDGLESTESKTSKAGTDSEVTDEVSTASVPTERIPESEDNSPASSELIEPESSDSEVQSAQVISKEVMKSRAKKATVKDEKCDSEVPPRITRSRRRRWTNNMGNFFLTKWTINMGRREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2P4</accession>
    <name>A0AAF1B2P4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0623808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2P4"/>
    <dbReference type="KEGG" id="dcr:108225983"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH04399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="300" mass="33485" checksum="344B2BE27BB11C3D" modified="2024-05-29" version="1">MVFLVHKLAFHHHHSHHPSEAFLVSLQAFRSEVSNSLAKLMLDSDPGPEFLSIKWLQECFEVLPVINRAFAKLVVEIDYPMTQWEVSSMEEYLGYSLTLLDSLNSISSVLSQLSQARVLLSHALSVNENTLVEFRPLTKIIYKNFGNDLKAINVAGESKRKGVHGKELVLHEALLVLKNIGSWVCSVVASGLCSDAEPYLKMKKSAEKYVLSSLIALDSIVNQKIVQNKEIVKEMKAVNDAVKGHVLANGKGADFSKELQGRLEAIEKAQTGIEGAVDLMFSYVLTVRKELINTLRQRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7A9</accession>
    <name>A0AAF1B7A9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0728760</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH09304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7A9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403:SF8">
        <property type="entry name" value="PHOSPHOLIPASE A(1) DAD1, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="9">
        <location>
            <begin position="183"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH09304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="426" mass="47553" checksum="8D374DFBC2815EA5" modified="2024-05-29" version="1">MRFLVAGASACNLASSKLVVTSFNSTYFVRFNSQSFNITSSWSPMSSVDFCASREAKVKHKWMEYQGVNNWDGLLDPLDEDLKSEILRYGSFVQAAYRSFDFNTSSPGYGSCKFEREMVLTRCGLENCGYKVTKNLRATCGVQMPNWIERAPSWVTTRSSWIGYVAVCDDKDVIARLGRRDVVIAIRGTATCLEWIENLRTTLAPLQKCHVDHQLMVETGFLSLYMSQTDTCPSLRDTLKDEVARIIEMYGDEPLSITITGHSLGAALGTLAAHDINSSLKDLPLITVISFGGPRVGNNCFSKSLETDGIRILRIVNSNDVITKIPGFIINHSSKRNTLVPYGDGMAGWIRKRVHDTKWLYADVGKELRLCCNNPPSFNVGNIATCHDLKTYLELVDGIASSTCPLKDKAKKVLHGTEHEKRLLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7N4</accession>
    <name>A0AAF1B7N4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0729122</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7N4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000200">
        <property type="entry name" value="Homeobox-leucine zipper protein ATHB-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF176">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH09664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="118" mass="14079" checksum="1259D5B6D42CDB49" modified="2024-05-29" version="1">MSDDGSQSVGERKRRLNSEQVKALEKSFELGNKLEPERKMQLARSLGLQPRQIAIWFQNRRARWKTKQLERDYDILKRQFDSLKADNDSLRSRNKQLQGEVRTRLQEDFSITRPQNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WVK9</accession>
    <name>A0A161WVK9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0626466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WVK9"/>
    <dbReference type="Gramene" id="KZM92627">
        <property type="protein sequence ID" value="KZM92627"/>
        <property type="gene ID" value="DCAR_020008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="209" mass="24440" checksum="01CF4A7A93D58F84" modified="2016-07-06" version="1">MASTQSSDLVEADTIYRQHKNILDLIPVVQNHEFSISIQPAKSLHSFSLFRRGCHERFISFVIMGVLKALEYIHARENRVHGNINLNTVFLDEDCEVKLELPVRRNCEDEEQKADIRMVGGLALCLFNDEYRDKFVRDRLVLGCFYGKTRRKELLTGSAVRDFAKFCLYQSHTPTLREVINHEFFKKFGEFDLYQVELKNLLHKKLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X473</accession>
    <name>A0A161X473_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0728252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X473"/>
    <dbReference type="Gramene" id="KZM87233">
        <property type="protein sequence ID" value="KZM87233"/>
        <property type="gene ID" value="DCAR_024367"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="237" mass="26717" checksum="B3F7726E642EFCF9" modified="2016-07-06" version="1">MSSLSWRHHTLIQSLLSRGPLKESEFRSIFTKITANRSDNDRLFHDYLKKINSCLAYVQFELRAFRNQYDGTVYYGGVNNVADEQSKLGTKYTVPQIAFYKGIVPNGGGPESQGVSSQVPAAFRNFSLSQKERTLDELGLCQIIYNPYNLLAERICPGCGPQWDYSVPKSEFVVHEEELQNGNSQNEPPPEPPRRKKQIRSRTEDDTSGSGPSQTCTVKSEMRTTRRSSQLVGASQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X616</accession>
    <name>A0A161X616_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X616"/>
    <dbReference type="Gramene" id="KZM87833">
        <property type="protein sequence ID" value="KZM87833"/>
        <property type="gene ID" value="DCAR_024934"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35495">
        <property type="entry name" value="OS06G0679600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35495:SF1">
        <property type="entry name" value="OS06G0679600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="106" mass="11825" checksum="08722D95C124A4FC" modified="2016-07-06" version="1">MKSRVLNSPSKKKAVCYRYLKPGALARIRDSKKSTANLKKPTSQNALSPSKLVNLSSTSIILETQMIHMAMEGVPFFPALRFSQFSKRGQRKKLFAVAPVFPDIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XSN6</accession>
    <name>A0A161XSN6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin-8-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_017938</name>
        <name evidence="8" type="ORF">DCAR_0520544</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XSN6"/>
    <dbReference type="STRING" id="79200.A0A161XSN6"/>
    <dbReference type="Gramene" id="KZM94696">
        <property type="protein sequence ID" value="KZM94696"/>
        <property type="gene ID" value="DCAR_017938"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223318"/>
    <dbReference type="OMA" id="RRDNAYH"/>
    <dbReference type="OrthoDB" id="1892640at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM94696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH01163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30257" checksum="5E6F8F44AA9B5C3A" modified="2016-07-06" version="1">MVKLSNNLVGILNIITFILSIPILIAGVWLSRQGTTECERFLEKPVIAIGVFLMLISIAGIIGACCGVSWLLWTYLLVMFLLIVLLFCFTVFAFAVTNKGAGEHLSGKGYKEYRLGDYSNWLQKRVNNDKNWRKIKSCLQDSKVCKSMTEDADTTPVEKFYLERLNSIQSGCCKPSDSCNFTYVSPTNWTKTTEPAPNSDCALWNNDPKVLCFDCQSCKAGMLDNIKSDWKRVAVVNVIFLICLIIVYSIGCCAFRNNRESNSWKRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XZ09</accession>
    <name>A0A161XZ09_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_010761</name>
        <name evidence="2" type="ORF">DCAR_0312231</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XZ09"/>
    <dbReference type="Gramene" id="KZN02007">
        <property type="protein sequence ID" value="KZN02007"/>
        <property type="gene ID" value="DCAR_010761"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN02007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="61" mass="6947" checksum="D02BEAE07E0BCAA6" modified="2016-07-06" version="1">MELMDEALDAGWGAPHGPGLAAQFMDEMGFQVGLRVRSKLKLKYMTSFGRMKLQICLRMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y6L1</accession>
    <name>A0A161Y6L1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PSII 6.1 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0206606</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the psbW family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y6L1"/>
    <dbReference type="Gramene" id="KZN05037">
        <property type="protein sequence ID" value="KZN05037"/>
        <property type="gene ID" value="DCAR_005874"/>
    </dbReference>
    <dbReference type="OMA" id="IWSINAC"/>
    <dbReference type="OrthoDB" id="2017665at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042549">
        <property type="term" value="P:photosystem II stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009806">
        <property type="entry name" value="PSII_PsbW_class2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34552">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34552:SF12">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07123">
        <property type="entry name" value="PsbW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG87382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="13685" checksum="AC944A3B3407FBB0" modified="2016-07-06" version="1">MAAITGCYLASGVTRAGPAGLLQKAPTVRSVLGLPVMPKAGKVRCSMEVKSRSDSKVGMGASLAVMATAAAMSSPAAMALVDERMSTEGTGLPFGLSSNLLGWILFGMFGLIWSLYFVYASGLDEDDDSGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZLR2</accession>
    <name>A0A161ZLR2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_025277</name>
        <name evidence="12" type="ORF">DCAR_0729027</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZLR2"/>
    <dbReference type="STRING" id="79200.A0A161ZLR2"/>
    <dbReference type="Gramene" id="KZM88202">
        <property type="protein sequence ID" value="KZM88202"/>
        <property type="gene ID" value="DCAR_025277"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193761"/>
    <dbReference type="OMA" id="GSEAYCR"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    <sequence length="238" mass="27234" checksum="1DD520F75239B133" modified="2016-07-06" version="1">MDFPLASTRSKRSVAQDLILRKYSPASFELPKHVVIVMDGLKEPSLALLEWVLKNFAVETHCTVTLLRVMPWLTFPFSGRTWDDIWMLDFEDLVTNQEETQCKNDVYLKLQPLIELCRQYNVIPQVKPLMGFPIHLLVGEQITSLHATLAVIDRHHAKKNINYYAARVPCNILAMNQDGEFDMIKARSGIDVDDYSIGDSPAPTPKLIVSHRFLNKFLKQKEKTGKEEKSLYTVCSTP</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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</entry>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AI00"/>
    <dbReference type="STRING" id="79200.A0A162AI00"/>
    <dbReference type="Gramene" id="KZN01201">
        <property type="protein sequence ID" value="KZN01201"/>
        <property type="gene ID" value="DCAR_009955"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211151"/>
    <dbReference type="OMA" id="NIFEERY"/>
    <dbReference type="OrthoDB" id="264917at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF8">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="3">
        <location>
            <begin position="63"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN01201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG91983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="274" mass="31280" checksum="4E3BAEDC1A5D76E8" modified="2016-07-06" version="1">MALPQLLPSHSSSPYLKSTQQNPRFLFSSPPSILLRRRKPNPLRCSASSFSEKHRANSPTNDVVELPLFPLPLVLFPGAILPLQIFEYRYRMMMHTLLQTDLRFGVIYTDAATGTADVGCVGEVIKHEKLVDDRFFLICKGQERFRVTKLVRTKPYLVAEVNWLEDRPKEGEEDLEACANEVENYMKDVIRLSNRLNGKPEKEAQDLRRNLFPTPFSFFVGSTFEGAPREQQALLELEDTAMRLKREKETLRNTLNYLTAASAVKDVFPSSGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AJ67</accession>
    <name>A0A162AJ67_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">spermidine synthase</fullName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_010607</name>
        <name evidence="13" type="ORF">DCAR_0312062</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AJ67"/>
    <dbReference type="STRING" id="79200.A0A162AJ67"/>
    <dbReference type="Gramene" id="KZN01853">
        <property type="protein sequence ID" value="KZN01853"/>
        <property type="gene ID" value="DCAR_010607"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215298"/>
    <dbReference type="OMA" id="HIYNEMI"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000003">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000048">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF25">
        <property type="entry name" value="SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="11">
        <location>
            <begin position="56"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN01853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG92786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="359" mass="39552" checksum="DE1376B2B324A01A" modified="2016-07-06" version="1">MAGDVGSGMEDQTLMDVKGRNGTSTDQDEAKIPSCCVKARSLDPEFEANCHSTVVSGWFSEPQSSADKSGKCIYFNNPMWPGEAHSLKMDKILYKEKSKYQEILVFESAAYGKVLVLDGILQLTEKDECAYQEMIAHLPLCSIASPKNVLVVGGGDGGVLREICRHSSVEHIDICEIDNMVIDVSKRFFPELAIGYEDSRVQLHVGDGAEFLRNAPEGKYDAIIVDGSDPIGPAQELVEQPFFESAARALRPGGVFCSMAESMWLHTHLIDDMYGACQNAFKGSVHYAWTSVPTYPSGVIGFLICSTEGPPVDFRNPANPIEMLDGALNYRRELRFYNSEVHTAAFALPSFVKREVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ARA9</accession>
    <name>A0A162ARA9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0206254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ARA9"/>
    <dbReference type="Gramene" id="KZN04723">
        <property type="protein sequence ID" value="KZN04723"/>
        <property type="gene ID" value="DCAR_005560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG87034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="363" mass="40985" checksum="4C33C115C2284414" modified="2016-07-06" version="1">MVDQFTQDSTNVPRGNAQQMEGKKKWQLDKILKHWFLLDYDLVEAINKGNHSLMPMAIARVNYLIGKVPAKLLAEGINGEEEALWSIHELLYNNGCWEKANNLVIADYDVSAIEKSTDPVLLFLNAYTHLIHPNTRVGALKGKREDVRMALNQIHYSSIEAARQSSQVGVTIKNNRTKFYSQLQEARQFISAHSNLVEPSVLKDAMSVALATTKAERAAPNEPQQQEEAALQETVLHTSNWIPREADSSCSLPKSSTDLYISEVDNNVSEDGTVCSEVLAELQNLKVQVKRGRPRKYKKPQLNKHFKLPRKKKTRGEGLKQTTHYFLNAGFDEAEAIYETGRLMGLLPSDSKERSIELIKENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B617</accession>
    <name>A0A162B617_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="11">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_003129</name>
        <name evidence="15" type="ORF">DCAR_0103304</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="11"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B617"/>
    <dbReference type="STRING" id="79200.A0A162B617"/>
    <dbReference type="Gramene" id="KZN10473">
        <property type="protein sequence ID" value="KZN10473"/>
        <property type="gene ID" value="DCAR_003129"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202978"/>
    <dbReference type="OMA" id="RNLICTC"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000025">
        <property type="entry name" value="Glycine cleavage system P protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000007">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00711">
        <property type="entry name" value="GcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="12">
        <location>
            <begin position="81"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="12">
        <location>
            <begin position="519"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="851"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN10473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG84123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1038" mass="112957" checksum="C2BEBE56EC2A53D4" modified="2016-07-06" version="1">MDRARKLAGRAILRRLISDSKQQRPNELLPPVIYTPSRYVSSLSPYNFNTNLGKSVGSFNGSLSRSLNVESLRPSDTFPRRHNSATPDEQSKMAQFCGFESLDSLIDATVPKSIRLDEMKFNKFDGGLTESQMIEHMKMLASKNKVFKSFIGMGYYNTAVPPVILRNIMENPGWYTQYTPYQAEISQGRLESLLNYQTVITDLTGLPMSNASLLDEGTAAAEAMAMCNNILKGKKKTFIIASNCHPQTIDVCKTRADGFDLKVVVKDLKDFDYSSGDVCGVLVQYPGTEGEVLDYGEFVKNAHAKGVKVVMASDLLALTLLKPPGEFGADIVVGSAQRFGVPMGYGGPHAAFLATSQDYKRMMPGRIIGVSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQALLANMAAMYAVYHGPEGLKVIAQRVHGLAGILAAGLKKLGTVEVQELPFFDTVKIKCADSKAIAEAAYKSEMNLRIVDKNTLTASFDETTTLEDVDKLFKVFAGGKSVPFTAASLASEVQDVIPSGLVRETPYLTHPIFNSFHTEHELLRYIHKLQSKDLSLCHSMIPLGSCTMKLNATTEMMPVTWPSFTDMHPFAPTEQAEGYQEMFNNLGDLLCSITGFDSFSLQPNAGAAGEYAGLMVIRAYHMARGDHHRNVCIIPVSAHGTNPASAAMCGMKIVTIGTDAKGNINIEELKKAAEANKKNLSAFMVTYPSTHGVYEEGIDEICKIIHDNGGQVYMDGANMNAQVGLTSPGWIGADVCHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPYLPSHPVISTGGIPAPNEAQPLGTISAAPWGSALILPISYTYIAMMGSGGLTDASKIAILNANYMAKRLENHFPVLFRGVNGTVAHEFIVDLRGFKNTAGIEPEDVAKRLMDYGFHGPTMSWPVPGTLMIEPTESESKAEMDRFCDALISIREEIAQIEKGKVDANNNVLKGAPHPPSLLMADKWTRPYSREYAAFPAAWLRSTKFWPTTGRVDNVYGDRNLVCTLLPTNGVEEQEAAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SSR1</accession>
    <name>A0A164SSR1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0727207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SSR1"/>
    <dbReference type="Gramene" id="KZM86585">
        <property type="protein sequence ID" value="KZM86585"/>
        <property type="gene ID" value="DCAR_023719"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195491"/>
    <dbReference type="OMA" id="CENANYE"/>
    <dbReference type="OrthoDB" id="647907at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010683">
        <property type="entry name" value="DUF1262"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31050:SF3">
        <property type="entry name" value="OS08G0412800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31050">
        <property type="entry name" value="OS08G0413200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06880">
        <property type="entry name" value="DUF1262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="401" mass="46025" checksum="6F3170DFACE1464C" modified="2016-07-06" version="1">MYVARLLSHYTKNPESLSLLPEGPNSGYLVIQDEEAETSSWFGSSKNHYLKSLPFPYNNILTIRTYVQNIQDLSTVDKVVLIPVINQPLSCNRYYAIDAGNHRENYKGKGFTCLKDEQVTCPHCKTRTPSKTIYDLTRRPVDPLGIYQQFHIYLGGTTEGGNGSFYANSVAHDAFPPDFLKMKRWCIRTRKAKNSKLAEALGLDSDLRARIADTSIQISCKSSEAVKVGMWYCPFIFIKDRTLAYQMKKSMYYEMTLEQRWEQIFECKNNSINCNAVMITAPVQKEVVLVGGSVAVWDAKNVVDKTIWFKAIETKGEEVSVGLSLEIFERMNWEQERAGYVGEGRRLVTFNRVEKFGGGSEGWRKFGCYVLVERFVLRRMDGSLVMTYDFKHTHQVRCIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UWB3</accession>
    <name>A0A164UWB3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0622828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UWB3"/>
    <dbReference type="Gramene" id="KZM89457">
        <property type="protein sequence ID" value="KZM89457"/>
        <property type="gene ID" value="DCAR_023180"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225635"/>
    <dbReference type="OMA" id="LICSKHA"/>
    <dbReference type="OrthoDB" id="755532at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33237">
        <property type="entry name" value="F2P16.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33237:SF46">
        <property type="entry name" value="OS01G0606100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="14837" checksum="04B611202EAAE8F7" modified="2016-07-06" version="1">MNSPMPKKISPAPEKLAAGFLTSVVSLWLICSKHATRTSKKIAPKKVMARLSHKAIQFRQRKSGGEAKTGGEDGGVWRRAILMGDKCEPLDYSGVIYYDSEGNRLSEVPVRSPRASPLPSYAYKSEKEKSRLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VD62</accession>
    <name>A0A164VD62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_022469</name>
        <name evidence="12" type="ORF">DCAR_0623692</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VD62"/>
    <dbReference type="STRING" id="79200.A0A164VD62"/>
    <dbReference type="Gramene" id="KZM90166">
        <property type="protein sequence ID" value="KZM90166"/>
        <property type="gene ID" value="DCAR_022469"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224581"/>
    <dbReference type="OMA" id="FWHVISD"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000012">
        <property type="entry name" value="Mannan endo-1,4-beta-mannosidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853833" description="mannan endo-1,4-beta-mannosidase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="10">
        <location>
            <begin position="30"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM90166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH04283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="436" mass="50163" checksum="285FCE91983477ED" modified="2016-07-06" version="1">MRTWALLFLLLLFLFDDHGINLPQVSASNEFIKSRGGHFMLNGDFYYANGFNAYWLMDVASDLSQRNKVSSVFREAVSNGLTLARTWAFSDGGYQALQVSPGVYDERVFQGLDFVIHEAKRHGIKLILSLVNNYEDYGGKNQYVKWARDQGQNITSDDDFFSNPLVKGFYKKHVNTILTRRNSFTGVPYKDDNTIMAWELINEPRCTSDPSGRTLQAWIKEMASYVKSVDKKHLLEVGLEGFYGQSAPEDKQFNLNFKTGTDFVANNQIPGIDFATIHMYPDQWLPGSDEKTQQTFSEKWIGKHIQDAETILQKPLLVTEFGWERSGFDRKTRERLFDTVFSDIYLSARRGGAAAGGMFWQLLTEGLDSYRDGYEIVFSESPSIASLISDQSKQLVQIREKYSRQRHMKKGGQTRDKVEIRQVYSRQRHIRKIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W3Y2</accession>
    <name>A0A164W3Y2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0624909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W3Y2"/>
    <dbReference type="Gramene" id="KZM91255">
        <property type="protein sequence ID" value="KZM91255"/>
        <property type="gene ID" value="DCAR_021380"/>
    </dbReference>
    <dbReference type="OMA" id="YTSFLAC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
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    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="30"/>
            <end position="76"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH05492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="152" mass="16443" checksum="2CB4273C285D0E48" modified="2016-07-06" version="1">MAPGILVLAAWNPKKPLILLPSIAHNIQLSSDFNAISGTSMSCPHASGLAALLIGAHPEWSPAAIRSAVMTTVNPLDNSQHKIKDTETHLEIATPLTMGSGQVVTNRALNPRLYTMQQHMTMPISSSRQFYKKIKFLPSQDPVSAAQTHLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WL04</accession>
    <name>A0A164WL04_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625643</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM91925">
        <property type="protein sequence ID" value="KZM91925"/>
        <property type="gene ID" value="DCAR_020710"/>
    </dbReference>
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    <dbReference type="OMA" id="QITYYTA"/>
    <dbReference type="OrthoDB" id="1426517at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH06220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="195" mass="22217" checksum="07F2032A765182F6" modified="2016-07-06" version="1">MSSKQKKLIRWSIISLLIALFILLLIILIIWVVLQPKKPQFILQDATLYAFNLSSTPPYFLSSNLQVTISSQNPNKRIGIYYDKLDVYAAYRTQQITYYTATPPDYQGHKEVIEWSPFLYGTDIPVAPSNGESLRQDESNGAIWLMIKLNGHVRWKVGSFTSGRYHLRVNCPAFIPLSKASNAKYQLVANCKVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WVX3</accession>
    <name>A0A164WVX3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_020299</name>
        <name evidence="13" type="ORF">DCAR_0626128</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WVX3"/>
    <dbReference type="STRING" id="79200.A0A164WVX3"/>
    <dbReference type="Gramene" id="KZM92336">
        <property type="protein sequence ID" value="KZM92336"/>
        <property type="gene ID" value="DCAR_020299"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228043"/>
    <dbReference type="OMA" id="YWWGYES"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:metallochaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="68"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="214"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="326"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM92336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH06700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="421" mass="45563" checksum="3DEC035BEBD8D9E8" modified="2016-07-06" version="1">MVKEQVESKGVQNLWIESDSDKSSGVKFDGSCVSLISDPVLGTQGSGELSMPQHKEVLNDKSSSGGNLGLTERAFSAAGAAFLSAIIVNPLDLAKTRLQAQAAGVQYSHPLSNLTSRMAFSGPNMMFADLRCSTSCTRAGVHGTVSICPPDCFQYKGTLDVFYKIIRQEGISRLWRGTNAGLALAVPTVGIYLPCYDIFRNYLEDFTSKNAPIMTPYTPLVAGSLARSLACISCYPIELAKTRMQAFKEINRSSKPPGVWKTLIEVISSVKSTNNLQSLQNYRALWTGLGAQLARDVPFSAICWGTLEPTRRRLLRVVGENSSAIGVLGANFSAGFVAGSLAAAATCPLDVAKTRRQIEMDPTRALRMTTRQTLMEVWRDGGMRGLFTGVGPRVGRAGPSVGIVISFYEVVKYCLHHHYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X012</accession>
    <name>A0A164X012_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="12">CPR</shortName>
            <shortName evidence="12">P450R</shortName>
            <ecNumber evidence="12 13">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0626327</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in the biosynthesis of the antimalarial endoperoxide artemisinin. Acts as a redox partner for CYP71AV1 which catalyzes the conversion of amorphadiene to more oxygenated products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="12">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="12">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="12 13"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X012"/>
    <dbReference type="Gramene" id="KZM92511">
        <property type="protein sequence ID" value="KZM92511"/>
        <property type="gene ID" value="DCAR_020124"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228017"/>
    <dbReference type="OMA" id="DANKMAR"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06204">
        <property type="entry name" value="CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000003">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000023">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023208">
        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0274">FAD</keyword>
    <keyword evidence="2 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 12" id="KW-0288">FMN</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12 13" id="KW-0521">NADP</keyword>
    <keyword evidence="9 12 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="95"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="475"/>
            <end position="478"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="493"/>
            <end position="495"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="617"/>
            <end position="618"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="623"/>
            <end position="627"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH06898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="697" mass="77887" checksum="5453E71D3A02A5D4" modified="2016-07-06" version="1">MQSESMEVSPVDLLTSILKIDSVESMTMLLENREMMMLLTTSFALLVGFGLVLMWRRSTTKSVKSFEPAKIVIPKFEMEEELDDGKKKVSIFYGTQTGTAEGFAKALAEEAKARYQNATFRTIDLDDYAAEDDEYETKLKKESLVFFFLATYGDGEPTDNAARFYKWFCEGKERGEWLKNLEYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRLVPVGMGDDDQCIEDDFTAWRELLWPELDQLLLDDDAMAVATPYTAAVLEYRVQLYNQSDTSSPLDRSMSKLNGHAVYDAQHPCRANVAVRRELHTPASDRSCTHLEFDISSTGLAYETGDHVGVYTENLIEVVEEAERLIDISPDTYFSIHTDNEDGTPLSGGSLPPPFPPCSFRTALTRYADLLSSPKKSALVALAAHASDPSEAERLRFLASPVGKDEYAQWIVANQRSLLEVLAEFPSAKPPLGVFFASVAPRLQPRYYSISSSPRLAPSRIHVTCALVHETTPAGRIHKGICSTWMKNAVSLEDSHVSSWAPIFVRQSNFRLPSDSKVPIIMIGPGTGLAPFRGFMQERLALKETGAELGSAVLYFGCRNRKLDFIYEDELNQFVETGAISELVVAFSREGPAKEYVQHKMSQKASEIWDMISQGAYVYVCGDAKGMARDVHRMLHTIAQEQGALDSSKAESFVKNLQMSGRYLRDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Z7K1</accession>
    <name>A0A164Z7K1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0521415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Senalik D."/>
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            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_012697</name>
        <name evidence="7" type="ORF">DCAR_0314391</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CIK7"/>
    <dbReference type="STRING" id="79200.A0A166CIK7"/>
    <dbReference type="Gramene" id="KZN03941">
        <property type="protein sequence ID" value="KZN03941"/>
        <property type="gene ID" value="DCAR_012697"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213706"/>
    <dbReference type="OrthoDB" id="1592334at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF94">
        <property type="entry name" value="PROTEIN ATAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
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            <begin position="21"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZN03941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG95089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="200" mass="22914" checksum="0848CEFBA274F1B2" modified="2016-07-06" version="1">MSNNLPAAAKVIVEEEIGDSLPPGYYFLPSAAQLIEEYLLQKILDLPMASNIVKIMDFVELIDPDQLNLEEFKYCKDKEGYYITKKAERKANEKTIIKTLTGHWKEYKSNIPISNNSQIVGYKSTFTFYQADGTETAWKLNEYTSNPDIVPVDALTDTVRAKIEEYVACRIHIKKVKLAEQVIYNEETVKDEDEDEDGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CQJ2</accession>
    <name>A0A166CQJ2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0205569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CQJ2"/>
    <dbReference type="Gramene" id="KZN04199">
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        <property type="gene ID" value="DCAR_005036"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208359"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="148" mass="16566" checksum="B042C9A5AE9AC0C1" modified="2016-07-06" version="1">MPSREDETVNDSIDMEEEEYSDDDSCMRIYPSHGAHHYKPQDLIDFSTRAITQYNQKKGSSYMFVELVEGKMLLGDTVITEYKAQETNGVIKTFRASATTTVRRASAKTTYALSDPNLRIRITRVPDSGFLIVSSDSDSDSDSDSGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DTN4</accession>
    <name>A0A166DTN4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Short-chain dehydrogenase TIC 32, chloroplastic-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_006501</name>
        <name evidence="5" type="ORF">DCAR_0207326</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DTN4"/>
    <dbReference type="STRING" id="79200.A0A166DTN4"/>
    <dbReference type="Gramene" id="KZN05664">
        <property type="protein sequence ID" value="KZN05664"/>
        <property type="gene ID" value="DCAR_006501"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208082"/>
    <dbReference type="OMA" id="APHIRRY"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF114">
        <property type="entry name" value="OS03G0115700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN05664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG88093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="36328" checksum="7EAAEC5841523A8A" modified="2016-07-06" version="1">MLDTLKYLLGWKGPSGYGSKSTADQVTSSVDLRSSTAIITGATSGIGAETARVLAKRGARLVLPARNQKAAEETKARIQSEFPGAEVLIMPLDLSSLSSVRNFVSEFESLNLPLNLLINNAGKFSHKHAVSEDGIEMTFATNFLGHFLLTKLLVEKMIETAKTTGVEGRIVNVSSSIHGWFSGDLIPYLEHITRNKSDYDATCAYAISKLANILHTKELANRLQGMGANVSVNCVHPGIVRTRLTRDREGFLTDLVFFLTSKLLKTIPQAAATTCYVATHAKTKGITGKYFADCNEASPSKLATSSIQAARLWSASEAMVTSPDPKFGAYPFNGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EHF6</accession>
    <name>A0A166EHF6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0208359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="252" mass="26594" checksum="4B56EA4709ECE7B2" modified="2016-07-06" version="1">MAMIIFIVLSFFLQGALGEIICEQLPVQMCTFSVASTGNRCVLESYVSNAGSTELQCKTSDVFAENVAQWIEDDHCVEVCGVDRKTVGISSDSLSEPRIIASVCSTACYDNCPNIVDLYYNMALAEGSYLPNLCKAQNAMSRREMSQVNAWAPAAANAESDKSDAWAPAPVGAIPIIPILFPPTPAPVSVIPVIPILFPPAPAPSHAESAESVASAPPSTIPFVPLPLAPTPLVAPPFPISWPPAPVPASDN</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    <sequence length="356" mass="40067" checksum="FE4F5FF871A8521B" modified="2016-07-06" version="1">MPPQLHPTAATSTAITVHRAAAKPRQPPPENTTSPKLTTSLYQTEIGLFALTWSKNVMSRSLHVHYTSHDVVSDQSFPSFTLHLKPFLFWKKHGSRKLAVSPSPHAKNPRIFYDFSNAKFGSRPEPESGFYIAVVLDGDMILLIGDSPKQAYSRTKSSSSSSTSNPNPNPNQSMILKREHVRGAHRVYATKATFGDKTRNILVDCRLVNNKSSLILTIDNKRVLRIKHLEWKFRGNERIQVDGIQIQVSWDVHDWLFDEKDKDDGHALFMFKFEKLGFSDDDDDSSSLWSQQRQSCGIGYEARKLKKGVLTSSTSSCSSSSLSSASSNSVMEWESIEENQLKLPSGFSLLLYAWKN</sequence>
</entry>
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        <name evidence="4" type="ORF">DCAR_0101498</name>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Daucus</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
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    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GFC7"/>
    <dbReference type="Gramene" id="KZN08895">
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    <dbReference type="GO" id="GO:0016740">
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GFP7</accession>
    <name>A0A166GFP7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_001561</name>
        <name evidence="9" type="ORF">DCAR_0101593</name>
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    <organism evidence="8">
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <location>
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        <source>
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    <sequence length="164" mass="18559" checksum="083C9736AB0473E1" modified="2016-07-06" version="1">MASSSGITTTSGSLNTDLYQQHLQQLLDQRKSKRKMSNRESARRSRVRKQKHLDELMAQLAQLRKANNQLVMIMNVTAQGLLNVEAENSVLRAQVAELEARLESLNEIIGFLDIENEGFGINEEFCYNAVNDSADMFMLNVNSSMSYMYANQLPIMASADMLQY</sequence>
</entry>
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    <name>A0A166GHD6_DAUCS</name>
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            <fullName evidence="8">SAM-dependent MTase DRM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_001625</name>
        <name evidence="10" type="ORF">DCAR_0101522</name>
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="9">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN08969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82358.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="159" mass="18890" checksum="7B09CC3E75510528" modified="2016-07-06" version="1">MMNLHCLIHEQVASYQQEALPWAKYWWQKWDKRTKFNWIVMNNGWPNDIDNIAKVLNNSGFAPPEHIRKDVLRRCKRYNYVWVGKNKVTRLEPHEIEYVMGYPDDHTSVLNTTYRHKCLKNVFQVNTVAYHLSVLKNLFPDGIKVLSLFFGIGRAQVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GKC7</accession>
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        <submittedName>
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    </protein>
    <gene>
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    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOG82507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_001706"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS11G0490100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21729">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="32768" checksum="92FDDEFB07B87E01" modified="2016-07-06" version="1">MRSKTKYPVNNKFLLVIGVFFAFFLLFVLRSSFSSSKNISSPVTQALPKVLATISDADPTSCSPTCSKIPPSVAKALVHYTTSTITPQQTLKEISVTSRILEKKSPCNFLVFGLGHDSLMWSTLNFGGRTIFLEEDEAWIAQIKQRFPTLESYHVTYDSKVHQADGLMEVGKGNECTAIGETRYSMCQLALKGLPNEVYDTKWDLIMVDAPTGYYDEAPGRMSAIYTAGMIARNREEGAETDVFVHDVNRVVEDKFSMAFLCEGYMKKQEGRLRHFRIPSHKNDRDRPFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HWY1</accession>
    <name>A0A166HWY1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0103329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant homeotic and developmental regulators ALOG protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HWY1"/>
    <dbReference type="Gramene" id="KZN10492">
        <property type="protein sequence ID" value="KZN10492"/>
        <property type="gene ID" value="DCAR_003148"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208448"/>
    <dbReference type="OrthoDB" id="10690537at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009299">
        <property type="term" value="P:mRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040222">
        <property type="entry name" value="ALOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006936">
        <property type="entry name" value="ALOG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165:SF2">
        <property type="entry name" value="ALOG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31165">
        <property type="entry name" value="PROTEIN G1-LIKE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04852">
        <property type="entry name" value="ALOG_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51697">
        <property type="entry name" value="ALOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG84148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="177" mass="19922" checksum="9F608D7DF12B59AA" modified="2016-07-06" version="1">MSAAVAAAAAAALANRRNNSPSATTANKNNYNTNQHYLSYSTRPPRHNRYEMQKRHDWNTFSQYLKSRHHHLHPPPSMRRCSGSHVLDFLKYLDQFGETKVHNISCKYYGETHSTACSCPLKQAWSSLEAVVGRLRVAFEEHGGAPETNPFGSPAVWLYLREVKECQAKARGLGFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ISC8</accession>
    <name>A0A166ISC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">L-ascorbate oxidase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_004093</name>
        <name evidence="8" type="ORF">DCAR_0831987</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ISC8"/>
    <dbReference type="STRING" id="79200.A0A166ISC8"/>
    <dbReference type="Gramene" id="KZN11437">
        <property type="protein sequence ID" value="KZN11437"/>
        <property type="gene ID" value="DCAR_004093"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202009"/>
    <dbReference type="OMA" id="GPLHVVP"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13872">
        <property type="entry name" value="CuRO_2_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034271">
        <property type="entry name" value="CuRO_2_AO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF510">
        <property type="entry name" value="L-ASCORBATE OXIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007875435" description="L-ascorbate oxidase homolog" evidence="3">
        <location>
            <begin position="26"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="36"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="163"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="384"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN11437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="62887" checksum="E3BD53F3FB20AD41" modified="2016-07-06" version="1">MSRVKFELVLGLLLISCLFLRRVHGEDPYLFYTWNVAYANLSPLGVPQQVITINGEFPGPMINGTSNNNVWVNVFNNIDEPISFHWHGIQQRKNSWQEGLPGTTCPIPPGTNYTYKFQVKDQIGSYFYYPSTALHKAAGGYGSIRIHSRDLIPIPFDPPEAEFAVMIGDWYSKSHVELKKQLDGGRTLAKPDGVLINGKAGKGDNKDEPMFNLQPGKIYRFRFCNVGFKYSLNVRIQNHKMKLVEVEGSHTVQNMYDSLDVHVGQCYSVLVTMDQEPKDYFMVVSTRFSKSVLTSTATIRYVGGNGPASTDLPQAPTGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGGINITRTIKLASTAGKADGKLRYAFNGLSHTDPETPLKLAEYFDVPDKVFQYDNIKDDPPAVDDENVKIEPVVLNSTFRNFVEIIFENHEKSTQTFHLSGYSFFAVGIEAGKWSPDKRKNYNLLDALSRTTIQVYPKGWAAIMLTLDNAGMWNVRNMALERSYLGQQFYISVLSPERSLRDEYNLPENFPLCGIVKTMPLPPPRYSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IU72</accession>
    <name>A0A166IU72_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFZ2"/>
    <dbReference type="Gramene" id="KZM82098">
        <property type="protein sequence ID" value="KZM82098"/>
        <property type="gene ID" value="DCAR_022672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM82098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="17192" checksum="6B9471D4D7E5DA42" modified="2016-09-07" version="1">MKKNPLGSSPPSTNHALYAVIVIGLNGDIEQHHWQRRMKLRITTLIRFQEYTHAVHQLTDTMLRHVVMVLAYFGQAAYLMKNKEHVSDAFYRAIPGAMKIAGPTVHGQFKEKKTGNSSYSDQPSNSSYFGFPVYCPLKVPDVVVDKRMQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0934888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YH15"/>
    <dbReference type="Gramene" id="KZM82461">
        <property type="protein sequence ID" value="KZM82461"/>
        <property type="gene ID" value="DCAR_030030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF2">
        <property type="entry name" value="NUCLEIC ACID DIOXYGENASE ALKBH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH15350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="14970" checksum="03804058DEB44A70" modified="2016-09-07" version="1">MNLQICGDVDQLDDRADNSEIPRDRSGGSSTAKYRRTRVKAKKILIVNRGLPIVSILIGESGDFLYGDQRDVDKAEKIVLESGNVLIFGGQSGHVYHGAASILPTTALQALLQESDLRPGRLNLKVVAVMLLFQVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YK59</accession>
    <name>A0A175YK59_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_028831</name>
        <name evidence="4" type="ORF">DCAR_0830473</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM83747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH10996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YK59"/>
    <dbReference type="Gramene" id="KZM83747">
        <property type="protein sequence ID" value="KZM83747"/>
        <property type="gene ID" value="DCAR_028831"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197909"/>
    <dbReference type="OMA" id="RSENGEW"/>
    <dbReference type="OrthoDB" id="1918594at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
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        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF11">
        <property type="entry name" value="F-BOX PROTEIN CPR1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="2">
        <location>
            <begin position="142"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM83747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH10996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="398" mass="45977" checksum="CD954DC459776BC0" modified="2016-09-07" version="1">MSNKSGAELVGFEFETDDLDYLMKRKASNNITEKEDDHSLLPEMYEEILVKLPAQYLYQDLRLVCKSWNNIISRKNFILDNFSRTKPVFLVQCDTLCRRSYCMEIDEQDLSYRMPEFGFDCVGRFKASCYGMLLMESRIAWNVREPELQVLNLVTKCCLTLPECPSGCAHKECGSGLGFDPCTNFFKVVHIYDMKVGFEIFTIGGSSNEWKTVPGPWKDLSKRSLLSSGWDDPVSVNGRFLHWNVLSYEYIVSMDVSDEKCIKIKLPYSVESFTDESEFNLVELGGYLACIYSASRTQMEIWILQDFQRKLWLKKHSVHAKMIISSINPVPSFTQICPVGCTSNGVVVFTHTKRQRRMYIYDMKRKAMKKHAAKTKIDKLIVHRSSLFRLQNGSCYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YNY9</accession>
    <name>A0A175YNY9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_027253</name>
        <name evidence="9" type="ORF">DCAR_0832290</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium sensor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calmodulin family.</text>
    </comment>
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        <property type="protein sequence ID" value="KZM85325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF53">
        <property type="entry name" value="CALMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
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            <end position="150"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM85325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH12782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="150" mass="17086" checksum="47201E23C8EA0A10" modified="2016-09-07" version="1">MADLLNQEQIVEFQEAFSLFDKDGDGCITIEELATVIRSLDQNPSEEELQDMISEVDADGNGTIEFVEFLNLMAKKMKETDVEEELKEAFKVFDKDQNGFISATELRHVMINLGEKLSDEEVEQMIREADLDGDGQVNYDEFVKMMMTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPK2</accession>
    <name>A0A175YPK2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">DUF668 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_027291</name>
        <name evidence="5" type="ORF">DCAR_0832252</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPK2"/>
    <dbReference type="STRING" id="79200.A0A175YPK2"/>
    <dbReference type="Gramene" id="KZM85287">
        <property type="protein sequence ID" value="KZM85287"/>
        <property type="gene ID" value="DCAR_027291"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197642"/>
    <dbReference type="OMA" id="YGNSYID"/>
    <dbReference type="OrthoDB" id="2018987at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DUF3475"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007700">
        <property type="entry name" value="DUF668"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371">
        <property type="entry name" value="BNAC09G50660D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31371:SF2">
        <property type="entry name" value="PLANT_PROTEIN (DUF668)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11961">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF11961"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM85287.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="64141" checksum="2BFB3AB272BDCA5D" modified="2016-09-07" version="1">MVVETWLLKMTSQVSANFKHALLESSKKSNAKKQEVKESTGILCFEVANLMSKTVLLHKSLGDKEISKLKSELLNCQGIKNLISCDEAFIFELALCEKMEELNRVAGVVSRLGKKCNLPALQGFEHVYGDIVSGVLGVKDLGFLVKDMEAMVRKMERFVNLTGALYGEIAVLRELEITTKKFQQSHLEETRKAFEQKLMWQEQDVKHLKDVSLWNQSFDKVVEMLARTVCTLYVRICNVLGSESGQRNDGLRQLKADCGVKFGRIDVKLENSVVIRRGLRKKNGGDMSGLTERNRMEKRKSNVRPKVEEQLFRAEHLDILCGMGRGGLFKECLNLGSPGTKVDDDNECSGLEDRSVAGGEKRNNLKHSGGVVSSQTSSTFSFSEANCSRSTLKSSFMDYATPSTIGGSGLALRYANVVIVVEKLLMYPHLVGEEARDDLYFLLPTNLRMSLKTGLKSFKEDFAIYDASLAHDWKERLDQILQWLAPLAHNMIRWLNERNIEQQQVIKRTNVLLLQTLYFADRVKTEEALCEVLIGLNYICHYEHQHDALLDCSSSFDLDDCVGDWQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPQ1</accession>
    <name>A0A175YPQ1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPQ1"/>
    <dbReference type="Gramene" id="KZM85704">
        <property type="protein sequence ID" value="KZM85704"/>
        <property type="gene ID" value="DCAR_026874"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197352"/>
    <dbReference type="OMA" id="TFLLMTM"/>
    <dbReference type="OrthoDB" id="2306007at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049202">
        <property type="entry name" value="DUF6817"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37391">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37391:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20680">
        <property type="entry name" value="DUF6817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="430" mass="48572" checksum="CD20118ED9E320FC" modified="2016-09-07" version="1">MPTSSISSSQPSQDQYLKTLLESARPFLRGELEFVDPKLPSLVAVLRSVGAGECWHKHGSFLDHLVDIYKILKLWKAQDSVCLCGLFHSAYSNSYVNLAIFDPSTGRDTVRDHVGEAAERLIHLFCIVPRQPLIHDDLLFKYSDSELVEHLKVSEVSLRNAKEKGVFDGDESWRKKVQALVPASGITIKHIKTGEDVLVSRRVVAVFLMMTMADFSDQLFGFQDALFDNSNGRLEFSGNSFTALWPGDGKPGLWLNSISRMGAIYSLLVREEEIFIEERKRGNDNGLDRDRDEDLELVIPPVFAKCTKVLDAGDQKAARELYWEGVCDMSKGELGLDGVEKVLRMSVEKNPYVGEPYVVLAQICLSQGRYEEAEKEAERGLTLLLEWGSPWDKRMSWEGWVAWARVLVMKAREKSWPQTSWGILNLGLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQQ7</accession>
    <name>A0A175YQQ7_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_027127</name>
        <name evidence="3" type="ORF">DCAR_0832437</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQQ7"/>
    <dbReference type="STRING" id="79200.A0A175YQQ7"/>
    <dbReference type="Gramene" id="KZM85451">
        <property type="protein sequence ID" value="KZM85451"/>
        <property type="gene ID" value="DCAR_027127"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198467"/>
    <dbReference type="OMA" id="KCDMYAG"/>
    <dbReference type="OrthoDB" id="770116at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33670:SF1">
        <property type="entry name" value="OS09G0416300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33670">
        <property type="entry name" value="SPLICING FACTOR, PROLINE- AND GLUTAMINE-RICH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM85451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH12928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="14731" checksum="39290DBE33B1B9EA" modified="2016-09-07" version="1">MGTEVLRSQDIIREAPASFHHRKTYHGYGNSGPKVNRKQVRPEHRRNSPAPRKSEMLGQLVILKRGEALGAKIKAGPDPKQVRLGVQDTYAGSAFSMSPSPSSLPLPTFFTKGGVKAVDDSAATRDLRRLLRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQU7</accession>
    <name>A0A175YQU7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0833182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQU7"/>
    <dbReference type="Gramene" id="KZM86094">
        <property type="protein sequence ID" value="KZM86094"/>
        <property type="gene ID" value="DCAR_026484"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197197"/>
    <dbReference type="OMA" id="EVVTYLM"/>
    <dbReference type="OrthoDB" id="5314041at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF40">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH13671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="671" mass="74830" checksum="3FBE713637F223B2" modified="2016-09-07" version="1">MPPTYFPLRWESTGDQWWYASPIDWAAANGHYDIVRELLHFDTNLLIRLTSLRRIRRLETVWDDEAQFDDVAKCRSQVAQKLLLECENKKGQNSLIRAGYGGWLLYTAASAGDVGFVKELLERDSLLVFGEGEYGVTDILYAAARSKNSEIFRFLFEHSVSLRSSSHGGEWEVNRDEISSVFKGEMMNRAVHAAARGGNLDILREFLRDCLDISEFRDDQGCTLLHTASGRGQIEVVKDLLASYDMIDSKDKHGNTALHVAAYRGYLAVVDIIISLHPSTTFITNNDGDTFLHMTVAGFRVPGFQRADRQIELMKQLISGQVVDMQSIINARNNEGKTALHMAVIENINSELVELLLTSEAIDLNIRDDDGMTPLDLLKQRPQSASSQLLVKQLKSLGGISHCHDEKSKCTVISHLKLQGIGGSPGTSFRIPDADIVSFTDTESSNVFNHDPSTATYNTCSGELSHYDSLDKPNSLGNKKFGSVNYATRHLKILLHWPKKKITKAHNTATEDDDSLDSYSRINAGSDYNQCSLRQRFSRNTSVLNKKRILPFQSTLPSPSAKQKFTTGLTHGVLQITPKFGLESLSSRYSDSSKSSPISFNQLNRHDSEKDGCISSSNSIVNGEKSQRYSRHGSFNKRLMNQYLCFGAQGINVDDSVSCTRQAHSALSFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W1L5</accession>
    <name>A0AAF0W1L5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W1L5"/>
    <dbReference type="KEGG" id="dcr:108197374"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006734">
        <property type="entry name" value="PLATZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF46">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04640">
        <property type="entry name" value="PLATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25430" checksum="347119178FE8D6BC" modified="2024-05-29" version="1">MVKESSGVMAKPAWLEGLLSETFFDACELHQTTRKNEKNILCLICCQSLCPHCLPHHHSHPVLQVRRYVYQDVIRLEDLEKLIDCSHIQPYTINKAKVIFLKPRAQSKSSYKGSGNNTCFTCERFLQEPFHLCSLSCKVQNMVNQGDDVSSITYGFDESEFHFSQFQGLEMDHSSPSNLENPSHEFEGSSCNGHDRGSSEYPQQMMLSFHNRRKGAPQRSPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W3P5</accession>
    <name>A0AAF0W3P5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor SPIKE 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0100671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202588"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08679">
        <property type="entry name" value="C2_DOCK180_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000005">
        <property type="entry name" value="Guanine nucleotide exchange factor SPIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF76">
        <property type="entry name" value="LD20667P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="469"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1387"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG81521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1838" mass="207444" checksum="F35E7D9185A4D091" modified="2024-05-29" version="1">MENSSPNGHRFRRISRQSFAGSLNLDPLLDENLEQWPHLSELVQCYRTDWVKDDNKYGHYESIGPISFQNQIFEGPDTDIETEMHLANARQNKIDDTDDDIPSTSGRQFTDAASKSSSLNVLKHLGESPLPTYEPVFDWETERSTIFGQRIPETQLAQYASGLKISVKVHSLSFQAGLVEPFYGTICLYNKERREKLSEDFIFSALPTEMQEASSSYEPRGIFYLDTPSASVCLLIQLEKPATEEGGVTPSVYSRKEPVHMTEREKQKLQVWSRIMPYRESFAWAIIPLFDSNINSSPGGPASPSSSVAPNVSVSSSQDASDPVAKLDGKLGYSSGNSVVVEVSNLNKVKEGYTEDSLQDPKRKIHKPVKGVLRLEIEKLQAGPVDFENASEGGSIDHEDQITDSRFAKCPSNGSDGPQNGHSKVNYYEGKELPRNGSIALGNTDLNTDDFQAFDFRRTTRNEPFLQPFHCLYVYPITVSLSRKRNLFIRVELRKDDGDARKQQPLEAMHSREPGASLQKCAHTQVAVGARIASYHDEIKVSLPAIWTPSHHLLFTFLHVDLQTKLEAPKPVVIGYASLPLSTHAQLRSEISLPIMRELIPHYLQDGGKERIDYLEDGKNVFKLRLRLCSSLYPISERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLQPILNMLLHLIGNGGETLQVAAFRAMVNILTRVQQESVDEAERNIFLVNYVDYSFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQTRLFYHNLPLGEDIPPMQLKESVFRCILQLYDCLLTEVHERCKRGLSLAKRLNSSLAFFCYDLLSIIEPRQVFELVSLYIDKFSGVCQLVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWDHDDLSLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLVGQILDEMPVFYNLSSVEKREVLIVILQIIRNLDDASLVKAWQQSIARTRLFFKLLEESLVLFEHRKPADSMLMGASSRSPVADGPVSPKYSDRLSPAINHYLSEASRQEVRPQGTPENGYMWQRANSQLSSPSQPYSLREALAQAQSSRIGASTQALRESLHPILRQKLELWEENLSAAVSLQVLEITEKFSSTASSHSIATDYGKLDCITSIFTSFFSRYQPLAFWKAMFPVFTSVFQLHGATLMARENDRFLKQIAFHLLRLAVFRNHNIRKRAVVGLQILVRSSFSYFTQTARLRVMLTITLSELMSDVQVTQMKSDGTLEESGEARRLRNSLEEMADESKSGNLITECGLPENALGTIPDALAEKKWSWSEVKILANSIILALDASLEHALLASVMNTDRYAAAESFFKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVGVAGVVMQALVSRNDGVWGNDHITALRKICPMVSNEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCANILELVIPVYKSRRAYGQLAKCHTMLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDKKEYVYREPRDVRLGDIMEKLSHIYESRMDGNHTLHIIPDSRQVKSEELQPGVCYLQITAVDPVMEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSDGDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQDFHTQLVNGFQSLTAELSHYIPAILSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4U5</accession>
    <name>A0AAF0W4U5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UTP23 sensor motif region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0102445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="2">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="206" mass="23517" checksum="689FD8231068D730" modified="2024-05-29" version="1">MRLKRQKRHGRTLRFFTACFGFGDPFKVLCDCTFIHHLVAHDINPVHTALSNVLGTQFISRCDHDKSKSAVDCITEIIGEIMQITSLLLHRMLICNFLLVEPLSNSQRHFAKSAEEEHLHLKDLELKMLNMKGYSMDRNCMGSEPVKLFGEKKKADLRDKGPNPLSCKKKKTKGNQNNAPKKVMFRNSHITISLSRQIENSTAIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7W7</accession>
    <name>A0AAF0W7W7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0102488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7W7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF379">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="534"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG83313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="580" mass="65328" checksum="615E888A071276F5" modified="2024-05-29" version="1">MRSLRYVGKHPNVVLLETCKTLKQIKQIHTQMLINGLFTDLKMLGQFVSAVALKQPEYIDYSIQVLEQCERPPLAVLNSLMRAHYKIAKPCKSFYFYSRIVKSSHNLKPDNYTFTFLIRTCAQLFVEETSMAVYGSVIKYGFDVDPHVRSSLISMFAELGLLGFSKKIFLEIFEPDSVTSTAMVSASAKSGDLVSARELFDRMPDRDLATWNAMMAGYVQWGQAREALDLFYLMQSEGVKVNEASMVSVLSACAQFGALDQGRWAHNYIEKLRLRVDVTLGSALVDMYAKCGDMDKAMEVFWSLKEKNVYTWTSAMGGLAVNGFGNECLELFARMKHAGVEPNEVTFVSLLIGCRVVGLVEEGQRYFESMSNVYGLQPSIEHYGCMVALYSSAGRLDEAISFINSMPIKPHVDAWGALLNACKIYKNKKLGKLAFKKIVELEGGARGTYLNFMRTGRGEVSQVTEVWDCSTTEANGEVHKFFIGDKSNPRQAEIKKKMEEISQRLKLAGYVANTISVLFDIEEEEKENATCVPVKNLRVCSDCHHVTKMISEIFDREIIVRNTNSSYHFTNGECSCNGYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WA04</accession>
    <name>A0AAF0WA04_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WA04"/>
    <dbReference type="KEGG" id="dcr:108204618"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF91">
        <property type="entry name" value="PROTEIN ENHANCED DISEASE RESISTANCE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="1">
        <location>
            <begin position="48"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="287" mass="31803" checksum="61D2F280D4481B68" modified="2024-05-29" version="1">MSLVKRKLKRRCSGIEQCAKLLEDDWKYEVIYGGSLKHVDLKHGSNGWASPPGDLFSLRSQTYFTKKAKAPSGPWLLQPAGVDWLRSYDKLDHVLGRTDNRVMNALRKAQSIGKNMKSFVMAVNLQMPGRENHSAVFYFSTDDPIASGSLLDRFINGDDAFRNSRFKMVNRIVKGPWLVKNTVGNYSACLLGKALSCSYHRGDNYLEIDVDIASSAIATTILHLALGCVTAVTIDMGFMVEAQCEEELPERLFGAVRISEMEMSSASFVETRSFNKKVGGAGSLSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAL4</accession>
    <name>A0AAF0WAL4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Suppressor of forked domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0105104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAL4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000064">
        <property type="entry name" value="Putative pre-mrna-processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000159">
        <property type="entry name" value="Tetratricopeptide repeat (TPR)-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG85911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="92557" checksum="ECC0668FCF544E98" modified="2024-05-29" version="1">MGDSETVVTQTKSVMAYTSTEYASTDNYNENAASDAVTSDAGTVGNVPSKISAGSGDMEVLTDVMVTDNTSEHTQNLTDENGNQNLAKEEVPTTTNDVSENVANSASESMDTTHVSAYNSLNGIDATEAKNVVSDVGENGTTAVANEFMSEAPALSAEEERLWSIVRANSLEFDAWTALIEETERVAEGQISKIRKVYDAFLAEFPLCYGYWKKYADHEERMGSSDKVIEVYERAVQGVTYSVDMWLHYCVFAINTYGDPDTVRRLFERGLVYVGSDFLSYTMWDKYIEYEYTQQEWSRLAMIYTRILENPNQQLDRYFTSFRELVAHRPLSELRTAEETAAAAAAAVGATGQEIEGEVHPNAVESPKPASAGITEAEELEKYITIREEMYNKAKEFQSKIIGFETAIKRPYFHVRPLNPVELENWNNYLDFIEAEDDFQKVVKLYERCLIACANYPEYWIRYILHMEARGSTDLAENALARATQVFVKRRPEIHLFAARSREHSGDIPGARAAYQLVHAEIAPGLMEAIVKHANMEHRLGKAEDACSLYEQAIAIEKGKEHSQTLPQLFAQYSRFVYLVCGKAEKAREILEQALEICQPSKSLLEAVIHLESIQSTPKRIEFLDSLINKFIAPNPDNPSAASFIDREELSSIFLEFLDHFADAHSVKKAEDRHAKLFFVHKSTSESKKRHADDILQSDKAKLAKSGYSSSPSVMGAYQSTQNQWPAGYGAQPQAWPQATQAQAQNWNPAYPQQQAAYGGAYGNAYAQPQVQTSVPQAAAATAYGAYPPAYTVQQAYPQQTYVQAATPAPLAPAQQPAAAVAPQAYYGTYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCK4</accession>
    <name>A0AAF0WCK4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCK4"/>
    <dbReference type="KEGG" id="dcr:108215591"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF299">
        <property type="entry name" value="OS02G0653400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="40703" checksum="E4F9FF4F413F0C09" modified="2024-05-29" version="1">MANSSNHMVQYEGFLTVVPHRPTFPRPSVRISTEKDREAGLLRQCFRMILCYEKASSDDSGWMVAGWTKTSIGIALSEKPVLAGRLRRAEDGGLELVSNDSGVRLVEARIALRLDEFGGLRNKEEERKQLVYWKSIDDQDTQFSPLMYIQVTNFKCGAYAIGISCSILLADPFSMSSFLKRWSEIHMNLVTESLSQKLPLFYLPNFGKPNSTQVYSPNYEPAKETSRQTLIFKFANLTVDNEMQRIVALSCIDKVNRRLGKKVTSDFSLLVKNPNNDMLKIDTISVKEVLSLESFKNLTGINSKDYSWGEYIDADGINIAKGNKPTETVCWISSPDEEDLVMIISSESNMTVIVTVSTTNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WIM7</accession>
    <name>A0AAF0WIM7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0209307</name>
        <name evidence="8" type="ORF">DCAR_0209308</name>
        <name evidence="9" type="ORF">DCAR_0209309</name>
        <name evidence="10" type="ORF">DCAR_0209310</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WIM7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF136">
        <property type="entry name" value="PROTEIN STRICTOSIDINE SYNTHASE-LIKE 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041856353" description="Strictosidine synthase conserved region domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="147"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG90069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="328" mass="36170" checksum="5475850D6B4EFA8E" modified="2024-05-29" version="1">MISIFGFMITTIMLFVSSVVSQPRISFTKILLPPNTTSPESVAVDRRGNGPYVTLADGRVLKWLGPDFGFMDFATTSPDRTKDVCDGTLNPDLIFQCGRPLGFSFDTQTDNLYIVDGVLGLYVVGPNGGQATRLASSAEGITFRFLNGVDVDTFTGIVYFTSSSTTYDIRNITQPGFQPDKSGRLLKYDPSTKQVTVLLRQLYVPIGPAVSLDGSFLVFSEYGNKRILRYWLKGLRANTVEVLFTVPGYPSKIKRTSIGDFWVPVNIINPATFIATPMGFRFNSFGTVVQKVDFSSQYANQNITVLNEQNGVLRVGCRTVNFIGIYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WK48</accession>
    <name>A0AAF0WK48_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sec-independent protein translocase protein TATB, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0310606</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across the thylakoid membrane. Involved in delta pH-dependent protein transport required for chloroplast development, especially thylakoid membrane formation. TATC and TATB mediate precursor recognition, whereas TATA facilitates translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WK48"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.3310:FF:000003">
        <property type="entry name" value="Sec-independent protein translocase protein TATB, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33162">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33162:SF3">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATB, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01506">
        <property type="entry name" value="TATBPROTEIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="26917" checksum="E50F98FE6C233222" modified="2024-05-29" version="1">MSTAAMLSTQICRSSYVKTPTLCTLSTSSYSISTTTNINFSWIHQLGPTSFSQWSCLRPLGTSISQLRFRRKFKRKGVYASLFGVGAPEALVIGVVALLVFGPKGLAEAARTLGKTVRAFQPTIKELQDVSREFKSTLEAEIGLDDIRNPIQSTTNVKTNPLEKITAEDSQMKVDPNGSPSTKKAYSSEDLLKITEEQLKAAATVEQKQIIAPGEDQSEAQTRPQDTVQEDATEGPPSLPQESKSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKU7</accession>
    <name>A0AAF0WKU7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0310753</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKU7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="541"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="11">
        <location>
            <begin position="585"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG91504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="774" mass="89190" checksum="6741F014F3424C2E" modified="2024-05-29" version="1">MTAPSIDASFYNHSVGNDTVININESEIILTKMQLDSSYDPHFVTDCPAAHRSSWSEIPTSSDLYGQKSPGKVSFIEKLRRRGRHGGSAFSSNKSFPRESELVSMRSKSRLIDPPEQNDSFKRIMKSVECIEEGSDVHDDDPYVEEDFPEEFKMIKFSKWAVIQFFCMIFIIAALVCSRFLNIFNENKLRGLELWRWSLLLLALLCGRLVAGWVIRVVVFFVELNYMLRKRVLYFVYGLRHAVRNCIWLALILISWRFVLVDRVENLVHGKALNYVTKAWVCLLVGTLIWFVKTLFVKVLASSFHVSTFLDRIQDALFDQYVIETLSGSPLVEHHLEPVEEDQIMAAVPQLQNAGATVPPDLRTTVSSTNTKPKRMAKTRKSFPSFKSSRFSTVATKEEKEGITIGHLHRWNQKNISAWNMKRLMNIVKKGTLSTLDEQTGEDESAVQIRSETQAKFAAKKIFCNVAKPGSKHIYQEDLMKFMRDDEALKAITHFEGASGWKGISKKELKNWMVNTFRERRALVLSLNDTKKAVNKLHQMLNIFVALFIGAIWILILKVASIHFFVLLSSQLLLVVFVFGNTCKTMFEGIIFLFVMHPFDIGDRCEVDDVQLVVEEMNILTTTFLRYDNQKICYPNSLLSNKPISNYYRSPDMGDAIDFSIHISTPVEKIALMKEKITSYIEKNSDHWYPAPMIVLRDIEDMNRLKISLWLSHRINFQDMGKRWARRALVLEEMIKTFKELDIEYRMVPLDMNVRSMPAMASNRLPSVWTSHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM72</accession>
    <name>A0AAF0WM72_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310064</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM72"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09323">
        <property type="entry name" value="TDT_SLAC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030183">
        <property type="entry name" value="SLAC/SLAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269:SF2">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="552" mass="61960" checksum="B23C0D10581DC65F" modified="2024-05-29" version="1">MPPSPIEDHLEGKKRVLFRDDCELISSSGVSHSPLSTDIPKLNQPIFHSQPIPKGSVLNVALASGKSPDRPPKNPMGKTLADKRYNSFKTFSGKLERQLSSLRGRVNETGSDFPRTTEMENVPVHRYFDALEGPELDTLRPSEEILLPEDKQWPFLLRYPISSFGLCLGVSSQAIMWKNLATSTTMGFLHVSQKVNIVLWCLSIVLFTIVAFTYILKIVFYFEAVRREYYHPIRVNFFFAPWIALLFLALGVPPSLTKDLHAALWYVLMTPILILELKIYGQWMSGGQRRLSKVANPSNHLSVVGNFVGALLGATMGLKEGPIFFFAVGLAHYTVLFVTLYQRLPTNETLPKELHPVFFLFVAAPSVASMAWARIQGSFDFGARIAYFIALFLYFSLVSSLAYLNHEPTFQVDCNSLSVFQFSLAWWAYTFPMTGAAIATLRYSLEVSNVVTKSLSILLCTVATFTVTALLVTTIFHAFVRWDLFPNDIAIAISDKRPKTVRKWYHRRNGSTDIKDSEQYLKFTDSNGKDIEASLKSSGSGATEENTHVSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPG7</accession>
    <name>A0AAF0WPG7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polyadenylate-binding protein</fullName>
            <shortName evidence="12">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0312936</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPG7"/>
    <dbReference type="KEGG" id="dcr:108212638"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000651">
        <property type="entry name" value="Poly(A) binding protein cytoplasmic 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000239">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000285">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="110"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="201"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="304"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="14">
        <location>
            <begin position="546"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG93650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="634" mass="69724" checksum="8A05DD906E571F56" modified="2024-05-29" version="1">MATATVPSPTAVSGGGTAINNVSLYVGDLDKNVGESQLYDLFSRVGQVMTIKVCRDLIRNTSLGYAYVNFTNPQDAAHARDVLNFTNVNGKPIRIMYSQRDPSMRKCGFANVFIKNLDKSIDNKALLETFTAFGTVLSCKVALDSNGKSRGFGFVQFDQEESAQSAIKQLNGMLMNDKQVYVGFFIRKQDRCRTSGSQKFTNVYVKNLPDTVSDEDFKNMFEKYGLITSAVVMKDADGKSRCFGFVNFQNPDNASAAVENMNGFLCNEKVLYVGRAQRKSEREAELRAKFEQERISRFEKLKGANLYLKNLNDTINEEKLKDLFSKFGTITSCKVNLDPQGISKGSGFVAFSSPDDAAMALNEMNGTMVERKPLYVAVAHRKDERRAHLQAQFAQIRAPGTLATLPGGMPGFHPGPPRHPQQSLYFGHGTPALIPPQLTGYGFQQQLVPGIRPGVPQNFVIPPYQFLRQGPSEQRMGQRRNGNAQHMQQQWMPRNANNGFSRYMANGRSGDATVVPQAVMGQMMPLPIDGSIRAPLDSQHAGPVPKPTTLASALASANPANQRVMLGEHIYPLVQCLENEHAGKVTGMLLEMDQTEVLHLIESPDALKKKVAEAMDVLRVANTAAASDVAEISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPK7</accession>
    <name>A0AAF0WPK7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPK7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47780">
        <property type="entry name" value="PROTEIN SET DOMAIN GROUP 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47780:SF1">
        <property type="entry name" value="PROTEIN SET DOMAIN GROUP 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="140"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="567" mass="63081" checksum="FE9098AB6140CE0D" modified="2024-05-29" version="1">MEMRSIGDIDNIGQDLTPPIYPVTFSLYNTHLLSHCSVCFSPLSLNQQHSPSSSLSPPLYCSSLCSSHDSPLRDPIRTLISSTHSDSSDLRAALRLLHRIIKEGAKAIAKMRGSRSASDHDCVMEEAALCVVITNAVEVQDGNGVAVGIAVYDPAFSWINHSCSPNACYRFSEFDDDSRMLIAPAASFGGECSGGNVFATRWERYGPKIGVRNIKAISKGEEITIAYTDLLQTKETRQSDLWSKYRFICHCRRCNTLPPAFVDHALQVRLNGALNIDRVVGKLTEYMDSAINEYVKFDGAESCCEKLEHVLAYGYNDGQSLDEGELQEKIRLQPLHHLSLNAYTVLASACKALGSRLLDLSSNSLDDQLNAFDKSRISAAYSLLLAGATHYLYLSESSLIISVANYWTAAGRSLLSLATSTVHNISMERCYPIPNISPLKLRCNNCALKDKFEASSVRRHVQNLDFEQISRDFFNCITDIVPHVWMYLIQGSHYLKNIKSPIDFAWLGNTKDSKASDIGVNMGSDQVMRSLSRCEQEKWYDNRNLNFFQLGAHCLLYGDLVLKICCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRT7</accession>
    <name>A0AAF0WRT7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0313502</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRT7"/>
    <dbReference type="KEGG" id="dcr:108211854"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF15">
        <property type="entry name" value="CYTOCHROME P450 71A2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0492">Microsome</keyword>
    <keyword evidence="9 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG94209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56376" checksum="C6215FA5BCC28B1B" modified="2024-05-29" version="1">MVPDQQLLFLSLCTLISVLVFLYVWFSTRTTGKNLPPSPRKLPIIGNLHQVNQDPHVALRSLSQKYGPVMQLHFGSVPVLVVSSADAAKEIMKTHDLAFANRPDSTIWSRIFYKGKDVAFAPYTEYWRQVKSICVLQLLSTKRVRSFHNVREQEVGLLVEHIRDSGSDIVNLSDLTYTLLSNVVSRIALGKKYTNSTGDGEENSFRELFQNIAQLIGYFSFSDYIPWLYWIDSLSGLKGRVEKAANEIDAFLEGVIRDHSTALSNGASSDDLLKTLLEIQKQDSDSAFSIDNDSIKGVILNMYFDGTDSTSAVLEWTMAALIKHPDIMYKLKNEVREIGRGKSRINGDDLEQMPYLRAVIKESMRLYTPVPLLVAREAMQDVKVMGYDIKAGTQVLINAWAIATDPAVWDNPEKFIPERFLNSPLDYKGLHFEFIPFGAGRRGCPGIQYAMAINELALANLVHVFDFALPDGKKFEDLDMASETGMTVHKKSPLLVIATPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WW25</accession>
    <name>A0AAF0WW25_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0415050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WW25"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000169">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000403">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG95723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="513" mass="58429" checksum="D5470CABE09F12AF" modified="2024-05-29" version="1">MERQVAIIGAGISGLAACRYCKAKGLNPIVFESESSVGGVWRKTIRTTKLQTPKHFYQFSDFPWPASVTEDFPAQSQVFEYLQSYASHFDLLRHIRFRSQVVSIRYDGPPDAEMQAWSLWGGTNDHSNTKGKWHVTVKDQQALLTEVYQVSFVILCFGKFSNLPNIPEFPNDKGPEVFHGKVIHSAEYASMDHTAAANLIRDKKVAVVGFQKSALDIAMECSSVNGPEHPCTVVYRTKHWGIPYFLPFGIPLLYPYLNRFSELMVHKPGESLALSLLVSILTPLRWGISKYIERYMTSKFGLAKFDMVPEHSFLKEINSCSIAILPEDFYNRVEKGGIKLKMSQAFCFSEKGILFDDEVESEDVDMVILATGFKYFEKLKDIFVSPTFQSHIANASGLYRNCLHPRVPQLAVIGFSESISNLHTSEMSCRWLAELLDGTFSLPSIKEMEDDVKEWDKYMKKYCGSYSTKWCIGGLKIWYNDQLCKDMGWISKRKGGFFAELFEPYSPLDYIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WXL3</accession>
    <name>A0AAF0WXL3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WXL3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.80:FF:000003">
        <property type="entry name" value="AT-hook motif nuclear-localized protein 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039605">
        <property type="entry name" value="AHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500:SF57">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="9">
        <location>
            <begin position="160"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="292"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="354" mass="36030" checksum="B98307E66FF40A4B" modified="2024-05-29" version="1">MSGSETGTVMTTPFSSVAGHNSPSSQNMRLSYTSDGAATYTPLPNAPPQYQLPSDPAVEAPPITAVQPPQQQNMNVNVMGEVVKKKRGRPRKYGPDGSVGLLTHSSAPPANFSGGPLSSPPLQSPPSQNVVASASGSSPKKGRGRPPGSKKKQHLNNALASPGIGFTPHIIDVKVGEDVSSKIMAFSQNGPRAVCILSANGAISNVTLHQAATSGGTATYEGRFDILSLSGSFLLSEVGGQRSRTGGLSVSLSGPDGRVLGGCVAGLLTAASPVQVIVGSFIVDGQKGSKTINQMEPLSSPPQLNPVSGAPGPNSPQSRGTLSESSGGPGSPLNQNTGALNNSNYQGMSSMPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY70</accession>
    <name>A0AAF0WY70_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Macrophage migration inhibitory factor homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0415415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY70"/>
    <dbReference type="KEGG" id="dcr:108217932"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050178">
        <property type="term" value="F:phenylpyruvate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.429.10:FF:000004">
        <property type="entry name" value="Tautomerase/MIF superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001398">
        <property type="entry name" value="Macrophage_inhib_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954">
        <property type="entry name" value="D-DOPACHROME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954:SF25">
        <property type="entry name" value="LS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01187">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG96085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12759" checksum="A9C4D1ED11CC0CC7" modified="2024-05-29" version="1">MPCLYISTNLKLDGVDTDSVFSEATKAVARIIGRPEKLVMVLLKGSVGISFGGNKEAAAYAELISMGGITRKVKRELISTLGTILQQNLSIPPARFFLKVFDTTLMRPSGDDIIVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1J1</accession>
    <name>A0AAF0X1J1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhamnogalacturonan endolyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0418262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X1J1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10317">
        <property type="entry name" value="RGL4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10316">
        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000033">
        <property type="entry name" value="Rhamnogalacturonate lyase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051850">
        <property type="entry name" value="Polysacch_Lyase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010325">
        <property type="entry name" value="Rhamnogal_lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018:SF10">
        <property type="entry name" value="RHAMNOGALACTURONAN ENDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06045">
        <property type="entry name" value="Rhamnogal_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="3">
        <location>
            <begin position="379"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="2">
        <location>
            <begin position="463"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG98916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="75641" checksum="1D6CCB66668B9C91" modified="2024-05-29" version="1">MLGLWLSSCITCGKKLSLISKPDMGMLLHAQDDYIVIDNGVIQITLTNPGGNLTRIQYSGIDNVLETHNKELNGGFWDLNWSEAGSRKNRGKFDTITGTSFKIIVENEKQMELSFTRPWDPSDQGEHAPLYIDKRFVILRGVSGFYSYAIYKHLKDMPAFNLNTSRIAFMLSKDKFHYMAVADNRQRYMPLPDDRLPGRGEELAYPEAVRLVDPVEPEFIGEVDDKYQYSIENKDNRVHGWICFDPPVGFWQITPSNEFRNGGPLKQDLSSHVNPTTLAIFLTSHYAGEDLLVKLGAGEEWTKVLGPVFIYLNSVPSAANALSLWEDAKEQMIKETNCWPYNFLASDDFPCSDYRGTLRGRLLVYDRYIAEFDAPAALAFVGLAAPGDAGSWQRESKGYQFWTKANECGEFTIGNVRAGSYNLFAWVPGFIGDYQCDIEICITPGCDIDMGDFVYEPPRDGPTMWEIGIPDRSAAEFYIPDPNPKYINKLYVNHPDRFRQYGLWERYAELYPDEDLVYTVGVSDYTKDWFFAQVNRKIGDRTYKTSTWQIKFEVLNIERSKNYKLRLALASAHHSDLQVRINDPEGDNPLFSTGEIGDDNAIARHGIHGLYWLFTVDIPGDQLVEGEINTIYLTQSKNSSPFQGIMYDYIRLEAPSPENTKAASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8D1</accession>
    <name>A0AAF0X8D1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0622678</name>
        <name evidence="6" type="ORF">DCAR_0622742</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NET family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8D1"/>
    <dbReference type="KEGG" id="dcr:108225568"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF26">
        <property type="entry name" value="KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="4">
        <location>
            <begin position="13"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="167"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH03282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH03345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="330" mass="38047" checksum="136A4C487FBA43B0" modified="2024-05-29" version="1">MVLKKGSAAAVFSSSSLHDLSNQSSRSFFPTSRPSWLLVSIADMDKRMKMVCSNSIKEQKADSFAEQAETYYQKRPQLLSLLQDLYNGYLTLADRYCRALAKQHHSPSMTPVSVHQSSDDEVCSNEVSDAESSISFQAPVKPHVVMNDADSIVAEVVIKNVEYEIVIDEMNEEEKQWNESSRKIELQKSLLEVLETERLVLLNENARLEHRVETLVDENKALGEESYFMKTKASELARCVLKLRDDYKANMFSSKVEDLQAQIYVLEKRNKEYYDQLVKQNQSKNKIMKAKRNTIVLMKRYSQSPKKVSKLWKKVKNFDMLKCCSHYNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XD19</accession>
    <name>A0AAF0XD19_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0625229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XD19"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000005">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000024">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="108"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="770"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="205"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH05808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="801" mass="91252" checksum="F95E62771456E16A" modified="2024-05-29" version="1">MHKISKRSVSSLLRNGAVRHRSAAAPISAFNLSAGEVDTKPRWYSVVPTSGISVSSKLINIKSEFPLGIRYESTAAASESSDPPSEKYEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRFLSVTNSELIKDEVDLDIRIQADKDNGIITITDSGIGMTREELVDCLGTIAQSGTAKFLKALKESKDAGADSNLIGQFGVGFYSAFLVSDRVVVSTKSPKSDKQYVWEGEANSSSFTIREETDPVNLLPRGTRLTLHLKHDEKPFAHPERIQRLVKNYSQFVSFPIYTWQEKGFTKEVEVDEDPAEVKKDEQQDVNPEEKKKKTKTVVEKYWDWELTNDTQPIWLRNSKEVSTEEYNDFYKNTFNQYLEPLASSHFTTEGEVEFRSILYVPSVAPYEKDDIANPKTKNIRLYVKRVFISDDFDGELFPRYLSFVKGVVDSNDLPLNVSREILQESRIVRIMKRRLVRKTFEMIRRLSDSEKREDYEKFWDNFGKHIKLGCIEDRDNHKYLGPLLRFFSSQSEEEMISLDEYIQNMKPEQKNIYYIAAESVSSAKNTPFLEKLREKDLEVLFLVDPIDEVAITNLKSYSEKEFVDITKEDLDLGGEKDEVEEKKIKEEFGQTCDWIKKQLGDKVSKVQVSQRLSTSPCVLVSGKFGWSANMERLMKAQAVGNSSSLEFMRGRRIFEINPEHPIIRTLDNAYRNSPNDEEALRAIDLLYDTALVSSGFTPEKPAELGGKIYEMMDMALSGKWGSDRTRINEFQQEAATNPGTAEAEVIEPAEAEIVEPAEAGSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XDR9</accession>
    <name>A0AAF0XDR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Pachytene checkpoint protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0624646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a key role in chromosome recombination during meiosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AAA ATPase family. PCH2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XDR9"/>
    <dbReference type="KEGG" id="dcr:108224273"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19508">
        <property type="entry name" value="RecA-like_Pch2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000680">
        <property type="entry name" value="pachytene checkpoint protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058249">
        <property type="entry name" value="Pch2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23242">
        <property type="entry name" value="AAA_lid_TRIP13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23563">
        <property type="entry name" value="TRIP13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="198"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH05232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="467" mass="51936" checksum="0DA41D8C5A2D5F47" modified="2024-05-29" version="1">MSTPMEISEQQNPVNVTPSDLNGVVFEPPPPPALPSPAPTAISEDKFLVSVEVCLKSSSTARIEDVQTAVEKMLEKRSLSYIDGPIVIPPDDQFLVDNVQRICVCDTDVWVENHDILLFWQVKPVVHVFQLSEEGPCEDLSGDNNLSSFNEWMLPAKEFDGMWESLIYESGLKQRLLRYAASALLFTEKGVNPFLVAWNRIILLHGPPGTGKTSLCKALAQKLSIRFSSRYPQSQLIEVNAHSLFSKWFSESGKLVAKLFQKIQEMVEEENNLVFVLIDEVESLAAARKSALSGSEPSDSIRVVNALLTQMDKLKSSPNVIVLTTSNITAAIDIAFVDRADIKAYVGPPTLQARYEILRSCLEELLRTGILSYSQIQEGIGLQSLPNYCSLKEKLSVAVTQESQTPLHLCQQLYEVAEACEGLSGRSLRKLPFLTHAALANPSTCELYKFLCTMRDTIKRERSDLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH51</accession>
    <name>A0AAF0XH51_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF223 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0626313</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XH51"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Forms a channel through the cell membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin (TC 1.B.8.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNF2"/>
    <dbReference type="KEGG" id="dcr:108196910"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000003">
        <property type="entry name" value="Outer mitochondrial membrane protein porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF60">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN PORIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0626">Porin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH09661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="276" mass="29162" checksum="E5DEF7024C11B8BB" modified="2024-05-29" version="1">MGKGPGLYSDIGKKTRDLLYKDYQSDQKFSITTYSTTGAVIASSATKKGELLLADVSTQLKHGNITADIKVDTGSNLLGTITVDEAAPGLKTILSSKIPDQRSTKLEVQYLHDHAGISASVGLIPNPIINFSGAVGSSVASLGTDVSFDTKTGDFTKYNAGLSFTNADLIASLALNEKGNQLSASYYHLLSPLTNSAVGAEVTHNFSTNENTITVGTQHALDPLTTVKARVNNSGKVHALIQHEWRPKSLFTISGEVDTKAVDKTAKLGLALALKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNH2</accession>
    <name>A0AAF0XNH2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Pectinesterase</fullName>
            <ecNumber evidence="5 12">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0830794</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
            <location evidence="1 12">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="5 12"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNH2"/>
    <dbReference type="KEGG" id="dcr:108197632"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15799">
        <property type="entry name" value="PMEI-like_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018040">
        <property type="entry name" value="Pectinesterase_Tyr_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00800">
        <property type="entry name" value="PECTINESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6 12" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041770490" description="Pectinesterase" evidence="12">
        <location>
            <begin position="29"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="14">
        <location>
            <begin position="26"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH11313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        </source>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="17" key="1">
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
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        <reaction evidence="12">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
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        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
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            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
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    <comment type="similarity">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
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    </dbReference>
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        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="323" mass="35007" checksum="20B7BC1514C250E0" modified="2024-05-29" version="1">MADTSLYLLSLLFLPLSLLLILFFLVRPRHVSIPIKSRHVFITGGSSGIGLALAHRAAADGARVSILARNLEKLEAAQQSIRLATGIDVAIYSADVRDYDAVKGALEQAGDVDVLVCNQGVFVPQELETHDLEEFKFMIDVNVIGTFHLIKAALPGMKGRKDRGPGSIAIISSQAGQVGIYGYTAYSASKFALRGMAEALQQEVIAEDIHVSLIFPPDTETPGFVEENKRRPELTSIIAASSGSMKAEEVARKALNGIKSGKFIVPCNFEGVMLSIATAGLSPQPSFLMAFVEVVAAGILRFAALCFQWNWYSSIEGWHKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUM4</accession>
    <name>A0AAF0XUM4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0832244</name>
        <name evidence="9" type="ORF">DCAR_0832246</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUM4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF859">
        <property type="entry name" value="OS03G0690500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="213"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH12738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="367" mass="41324" checksum="70A87D89B15C6AC5" modified="2024-05-29" version="1">MEAPLSTTSSSNRQRELQAFDDTKAGVKGLVESGVQKIPSIFVHPPDAFDKNSNHKCKEFSIPVVDLGCLDKDPMSHQEIVDKVRCASETFGFFQVVNHGIPVTVLEEMLHGVHRFFEQDTEVKKQFYTRDLSRKVVYNSNFDLFTGRAANWRDSLSCSMAPSPPLEELPEPCRDIQIAFSEQVMKLGITLFGLLSKALGLDINHLEDMECAKGLELFCHCYPPCPQPELTLGTTKHTDFHFLTVLLQDQIGGLQILYQDHWIDVPPLPGTLIVNIGDLLQLLTNDRFKSVEHRVVAKKIGPRVSVACFFSTGLMPSPIPKLYGPIKQLVSEDNPPKYRETTAQEYLSYSYSKGLDGESRLLHFRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XX40</accession>
    <name>A0AAF0XX40_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0933973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XX40"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF56">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH14454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="148" mass="16280" checksum="DFA18CEB814EA68E" modified="2024-05-29" version="1">MNRTESSSPLDPEEEMKKDAVKYFGFQPKRDSASDARNALLVVAALVATVTFDKGTDVPGNIRNGTARIIFIIANSIAFSASMSIVEYLTTGFPFKTEIYISLSSVCFAYGSGVSNHIEGVREYVLLFISLLLPFFASKVSRVKAWLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XY36</accession>
    <name>A0AAF0XY36_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF57">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="2">
        <location>
            <begin position="219"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="943"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="1190"/>
            <end position="1293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH15402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1493" mass="171025" checksum="75B76816F5A6FB73" modified="2024-05-29" version="1">MCSSGGKVDHKINRGGAPFCFKIRGQNMHFIGSLIPEEGSKPKFCQLYIYDTDNENSNRIGAVGSNTDDVDMEIVEGLTRMLDENNKLVRYFRSARQRHKSGDQTEFKLILISSQAQNGRPNIIGPSNEVAGLIVNASADTAGCRDTVCQTKQGHLKRVFETDPFFMQLQFPLLFPLGEDGYHTRIPLRKTKKRSLQIEDAVDHEGERKIRDHVSMKEYYSSKLMIRLNEGLTPHLGGRLWQQYVIDAFTAMEQYRLDWISRNQTTIRSDLYTSVRDAVRRGDNDPSHVGKCVILPASFTGSKRYMSQYFKDSLALCRSIGHPSLFLTMTTNTKWPEIQEMMKNLPGVNVADAPDVVARVFKLKLDQLIELIKKKNFFGRCIGLMHVIEFQKRGLPHAHMLIWLDDRDKPKTAEQIDKLVSAEIPDEHEDSIGYNAVKNYMIHGPCGKDATYSACMVNGRCARHFPKRYSGVTCFDESGFPIYRRRNTGRTVLRKGHVLDNKFVVPYNRELLLRFQCHINLEICNNSRSLKYLFKYCLKGHDTATMLIKRKNDNSTKTASPASKSKVFDEVKHYLDGRYVCASEAAWRIFGFDIHSRWPSVDRLPIHLPESKYVSFKTGDSLQTVCNRADAKLSKLEAWFVANQKLPEARNYTYAEFPSYFTWLPRECKWKYRQRGEVVGRLSEVHPTAGDLLYLRMLLLRKKGCTKFEDLRTIDGVLYETYKEACGALGLLQNDKQWHDALSENAHSSFPHQIREMFVNILSYSSVADPHLLWKSHWKCMSEDILVKRRNATGNFELELTEADIQNYALAGDIQFHTCQNRVIREFSDWQLKVGDGKVDPISFKENVSEVLFRLPAQHVLHSDDKPIQKLIDVVYPDFANNCSSSEYFSRRAILTPTNVVVDDINYAILEKLPGETYTYLSQDSLEDQGVEENDFDESFPIEYLNSLNLPCIPKHELKVKIGTPVMLMRNLNQINGLCNGTRMIVTGCKKNTIECEIMSGSHAGKKHLIPRIEMIPTETPWPFDFKRTQFPIQICFAMTINKSQGQSMDVVGLFLPRPVFCHGQLYVAISRVTSAAGLHILVINDEGKTSDVTSNVVFPETYLRGGMHTICHNLPIMGQSLQHTNNSHQICKGKLFNGNTFAVTQRYMFLFTSRSSSLFKLIFTLIRHNNFSSWKIVQKLSVNMQGRMFDHIQSLEKSRTNWRIKARLTRFWPTFSPETSTIKGYNLILLDDDFPGASIFVYCDICLYTQNTHEVAEGKIYTVENFQVRDTIGKLRPVSTKLCIRLLSSTIIECVEDDALIPNHKFEIQNLLTVRKIHSITDSSSEPAPRAVIERTSFKELIENSLSFVLKRTVVVKFVINKIEEEDSWWFNSCVSCHSEVEKVDKKFKCAECKRNFGYCEKRYIQFRIVVLADDTTLVTNVILLDRVVKRMGATTVANILNLMKKVTSFPYQIYSNINQISPSNFRFQNSNSRILLSLLSLLCSSPLLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1I2</accession>
    <name>A0AAF0Y1I2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0936054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y1I2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558:SF3">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="2">
        <location>
            <begin position="246"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="497" mass="55580" checksum="E3787CA0FAC54C44" modified="2024-05-29" version="1">MCAMKICEKKLKIKETRIREKAARVCKFYIGDFLDDMWFDSAAILESDCSDDDFQSISDDVSSQHESEAASRLSISSLKDAHHRNGDANVPYPHHSAQLQKQEDYSAGHSARNSVSDTSGGSLHRGEQDSRLRSDGTSHDNSVFVDETSSVEGSGGRGDDLLDNCGIIPNNCLPCLASTVPTVEKRRSFSSSPPHAKKKAAIKLSFKWKEGNSSSSLLSSKMLIKRPIAGSQVPFCPADKKMPDSWSQIAASTFRVRGENYLRDKKKDLAPSHAAYNPFGVDVYLSQRKIDHIARFVGLPNISSYGELPPILVVNCQIPLYPAALFQSETDGEGINFVLYFKLSESYSKELPSHCKEGIKKLIDDEVEKVKGSSVPFRERLKILGRVVNVDDLQLSAAERKLMHAYNEKPVLSRPQHEFYLGENYLEIDLDMHRFGYISRKGFGAFLDRLKHCILDFGLTIQGNKPEELPEQILCCVRLNEIDYSNYQKLGLSQESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQ45</accession>
    <name>A0AAF1AQ45_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine gamma-lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207374</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQ45"/>
    <dbReference type="KEGG" id="dcr:108209382"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000087">
        <property type="entry name" value="Putative methionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF80">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="47439" checksum="C53B5F250618AFF8" modified="2024-05-29" version="1">MADSLYLSTNSKKRSADEFLAAKKQMKHWESDPAASLANARHEFGEHGGVNMSIEASATFTVMEPETMGRMFSGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTEAAYCTASGMSAISSVLLHLVSSGDNVVASQTLYGGTHALLTHFLPRCSGISTTFVDIQDLGMVDEAIVEGKTKVLYFESISNPTLTVANIPELSRLAHRKGVKVIVDNTFAPMCISPAKLGADVVVHSISKFISGGADIIAGAVCGSASLMNSMMDLHQGPLMLLGPTMNAKVAFELAERIPHLGLRMKEHSHRALVFATRMKKLGLRVIYPGLEDHPDHALLTSMINKEYGYGGLLCLDMETEEKANKLMNILQNCTQFGFMAVSLGYYETLMSCSGSSTSSELNDEEKKLAGISPGLIRMSIGYSGTLEQRWSQLEMAFSRLQDSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARH0</accession>
    <name>A0AAF1ARH0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0208848</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH02843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="150" mass="17234" checksum="6E71AD0B237866CC" modified="2024-05-29" version="1">MDPQEICKVAVLNRTFRGASYADFVCSIFQLASTVDATVNAEVFLWRTLWNHFFLRVFRAVLSILNDFVAFFTACNQKEEIKFFAHTKSDSRPLPGYAPTTEYNLVFTDQYQFLMLSQGSLDELNSQLEEPLPINHFRPRSNFVSGLCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3X6</accession>
    <name>A0AAF1B3X6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial</fullName>
            <ecNumber evidence="5">1.6.5.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Internal alternative NADH dehydrogenase NDA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Internal non-phosphorylating NAD(P)H dehydrogenase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">NADH:ubiquinone reductase (non-electrogenic) NDA1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0521675</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Alternative NADH-ubiquinone oxidoreductase which catalyzes the oxidation of mitochondrial NADH does not translocate protons across the inner mitochondrial membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="5"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3X6"/>
    <dbReference type="KEGG" id="dcr:108220811"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000009">
        <property type="entry name" value="Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="23">
        <location>
            <begin position="51"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="24">
        <location>
            <begin position="416"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="WOH02286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="486" mass="53444" checksum="AB7728F895F24C46" modified="2024-05-29" version="1">MHWLIKTLTRVSKSTTNKTKLDTLLFSRHFTSIATAETAELGPTKAGEKPRVVVLGSGWAGCRLMKDIDTSIYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVAEHIGRIQPAISTEPGSYLFLANCTALDTQKHQVHCQTVTDGLAKLDPRKFRISYDKLIIASGAEASTFGINGVKEHAIFLREVHDAQEIRRKLLLNLMMSDVPGVSDEEKRRLLHCVVVGGGPTGVEFSGELSDFIMRDVHQRYAHVKDYIHVTLIEANEILSSFDDRLRKYATKQLTKSGVRLVRGIVKDVQPQNIVLTDGTTVPYGLLVWSTGVGPSPFVQALDIPKAPGGRIGIDEWLRVPSAPDVFSIGDCCGYLESTGKPTLPALAQVAERQGLYLAKVLNTIGKAGGGYANAAQEMELGKPFVYKHLGSMATVGRYKALVDLRQSKEGKGVSMAGFVSWFIWRSAYFSRLVSWRNKLYVAVNWATTGAFGRDISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4N9</accession>
    <name>A0AAF1B4N9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0625488</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4N9"/>
    <dbReference type="KEGG" id="dcr:108227438"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000002">
        <property type="entry name" value="Soluble inorganic pyrophosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH06065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="24472" checksum="2913ACAD0DB7015D" modified="2024-05-29" version="1">MTPAIETQTKTANSKPSPHIPLNERILSSMTRRSVAAHPWHDLEIGPGAPVIFNAVIEISKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFVPRTLCEDNDPIDVLVIMQEPVLPGCFLRAKAIGVMPMIDQGETDDKIIAVCADDPEYKDYNDIKDLPPHRLAEIRRFFEDYKKNENKKVAVDDFLPATKAYEAIQHSMNLYADYVVESLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAM3</accession>
    <name>A0AAF1BAM3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0729488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAM3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000083">
        <property type="entry name" value="BEL1-like homeodomain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="373"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="375"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH10027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="551" mass="60125" checksum="6A9536257C282A91" modified="2024-05-29" version="1">MSTNNMNASSEAAMQLFLMNPSSARSHSPSHSMANSTLHMLLPNSASSVHQQVFQGPPAHFGPSSQQFGSHVVSNDISHLGGHVVDHGHGQGQGHGQGLSLSLSSSLQHLEAAKAEEFRMGDGSGMIYFNQVGSGGGSNASNVQYPYKNNMNSLGVGHNQGMHLQGEVVMGQNQFGFGSNSALGVVNLLRGSKYLKAAQELLEEFCSVGRGQFKTNKQLGRQNSINPNSSSTSGTAANGSAASSSKDPPPLSASDRIDHQRRKVKLLSMLDEVERRYNHYCEQMQMVVNSFDMVLGYGSAVPYTTLAQKAMSRHFRCLKDAIGAQIKKSCELLGERDAGGTSGVTKGETPRLKLLEQSLRQQRAFHQMGIMEQEAWRPQRGLPERSVNILRAWLFEHFLHPYPSDADKQLLARQTGLSRNQVSNWFINARVRLWKPMVEDMYQQEETEEREAFHAQTPTPSTTTPQDFAATLTAPAASRSEIMRNADNDFSQLAINKQQQQRFSKNQATYSAEVSQSFAGAATASGVTGDVSLTLGLRHAGNMPENNSALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BB84</accession>
    <name>A0AAF1BB84_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0730157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BB84"/>
    <dbReference type="KEGG" id="dcr:108194136"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF45">
        <property type="entry name" value="PQ-LOOP REPEAT FAMILY PROTEIN _ TRANSMEMBRANE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH10687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="395" mass="43685" checksum="9500D17170BFB6E9" modified="2024-05-29" version="1">MKNPLLSVVLKNSDVASSTSYCVKERRPCVGWVDKYFKDCLCDTRDKLSFASGMLSLISWGVAEIPQIITNFRSKSSHGVSLLFLLTWVLGDIFNLIGCLLEPVTLPTQYYTALLYTTSTVILVLQCLYYDHVYSWWKSKKVDANQPIEDVKKPLKPAKENDDDDSAIPATDEGKINSNFYFTSARSLAGSATPPNRSYLWTARSGPSALTLDGDSSSDDEVDAAVMTNKKSKTQPKPIPRSAGYGAFLATSLNLPRSTKALLQASVAFSGRKLLQGNADTEKFVVGQCLGWMMAAVYMGGRIPQIYLNIKRGSVEGLNPLMFIFALIANVAYVGSILIRSTEWDKIKANMPWLLDAVVCVGLDLFIILQYIYYRYLGKRTAGGRGQDDDYSESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BBS3</accession>
    <name>A0AAF1BBS3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase</fullName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0830768</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BBS3"/>
    <dbReference type="KEGG" id="dcr:108199726"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="7">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="8">
        <location>
            <begin position="348"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="545"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH11287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="639" mass="72220" checksum="B1356A39DD31CD12" modified="2024-05-29" version="1">MMKNRASSSGGSGNGNNRGESEVFEVGRRRSSCGYCKSSVRTSITYGLWANSLTVDDYQDLLDRGWRRSGCYLYRPEMDKTCCPQYTIRLKASDFVPSKEQQRVSKQMQRFLDGNFEVKESQKSLDNPYTSNNLSSLVQSSTIASAGQESLKPNTEEKNQDVELLSFLSDQINCAARACIERGEFPLDILLPKPSVKKVSQGKKKLLAEGSEDLLYSSNISFLIKAALVRRDRRHDMKGSGGSKQENEELINTELTSQAIAEKLVTYLNNLADTSGLSARACNGHINFYATTKQARSDEVVDVAMVRKESRGESVDEGSSMKKVSGGTLGKRKRLEIILKRSCFDPKEYELYRKYQINVHNDAPDHVSESSYMKFLVDSPLVYVPSNGDDTVPQCGFGSFHQQYVIDGRLVAVGVIDILPKCLSSKYLFWDPEYASLSLGKYSALQEISCVRENQAHCRSLQYYYMGYYIHSCNKMRYKAAYRPSELLCPLRYLWFPFEIAKPWLDKKRYVILSDLHNLQAVESSTPKLSSSQLEPPHDNSFQEENNDVPFDEDEEMSEVDSEDSEDESDPETNDLALPEFEDPNVGDVLIGLKGSRLRYQDIHHALAPNQRSHLVTKLRMYKEAVGEKLSKQMVLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDR1</accession>
    <name>A0AAF1BDR1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0933760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDR1"/>
    <dbReference type="KEGG" id="dcr:108202290"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="8"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="184"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH14243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="361" mass="39851" checksum="6E013F2EE1FE3AB5" modified="2024-05-29" version="1">MGDSLPFLVMVLLQAGYAGMNILSKLAMNSGMNPLIHVAYRQIFAAVVLVPFAYFLERKTRPRMTLPIFLQIFLSSIFGLTLNQLTYFVGLSHSTPTIACALTNLLPAVTFLMAVPCRLESVAIKSRSGQAKVVGTIVCVGGAMLLSFYHGSQVPIGESKIHWNYIIRNSRNSTNQTNHHNFMLGPFLLIASPVCWAVWFILQAKMSSTYPAPYSSSALMCLMASFQCVIIGLVSDHHISSWSLNPPIRALSSIYAGVVCNALSYCLMSWCIKRKGPLYVSVFSPFLLVIVAILSWALLEEKLYVGTVAGSGLIVTGLYTVLWGKKEEMISLANDFKIKHVEEFDIAKLENGDIEFVKCEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BE34</accession>
    <name>A0AAF1BE34_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0833183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOH13672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46993">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46993:SF6">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="321"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="323"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43318" checksum="AAF6BFDC7A965D01" modified="2024-05-29" version="1">MDPDIARWVLDFLVRQPLDDSTLTSLLTTLPLSNTDTHLTKLLLLKRIDSEISKNAVSESVLGLLEQMEELDYREHVEISDSMKRAYCIVAVDCTVRFLEEGEKGFYFEAVKRIWRTRIGCMMNFERVGLVSEELERWKDEIESAVWDDGACKVLLEKWGGLDVVESVRVYIEEARGRIGPSFLELVTESLSGEAVKEVFGIDKKDIHKEKVQRKSKPSAVKQHKKFSSKTTRGVKIVDNDDLTLEQQESHNKDSSLSPTDVAKFQEGLRTSTSSLGKKAEDPFPDTVNISKNTGNLGRLHLPSPKERVVSPLKKYESPKPLRRRRRLWSNVEEDTLRTGVRKYGVGNWKLILNMYRDIFDDRTEVDLKDKWRNLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BG81</accession>
    <name>A0AAF1BG81_DAUCS</name>
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        <recommendedName>
            <fullName evidence="10">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0935195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BG81"/>
    <dbReference type="KEGG" id="dcr:108202666"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000177">
        <property type="entry name" value="Importin beta-like SAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="887"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="939"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="892"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="945"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BKQ0</accession>
    <name>A0AAF1BKQ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
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    <reference evidence="3" key="2">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
        </source>
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    </dbReference>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
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        <property type="entry name" value="UBP1-associated proteins 1C"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039999">
        <property type="entry name" value="LYAR"/>
    </dbReference>
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        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058719">
        <property type="entry name" value="WHD_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014898">
        <property type="entry name" value="Znf_C2H2_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25879">
        <property type="entry name" value="WHD_LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51804">
        <property type="entry name" value="ZF_C2HC_LYAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="9">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KZM87598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH08950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="34800" checksum="44D8FE73D9623417" modified="2016-07-06" version="1">MVWFQCEDCGDNLKKPKLLGHFRMCSANKLSCIDCGQVFGQQSVQGHTQCITEMEKYGPKGQVKASTNTTPNPKSDSKMKPEVDINVGLSERPPWFCRLCNTSATSRQTLLLHAEGKKHRAKARAFHASMQPPKQTEELTPTTKVSSESNLKDRVVGNQDVVEPKELGLPEVAPAHNGSEMGTVHANSLTSKKRKHSSDKQNVEVGNGEDEIECKEKKSKQSVGKDDQVTGTKSFTREAKKNIKWKKLIASALESNSDGVLKLRKLQKFVQKALVDSEIMLDESQVKETVEQKINSSSRFSVDGKYVRLVVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UH60</accession>
    <name>A0A164UH60_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0729816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UH60"/>
    <dbReference type="Gramene" id="KZM88881">
        <property type="protein sequence ID" value="KZM88881"/>
        <property type="gene ID" value="DCAR_025956"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196674"/>
    <dbReference type="OMA" id="MHANEYS"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carb_kinase_PfkB-like"/>
    </dbReference>
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        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
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        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320:SF1">
        <property type="entry name" value="OS01G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="67"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH10349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="38540" checksum="6F1C8C592E619143" modified="2016-07-06" version="1">MGAETTAAFVAVEAPVIVGLQPSALVDHMARVDWSVLHIPGETGGSLPVAIEELEYILSQVKIHLQSSNGQSPLKTIAGGSVANTIRGLASGFGITCGIIGACGNDEQGELFLQNMKYNNVNLSNLIAKKGATAQCVCLVDGTGNRTMRPCLSSAVRLQADDLRGDHFRGSKWLVLRYGIFNLEVIHAAVRIAKQEQVLVSLDLASFEMVRNFRQPLLELLESGNVDLCFANEDEAKEVVRGEGEGHTDPEAALDYLGTRCKWAVVTLGSKGCIAKHGNQVVRVPALGETQVSDATGAGDLFASGFLYGLLKGLSLEDCCKVGACSGGSVIRSLGGEVSQENRHWMYKQMQINGLSVPELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UL96</accession>
    <name>A0A164UL96_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_026103</name>
        <name evidence="3" type="ORF">DCAR_0729982</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UL96"/>
    <dbReference type="Gramene" id="KZM89028">
        <property type="protein sequence ID" value="KZM89028"/>
        <property type="gene ID" value="DCAR_026103"/>
    </dbReference>
    <dbReference type="OMA" id="DKCQRKN"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33386">
        <property type="entry name" value="OS02G0740600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33386:SF5">
        <property type="entry name" value="OS02G0740600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM89028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="75" mass="8334" checksum="882DC9B26D365293" modified="2016-07-06" version="1">MDLGNNYNNSYGTSWADQWDPLPEYSQKTSQKSGTSAVASKGLEKTKTVASTGAKKVKGGFMWIKDKYQKTTHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VVE7</accession>
    <name>A0A164VVE7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pescadillo homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_021719</name>
        <name evidence="7" type="ORF">DCAR_0624494</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pescadillo family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VVE7"/>
    <dbReference type="STRING" id="79200.A0A164VVE7"/>
    <dbReference type="Gramene" id="KZM90916">
        <property type="protein sequence ID" value="KZM90916"/>
        <property type="gene ID" value="DCAR_021719"/>
    </dbReference>
    <dbReference type="OMA" id="QKVTWIV"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000002">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="339"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM90916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH05082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="595" mass="68720" checksum="A2E8845700F8D50D" modified="2016-07-06" version="1">MPKHYRPAGKKKEGNAAKYMTRSQAIKYLQVSLSLFRKLCILKGVFPRVPKKKVKGNHHTYYHVKDIAFLKHEPLVEKFRDMRTYDRKVKKAESKKNRDLAERLLTRKPTYTLDRLIRERYPKFIDALRDLDDCLSMVHLFAALPAIEREKIEVKRVHNCRRLSHEWQAYISRCHKLRKVFISVKGIYYQAEVEGQKVTWLTPHALQQVLPDDVDYKVMLTFLEFYETLLAFVNFKIYNSINVKYPPILDPRLEALAADLYALSRYFDANSKAPMVSSQDAALSESNQVKDKNNDAELHESELRLAQLQHQLPSNEPGALMRLVEDAAGEDEDDEETKECKTLFKDMKFFLSREVPRESLLFVIPAFGGVVSWEGEGSPYKETDQGITYQIVDRPTQGHKFFTREYVQPQWVFDCINARIILPTEDYMVGRVPPPHLSPFVVYDPEDHVPEYAETIKRLQAAARQEVLPMPGVVGVEDLENPQNLLDEAIIDRTEAIELLKLQKQHLDELNKEVKGEQLAVESDASDKKPVDTGFIAADSEQAIKDAADITMVGVSRKKRELYKAMQIGNERKKAKTQLLKERKRLLDEAKKSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WE05</accession>
    <name>A0A164WE05_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WE05"/>
    <dbReference type="Gramene" id="KZM91651">
        <property type="protein sequence ID" value="KZM91651"/>
        <property type="gene ID" value="DCAR_020984"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224327"/>
    <dbReference type="OMA" id="CKKQLNR"/>
    <dbReference type="OrthoDB" id="429961at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF7">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="508" mass="57577" checksum="AFFBED4111209987" modified="2016-07-06" version="1">MGTTISTIKPSCNTNNLIKTKSLLSLSRYSTATQSKPSNSSRLFQRINPLRDPKVSILPVLDQWITEGNKFKEPDFQRFVRELRASKRYSHALQLCEWVNINNFYPVSSGHLALQLDLIGVARGLDAAECFFDKLSEKEKDERTYGALLNCYVREGLVDKSLLHMQKMKEIGYASSPLVYNNLMCLYSRTGQLEKIPDVLTQMKENGISPNNFSYRICINSYGERSDFDSMQKTLEEMECQPHVTMDWTTYSTAVNHYIRAEQKEKALVVLKKLEAKLNKDALGYNHLISHYANLGNKNEVMRLWGLQKVVCKKQVNRDYITMLGMLGKLGEIEESEIVLKEWASSCQTFDFRVPNVFLIGLCSKGLVEKAETTLKNIINTGKTPIPNSWAIISLGYKEVENMEKAFECMKEALAVAEGSPGWRPKPALISSILHWLGEKGEIAEVEAFVRSLRSVIAVNKEMYHALIKANVRGGIGVDEIIESMQFDNIDIDEGTEEILGLRKEVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WT30</accession>
    <name>A0A164WT30_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AIPP2-like SPOC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_020427</name>
        <name evidence="9" type="ORF">DCAR_0625974</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="79200.A0A164WT30"/>
    <dbReference type="Gramene" id="KZM92208">
        <property type="protein sequence ID" value="KZM92208"/>
        <property type="gene ID" value="DCAR_020427"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227421"/>
    <dbReference type="OMA" id="AGRDNME"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140566">
        <property type="term" value="F:histone reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056280">
        <property type="entry name" value="AIPP2-like_SPOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049914">
        <property type="entry name" value="PHD1-3/5-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33304">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33304:SF9">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23121">
        <property type="entry name" value="SPOC_AIPP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AIPP2-like SPOC-like" evidence="7">
        <location>
            <begin position="970"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="700"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1114"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1201"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="673"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1122"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM92208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH06546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1581" mass="173704" checksum="3BC59BEE215F54FD" modified="2016-07-06" version="1">MVKRKERIVTDLYSGTPNPQGVTSQPNKGIHRHTLDQNINVKVDPGTCNVCFAPCSSCLHTNRTRMEPKNAEYLDETSRESAISYSVDDTVETKKSRGRKSSQHTASEEGNMITMNSNLGLCSENAEMETALELTNISGSFQDTEVPNKLLYGGPVKEKLHTPSPPKKDLDDGNSMSNLVDMRGLEGHDDNISCVSGSDEASNMSNSNKEITDTKALTFAGSPSSLASEDYIKAGQSQEASFMDKHKLEVQNKSSGEVLSRAVSGQKSALYASCDIQENIIGHVGGNSEPSAIECLKVEAEYNRVEFPSKLLISLEEKKEVEKDSELLALPEARETSMHSDPVNESDESDILEHDIKVCDICGDAGREELLAVCCSCSEGAEHTYCMKEMMTKLPEGDWLCEDCKFEKREREEKAKYNLVDGDGVAQKNADLGNLDGLHTDANIIKAEKESLHGKIYRKRKADDTEVSSSAKRQVFESTIRSPKISSPRRADVLSRDSSFKNSDRGKVKPASQIPPGVQTGKSIPEVANPSLDARQSTSRGTFLRSKSFSSASAKQKVKHIDEVVPQKHKSNRDNASLGTKGGGSRTMGKSMSFHAFNSGGSNSTDLKAKMFSPKYSHGQDIKGQNTKERSLVERKNSLRLERPLVNSVVTTSTTLSPEVDRNLPVRDKTGPFCSTSNNRDVKSAQSDNKLIQVSRPVNKAISNGSDVPVLSGEGTKQLSASRNDNDEGKTVNAKTKVDCSSTSLSIEGPISKSSESLPDGLNKTRVSTNMVESARGNSNTQLKHSTAGEKFTLCQKCKEVGHSAEFCTIDSPEKSLVPDLHTSRSSKDLIHKDNKLKAAIEAALLKKPGLYRKKRTTDHFDELTVSGINSEVCSQDQQTNTRILRKSVSGDEVSMEAASAWNTNTEFVKQATGADVKQLTNSAEAVTALLHWPLPPATVKSVMDLPSNSHMSISDLPITLAIPKHEYIWQGCFEVCRSGKQPGCYDGFQAHLSAGASLKVLETINKFPSTVVLNELPRHSVWPIQFEENGVKEEHIALYFFARDHESYEKSYKSILESMIRNDLALKGNIDGVEILIFPSNQLPVKYQRWNAMFFFWGVLKGKRNSCLQKVPVSPKKTAGSRDTGTANMSSSLVPVDKDSPTFKKAGKTISDVHPMIDLQCSPLTKKNNGNIDGKSVSHSQQSDCVNATKEQQGCRLDCNSVPTDEIKPPESWEDTGSRTNSLERQVDKDSAIKVARSACDSNSSDDKETGHLSTPYDLHHLPQINSKVAAGEQDPASSRILGEVDNEEKLRMGNSGTEGFFEAETVAEKVTPKLPSHKEPYSRPSTCTKQLNIDSLVSKSEASIFGSSQALHVNDRDSIFDTGGIVNKKPRLDYNDLYDLNDHTSSSRDSLLMQDSVTSFPVLKKHEEGSDETSVRTLENAERYFFPVDPYHAQHLGLGDSSVLQKRRLSENVEEFFQDKIPNLELALGAEMKLPVKQSLPHFLASEIEKNNQSQPPGKLPVKQSFPHFLAAQIEKNSQDQPPGRTFTTAEEDASAALSLSLAFPFTDKAQAGRPVETKELLPTERQHEVNFLKGFLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WUE1</accession>
    <name>A0A164WUE1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0626036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WUE1"/>
    <dbReference type="Gramene" id="KZM92263">
        <property type="protein sequence ID" value="KZM92263"/>
        <property type="gene ID" value="DCAR_020372"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228041"/>
    <dbReference type="OMA" id="TVRMSWD"/>
    <dbReference type="OrthoDB" id="10253151at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.100:FF:000006">
        <property type="entry name" value="Ribosome-binding factor PSRP1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.50:FF:000003">
        <property type="entry name" value="ribosome-binding factor PSRP1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="entry name" value="Ribosome hibernation promotion factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="entry name" value="Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050574">
        <property type="entry name" value="HPF/YfiA_ribosome-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33231">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33231:SF1">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="Ribosome binding protein Y (YfiA homologue)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH06608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="33802" checksum="29F522476CB3EE3E" modified="2016-07-06" version="1">MATLFPTIKTTVNHRPPPPSCCLATKNVAHSKITLPSPSTSIFRFYRNPLSGKIFPVTKLRRSGGTRMAWDGPLSSVKLILQGKHFELSEDVKGHVEDKVGKAIQKHSHLVREVDVRLSVRGGEFGKGPKVRRSEVTLFTKKHGVIRAEEVAETMYASIDAVSSVIQRKLRKIKEKDSDHGRHMKGFDRLKVRDPEAMLLQNGSISSAQVEDEDDEDPFMDEIVRTKYFDMPPLTVAEAIEQLENVDHDFYGFRNEDTGEVNIIYRRRAGGYGLIIPKQDGKAEKVEPLVVEPARKSSVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WWP2</accession>
    <name>A0A164WWP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">beta-carotene 3-hydroxylase</fullName>
            <ecNumber evidence="5">1.14.15.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BCH-1</name>
        <name evidence="8" type="ORF">DCAR_020269</name>
        <name evidence="9" type="ORF">DCAR_0626164</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.24" evidence="5"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017254252.1">
        <property type="nucleotide sequence ID" value="XM_017398763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WWP2"/>
    <dbReference type="STRING" id="79200.A0A164WWP2"/>
    <dbReference type="GeneID" id="108224246"/>
    <dbReference type="Gramene" id="KZM92366">
        <property type="protein sequence ID" value="KZM92366"/>
        <property type="gene ID" value="DCAR_020269"/>
    </dbReference>
    <dbReference type="OMA" id="FRIDEMR"/>
    <dbReference type="OrthoDB" id="9990796at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010291">
        <property type="term" value="F:beta-carotene 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016119">
        <property type="term" value="P:carotene metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016123">
        <property type="term" value="P:xanthophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045019">
        <property type="entry name" value="BETA-OHASE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31899">
        <property type="entry name" value="BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31899:SF9">
        <property type="entry name" value="BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="149"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM92366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH06736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="309" mass="34256" checksum="B388910C295BC234" modified="2016-07-06" version="1">MAAGISAASSSTSFSLGRNPFLGPNPIWLFAPSVRKLNPSLRFKQKSLTTVCFVVEPRNDSSGKPENNADRDEVSREEIEAGSCSVRVEERRARKKSERFTYLVAAVMSSLGITSMAVLAVYYRFSWQMEGGEIPYSEMIGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGAFELNDVFAIMNAVPAIALLAYGFFHKGYFPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVEDVGGHEALELEINRRIKSSASRASRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164Y0A8</accession>
    <name>A0A164Y0A8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0519502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164Y0A8"/>
    <dbReference type="Gramene" id="KZM93801">
        <property type="protein sequence ID" value="KZM93801"/>
        <property type="gene ID" value="DCAR_017046"/>
    </dbReference>
    <dbReference type="OMA" id="EMRELRY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1185">
        <property type="entry name" value="DISEASE RESISTANCE RPP8-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="166"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="429"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="582"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH00144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="916" mass="104562" checksum="6CC2F85E3FDF4F5B" modified="2016-07-06" version="1">MAETVVSFAVERLGELLISEIKHLHGVSDKVKEIQRELKRMQCFLKEADKKQNQDERVKNWVAEMRELAFRIEDVIETFAIQVPTKTQKSGFKKMLRRFACMLSEGVSRHNISSEIDAINDEMANLRTSLPSYGITKGLQEGETSNLTSKRIFYSHIVEKDFVGMEKELKQLICSLKRDDKGSEVVSICGMGGQGKTTLAQKLYNHVEVKAHFQKLAWVCVTQQFDREKILKEALKQLISDTRKQEVINMDNGELVRELYQVQKECNCLVVLDDIWAMDSWRLLRDAFPVGESTSGSKVLLTTRNETVAELGLVHRIQDLTTEEGWQLLSKKAGISDILPASRMEILGKSMVEKCKHLPLAITSIGGILNGKLLRDWETINKDISFYLLQGEGSVSKDDRYYTVRQVLGLSYDSLPPHLRHCFLCFANFNEDEVIKTQKLYTLWMAEGLISVEHKSERKMLLDIAESFMDELAHRSLVQVKKRENEDKSWSKYKTCSVHDLIRDFCLSKAMEENFIKVVHSQQPLSKDELRASIARRLCVNSYDESMLKLCDQDMISHVRSLFIVKGIPGCSKGFFLWPDKVLSLQKFKLLRILTVKQFELSHHDMKMISELVYLKCLSLHGCKVEELPFSLSKLRNLEILDLAGSQVGKMANVLCKLKRLKYLYLPQMFQVEKLRFEGLDELEVIHKFDSDYCDISDLIGLKKLKVLRAVLCTEKDTVSGKSVLDYIESRELRQSEIFIGSKDEFCLKSWLECIFINVLVMYGPICRFPNPTSLSSSCLSYLMLSGCGMEEDPMMFLEKLPNLRKLGLLRDAYLGSEMACTAKGFAKLQVLYMYSLSGLKQCRVEQGAMPNLSFLEIDCCVELEMLPEGLSCLAALESLRIRNMPGAFIERINGIGGAEGVDMYKVSQVSNIEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A3L1</accession>
    <name>A0A165A3L1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_015780</name>
        <name evidence="4" type="ORF">DCAR_0414922</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM96858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_015780"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218277"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
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        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="23"/>
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        <location>
            <begin position="24"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="48"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM96858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="33679" checksum="616BCC69245A0F70" modified="2016-07-06" version="1">MVNIFAILKPVVWLLMKFYGLSPQIVETEPGTKIKIWVPSKTKSANKPNVVFLHGFAGDGIFSWMSQVMAISSTHAVYVPDLLFFGGSYTDKPGRSTAFQAEVMAAALRKVGVKKCTVVGLSYGACVGFKMAKLDPELVEFIVASDTIIELNESFVRVVLDHYGFDNFVEFLMPKTADGLIRFLTICNHGPVNLPQFAAKDFLREFYNNRKEKAELAEAWVIPDNEVKPISLSQKVHLVWGDDDKIFNSNVAERTKMQLGGHTTLHYIEDGGHAVQMEKPDLYNAELKKVLSSRIINSRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WUI2</accession>
    <name>A0A165WUI2_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0415863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WUI2"/>
    <dbReference type="Gramene" id="KZM97677">
        <property type="protein sequence ID" value="KZM97677"/>
        <property type="gene ID" value="DCAR_014961"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215614"/>
    <dbReference type="OMA" id="EDRCDEE"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR053063">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851">
        <property type="entry name" value="ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42851:SF19">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="707"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG96527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="846" mass="93077" checksum="35D4C0E85A1CC4CD" modified="2016-07-06" version="1">MSVKFGDIDLNSDLLPIEENKDFDENRTARMVNSLADDTLINQSSNQIRVDGARVCGVEEIRVSGSGKAVDSEVGGVLKSGGTETSDGGEQLNGMVSEDLNGVAGEGGTEVRTDLGVKNNGVAAVHSSVDMEDASFQGGNNVLSLVVNLCTDDAAFGETKFNSMEGERVNEMNNLSENPSIVLDGLVNNAATQLNSIEIENPGVTSEEKEACIKEVANSNKGCQKNDVIKEDLHIEKEGDYHVSDLVWGKVTSHPWWPGQIYDPSCASKKALKYKKKDSFLIAYFGDQSFAWNDASRIKPFRMYFSQLEKQSNMESYTHAVSCALEEVSRRVEYELTCSCLPKELVSKIESQEVVNAGIRKKKGVTVGGDIFSTAASFAPDGLVQYLKEIAATPLDTINSLEFVTARAQLLAFHRWKGKGHAQRPSFTMLGGLFESDDFLISEKERDLSKLTDYALAATNVDGEGKSVVEDSSFSKQKHITRDGLRPRKKKCMVDLMPEDYLDDEDDVEEEVVEKYIYPAGKESLMVESTRPTISRGNSGKIHVSPAEKLFEVGDRIRKVASQLSGSVSFLRSGESTSQHVKIEGLNHCRSSGVSFSGLEKVPKADMGNEYPVAEMLFHLSLVAGSPMEVGSNKLISVIRFFTDFRNSVCLDPENSQLLTSELSTNCNPKNSIDGFNGVEASYQTNMISQKISREHILNEVETEKEMTNVEEAAGQSNVNPSDANEEPEKDSPTAFILNFSDLESIPSEENLNKIFSRHGPLIESDTEILKKSNRAKVVFKRRDDAESAFSSPGKFSIFGASLIRYQLNYSPSPRKPSNLGRKRKRKDVTSDNDKGKGHQVMIESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WVG3</accession>
    <name>A0A165WVG3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_014932</name>
        <name evidence="3" type="ORF">DCAR_0415893</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WVG3"/>
    <dbReference type="Gramene" id="KZM97706">
        <property type="protein sequence ID" value="KZM97706"/>
        <property type="gene ID" value="DCAR_014932"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM97706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG96557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="170" mass="20149" checksum="C968E955093FB774" modified="2016-07-06" version="1">MDHDLPPRKRHSYRIKPLSPSPPPPPVAFFRRRRMAYLTYRTLPLIKEDGKLSKWKIFYIRPQNQGGYQQLRETLYNKAGANAEGVSHDHQAVIYYLTRQDLVLNRDQPFKRLDGVLDWNTFANSVQRFRIVWVPLNKNSSNRPAEPDRNKKYSSDRSARYSNARQFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WWL1</accession>
    <name>A0A165WWL1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0415924</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WWL1"/>
    <dbReference type="Gramene" id="KZM97729">
        <property type="protein sequence ID" value="KZM97729"/>
        <property type="gene ID" value="DCAR_014909"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217725"/>
    <dbReference type="OMA" id="NALPCAR"/>
    <dbReference type="OrthoDB" id="5210at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG96588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="759" mass="79976" checksum="1FB7EE2F14DDE635" modified="2016-07-06" version="1">MGNELLSEMGTEIIIPVCAVIGIAFSLLQWLLVSFVKLSPDKSSNGKKEPLIEAEEGGNDHSVIIRCADIQNAISEGATSFLFTEYQYVGVFMVAFAILIFLFLGSVEGFSTKSRPCTYDASRMCKPALATAVFSTISFLLGVVTSVVSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAANGLLVLYITINLFKLYYGDDWEGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGISHDFTAMCYPLLISSMGILVCLITTLFATDFFEVRNVKEIQPTLKNQLIISTVMMTFGIAVVSWIALPVTFTIFIFGQQKEVQNWQLFLCVCVGLLAGLLIGFVTEYYTSNAYSPVQDVADACRTGAATNVIFGLALGYKSAIIPIFAIAFSIFVSFSFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAEIEVVDVLTPKVFIGLLVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIREMIPPGALVMLTPLVVGIFFGVETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGVSEHARTLGPKGSDAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZJB3</accession>
    <name>A0A165ZJB3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0310010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZJB3"/>
    <dbReference type="Gramene" id="KZN00102">
        <property type="protein sequence ID" value="KZN00102"/>
        <property type="gene ID" value="DCAR_008856"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210785"/>
    <dbReference type="OMA" id="YMGRNFS"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF64">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="63"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="353" mass="37791" checksum="8B9ED2723DF8901D" modified="2016-07-06" version="1">MESDIQTKFTYLGRTFNSLNIGDSSAAFSDCNSDRSGEFSTTSTQSRRLLTATLAGNSDDYVIEQLVSDLQSSSVDDQKMAAMELRLLAKNKPENRIKIAQAGAIKPLISLISSIDPQLQEYGVTAILNLSLCDENKELIASAGAIRPLVKALRIGNSTAKENAACALLRLSQIEENKVAIGRSGAIQLLVSLLETGNLRGKKDASTALYSLCSVKENKIRAVEAGIMKPLVELMADFGSNMVDKSGFVLSLLVSVAEARVAVVEEGGIPVLVEIVEVGSQRQKEIAAAILLKLCEDSSVYRTMVAREGAIPPLIALSQSGTSRAKEKAETLVELLRRPRSGNTAARTSDVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AJJ0</accession>
    <name>A0A166AJJ0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0101220</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRP receptor beta subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AJJ0"/>
    <dbReference type="Gramene" id="KZN01358">
        <property type="protein sequence ID" value="KZN01358"/>
        <property type="gene ID" value="DCAR_010112"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215189"/>
    <dbReference type="OMA" id="MNGVKVT"/>
    <dbReference type="OrthoDB" id="41266at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04105">
        <property type="entry name" value="SR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG82060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="31778" checksum="A54AB26ECEB048B6" modified="2016-07-06" version="1">MDMNEKIEILKSEFQKLSKDGQAWVQHLSVEMNKQEWFQKLPPFIQNIPPLQLYVALVVLFLTMFFFFIVRLFNRKTSNTILLTGLSGSGKTVLFYQLRDGSAHQGTVTSMEPNEGSFVLHSETTKKGKVKPVHLVDVPGHSRLQTKLDDFLPQAAGVVFVVDAVEFLPKSRAVSEYLYDILTKASVVKKNIPVLILCNKVDKVTAHTKEFIRKQLEKEIDKLRASRNVVSEADVSNEFTLGIPGETFAFSHCRNKVTVAEASGGTGEITQLELFIREHVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B4K9</accession>
    <name>A0A166B4K9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_011105</name>
        <name evidence="5" type="ORF">DCAR_0934666</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B4K9"/>
    <dbReference type="Gramene" id="KZN02351">
        <property type="protein sequence ID" value="KZN02351"/>
        <property type="gene ID" value="DCAR_011105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="176"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZN02351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH15129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="377" mass="42584" checksum="38CDEE9552CA79B2" modified="2016-07-06" version="1">MSAWTKKKLNQAWKQCKKKLTSGFVSGQPSEFVSGQQRCSTCNSVIYEERLARLEHAVFSKKQCHDDENIFDIYGHGEEDKADEMDGHGEEDKLDENLPLMEVLSTLKSKRKRTKKSPQPKPSPSLKRKNKFGTTYARRKKTSTISSSTGAPPTIVSDVISEKTSPCQITGPDRAIVTRRKLLVLDINGVLACVLHGMPHNGFQNYIPIYIPSDSETQTEACMKSVIKRPQCEEFLKFCLERFDVGIWSAKMEKTVTPSINATISFSTRIELKFIWDQSNCTRSKEGELCKETRKLREIGYDDVANILLVDDSKHKAKLNLPNTAIFPTSYNYKNLNDDELGPNGNLRLYLKGLAAATTTSVSDYVRDHPFCPSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B7X4</accession>
    <name>A0A166B7X4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0312696</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B7X4"/>
    <dbReference type="Gramene" id="KZN02476">
        <property type="protein sequence ID" value="KZN02476"/>
        <property type="gene ID" value="DCAR_011230"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212594"/>
    <dbReference type="OMA" id="HCEPNYS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000012">
        <property type="entry name" value="protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000131">
        <property type="entry name" value="Protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF18">
        <property type="entry name" value="PROTEASE DO-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="465" mass="51338" checksum="7FBE6F140794DCB2" modified="2016-07-06" version="1">MSSMDAVVKVFCVHTEPNFSLPWQRKRQYSSSSSGFIIEGRRVLTNAHSVEHHTQVKLKKRGSDTKYLATVLAIGTECDIALLTVSDDEFWEGVSPIEFGELPVLQDAVTVVGYPIGGDTISVTSGVVSRIEILSYAHGSTELLGLQIDAAINAGNSGGPAFSDKDKCVGIAFQSLKHEDAENIGYVIPTPVILHFIQDYEENGNYTGFPVLGVEWQKMENPDLRMSMGMGLKQKGVRIRRIEPTSPESLLLKPSDVILSFDGVDIANDGTVLFRDGERIGFSYLVSQKYTGDKALVKVLRNSKTLEFNITLATHTRLIPAHIKGKPPSYYIVAGFVFSAVSVPYLCSEYEKDYDAPVKLLEKHLHGMAQSVDEQLVVLSQVLVADINIGYEYIINTQVHAFNGKPVKNLKGLAYMVEKCDEEFLKFHLEYEQIVVLQTEKAKAATPDILTTHCIPSAMSDDLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CEL2</accession>
    <name>A0A166CEL2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0314076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CEL2"/>
    <dbReference type="Gramene" id="KZN03670">
        <property type="protein sequence ID" value="KZN03670"/>
        <property type="gene ID" value="DCAR_012426"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215030"/>
    <dbReference type="OMA" id="QQSCWAQ"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd22153">
        <property type="entry name" value="F-box_AtTLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF119">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="396" mass="44221" checksum="DC1DB2AAA67AA86E" modified="2016-07-06" version="1">MSFRSIIQEMKGELGSISRKGFDVKFGYGSRSRSQRSVQDSSVPVDAFKQSCWANMPPELLRDVLMRIEESECIWPDRRNVVACAGVCRNWREIVKEIVKTPEAARMLTFPISLKQPGPRDYLIQCFIRRDRSTQTYSLYLCGQQASNDIGKFLLAARKVRRPTCTDYIITLTSKDTSKANSIYIGKLRSNFLGTKFTIYDAQPPNAGALVKCHSSRVGLRQVSPRVPAGNHPISHISYELNVLGSRGPRRMHCVMDAIPASAIEPGGVAPTQTEFVRNVDSFPSLPFFRSKSISGSLSTEKDGILMLKNKAPRWHEQLQCWCLNFNGRVTVASVKNFQLVACVDSSAAGLEPENVVLQFGKVGKDVFTMDYQYPISAFQAFAICLSSFDTKIACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CMD4</accession>
    <name>A0A166CMD4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_004927</name>
        <name evidence="9" type="ORF">DCAR_0205453</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CMD4"/>
    <dbReference type="STRING" id="79200.A0A166CMD4"/>
    <dbReference type="Gramene" id="KZN04090">
        <property type="protein sequence ID" value="KZN04090"/>
        <property type="gene ID" value="DCAR_004927"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210266"/>
    <dbReference type="OMA" id="ARAWPWQ"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF58">
        <property type="entry name" value="XYLOGLUCAN GALACTOSYLTRANSFERASE GT19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007871719" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="33"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN04090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG86252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="438" mass="50528" checksum="E83FE01D7BF41964" modified="2016-07-06" version="1">MPPPLLLPLILTLLITSTPTISQDLETECTDQWIYIRQLPSIFNLDLLTNCSQYQTFDNFCPYISNHGLGLKTHNNSHSWFRTDPHMLELIFHRRLLEYPCLTNDPSMASAVYVPYYAGIDALKYLYGQEQNFSFLHGLELYDHIRNVDAPEIWNRHNGHDHFLIMARPAWDFSQPLSNDPPIWGTSFLELQEFYNVTALTFETRAYPFQEMGIPYPTSFHPPNLALFNSWVTRVRRSRRDTLMLFAGGGGISNNPNVRRSIRLECENSTSLRNESYGYSNLCNFVDCSNGVCEHDPIRFMKPMLQASFCLQPPGDTPTRRSTFDAILAGCIPVFFEELSAKLQYGWHLPEAEYEEFSVFIPKEDVVFKGLKIVDVLTSIPRAQVRRMREKVIEMLPRVIYRKHGSSLGMRAKKDAVDIAIEGTLQRIKSRLQEVEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D0A0</accession>
    <name>A0A166D0A0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0206052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D0A0"/>
    <dbReference type="Gramene" id="KZN04561">
        <property type="protein sequence ID" value="KZN04561"/>
        <property type="gene ID" value="DCAR_005398"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="76" mass="8324" checksum="D20130E62487DDEE" modified="2016-07-06" version="1">MVSELIKSVEFLEGDGGVGTVRLVHLGEATELSKTSDSPSCTCFDGMDFKCFDVLKQGEGFSRPMPLHFSYKTVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EAX8</accession>
    <name>A0A166EAX8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_007155</name>
        <name evidence="9" type="ORF">DCAR_0208077</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EAX8"/>
    <dbReference type="Gramene" id="KZN06318">
        <property type="protein sequence ID" value="KZN06318"/>
        <property type="gene ID" value="DCAR_007155"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207026"/>
    <dbReference type="OMA" id="ICTHWYQ"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF698">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="24"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN06318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG88842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="415" mass="45175" checksum="019FBF1BCD3DB8F8" modified="2016-07-06" version="1">MEALSVDSVESQNNISAAEENCQGTSFTRTCFNGLNALTGVGILSIPYALSQGGWLSLIILFVIALLCFYTGLLLRRCMDLNPTIKTYPDIGQVAFGSTGRALISTFMYLELYFVAVEFLILEGDNLHKLFPDVNFHIAGISIPGKQGFVLLAALLVLPTTWLRNLGLLAYISASGVLASIVLVCSVFWVGAFDGVGFDEKGSLWNWNGLATAISLYTFCYCGHAVFPTLCNSMKNRAQFPKVLLVCFVLSTICYGSMAVLGYLMYDGSLMSQVTLNLPTKKLSSKDAIYMTLMNPITKYAIIVSPINTAIEATFPLLKRRSISLFIRTVIVCSTVLVALTIPFFGFVMALIGAFTGTSVSMLFPSIFYLKINKAARKFGVELVIIILIVMMGASVAVVGTYTSLRDIARHAHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EVH4</accession>
    <name>A0A166EVH4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_007865</name>
        <name evidence="10" type="ORF">DCAR_0208891</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EVH4"/>
    <dbReference type="SMR" id="A0A166EVH4"/>
    <dbReference type="STRING" id="79200.A0A166EVH4"/>
    <dbReference type="Gramene" id="KZN07028">
        <property type="protein sequence ID" value="KZN07028"/>
        <property type="gene ID" value="DCAR_007865"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208429"/>
    <dbReference type="OMA" id="ETIVCDW"/>
    <dbReference type="OrthoDB" id="639767at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036469">
        <property type="term" value="F:L-tryptophan decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF96">
        <property type="entry name" value="TYROSINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN07028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="55954" checksum="FFB24685A49DA465" modified="2016-07-06" version="1">MGSINNLTEKNTSEPSNTLDPEEFRRQGHLMIDFLADYYHNIENFPVRSQVPPGYLRDILPDAAPYNPEPIETILKDVQTNIIPGITHWQSPNFFAYFPSSGSTAGFLGEMLSTGFNIVGFNWISSPSATELENIVMDWLGKLLQLPKSFLFSGGGGGVLQGTTCEAMLCTLVAARDRTLRQHGRENIGRLVVYCSDQTHCALQKAAKIAGISPKNIRSIGTSSSTNFQLCPTLLDKTILSDVKNGLIPFYLCVTIGTTSSTAVDPLAPLSEVGKKYALWVHVDAAYAGSACICPEFRHFLDGVENVDSFSLNAHKWFLTTLDCCCLWVKNPSALIQSLSTDPEYLKNAASESNKVVDYKDWQIMLSRRFRALKLWFVLRSYGVDQLREFIRGHVEMAKYFEGLVTMNKRFEVVVPRYFSMVCFRVSVAAVISAGLQSEDINEVNRKLLESVNESGRVYMTHALLGGVYVIRFAIGATLTDYRHVKAAWRVVQEHADVVLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EYU2</accession>
    <name>A0A166EYU2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0209020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EYU2"/>
    <dbReference type="Gramene" id="KZN07145">
        <property type="protein sequence ID" value="KZN07145"/>
        <property type="gene ID" value="DCAR_007982"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG89781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="146" mass="16773" checksum="80384D9C275107EB" modified="2016-07-06" version="1">MGRRAFANMYTIHEADEASIAAACEAEQKLESGEMSGKKSLILKWLAVVAAVLIQCATLVFVAVYSASEQHPGRLLILLLIMLMDPLIFWYAKRMYHETRGYIEAKELIKKAKELQDEMDKLTQRLQKPSRHVVININKLRNSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F148</accession>
    <name>A0A166F148_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_008053</name>
        <name evidence="13" type="ORF">DCAR_0209099</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="7">Binds 2 copper ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F148"/>
    <dbReference type="SMR" id="A0A166F148"/>
    <dbReference type="STRING" id="79200.A0A166F148"/>
    <dbReference type="Gramene" id="KZN07216">
        <property type="protein sequence ID" value="KZN07216"/>
        <property type="gene ID" value="DCAR_008053"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206452"/>
    <dbReference type="OMA" id="ERTPHNT"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004097">
        <property type="term" value="F:catechol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046148">
        <property type="term" value="P:pigment biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016213">
        <property type="entry name" value="Polyphenol_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022740">
        <property type="entry name" value="Polyphenol_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022739">
        <property type="entry name" value="Polyphenol_oxidase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF76">
        <property type="entry name" value="SHKT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12142">
        <property type="entry name" value="PPO1_DWL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12143">
        <property type="entry name" value="PPO1_KFDV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000290">
        <property type="entry name" value="PPO_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0186">Copper</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0883">Thioether bond</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="10">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="11">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="cross-link" description="2'-(S-cysteinyl)-histidine (Cys-His)" evidence="9">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000290-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000290-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000290-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN07216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG89860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="583" mass="65538" checksum="BCE05E0C132EF81A" modified="2016-07-06" version="1">MASFTMPNPFLAKSSQISLTKRPSTTHGFKNFTCKATNGEENPKDELIKSSRFDRRDLLVGLGGLYGATSLSSNPSAFASPVRAPDLSTCDEETVQQIIGNSATRCCPPLSKTMVDFKLPKPGTIRVRPAAHLVDKAYEAKFKKALELMKALPDDDPRSFTQQAKIHCAYCSYAYDQIGLPDELVSAHGSWLFFPFHRWYLYFFERILGKLIDDPTFAMPFWNWDSAGGMSMPSMYTDPKSPLYDKVRDAVHVAPGAMVDLDYGGTDEKITKEQQLKQNLTIMYTAMVSGSSSAKLFLGDPYESGKNVEQSGGGRFESVPHGPVHMWVGDRKQPPGLNMGALYSAGRDPVFYAHHSNCDRLWNVWKTLGGNRKDFSHKDWLDSEFAFYDENAQLVKVKVGDCLDSKKLGYVYQDVELEWLGKRPTPRVKRVLDKFRKKDVARAADFPVVRDVFPIKLDKVTKVLVHRPKKNRTKKEKAKDEEILVIEGIEVEVEAFVKFDVFINDEDEAASAPDKTEFAGSFVNLPHKHKHGKTMKTRLKLALNDLLEDLGAEDDDNVLVTLVPKPGSTCFVNIRNIKIELDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G8Y6</accession>
    <name>A0A166G8Y6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_001215</name>
        <name evidence="3" type="ORF">DCAR_0101271</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG82109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="388" mass="43516" checksum="EE9875F43DB11217" modified="2016-07-06" version="1">MAELLGHGPSNSERVSSLPVLSSNSVVASHGPLLAQQESDKEIYGQQQMWHNQQNGQISMTVSEPATMQLPKKSAMQPSLPNRPLYVPGNCARRFIQYMHQLHRNANASIEFWRKVVAEFFAPTAKKRWCFSLCKNRPKQGATFAQREWHCQVCEQKPGCGYEVTAEMLPTLARVGHETGILQELLYVDMPSEHPRLSGEIAVHYNKATVELVYENLRIAHDGHLCVVFTPDQKITSWDFCIRNVEQYISGRSLLPQLHKLEFLSWNYNRAVRSTSSILQSSELKRHSEKLVEAASGLQKALNMPQVTDVGYTRRYLRCIEISEVVNCMGDVLDLSVATGLSPRMSLAVLGGLSSEDRAVDSNSSRKRPLLQITDRNKKAPNGGQSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GC72</accession>
    <name>A0A166GC72_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_001455</name>
        <name evidence="2" type="ORF">DCAR_0101402</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG82240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GC72"/>
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        <property type="protein sequence ID" value="KZN08799"/>
        <property type="gene ID" value="DCAR_001455"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN08799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="42491" checksum="67BEA0E96AD06F99" modified="2016-07-06" version="1">MEQRTQHTKFAVDLFYMGEVKKLAEDNRFLIDMVGRIQNVRTNIKSIKNDVEKVITKFDLTDGRMLETIEETVSSPPQEIIELQTLTIKEIKAMTPDSTECRVKCQLKVTKVEEQSSWFYAVCTKCPKEISRVDGVFKCEDCNRIIPYPDKSDNTGSIAVIFLDQEVTRIIEKTLFDIEVDAIQENTEGKFPAVLKTFEKKVYTITLNITENNLKKGSMVYEADEIFDKIESSANFDPSTNTDTQMVEAATVDLKDDDLSTPTTGISSTKTRPRVDIEPVAFDPKEDTPAKLNKKDKKKKVFMPNIFSAVYFSSNFCHPIPYFCHPISGVTALYWTSTTTFANEEEFETSVSDTIQSFQDFCYNFSESMTLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IEE3</accession>
    <name>A0A166IEE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="7">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103928</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAP-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IEE3"/>
    <dbReference type="Gramene" id="KZN11044">
        <property type="protein sequence ID" value="KZN11044"/>
        <property type="gene ID" value="DCAR_003700"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205463"/>
    <dbReference type="OMA" id="EEDQMQT"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="256" mass="27658" checksum="DA6881EF049632FA" modified="2016-07-06" version="1">MSIRVLLLVALLLFSAPPFLRVVRCQSESDTEVEVEAAEDGGELGIVDDEVQDYGGGSFVSAPGVETVCVFPKNPSKSVVAGEPTDLLIGLKYEGESNLNVVAVQASIHLPYDHNLRVQNLSSQAFNNASLSASAHATFPYTFAVSKFLQAGTFDLVGIIVYEIDSTAYQSTFYNGTIEVTEAGGPLSVESVFLFSLAVALLVLLGLWVRTQISQFSKKTKRVQKVEVGTETTTASMDEWLEGTAYTQSNKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ISV8</accession>
    <name>A0A166ISV8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0104384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ISV8"/>
    <dbReference type="Gramene" id="KZN11450">
        <property type="protein sequence ID" value="KZN11450"/>
        <property type="gene ID" value="DCAR_004106"/>
    </dbReference>
    <dbReference type="OMA" id="CNIKARY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="284"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="428" mass="47651" checksum="47BD061CE0126387" modified="2016-07-06" version="1">MPKQSKKLFASLVTKNLNAELEFVLPEHVRVQISSLYNSPSPTSYLLLPPTIENVREGYTYPTQVALPFPDQSGTVAATSVSQSGKRKRTTLPSITKDGDDESETAPEVHPPQKKKDANKVNTSSSHFVVHQEMPPAETGDAVLELPSSPQEEKPGRDLLLLLEPAREQDDHQHLMNAASVLKSQLVGRLINHADELSTQEMMMLADKCYRTLEGLGDNYASFNSEVNKLIAQHQELACAAKKKEDWNDCNIKARYLHQVQSLTEVQQRLSSAQDKLSTAKTYGDSLKIRKEELEGELRKLTGELHDVEERVKALTAERDQCKEAHSVAEAELRKLNAEKEEARVAFKAINDEYSAANKEFERMSNHLLQLVVMVIELLGFIMFGTGPPKVQAYSAMKQMSNKLKDPQLPKIGRADGVYANGTVAFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J1A5</accession>
    <name>A0A166J1A5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_004363</name>
        <name evidence="10" type="ORF">DCAR_0104702</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J1A5"/>
    <dbReference type="STRING" id="79200.A0A166J1A5"/>
    <dbReference type="Gramene" id="KZN11707">
        <property type="protein sequence ID" value="KZN11707"/>
        <property type="gene ID" value="DCAR_004363"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195725"/>
    <dbReference type="OMA" id="LGMEMVH"/>
    <dbReference type="OrthoDB" id="683622at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF3">
        <property type="entry name" value="PURINE PERMEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN11707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG85513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="383" mass="42376" checksum="73E0AB1CF35167ED" modified="2016-07-06" version="1">METSSQIHHHPQIKYNNHPLSPPLRGGDVDKNTTIIATNTPNKTYMLLLVFNYMFLFLGSVSSSLLSKFYFNHNGSSRWVSTWVQCAGFPLLILPIFLPFHLFKSTQRKPFSHLSQNLFLYSLLIGLLLGLNNLLFSWGNSYLPVSTSSLLLSSQLTFNLIFSVIIVKQRITFSNLNCVVLLSLASVLLGLGLGSSHDDEPNDQAREKYFIGYFCTIGAGLLFALYLPLMEIIYKQVYCYSMVMEMQLIMEASATVLATAGMAVNGGFSEMRRESELGFDLGHKYYWLTVMGNVVTWQLCFMGTAGMVFLTSGLTGAICMTALTGANVVGGVVVYGDRFDGGKAVSAALCGWGFCSYVYGLYVKSRQGESDRRSEMSTEVVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YCP6</accession>
    <name>A0A175YCP6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Photosystem I assembly protein Ycf3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">ycf3</name>
        <name evidence="8" type="ORF">DCAR_0C036275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="8"/>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Seems to be required for the assembly of the photosystem I complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cellular thylakoid membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Plastid membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Ycf3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093353">
        <property type="protein sequence ID" value="WOH16716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_011069432.1">
        <property type="nucleotide sequence ID" value="NC_087061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YCP6"/>
    <dbReference type="SMR" id="A0A175YCP6"/>
    <dbReference type="GeneID" id="88614255"/>
    <dbReference type="Gramene" id="KZM81223">
        <property type="protein sequence ID" value="KZM81223"/>
        <property type="gene ID" value="DCAR_032485"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:88614255"/>
    <dbReference type="OMA" id="VYYRDGM"/>
    <dbReference type="OrthoDB" id="431027at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Plastid Pltd"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042170">
        <property type="term" value="C:plastid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042651">
        <property type="term" value="C:thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000004">
        <property type="entry name" value="Photosystem I assembly protein Ycf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00439">
        <property type="entry name" value="Ycf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022818">
        <property type="entry name" value="PSI_Ycf3_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002725">
        <property type="entry name" value="PRK02603.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR 1" evidence="7">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2" evidence="7">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3" evidence="7">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH16716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="168" mass="19609" checksum="CFE84714ABEC1533" modified="2016-09-07" version="1">MPRSRINGNFIDKTFSIIANILLRIIPTTSGEKEAFTYYRDGMSAQSEGNYAEALQNYYEAMRLEIDPYDRSYILYNIGLIHTSNGEHTKALEYYFRALERNPFLPQAFNNMAVICHYRGEQAIRQGDSEIAEAWFDQAAEYWKQAIALTPGNYIEAHNWLKITRRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YD38</accession>
    <name>A0A175YD38_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0933498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YD38"/>
    <dbReference type="Gramene" id="KZM81529">
        <property type="protein sequence ID" value="KZM81529"/>
        <property type="gene ID" value="DCAR_029142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="114" mass="13142" checksum="6E7413560ED099B3" modified="2016-09-07" version="1">MKGNKTSDLNKGYARKHGDSEQRPVLLWSKNWIIGRWIALCFLIFFLLVVDVHCFYLLRKYNPEDTVYGLLVMIFGNLVGFGFLGTQARELIEDSNTVNDLPWWCYGLTSSTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YHJ7</accession>
    <name>A0A175YHJ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic</fullName>
            <ecNumber evidence="9">1.3.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0935509</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>protochlorophyllide a + NADP(+) = 3,8-divinyl protochlorophyllide a + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48884"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58632"/>
            <dbReference type="ChEBI" id="CHEBI:83350"/>
            <dbReference type="EC" id="1.3.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.1.75" evidence="9"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YHJ7"/>
    <dbReference type="Gramene" id="KZM82993">
        <property type="protein sequence ID" value="KZM82993"/>
        <property type="gene ID" value="DCAR_030562"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203180"/>
    <dbReference type="OMA" id="QASHWEV"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033728">
        <property type="term" value="F:3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001068">
        <property type="entry name" value="Divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044201">
        <property type="entry name" value="DVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47378">
        <property type="entry name" value="DIVINYL CHLOROPHYLLIDE A 8-VINYL-REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47378:SF1">
        <property type="entry name" value="DIVINYL CHLOROPHYLLIDE A 8-VINYL-REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH15960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="416" mass="45560" checksum="B8EDA0E706A5C5DB" modified="2016-09-07" version="1">MSIYASLNCFTLHSPKAQSFKRLFSSDFISQIQVKSTPIASFSLNSLKRSELVRIKLKPISASISPVVESTSPSFRTKNPRDISVLVVGSTGYIGKFVVKELINRGFNVIAVSRERSGIKGVNSKEETLQNLSGANVCFSDVTSLDSLDKSLEGLGVEVDVVVSCLASRSGGVKDSWKIDYEATKNSLVAGKKYGASHFVLLSAICVQKPLLEFQRAKLKFEAELMKEAEEDNGFTYSIVRPTAFFKSLGGQVELVKDGKPYVMFGDGKLCACKPMSEADLASFISDCVLSEDKINQVLPIGGPGKALTPLEQGEMLFRLVGKKPNFIKVPIEVMDFAIGVLDFLVKIFPGMEDAAEFGKIGRYYAAESMLILDPETGEYNADKTPSYGKDTLEEFFERVLREGMAGQELGEQTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YLW7</accession>
    <name>A0A175YLW7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0830574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YLW7"/>
    <dbReference type="Gramene" id="KZM83832">
        <property type="protein sequence ID" value="KZM83832"/>
        <property type="gene ID" value="DCAR_028746"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197270"/>
    <dbReference type="OMA" id="NIHVAEA"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620731" description="Expansin" evidence="7">
        <location>
            <begin position="19"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="27679" checksum="C3D18BEF84F35561" modified="2016-09-07" version="1">MSFLRLLCIALLCTAATARIPGVYTGSDWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNEGLSCGACFEIKCNNDPQWCHPGNPSILVTATNFCPPNFAQPSDNGGWCNPPRTHFDLAMPMFLKIAEYKAGIVPVNYRRVPCRKSGGIRFTINGFSYFNLVLVTNVAGAGDIQKVYIKGTKTQWMPMSRNWGQNWQSNSVLVGQALSFRVTGSDRRTSTSNNIAPANWQFGKTYVGKNFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YMQ9</accession>
    <name>A0A175YMQ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membrane-associated kinase regulator 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_027693</name>
        <name evidence="3" type="ORF">DCAR_0831796</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YMQ9"/>
    <dbReference type="STRING" id="79200.A0A175YMQ9"/>
    <dbReference type="Gramene" id="KZM84885">
        <property type="protein sequence ID" value="KZM84885"/>
        <property type="gene ID" value="DCAR_027693"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199721"/>
    <dbReference type="OMA" id="IRRSCDN"/>
    <dbReference type="OrthoDB" id="1938320at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019210">
        <property type="term" value="F:kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039620">
        <property type="entry name" value="BKI1/MAKR1/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33312">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33312:SF5">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM84885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH12294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39295" checksum="70755F0A46E995D0" modified="2016-09-07" version="1">MAANIVSCDHADEDYIDMAVSSNDSIFCHAMSSPPHPMEFEFQMFPSFSESDTTTSPADELFYKGKLLPLHLPPRLQMVEKLLQNANCYDKYADSFDEFFSTPLENTTPASTTPFESCNISPYESCQVSRELNPDEYFFEYSTEASDFIADPCKKSWTRKLKLIKQSSIGSKMKASHSYLKSLFNKSGCSNECSAAAAIRERTTPLAKECADNYTKVEREAFGKIHTSKHKISTKRSLKNGQIPQIGGGHHRRSFTGAIKRLSTTKSSSLSSSVGSLSSSSSSTSNGSQESQSFKLNGSSYSDIENSIQSAIAHCKSSQQLNSRKDTSTIEVYSFPSSRIMYEDRERFELCQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YN51</accession>
    <name>A0A175YN51_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_027791</name>
        <name evidence="10" type="ORF">DCAR_0831672</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YN51"/>
    <dbReference type="Gramene" id="KZM84787">
        <property type="protein sequence ID" value="KZM84787"/>
        <property type="gene ID" value="DCAR_027791"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197246"/>
    <dbReference type="OMA" id="PNQKLPH"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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        <property type="gene ID" value="DCAR_027046"/>
    </dbReference>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG82572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="CHROMATIN REMODELER BROMODOMAIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47809">
        <property type="entry name" value="DNA-BINDING BROMODOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00297"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82572.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8C0</accession>
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        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
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        <name evidence="2" type="ORF">DCAR_0102699</name>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Huang J."/>
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                <person name="Yildiz M."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        </reaction>
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            <name>Zn(2+)</name>
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        <text evidence="20">Component of the MRN complex composed of two heterodimers RAD50 and MRE11 associated with a single NBS1.</text>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOG86967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1303" mass="151707" checksum="DB0354E213FB0D0C" modified="2024-05-29" version="1">MSTVDKMLIKGIRAFDPENKHVITFFRPLTLICLKLSCTGELPPNARSGHSFVHDPKVAGETETKGQIKLRFKTAAGKDVVCIRSFQLTQKTSKMEYKAIESVLQTINPHTGEKVCLSYRCADMDREIPALMGVSKAVLENVIFVHQDEANWPLQDPSTLKKKFDDIFSATRYTKALEIIKKLHKDQAQEIKTYKLKLENLQTLKDAAYKLRESIAEDQKKTELLKSQIEEIENNIRSLDAKIQNTESTRKVLEDQQNLISNKKTERSTLLKEKERQYNALEVEIDDSDEDLRRWNSTAEAQTAALKSKICKWEREKEEIAIRAVVHKDNINKRIQEISKLQTEAEACMSLKKERDATIQRLYEMLNLGTLPDVPFSNEVALNLTNCIELRLEDCRKDLEDQRESNKKEIQVVWELYNAATDRWKDVQAQQNAKREIKNGNIKRIEEKEKERYTRELQISDVDLVHIDEREKQMGIDVARKIMQLEQRNFKTLIEQAHLEIVTSTHQLTTLNREKDIMAADSADRRSLSDKKNVLENNRKKHRKIIDEFKDRIRVVLKGRFPPEKDVKKEVIKALRALELEHDDLHVKSREAEMEVNMLQMKKEEAENHLSQLHKDRDSRKRFLESKLKSLDQQTASIDLYLEVLDRARAQRDDKKSKYNMAEGMRQMFDPFERVARAHHMCPCCERSFSAEEEDDFVKKQRVKATNSVERIKALATECTHVDSSFQQLEKLRIVYEEYTKLWKETIPLAEKNLNKLSAEMDIKSQSLDDVLGVLAQIKADKDSVEVLVQPVETADKIFHDIQAQQNEVDDLEAKFDIQGQDTKSMEDIQAEINTLQKKIDNLHNERERLHVEQSDMEGDLHRLQLRWADAREVKVKASSALTELKKIEAELEHLAEEQRQINLEEKHVSEALGPLLKEKQKLLNDYDVTKESLERRYEEQDKCVRSYEMEFRTLLNQNSKIKEYNELKKLELLNNASENKAVLDSQLQNCNTRVEEISAELSKSNELLQNQAGLKRNIQDNLHYRITKQRVDELGYEIELMEEKIAEMGGVSKFEAEVDKLKQERDRFSSESNRYLGTVSVHQSNISKNKIDLKQAQYKDIDKRYFDQMIQLKTTEMANKDLDRYYNALDKALMRFHTMKMEEINKIIRELWQQTYRGQDIDYISIHSDSEGAGTRSYSYKLLMQTGDAELEMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDGPNAESLAAALLRIMEDRKGQENFQLIVITHDERFAQLIGQRQHAEKYYRITKDDHQHSIIEAQEIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WE11</accession>
    <name>A0AAF0WE11_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0206276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WE11"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001725">
        <property type="entry name" value="Kinase like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001893">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0291918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="147"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG87056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="554" mass="63062" checksum="D73999FFA0DF1C9E" modified="2024-05-29" version="1">MPASPLFFTPNRLVSITCSAFVAFTQFLWFLRSGLAVVPRSLRYNGPKTSLQNVTKVYFRKRKRNDAGSQILLGADICGKYPSLVLNISTETQTACAKVIPYFYLLGLIFMKQKLKQNYVENGDHESNSTFLKNQLQLKSLPAFEGFTVEEEEGSGGSGTVYRAKRKCDGVIVALKCPHANASRHHIRKERKMLKKFRGKNFIIEYEGSFKHGSADCLVLRHVEHERPEVLMKEIDLFNLQWYGYCLFRSLASLHKQGVIHRDVKPGNFLFSRRVTKGYLIDFNLAMDMHKKYGTIVHIINMFHYRKLLSGERPREPIPSKGRKELISLAQKTMYGPNDEVASSASIKRKRTAAPAEKVDNNIIYVTPMPLHSSGVAIPGAGFIQSRGQMMNKKEGPCVGTKGFRAPEVLLKSPFQSPKIDVWSAGVTLLCLSTGTSPFDGDPDQNLIDIAKLRGSEDLWEVAKLHDRESSFPVQLLDAKYLLSKKLRDWFKLNTNRPELLEDLPRSFFDLVDKCLTVNPTWRISAEDALKHQFFATCHEELQLQRWLRRQRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHD6</accession>
    <name>A0AAF0WHD6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0207369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHD6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG88135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="138" mass="15769" checksum="8168A76884133361" modified="2024-05-29" version="1">MVSISEGVKVRQQRCASSGWMKFSSHPYNWTIGVMVNEQAFKWLREEADATLHGAQTDNTNLISHVINQNPSQTVHAFSKAYDFMDVDDIILQLRQIKVISWIFTFSLFNKNRRSGLVLGGTGKYSIHPIDYIYEPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHL4</accession>
    <name>A0AAF0WHL4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type E3 ubiquitin transferase BRCA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0208496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHL4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
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        <property type="entry name" value="F5A8.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47776:SF2">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE BRCA1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
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    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="362"/>
            <end position="407"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG89259.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="55874" checksum="8376048CAF34212E" modified="2024-05-29" version="1">MESVIATVSGYHGSQRFNLIKLISHAGGFNVGYLNNSVTHLICWKFQGPKYELAKKLKIIIVNHRWIEDSVRQGRRLPETSYTARWYAILFYLCSGKEVGSLLMEIPAVPDKVSNSVGGQSSAGNASDKHVIDIDCEDAAAAAAAANTFPKMERRQVSKLKRRLVNKTTTRSHSSGATCDDETSSRCYYNEVGIIINSLEKLDSSCTRQLRQKKISSYIEPLRKGRRLVRKNVVSDVLGSESEEECCLVHVPQRQDDTTNLSSTPSATLMNSVGTEPVTKLAKSTDFFNGASTSALYASHQIATPKDARATETVIGSAESTDLSNGASASLIYASPDLLATPKDYSEAEQVTRLNKSTDLSCVICWTSFSSTRGVLPCGHRFCFSCIQNWADYMSSSSKISTCPLCKASFDNITKVDDAVSSDQKIYSQTIPDDPSVNIYVLPTHESPLRNAVTPVCGRCSFREPEDMLIRCHLCHTRCVHSYCLDPYMLPWTCSHCKDLRMLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WI80</accession>
    <name>A0AAF0WI80_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">DUF547 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0209596</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="DUF547"/>
    </dbReference>
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        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
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        <property type="entry name" value="ELECTRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23054">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1, LEUCINE-ZIPPER-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="DUF547"/>
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    </dbReference>
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        <property type="entry name" value="Lzipper-MIP1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="87"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="DUF547" evidence="2">
        <location>
            <begin position="375"/>
            <end position="492"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="303"/>
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        </location>
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            <begin position="77"/>
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        </location>
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        </location>
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        </location>
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            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF14389"/>
        </source>
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            <dbReference type="EMBL" id="WOG90352.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="65169" checksum="A13BE99B1A66840C" modified="2024-05-29" version="1">MDALSVAQGGERACRRESGVSETSTHGHPQHKRSKSASSKNDLSRGGGTNTIRNHNEFHDLRPSKNNVSPRSPLHESPNSSSISAASKHRDSLEKDIEQLQSRLQQERSMRMVLERAMGRASSTLSPGHRHVAAQTKELISEIESLEEEVANREQHVLSLYRTIFEQCVSRSTSEQSSCIASPAHSKNESKKHPSIISSAFCSSKSFPFRPFQALANINGSAKRNSMHSTTRQDSLYAGRSNTHFQHGSPRHGRASDQLSGSEKTSMLRTLKEHLHQCPSKLSEEMVKCMAAVYCWLRSSESTNPEKYQSPVSSRSPANVINPKNGIGQEKERFSTSMVEISWISTDKNNFSRASHALNSYRVLVEQLEKLNLGSMDINAKTAFWINIYNSLIMHAAYNIGGHIISATAIEQSIFCFNTPRVGRWIETILSTAFRKRSGDERQRIKSKYGLQIPQPLVCFALCTGSISDPVLKVYTASNIKEELDMAKKEFLQANIIVKKSKKVFLPKVLERYAKEASISPEDLLQSVANSVDKKLHDSIHKCIVHSDNKKASQCTNWLPYSSKFRYMFSRNLTEKPWWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WI99</accession>
    <name>A0AAF0WI99_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0208887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
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        <property type="entry name" value="CGI-121 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840:SF10">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
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    </dbReference>
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        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG89649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="171" mass="18688" checksum="7E33941474ADD865" modified="2024-05-29" version="1">MNVFEINGATLSLALFTDVTNSKKLLDAMQAGTLEPEVAFLNASIIPDIFPVLAAAHKTLVAKSRESLATRTPHSELVFNYSGSKHIKESFKRCGITEEVTYVLVARFNASVDEIAAIEKLVEGKKVDLTELEKRANHAQIQKQYKITPAELAISSLADSITCRIAARDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJF7</accession>
    <name>A0AAF0WJF7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0208616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May modulate chromatin structure by regulation of nucleosome assembly/disassembly. Could function together with B-type cyclins in the regulation of microtubule dynamics.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJF7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000005">
        <property type="entry name" value="Nucleosome assembly protein11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="304"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="307"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG89378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="379" mass="43009" checksum="6125252932E68417" modified="2024-05-29" version="1">MVNPKKPALDLSDLDGALAADGSALSAEEKSGLVNALKDKLKNLGGPHTDILESLSPVVRKRVEALKDIQSHHDELEAKFFEEKAALEAKYQKLYEPLYSKRYEIVNGIVEPEGVESNDALKQEVGQEKEKGVPEFWVTAMKTNEILAEEISERDEEALKYLRDIKWCRIDNPKGFKLDFFFDTNPFFKNSVLTKVYHMIDDEEPILEKAIGTEIEWYPGKCLTHKILKKKPKKGSKNAKPITKTENCDSFFNFFSPPEVPEDDDDLDEDAAEELQNQMEQDYDIGSTIRDKIIPHAVSWFTGEAALGDDFEDMEDDEDDDGEDEDEEEDEDEEEDDDEDEDEDDVKNRKKSSGSKKSGRSQAAGEGQQGERPPECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WK38</accession>
    <name>A0AAF0WK38_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0209252</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WK38"/>
    <dbReference type="KEGG" id="dcr:108209528"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000076">
        <property type="entry name" value="probable methyltransferase PMT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF984">
        <property type="entry name" value="METHYLTRANSFERASE PMT21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG90011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="594" mass="67760" checksum="2DFF534D88F68045" modified="2024-05-29" version="1">MKHKDGKPIYDKKSRVVPLTVLLVVLCSVSFYLGKVYCPEKDRIVANGIGKAAVSSKTAVNPLQVKSVSFPECGINYQDYTPCTDPNRWRKYDVHRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSKNECWYRNVPYDWINKQKSNQHWLQKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTVRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFALERGIPAILGIISTQRLPFPSNSFDMAHCSRCLIPWTEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTIEDQKSDYEKLQELLTTMCFKLFDKKDDIAVWQKSSDNNCYKQLDSVDIYPPKCDDGTEPDSAWYTPLRPCVVIPNKKRQLSLSSLPKWPERLHSAPERVADVRGGSDGSFKHDDSKWKVRAKHYKKLLPAIGTDKIRNVMDMNTLYGGFAASVIDAPLWVMNVVSSYAENTLAVVYDRGLIGTYHDWCEAFSTYPRTYDLLHADGLFTSESHRCDMKFVLLEMDRILRPNGYAIIRESSFYTDAVATVAKGMKWSCRKEDTEYGVEKEKLLVCQKKLWYSKQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPK5</accession>
    <name>A0AAF0WPK5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0312723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG98530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="903" mass="102038" checksum="088E38297AF0728D" modified="2024-05-29" version="1">MASSDEEEERVPRCVTDYYFEDKQSEPISFAELPLQWRDGESIGGKIESIFLRGKVDNGIRIIHAPATAWKFDLSDSKLKIFVLSKDSWVKLQSPKKSYAENFIRTILITLHVLHYCRNHSRSSFNKALFDHLSQVYRYGRRPSKGDLLNHMDIVREAVKNDKVLARSKLVITLLDEKNEKAKEIKASAEEVEGSSTDDDDDDDDDDDDDDDDESVTGVCDICDDGGFILCCEGECLRSFHPTAEDNCKSLGLASDEVKDICNYICNNCKYKRHQCFICGKLGSSDKDSAVTEVFRCVSGECGRFYHPLCVSQLLHPGSEDEAQMLKERIANGASFTCPIHRCSVCNQGEIERDDQMRFAMCRRCPKSYHRKCLPREISFEPSNDEGIIRRAWDTVLTKKRILIYCLDHEIDMSLGTPIRNHVKFPAMKKKNQASKGLLSGEKAELNKKTPARATNACREKSSVKPPIQTNKKPPATNLCTHKRSICLPERPDSSKKHKTRDIKSCTEGLPTLGAESEKRISALIKDASSLITIEDIYTKHKRLTTNANSSQNAIDKITLGKVQGSVEALHAALQKLDEGCSIEDAKAVCAPGLLRQMTQWKTKLRIYMEPILLGTHYTSHGRHFTKCDKLQKIVDILHSYIQEDDMIVDFGCGANEFSCLVKKKLDDVGKKCSYKNYDIIYPKHDFHFEKRNWMSVHPSELLPGSQLIIGLSPPFGVNASRANQFINKALEFKPKLLVLIVPSETERLDRKTPPYDLLFEDNELLAEKAFYLPGSVDVDGEQMDDWNRPTPSLYFWSHPDWTAKHMAIAQLQGHLCRGPKVSCAELCEDIPKTSTPSRESDHTADVCDDGTRTPRQSDEFCADKIKTSTPRQSEAIRRLDLLGFAPGPYRPQQNSSAGWLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3B4</accession>
    <name>A0AAF0X3B4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phytosulfokine</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-alpha</fullName>
                <shortName evidence="9">PSK-alpha</shortName>
                <shortName evidence="9">Phytosulfokine-a</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-beta</fullName>
                <shortName evidence="9">PSK-beta</shortName>
                <shortName evidence="9">Phytosulfokine-b</shortName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0418296</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes plant cell differentiation, organogenesis and somatic embryogenesis as well as cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">PSK-alpha is produced by endopeptidase digestion. PSK-beta is produced from PSK-alpha by exopeptidase digestion.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Sulfation is important for activity and for the binding to a putative membrane receptor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phytosulfokine family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3B4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009438">
        <property type="entry name" value="Phytosulfokine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285">
        <property type="entry name" value="PHYTOSULFOKINES 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285:SF55">
        <property type="entry name" value="PHYTOSULFOKINES 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06404">
        <property type="entry name" value="PSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041767693" description="Phytosulfokine" evidence="9">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG98950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="79" mass="8839" checksum="BE004ECF02B5E4DE" modified="2024-05-29" version="1">MPTKLSTLFMISLILLFTLTCAARHQPSGVKPQEGMGEEEPKTGEAKCGDGIAEEECMIRKTQEAHLDYIYTQDQKPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3G0</accession>
    <name>A0AAF0X3G0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0519101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3G0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250:SF17">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12360" checksum="B0A2AE9DC8007BED" modified="2024-05-29" version="1">MVYDLRYGSTSGFDWNSENQLVTASRDVWAEYVKSHSGVLKWRSTPFPFFDDLAIIFGKDRATGHNAESAMEAEENINLEEAAQGRNDDSSMQASDEFTSQRSSKRKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3S9</accession>
    <name>A0AAF0X3S9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519249</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3S9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG99893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="54316" checksum="1C438068725265CF" modified="2024-05-29" version="1">MSISLFQNRNRIQEYVTDILRNTDGTFHLKGPWFSNMDLFATCDLTNTHYMLSKNFTNYPKGHDFARIFEILGEGIFNVDHKAWEDQRKTIQFLLGHRNFHTFVKDTTWKKVASGLIPVLDNVLEKRNEVDLQHVFQRFSFDATCILVLGHDPATLTVEFPEVPYEKAFGEAEQALAYRHIVPEFIWKFQKWLRIGMEKKLADAGLAIDNFILHCVKLKNEELQRMPKGGERLDDHHDLLTSYIMDLDENASVFDKSLRDKVLSLLFAGRDTVSAALTWLFWLIATNPFEEYKIHREVSTLLKEKGDGNWRVLDKEELKSMVYLHGAICEALRLYPPVPLQQKTPAESDVLPSGHRLKKHTKSFLSFYSMGRMESIWGKDCLDFKPERWITEKGGIRHEPAYKFSAFNAGPRSCLGKEMTFMQMKIVAASIIFHYKIQLVEGQSICPSHSVILHMMHGLRVRVTRRSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3T2</accession>
    <name>A0AAF0X3T2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0519108</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + succinyl-CoA = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a 24-polypeptide structural core with octahedral symmetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="6"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3T2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="99"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="176"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG99753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="472" mass="51468" checksum="9298BE3C9A732F60" modified="2024-05-29" version="1">MFAVIRRKLTSRGASSALLIGKSVRPALSTPRLSNTALREEVYELFLLCLCHRLTLSSRPTSSYYMVLFGVAVADSNKSFLLKNSSFILYTCLCNIGDLVEAVVPFMGESISDGTLATFLKQPGDKVDVDEPIAQVETDKVTIDVASPEAGVIQKFVAKEGDTVEPGTKIAVISKSGEGVAVSKKPSDEASPKPPATETKTEKKPESTTPPPPAPEKSMEKTPSLPPPRTSASEPQLPPKERERRVPMTRLRKRVASRLKDSQNTFALLTTFNEVDMTNLMKLRSDYKDAFLEKHGVKLGFMSGFVKAAVSGLQNQPVINAVIDGDDIIYRDYIDISIAVGTPKGLVVPVIRDADKMNFAEIEKEINNLAKKATAGSISIDEMAGGTFTISNGGVYGSLLSTPIINPPQSAILGMHSIVNRPMVVGGNIIARPMMYIALTYDHRLIDGREAVYFLRRIKDVVEDPRRLLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8E7</accession>
    <name>A0AAF0X8E7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0521713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <source>
            <tissue evidence="2">Leaf</tissue>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="P:circadian rhythm"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <location>
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        <location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH04389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="16137" checksum="D5B46774B1305E0A" modified="2024-05-29" version="1">MVCIRRATINDLLAMQACNLFCLPENYQLQIYLYHIISWPQLLYVAEDYNGKIVGYVLAKMEEEATPECHGHITSLAVLRSHRKLGLATKLMMAAHSAMEHSNRAAFNLYTETLGYKVHDLEGKYYADGEDAYVMRKIFKG</sequence>
</entry>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GlrX-like_pln_2"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04907.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9657" checksum="3205E2E279DC8D52" modified="2024-05-29" version="1">MALVTRLGSEYPVVILSKTSCSMTHSIKTVLELKWPANVMELKALGRKPSVPAVFIGQELIGGSNEIFSLHLKGKLVPWLLNANAIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XE04</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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    </reference>
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    </dbReference>
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            <end position="65"/>
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            <end position="220"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH06315.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="248" mass="27516" checksum="76B8EC03C2B2A2AD" modified="2024-05-29" version="1">MNPRRSSYTTGGGSQSISIDPNTFFQTQPQTSNVVASLKMFLKKPHAFPFLLFVFLLLTWVSLRLQHSASVSSSAAKTAAFNDQDLKWGDEKKSSHSVEEDYKANMIRFPSSATLSEITKDKRGWLMDPVSVALESGIPGGAQICASLHVGEIRPGSLRGNHRHYTCNETFLIWGAKTKFRLENNLMEKGYTEVMIDADDVAVIVSPSGTAHALVNVDPVRTTFFLGCQDNRIDYNSSTSDFNVWKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DCAR_0624017</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SWEET sugar transporter family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH04606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF142">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="181"/>
        </location>
    </feature>
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            <end position="208"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH04606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="34881" checksum="D242CD6AD8A5E9BB" modified="2024-05-29" version="1">MVAIDFIVGIIGNVISVISYVAPVGQFTEIVKKKSAEHYDGSPYVTSLLSCCLWTLYGILDPDDGVLVVTVNAIGVFSQVIYLALLLLYYPKDKKVKYSGFILVDILFFGVVIAVTMAAFHEESRRTFVGILCASFTVIMYASPLTVVRTVIQTKTAEYMPVFLIFTLWLNGVIWMVFALLLSDFFILVPNAVGILLGTIQLLVYFVYRNSSPSVPEDYSEEMQKGGVLMQATEETEEFPKGVVIAQTTEETNAAAEQKVEAPPTGSNMRNPSLSRMFSFKKAQSGISLSRNASRSVSRSSLQNGFDLESGRSSQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFH7</accession>
    <name>A0AAF0XFH7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0626264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFH7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044718">
        <property type="entry name" value="HOS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47358">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47358:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="51"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="844"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="795"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="899"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="911" mass="102894" checksum="F2E052C5A6067E60" modified="2024-05-29" version="1">MQRHTNDSLVKIISRLKIDRALEHLASIELLDLCNEAKVERCRAPRDLSSCGRAVESVLNTCGHASLCPECSQRCDNCPICRIPVSKNGNRLRLRLYYECIEAGLISKRCDDRFQEKEEAEQLLTADVERLYSLFDVAIENNLVSLICHYVTDVCMDESAVSSDPIVAFLLDEVVVKDWCKRIFRIIVAELHKIYSLSVQEMEEALSSLFRYSVKLSGISNVLDVLESSFKGSLSAQFHDLHQLQECVLKTKQHMEMMIWCIRHHFLEYVRSRHKSYASWRSIVHERKAAAIKRSWPDSIDQSSDTTSNCGSTLFIEEALKNIDIEDGIVNQMGEKSEIATLQKEGFYPKIDGLKGLYPFENLRAGIDSLFLHGSSDMVLAKHAIFLYYLFDRQWSLPDNEWRDIVDDFAATFGITRHSLLESLTFYLLDDYTDEALQEACSILPEISGPTTHPKVAQVLLERQNPDAAFMVLRWSGRDGGAELVSLSEAVTAVRVRVECGLLTEAFMYQRMLCSKLKKLKLRHEPSRNASDVLKDDSQTWMNWVEALVTEICCLCIRRKLVDRILELPWDSDEEKYLHKCLYDFAVNEPLEPAGSLLVVFYLQRYRYVEAHQVDRKLHSLEQNFISDNSVSEEDLSKITLTSQWRAGLVDKSIELLPEVQQQLLRDGKLPELNPPVTEIDSPVTSDPKGQETILNSLLVSSSAKYPFVSQGEIATPLLDPLFSETPSKGFGSVSHSGFKKYGSTLISEVSLLDNARRIQKSMNGYGKDFNSNGITSSATRPSSVIATPLKDFNRSSLRNPGRSQSRDEQISYISLEKDKNKFTNQFKNASQYSRTIAADPVGSSFSGQGFHEGEGTSIEDASANGGPRWRSDDTSEDDEQQTPFPNGFGGNGGGHSISMRRARRSRFAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH27</accession>
    <name>A0AAF0XH27_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop repeat family protein / transmembrane family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0625726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XH27"/>
    <dbReference type="KEGG" id="dcr:108227266"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF44">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="45346" checksum="EB302852B5B118CA" modified="2024-05-29" version="1">MEGFNNSVSMCPVDQHCSQWAEENMSYCLCSTTDLISLTLGVISVLSWGVAEVPQLITNYKEKSVEGLSLMFITTWILGDLLNLIGCKLEPATLPTQYYMALLYTVTTLSLATQTIYYGHIYPRLKANKRHCKIDNVEKRRPHSNDIDKKQADSPRGLEIRSQLLACGVAPSIPIPLLSNDRGSVGGEFYYTSARSLSRSHTPTCGFYMPKRSSSGYECIPAEESLFGEHESTQSTPPSKTKSLMCAVSTLTFFLSISNLRHVDSNAAVMQNQGYVVHMGRKLLQVNESLLIEYGSSGAADIGSYLGWGMAIIYMGGRLPQIFLNFRRGHVEGLNPLMFIFALVGNITYVGSILVSSLDWAKLRPNLPWLVDAGGCVFLDTLILVQFIYFWYHPPQSHESKDESSNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHC0</accession>
    <name>A0AAF0XHC0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0727638</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHC0"/>
    <dbReference type="KEGG" id="dcr:108194260"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000044">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH08201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59828" checksum="8FFA7B388F1F3422" modified="2024-05-29" version="1">MSTTEFFFSSAATTMCAKLNLYDVAFSLFCLFVFSSLVQKLTAKGPMLWPVFGIFPSLIMHSNCLHEWITKALIDNRGTIPFRGLALGGSRGIVTADPVKIEYMLKSKFHNFPKGKYYRERFSELLGEGIFNADDQSWKHQRQAAATVMHSVRFVEYSTESMQDLVHKKLLKVLENLVESKVCIDLQELLLRFTFDNICVAAFGVDPGCMSIGLPDVPFAKAFEEATESTLCRFMVPPFVWKALKFFDLGFERRLKESVKVVHDFAAKTVQERKKELAAQDQDINLDEKFDLLSRLVSLEKGGKNGYFSDKLLEDFCISFILAGRDTSSVGLAWFFWLVSIHPHVEKNILQEIHDILCLRQQKDVKNSNIIFTTDELHKMVYLQAALSESLRLYPPVPFDFKEVLEDDVFPDGTTVKSGDKVLYSIFSMARMESVWGSDCREFKPERWIKEGAFVSENPFKYAVFNGGPRLCVGKKFAYTQMKMVTASVLARYSIQVVEGFEVAPKTTTTLYMKNGLMVRLVPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHR3</accession>
    <name>A0AAF0XHR3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0626691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the transport of sterols.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHR3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000007">
        <property type="entry name" value="Oxysterol-binding protein-related protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000011">
        <property type="entry name" value="Oxysterol-binding protein-related protein 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH07262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43478" checksum="7EFC7D572053DB16" modified="2024-05-29" version="1">MVEDTGDIGGEKEAVLSAPLSLDDEQDTNNNKYRAPNLVKRILSLFTNIRPGSDLTRLQLPPLFNIPKSQLQCYGELVYSNNNHLLSKCGASSISALERFVCVVAWSISAVRPLAFGVTPYNPILGETHHVSTANLNVLLEQVSHHPPVSALHATDQMQNIETIWCHYVIPTFHGTCIEGKVLGSKVLKLLNHSETYVMNSPNLVMRLFPVQGVECLGNVMIRCEETGLEANLMYKGSAVFGRRSSTHRAIQGRIYKSSSTKTLYEIAGHWDKTVTAKDPMSGKLRIIYNAKEAIGGLKTPVVRNPKGLWASESAVVWGEVNKGILSKSWDKAREAKCLIEEKERELVKLRASSAKPWRPKHFNLSYSNETGSWDCSPIQRWVPPAPIAVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHZ4</accession>
    <name>A0AAF0XHZ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">DCAR_0727855</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHZ4"/>
    <dbReference type="KEGG" id="dcr:108195671"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="17201" checksum="819D9943D5380723" modified="2024-05-29" version="1">MLIHEWRAGVKKEAKATYKPQLLLAAAAYYYANGNFTGIPVKYPRASISKNMDWINAMNYDYYVLDQSRGSKKKSDHGLATLGEDLEPERSGSELTWSPSYGYRSSGRRHTVLKFSEIENHNLANKAAVTHDAATVSTYSVAGSTWIGYDDIVI</sequence>
</entry>
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    <organism evidence="11 12">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XS77"/>
    <dbReference type="KEGG" id="dcr:108197770"/>
    <dbReference type="KEGG" id="dcr:108198629"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041894044" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="73"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH11700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH11701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="542" mass="60380" checksum="2E5DA0A33C5616FD" modified="2024-05-29" version="1">MKIQVCSIFTILLLFLTISSAADPNYTSGQDFVQCLIRSSSTSISKVIYTSQNSTYTSVLKFSINNLRFDTPTTPKPIVIVKPVDESQIQTVIYCCKKHDIQMRIRGGGHDYEGLSYVAQVPFVLLDMINLRSISVDPVKATAWVGAGATLGELYYHIGQKSDTLGFPAGIWSTVGVSGLVSGGGYGTLRRKYGLAADNVLDARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLKLVSVPKIVTVFQVERTLEQNATEVLHRWQTVAPRLPKDVEMRVAANPVWKNLPNEPQKTVQDDGSGSLEDSKTVSVNFIGQFLGPKERLLSMMNKRFPELGLVAEACTEVTYIQSILLFSLFSADDSPAGLLNRTAYKIPFKAKSDFVDKPISREGLEGLWKILLQQEPGRTNFLFTSYGGKMAEISESAIPFPHRAGTLYMMYMRVRTDGDTSDSMKWIRGLYKYLTPYVTSSPRAAYVNYNDLDLGVNNLQGTTSYKQASKWGKKYFKNNFDRLVIIKSVVDPDNFFRHEQSIPPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTB4</accession>
    <name>A0AAF0XTB4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0933491</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTB4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF93">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="213"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="339" mass="38615" checksum="C93BE8CF22F06BE4" modified="2024-05-29" version="1">MQHRYSFECLDRSLRDIMKAVDPKRFHMPFGGITIVLGGDFRQILPVIPLAGRGEIVGASITRSKLWKLASVYKLHHNMRLNKGNNREEIEALTHFAQWVLDIGDGKVGRPTNNNKEYGEDDIAVPKKFCNLDSDNSVDQMIHNTFPNFWENYQDPDYLSERAILTPTNQTVGNVNSIIVEKIPGEMSSYYSLDTAEDYPGTQREQLASFPPEYLNSLNIPGLPLHELKLKVGVVVMLMRNLNQTLGLCNGTRMMVTKCLQFCVECENGNSTYITQNVVYKEVFYNLPVIYISCFFHLKKKNASITKVSSSLCSFLYSLTHCIMECFCNPHERHLCHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XU93</accession>
    <name>A0AAF0XU93_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0832997</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XU93"/>
    <dbReference type="KEGG" id="dcr:108199343"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF93">
        <property type="entry name" value="PECTIN ACETYLESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041777387" description="Pectin acetylesterase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="419"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH13487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="419" mass="46646" checksum="A0A40AB96B2E08CD" modified="2024-05-29" version="1">MKMGSILVVLVLVNAVLYGLQAVANPPDLIKIQSAVAKGAVCLDGSPPAYQLDRGKDEGVNKWLLHLQGGYWCTTVEECSMRMNDSSGLGSSNKMTSLYFTGILSDNKELNPNFYNWNRVYVRYCDGSCFTGDKQESLVDGPYYRGGRIFYAIMDELLKLGMENASNVFLTGNSAGGLAATYHCDTFKNILPLSTEVKCLADASFFIHLNNPSGFVNLHGSVTYLPLSCTSMMDAASCLYPRNVVPDIQTPMFLLNSAYDTFQVASAIRLPENSDRSCLYNLTGCPTAVLDAWINFRTKFLQEVTDLGNLSTRGLFINACHTHSQSEFQSKWLGDPDSMLDNMTISEAVGEWYNDNKLIQLIDREHIYPRQCVVDASEPYACNNYTIGASYLCDVNQNKSNIPSVSHSYDRVERKMYYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XW93</accession>
    <name>A0AAF0XW93_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0934916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XW93"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04897">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="35"/>
            <end position="115"/>
        </location>
    </feature>
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        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="334"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH15378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="444" mass="49568" checksum="37E90DF40F303FEE" modified="2024-05-29" version="1">MSFSQDMDDEYEKLIRRMNPPRVVIDNESCKNATVIQVDSANEHGILLEVVQVLTDLNLTVTKAYISSDGGWFMDVFNVTDQDGNKVTDVEVLDYIYKALGPDSDFGYSIRRSVGVKSAVDHTAIELIGCDRPGLLSELSAVLTHLKCNVVSAEVWTHNTRAAAVVQVTDEETGGAITDSERLSVIKQLLCNVLNGSNKSRKAKTVVSHGITHTERRLHQMMFADRDYERNSDEPLEDKSRPEVNVINWYDKDYSVVSIRCKDRPKLIFDAICTLTDMQYVVFHGNVDASGPEAHQEYCIRHIDGSPVKSEAERQRVVQCLEAAIERRVSEGLKLELCTTDRVGLLSDVTRIFRENSLTVTRAEVATRGGKAVNTFYVCGASGYPVDSKIIDSIRQSIGQTILKVKGNTNELNQTTQESPTRFLFGGLFKSRSFCNFGLVRSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1N5</accession>
    <name>A0AAF0Y1N5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylinositol-glycan biosynthesis class X protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0936115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y1N5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042119312" description="Phosphatidylinositol-glycan biosynthesis class X protein" evidence="11">
        <location>
            <begin position="19"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH16559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31470" checksum="B57C13ED3B2DE87F" modified="2024-05-29" version="1">MELLLLKVRICLVFGTIASSFQGYISEPYFEKYGSLHDSYFQDFLAHEVPLGLCEVFQNKINSAPKLSILHRRLIGEGSHRRLSTSIQFEIYQESVSNLPDHYCEAIIIEKLPSGVFADPFELQDLLYRGVYMKASAFGDTNLELPSIQSNRSLVEIHMDIGSNALTGSKHGMETKIELPIHARYPPLGDRGYSRTEFGLPDLFTRCSIKGKSSSQSCIFMSTDSTAKVIDDSIVWEVPSGNKKHASTVSFLTFVSAILSALSIVLVSIRHSNFETDDSKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y394</accession>
    <name>A0AAF0Y394_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0936102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="WOG83730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LRR_N_plant-typ"/>
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        <property type="entry name" value="RLP29"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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            <begin position="21"/>
            <end position="393"/>
        </location>
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            <begin position="26"/>
            <end position="64"/>
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            <dbReference type="ARBA" id="ARBA00004236"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG83730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="393" mass="43394" checksum="0FC0DEF9B51F4416" modified="2024-05-29" version="1">MRVVLLHTLLVSILLHQTLAMLSPVDFLALQSIRKSLQDMPGSHYFASWDFTADPCNFAGVYCSGDRVIALNLGDPRAGSPGLSGHLDPSIGKLTSLAEFTMVPGRVMGILPPAISQLKNLRFIALSRNFFSGEIPAGLGEIRGLRTLDLSYNQFTGNIPWSIGSLPALSNVILSHNRLNGSIPRFGSSSLTRLDLKHNDLSGPIMQDFLPPSVQYLSLSWNRFSGPVDRVLSRLTRLNYLDLSLNRFSGNIPGILFTFPLSNLQLQRNFFTGPVQPVDQVRIATVDLSFNRFSGQISPLFSSVQTLYLNNNRFMGEVPSTFVDRLLDASIQILYLQHNYLTGISLNPTVEIPVQSSICLQYNCMVPPVTMPCPLKAGNQKTRPTGQCIEWKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AM44</accession>
    <name>A0AAF1AM44_DAUCS</name>
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        <recommendedName>
            <fullName evidence="18">Plasma membrane ATPase</fullName>
            <ecNumber evidence="18">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0207494</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cell membrane</location>
            <topology evidence="2 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="18"/>
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        <property type="protein sequence ID" value="WOG88259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AM44"/>
    <dbReference type="KEGG" id="dcr:108205904"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 18" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="714"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="821"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG88259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="950" mass="104461" checksum="650ACCF0F1FAE6FA" modified="2024-05-29" version="1">MQGINRNDASGLEDIRNETVDLERVPLEEVFQQLKCTREGLSSSEAAHRIEIFGQNKLEEKKDSKMLKFFGFMWNPLSWVMEFAALMAIVLANGQGRPPDWQDFLGIIVLLLINATISFYEENVAGNAAAALMAGLALKAKVLRDGKWSEQEAGILVPGDIISIKLGDIIPADARLLEGDPLKIDQSALTGECLPVTKNPSDEVFSGSTCKQGEINAIVIATGVRTFFGKAAHLVDSTNQVGHFQKVLTSIGNFCICSILVGLVIEVIGMLIQQRKYRDIIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSLQGAITKRMTAIEEMAAMDVLCSDKTGTLTLNKLTVDSNLIEVFEKGTGKEHLILLAARASRTENQDSIDAAIVGTLADPKEARAGIKEVHFLPFNPVDKRTALTYIDAKSNWHRASKGSPEQIVALCNCNEDTRRKVHAVIEKFAERGLRSLAVARQEVPEKSKDSLGGPWQLVGLLALFDPPRHDSAKTIRRALNLGVNVKMITGDQLAIGKETARRLGMGTNMYPSSALIGEDVDATLDRDELIEKADGFAGVFPEHKYEIVKRLQEKNHICGMTGDGVNDAPALKKADIGIAVADATDAARGAADIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITVRIVFGFMLIALIWKFDVSPFMVLIIALLNDGTIMTISKDRVKPSPMPDSWKLNEIFASGIVLGGYLALMTVLFFWAVKRTDFFPDKFGVRSLRDSPAEMMSALYLQVSIVSQALIFVTRSRSWSYAERPGLFLVGAFVVAQLIATLLAVYMSWSFAKIQGCGWGWAGVIWLYSLVTYIPLDFLKFAIRYILGGKAWNNLLDKKIAFTSKTNYGKDVREARWAAAQRTLHGLQPPEIFTLASDKSSYTELSALAEQAKRRAEIARMRELKSHVESVVKQKGIDMNTIHHNFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMT1</accession>
    <name>A0AAF1AMT1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0105084</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMT1"/>
    <dbReference type="KEGG" id="dcr:108204114"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF38">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042160034" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="12">
        <location>
            <begin position="27"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="905"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG85891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="952" mass="106652" checksum="2F27757464F218E0" modified="2024-05-29" version="1">MRNSILCFSTICLLLIFLQLQHNLVACSLSPHLQKLALFHFNQTLTISTTSWDCEYVSYEENAVYEIPSRAKTKNWSMSSDHCTWDGVSCDDKTGDVIGLDLSCSQLEGAILPNSALFQLSHLRFLNLSQNDFYLASQFPQEFGFFAKGLTHLNLSNTGLSGRVPSGISHLYKLVSLDLSDNSVSLDLSQNYEAKLEEEVFKSVLQNLTHLEVLNLNGVKISSVLPLNISTSLRVLQLRYTYIHGVVPQEVFHLPNLELLDLSLNDDLRATLPKVKWGSKVTLQHLHLSYINIDGGIPDSIGFLESLVSLDLSSCNLSGPIPRSIGHLVQLTRLDLSQNILNGPVPTFFTDLTDLRYISLWQNNFTGSLPRSLFHHPSLESLSLDNNAFTGQLHEFDSSRSRLQSFSCSNNSLYGSIPHSFSQLVNLTVLDLSSNNFSGVLDLEMFSGLESLKRLHLSDNSLSVRSTIMATLPPNLYSLGLSSCNMKEFPHFSRDANISLNLIDLSNNKIEGEIPHWIGSVGDFYGIPYLNLSHNKLIGGLEQLPWGYIQYLDLQDNKLNGSLPTLMCNSSSLEILNLSHNNLSGVLPICHNNLTQLSVLDLRRNKIQGIIPATLSNFRYLETINLNGNRLQGSIPSSFAELDSLAVLDLGNNQINDTFPLCLEALPKLQVLVLKSNKFHGIINQSSETKHPFPSLRIIDLSYNEFSGALPEKYFRNFKAMMNAEVNKINRSYMEHRYYSDSTALVIKGMDIDLHRILTVFTTIDVSKNNFDGGIAEYFGNLVSLRFLNLSHNYLTGHIPCSIGKLTVLESLDLSSNQLEGEIPQQLTSVYSLAVLNLSCNQLRGRIPEGWQFDTFENSSYAGNFGLCGHPLSKKCESYVRTQEEDDEDEDECHFFCGFTWEAVVIGYGCGVVPAFITGYLMLLARKPKWFAGIIGRELGLKIRRMEIRMRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANL1</accession>
    <name>A0AAF1ANL1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0205827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANL1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009:SF155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32009">
        <property type="entry name" value="TMV RESISTANCE PROTEIN N-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="19"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="115" mass="13241" checksum="50A6A0B09BA164B1" modified="2024-05-29" version="1">MASTSYNQTPSFSTPAPPTTWDVFLSFRGKDTRYTFTDHLYKALLRTGIRTFKDDPELLSGEVISRALPQAIQESKTYIVVLSENYATSSWCLDELVEILNCYNRMKRLVIPVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANN0</accession>
    <name>A0AAF1ANN0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0209073</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANN0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="208"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG89834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="68541" checksum="5C89E773F479B722" modified="2024-05-29" version="1">MAPLELHELKEQLQELLDRGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTIRNRYPLPRIDDLFDQLQGAKYFSKIDLRSGYHQLRVRDEDIPKTAFRTRYGHYEFLVMSFGLTNAPAVFMDLMNRIFHDYLDKFVVVFIDDILIYSRSKEEHEEHLRVVLGILREKKLFAKFSKCEFWLEQVAFLGHIVSEHQRPSGLLQQLDIPIWKWENITMDFVTGLPRTFRKNDVIWVVVDRLTKSAHFLPIRETTPVHELAEIFQRDIVRLHGMPVSIVSDRDTRFTSHFWKGFQRAWGTKLNFSTAFHPQTDGQSERTIQTLEDMLRACALEWTGDWDKYLYLVEFAYNNSWHASIGMPPFEALYGRKCRAPTCWDEVGERDIEGPELVRVTNEKVEQARENLQKAQSRQKIYADQKRKFGGFEPGDHVFLKVSPCKGVKRFGMKGKLSPRYVGPFGVIERVGEVAYRVALPPQLSHVHNVFHVSVLKGYKYHPFHVVQYPLHKIREDLSCEEEAEAILDREERVMRKKNILFVKVLWKNHSEREATWELEDSIRERYPHLFDSGTSFSFVRFRGRNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1APA0</accession>
    <name>A0AAF1APA0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0209375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1APA0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17419">
        <property type="entry name" value="MFS_NPF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG90133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="587" mass="65557" checksum="36E3D2A5237274D0" modified="2024-05-29" version="1">MACLQLSKEASKYKGEEEECTLDGSVDYHGRPAIRERSGRWVAGIIILMNQGLATLAFFGVGVNLVLFLTRVLQQDNYEAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAVFQGIFVIGLVGLSVATNLFLLRPKGCGTPDTPCGSHSSWEVVLFYFSIYMIALGNGGYQPNIATFGADQFDEENMQEAHSKVSFFSFFYLALNLNWFTFLQYHIGFWTSAGSAFAALVLFLAGTSRYRHFKPTGNPLSRFCQVFAAAIKKWRVELSPEEENLYEVDGSKDGSSNGARRILHTNEFKFLDKAAFITSVDFKTGLSNPWQLCPISQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVVQGEAMTSKLSNFRIPPASMSSFDILSVATFIFLYKRVISPLVSRFRKTKTESKGFTQLQRMGVGLIIAVMAMVSAGVVEVYRLKYARKDCLHCSGSSSLSIFWQIPQYALVGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMTSISLGNYVSSLLVTAVMKISTTDHMPGWIPGNLNYGHLDRFYFLLAGLTAIDLVAYIFCAKWYKNIKLESKYDDDDKGKFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQW6</accession>
    <name>A0AAF1AQW6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0208200</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQW6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000161">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032872">
        <property type="entry name" value="WAK_assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF2">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14380">
        <property type="entry name" value="WAK_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042245635" description="non-specific serine/threonine protein kinase" evidence="17">
        <location>
            <begin position="28"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="330"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG88965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="657" mass="74020" checksum="00852CEE50BEAD58" modified="2024-05-29" version="1">MRAIYHCHIHVCLLISVISLFFNRCCCVDQQYEACVPRNCGSLSISFPFFIVGTQEAYCGSPGFNITCVNNTKPLLRIPDYNEALLVDEIFYDNRSLRVSNAALRSNEGSCGAVREIARNLSVRSDHFEYANDSRLVLLWDCSGIEPRLDRYRVGCDGISYLVMKGNDANLKGSTDACKTRAEAAVIDDEQIVTGSNYSTVMRRGFVLKWRATDCTECKLTQGRCGFDIEHDQFLCFCPDRPHYKNCTATPAATMNGKRNSRVILPTAILGASFVLMICLFTIIWMYKGCGNIFTTFLSKKTSSDIDDDISYFGVPVFSYKDLEEATHKFDASKELGDGGYGTVYYGKLKDGREVAVKRLYENNFKRQEQFMNEIEILTRLRHRNLVLLYGCTSHRSHELLLVYEYIPNGTVADHLHGDRAENEPLTWHVRMHIALETASALAYLHKSDIIHRDVKTNNILLDKNFTVKVADFGLSRLFPTAATHVSTAPQGTPGYVDPEYHQCYQLTDKSDVYSFGVVLIELISSMPAVDISRSRNEINLANLAVNRIQKCAFDELIDPSLGYKSDPVIERMTTSVAELAFRCLQLEKELRPAMDEVYECLRQIQVVDESNKAKPQPSPESENVVLLKNKTCPASPVTVIDNWMSSRSTTPSVSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZ03</accession>
    <name>A0AAF1AZ03_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0519940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AZ03"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH00574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12820" checksum="1E7F79EDA4A621F1" modified="2024-05-29" version="1">MEAEYVAASKAAKEAIWFRNFLLDLGVVPNLPQQITIYCDNTGAVANTKEPRAHKAAKHIERKYHLIRQFVKRGDIIVADIASKDNRADPFTKSLPAKAFQEHVEAIGVRYLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AZB5</accession>
    <name>A0AAF1AZB5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0417487</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG98146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:defense response to oomycetes"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
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    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
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        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    </dbReference>
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        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
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    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="673"/>
        </location>
    </feature>
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        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="351"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="378"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">GYF domain-containing protein</fullName>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    </reference>
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            <end position="61"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B312_DAUCS</name>
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        <recommendedName>
            <fullName evidence="19">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000001">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000420">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF15">
        <property type="entry name" value="DICER-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14709">
        <property type="entry name" value="DND1_DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="14" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="RNase III" evidence="19">
        <location>
            <begin position="1214"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS00517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH02768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1623" mass="181598" checksum="AA05DF84DB109C56" modified="2024-05-29" version="1">MASGDSDSNTLSVVSIDEEGHHGSDEKNPTLIARQYQTKLCQKALSENTIAYLGTGCGKTHIAVLLIHEMRHLIKKPQNNICQAKVIKDSIDVKVGIYCGSSKHLKRHTNWEKELAQHEVLVMTPQVLLQNLSHCFIRIELIALLIFDECHHAQAVSNHPYAEIMKVFYNSNATKLPRVFGMTASPILGKGASVSGLESLLRAKVYSVEDKEELERFVTSPKVSVYYYTTAADGPSSPLVYYSKLEGIKSQCVSTLVENISDMDSLKTSKKMLQKLHTNLCFCVEKLGVWGAFQAAHILLKGDFVVQNELLEMEGQNERMEMGEQSSHVSICDKYLSQAAIMFLSDCQKDHTKKKLNSPEVLQEPFFSKKLLQLIDILSNFRSQQNMKCIIFVNRIVTARSLSSILQSLKVLSAWKCDYLVGVHSGLKSVSRKSTNALLAKFQSGEINLLVATKVGEEGLDIQTCCLVVRFDLPETVASFIQSRGRARMPQSEYAFLVDSGNEKEQNLIDSFSKAEDKMNEEIEFRTSTATVCDTDEKTYRVELTGATISSGSSISLLYRYCSKLPHDEFFKPKPDFSYFDEADGTVCQIILPSNAPIHQVSSAPQSSKDAAKRDACLEACKQLHQLGALTNYLLPEQDVENEDLESLSDSDCSDDEDTRRELHEMLVPAVLKEPWSKAEDYVYLTSYFVKFRPLPPDRDYKPFGLYVKVSLPGEAERMKLDLHLARGRSVVTELIPSRTMLFSRDEIALAEKFQEMFLKVIIDRSEFNSEYVPLGRIDFDILAPRTYYLMLPVIWNEYEEAMIVDWNLVMRCLSSPIFNMPEVAKANGLSQPSELLHLANGPKSVHDVVNSLIYVPSKKLFYFVSDVLSEKNAYSDYKASKSHVHHFSEKFGVHLLHPRQALLKAKQLFCLENLLRKKGNLESREKEEHFVELPPEICELKIIGFSKDIGSSLSLLPSVMHRLESLLVAFELKDLLSVSFPEGAEVSASRVLEALTTEKCNEHFSLERLEVLGDAFLKFAVGRRLFLLHDALDEGQLTRKRSSVVNNSNLLKLAVANRLQVYIRDQSFDPCQFFALGRPCSVVCSAETEKSIHSSHCSGAENSDIELRCTKSHHWLHKKTIADVVESLVGAFIVDSGFKGAAAFLKWMGIKVEFEDSKVSHICSASSIYLPLAAQIDIAALEDSIGYQFNNKGLLVQAFVHPSYSYHYGGCYQRLEFLGDAVLDYLITSYLYSVYPNLKPGQLTDLRSTCVNNICFANIAISRSFYKYIISESSGLCKSMEKYVLFSRTHQLDGNLVEVPPCPKALGDLVESCIGAILLDTGFNLNHVWKTMLSFLDPVINFSGLQLNPIRELQELSQSYNMELEFASSKKDNTYIVDIKVKGKNVCEHSCASNISKKAAKRRAAKQVIRILKELGYKPKSKSLEEVLKSTHKVEAKLIGYDETPIDVNAQYAIARNEKIPTDVSSPYAAICSNETATNVRSPNAAVLDNLKVQDSRTSGASSSNVHSLGVEPHRSGGINIQPIMPINSLGCSMKKSAKSHLFEICAANHWKPPKFICCKETGASHLKEFTYKVIVKPDLGNCFIEAIGKPAGKKKEAAENAADGAVWLLKEHFFVGQNLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B356</accession>
    <name>A0AAF1B356_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0521646</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B356"/>
    <dbReference type="KEGG" id="dcr:108224071"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000005">
        <property type="entry name" value="transcription initiation factor IIA large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH02257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="389" mass="42155" checksum="8AE056E506EBD710" modified="2024-05-29" version="1">MASSTATSGVYIQVIEDVINKIRDEFVSNGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAAPRLAPGGPITPVHDLNVPYEGTEEYETPTADMLFPPTPMQTPMQTPLPGSGQTPLPGTVQTPGTAELYNIPTGGTPFTPGEYPSENAGGVSEMKPGRPSQFMQPPAPWLNQRPPLDVNVAYDEGREEIERGASHEPTTQDFFTMSAGKRKRGDFPSQYNNGYIPQQDGAGDALLDASKEATAANLEFGRGVSMSSRIPQVDGPTPDPYDDALSTPNIFNYQGVVNEDYNIVNTPAPELQAATPAAVAQNDVLDEEDDDEPLNENDDDDDLDDVDQGEDLNTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4J0</accession>
    <name>A0AAF1B4J0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0623767</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4J0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000009">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF153">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH04358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="504" mass="55139" checksum="04235BBB3E8EB6AA" modified="2024-05-29" version="1">MGDCKLQVSISSQSNSPSLSSSPDNGSPCSPSDIVTSPMPSKFTDVPGGAISEKKEYPIDPSLPDIKNSIYSTDEFRMFSFKVRPCSRAYSHDWTECPFVHPGENARRRDPRKFHYSCVPCPDFRKGACRRGDMCEYAHGVFECWLHPAQYRTRLCKDGTSCDRRVCFFAHTPDELRPLYVSTGSAVPSPRSSASAASVMDMAVAMNLLPGSPSASLMSPSGFNQPMSPSGNGAHSSLAWPQPNVPTLHLPGSNIQSSRLRSSLSARDVPPEDLHLLEDMDAHQLLLNDLACLSQSRPSPVSLNRSGRPKTLTPSNLEELFSAELASSPRYSDQAASASGFSPSHRSAVFNQFQQQQNMLSPINTNVFSPKSADHPLLQASYGVSSPGRMSPRSVDPISPMGSRHTAFSQREFQQPQQMRSLSSRDLGSNNVSIVGSPVNSWSKWSSPSGKVDWSVNGDENSWLPRSSSNDLNNNTEEPDISWIQSLVKESPPEMKDKTASFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BDH4</accession>
    <name>A0AAF1BDH4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0935252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BDH4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="64"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH15709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="342" mass="38531" checksum="5B39E77D9D594340" modified="2024-05-29" version="1">MMEPRPAPPLQDVDNQELKKLLSSLPKERGIISSDTYQYQGFWYPPHALQGIINCQEEFQPRENDIFLVTPPKSGTTWLKAILYALVNREAHPPQDPHHPLLSKTPHQLVPFLELLHPSEYVSVCNSSDSSTRILATHVPLGTLPKAITGDSSSLNCKIVYLCRDTKDNFVSLFHFGNDRMKTSPISLEDAFDLYCKGISPGGPIWDQILGYWKESQKNPHKVLFMRYEEMKDEPYLQLRRLANFLGIPFFPEEENSGLVDQIISICSFESMTKVDVNKTGTFVDGSNNSSFFRKGVVGDWKNCLTADMASRLDQITEEKFRGSGLSLSNISSPCSPSPCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BES3</accession>
    <name>A0AAF1BES3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0933881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BES3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042152164" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH14362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="119" mass="13158" checksum="EB9516D0C0885AD2" modified="2024-05-29" version="1">MVRITGFLAIVLALIVSITLVAEAEHTGFECNGSKNQKALDACSPFTWRGAKEPSGECCSRFRAFIETAKTAEERRELCSCVQRNTIVYKAIVKNVDALWGICGVPFLFSSDSKFDCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BGE0</accession>
    <name>A0AAF1BGE0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0933577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BGE0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="384"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="489"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="524"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="559"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="70133" checksum="3356265A96501A12" modified="2024-05-29" version="1">MSRMRSAFTACFTSNAIHDKGFLGFLRHGTPALTTTSSCCRVIALLRLHSLSQSPTCPNPNHIPNHLTKLQQLLLEKSRVGFDKPDDALRVFDKMHRMKPLPSVVDFTQLLAALVKTKHYSVAVSVFSKMCSFGIPVNIVTYSTVINCCCHLKHIDYAFALLAAIFKRGFIPTIVTYTTLVRGLVSQDKFLEAELMFSYLLQHNQIEPDAVSFNTIIDGLCKTGNTSQAMRLLRYMEKKGCMPCVVTYNTIIDSLSKDRLVDDALGLLAEMSDKGIQPSVVTYNSLIHGICNLDRWDDISKLFRDMDRHKISPNMHTYSILVDAHARDGKVKDAEYLIELMIRRGQHPDVVTYSALMDGYCLRGELDKALVVLSTMISKGILPDTQTYTILINGYCKKMKVDTAITLLEEMPMKGLTPTVETYSTILDGLFRTRRLDEAHMFYRKMLNQGVKLNIVTCRILLHGLCCNGLAGEAMSVFHDMESCGLLPDIHIYNILIHGLFKDGKVDEARSLFHNLRLKGLRPGVISYTIMIQGFCQEGLLEEAYKILLEMEENNCLPNEVTYNTLIRGCFKNKMYDGACVLIDEMCAHGFKADASTTSMLFDLLQSKEQDPALLAMQKKFMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BJE3</accession>
    <name>A0AAF1BJE3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Helicase-like transcription factor CHR28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0935239</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BJE3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000068">
        <property type="entry name" value="SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000071">
        <property type="entry name" value="SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF24">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR CHR28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="296"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="727"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="831"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="50"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="457"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOH15696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112958" checksum="FF964947B8166A19" modified="2024-05-29" version="1">MLDLMFVVLVFECGKGTYFLWEMDFLEISSSDADSDSDWDLDEIRASYDQIDSTDSVNPGDNLSTVDNARPNLIDNKKRSRKLHSQTAVQDRSSLDHSHQKKAGNHRSSDTTSNSVLLNLRTTQDTNSTSSARKDYDQFTSQQGSKRSLPNSIQPQGPNPRSFNLMENVGSSQMRETYGNTFSAPQKLIHGMELVNRTGQGSVDKFMSTTSGSRILPLSMMHGKYTSTSQHANSVDPVFRSTTGEDRAEYDERLIFQAALQDLNQPKLEDTLPEGHMTVSLLRHQKIALAWMLKKETKSPHCAGGILADDQGLGKTISMTALILKQKYLQSITRPEVASQMTAEALNLDDDEDNEITVLDQGKLDGESNDLKLLPEARTSRQEFKSLRPQAGTLVVCPASVLRQWARELDEKVAAEVSLKVLIYHGSNRIKDPDELAAYDVVLTTYALVANEVPKQPVVNEDDDDPKNGKRNGLFDEISTNKKKNKMTNSRKRKWKGKRRNGMSDVDLDSGALARVRWLRVILDEAQTIKNHRTQVARACCSLKAKVRWCLSGTPIQNSIDELFSYFRFLRYEPYNVYKSFCGMLKHPISLNSIQGYKKLYAVLRTVMLRRTKGTVIDGEPIITLPSKTINLKKVEFSVEERAFYQRLEAESRSQFKAYAAAGTLGQNYASILLMLLRLRQACDHPLLVKGSTSDSVERFSSEMAASIPKDMLINLLKRLQSTSAICGVCKDPPEDAIVTMCGHVFCYQCVSENLTGDDNICPSPRCKEELGPDLVFSKATLRSCISADQDDNPSSSSLHEKSRVVQRDYISTKIKTAMEILMSHCKSYGGISSRREELSETGGVCSDAQIEAPRKAIVFSQWIGMLDLVEMALLRTDLQYRKLDGTMTLTARDKAVREFNTDPEVTVMLMSLKAGNLGLNMVAASHVILLDLWWNPTTEDQAIDRAHRIGQTRPVTVSRITISNTVEDRILGLQEEKRKMVASAFGEDPSGGTGSRLTVEDLRYLFMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X5B7</accession>
    <name>A0A161X5B7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0728413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X5B7"/>
    <dbReference type="Gramene" id="KZM87613">
        <property type="protein sequence ID" value="KZM87613"/>
        <property type="gene ID" value="DCAR_024729"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193352"/>
    <dbReference type="OMA" id="LMWANIQ"/>
    <dbReference type="OrthoDB" id="5594417at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH08962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="25225" checksum="F8A948800906B868" modified="2016-07-06" version="1">MSFLFGKRKTPAELLRENKRMMDKSIREIERERQGLQNQEKKLILEIKKSAKQGQMGAVKVMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPSLQKIMQEFERQNEKMEMTSEIMGDAIDDALEGDEEEEESEELVNQVLDEIGIDINNELMNAPSGSVAAPAAQNKVAAQAEGAGPDDGVIDSDLQARLDNLRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XRD1</accession>
    <name>A0A161XRD1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="4">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_017411</name>
        <name evidence="8" type="ORF">DCAR_0519910</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XRD1"/>
    <dbReference type="STRING" id="79200.A0A161XRD1"/>
    <dbReference type="Gramene" id="KZM94166">
        <property type="protein sequence ID" value="KZM94166"/>
        <property type="gene ID" value="DCAR_017411"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221913"/>
    <dbReference type="OMA" id="DHREKDG"/>
    <dbReference type="OrthoDB" id="1933483at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000002">
        <property type="entry name" value="Gamma-glutamylcyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM94166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH00544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="183" mass="20644" checksum="10C5662BC80A518B" modified="2016-07-06" version="1">MVMWVFGYGSLIWKAGFKYDDRLVGFIKDYRRVFFQGSTDHRGTPDYPGRTVTLEPAQGQVCWGIAYKVSSKEDEEAALLHLEVREKQYDKKAYLDFYTESGASTPALSGVMVYIASPDKKLNKNYLGPASLEELARQIIKAEGPSGPNRDYLFHLEKALLQLGCEDKHVTDLANEVRKLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YDM7</accession>
    <name>A0A161YDM7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0624421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YDM7"/>
    <dbReference type="Gramene" id="KZM90849">
        <property type="protein sequence ID" value="KZM90849"/>
        <property type="gene ID" value="DCAR_021786"/>
    </dbReference>
    <dbReference type="OMA" id="SGCKSNW"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF3">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="613" mass="70394" checksum="93CF0A623CA005AC" modified="2016-07-06" version="1">MGVFVREPISRNYLNLKLLLFNALHSHADRQTLVLRRRSTRPEQQQLLHGSSLPLSSLFLSAYCKIYSCLLSHPAPLNLVQDTKLLNSRALFDFNLGFKGSFGVSSFVGLRKDLIFRPRGFCSVGGYDDCSDLEDEGDDVGEGESGSVKSEADLREVERVCKVIEELSAWNRKMVSGLNECGINLSHDFVVDVLDRFKHARKPAFRFFCWAGNQEGYVHDSRTYNVMMRILGKARQFETMVSLLEEMGKKGVLNIETFQIAIQTFGASQERRKAVAMFELMKKYKFKVDVDTINSFLDALGRAKLGKEAQVLFGELEERFTPNLQTYTVLLNGWCKVKNIMEAGRVWNEMTDKGFKHDVVAYNTMLRGFLDDKKLPDAIKLFEVMKTKGPTPNVQSYSIMIRYLCKQKLIKRAVGYFEEMASYGCEPDAAVYTCLITRFGNMKKMDKVFGLLKQMKERGCPPDAQMYNALIKLMTRCKMQKNALRIYKEMLQNGIQPTIDTYNILIKSFFQTKRFGMGFAAWEDMKRRGCCPNYNSYNLLIRELMKQGRSIDACKYLEEMIEKGMEAPQIDYDKFKSDCSRAGKPHILEELAQKMKLSGWHGNKEKMGRVEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YGM0</accession>
    <name>A0A161YGM0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0625976</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YGM0"/>
    <dbReference type="Gramene" id="KZM92209">
        <property type="protein sequence ID" value="KZM92209"/>
        <property type="gene ID" value="DCAR_020426"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225144"/>
    <dbReference type="OMA" id="PTHVAEC"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08277">
        <property type="entry name" value="liver_alcohol_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000067">
        <property type="entry name" value="alcohol dehydrogenase 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF38">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="10">
        <location>
            <begin position="35"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="205"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH06548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="377" mass="40200" checksum="669B549CB8444244" modified="2016-07-06" version="1">MSHYIPQVITCKAAVIWKAGEALKVEEIKVDPPKTGEVRIKMLFASVCHTDILYRDGFDQTLFPRALGHEGVGMVEAVGENVKNVLIGDIVMPVYLGECGSCLNCKSGKSNLCHVYPLSLTGLLPDGTSRMLISGTGERVYHLISCSTWSEYTVFDSNYLVKVDPKLPLPHASFLSCGFTTGFGAAWRTASVEEGSTVVVLGLGAVGLGAIGGARTQGASKIIGVDLIDLKGKKGTKFGMTDFINPKDSGKSISELVKEATGGLGVDYCFECTGVSSLLSEAIESTKVGLGTAVLIGTGTDPIGHFNIVPFLCGRTLKGTVLGNVRVQSDLPTIINKSVNKEIDLEELLTHEVSLDEINEALEITKQPDCVKVLIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5P2</accession>
    <name>A0A162A5P2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_019002</name>
        <name evidence="9" type="ORF">DCAR_0521748</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A5P2"/>
    <dbReference type="STRING" id="79200.A0A162A5P2"/>
    <dbReference type="Gramene" id="KZM95760">
        <property type="protein sequence ID" value="KZM95760"/>
        <property type="gene ID" value="DCAR_019002"/>
    </dbReference>
    <dbReference type="OMA" id="QITHIVM"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF14">
        <property type="entry name" value="CHALCONE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="7">
        <location>
            <begin position="237"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM95760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH02359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43442" checksum="F86B13C8714364CD" modified="2016-07-06" version="1">MGSISVEQMMEKQRAKGPATILAIGTATPPNSYLQADYPDFYFRVTNSEHKTQLKEKFKRICDKSMIKTRYLHITEEILDENPNMRNYSAPSFNARQEMLRMEVPKLGREAALKAIKEWGHSKSEITHLIFCTTSGYDMPSADYQLTKLLGLSPSVKRHMIYLQGCFAGGTVLRLAKDLAENNKGARVLVVCAEITTITFRGPHLESLLPQALFGDGASSVIVGSDPDPLTERPLFQIVSSAQHILPDSEDTIRGKLGESGLMFFLKKNITTLIASDIEKLLKEAFEPIGISDWNSLFWITHPGGPAILNQIELVLGLKEEKMWASRKVLSQYGNMASACVLFVLDEMRKKSMKDGMATTGDGLDWGVAFGFGPGLTVETVVLHSMPVTSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5P7</accession>
    <name>A0A162A5P7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_019012</name>
        <name evidence="14" type="ORF">DCAR_0521758</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM95770">
        <property type="protein sequence ID" value="KZM95770"/>
        <property type="gene ID" value="DCAR_019012"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223614"/>
    <dbReference type="OMA" id="FRRDIPY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009914">
        <property type="term" value="P:hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF44">
        <property type="entry name" value="SULFATE-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="688"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="773"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1201"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1239"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1279"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1314"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1344"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1376"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1425"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="174"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="855"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="810"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM95770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH02369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1456" mass="165553" checksum="FD44E3DE9CB9B16D" modified="2016-07-06" version="1">MEASGAMAGEDLMRSMSRRASFAASASKRGWGASSIREAFTAAGGDVFQKSGRQEDEDDLKWAAIERLPTYDRLRKGFLQQVLENGRVVHAEIDLTHLGAEDKKHLMENVQRVIDDDNERLLHRLRDRIDRVGIEVPKIEIRFEQLSVEGDAFVGSRALPTLLNSTINSIEATLGFLRLFPSKKRVVNILHEVSGIVKPSRMTLLLGPPGSGKTTLLKAIAGKLDLDLRETGKITYCGHDMREFVPQRTCAYISQHDLHHGEMTVRETMDFSGRCLGVGTRYEMLAELSRREKVEGIKPDPEIDAFMKAISISGQETSLVTDYVLKILGLDICSNTMVGDEMRRGISGGQKKRVTTGEMLVGPAKVFLMDEISTGLDSSTTFQIVQYMKQMVNIMDVTMIISLLQPAPETFDLFDDIILLSEGQVVYQGPRECVLEFFEYMGFKCPDRKGVADFLQEVTSLKEQEQYWFRKDEPYRYVSVCEFVHRFNSFHVGRKISAVLSSPYDRSRAHPAALVNDKYGISNKELFKACLSREWLLMKRNSFVYIFKTTQITIMALFTFTVFFRTEMKYGEIDDGGKFYGALFFSLINVMFNGMAELALTMFRLPVFFKQRDALFYPAWAFALPIWLLRLPLSVMESGIWILLTYYTVGFAPSASRFFRQFLAFLGIHQMALGLFRFIAALGRTQVVANTLGTFTLLLVFVLGGFIIAKDDLQPWMKWAYYLSPMSYGQNAIVINEFLDPRWNTYISDSMYPDHTAGKVLLEARGMFVEDHWYWLCVIALFAFSLFFNICFVAALTFLNPLTETKSVASEDCDEADKKKRPSSEETDMAEMNSNTENNVVDRKGMVLPFEPLSLAFDHMNYYVDMPAEMKSQGIEEDRLQLLRNVSGAFRPGVLTALVGVSGAGKTTLMDVLAGRKTGGYIEGSISISGYPKNQETFARVSGYCEQNDIHSPHVTVYESLVYSAWLRLAADVDEEKRKMFVEEVMDLVELNSLRNALVGLPGVDGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRAVRNTVDTGRTVVCTIHQPSIDIFEAFDELFLMKRGGQVIYAGPLGHYSQVLVDYFESIPGVTKIKDGYNPATWMLEVSSSAIEAQLDVDFAEIFTNSDQYRSNQELIKDLSTPKSGSKDLYFPTQYSQPFITQFKACFWKQHWSYWRNPQYNAIRFFMTAVIGLIFGIIFWKKGDKMHKQQDLSNLMGAMYAAVLFLGGTNTSAVQSVVAIERTVFYRERAAGMYSPLPYAFAQVSVETIYVGIQTFLYSLILYSMIGFHWEITKFLWFYYFVFMCFVYFTLYGMMLVALTPSYQIAAIVMSFFLSFWNLFSGFLIPRMQIPIWWRWYYWGSPVAWTIYGLVTSQVGDKMDIVHVPNHGEIPVKDYLKVFLGYEHDFLGYVALAHIGWVLLFFAVFVYGIKVLNFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ABR9</accession>
    <name>A0A162ABR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_014128</name>
        <name evidence="24" type="ORF">DCAR_0416816</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ABR9"/>
    <dbReference type="STRING" id="79200.A0A162ABR9"/>
    <dbReference type="Gramene" id="KZM98510">
        <property type="protein sequence ID" value="KZM98510"/>
        <property type="gene ID" value="DCAR_014128"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218855"/>
    <dbReference type="OMA" id="PRCDIEG"/>
    <dbReference type="OrthoDB" id="1913956at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000444">
        <property type="entry name" value="probable L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000320">
        <property type="entry name" value="probable L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007831092" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="360"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="674"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="676"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KZM98510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG97476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="696" mass="77188" checksum="14448E3DDABA55C9" modified="2016-07-06" version="1">MVAPPGLNAAATAFLFLLTITVVRSDKLKTFKGSYGVTRSFDDNIDYYYRILKLDKNATIHNNAIQLTPDSSGSGDIVPKQNQSGRAFLPQKFKLWNGDSNTTRLASFNSSFLINIFRPTNDTPGEGLAFFIAPNLNLPLNSYGQYLGLTNASTDSEASNKIVAVELDTAKQDFDPDDNHVGLNINSVRSVKTTSLSRFGIELVPKGTRLYNVWVNYDGVKKVLEVYIANSSIDGPTPAMPGEPVLKQDLNLALYVEQESYFGFSASTGSDIQLNCVRKWNLTVTSYVKDEVPVLAIALGVGIPVVVLLVVGAGFMVVYLRKKRRKRGNISSNILGALRSLPGTPREFKFRDLKKATNNFDDKFKLGQGGYGVVYRGVLPDEENLEVAVKWFSRESLKGEDDFLAELTIINRLRHKHLVRLLGWCYKNEKLLLLYEYMPNGSLDMHLFAEPGKQPLSWALRYKIVSGVASALHYLHNEYDVRVVHRDVKASNIMLDSNFNARLGDFGLARALDNEKTSYMEAEGVLGTVGYIAPECFHTGRATQRSDVYAFGAVLIEVVCGQRPGTKIGGFQFLVDWVWFLHRDGRILDAVDERLAGDYVAEEAERLLLLGLACSHPIAHERPKTQTIVQILLGNMPVPHVPPFKPAFIWPAMGPIEDDDLSLATTLDTTSMTTSNLGSAWSTQNMGKDSHEQSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AFT0</accession>
    <name>A0A162AFT0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_009015</name>
        <name evidence="9" type="ORF">DCAR_0310202</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AFT0"/>
    <dbReference type="Gramene" id="KZN00261">
        <property type="protein sequence ID" value="KZN00261"/>
        <property type="gene ID" value="DCAR_009015"/>
    </dbReference>
    <dbReference type="OMA" id="FTICAIH"/>
    <dbReference type="UniPathway" id="UPA00213"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF45">
        <property type="entry name" value="SCOPOLETIN GLUCOSYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN00261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="486" mass="55000" checksum="51794E8A97DBB6CF" modified="2016-07-06" version="1">MSTSRPLEIYMLPFLSFSHMIPLSEIGHLFSSAAQNVTILTTPHNASSLLKKTTDSPNFRVQTFPFPAKQVGLPDGLENFLSAKDIPTASKLYAAMTLLQADLESFMISNPPDVIVSDMFFPWTADFAAKISVPRIVFQGVCMFAQTLKHEVRKSDSPHHSVESDYELFVIPNLPHKITMTRSQLPDYIRTPNGYTQLMEQWREAELKSYGIIVNNFSELDSVYTDYYKDATGGKIKIFHVGPTSLLNSNSNNKMERGHETVVTDNDRLNWLNEKNFNSVIYVCFGSACVFPDLQLMEIACGLESCGKDFIWVVFGKDEEKDDDMIKWTPSGFYQNVIKTKRGMIVRGWAPQVLILNHPSVGGFVSHCGWNSVIEAVSCGVPMVTWPLYAEHFYNEKLLTQVYGIGVEVGAEEWNLWVDSGKKIVRREKIEEAVRKLMDGEDETVKEMRRKIEELGDVAKKAVQEGGSSHKNLMVLIEELKKLRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B1P5</accession>
    <name>A0A162B1P5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_001499</name>
        <name evidence="3" type="ORF">DCAR_0101447</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B1P5"/>
    <dbReference type="STRING" id="79200.A0A162B1P5"/>
    <dbReference type="Gramene" id="KZN08843">
        <property type="protein sequence ID" value="KZN08843"/>
        <property type="gene ID" value="DCAR_001499"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF76">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN08843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG82284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="111" mass="11886" checksum="4FA30F09F50F02CE" modified="2016-07-06" version="1">MRDAAPLSGVDSKTTVGGGVEDVYGEDSSTEELSMTPWTVSVASGYSLLRDPHHNKGLAFSEKERDSCYLRGLLPPVVVNQDLQVITGELRGTGAAFVVLPYLLFCVINVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162BA15</accession>
    <name>A0A162BA15_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_004479</name>
        <name evidence="9" type="ORF">DCAR_0104849</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162BA15"/>
    <dbReference type="Gramene" id="KZN11823">
        <property type="protein sequence ID" value="KZN11823"/>
        <property type="gene ID" value="DCAR_004479"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204640"/>
    <dbReference type="OMA" id="HISAMWL"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN11823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG85658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="63250" checksum="257777276B46417E" modified="2016-07-06" version="1">MEEQEPLLDSVDQKNAGGFRNMSFIIANASFEKAATFGLNANMILYLTNDYHLGMVSGANIVNLWTAAGNFLPVVGAFVADSSVGRYPLIAFGSIVGLMGTILLWLTTMIPRARPPFCSVSTAQCDPSTTFQVIVLCFSLGLSSIGVGGIRSASMAFGADQFVKGYNKEKSLAALESYFSWYYAATVLAITISLTFVVYIQEQLGWQVGFGIPAVFMLLGALLFFSASSFYIRSKDTSSLITGFFQVIVASYKNRNFTSDSDKTIVYHHKKESELIVPSAKLRFLNKACIVGDSKNYLTGKQIPGSWSLCTIDQVEELKAVLKVIPMWSTGVLMSVTVSQGSITLVQTMSMDRHITSSFEIPAASLSISFFISAILWILFYDRVIVPIASRLLGKPFYLTPKLKMGIGILLSILFMAVLAFIEYTRRGIVLKQKISDNQESAAKMSALWIIIPYCLIGIGEAMNTVGQYEFFYSEFPKSMSSIASTLRDLSLSVGGLAATYILTIVDQVSRRNGKPSWISSNIDQGHYDYYYLVIAGLCVINMFYYVLCSWAYGPCELSARKDFQQQDKIDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SV38</accession>
    <name>A0A164SV38_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Son of sevenless</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_023812</name>
        <name evidence="3" type="ORF">DCAR_0727318</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SV38"/>
    <dbReference type="STRING" id="79200.A0A164SV38"/>
    <dbReference type="Gramene" id="KZM86678">
        <property type="protein sequence ID" value="KZM86678"/>
        <property type="gene ID" value="DCAR_023812"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193798"/>
    <dbReference type="OMA" id="IVSTYLW"/>
    <dbReference type="OrthoDB" id="1926790at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33133">
        <property type="entry name" value="OS08G0107100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM86678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39538" checksum="515DD74484126CDC" modified="2016-07-06" version="1">MEVSSGPSPRLNSISINNDTHADLDLQSHKDFDHHHHFASKSALEILRETVRILRYNLSGFMAITGVLICPVSALLLSNFLVDHRLVKRITIRLLLVVKSSGLPLRPFVRQSCQKFSEMAISSTMCFPLFITLLLMSKAAVVYSVDCTYARRMFDANKCYVIVTKIWKRIVSTYLWACTVMVGCLMLFLVFLVTVSSLLSLIGFPPDFNVYFAILIGLFFSIFLANAIIICNIATVISVLEDVAGPQALMRSSILIRGQTQVGLLIFLGSTIGMSFVEGLFEHRVKTLSYGDGSSRIWEGPLLVLMYSFVVLIDIMMSTVFYFSCKSYSLESSHEECQPVLEARNISPAAPEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TF97</accession>
    <name>A0A164TF97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">adenylate dimethylallyltransferase (ADP/ATP-dependent)</fullName>
            <ecNumber evidence="10">2.5.1.112</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0727877</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in cytokinin biosynthesis. Catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>dimethylallyl diphosphate + ADP = N(6)-(dimethylallyl)adenosine 5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36327"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73533"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dimethylallyl diphosphate + ATP = N(6)-(dimethylallyl)adenosine 5'-triphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36331"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73532"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.112" evidence="10"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TF97"/>
    <dbReference type="Gramene" id="KZM87445">
        <property type="protein sequence ID" value="KZM87445"/>
        <property type="gene ID" value="DCAR_024579"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195679"/>
    <dbReference type="OMA" id="DFCRHAT"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009824">
        <property type="term" value="F:AMP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052622">
        <property type="term" value="F:ATP/ADP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.890:FF:000002">
        <property type="entry name" value="Adenylate isopentenyltransferase 5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.890">
        <property type="entry name" value="Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF74">
        <property type="entry name" value="ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH08436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="331" mass="36976" checksum="06A92A44EAC18B06" modified="2016-07-06" version="1">MRISFSACKQAPPQPLVNFRGGVVSMDPFMPWRRKDKVVILMGATGTGKSRLSIDLATSFPAEIINSDKIQVYEGLDIVTNKVTEEECRGVPHHLLGMIRSDADFTANDFRHHATIAVESIVSRDRLPIIAGGSNSFIKALVNDEFKSRYACCFLWVDVSFPVLHSYVSDRVDKMIESGLIDEVRQFFNPDSDDYTRGVRRAIGLPEMDEFLRAEARVDDETRDALLEMAIYKIKGNTSLLALKQLRNIRRLRKQMEWDMHRLDATEAFLKSGSESHEAWERRVGRPSRGIVGNFLCEEDSALSTSFIKMKASSLVGAAAVSTAVVAGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TTM9</accession>
    <name>A0A164TTM9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Casein kinase II subunit beta</fullName>
            <shortName evidence="5">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0728748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TTM9"/>
    <dbReference type="Gramene" id="KZM87941">
        <property type="protein sequence ID" value="KZM87941"/>
        <property type="gene ID" value="DCAR_025042"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196777"/>
    <dbReference type="OMA" id="DADFGRC"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000003">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH09292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="289" mass="32612" checksum="8CA23F9F6524229B" modified="2016-07-06" version="1">MIRDRGGGSSKSEVVGGALDRKRINDALDKHLEKASANSRLLNLKERERLSIPSTSAAGSSKSQYDNRASVNLNLKNKCADEESESDSEESDVSGSDGEDTSWISWFCNLRGNEFFCEVDDDYIQDDFNLCGLSSQVPYYDYALDLILDVESSHGDMFTEEQNELIESAAEMLYGLIHVRYILTSKGMAAMLEKYKNYDFGRCPRVYCCGQPCLPVGQSDIPRSSTVKIYCPKCEDIYYPRSKYQGNIDGAYFGTTFPHLFLMTYGHLKPQKANQSYVQRVFGFKLHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WLF6</accession>
    <name>A0A164WLF6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0625662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WLF6"/>
    <dbReference type="Gramene" id="KZM91943">
        <property type="protein sequence ID" value="KZM91943"/>
        <property type="gene ID" value="DCAR_020692"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224838"/>
    <dbReference type="OMA" id="KWICIGI"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000003">
        <property type="entry name" value="Qa-SNARE, Sso1/Syntaxin1-type, SYP12A-group"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000008">
        <property type="entry name" value="Syntaxin 132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF80">
        <property type="entry name" value="SYNTAXIN-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH06239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="298" mass="33474" checksum="FE7206FF3A2667FB" modified="2016-07-06" version="1">MNNLFTSSFSADDENELNPSHVIEMTPTSGDNLESFFKDVEAIKLQLQSLDAHYTDLKSCHEQSKTLHNAQAVKNLRHKMDSHVSSSLKIAKQVKSNLQALEKSNAASRKLPNSGPGTSSDRTRTSVISGLSKKLQTSMSSFSSLRETIASEYRDTVKRRYFTVTGENADETVLEDLISTGESETFLQKAIQVQGRGQVMDTISEIQERHDAVRTIEKNLMELHQVFLDMSVLVESQGEQLDNIESHVERASSYVRGGTRQLEVAKKTQRNTRKWTCYAIILLLIIIAAIVLSIRPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WNJ9</accession>
    <name>A0A164WNJ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein kinase and PP2C-like domain-containing protein</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0625756</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 25">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 25">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the C-terminal section; belongs to the PP2C family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WNJ9"/>
    <dbReference type="Gramene" id="KZM92033">
        <property type="protein sequence ID" value="KZM92033"/>
        <property type="gene ID" value="DCAR_020602"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227430"/>
    <dbReference type="OMA" id="HVEEWNP"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019852">
        <property type="term" value="P:L-ascorbic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000035">
        <property type="entry name" value="Leucine rich repeat protein phosphatase 2c domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000683">
        <property type="entry name" value="Protein kinase and PP2C-like domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00072432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOH06331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="654" mass="72777" checksum="A596C92AC1162A7A" modified="2016-07-06" version="1">MGWEEDNTCFRGCCRSQTIPLHLPSSSYILSAPIARGAESIVYEATLNGQRVAVKKPILSTSQDIDRFHKELQLLCKLDHPGIARLVAANGKPPNYMFFFQFYESGSLADKIHVDEWCPSIDQALLITLQLANALKYLHDHGIVHRDVKPANILLDGNLNPHLADFGLAEYIRDIKQVSTENWRSSGKPTGGFHKKNMVGTLIYMAPEILRKELHSEKSDVYSFGISINELFTGVVPYTDLRAEAQAHTVLEMNYTEQQLTAAVVSEGLRPVLAGNEPGAPQRIVSLIERCWEANPHNRPSFDDIIIVLDSILEKSIIRATEEEKVPPETSLASPSPETTILRSYQQSINWFSQGERFMRSASADSCEGIWRLDSSNDSMLYHPILSWGSFATTGRRETMEDTHFLMPYLCNNKDIYMFGIFDGHRGAAAAEFSARALPGLLQTLGSTNSPDVALVKAFMEADLAFRNELDFRRKSKELKQKDWHPGCTAVVALIVRDKLYVANAGDCRTILCRAGHSYALSRDHVASIPEERERVISSGGEVKWQVDTWRVGPAALQVTRSIGDDDLKPAVTAEPEITETLLSAEDEYLVMASDGLWDVMSNAEVVSLIKDTVKEPGMCSKRLATEAAARGSKDNITVIVIFLQPVSTAERIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WSV4</accession>
    <name>A0A164WSV4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BolA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_020437</name>
        <name evidence="3" type="ORF">DCAR_0625964</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WSV4"/>
    <dbReference type="STRING" id="79200.A0A164WSV4"/>
    <dbReference type="Gramene" id="KZM92198">
        <property type="protein sequence ID" value="KZM92198"/>
        <property type="gene ID" value="DCAR_020437"/>
    </dbReference>
    <dbReference type="OMA" id="CIRATTQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF4">
        <property type="entry name" value="PROTEIN BOLA4, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM92198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH06536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17508" checksum="12F46A8F58A58355" modified="2016-07-06" version="1">MAARTLIIRPQMTSLCAFRPRLLSSLAFSSSSPLKYSPPELTIRRNYQSINTVSRRSFCIRATTQVNDPGSIDSPLMQSMKQKIQEHLNAESVSVKDAYGDGRHVSIDVIASTFEGQSAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPTEAESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XV23</accession>
    <name>A0A164XV23_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0519286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XV23"/>
    <dbReference type="Gramene" id="KZM93617">
        <property type="protein sequence ID" value="KZM93617"/>
        <property type="gene ID" value="DCAR_016862"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="90" mass="9190" checksum="6988885DB2F79278" modified="2016-07-06" version="1">MGVARGRGGAKGMGDGGDRGRGWASGRGDGGDRGRGGARGRGNGNRNGRADGGDGDNDGGDDFDYFLDDDVAMLIESLQRKTCKVIITKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZS85</accession>
    <name>A0A164ZS85_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small ribosomal subunit protein bS20c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">30S ribosomal protein S20, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_019570</name>
        <name evidence="13" type="ORF">DCAR_0522388</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZS85"/>
    <dbReference type="STRING" id="79200.A0A164ZS85"/>
    <dbReference type="Gramene" id="KZM96328">
        <property type="protein sequence ID" value="KZM96328"/>
        <property type="gene ID" value="DCAR_019570"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219978"/>
    <dbReference type="OMA" id="IYHKAHK"/>
    <dbReference type="OrthoDB" id="4825at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.110:FF:000003">
        <property type="entry name" value="30S ribosomal protein S20, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="entry name" value="Ribosomal protein S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_bS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_bS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_bS20_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="Ribosomal protein S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM96328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH02998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="186" mass="20652" checksum="B1EC73887EFA2158" modified="2016-07-06" version="1">MAVAAQCLSSSCWTISSKIQSLSLNTTKTHPNSCASFRPLGFSSNTSFNAFSTGFVSIRPVKRQTGQLIVCEAAGPTKKADSAAKRARQSEKRRIYHKAHKSEVRTRMKKVLESLEVLRKKTDAQAEEVITIEKLIAEAYSVIDKAVKVGTLHRNTGARRKSRLARRKKAVEIHHGWYTPSPELTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WWZ7</accession>
    <name>A0A165WWZ7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0415940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WWZ7"/>
    <dbReference type="Gramene" id="KZM97743">
        <property type="protein sequence ID" value="KZM97743"/>
        <property type="gene ID" value="DCAR_014895"/>
    </dbReference>
    <dbReference type="OMA" id="FITGEHD"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13920">
        <property type="entry name" value="Stellacyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000034">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF494">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043937984" evidence="6">
        <location>
            <begin position="29"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG96604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="20009" checksum="49C8B186DB1EB1D5" modified="2016-07-06" version="1">MASGRVNVVAFFVVMVIGAAMQMESTDAQKTHDVNGPAGWIIPSSPNVYSTWAASQTFAVGDTLVFNFTTGAHTAAQVTKAAYDACTITNPIAVWQTGPSSVKLNSSGPHYYICTIPSHCSLGQKVAITVGAAATSPASAPSPAATPTEAPAPGSTATPSDAPGSTTTPSAETPGNSASFAAAAWPLTVLSAAAMALFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XDV0</accession>
    <name>A0A165XDV0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_014572</name>
        <name evidence="4" type="ORF">DCAR_0416344</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XDV0"/>
    <dbReference type="STRING" id="79200.A0A165XDV0"/>
    <dbReference type="Gramene" id="KZM98066">
        <property type="protein sequence ID" value="KZM98066"/>
        <property type="gene ID" value="DCAR_014572"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218386"/>
    <dbReference type="OMA" id="CQVNESE"/>
    <dbReference type="OrthoDB" id="1908535at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044679">
        <property type="entry name" value="PWWP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33697:SF2">
        <property type="entry name" value="T17B22.17 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33697">
        <property type="entry name" value="T17B22.17 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM98066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="775" mass="85386" checksum="3AFECAE5E2439BEF" modified="2016-07-06" version="1">MGSSDTETSSGVVDSTVGAIVWVRRRNGSWWPGKILGADELSASHLMSPRSGTPVKLLGREDASVDWYNLEKSKRVKAFRCGEFDDCIDKAEASQGMPPKKREKYARREDAIIHALELEKHFLEKQYGKLGSSNYKSNIKKELATSAESMRHDNGKLTEPKSEQPTGGLDLPHGGIISPHLQIQEVREAKQLSGDDDKADVLPRMRGLEDIGLRTTTKSMIIPPIDSTGQLKSPLGDGSHAPFNGDATENTSHVNDKISLEKRKRSNEGFDEESLVKRRDKRRPLVQVLESSAKLPAPSSLKPEGGIVSTYIQVEEQTGGEGSAKRSKCVYQTKSSNSTDNTETHSEHLEVSASKLEVNSNPPPALGKDSTSGSTEDTETDSSETDSVESDSDEEMDEISDESVEFMPKFMLKSELQEEDDSTSTEEPDDPTTPSDMSHPSNDDVSASAGVSKWQLKGKRNTRALTKRYVDLSDGKVLRGSNQGPKIKEKGSYADLMDSFDKKYGTRIGGYGSRGVDGISRGITSWEDLAWNDQPASKGYWGDFIEYTDPVFSGRRFGDRRKCMLIEVDLKVQSNYQREHVPMISLMSKLNGQAIVGHPIQIEALEYGSTEGLMTAADELNQDNDTSLPPVWRTARRTANCRVPRPRASTVDADEGAEHLQHVYQDRKNSLNKSNGGNFSNRQSLARKSSTHGSRPTDKRLLRKPAKKTSVASNQKTRTLSSIATQQKPNNILKPGSNSSLNGFIKPEIAPPAVACIPIKLVFSRLHEELAGRHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XIS1</accession>
    <name>A0A165XIS1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416499</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
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                <person name="Sanseverino W."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Y4P4</accession>
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    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:nectar secretion"/>
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        <property type="project" value="EnsemblPlants"/>
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    </dbReference>
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        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
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        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG97816.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="271" mass="30232" checksum="CB645FD2FDFADF5B" modified="2016-07-06" version="1">MAALSFELLAFIFGLLGNIISFLVFLAPLPTFYQIYTKKSSDGFQSIPYSVALFSAALLLYYAFLKTNAYMIVSINGIGCVIESIYLFLYVLYASKKSKVFTIGLVLIFNVGGLGVIVLFSLLLVEGPDRVTLVGWICAAINIAVFAAPLSIMKQVIQTKSVEFMPFTLSFFLTLCATMWFFYGFFVRDYYIALPNVLGFLLGFAQMMLYCIYKNAKKESVYELDELKAKNKGQSAEGKLYTPTKIHKADSINGKELELHQVVVSTAPDHN</sequence>
</entry>
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    <accession>A0A165YBQ9</accession>
    <name>A0A165YBQ9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">DUF1639 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_013684</name>
        <name evidence="3" type="ORF">DCAR_0417328</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="2">Leaf</tissue>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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        </location>
    </feature>
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            <end position="100"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM98954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG97987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25002" checksum="7CB4FD1AD7B6C1B7" modified="2016-07-06" version="1">MEKGEKNERTACKLAEADLFLKWGNRKRLRCVRVRDRPSHLPSSFPDGRLRRRITSRFLTFPDTKHTFTQQSTRLTRNSEAATHRSEKRKLSPEKDDKHYSTRGSGLGLDEKGENGKIVAGENGGEESKSKGAVWPKLYISLSSKEKEEDFMAMKGCKLPQRPKKRAKMIQRTLLLVSPGAWLTDLCQERYEVREKKTTKKRARGGGLKAMGSFESDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YFY1</accession>
    <name>A0A165YFY1_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">endo-polygalacturonase</fullName>
            <ecNumber evidence="3">3.2.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Pectinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0417424</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.</text>
            <dbReference type="EC" id="3.2.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.15" evidence="3"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YFY1"/>
    <dbReference type="Gramene" id="KZM99043">
        <property type="protein sequence ID" value="KZM99043"/>
        <property type="gene ID" value="DCAR_013595"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218429"/>
    <dbReference type="OMA" id="FKPGANY"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009901">
        <property type="term" value="P:anther dehiscence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009830">
        <property type="term" value="P:cell wall modification involved in abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010047">
        <property type="term" value="P:fruit dehiscence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000028">
        <property type="entry name" value="Polygalacturonase QRT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG98083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="464" mass="50411" checksum="E0017AA6E014E9FF" modified="2016-07-06" version="1">MAPRSHIFHLLIIIVLVYSMISPCCSLEDEVQPNINDIHLFSDPDRETGFDSRAYPSPFLSSDGVSSDMAEVIEGGISSYLEKLAAESAAKIVSVKSYGAKGDGESDDTKAFKKAWDEACSSGAVFEVPENKKYLVKQIRFEGPCKSAMAVQIYGTILASDDRGDYKKDKRHWLIFDSVDDLVVEGGGVVDGNGKIWWKNSCKVHKSKPCKEAPTALTFYNMKNLTVKDLNIQNAQQIQVSFEKCEKVEASNLTVTAPGDSPNTDGVHITRTQNMQLSSSVIQTGDDCISIESGTQKLKITDITCGPGHGISIGSLGDGNSEAHVSDVVVDGAKISGTSNGVRIKTYQGGSGNASNIKFENIEMEDVKYPIIIDQNYCDKSKPCKKQKSAVLVKNVVYQNITGTSATDVAIKFDCSESHPCEGIVLRDVRLRLEKDEVAQALCNNVELQQIGTVSPSCPDIQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YV58</accession>
    <name>A0A165YV58_DAUCS</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM99285">
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        <property type="gene ID" value="DCAR_013353"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218492"/>
    <dbReference type="OMA" id="EHGFETW"/>
    <dbReference type="OrthoDB" id="10266805at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33987:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG98347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="458" mass="51757" checksum="B2484B4C33779D25" modified="2016-07-06" version="1">MNTKTPPFLRWLAVVVAVGALTISARITASNEDPVISRIAFGSCADQSSPQPIWDAINEYDPQVFIWLGDNIYGDNKRPFRVFGKERTIGPWKNVPRFYPVSEQEMRDKYSKAKNGPGYSRLRQTAKVIGTWDDHDYGLNDAGKEFSEKITNQRLLLDFLDEPQDSPRRKQAGVYTSYTFGPLGKKIKVILLDTRYHRDPLRSDGSILGASQWSWLEKELSGPASAITIIGSSVQVVSNISATTGPLFHLESWGRFPKERKRLFKLISDSKRDGVFFISGDVHFGEITRYDCGIGYPLYDVTSSGLTQAVEKVIPSPFQSFVRFVAWLTPTTMRVKNKSCRYKSCTYGQPNFGVVDVNWDAVPVSIKLEVRDARGMPVHSVNVSLSDLQARHKGSEYSMKAGEHRRHCSLEVTLPRIIRYRLAILFFCTLAVLLLLLAGLFYVVISAFIRCIRKHKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CDF0</accession>
    <name>A0A166CDF0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0314022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CDF0"/>
    <dbReference type="Gramene" id="KZN03620">
        <property type="protein sequence ID" value="KZN03620"/>
        <property type="gene ID" value="DCAR_012376"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG94725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="722" mass="80640" checksum="ADADB60C929E95C2" modified="2016-07-06" version="1">MSIKIVTSGGGLAGIILLGGALVTAALVSTFAYKRIQKSKKGDDDNCLIKCSKGGLSFLVRDCLSPQLTRDLRPSNGTEDDYVSRESMVLDDTKQVNGDKDEDIDILEKGRESPLCDESVTIMEDFPVQFADTSLLQKPEKLKKYDRFEKNSELKADEKILMANSDQVGVVCNGIKKNFDGNNKMDDLVEVVFKEHMPAEKCSENSEVIIEREEVLVKVPQSEQLGIDYDYQKRGDECDGILVEEREELVEAAVVNKIIRADNNPYQGGNECDADVGITIEKGEELVGGVAANGIQLSEKLDRDQGILCADGEIIQRNEDGTCDINKTSLLLVTDSSSLLKPASADLVEETLVIEDTKILEGDEADKISVQEDSIKVEDRVETQFVEPNEDCKIDRETTNDKWEGADEIGNDQQMQQCEEFQINEATKYSSDKSSPEMNSFQCDGLGVQEFSFPLLDSPPYCNFGNLIKDVYSQKALHVEDFNTMEKDKSANEVFSYQTMVERNPQSTNNDEMKSFNDGGENDVAKGEEILLTGLVDIGDGRKNDAEALEIIGTEDSELGNNQQPPLKEDNNYNDNVNEEEVSDSSEEEETSDSDCDEEDTGDEDEDISETSRGSSLASNAEAIWPVESVQELSRKIKPIQDNHNFMETEREDGHSNFNKHNLEEPEKNLKKEFSDKFTEEITTRRQATMFWFLSLLVPFLLLLWLLSSQLSSPDSSCCHMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CDN7</accession>
    <name>A0A166CDN7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CDN7"/>
    <dbReference type="Gramene" id="KZN03630">
        <property type="protein sequence ID" value="KZN03630"/>
        <property type="gene ID" value="DCAR_012386"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG94736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="21662" checksum="BF0F7376E8932965" modified="2016-07-06" version="1">MAEIHVEDAYTISSMRFLLTMKAFQAIIINSSTDPDIAELDVIIAQNPHLAAPLTRFRRSFLENKARRVEAIFSCNLVDAPYLEVNDVSSSEYVRYLREKCRWTGMACYDENLDGNLKNDVRKVLGDVPCRVKRVMEDGRIVLVPLPPEQMEQHSQDGSRKRYETRSKKSSVGCLEFGYTNRYKKCSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CYX6</accession>
    <name>A0A166CYX6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_005351</name>
        <name evidence="2" type="ORF">DCAR_0206002</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin-like modifier involved in autophagosomes formation. May mediate the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
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        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the ATG8 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG87217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KZN04891"/>
        <property type="gene ID" value="DCAR_005728"/>
    </dbReference>
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    <dbReference type="OMA" id="EMFGCFL"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000235">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Atg8-like"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
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    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOG87217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="124" mass="14411" checksum="4F077AB2285380FD" modified="2016-07-06" version="1">MGKKTIAFKDEFTFEERCQESQDIIAKYPSRIPVVAERYSKTDLPDMEKKKYLVPRDMSVGQFIHILGGRLRLDPGKALFVFVENTLPQTSSLMETVYDSYKDEDGFLYMCYSSEKTFGYGYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YAC8</accession>
    <name>A0A175YAC8_DAUCS</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0830944</name>
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    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM80499">
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        <property type="gene ID" value="DCAR_032247"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="64" mass="7420" checksum="7BA1F4A09AA4B005" modified="2016-09-07" version="1">MENSINDDIMISNLIYNLSRDDEFSHLHDSEIETNYEDIDDDGDSDSTNTDNDEEEEEYMPTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YBH3</accession>
    <name>A0A175YBH3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBH3"/>
    <dbReference type="Gramene" id="KZM80949">
        <property type="protein sequence ID" value="KZM80949"/>
        <property type="gene ID" value="DCAR_031435"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192541"/>
    <dbReference type="OMA" id="CANRNDY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF9">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG86540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="39334" checksum="C8E46F64EA1E0D63" modified="2016-09-07" version="1">MALAAKSWIIGRIITLLFYLKLVTFIDAAPQVPCYFIFGDSLSDCGNNNKLQSLAKVNSPPYGVDYPGGVTGRFTNGRTFVDFLAEFLGFDHHMPGFATVEGNAVLQGVNYASSGSGILNETGAIVGERFSLLQQIDNHKSIITRIKQIQGNNDQTTNQYLNKCMYTVNIGSNDYEGNYLMVPAANRTTPPDKWAAQLITAYSDTLKTLHGLGARKVAIWGLGQLGCEPSQVLEAKPKTPSGCVDDINKMISLFDNSLPKLVDDLSKTLPEAHFVYVNSSNISPGNDAAALGVKVTLEPCCKVGKTGGCIPNSAPCANRNDYSYFDLYHPSERSNLHGSLKAYNSTEPLDVYPMNLQKLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDD6</accession>
    <name>A0A175YDD6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_029239</name>
        <name evidence="13" type="ORF">DCAR_0933663</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Centromere</location>
            <location evidence="8">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDD6"/>
    <dbReference type="STRING" id="79200.A0A175YDD6"/>
    <dbReference type="Gramene" id="KZM81626">
        <property type="protein sequence ID" value="KZM81626"/>
        <property type="gene ID" value="DCAR_029239"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201094"/>
    <dbReference type="OMA" id="PSHKFQK"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055307">
        <property type="entry name" value="NDC80_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46681">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46681:SF1">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 8" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="11">
        <location>
            <begin position="37"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="209"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="441"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM81626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH14147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="570" mass="64965" checksum="AD1BFBAAAE07D05B" modified="2016-09-07" version="1">MRPAGARRQKDSIAGDHRKPPPPTPTSFDPFQYNFSNRDSDASLCSSRPSIASTTRNPSVPISDKSFQLSALRTVNNYLSLHSATFSLKPPLPSAKDIIETLKLILKNLDFPMSGGNKFEDELFGVLKYLGCPVKVNRSALKAPGTPHSWPSLLGVMHWLVQIGNYDEVTRGNGLEYEENGMFDYALRGYFHYISGDDERMESEDQMFLEKLTNERNVVEEGARGLAERVRELEGKLEGLKNGPSEKEVLEEQRKLLEEDVKKFYTMIEQLDGHIVNVQKSLEEKEKELEAKVEERKRICEENEELKSRVEEQGFNARDAERMKRELQAVERNTGDAEAARSAWEEKSWELDSEIGKKFKELESLMIECNQAIRKLKLGNGFQYELNAKVSTPAEVLGIDYKLKLKPVLASLAEETKKSSMVKLEEQISLQKLFVEAANKIEAKRNHIAALQFHIDEVENQLNLLRDETQEHISRCMMEAKKLVEDVKAEEQNMDIVEKEATELLRTSQMKCQEVNAQSEEEVQMCARELLALIDSVSKFKESMTSTISEMKEDYLKTVSVISEIHKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKF7</accession>
    <name>A0A175YKF7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase III subunit RPC6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_030901</name>
        <name evidence="7" type="ORF">DCAR_0935858</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM83332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKF7"/>
    <dbReference type="STRING" id="79200.A0A175YKF7"/>
    <dbReference type="Gramene" id="KZM83332">
        <property type="protein sequence ID" value="KZM83332"/>
        <property type="gene ID" value="DCAR_030901"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200229"/>
    <dbReference type="OMA" id="FSCDIFN"/>
    <dbReference type="OrthoDB" id="613763at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM83332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH16307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26681" checksum="2F74740F98985EA7" modified="2016-09-07" version="1">MSRMQDTLALERRRGRQNLFTQSLGDADRNVLNIVRGKETRGISSGELKRVSNLPTSQLNKTIKALIASQLIKEVPNVRSTYGKHYMGVEYEPSTELTGGSWYANGKLDQDLINFFRGTCLQILQKKLKVATAEGVHSFFAKAKFYKGDVSREMIAEILRSLVLDNEILEVKSTGLAEYHSIPVGEVCYRYVIGGGTGEASRTGVMVSIPCGVCPQIRLCTPDGVISPTNCVYYTKWLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YN32</accession>
    <name>A0A175YN32_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_027811</name>
        <name evidence="12" type="ORF">DCAR_0831652</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YN32"/>
    <dbReference type="STRING" id="79200.A0A175YN32"/>
    <dbReference type="Gramene" id="KZM84767">
        <property type="protein sequence ID" value="KZM84767"/>
        <property type="gene ID" value="DCAR_027811"/>
    </dbReference>
    <dbReference type="OMA" id="CCNPLQQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044748" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM84767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH12153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="113" mass="12022" checksum="561CBD07D491FEA2" modified="2016-09-07" version="1">MSSSGKMKTLILGVVLVIALTAIVEAQHSCASTLVACVGSIDATTKPPSACCDSFEEVVAHQLECLCNLEYNTPDLLRSLEINVTQALQPPTLCGVPDNLCQGAFLYLCVFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YPY5</accession>
    <name>A0A175YPY5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0831791</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPY5"/>
    <dbReference type="Gramene" id="KZM84882">
        <property type="protein sequence ID" value="KZM84882"/>
        <property type="gene ID" value="DCAR_027696"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199935"/>
    <dbReference type="OMA" id="YTAFICM"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000530">
        <property type="entry name" value="ABC transporter G family member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF11">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="744" mass="82209" checksum="39DBCFA8257B3873" modified="2016-09-07" version="1">MSRVVDENSSPDDQQDIERFDSASPTLGQLLKCVGDVRKEVTGDETPVHQAVDMSSAMGTEPRSVPFVLSFSNLTYSVKVRRKLTFSNLCGGGKRSVAGSAAPAEPFATENMFSRSKTLLDNISGEARDGEVLAVLGASGSGKSTLIDALANRIAKGSLKGTVSLNGEQFESRLLKVISAYVMQDDLLFPMLTVEETLMYAAEFRLPRALSKSKKQLRVQTLIDQLGLRNAANTVIGDEGHRGVSGGERRRVSIGTDIIHDPILLFLDEPTSGLDSTSAYMVVKVLQRIAKSGSIVIMSVHQPSYRILGLLDRLLFLSRGQTVYSGSPANLPLYFSDFGVPMPENENRTEFALDRIRELEATPGGTKSLVEFNKTWQNMKSSRRNTASYLNSPSPLHNLSLKEAISASISRGKLVSGAVSSDVTRASMVPKFANPIWNEIAVLSRRSITNSRRMPELFGIRVGTVMTTGFILASIFWNLDNTPKGIQERLGFFAFAMSTTFYTCADALPVFLQERYIFMRETAYNAYRRSSYVISHAMVVFPTLIFLAALFATLTFWAVGLDGGLSGFLYYFLIMLASFWAGSSFVTFLSGVVPSVMLGYTIVVAILAYFLLFSGFFITRNRIPDYWIWFHYISLIKYPFEAVLQNEFEDPNKCFVRGIQIFDNSPLGAVPDALKVKLLEGMSKTLGVPLSATTCLTTGADILKQQGVTDLSKWNCLFVTVAWGFLFRILFYFSLLLGSKNKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YRM7</accession>
    <name>A0A175YRM7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
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    </evidence>
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</entry>
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                <person name="Senalik D."/>
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        <source>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    </reference>
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    </dbReference>
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        </lineage>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
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        </reaction>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF93">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIF1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <end position="256"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG82070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="341" mass="38244" checksum="819E8091C4112280" modified="2024-05-29" version="1">MQNKLAFECLDRTLRDIMKSVSPDRYNKLFGGVTVLLGGDFRQILPVIRKGSRAEIVAASISRSYLWSECRLFVLQENMRLRRGKNAAEQRRIKEFTNWVLDVGDGIIPHLDGGFSEDNTGNVQIPSEYCIQSESSDVNVLIDMVFPDLHERFTDVDYLRERAILTPTNKVVDHLNNLIFDKIGGESHLYQSADSVQCPVGCHENLTSAFPVEYLNSFKLPGMPPHKLILKEGVVVMLLRNLNQTVGLCNGTRLIVRKCLKHTLYVAVSRVTSPEGLICFIEKPEGGTGSLTMVENIQPLQIVNTPRGTVRLIKMIVGDGLYVFLPCSCLLCSICFVLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4E1</accession>
    <name>A0AAF0W4E1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0101959</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4E1"/>
    <dbReference type="KEGG" id="dcr:108206235"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF286">
        <property type="entry name" value="PROTEIN S-ACYLTRANSFERASE 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="159"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="391" mass="44777" checksum="C304BC2C639E1D00" modified="2024-05-29" version="1">MGVQWLLVCHGLVTLLVVVSFLCGRWPIFDGTFIQRIHIFLTFGAYDYFQRFVNYIFGTKGTNAILSIEYYCCDRPNPILQAIYLAIIGATYYFIVTSSFNYIPGHYIGGIHRYLSVVAVGVGVLLFLLTSFSDPGIVNNENFSRYVSVYPYDNAIYIEKECSTCKFTKPARSKHCSICDRCVARFDHHCGWMNNCIGERNTRYFVAFLIWHFILCVYGAVAIALILAGQLKESQVIYILTEFYGIDNSFGSLAPHVVQWFLGSYNTQILIMVFLAIVSLLLAGFFGYHANLCLTNTTTNETFKWHEYLSWKRKVNEARASAEALKSSLAELSQEKPPQSKWKTFFQRSPLKDTEPVKNNIYDRGYIRNVSEIIFPLSSRQLFKQYKSKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W735</accession>
    <name>A0AAF0W735_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102556</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W735"/>
    <dbReference type="KEGG" id="dcr:108221655"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="51"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG83381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="140" mass="15478" checksum="E4B01FAD2414FE2B" modified="2024-05-29" version="1">MGGLTLNQRDAEYEHKYKKLNQNCFISIFLQIPTCIAHVTIITESILEKPVVLFSKTSCCISHTVKSLLQDFGVSPVVYELDEIPRGRDIEAALAKLGHNPVVPAVFIGGKLVGGANEVMSLHLQLYLRPMLTRAGAMWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8C2</accession>
    <name>A0AAF0W8C2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0103850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a heterodimer with PSMG1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8C2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proteasome assembly chaperone 2"/>
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    </dbReference>
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        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
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        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG84666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="281" mass="31112" checksum="48BCD67559E7962D" modified="2024-05-29" version="1">MEFISEDGKTLDQQCSTLVLPALSIGNVGQLGVDLLISSTRAQRIGYLDDPNVLPCVGNDAYTPSLKPQLALSLEAYDSSSNSLTFVQQRSPVVKGMMLEYAKNLADFAAANGKKHVVVLSSLDFGRWQTIDMSSGLQIYYLSSANMDGTDDDCERLGWKRLQEYNPAQRRWKYLDDLAEGKMVQEDTSTFEDDMQDEDYLPSLPFAAMFSCFKAKGLKVTCLLCYCSEGDNMPDSFQLAEAASKLVGLRDTDIQGNEGGKWTIPFSWQSVYGPPADMSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W915</accession>
    <name>A0AAF0W915_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0104618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04640">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25301" checksum="362E79CCD281DE25" modified="2024-05-29" version="1">MKGAGGPEAEDNRWPPWLKPLLRETFFVQCKLHADSHKSECNMYCLDCINGPLCSLCLNHHRDHRAIQIRRSSYHDVIRVSEIQKYLDITAVQTYVINSAKVVFLNERPQPRPGKGVTNTCEACERSLLDSFRFCSLGCKIAGTSGHFVRKRMPEKKRKATVWDIEESYDSSGSSSHGHQRGNFGNLRSFSPSTPPPTVASFRTAKRRKGIPHRAPMGGLIIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBW2</accession>
    <name>A0AAF0WBW2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0205854</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WBW2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
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    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF385">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="4">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG86637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1050" mass="118739" checksum="0083E3F5190D66E1" modified="2024-05-29" version="1">MARVSQTPTSSVSTSIPTRWDVFLSFRGSDTRYKFTNHLYSALDRHGIRTFMDDPELRTGEDSKSYIVVFSEDYASSRWCLDELVEIYNSYETMKRLVVPVYYNIDPSDVRHQTGSFKHAFVKHQSRSGADIDKVKKWRFTPANVATFSGKTMSAKRSEADIVNEIVDIIHNEIGSDNLYVAKYPVGLDSRVKEITALFSSPPQGVTRIGIYGMGGVGKTTLAKALYNQLLLGSFTGICFLANVREVSGTFRGLESLQQKLINNVLKSKKKFEVPDVEEGIKFIRERLCSAKVLLLIDDIDNPRQYESFIGSFASGSVVITTTRDQEILEKIEVEPKFQYRVNELDDAESLTLFTRYAFGSAKSNDTLMALSKVILHLAGGLPLALIVFGAYLSTQSLLGWKSYIEKLQRNPDNTIQQNLIVSLDTLERDDPKLKKMFLDIACFFIGRKKEVVVTIMETYYSYVDHNIDILRKRCLLTTNDDGELRMHDLLRDMGREVARNNSPDEPGKHSRLWISKDIRDVLKNDKGTEAIEGIIHDNKFESYYAVWKESLNVETFKRMRNLRFLQLSCVHLTGSFEGALEDLRWFCWDLCPLERLPRGFHPEKLVILELTSCSIKMWEIEMVFENLKSLDLSYSMDLSSTPDFRKLPFLETLRLVACKSLKEVHISIGSLKRLVSLNLCNCVNLRSLQDSICNLRALKSLNISGCSSLEALPAELGNIKSLNELNAERLSVTNLPDSIGCLDKLVELRLSYNMNLDTLPDNICNLRLLGVLHISDCSRMKAFPLEFGKLESLKKLNAMELNISILPNSLGNLRQLVYLNIHSNYDVETLPDSICNLRALEVLKVGQCFCLKELPEGLGYLESLTRLDAQSLEISEIPSSIGSLSNLVVLILSCNTNLKTLPDTLCTLRSLEILDISKCEKLETLPDHLFKNTRLRQINARHSTMLRKFPGISQLSNLKHLDLTGCCNLLSIAELPPNLKVIRANGCKSLKSLPDLSNLKQLKRLNLRNCSALTEIQGLEELTSLKVLHLTGCDSSLLACIFTRHFFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WC22</accession>
    <name>A0AAF0WC22_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Plastidal glycolate/glycerate translocator 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0104576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WC22"/>
    <dbReference type="KEGG" id="dcr:108209625"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG85388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="54195" checksum="951876E5F7985E02" modified="2024-05-29" version="1">MATLSALPRISSFSLSNHPDSRFFTRKNPPKLLNIFAQNLHNRHNVTKSWTPFSVIDSNSKFLQVGHSARPVLVKSSDNGTSATSNGLQSVVGVLHLVVSLGIILAMDKYLKQAFVAAAIKFPSALFGMFCIFSVLVILDLTSPGLANGLMEFFEPALLFIQRWLPLFYVPSLVVLPIAVQDIPAASGLKIAFIIVGGWLASLAVAGYTAISVRKMVKTEMIPAEPMSKPSPFSTIELWAWSGIFVASFAGAYLYPTLLGTPARTCLPFLLASTVLGYIIGSGLPSGAKKVLHPIVCCALSADLAAYAFGYLSKSGIYPILGHYLTKVSSNPGAGDILMGFLGSVILSFAFSMFKQRKLVKRHAAEIFTSVIIATLFSLYSTAFMGRLVGLEPSLTISVLPRCITVALALSIVSLFEGANSSLTAAVVVVTGLVGANFVQVMLDKFRFDDPIARGIATASSAHGLGTAALSAKEPEALPFCAIAYALTGIFGSIFCSIPVIRQSLLAIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCR5</accession>
    <name>A0AAF0WCR5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Enoyl-[acyl-carrier-protein] reductase, mitochondrial</fullName>
            <ecNumber evidence="11">1.3.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">2-enoyl thioester reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0206775</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.104"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.104" evidence="11"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCR5"/>
    <dbReference type="KEGG" id="dcr:108208867"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000112">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000010">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="15">
        <location>
            <begin position="55"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG87547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="376" mass="40855" checksum="8D031C7B0A722479" modified="2024-05-29" version="1">MASSSFLKLLTLKPLHKPPSSSIIINHFSNMKFTFRSFSAAMSPPSTAVMYDQEGPPDSVTKMVELPPAEIKENDVCVKMLAAPINPSDINRIQGVYPVRPQVPAVGGYEGVGEVHSLGSAVKGLSPGDLVIPCPPSFGTWQTYVVKEQSVWHKVAKGTPVEYAATVFVNPLTALRMLEDFVDLNPGDSIVQNGATSIVGQCIIQLARIRGIRSINIIRDRAGSDEAKEKLKKLGADEVYTESQLDVKNVKSLLDNIPEPALGFNCVGGNAATLVMKFLRQRGTMVTYGGMSKKPVTVSTSAFIFKELSLRGFWLQNLVSSAKGEECKSMVDYLLGLARDGKLTYGMESTPFKDFHIGLDKSLGKLGSQPKQIIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDF0</accession>
    <name>A0AAF0WDF0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
            <ecNumber evidence="4">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0207140</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDF0"/>
    <dbReference type="KEGG" id="dcr:108206067"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000045">
        <property type="entry name" value="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000009">
        <property type="entry name" value="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="150"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG87907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="396" mass="43288" checksum="935B3F2BD36AF78D" modified="2024-05-29" version="1">MASTQFLCSSRQLYTSKTQPNCFRNGGLKTHLPFSFSTKKTHFKTAHFVTIKASAGKKQVEIVYNPDERLNKLADEVDANAGLSRLTLFSPCKINVFLRITSKRKDGFHDLASLFHVISLGDKIKFSLSPSKSTDRLSTNVPGVPLDDSNLIIKALNLYRKKTGSDKFFWIHLDKKVPTGAGLGGGSSNAATALWAANQFSGCIATEKELQEWSGEIGSDIPFFFSNGAAYCTGRGEVVQDLPAPIPSDIPMVLIKPPEACSTAEVYKRLQLDRTVSVDPLTLLEKISTSGISQDVCVNDLEIPAFEVLPSLKRLKQRVVAAGRGQYDAVFMSGSGSTIVGVGSPDPPQFVYDDEEYQDVFLSEASFITREPNQWYREPYASNAGADSTADFAQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WGA3</accession>
    <name>A0AAF0WGA3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit epsilon, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0208660</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WGA3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000002">
        <property type="entry name" value="ATP synthase subunit epsilon, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="10" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025364"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG89422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="74" mass="8263" checksum="96A6F40877438535" modified="2024-05-29" version="1">MASSGAAVPFWRAAGMTYISYSNICASMVRNCLKEPHKTESISREKVHYSVSKWVDGKPQKPRTNVYSASDTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name evidence="4" type="ORF">DCAR_0209038</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="306"/>
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        <location>
            <begin position="191"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89799.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="312" mass="36040" checksum="5EFEE458D79A0F62" modified="2024-05-29" version="1">MKTMRHLERVKSRISFTTDMWTSKNKKRGSWFAYVPCPHTAEALCEVLMETFLEWNVDSKLNTLTVDNCTTNDAMINLLLDKFGSNQIWLKNFFHIRCAAHIINLIVKEGLEYYYIKLFGERGPKMVERVKSSCYDLLTLYEGKMKKKKNDSESTLASKSSSISLSNRGVSDYEIFLSQEKKGKVSHVKSELDNYLEEKQLPRSEEFDILAYWKTNLKYPILQAIARDVLAVPVSTVASEAAFSTSGRVLNERRNRLTPKTLESLMCCQSWLTALEEGGSILYFWGCYIFGYIYVIIILIHLLLFLNRSSNY</sequence>
</entry>
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    <name>A0AAF0WJJ5_DAUCS</name>
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        <name evidence="6" type="ORF">DCAR_0310333</name>
    </gene>
    <organism evidence="6 7">
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        <lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="AGL218WP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    </feature>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <protein>
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            <fullName evidence="7">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="8" type="ORF">DCAR_0310773</name>
    </gene>
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        <lineage>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLW9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="7">
        <location>
            <begin position="78"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="462"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="798" mass="89936" checksum="5408046BAF5C0D21" modified="2024-05-29" version="1">MAGGSRAQLNKPHKTRFASKATRNVHRTSLKDKKISKPDRNVNKGARAARLQRNKMVREQKRAAVLKEKRAGSGSTSPPRVVVLFGLSASANLDSLAEDFVHLLSNGKTGTDLTVSSSEYKLRTTVLRAPHGDLLACMEMAKVADLIAFVASAHSSPEVESSSYYIDSFGAQCLSVFRALGLPSTVVLIRGLPTELKKRNELKKICASTLASEFPEDCKFYPADTKDDLHKFMWLFKEQRLSPPHWRNQRPYLMAQKVDCIATDSDAEKCTLLLTGYLRARSLSVNQLVHISGAGDYQLCKVDFLQDPCPLNLRKEADIMDSYELHSVQVISSLVPDPMKQEPLLVENLPDPLAGEQTWPTEAEMAEADKIHMERKLKKRTLPRGTSEYQAAWIIDDSDVDNSDVDNSNSDVDADDDMVLDEGNKSIHGQDMLETFDDDDNKTFVSLNDRESDQESEVDSVMMEGEMTKEQIEEEIRRIKEAHAEDEEYPDEVDTPLDVPARKRFAKYRGLKSFRTSSWDPKESLPPEYARIFTFDNFTRTQKHVLAKALEKEDGDKDECIPASSYVRLHIKEVPFSVASKLCDRSKVSPVIACGLLQHESKISVLHFSIKKHETYSAPIKAKEELIFHVGFRQFVTRPIFSSDSINSDKSKMERFLHAGRFSIASIYAPISFPPLPLVVLKGGDVAAPAVAAVGSLRSIDPDRIILKKIILTGYPQRVSKKKASVRYMFHNPEDVRWFKPVEVWTKCGHRGRIKEPVGTHGAMKCLLNGVLQQSDTVCMNLFKRTYPKWPQHWFPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WP12</accession>
    <name>A0AAF0WP12_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Heat shock 70 kDa protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0311103</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the heat shock protein 70 (TC 1.A.33) family. HSP110/SSE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WP12"/>
    <dbReference type="KEGG" id="dcr:108213531"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042122210" description="Heat shock 70 kDa protein 17" evidence="9">
        <location>
            <begin position="26"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="838"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="844"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="863"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="887"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG91848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="902" mass="100957" checksum="8A019599CBE60363" modified="2024-05-29" version="1">MAKISLGLALYVLLLSLLCFHSVVIDSAVSSIDLGSESLNVAVVNLKPGHLPISIAINEMSKRKSPALVAFHSGNRLIGEEAAGLVARYPNKVLSSFRDLIGKSHEFAKNALERMYLPFEIVDDPERNVVAINAEGDGDSYVVYSVEELVAMVLGFASRLAEFHAKVEVKDVVISVPPYFGQVERKGIMQAAELAGINVLALINEHSGAALQYGIDKDFSNESRNVVFYDMGSGSTYAALVHFSAYKTKEFGKTVSVNQFQVKDVRWDPKLGGQDMELRLVEHFADEFNKQVDSGIDVRKSPKSMAKLKKQVKRTKEILSANTMAPISVESIYEDRDFKSSITREKFEELCEDLWERSLVPLKEVLKHSGLQANDLNAVELIGGATRVPKLQAKLQEFLGRKVLDKHLDADEAIVLGASLHAANLSDGIKLNRKIGMIDGSMYGYVFELTGSGLSKDENTRQLLVPRLKKLPSKMFRYIAHNKDFEVSLAYDTEDLLPPGVLSHTFAHYVVSGLTDASEKYSTRNLSSPIKASLHFSLSRSGILSLDRADAVIEVSEWVEVPKKNLTMENSTFESPNMSAEASPKNASEEIDESLLVTGELLSNSSEIVNDQNSTDIVTEKKFKKRTFRVPLKIIEKALVPGKLFSRESLAKAKSRLERLDKKDAERRRTEELKNDLEGYIYATKEKLDSDEFEKVSSSQERQSFIEKLEQVQDWLYNDGENASAAEFQQRLDSLKAIGDPISFRYRELTARPEATQLARRYFDELQQIVQEWETKKSWLPKERIDEVLTDTEKVKNWLVEKEAEQKRTPVSSKPAFTSEELYEKVFDLQDKVASVNRIPKPKPKVEKAVKNETEGIGNKTDSSNSSSQYSSGSQGDETTNDSEVLAEERSNANESNSRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPL6</accession>
    <name>A0AAF0WPL6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate--ammonia ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0311408</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPL6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF110">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG92148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="186" mass="21168" checksum="9CB95D321332F9D0" modified="2024-05-29" version="1">MRLTLITLNFITSLSGPVDDPIKLPKWNYLDQVLVKHQGMIDLFRRANHILVYTPADEPILTNNRCNAAKIFSHPDLQLMFLGMLFKFSPIGWPTEGYPDRRLAYIYLYGPYYCGIGADKAFGRDIVDAHYKASLHSGINISSINGEVMPGQKGFSIGQTLHPFTADFTNLLHNYKKMEETIFRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRL6</accession>
    <name>A0AAF0WRL6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0313380</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRL6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000877">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="48"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="288"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="524"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="534"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG94089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="611" mass="68402" checksum="2AE7443C2DF71EF3" modified="2024-05-29" version="1">MATESYNPTGALTKTRFSDLQPRLSEPVLQALSQSGFDFCTPVQAATIPLLCSYKDVAVDAATGSGKTLAFVVPLVEILRRASTPPKPHQVLGIIISPTRELSSQIFHVAQPFISTLSNVKPILLVGGAEVKSDMKKIEDEGANLLIGTPGRLYDIMDRMDILDFRSLEILILDEADRLLDMGFQKQITSVISRLPKLRRTGLFSATQTEAVEELSKAGLRNSVRVEVRAETKSINSAQSSLQSVSSKTPSGLLLEACTFLSRIITLQLIYILNCNYLECEADKKSSQLVDLLVKNKSKKIIIYFMTCACVDYWGVVLPRLSALKGRSLIPLHGQMKQAAREKALASFTSLSSGILLCTDVAARGLDIPGVDCVIQYDPPQDPDVFIHRVGRTARLGRQGSSIVFLSPKEEAYIEFQRIRRVPLEERKCCDEAFDVVPEIRTAAKKDRDVMEKGLRAFVSYVRAYKEHHCSYIFRFKELEIGRLGMGFGLLKLPSIPDVKHHSISTEGFIPEQNVNLEDIKFKDKSRQKQRNKNLQEKKAKQELEPKRQKPKLSLKNATTVNRKKTAQQRRAVQTAEDDDELTRDYRLLKKLKKGAIDENEFAMLTGTEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WS23</accession>
    <name>A0AAF0WS23_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0314286</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WS23"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF57">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042069328" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="14">
        <location>
            <begin position="22"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="778"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="15">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="16">
        <location>
            <begin position="230"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG94985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="827" mass="92246" checksum="91CBA7D16F2E3DB1" modified="2024-05-29" version="1">MPYCHLLILVFFLLLLPSSLATRKEGEALVKWKDSLSPSSFLESWSSTNLNNLFNWTGITCNSEALVSEINLSRKQLEGTLAEFRFNLFPSLNNFTISGNSFTGPIPPEIGTLTELQHLDFSFNSLNGFIPYQVSHLQKLRTLDLSYNLLEAPNWSKFSPIPFLTTLNVRFNNLVSEFPEFISNCHNLAKLDLSHNFLTGDMVRESVFNNLQDLKFLSIMENSFEGPFPPSILNLSRIRHLELSSNNFSGSIPQNIGMLHDLKYLSLFANSFVGSIPSSIGLLRELQILDLSYNHLNSNIPREMGLLTNLSELYLDMNNLTGNIPSELGLLTKLNLLVLLSNNLTGNVPSEIGNLKLLTQLLLGSNQLTGPVVKSVFNLSNLEVLGLPNNKFTGNIPGDFWKNLKSLLYMDCRDNLFSGTILGLNTNLNLTYIDLSEFPSTICNVTSIEVLDLSHNGLSGALPHCVGKLANNLHTMVLGNNKFQGIIPTTFSNTCHLEVLNMQNNHFEGMVPQALANCKELKILDMGNNHISGTVPSWLSTLQNLEVLVLRSNRLHGKVSASSTKHPFPKLRIVDLSNNEFTGNLPIQYFYYMKPTRKGYDYNNSKEDFQYFYQASVPLRVKGTEYEVKEILYIYTAIDLSCNKFQGKVPKVIGDLSSLALLNLSHNSLTGPIPSVLGNMETLQSLDLSSNQLSGVIPNQLTSLTFFAVLNLSENHLTGEIPLKGQFSTFGNDSYLGNSALCGLPLTKKCMHAVSPPQEVGNDGDDEDDVHNGLNWELILMGYGCGLICGLSSGYIAFKMGKPRWFVRYIELLQLKLIRTRMKKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTS1</accession>
    <name>A0AAF0WTS1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0415229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTS1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF5">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="123" mass="13511" checksum="CB627FC56C9F385D" modified="2024-05-29" version="1">MNSPLPFFFPFLDEEPTVAGFEVPRSPDASYNNVYPGNADEGPEPPVVPPHLQHTLLNHPSGRGGDAPTSVPLPQNVVLNHLYLENREAPRSVVALGITHRFRAKYVTVVLYKPVQRNGSTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWF5</accession>
    <name>A0AAF0WWF5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0415507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WWF5"/>
    <dbReference type="KEGG" id="dcr:108215780"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000037">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000088">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045:SF20">
        <property type="entry name" value="UDP-RHAMNOSE:RHAMNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG96176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="52995" checksum="B4BCD22D9ECBE908" modified="2024-05-29" version="1">MASVEKLHIVMFPWLAFGHISPYLKLAKLMAAKGHKISFISTPKNIDSLPKIPQNLAPLINLVKIPLPLVPNLPENAESTTEVPFHKVKYLKLAYDLLQEPITNFLESSTADWILCDYVSYWLRPIASRLRIRWCHYSVYTASFMGFLGPPSNMIKGDDYRVRPEDFMVKPEWVHFDTNVAMSLYQILALAPELEADEDTKNVSEAYRAGRSLEYCDMVAIRSSVEFEGDWLKLLQDMYEKPVIPVGLLPHVEESTEADNQDWIEIKDWLDKQAKGSVLLVAFGSEAKLTQAQTTELALGLELTGVPFFWAMKKQRGLSDTEAVELPEGFQDRTRGRGMIYTAWVPQIKILNHESVSALLNSSGYSSVVEAMQFGKALILLPCVYDQGIIAKHLEEKKLGFQIPRNESDGGFTRESVAESVNLVMLDEEGKIYRDKVKDMQAILCDMDKQNGYVDNLLNHLQNHKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYF1</accession>
    <name>A0AAF0WYF1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L10e/L16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0416546</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYF1"/>
    <dbReference type="KEGG" id="dcr:108215766"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG97206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25144" checksum="656F9D77169C799D" modified="2024-05-29" version="1">MGRRPARCYRQIKNKPYPKSRYCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMTKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPLGTCARVSIGQVLLSVRCKDTNSPHAQEALRRAKFKFPGRQKIIISRKWGFTKFNRTDYIKWKAENRILPDGVNAKLLGCHGPLARRQPGRAFIDAVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3B0</accession>
    <name>A0AAF0X3B0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0518961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3B0"/>
    <dbReference type="KEGG" id="dcr:108221217"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="362" mass="40302" checksum="553758C4EBCC4CEC" modified="2024-05-29" version="1">MKVVDMEGVNGATILVDSTGCSVWEDDGGEDAGGGSDATGDGGGGEGGGRLDSLVVDTVEANMEGHEVIRCEGGGGLGWRRSCRRFLLCAEDASILRAVNFKFEMEFTKVYHFLCVKGLLVKCSEAGNLAARHMLGKVILLSSTHLLFSETKSKILDVSNFDIPTVNCWIRDAYVPLHNMKVCSFMSYFFKVSSPKRLVHHQVVKFFLLNGSHHDFVEMAPFLKYYVMFFTKFTGGHCRLINSIELLARTASRVRTMEKLTKTKESLNGYIRGLGEFIEMTTEEIADRIPEDTSGKVMDYLHLQNFYLQKCTTDGVDQLVAMTDANFVEALRDDVLNLLYDANIELSKIRAATVGQFENKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3P0</accession>
    <name>A0AAF0X3P0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0519946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="2">Leaf</tissue>
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                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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            <dbReference type="EMBL" id="WOH00887.1"/>
        </source>
    </evidence>
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    </evidence>
    <sequence length="67" mass="7660" checksum="2DE09E522FA76584" modified="2024-05-29" version="1">MSFCYCGKLAADRVAWTDANAGGCRFFQWVDGPLCSRAQIVIPDLLRNMRGMESNYERQIVEQKKLT</sequence>
</entry>
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        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
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    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
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    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8Z3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF713">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="250"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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</entry>
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    <name>A0AAF0XBA8_DAUCS</name>
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            <fullName evidence="5">Glycine-rich protein</fullName>
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        <name evidence="3" type="ORF">DCAR_0522560</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <location>
            <begin position="1107"/>
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        </location>
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        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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</entry>
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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            <dbReference type="EMBL" id="WOH06941.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <sequence length="78" mass="8041" checksum="73F2E35B9B3EC17A" modified="2024-05-29" version="1">MTMRSSCLVLVLMILLTNAQFSVVECRAFPSNVAEEGAATGAAMLVVSPRNSGGGVSSAVQSLMFKLASGPSKRGRGH</sequence>
</entry>
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    <accession>A0AAF0XF00</accession>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS09G0482800 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="15833" checksum="3632704B34F0DB44" modified="2024-05-29" version="1">MSVHVLDGATIVSFVEDDEAFTGSVHERFVLLDTDHNGVLSYAEMLKELQSLRVLETHFGIDVKTDPDELAHVYDDLFLLFDHDSNGSVDMEEFKAEMKQMMLAMADGLGFLPVQMALEENSLLMKAVERESTSLFKSSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XGK9_DAUCS</name>
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        </submittedName>
    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH07710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="190" mass="21490" checksum="513F5EB6BAF9D4B5" modified="2024-05-29" version="1">MDSLSCNESTRPEIRKRVHDDSEVVELAQTDVKKRGRYDSENTESTRSESKLFKEDFLDDLDDSDICTTSPDLDLFMKSFEQEIAGAIPAKIVNLETDSGESRPDLGYLLEASDDELGIPPSEKPIDTELFRVTTESTELWRFDEQVSGYDSFDLGFNDEIYEYKSNSAEFVALDSLFDFTDSSFGLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XHF5_DAUCS</name>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
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        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
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            <end position="72"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH08160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="473" mass="50518" checksum="F259B8E0FE19119C" modified="2024-05-29" version="1">MPSSSESPPDSSTSKSPPSSPTPSSDQSPPTPSSSQSPPGSSEPPSSESPPPSPSSSQSPPASPSSDQSPPSSSTPPSQPSPSANTPTSSSNVGFNSTGSSTVNTGVNVGGSGSASISINISFKGSFSRVFAFGGSYTDTGNAQAMGALSNFFFGANSPYGKTGFGRPSNRMSDGRLVLDFLCDALALPPPQPYKSATADFSGGVNFALAGATSLGSDIFAKYKIGLNLAWKMHLSMKMQIDWFNTYYQQKICKGQDLQTCKPDFENALFWIGEMGVNDYSRSFKTSISMQMLTDSCILHINQLLMTLLQNGARYIVVQGLPPVGCLSSGVSLCPLKNLDKMGCVTIINTAIMMHNQILQKKIEGFQRQYPDRAIIYGDSWNAYMAILASPQKYQIQEPFKACCGARDGLFNFNQHSFCGFPGTTTCPDPSKYVNWDGIHLTEAMHKHITQLLLHGGFCHPSFDTIVKKKLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJ37</accession>
    <name>A0AAF0XJ37_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0728125</name>
    </gene>
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        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJ37"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH08680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="53" mass="6363" checksum="86A259E3191CBA08" modified="2024-05-29" version="1">MVERSLSMREVRGSIPRISIFFFFQTFQGKFMFCFVLFVPRVSSIRFQMLFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJ73</accession>
    <name>A0AAF0XJ73_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="4" type="ORF">DCAR_0728646</name>
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    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJ73"/>
    <dbReference type="KEGG" id="dcr:108196323"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
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    </dbReference>
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        <property type="entry name" value="PROTEIN IWS1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47350:SF4">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Med26"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="290"/>
            <end position="373"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="388"/>
            <end position="442"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
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        </location>
    </feature>
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            <end position="115"/>
        </location>
    </feature>
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            <end position="135"/>
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        </location>
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            <end position="194"/>
        </location>
    </feature>
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            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="502" mass="57239" checksum="B48E7DADDD700971" modified="2024-05-29" version="1">MAYNNDPYRDEDGEPLMDLDDAYQSDQEPQPETLVDDVDDADWRRRERSPTPVYNEDSKSKPRKRLIKKSGGGDEAVPDFGLGDDDLDDPAAGVRDESDGEYANVGEKRSGGKDKREKKKGKMERREKSGGERREKFRVKKRSRDDKEVKEMWDTIAGGDSEDDQDGVKMADDDNFIDDTGVDPADRYGSDNEPHSPGNAPQAEEGEEDDEMKDLFKMGKKKKKSEKSAAEISLLVEKVMAELEICAEEDAKLNMLSKPAINKLKKLPLLTEVLSKKQLQQEFLDHGVLTLLKNWLEPLPDGSLPNINIRAAILKILTEFPIDLEQFDRREQLKKSGLGKVIMFLSKSDEEITSNRKIAKDLVDKWSRPIFNKSTRFEDMRNFDDERAPFRRSSGKKPINKASGMQSRDDDFDLAEFSQESKSSQSSSRQHASRPEAMPLDFMVRPQSKIDPDEIRARAKQVVHDQRRLKMNKKLQQLKAPKKKQLQATKLSVEGRGMVKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKJ5</accession>
    <name>A0AAF0XKJ5_DAUCS</name>
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            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
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        <name evidence="4" type="ORF">DCAR_0729311</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="13"/>
    </dbReference>
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        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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        </location>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="10">DUF547 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>campanulids</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056001">
        <property type="entry name" value="DUF7579"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025757">
        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055437">
        <property type="entry name" value="TMEM131L_Ig_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022113">
        <property type="entry name" value="TMEM131L_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248:SF12">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1 LEUCINE-ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24474">
        <property type="entry name" value="DUF7579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24501">
        <property type="entry name" value="Ig_TMEM131L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14389">
        <property type="entry name" value="Lzipper-MIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12371">
        <property type="entry name" value="TMEM131_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transmembrane protein 131-like N-terminal" evidence="4">
        <location>
            <begin position="223"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7579" evidence="6">
        <location>
            <begin position="479"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L fifth Ig-like" evidence="7">
        <location>
            <begin position="825"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="Ternary complex factor MIP1 leucine-zipper" evidence="5">
        <location>
            <begin position="1383"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="domain" description="DUF547" evidence="3">
        <location>
            <begin position="1734"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1018"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1063"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1469"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1612"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1079"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1100"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1153"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1207"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1238"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1469"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1493"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1531"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1614"/>
            <end position="1631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH10092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1942" mass="214723" checksum="6A9BC98830221D15" modified="2024-05-29" version="1">MFLRIHVQKMLYKPPPSAEVVVFSHRDKAVCGVFKLLFTLFCIVNAGPCATSKEKNSVKCDACGPYSENFKVHYDDDFAADGDNQILSGNSAAQPSLENVCSNSNLFCFPSTLPGFLSEDKIADSADLNDSELQLDDITSTHGKSNATWASNVDSYKLLNGRVVSCSLNSLVGDHDNLCHRSSLCDQDDITSCRGTLLDRRAAGNENSAKIKSDISDGGSLQVEISPPLLDWGQNYLYKPSLAFVTVTNTHSNGILNVYEPYSTSSQFYPCNFSEMTLGPGEAASFCFVFLPTNLGISSAQLILQTSFGGFLIQASGFANESPYGIEPLLDLDRSSGRRLKKNLSFFNPFKETLYVEEVIAWISFSSGSTSHLAKAICSINSLQNHAGISDPSVQKWVGKKLSQVDMPEIVMRPHRKWEVAPQSTEIIIDIDFQHSKGTIFGALCMQVLRSSVEKADILMVPLEAEDDITSTYGDHTNPVSVSLEALLPCDESGIVLAALSLRYNVPHLLKVVKISAVGEDAQSLLFKYVEGLIIFPGTVTQVAAVTYAPVVDQSNKLESEVTNLSMNCKVLVTTNDSSNSQIEVPCGDIISICSGHGSDHLHEDVDTDLDIQPRSQLKALEMAQPDELVLLNWRSHGRSRDMSVLEEHEVLFPMVEIGSHQSQYITVKNPSHLPVVMQLILNSGEIIDDCRDSNGILQPSLVHSKDIAPARYGFSIANNAVTEAFVHPNGRATFGPILFQPSSRCWWKSSALVRNNLSGVEWLSLQGFGGSLSLVLFEGSYPVHRIEFDFNIPSFFNLSSSGMLNANNSSACSQPYLKEFYAKNTGDLILEVSKIEISGARCELDGFLVHACKGFALQPNESKKLVISYQSGFSAAIIQRDLQLALSTGILVIPMRASLPTSMLHVCKRSLLWTRVKKYWLLVVVASSLMLLIVPRIGSLVLSSYSQNHLFSGRKCPIPTLSHRGDCSFWNCNHRDSSKFTLPAKLSGFLRLLGREALVTESGARCLDGQYIEPEQEKTVTSGNLASGNSSQKRCVVNPQKESKLGVSLLSKSVALENSAMQDVSESEKLTVKVGKDKGKRRRKKKGSTSGLTGQPEVSSSQSGNSTPSSPLSPLTPFNSRKSCSKSPDVDPCLEVKSPFANPRHKKIPGPETSSKSNMSDRTGSVKYGNNSSSLNSTVKSPTPSPRPRRVATPVLLPSATFPSAGKSSPSMFTATPLPSSTSPIAPHARAPGSKLGDQRVVRTQDQKKTEDTFQYDIWGDHLFGVHRMLKSGEVYTKSRFAAKSEPRFPSTSESGFASKSDSNSFFVRVQRQNPPILVFAVFYRSWIHYCCSWMWSRGFMMIISMHDAPNPPFNLPCHIEDKRVEMQGTHRNVVASRRASSRERKLALLQDVDNLKRKLRHEENVHRALERAFSRPLGALPRLPPYLPQHTLELLAEVAVLEEEVVRLEEQVVDFRQGLYQEALYTSSRKSTEKPTTVASMTSSRGKHSRSFSQSEVNVETSPPTKPSPSTSRTTTRRRLLSSDPVCGQDEHSSDRHTSDKQCLQKLDTSLEDGLGKENQLCTNSTKVKQYLGNESAIARTPAKRPPNKPKSSEKTVDFQKQECIAVEQEIAQESSSGSSERNLETESSTANKISEDVVKCLSSIFLRLSKSKGKTMESELLSSVIALAFEGDNKFRDPYGIFSESRRDIGPYKDVSVIEACSIDLKRKTNAAFLINRLKILLGKLASVNLEGLNHQQKLAFWINTYNSCMMNAFLEHGIPRSPEMVVALMQKATLTVGGHLLNAITIEHFILRLPYHLKYNSAKSERNDEMKARSIFGFDWSEPLVTFALSCGSWSSPAVRVYTASQVDKELEIAKRDYLQAAVGISTKNKIIIPKVLDWYLLDFAKDLESMLDWVCLQLPDDVRNETVKCLERKGREPLSQLVQVTPYDFSFRYLIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XR81</accession>
    <name>A0AAF0XR81_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0831249</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XR81"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="7">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26390" checksum="5C7B5934FF4D12CA" modified="2024-05-29" version="1">MNKQSQPDITAPGVTILAAWPTETPPTLFDKRTVKWNFQAGTSMSCPHVSGVISLIKSLHPDWSPAALKSAILTTDTTHDTILGGGHMKVSDPFDIGAGHIRRIVSLSKISSATCPKGSKKAYNINFPSIMVSNLQSQITIKRTVRNVSIKKTAIYFASVVKPHGVEVSVWPSVLIFSYFKDELSYYVTLTPTKKSQERFNFGEIILFDGFHFVRSPLIVRVNTTCTSSTISADTGNDVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWD3</accession>
    <name>A0AAF0XWD3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0934757</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWD3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="15"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH15220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="36692" checksum="329FCB4E640DD2D9" modified="2024-05-29" version="1">MPIRYSYSDIKKITDNFKNKLGEGGFGTVYKGKLRSGIFVAVKLLGNSKATGKEFINEVATSGRIHHVNVVELIGFCFEGPKRALIYEFMPNGSLEKYIFRKEEVQEEIVSLSWEKMYEISCKVAGGIDYLHRGCDMQILHFDIKPHNILLDKNFNPVISDFGLAKLHATNDSIVTLTAARGTLGYMAPEMFYKNIGGISHKADVYSFGMLLMEMAGRRKKLNPFVDHVSQIHFPSWVHDQFSEGKELEMEDVTTEERKLVRKMIIVALWCIQMKPSERPSMNKVIEMLERDVEDLIMPPKPFLYPQEDPAETNNSTHSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWP9</accession>
    <name>A0AAF0XWP9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWP9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
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        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF182">
        <property type="entry name" value="OS09G0381850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="hAT-like transposase RNase-H fold" evidence="2">
        <location>
            <begin position="116"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="271"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH15671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="44729" checksum="1831F65378AABE03" modified="2024-05-29" version="1">MFHARCCAHILNLCVQDGLDPTEEIVDKVRDVVKYIAASEGCRIKFAEISKSLGLKCKKLILDVSTRWNSTYNMLACAIELKNGFKEYVPSEEDWEKVAGVCSFLKVFSDVTKVVSGTGYSTSNLFLSEIRRVKKIIDKKAIDPNLHIREMARKMELKFEKYWGETNLVMAIGAVMDPRFKMKLPAFCFPNLYPIASDSETNLSYLGNALTDLYLEYCREEKDASKEKNESEISSSDANFMAEHSEIPQGINDYESFIRESGGILEPTKSELEDYLSEKIVAPTSKFDVLAWWKGNSSKFSILSKMAADILSIPISTVASESTFSAGSRVIEPHRSCLKPETVEVLLCGADWVRELYGLKKAKQVNGYPCKNRLKIRNKSEIDLKTEVILRYIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XXW1</accession>
    <name>A0AAF0XXW1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0934432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XXW1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH14904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="17093" checksum="080280338B4ABF9B" modified="2024-05-29" version="1">MTIAEHHYLLWFNIKKPSFEVIDSSALQPDFDAKYQQIPQNIRDFLVRYMVLKNHSKAKDIASLVPVRLEMKWRTEHNHIDCGLFVMRQMEHYQGVSKNWDCGLAVEGKVQDQQLDVLRTRYARQILLHECNKQKHHVEFQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XZJ1</accession>
    <name>A0AAF0XZJ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0935309</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF58">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 5, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="808"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="183"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH15764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1312" mass="151732" checksum="389D258C52102839" modified="2024-05-29" version="1">MYSTLTTTQNPFSKIPSFPTLNRRRIKFRTHFYINSSQKPKTQIRNAVKFVPFSESLIPTRRKNENDPVLSSADNVLDEITKRVLLTVFCFVVGLCSFGGLSRPRAVLAAPVATEVVKKSKKSKGKEKELRKVKESEKVLKPVKGDYYSDYTKRLLEIVSGLLRSIKEVELGKGDVRDVKTWLKKVKLKKQELQDELLRKFDEELDIWKEEVDVLTRKSDKVLDKVMSARKERESLEDENAKEQIMKLEEEMSIGEKEYNEIMDQIDEIEDNLSMKEALLFHVAVREISYIERECQLLVENFNRNLRLKNIDSVSKSSLTKLSRLDIQNELKSTQRQLWEQMILPSVMENDDNELPFDQDSTDFVERIKKALEDSREMQRNLNSGIRKNMKRFGDEKRFIVNSPVADIVKGYPEIESKWMFGNKEVVSPRAASNHLHHSWKKWREDVKADLKKDLLEDEEFGKKYVAQRQERILLDRDRVVSKTWYNEEKNRREMDPIAVPYAVSRKLVESARIRHDWGAMYVTLKGDDKEYYVDTKEYDMLFEGLGGFDGLYMKMLASDVPTSIQLMWIPFSELSIGQHLLLMMRFAYQSWMGVWNSGNVTVVRQKIFERFKNLNEDILLVIVFPIVEFVIPSSRFKYVILKQISFLTLFLFSCWAYTYQSAGLAWYMDWLTVADMNFRSRNSLDFVWYLGFTVRTVIYGYVLLHVFRFMKRKIPRLLGFGPLRRDPNMRKLRRLKAYFRYRKRSMKRKRKAGVDPISTAFDQMKRVKNPPIELKNFSSIDSMREEINEVVAFLKNPRAFQDMGARAPRGVLIVGERGTGKTSLALAIAAEAKVPLVEVKAQQLEAGLWVGQSASNVRELFQTARDLAPVIIFVEDFDLFAGVRGKFLHTKKQDHEAFINQLLVELDGFEKQEGVVLMATTRNLKQIDEALQRPGRMDRIFHLQQPTPTEREKILLTAAKESMDNEIIDYVDWSKVAEKTSILRPAELKLVPVALEGSAYRSKFLDTDELMSYCSWFATFSNSVPEWVRKTKIGKGISKMLVNHLGLTLTKEDLQSVVDLMEPYGQISNGIELLSPPLDWTRETKFPHAVWASGRGLIALLLPNFDVVDNLWLEPFSWEGIGCTKITKAKNEGSMNGNVESRSYLEKKLVFCFGSYVAAQLLLPFGEENILSSSEIKQAEEIATRMVIQYGWGPDDSPTIYHHNNASTALSMGNKHEYEMAAKVEKLYYLAYDKAKVILQSNYQVLEKIVEELLEHEILTRKDLERIVSDNGGVWEKEPFYLSDVYEEEPVFRDLIENGNASGTALLGTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y0A4</accession>
    <name>A0AAF0Y0A4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tonoplast dicarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0935853</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y0A4"/>
    <dbReference type="KEGG" id="dcr:108202566"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH16302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="57528" checksum="DBA9CBDF9EC4C139" modified="2024-05-29" version="1">MNGDTPDHDQKSPLLPLHDQPISPKSLLSLQNFYILLGPLLAGLICLTVKLDAPSSRNMLAVLAWMFAWWMTEAVPMPITSMSPLFLFPVFGILTADEVAHAYMDDVIALMLGSFILALAVEHYNIHRRMALTITVLFSGEPLNPQILLLGICGTTAFISMWMHNTAVALMMMPVATGILQRFPTGPRSDVVTTKFCKAVVLGVIYSATIGGISTLTGTGVNLILVGMWKSYFPEADPITFSTYILFGFPLALFIFFAMWIILCLWFVPKGSGPTLSTYLDKSHLKRELDMLGPMAFAEKMILTIFSILIFLWMTRSITDDIPGWGALFNGRAGDGTVSVMMATLLFIIPNKKQPGEKLMDWNKCKKLPWGIVLLLGAGLALAEGVQTSGLTNVLSKALDFLEQVPYLAMAPAVCLICATLTEFITSNNATTTLVLPLLIEIAKTMHVHPLLLMLPGSIGAQLAFLLPTGTPSNVIGFTTGHIDLKDMLKTGIPLKFAGIVVLSVVMPTLGAFVFGTDSKHWSIGKSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIV0</accession>
    <name>A0AAF1AIV0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIV0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009902">
        <property type="entry name" value="DUF1442"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33593">
        <property type="entry name" value="DUF1442 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33593:SF16">
        <property type="entry name" value="OS08G0110600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07279">
        <property type="entry name" value="DUF1442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="229" mass="25638" checksum="57D608A3DE1FE842" modified="2024-05-29" version="1">MEWSPPDAMTAFFRTSDLCKIHRQAGRQGSTRQVFEPECMEFVSALAAGRQAKLIVEITTQGITPLTLALAVAATQTGGHLICIVPHKTTKNIISNQLKQHFELDKVARIVIGDPCQVIKHYKSIDFAVIDGKYEDHLRLFKIIIDMKQKGSVVVVNSKVCRKSFGEVVKGKRGSVESVVSFPFGQGMELTRMGSSNSCRSGKSRRFYTVEELIRQQKIYLLCQFVPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJK2</accession>
    <name>A0AAF1AJK2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0104159</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJK2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF28">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG84973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21188" checksum="E01BAE17D99EF724" modified="2024-05-29" version="1">MDEAVWENFLFCTGKDTIVYIGDGELVREMSLCKSLNLGKPSYMHKERGPLLGRGLLTTSKEVWVHQRKTIAPTLYVDKNMFSIVLESGNTLVKSWEHLVDTEDGIADMRVDDYVKTFTSSIISNVMFGKYEAAEKLLFSRCRDLMEVSGSPTVLDGYPFNRFYPTKIHRQQWKLEKEIYWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQX9</accession>
    <name>A0AAF1AQX9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dethiobiotin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0311188</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQX9"/>
    <dbReference type="KEGG" id="dcr:108215280"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000088">
        <property type="entry name" value="Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000090">
        <property type="entry name" value="Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF3">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG91933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="863" mass="95675" checksum="B5D32726E9A4CB0A" modified="2024-05-29" version="1">MPLLFSTLSTATRRRVYLKSLFSSSRFISTTSFALEYPLSHPIYTVWGSNTAVGKTLVSAGIAASFLTPTSTSPFKFIYLKPVQTGFPSDSDSRFVYRKFGEIFGHRKCNRTVFASDETVRVSAPAGELVLGSRGSVETQNFTKCESGFWELGSCDGRKLEGEGGEEVRVSELICKTMYAWRDPVSPHLAAERENAVVADAELLEMLKKCLCIGSDDGMREDEVGVFTLIETAGGVASPGPSGSLQCDLYRPFRLPALLVGDGRLGGISSTISAYESLKLRGYDVVAVVLEDQGLVNDVPLLSYLRNRVPVLVLPPIPADMSDNLMAWFDESYSVFGSLKEVMLSSYSERMQRLHDMPKKAVEIFWWPFTQHKLVPEENVTVIDSRCGENFSVHKGKYQDVMIQQFDACASWWTQGPDATLQIELARDMGYTAARFGHVMFPTNVYEPVLKCAELLLEGVGKGWASRSYFSDNGSTAIEIALKMAFRKFLFDHEEHVASWGSDMKGTHVELKVLALKGSYHGDTLGAMEAQSPSPYTGFLQQPWYSGRGFFLDPPLVHICDGTWRLSLPGKLRSYEEKVADMFFGSRDEIFSKSRDDSSLARLYASHISEELLVFSAENLSAHIGALILEPVIQGAGGMQMIDPLFQRTLVNECQYRKIPVIFDEVFTGFWRLGAESATELLLCKPDIACFAKLMTGGIIPLAATLATDAVFDAFVGDSKLKALLHGHSYSAHALGCAAASKSINWFKDHRTNHNLKTEGSMLRELWDVELVHQISLHPVVHRVVSLGTVFALELKAEGCNAGYASKYANNLLEKLYEDGVYMRPLGNVIYLMCGPCTSPATCERLLEILYKRLQEFGKEQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQY9</accession>
    <name>A0AAF1AQY9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0208276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQY9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF344">
        <property type="entry name" value="OS07G0636900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="411"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="609" mass="68652" checksum="7228830289BFC1C0" modified="2024-05-29" version="1">MPALTNQPPFKTLLSYFHLNKTLPLIQPLHAKILTNGLQFSSLYASKLANVYIELGFLRSAYKVFDQITHKNLYSWNTMLSGYSKNNQSFDVLRIYKMMRSDIGRSDSFNLVFVIKACAELSRFRDGEAVHGLVVKLGFESDQFVVPRLINMYIDVECLDEAEKVFDVCCEKDLVVWGCMVKGCLRLCKYFRVFDFVRRMIMSGSEMDTSLLEMLVQACGNVLAGKEAKSCHGYLIKNKFLNCGISLQTSIIDSYMKCGFCDLGVKLFEEIPEKDVVSWTAVVAGFTKLGRGLEALGLFRQMLREAVAPNPVTFSSVILACSHMGSLELGKSVHCYMLRHAIELDTVNYTSFVDMYAKCGCIMMASRVFNLMPHKTVYSWTAMINGFGAHGMYSEAINLFDEMISQNQQPNSVTFVSILSACSHSGKVEEGWKYFNIMCREHGIVAKEEHFACMVDLLGRAGRICEALSLIDKMPMVPGASTWGALLSACKIHKLVELAEYVAKKLLPVETDKSSVYVLLFNIYADAGMWDKVKKLRLRFGEKGLHKSAGFSSIEVNEKLYVFTSKTKLASLFTELESVWISVTEQMRIFGHVYDMSFIINDAEDIVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AS59</accession>
    <name>A0AAF1AS59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0312534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AS59"/>
    <dbReference type="KEGG" id="dcr:108214587"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21039">
        <property type="entry name" value="NURR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="98"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="201"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="494"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="570"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="611" mass="67360" checksum="C965B0CE9F0ABFFB" modified="2024-05-29" version="1">MSNSSENHPAGNKNVVKGSEVFVGGLPRNLTEDRIREVFSACGEIQEIRLIKDHQGTLKGYCFVRFTTKAAADKAVREKSGLVLDGKKIGVLPSSEQETLFMGNLYKGWTADEFSNLVRQVFPDIVSVDLATRVSSGQSPPGQKKHNRGFAFVKFSSHAAAARALRAGQSPEFSLGGKVHPAVDWAEEDPEVDPEKLAKVTIAFIRNLPGGADENFLKKLFMPFGQVEKVIISNKTRAPVGFVHFAERSELDDAIREMNGKTVQGPNGGSPFKLLIQVARPMDKSKKRDREEPQSKQPNKVLVHSKLVRTDPAITVGLEDRALQEAVAVDPYEAAVYPLPAAIKERLLRILRLGIATRFDIPIECLRSLKELPEPTAISVLDQFMISGADQDNKGAYLTALISKHQVTKLGLVGLYGFPSSASRVTDMSTKEPELSSYSRRAQYPAYDSTSSHLEYTATRSDGHPSRYSYMYSEYPPSRALDYPLPSQAADYPLPSRASDYPFPSRTSDYTLPSRASDYTLRSRASDNPLPSRASFGKLEAASPASQSTPGFLTPCGKIGLDSHVTAAANHQPVRPQMRFDPYTGEPYKFDPFTGEPIRPESPQRNFRSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AS78</accession>
    <name>A0AAF1AS78_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0312594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AS78"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF162">
        <property type="entry name" value="MINI ZINC FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
        <property type="entry name" value="ZF-HD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="7">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG93313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="90" mass="9679" checksum="B7D9006538AD525C" modified="2024-05-29" version="1">MKKVVRKDDSNNNGSHTNSSSTVTRVTYGECQKNHAANIGGYAVDGCREFLASGEEGTEAAFTCACCGCHRSFHRREVQTEVVSDSSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW35</accession>
    <name>A0AAF1AW35_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0414626</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW35"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33067:SF32">
        <property type="entry name" value="ASPARTIC PEPTIDASE DDI1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33067">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG95312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="829" mass="93767" checksum="48CEC2A1A2901BAE" modified="2024-05-29" version="1">MCQGKFMEKDEDQGWEFFEDLAVKTMMWESTREPKKSIQSSSARGLHSIGNNVATDAKLATLTRRLEALETSKPPPQMSMLPNYNFQNPGTQPSHEFEQVNAMFQNSRNDAFAPTYNPGWRNHPNFSWTQGQIFQNQGPNSFPTPQPNFQQRPNPNSYPNQGPSSCRNPIQTPLNPPGFNDSDKRLNSLEKSLETLVKSQTNLTQSQQTFMTTLTQDRQILHSNVQAVSKLEVQLSQLASALCEREKNKFPSQPEVSPKFPLNQRPPENVNAIISLRSGNLVDNKVGENANENEESIPKTNPSLPSTNHDKPECSRIRESMSEPSPGPISQKPNEEVYKPRVPYPQRLIPPKQSAQMEQILEVFKQVKINIPLLDAIQQVPSYAKCLKDLCTHKKTTHVPKKAFLTSQVSSILSNQIPVKYKDPGCPTISCVIGDKFVDKALLDLGASVNLLPFSVYQALGLGDLKTTNMTLQLADRSIKMPKGIIEDVLIKIGDFIFPVDFVVLETQPIVSVCPRPDFASHVGETPTFDSLFPHSHRALGEDDIISYIVPSDLSYDQNCIVSNLLNNKEALNFIFCSYILVLYVFFVACLRFVLSSVYSVNSEQLFRVQCLHNNIRRKMINFFIYFMNTNIHFSNVQLIKIFDSLEYFLSNLFYELVDIKTNHNLSYKYKPTVNRFQKGGYSSSTSKGGYKTGMVDRSKFKCFNCGEPGHFATECKQPKNFKSVNNVRVESIDVPTTMTDMPAAPPSISPRGSVVLPDRNQFFKFAGPNQVWVPKKRDLVRGLPSQEFSTDDLCEALFSTKDSSAEWSRQKKQSCDYCLLYPESNFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYR4</accession>
    <name>A0AAF1AYR4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">DCAR_0416660</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYR4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000661">
        <property type="entry name" value="Serine-threonine protein kinase plant-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF73">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041902381" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="836"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG97320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1129" mass="125537" checksum="E53CF23E73218C27" modified="2024-05-29" version="1">MAKTCLFFALVLAALISTSCILCSSTDQSALLAFKSYITDDPIQILAKNWTRGSDMCSWIGVSCDASQEHVTALNLSMMSFKGTIAPHIGNLSFLTSLDLKSNQISGSLPIVLFNMPALRVINLSDNALSDNLPVNMCSNHSRLKALYLSSNQLHGEIPASLYSCRELQYLSLENNTFDGRLAKEIGNLTNLKHLYLGTNVIHGEIPLQIGNLRSLEHLRMGSNFLTGPVPSTLFNISSLETINLSRNKLSGVLSSDIQWNLPLLRDLNLQTNLLTGQIPSGLWGCKLLQMLSLAENSFTGSISKQIGNLTLLKGLNLDTNNFTGTYKIQWLSVHILAGVLPAEIGYLNLELLFVPSNKLSGEIPYEVFNSSTLRIITLQFNQFYGHLPRGIGLWLPNLEELYLGNNRLSGSIPVSLSNASKLTILEMSTNQFSGYIPNTLGSLRSLRRLHLGENNLTRESSSTELRFFYSLTNCRYLQVLELSMNQFHGSLPAVVGNLSTSLQIFSAFNSRIKGEIPIGIGNLSSLNMLTLDSNELTGIIPSTIGRLKYMERIYLEHNKLNGSVPYEICLLEKLGDLYLSDNQLSGLIPACLGELPSLTRLYLDSNKLTSNVPSTLWKCVDLIGLNLSTNYLSGYLPSEVGNLRAITEMDFSWNQFSGNIPATIGGAQSLNYLVLSHNKLEGPIPPSLGNLKGLEILDMSNNNLSGKIPKSLESLRYLRYFNISFNELEGEIPTGGPFQNFTGQSYLQNDGICGEPRLKVRMCPASATQKESGSRNIAFLKYTLPLIVAATLLVAVAFLLKRRRNQKIILTQEDSLPHSLRRFTYHELLQATNGFGESNLLGVGSVGSVYKGELLDGTVVAVKVFNMQFEGASKSFDAECEVLRNVRHRNLTRLISSCTHLDFKALILEWMPNGSLEIWLHSEKYCLNFLQRLNLMIDVASALDYLHCRCSTPIIHCDLKPSNVLIDNYMIAHVCDFGIAKLLGEQEFLAQTKTLGTIGYMAPEYGMEGIVTARGDVYSYGILLLETFTRKRPTEEMFSGEMSLKDWVKESLSSSEVLDTKLRERNDKYLPIKNLCALSIFNLAMDCLRNSPVQRIDMEDVLNKLYKIKTLFLEQCHLIDSEVNDYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1J3</accession>
    <name>A0AAF1B1J3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0522518</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1J3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27004:SF447">
        <property type="entry name" value="RECEPTOR LIKE PROTEIN 30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27004">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 12 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH03124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37844" checksum="1E432ADCA375B9CC" modified="2024-05-29" version="1">MMDMSQNQLTGKVPKSLSNCKMLQVLDLSNNQMKQTFPTWLGTLPRLQVLLLHFNMFHGEIGSPRSPSEFPLLSIINLSHNAFTGALPVNYIQFWNAMKVVRTGIEPYIETYVYISFSHSKYPYRDLYHYYNPMTLTNKGAEREYKEVLNCFTAIDLSSNKFTGQVPESLGSLKALQLLDLSNNDLTGPIPPSLGNLTQLESLDLSQNKLWGVIPEQLAAQLNFLSFFNVSHNHLSGPIPQGPQFRTFDYNSYIGNSGLCGFPVSKNCGTPQSPPDDNEDGSEEEKFPSGFDWLFILVGLGSGLVVGFVMGDISMDRHPWLIRGIVQKFGLTEETENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2L8</accession>
    <name>A0AAF1B2L8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0521677</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2L8"/>
    <dbReference type="KEGG" id="dcr:108223139"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058954">
        <property type="entry name" value="AAA_lid_SMAX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058680">
        <property type="entry name" value="NBD_SMAX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051650">
        <property type="entry name" value="SL_signaling_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43572">
        <property type="entry name" value="CHAPERONE PROTEIN CLPD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43572:SF38">
        <property type="entry name" value="PROTEIN SMAX1-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26587">
        <property type="entry name" value="AAA_lid_SMAX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23569">
        <property type="entry name" value="NBD_SMAX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Clp R" evidence="6">
        <location>
            <begin position="8"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH02288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1090" mass="120828" checksum="D74141F77FC6AA71" modified="2024-05-29" version="1">MPTPVSTARECLTEESARVLDDAVSVARRRSHSQTTSLHIISALLSMPNSTLKEACQRNRGGAYSPRLQLRALELSVGVSLDRLPSAKTQDESESLPPISNSLMAAIKRSQANQRRHPETFHLYQQTLNQTHQNGVVLSVKVELKHFVLSILDDPIVSRVLGEAGFRSSDLKISILQPPAQIRYPPPLFLCNLVNDESKRCRFSFPFAIESVDENSKRISEVLVKKNKKNPILIGFCAKDALKGFRESVKNGKVGVLDKKIEGLSSICIEDEVSEFVLKNGSEEMMGGKFDEVGDVLEKCRGCGVVVDFGELGVFVKGVSIEGLSYVVSRLSNLVRVYGEKIWLMGFAESYEIYMKFVERFPTIEKDWDLHMLPITASTPLNGGSYSKSSLLGSFVPFGGFFPAPPEINNLLESRSQSAPRCDMCNKKYEQEVSSVLKGGSTTSVAAQDQLNLPSWLQMDDIDKCKSANPSEVRDGGVQNARLAGLQRKWNDICQRLHHPRSLQQEMSKVGSLLPAVGSYHSDAKRKDDNGKDCLLNECIGADPCSCISSSFPKISSRPKQYMEIPVTSVAEIPVLQNQGTVEVSSIREPEMNHKEPSYPICSPLLQPGLTSSSSVTSSVTTDLGLGTLYASCEQEHRSSKSQDFKELPKVSWYIPAKISGDCTLTSANDSMQTSFRPSLGGQSDDKDFKYLWKVLSDTVGWQEEAISTISQTISSCRNGYGRLRGQTYKRDIWLSFLGPDKVGKRRIAGALAQISRGDLFSVDLDPVNCFSLQNSIFDYPDSSSNNLSIRGKTIVGYIAEKLSRKPYSIVLLENIDKADFITQRSLTQAIKTGRFPDSDGREINISNMIFVTTLSRDINVMEKGPPKFTENKVLGAKGLQMKIFVEGNAVDIPKTRSENVLLEPMKGTSNQVSVNKRKITDEIGNSPEVVNRFQKVSRTCLDLNLPVEDTEDDDYGACDSHSSSESSDVWLEDFLEQVDQKVVFKPFDFETLAQKILKNIEKSFRETIGFDILLEIDSEVMLQMLAAAWLSNERAVENWVEQVICKSFVEVKQKHHIASGSVLKLVALEGLQMGDKAPNLNLPANISVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B3X2</accession>
    <name>A0AAF1B3X2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0522474</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B3X2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042138623" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="319"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="635" mass="71804" checksum="C9165EB89C2EEB0C" modified="2024-05-29" version="1">MNRVILNVFKFLVSVSCIMSNHGSAVLQDDECSVTRCPGGDEIRFPFHLIYPDKERQLQSDHCVFPVGFELSCGYYYPVVKFEYQVSTPLPGLHLSFSVAAYIDSIDYKSRQLHFISKSTNVSQHYGYYFVNSPFKPFTSSKVKAQFTYTDLRTDFTFYKCSSTKSEMDIDGVEVPSLSGHGFKVYAIYSHFRMTEIVLTSCTKLFNISHVPFNGGGLSWSEPDCGDCESKNQYCKFKPNSTTATECFPIPKVISELYMCLGKVAGIFVLSLLLAAICYAIYSYKQKIIFQQKIEIFLEDYKSLKPTRYSYADIKKITNHFKVRLGEGGYGSVFKGHLSNDVAVAVKVLNDNVDAKGSGQDFINEVSTIGLIHHVNVVRLVGYCADGCRRALVYEFQPNNSLEKYVYSRENQRNGFLGWEKMQKISLGIAKGIEYLHQGCAQRILHFDIKPQNILLDKKFNPKVSDFGLAKLCNKDQSRISMTMARGTVGYIAPEVFSRNFGEVSSKSDVYSFGMLLLEIVGARNHTSTGTENESEVYFPEWIFHQLEQRETITSPIEEDLDSKIRRKLTIVGLWCVNWHPADRPSMKHVIQMLQGEDCPVMPPNPFSSTGSRNELAGGPGRLFTSELEVISELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B489</accession>
    <name>A0AAF1B489_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">RIN4 pathogenic type III effector avirulence factor Avr cleavage site domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0522005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TypeIII_avirulence_cleave"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="22"/>
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            <end position="73"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="13"/>
        </location>
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            <begin position="40"/>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="89" mass="9799" checksum="EADE55007F84208E" modified="2024-05-29" version="1">MSLLKDSDLDNDKGLPLPKFGEWDVNDPASAEGFTVIFNKARDEKRTGGKADSPPKDGSNPSMFKRKTTFGKPQSRKWFCCVQSASAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B5R7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DCAR_0626634</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH07205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108226374"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
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    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042073812" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH07205.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="42723" checksum="3E2D046B82B1E9B1" modified="2024-05-29" version="1">MCLSFLSSKVLVLVFMVSCINQVNSQKFCNVDDFGAVGDGKTDNTNAFRNAWKQACQSGGAVSMVNGTYLLNTIQFTGPCNGQVSFMINAVIQAHQGRSNTNYWINFDNVDGLTIRGNGTLDGQGASAWPFNPCSNAVSCLPLTPSLMLTRIKQSLVHNITLLNSKGFHMKIENCENITIKSITITSPADSPNTDGIHTGNINYVNILNSSISTGDDCISMGAGTRNINITGVICGPGHGISVGSIGKYPSEQNVEGVNVRNCTMRSTQNGVRIKTWNSTFQIYVSDVTFQDIVMDNANNPIIIDQQYCGGGHECIGSSHVQVKDVKFIRVEGTSRSKIAVNLNCSSSKPCRGIELNNINLSLADGGNATSSCSNASVSYIGTQNPAPCQENYLYYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B6N8</accession>
    <name>A0AAF1B6N8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0727647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B6N8"/>
    <dbReference type="KEGG" id="dcr:108196279"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH08210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="307" mass="34409" checksum="B2E8EF88A752D65B" modified="2024-05-29" version="1">MYEVDKETSEKIDYSSTICRCTSLKYDEFDKIELDEKDPEGNVTEEESEESEKLVDSLSLAGQQLKVLHNVDEIVAWKHRLAKLDLSSNLLSTLPGSISVLTNLKVLDVSRNNLSGLPESISHCRSLEEFDASFNELKFLPNNFGHGLVHLKRLSINLNKIACLPASIREMVSLTYMDVHFNELHGLPYSIGELKNLEVLNLSSNFNHLTTIPDTIGELSNLRELDISNNQIRVLPSTISQLVNITKLNLDQNPLVTPPAHISAKGIEAVMNFMKSRYVNNSAAAKRVCAAKNDERGPSEWHQALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7L4</accession>
    <name>A0AAF1B7L4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0626245</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant may improve abiotic stress tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="8"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7L4"/>
    <dbReference type="KEGG" id="dcr:108225322"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000073">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF27">
        <property type="entry name" value="TREHALOSE-PHOSPHATE PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH06817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="384" mass="42837" checksum="638A2C758D58A154" modified="2024-05-29" version="1">MELETNHNASVLNNSSPKNNSRLGPHSGLPKYPTSGAVSSSSLHLTVPRKKTGLLDDVRLSGWLDAMKSSSPTQLEPSRDSATELTDKEIAYRNWMLKYPSALASFDKITTYAKDKKVVLFLDYDGTLSPIVDNPDQAFMSNNMRMAVKNVAKYVPTAIISGRSRDKVLEFVGLTELYYAGSHGMDIMGPVSANTDGHTNCVRSTEKQGKEDNLYQPASEFLPMIDEIRKSLVEITKDIEGAKVEDNRFCVSVHYRNVEEKSWTTIAQSVHDIMKNYPRLRLSHGRKVLEIRPVLDWDKGKACEFLLESLGYSNCDTVLPIYIGDDRTDEDAFKVLRAGNQGFGIFVSSAPKASNAFYSLRDTSEVLEFLKSLGKWKEASALQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BA05</accession>
    <name>A0AAF1BA05_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0831410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BA05"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH11914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="17777" checksum="D4A774E1FD8B6324" modified="2024-05-29" version="1">MPKCAFFFWLAIKNRLLTKDKMRLFEMQVDPRCVLCNGGLESSHHLFSNCPFFAALRAESPVQFNADWDLCRTGAFFIGNTSRIKKRIGGLFIAVAIYHTWKERNFRIHNVGKHNSPSRVLLRVKEMVRERLFSCNSFKRMAANHPGIIPLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCW6</accession>
    <name>A0AAF1BCW6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">DUF936 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0831121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCW6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049172">
        <property type="entry name" value="DUF6857_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048297">
        <property type="entry name" value="DUF936_dom_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010341">
        <property type="entry name" value="DUF936_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31928">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31928:SF4">
        <property type="entry name" value="OS08G0541500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21647">
        <property type="entry name" value="DUF6857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06075">
        <property type="entry name" value="DUF936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042258994" description="DUF936 domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="DUF936" evidence="3">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6857" evidence="4">
        <location>
            <begin position="406"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH11631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="737" mass="78831" checksum="C7C75745C29D5B4F" modified="2024-05-29" version="1">MASLVPGVLLMLLQHMNTDVKVGGEHRSSLLQVVGIVPALAGGELFSNKGFYLKVSDSSHATYVSLPDEHVDLILSDKIQLGQFIHVDRLEAASPVPILYGVRPLPGRHPCVGTPEDLVATHSLGFLSNDANLLSGSKNLEKSKPESKVLGGTSGCGSVGMRRDKKFASLKLGGGLKDDLGNGESPSLIRSKSQLLKVGVGSVEKKEPALKKSLSSRSIPSSPMSCYSLPNSFEKFSNGIKQQGKIGGFGKTTKLASGEKASSVRGTSPAKTKTVAGSSVSNFIQGIGSGPKALRKSWEGNMDVKARDSPRLRTTKSNLKPESPSFLVPRKSTSERLPPKEETKVKVSSKSVKEENKAGLAANRATTNGALVDPNRSHKQMLSVGKKTPGNVANHGLPGNFNKVSLSKKRLTEGSASWASLPSSLAKLGKDVLKHRDAAQMAAIVALEEASAAESLLGCLSTYSDLSSSAREDNPQTAVEQFLTFHSSLKSCHQIVSSLYKATTTGSSSDGEKVPSDEALKVTSERRKQAASWVDAALMTSLSSFSLYTKQSLSSTAPISTSSPNQKTVLANEPILVLENSSKSTSTKTQAKPRQTVSSKITGQKPKVPPPLPPQVEWVRGDGLYEGVDLAEMLQMESQDWFLEFLERFLDAKVDVSTLSDNGQIANMLTQLKSVNDWLDGIATTKDEEENTHISPDTIERIRKKIYEYLLTHVESAAAALGSGSQSSPPTETNIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BGR9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0935561</name>
    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 39-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH16012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="90" mass="10172" checksum="139F27D43D47E9A7" modified="2024-05-29" version="1">MIVSITPNQQVAAIFAWIWYYYLYPVAWTVYGIIVSPYGDVTDTITVPGMSTKPTIKWYINDHYGYESDFMAPVAVVLIGFTVVLVCLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161WUA0</accession>
    <name>A0A161WUA0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161WUA0"/>
    <dbReference type="Gramene" id="KZM92167">
        <property type="protein sequence ID" value="KZM92167"/>
        <property type="gene ID" value="DCAR_020468"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225573"/>
    <dbReference type="OrthoDB" id="1916775at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051783">
        <property type="term" value="P:regulation of nuclear division"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034590">
        <property type="entry name" value="POLYCHOME/GIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35119">
        <property type="entry name" value="PROTEIN POLYCHOME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35119:SF1">
        <property type="entry name" value="PROTEIN POLYCHOME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="227" mass="25669" checksum="677B05B5BDC95FEC" modified="2016-07-06" version="1">MPEARDRLLRPGNDAEQLFNRLRRSSGSSEIRSDDSPVVSTGTAGPSAVAAARATASARRRRNLNRYSGGENRQVGRRVSRRVLPYWYPRRPLQDITAIVRAFERKRARLREVDQRFESPLPYGQFVHDLSESSSGAPLEHLSLITPKPTPLTRRNPPFLGKVSDILRDVTNQEAGESEFLTPEKKLLDSIDKVEKAVEEEISRLKETPAAKRAVRKAKVRTLMSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y4G0</accession>
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    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0205519</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161Y4G0"/>
    <dbReference type="Gramene" id="KZN04167">
        <property type="protein sequence ID" value="KZN04167"/>
        <property type="gene ID" value="DCAR_005004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006712">
        <property type="entry name" value="HD-ZIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF16">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04618">
        <property type="entry name" value="HD-ZIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="142"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG86317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="266" mass="29282" checksum="77AB25640EC8FA72" modified="2016-07-06" version="1">MVQQRDKWGLSLSLSFPGSSNHHHKLTNNNNQTAHVSSPLELNLVPSATSNPLPENTTQKSHSSGPNMETCRVDRRCSLTGIDLEVDQRCSLTGIDLNGLPAADIGDEADRVLSPNRTQSSLSGNKRSLIREGAGGENNQKTALAKRLGLKHRQVEVWFQNRRARTKLKQTEIDCEFLKRYFHALTEENAKLKKEVQELRAFKLSPQFSIPPTTLTICPSCEHFSKSSSAPTLSVCPSSTHSKQPQVHAMSASTIDSISTCAPNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZKD5</accession>
    <name>A0A161ZKD5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine protease</fullName>
            <ecNumber evidence="13">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_023584</name>
        <name evidence="17" type="ORF">DCAR_0727054</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of PE-conjugated ATG8 proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with ATG8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="13"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZKD5"/>
    <dbReference type="STRING" id="79200.A0A161ZKD5"/>
    <dbReference type="Gramene" id="KZM86450">
        <property type="protein sequence ID" value="KZM86450"/>
        <property type="gene ID" value="DCAR_023584"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196810"/>
    <dbReference type="OMA" id="TGFGCMI"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="15">
        <location>
            <begin position="139"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZM86450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH07621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="486" mass="53998" checksum="BAC6B057DFFA840D" modified="2016-07-06" version="1">MKSYRDRTDTCTYPDRSFIESPKDHPGSNNLEPGTSENKHTNTSSWSGLLVSAFSNFEKYSAPKLFTKRESSTKRHGWTATVKRVMVGATMRRFHERVLGLNKNGISISNSDIWLLGVCYSLSNEDSSADPIQSHGFAAFVEDFESRMLMTYRKGFAAIGESKYTSDVNWGCMLRSSQMLVAQALVFHRLGRNWRKSLEKPLDKDYIEILHYFGDSEESVFSIHNLIQAGKLLSPGCWVGPYAMCRTWETLARCKMDDTELENQSLPMAMYVVSGDGDGERGGAPVVCIEDASRHCREFSGGQVDWSPILLLVPLVLGLDKINPRYIPLLAATFTFPQSLGILGGRPGVSTYIVGVQDENAFYLDPHEVKQVVDIKRDNLEADTSSYHCNAIRQISLELIDPSLAIGFYCRGKDDFDDFCSRASGLAAQSNGAPLFTVAQSCNSMKPARHCETSCDSAEVHDDDSFSAVPTADADNGAQEDEWQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZN62</accession>
    <name>A0A161ZN62_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_025917</name>
        <name evidence="12" type="ORF">DCAR_0729773</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZN62"/>
    <dbReference type="STRING" id="79200.A0A161ZN62"/>
    <dbReference type="Gramene" id="KZM88842">
        <property type="protein sequence ID" value="KZM88842"/>
        <property type="gene ID" value="DCAR_025917"/>
    </dbReference>
    <dbReference type="OMA" id="FQVPPMH"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM88842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH10306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="503" mass="57301" checksum="E12FF85074AF0A1D" modified="2016-07-06" version="1">MEKTTMEKGDYKNSAVLFLCISVFALLVRVAVSLHPYSGAGNPPKYGDFEAQRHWMEITLGLPPQEWYRNSTINDLSYWGLDYPPLTAYQSYVHGLLLRYFHPDSVSLYSSRGHESYLGKLLMRWTVLLSDALIFFPAVLCFVTAYYSGQQGTHKNNIAWHTTIILLSPCLILIDHGHFQYNCISLGLTVGAIAAIISGKELVGSSLFCLALNHKQMSAYFAPAFFGHLFGKCLRRRNPYIEVLKLGLVVLGTFAVVWWPYLHSLNSFLEVLSRLAPFERGIYEDYVANFWCTTSIMIKWKRLFTTQSLKLLSLSATLFTCLPSVVQQIRYPSSHGFLYSLLNSAFSFYMFSFQVHEKSILLPLLPASLLALEEPSIFQWLIQFALLSMFPLLCRDKLVIPYAALYGIFILLYNAPGWKQDARERSSTSTAKSLLVAVITMISVLLHVIYLTISPPRRYPFLFEAIIMLLCFSQFIAVAVYTNKKQWMLSKQHSAPMAKEKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A2P1</accession>
    <name>A0A162A2P1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_018504</name>
        <name evidence="7" type="ORF">DCAR_0521178</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast stroma</location>
            <location evidence="1">Chloroplast nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A2P1"/>
    <dbReference type="STRING" id="79200.A0A162A2P1"/>
    <dbReference type="Gramene" id="KZM95262">
        <property type="protein sequence ID" value="KZM95262"/>
        <property type="gene ID" value="DCAR_018504"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220231"/>
    <dbReference type="OMA" id="KVYWDAK"/>
    <dbReference type="OrthoDB" id="1920727at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042644">
        <property type="term" value="C:chloroplast nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000195">
        <property type="entry name" value="thioredoxin-like fold domain-containing protein MRL7, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044701">
        <property type="entry name" value="MRL7/MRL7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34669:SF2">
        <property type="entry name" value="THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34669">
        <property type="entry name" value="THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM95262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH01793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37950" checksum="B8BDEFE69266A651" modified="2016-07-06" version="1">MFLSAAKPCLCFDSCSTLYPSISPSLKISYSRNNVRLNVSSKKSEPEQSSDARPHSKPIEKGQHRESRASQNEVNGESDGKVYPTTIPKKPRRGRRSEAAAVEDFMRDSLERTFASIREQNSEVMKDKEQIMKEKIDDAVESDDVDDGSEESVKSKKMLVEEDNPDWPLDADVGWGIRASDYFEKHAIRNVVGEDGVEIDWEGEIDDNWVKEINCLEWEAFAFHPSPLIVLVFERYNRASDNWKVLKELEKATKVYWRAKDRLPPRAVKIDINIEKDMAYALKVREGPQILFLRGNRILYREKEFRSADELVQMIAHFYYKAKKPSCMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A3S2</accession>
    <name>A0A162A3S2_DAUCS</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A3S2"/>
    <dbReference type="Gramene" id="KZN08436">
        <property type="protein sequence ID" value="KZN08436"/>
        <property type="gene ID" value="DCAR_000982"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="77" mass="8272" checksum="874501E8F621BBFF" modified="2016-07-06" version="1">MCFWFIKFFKNGVRQCLGLEAEPSPAGTSDGSKSSAHIEGEEEEALDVSFSAGTDYTMPVDTDQETPATVSSLPLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AEL2</accession>
    <name>A0A162AEL2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_008757</name>
        <name evidence="4" type="ORF">DCAR_0309892</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AEL2"/>
    <dbReference type="Gramene" id="KZN00003">
        <property type="protein sequence ID" value="KZN00003"/>
        <property type="gene ID" value="DCAR_008757"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210547"/>
    <dbReference type="OMA" id="IETCTLI"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol transfer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF22">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="81"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN00003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG90648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="33751" checksum="27C50B6667E04FEB" modified="2016-07-06" version="1">MFRRWSGSQPSQENKEPLPEVKINELRVALGPLSGRSLRYCTDACLRRFLEARNWNVEKAKKMLEETLIWRSTYKPEEIRWHEVAGEGETGKLFRADFHDRYGRTVLILKPGLQNTTSIDNQMRHLVYLIENAILNLPEGQEQMAWLIDFTGWSFSTSVPVKSAKDTISILQNYYPEILAVAFLYNPPRIFEAFWKVVKYFLDTKTFHKVKFVYPKNKDSVELMKTYFDMENLPTEFGGKTTLNYDHEEFSRLMAKDDVKSAKLWGFDGKQKPAYSGAEVVPEPETLPSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AG15</accession>
    <name>A0A162AG15_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_009125</name>
        <name evidence="8" type="ORF">DCAR_0310320</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AG15"/>
    <dbReference type="Gramene" id="KZN00371">
        <property type="protein sequence ID" value="KZN00371"/>
        <property type="gene ID" value="DCAR_009125"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211036"/>
    <dbReference type="OMA" id="MREEEDH"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044841">
        <property type="entry name" value="LUX/BOA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442:SF29">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN00371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="291" mass="31898" checksum="01B29909DDE526F9" modified="2016-07-06" version="1">MREDESNWFSKWEEQLPSPDELMPLSQTLITPHLAVAFDITNNPVINTSPGGVATLQVQHSLQPPPPLSMPLQSPNSGEFDSSELGGGGAAGDEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGLSGSGGGSHASSGDAATDHLFASSPVPAHFLHQGRSNSEHFLPFVPVSALQQQQMVAVAGHPQYRHVPHFGSPSNAQFEHPFMARQPHQQQQQVHRMGTPVQNTLQSSYIEDLESGNARKVLTLFPTGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AH80</accession>
    <name>A0A162AH80_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_009787</name>
        <name evidence="15" type="ORF">DCAR_0311054</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AH80"/>
    <dbReference type="STRING" id="79200.A0A162AH80"/>
    <dbReference type="Gramene" id="KZN01033">
        <property type="protein sequence ID" value="KZN01033"/>
        <property type="gene ID" value="DCAR_009787"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212217"/>
    <dbReference type="OMA" id="TPTINIM"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF255">
        <property type="entry name" value="CYTOCHROME P450 72A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN01033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="58929" checksum="01AE70A8F8054D3A" modified="2016-07-06" version="1">MAASFLEIAIAIAVVAATTVAWRVFNWVWLRPKKLEKHLKKQGFHGNSYRFLYGDSKENMSMLMATRSNPVPISHDVPSRTTPFLCDLVRNYGKRTFFWAGPAPRVVIMNPEMVKEVLNKNSKYQKPKEHPMFKLFIHGLPTIDGSKWTTHRRLLNPAFHAEKLKGMLPAFDLCCYEMTIKWMEMIGDERNSCEIDVWPSLQTLTSDVISRTAFGSSYQEGQKVFKLQMEQADLAFKALQPVYLPGFRFIPTKRNKRMKEIAREVRVLLGGVIEKKMKAVEAGEDSSDDLLGRLLEANSKEVEQGNKTVGMSIEDVMEECKVFYFAGQDTTSVLLVWTLVLLSMYPDWQDRARQEVLQVFEDGKADIGHLNHLKTVTMILYEVLRLYPPVAALIRQINEEITLADMTLLPGMQIVLPINQIHQDHDIWGADAKEFNPERFSEGVSKATKNQVAAFFPFGAGPRICIGQNFALVEAKLAMARILRKFSFELSPSYKHAPVDKITLQPEYGARLILHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AHC5</accession>
    <name>A0A162AHC5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5">5.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_009837</name>
        <name evidence="9" type="ORF">DCAR_0311104</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.-" evidence="5"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AHC5"/>
    <dbReference type="STRING" id="79200.A0A162AHC5"/>
    <dbReference type="Gramene" id="KZN01083">
        <property type="protein sequence ID" value="KZN01083"/>
        <property type="gene ID" value="DCAR_009837"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212225"/>
    <dbReference type="OMA" id="DMGVMIL"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF50">
        <property type="entry name" value="UDP-ARABINOSE 4-EPIMERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="7">
        <location>
            <begin position="53"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN01083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG91849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="396" mass="43407" checksum="C439C1FD5FB73F6F" modified="2016-07-06" version="1">MDIMDSRRRKKFSGKVMLTVGVATLFIIYFKSSSKVTSPKQFSVHEPGVTHVLVTGGAGYIGSHASLRLLKDSYRVTIVDNLSRGNMGAVKILQTLFPEPGRLQFIYADLGDANAVNKIFADNAFDAVMHFAAVAYVGESTAEPLRYYHNITSNTLVVLEAMASHGVKTLIYSSTCATYGEPKVMPITEETPQVPINPYGKAKKMSEDIILDFSKTSKMAVMILRYFNVIGSDPEGRLGEAPPPELREQGRISGACFDAARGIIPGLKIRGTDYSTADGTCVRDYIDVTDLVDAHVKALAHALPGKVGIYNVGTGKGSSVKQFVEACKKATGVPIKIEYLSRRPGDYAEVYSDPSKIKRDLNWVARYTNLEESLKTAWLWQKSHRNGYGSPMITSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AHC8</accession>
    <name>A0A162AHC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_009675</name>
        <name evidence="3" type="ORF">DCAR_0310920</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN00921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AHC8"/>
    <dbReference type="STRING" id="79200.A0A162AHC8"/>
    <dbReference type="Gramene" id="KZN00921">
        <property type="protein sequence ID" value="KZN00921"/>
        <property type="gene ID" value="DCAR_009675"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213744"/>
    <dbReference type="OMA" id="ICDEEDY"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029471">
        <property type="entry name" value="HNH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052892">
        <property type="entry name" value="NA-targeting_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877:SF2">
        <property type="entry name" value="OS07G0170200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877">
        <property type="entry name" value="SLL1193 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14279">
        <property type="entry name" value="HNH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="1">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN00921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG91670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="265" mass="30102" checksum="29CE8DA2FAE8844F" modified="2016-07-06" version="1">MAQLIRAQCRGKPLFNGEATLIGFEPKSKIRFVSTTTTKLLSSASSSVSSSPDDYKFREDVSNVDDSGFCNVGYDLFFDDPEFGRDELSCFRGLVLDISYRPVNVVCWKRAICLEFMDKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIRSTLSRKNVLARDNFTCQYCSSSENLTIDHVVPTARGGEWTWENLVTACSRCNNRKGQKILEEANMKLKKIPKAPKDYDIIAIPLTNSAIQMLKMGKQTPEEWHEYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162BAP7</accession>
    <name>A0A162BAP7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3 8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_004749</name>
        <name evidence="13" type="ORF">DCAR_0105169</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3 8"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN12093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162BAP7"/>
    <dbReference type="STRING" id="79200.A0A162BAP7"/>
    <dbReference type="Gramene" id="KZN12093">
        <property type="protein sequence ID" value="KZN12093"/>
        <property type="gene ID" value="DCAR_004749"/>
    </dbReference>
    <dbReference type="OMA" id="FGAEGNE"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000008">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000012">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000017">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000022">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF11">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="61"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="177"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="294"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="486"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN12093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG85976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="576" mass="63936" checksum="324F88B91F573BDA" modified="2016-07-06" version="1">MDEDFEMPEGMNGAEDDGFMDDGEEEAVSFMKEGDETEIGTEGLKKKLIKEGQGFDSPDPGDEVQVHYTGTLLDGTKFDSSRDRGEPFKFTLGQGQVIKGWDLGIKTMKKGENALFTIPAELAYGESGSPPTIPPNATLQFDVELLSWTSVKDICKDGGLFKKIIKEGEKWENPKDLDEVLVNYEVKLEDGTVIAKADAVEFTVQDGHLCPALAKAVKTMKKGEKVLLTAKPQYGFGEKGKPASGDAAAVPPNASLQITLELVSWKTVSYVGDNKKIIKKILKEGEGYEKPNEGAVVKVKLIGKLQDGTVFLKKGHDDEEPFEFQTDEEKVIEGLDKAVMAMKKGEVALLTIAPECAFGSSGSQQELAMIPPNSTLYYEIELVSFVKEKESWDMNSEEKIEAAGRKKEEGNAWFKAGKYAMASKRYEKAAKSIEYDTSFGEEEKKKAKALKVSCNLNNAACKLKLKDYKQAEKLCTKVLEIDSKNVKALYRRAQAYINLVDLDLAEFDIKKALEIDPANRDVKLEYKVLKEKVKEINKKDAQFYGNMFAKLNKLEPFDTEKTAPKEEEPMSVDSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SPX2</accession>
    <name>A0A164SPX2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_023582</name>
        <name evidence="3" type="ORF">DCAR_0727052</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SPX2"/>
    <dbReference type="Gramene" id="KZM86448">
        <property type="protein sequence ID" value="KZM86448"/>
        <property type="gene ID" value="DCAR_023582"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM86448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="115" mass="12827" checksum="2C25C0AEC476E8D4" modified="2016-07-06" version="1">MHSGAVVTGVNGSLKRASVDTRSERGIGKVSLGTELTDDDDDFETPAEHFGKTKSIRMQKKPRMMKGIRSDNQKSLLNKLRVVYGQKILTWEHNSKKEYVMGGATYLNKGKKIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TBS5</accession>
    <name>A0A164TBS5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_024450</name>
        <name evidence="7" type="ORF">DCAR_0727674</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TBS5"/>
    <dbReference type="Gramene" id="KZM87316">
        <property type="protein sequence ID" value="KZM87316"/>
        <property type="gene ID" value="DCAR_024450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF37">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR VRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM87316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH08236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56513" checksum="C439EB3522B053C8" modified="2016-07-06" version="1">MFLRNSAQNTHFELKGGEYILFEYFGHFTIGDGGWRFVICGVEGDGVIDEIDPPAGFIDRCGFALPHRIMFVLSNGKMFAGSYNSEACRFSGLSSMFRILGIDSFNGVSKFLFTYDGTEFVSQEHLLLLGSDINFSCTLCWCEGMESSGLTLPNFIKFLTNVDTKSDYLKLPTGIVSQYGGRLQECFLMKFRNGYELPVYFSDDQWTISGVSNLYEDFGLNGGEMLLFEFNGKSDFNVYVIGSNLTEIEYPNIVHYLQKNRPRVVSVRNGGLKFVTFVNEDNPMSDEFEPPSSFKRATPMIRGYQNFLFSNGKKIEGGYHHDTGIFHGFKKFCLILGIEDFSNYNVVLFTYEHDKTTKVSVYDDYFVEGLFPRSPVLAGLNSHNPTVFGRIEIYVKPFHMFKYFYEVDISTVYERITAYWRRRDYITAYLGDRAWKLEVRARNRKCQRKTILEGWIQFREDLGLAVGDLLILECPTNSRHHFSLQVIKQPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TW40</accession>
    <name>A0A164TW40_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0728852</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TW40"/>
    <dbReference type="Gramene" id="KZM88051">
        <property type="protein sequence ID" value="KZM88051"/>
        <property type="gene ID" value="DCAR_025126"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193556"/>
    <dbReference type="OMA" id="DTHWIMA"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF113">
        <property type="entry name" value="CARBOXYLESTERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="327" mass="36395" checksum="0ABA24F2D1592A44" modified="2016-07-06" version="1">MERDPSLIDEPYQFLKLVVNADGSLTRGFEFPRVPPTPQLASESPDSSSSEKQLVLSKDIPLNTTNQTFLRLFRPLHHTTKLPVIVDFHGGGFVLMSAISAPFHDMLNRAAVFAPALIITVEYRLAPEHRLPAAYDDALEAIMWVRDQAREVDGSDPWMKELADYSNVHIMGTSAGGNIAYHAGLRVLDTDISPIQVKGLILNQPFFGGVQRTPSEERLSRNPYLPVTVSDLLWLLSLPPGSDRDHEYSNPLDVVNPKIQRLPRCLIRGFEGDALVDRIKGVAKMLEAHGVKVVSKFEEGGFHGAEMADPVKIQELFLQIKDFVYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UUL3</accession>
    <name>A0A164UUL3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0622676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UUL3"/>
    <dbReference type="Gramene" id="KZM89381">
        <property type="protein sequence ID" value="KZM89381"/>
        <property type="gene ID" value="DCAR_023256"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226408"/>
    <dbReference type="OMA" id="TGHFYIG"/>
    <dbReference type="OrthoDB" id="1882431at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000041">
        <property type="entry name" value="Basic 7S globulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF68">
        <property type="entry name" value="BASIC 7S GLOBULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH03280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="444" mass="48389" checksum="325CE2E1B33C636A" modified="2016-07-06" version="1">MSSSIHHYAVIFLCLAAAIFSVSSAATFKKPKPTAFLFPIRKDTKTLQYYTTLDISSRENSVQLVLDLGGQHTWFTCNAEVLASFKAVDCHSRKCKDYKSTSCMRCGSDPAVNVGCTDDACAVDYRNPFNTREIGRGLGEDALFVDSTNGLSVGFNYQSPKPFPFSCLESDEYLIGGLSNETKGMIGLMNTTTSLHAQLSTQFKIPHKFALCLPSTSDFILGHMFIGGKPYFYPPYNKDIGRELITAKLVRYPVSTANVFSVGDPLDEYFIDVKSISVDHKIVAFNASLLSINEEGTGGTTLSAVTPYTTLVTPIYESLLSAFTKAAALRKMKKVSAVAPFGACYKAKSVAKSQTGPVVPYIDIGLTGGKQHWRFYGANSMVLVKKDVLCLAFVDGGSSVRTSVVIGGHQMENYLIEFDLVTPKVGISTSLLFRNSTCSQTRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UY00</accession>
    <name>A0A164UY00_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0622908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UY00"/>
    <dbReference type="Gramene" id="KZM89527">
        <property type="protein sequence ID" value="KZM89527"/>
        <property type="gene ID" value="DCAR_023110"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227608"/>
    <dbReference type="OMA" id="PNDYVES"/>
    <dbReference type="OrthoDB" id="1892230at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021099">
        <property type="entry name" value="PORR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045040">
        <property type="entry name" value="PORR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476:SF16">
        <property type="entry name" value="F14O23.23 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11955">
        <property type="entry name" value="PORR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH03509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="711" mass="82528" checksum="E6EE9BDE897505C3" modified="2016-07-06" version="1">MKILLHILQSPAYRRHQTRSIFDSAAPIKWVRDRALDHAVEKERNLKPMINLKNFIKSEPSKTSPISLILQHKHDLETPFRPIEFIRKYPSVFEEFLPGGIGVNPHIRLTNEVMQLDGEEEMVFLSGGHKQDVADKLLKLLMVGKVNRIPLRVIDKLKWDLGLPNDYVESVVPEFPDYFCVRKCDGLGVLELVCWSEELAVSEMEKRAMSGKEGYSKGMPIAFPLQYSRGFEVDKKFKKWVDDWQKLPYVTPYESVLSCQGKGDEFDKWAVGVLHEILSLFVPKKTEKDNILLLGDYLGVRSRFKKALLQHPGIFYISSKLHTHTVVLRDAYKRDLLIGKPNPVMEMRSKYIHLMTAVKEESKSKSAQVGNTRRKKKSVDLKEGEEEDSKDDENEEEEDENSNRLSDSEFEEESDDDDYEDESDDDESEHESTMNRGRASGKSSFEEKARALRTEQKQFDGRHPSKTGDRRSPTHSRGKEIPAKFDRDESNRRHQSKTLDGQSPSYERGRQSPIRNERHESTGRRESNYRHERDESKRSYPDKAQDKRSSTFSGRREGPWRDESDRSARRQSPIRNEREESNGRRESNFRNDSNESKRSYPDKSQDKRSSTFSGRRERPWRNEPDRAAKRYPRGTEDSQSPQSFRGQEASPTNSPNQSDRRHPSKARDEQSPTHTRGKETLKNQMARRKPHGRSDVSRTRKSTSPNKTPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VUI3</accession>
    <name>A0A164VUI3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_021761</name>
        <name evidence="13" type="ORF">DCAR_0624444</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VUI3"/>
    <dbReference type="STRING" id="79200.A0A164VUI3"/>
    <dbReference type="Gramene" id="KZM90874">
        <property type="protein sequence ID" value="KZM90874"/>
        <property type="gene ID" value="DCAR_021761"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227595"/>
    <dbReference type="OMA" id="ESEAYQD"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05506">
        <property type="entry name" value="Bromo_plant1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR052442">
        <property type="entry name" value="Env_Response_Regulator"/>
    </dbReference>
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        <property type="entry name" value="GTE_bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="175"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="11">
        <location>
            <begin position="309"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="429"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="494"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="700"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="580"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM90874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH05032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="79357" checksum="CA37D478961CAE73" modified="2016-07-06" version="1">MDKRNRFPEGDSSSDESGSSGRIDTEISASENCSAPRKKWIMLNPATKDGFGVPVKVLPLSKLSHPDRQKLLLCLKTELEKVGFLRKKVETRNVGVVSVSPSTSILSGSNAQNVLKNGNTKRSSALTDAPAKKLKPGSLTRGTSGRFQSSNKPSEPSSMNSTLMRQCENLLKKLMSHEFGWVFNSPVDPVKLNLPDYFDVIKNPMDLGTVKKKISSSVYTSPLDFVADVRLAFTNAMTYNPPANDVHLMAATLSKFFEVRWKVIEKKLSANVPQQMPQISSVPTEAGKVKPMPPSKKRKHSSTQLLVLPEPVCEPVKCMTAEEKQKLSKELESSLSDLPDNIIEFLREQSSAGKDEGEDEIEIDIDTLTDDTLFKLQKLLDDHLTEKRKHGKGEPCEIELLNESGVSNSSMQLDKGNGLVAKDVDLVGNEPPVSSYPPTEIIHDSGSRSNKFISPGTSSDSDSRSFPAMAADVATASSSPVKRSKDDTVHVAELDENAGPGDLVDGNQSVSGLDQLEPSQQKPSSVESDSCQDGDSAPSERPVSPDKLYRVAILKKRFADTILKAQEKTLKQGEKVDPERLRLEREMLESQKRKEKARLQAEAKAAENAMRLAEAEAAAESKRQRELEREAARQALLKMEKTVEINGTSRFLEDLEMLRTVPSEQVPSSVDEASPDESLDCFKFEGSNPLEQLGLYMKVDDEDEEIEPPSNDVDVEEGEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X3Q4</accession>
    <name>A0A164X3Q4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_019971</name>
        <name evidence="3" type="ORF">DCAR_0626509</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM92664">
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        <property type="gene ID" value="DCAR_019971"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF228">
        <property type="entry name" value="SAUR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM92664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="80" mass="9550" checksum="E9206D7ADEA7CD27" modified="2016-07-06" version="1">MENKTKVKKGWIAVQVGLEDEDEIASRFHIPIFYLNNPLFRELLDKAHEVYGYHISGPLRLPCSVDEFVHLRWQIERKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XKC9</accession>
    <name>A0A164XKC9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_016531</name>
        <name evidence="10" type="ORF">DCAR_0518893</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XKC9"/>
    <dbReference type="Gramene" id="KZM93286">
        <property type="protein sequence ID" value="KZM93286"/>
        <property type="gene ID" value="DCAR_016531"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222062"/>
    <dbReference type="OMA" id="KAMPYIV"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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        <source>
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    <sequence length="598" mass="66890" checksum="F79E2B6727AC50A8" modified="2016-07-06" version="1">MDKSPRVIPESQKDDDETMRSEPNYRGIKAMPYIVGNETFEKLGTIGTSSNFLVYLTSVFHMKTITATNLNNIFQGTCNFGTIIGAFLSDTYFGRYKTLGFASISSFTGMLFLTLTAAVSKLHPPSCGKEETSICQGPTTGQMAFLLSSFALLVVGGSGIRPCNLAFGADQFNPNTDSGRKGINSFFNWYYFTYTFAVMVSLTIIVYVQSNISWSIGLGIPTLLMFFSCVFFFVGTNIYVIVLPQGSPLLAVVQVIVAAIKKRGLESPEQPWLTLFDYIPKSSTNSRLSYTDQFRFLDKAAVRTLEDELKIDGSAADPWRLCTIQQVEEVKCLLRLVPIWFAILIYHVAFIQQNNYLVFQALQSDRRLIKSSTFKIPAATYIVFTMITLTIWIPIYDRIIVPFLRKFTGKEGGITVLQRMGIGIVLSSLSLFVSALIESKRRTWALTRPTLGIEPRKGTISSMSGYWFIPQLIVAGLSEGFFMIGQNELFYKQSPENMKSIATSCLFCASAVSSYLSSFMQTMVQKMTEGKGKPGWLAEDLNQGRLDYFYYIIVALEALNLAYFIICGKWYKYKTASKDTTPDPGVALEELKSEKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A420</accession>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="9">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007608">
        <property type="entry name" value="Senescence_reg_S40"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46525:SF17">
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    </dbReference>
    <dbReference type="Pfam" id="PF04520">
        <property type="entry name" value="Senescence_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG96505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="16967" checksum="50A2C48AED5E90D8" modified="2016-07-06" version="1">MSTKKNYLYFGSEKINPVSSDFEFDESDVWNTGQGVSSEPKRSFFGARAPKKPAKRHDHCNDHATDVSSRSLPVNVPDWSKILGSAYKNRTVEIDDENEGEDGDERVPPHEYLARTRGASGSVQEGAGRTLKGRDMRMVRNAIWKQTGFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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    <name>A0A165YTV0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="3 4">
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KZM99235"/>
        <property type="gene ID" value="DCAR_013403"/>
    </dbReference>
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    <dbReference type="OMA" id="SHDEFLC"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="127" mass="14313" checksum="2FE5DD71B81F3766" modified="2016-07-06" version="1">MLGKKFYAVKKLAKKVQSMKKVDSEQSQRHSLLKDSGDHEDDQYSTPTGFLAVYVGEDRKRFVVPTGYLSHPLFKMLLEKAYKEFGFKQMSGLVVPCSVAAFQEVISAVECCNGNFDFGNLVEEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z8F2</accession>
    <name>A0A165Z8F2_DAUCS</name>
    <protein>
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        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apioideae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
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        <subcellularLocation>
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            <topology evidence="1 11">Single-pass type II membrane protein</topology>
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    </comment>
    <comment type="similarity">
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    </comment>
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    <dbReference type="EMBL" id="CP093346">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="probable pectin methyltransferase QUA2"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="PECTIN METHYLTRANSFERASE QUA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG98859.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AN43</accession>
    <name>A0A166AN43_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0311715</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AN43"/>
    <dbReference type="Gramene" id="KZN01530">
        <property type="protein sequence ID" value="KZN01530"/>
        <property type="gene ID" value="DCAR_010284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="10992" checksum="29E59FA6795943BE" modified="2016-07-06" version="1">MDRVVKHIAGTTTTNILNEMKKSPSGAFISDLYGKIVGKEISVKIDLTEANLNGDNNIYEAVDIFDPSVQQRGAVEVVHGIELFQMPSSSNLYPRRLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166B104</accession>
    <name>A0A166B104_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0312450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166B104"/>
    <dbReference type="Gramene" id="KZN02210">
        <property type="protein sequence ID" value="KZN02210"/>
        <property type="gene ID" value="DCAR_010964"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212550"/>
    <dbReference type="OMA" id="HIFIPLC"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48065">
        <property type="entry name" value="OS10G0469600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48065:SF5">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN CF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG93169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="841" mass="93990" checksum="DC2E0D58E42B4D83" modified="2016-07-06" version="1">MKIQLLTSLLLVPLFHILFGLDIVLVLGQCLDDQKFFLLQFKNSVKFNSTLSSKLVSWDQSTDCCTWEGVTCNLSSNRGHVIGLDINSESIYGCLVKSTSNSLSGLRHLESLNLAFNNFDTSKFPSGIFSLTALTYLNLSIAGFRGNFPSSIANLTQLVHLDFWANNFSGIISSTHFQNLVNLEYLDLGLNLLSGTIPSSLFGLPSLRKLFLSYNNFQGSVPKFTNTNLSPLETLYASWNNLSGPVPISLFDLKNFSRLTLSHNKLSGKFHLQILHRLSNLTYFNLSFNNLSVDLGHDNSSKFLPPSLKELKLASCRLQHFPELRYLPSLITLDLAENQISGAIPNWIWNVGNGGLRYLNLSVNQLHKLQEPYVIHNLSSIDLRCNHLRGEIPIPIENIYFLDYSNNFFSSIPTNFNLTSAIFFSVSSNRLSGTIPVSMCSGTNLQLLDLSNNYLIGNIPSCLFELSKSLGVLKLGNNSLTGNISRIFSNDCGLETLDLHGNLLKGSVPESLANCSKLEVLNLGDNRINDRFPCFLKDSLHLHVLVLHSNLFHGEIRCQEKNGSWINLQIYDIGSNHFTGNLSQNFLIWKAMKDGRNDVNILGFEALRFGGSRKTYQDAVTISIKGKRLDLVKILNIFTSIDISNNHFQGNIPHTIGELKSLVSLNLSHNTLSGSIPNSFGTLKLLETLDLSENKLTGKIPMELGDISFLEVLDLSNNQLTGKIPTGRQLQTFPEASFEGNKELCGPPLHIKCSRTAVTTAGYGDGDLGNSINWEFVGPEVGFVVGLGTVILPLIFYKRWRSYYYEHVDHILFKVIRRKHQKKSIHGRIRSRYSRIERARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DAI4</accession>
    <name>A0A166DAI4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206576</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DAI4"/>
    <dbReference type="Gramene" id="KZN05009">
        <property type="protein sequence ID" value="KZN05009"/>
        <property type="gene ID" value="DCAR_005846"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208233"/>
    <dbReference type="OMA" id="NEIMSHR"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000028">
        <property type="entry name" value="Glutaredoxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="104" mass="11375" checksum="3F692051C7FEA316" modified="2016-07-06" version="1">MEKISRLVSENGVVIFSKSTCCLCYAVDILFHELEVRPVVYEIDKDPEGREMEKALYRLGCSAPAVPAVFIGGKLVGSTNEVMSLHLSGSLVTLIKPYQAVLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EC44</accession>
    <name>A0A166EC44_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein SPIRAL1-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_007199</name>
        <name evidence="5" type="ORF">DCAR_0208123</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EC44"/>
    <dbReference type="STRING" id="79200.A0A166EC44"/>
    <dbReference type="Gramene" id="KZN06362">
        <property type="protein sequence ID" value="KZN06362"/>
        <property type="gene ID" value="DCAR_007199"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206242"/>
    <dbReference type="OrthoDB" id="62622at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010005">
        <property type="term" value="C:cortical microtubule, transverse to long axis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN06362.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG88888.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="10507" checksum="F3B247BDCC421560" modified="2016-07-06" version="1">MGRGVSSGGGQSSLNYLFGSGEAPKPSTKDAEASLNDAQAETHSPKPSTPPKSVDVSKQIPAGINSTATSGQNTGNFLTDRPSTKVHSAPGGGSSLGYLFGGNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Gramene" id="KZN06466">
        <property type="protein sequence ID" value="KZN06466"/>
        <property type="gene ID" value="DCAR_007303"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN06466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG89007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="331" mass="36481" checksum="609DA999BE919712" modified="2016-07-06" version="1">MELHWEIPEGGYKKINVHCVILDAPLPNGNQVSIASLIRDSTGAKLWGALGPLPLQSEEQAIMTGIQAALIHAQEKGWELTHIETTSMQVYDTVTHQEHFFLDDVQLEAYGAFNTIYANHYEKDRTKRVIACIPARMNGSAAYMANYGLNHASEFGEISGLVGELEYYLARDMGMTLPRPTLEVALNIGDGEVIDGPPPSKKRKCAQQDAMQKRVFKDKGKDKVYEHFSFNDNGLISSRALHALESNHLSDHSPEFSKDVINLDAPVGTGIYARDVLHHAVTGSLQSIIPKLYVSNPFSRDFDLKKLMSVDKVLALMGFTKGKASSSAGPV</sequence>
</entry>
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        <name evidence="3" type="ORF">DCAR_007339</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN06502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EFR0"/>
    <dbReference type="Gramene" id="KZN06502">
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        <property type="gene ID" value="DCAR_007339"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN06502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11981" checksum="00B15407E2B3B555" modified="2016-07-06" version="1">MLKSPNNVLVILVAFLFVFLMIISNEACRMLDEDAEQLKTTTTKSSCHYHDFHLLQSLQRGSVPPPGRRNGCTFIPGKSKTPCKIDGQGFAGRPSRRSATPAFMHEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FM55</accession>
    <name>A0A166FM55_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine racemase</fullName>
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            <ecNumber evidence="10">5.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">D-serine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_000604</name>
        <name evidence="15" type="ORF">DCAR_0100382</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the serine/threonine dehydratase family.</text>
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    <dbReference type="EC" id="4.3.1.18" evidence="9"/>
    <dbReference type="EC" id="5.1.1.18" evidence="10"/>
    <dbReference type="EMBL" id="LNRQ01000001">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG81237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166FM55"/>
    <dbReference type="Gramene" id="KZN07935">
        <property type="protein sequence ID" value="KZN07935"/>
        <property type="gene ID" value="DCAR_000604"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201032"/>
    <dbReference type="OMA" id="LIHPFDH"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
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        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030378">
        <property type="term" value="F:serine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018114">
        <property type="term" value="F:threonine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070179">
        <property type="term" value="P:D-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006563">
        <property type="term" value="P:L-serine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050">
        <property type="entry name" value="SERINE / THREONINE RACEMASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050:SF1">
        <property type="entry name" value="SERINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN07935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG81237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="332" mass="35505" checksum="CC27F2E2BF9C43B2" modified="2016-07-06" version="1">MEVNTERYGEIYGVDISAIKEAQVRISPFIHTTPVLSSETLNSTAGRKLYFKCECFQKGGAFKFRGASNAVLSLDNDQAAKGVVTHSSGNHAAALSLAAKLRGIPAYIIIPKNAPNCKVENVRRYGGHIIRSEATMQSREETASRVLQETGAVLLHPYNDKRIISGQGTISLELLKQAPLIDTIIVPISGGGLISGVALAAKSINPNIRIFAAEPRGANDAAQSKKAGRIITLPETNTIADGLRASLGDLTWPIVRDLVDDIIVVEDKEIIQAMRLCYEILKVAVEPSGAIGLAAVLSDSFKGNPAWKDCHHIGIVLSGGNVDLGTLWDSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G4R8</accession>
    <name>A0A166G4R8_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZN08532">
        <property type="protein sequence ID" value="KZN08532"/>
        <property type="gene ID" value="DCAR_001062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="191" mass="22705" checksum="6630976D76D33592" modified="2016-07-06" version="1">MQSLSPEQKNSTKPISEPGNVWEKIAPTYSRASQCAFSRLDFDSKQLQNAKRGCIQGMCTAKLMFWTRTNSFLFEYPFHVQGPIHKTFNTVRHLFTWNFNICDRWRGFGMHTIYFWKLSSCVYPDPASMPTLIWFKLCVRILALAALLTNFILLFVFRRGAIARWVFHRLSKHNWNSYWRTFKFRQQSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H908</accession>
    <name>A0A166H908_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_002613</name>
        <name evidence="22" type="ORF">DCAR_0102738</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H908"/>
    <dbReference type="Gramene" id="KZN09957">
        <property type="protein sequence ID" value="KZN09957"/>
        <property type="gene ID" value="DCAR_002613"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213298"/>
    <dbReference type="OMA" id="HVNFRYN"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000716">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000416">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At5g63930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF81">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007874462" description="non-specific serine/threonine protein kinase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="19">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="20">
        <location>
            <begin position="194"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KZN09957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG83562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="587" mass="63695" checksum="511906DB07D28643" modified="2016-07-06" version="1">MQILMWVFKFLILVSFFHLSLQAPPICSETDRAALLDFKGRIFKDTTGILASWVGLDCCDGGWEGITCNPRTGRVTQVVLQRPADGDSGVFMKGTLSASLGKLRFLEVMIISGMKRIAGNIPQSFSGLTSLSQLHLEDNALEGNIPSSLGDLPYLRALSLSGNRLTGPIPPTFGKYKNLVQLTLARNYLEGPIPTSLKSLFSLQYLDLSRNTLSGFIPDFVGQFKNLTFLDFSSNHLSGAIPISLGGLGSLSDMSLSQNQLTGSIPNEIGHLKSLTSLSLSMNQLSGQIPDTLSQLQNLWHLNLSGNALSNPLPDALSKGIPSLLSIDLSYNRLNLVRVPEWIRNRELSDVNLAGCNLIGALPVFAKPNSLSSIDLSNNHFTEGLSNFFSKMTSLQKAKLSNNQLKSDISAITLPAGLASLDLHSNQLFGSLSSLLNRTSNFMQAIDLSNNYISGSIPEFSEGSSLTLLNIGSNKITGQIPDSISNLVTLERLDISRNQVTGTIPTSLGLLLKLQWLDLSINKLGGKIPVSLLDIEKLRHANFRANKLCGMIPQGRPFNAFPATAYGHNLCLCGKPLPPCKKMDKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HT45</accession>
    <name>A0A166HT45_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Folate-biopterin transporter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_003015</name>
        <name evidence="10" type="ORF">DCAR_0103169</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Folate-biopterin transporter (TC 2.A.71) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HT45"/>
    <dbReference type="Gramene" id="KZN10359">
        <property type="protein sequence ID" value="KZN10359"/>
        <property type="gene ID" value="DCAR_003015"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204228"/>
    <dbReference type="OMA" id="MTGRLKW"/>
    <dbReference type="OrthoDB" id="754047at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17484">
        <property type="entry name" value="MFS_FBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039309">
        <property type="entry name" value="BT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31585:SF6">
        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN10359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="54963" checksum="CE7282332B3C1C7F" modified="2016-07-06" version="1">MVEEELPISSEAEEVQHYEPKKGISGCFWGPINWFRVLCVEMHWSFVFGVLMVYGVSQGLGGALARVGTEYYMKDVQKVQPSEAQVYSGITNIPWLVKPLWGLLTDVVPVFGYRRRPYFIFAGIFGLISMLFLSLHKSLHIAFALLSLLVGSTGVAIADVTIDACVAQKSGSHPSFAADMQSLCSLSTSIGALVGFSISGIFVHLIGPRGVYGLLTIPAGLLLVVGFVLKEPPIHNFAYGQVHVKFQDAASSMWNTLKYPDVWRPCLYMYLSFALSLNIYEGMFYWYTDSKDGPTFSKEAIGYMLSIGSVGSLLGAFMYQYGLKDHPFRNILFWAQLVLGLSGMLDFVLVLRLNLKFGIPDYLFAVIDESIYRMTATLKWMPLLVLSSKLCPPGIEGTFFALLMSIDNAGLFSSTWGGGLLLHLMNVTRTRFDNLWLAILIRNVLRLAPLTLLFLVSRGDPASSILPNEPHDAKAVEDVPDSKDIELVSLVNSADGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IDU1</accession>
    <name>A0A166IDU1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF1640 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_003681</name>
        <name evidence="10" type="ORF">DCAR_0103901</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IDU1"/>
    <dbReference type="Gramene" id="KZN11025">
        <property type="protein sequence ID" value="KZN11025"/>
        <property type="gene ID" value="DCAR_003681"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210699"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000029">
        <property type="entry name" value="Coiled-coil domain-containing protein 90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN11025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26428" checksum="E8805C42D223F057" modified="2016-07-06" version="1">MAACKRVFQLGSNSAICLSKLSKFELAKSKLYGTAAAASNSSANQLSYRSISQHVNLNGSRAFLVDTLALVRRLEAQGVPSQQAEAITAAITEVLNDSLENVSHAFVSRAESQKGEMLQEANLSKFKAEIQSSQEHHFSLLQRETEKLQNDIGKIRSELKYEVDKVTAGQRLDLNLERGRTRDELANQSTETTNLNNKIDREIQGIRAQLEGAKYDIIKYCIGTLVSVSAVGLAVLRILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166JDA4</accession>
    <name>A0A166JDA4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0105112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166JDA4"/>
    <dbReference type="Gramene" id="KZN12042">
        <property type="protein sequence ID" value="KZN12042"/>
        <property type="gene ID" value="DCAR_004698"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208225"/>
    <dbReference type="OMA" id="KVPWRQM"/>
    <dbReference type="OrthoDB" id="5130at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021883">
        <property type="entry name" value="LPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498:SF1">
        <property type="entry name" value="LOW PSII ACCUMULATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498">
        <property type="entry name" value="PROTEIN LOW PSII ACCUMULATION 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11998">
        <property type="entry name" value="DUF3493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="320" mass="35419" checksum="FB454CB7FA28568F" modified="2016-07-06" version="1">MSVIITSSFGHHPKLSALSRQIIPENSVYIFPTVTITISPIQLRRKGLRFSLTCFASDKPSPSPQFSTDAKIRSEVLSPFRSVRMFFYLAFIASASLGGLIATTQLIGALSNPSRADEVTKILEGLGIDISAVSVCAFLYSRDNNAKNAQLARLTREENLSNLKLRVDEKKVIPVGDFRGIARLVLVSGPSSFISKSFKLSEPFTESLLERGVLVVPFATDGELPDFEFDDSEDMKELTERRKRLWQLKPVFSTEWSEWLDEQKKLANVSTESPVYLSLRLSGRVRGSGVGYPPWNAFVAQLPPTKGLWSGLLDGMDGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WTB6</accession>
    <name>A0A169WTB6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Peroxidase</fullName>
            <ecNumber evidence="3 17">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_002515</name>
        <name evidence="20" type="ORF">DCAR_0102621</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14 17">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="14 17">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 17"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WTB6"/>
    <dbReference type="STRING" id="79200.A0A169WTB6"/>
    <dbReference type="Gramene" id="KZN09859">
        <property type="protein sequence ID" value="KZN09859"/>
        <property type="gene ID" value="DCAR_002515"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194950"/>
    <dbReference type="OMA" id="VMDPRTA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000007">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF81">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 17" id="KW-0349">Heme</keyword>
    <keyword evidence="17" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 14 17" id="KW-0408">Iron</keyword>
    <keyword evidence="6 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 17" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007749573" description="Peroxidase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="18">
        <location>
            <begin position="26"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="15">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="121"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KZN09859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG83446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="321" mass="34970" checksum="888500075DF4EE40" modified="2016-07-06" version="1">MLFSVLIFIFFSFNLNTELLEAKGPSLSYGFYKDSCPQVENIIRAALSSIHLMDPTSAPSLLRLLFHDCQVQGCDASILLDSTNGGTPEIISSKNFGVRKLEHINYIKSMVEAACPLKVSCADILVLAAREAVALSLGPRIPVPLGRRDSAVAPSSKAADTLLPPVSINVDGMLQLFAGKGMTVEEAVAIMGAHSLGVTHCVNILDRLARVDKNMSPGFEVSLRLSCPFGSLTPNTSFVLNDPTTLMFDNHYYWSTLRGRGVLKIDADLATDPRTLPFVQRFAANEGEFFRAFSSAFVKLSSYGVLTGKQGMIRRSCNRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YAI1</accession>
    <name>A0A175YAI1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0414431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YAI1"/>
    <dbReference type="Gramene" id="KZM80596">
        <property type="protein sequence ID" value="KZM80596"/>
        <property type="gene ID" value="DCAR_032053"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="933"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="939"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="971"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1047" mass="120107" checksum="2EB4CFB115ADF3F1" modified="2016-09-07" version="1">MSKPSSSSHKFSLIKELELEKSEFEKEVDLLFEEMNLHLETETERIKKEKLEKEMEEILVYKKQLEDLIEGAKLPRNEVWEKLLQIVWEIFDSRIYDIEITISHTKSVHFMHDSRMPDNKFQRTAKNKGTDEDSSVKFEIDGKEHIQTVAKINKKFGNTLANDESYRDLADDATLTKFFLKIGYDGLVLKEDSTDWYPTGEMDRKYIRKEWNMIFDAMVKIFSAKTSGWNAIPSYIRNLTHSMVHGYKVNVGNMIMAQLRSSIIRKVQIYPRFVTMFLNDVCGIAANASNTRKCFVLKKNTHNSLINSNPHGDMRLHYTRHMIDQVSNLDSPFEDNSVIFSLEDEAGKHTFEKPALEETHIGLRHSEISSPMTIDEPQLTSLTQPPIISEILSSTCPTSLVSSLTPRAEDCPNLSDVDLPFQIMEEPPSSGPQEPIAQSTTTSIAVDSIPTVKTSLVVTSTPAGKAHTAVFTQTDSIVSTQPVVTSMQHPSTVLVEDDSDDDHVLISSFIKATLDTLSKLINAANLPKGENSDRSDERELDDEPVKGVASGTSKGNQGASAQGEQGEATKEDLSKERFGEREASTQGEPGTVEMIIDGERVLASKTLAEELNSSEDARRNVFVSRTIFPPLRMREPTSGSFALERSFKQREEEITKRKGKEKLAEYADEFVKLILDARTGSENILMNWSDNFKEQIADFCVKINEKHIWSVYICLKTIHTLYVTMEVLKQQPAIVLFTLISLMKRKEILVNQILRENLLHLVIEKALEVHMNPFSLFYRTKSAEHSDKYTQMRKREEFKNYIEKRAARWTSRLWSRTKHPANDHFKRLHNKTVETPIQTAEENSFAAMKRLLLCQVIDARVDFDGEVNVISYRLFHPVKQGHVCETVFPIYFFKLERMIEFMMDDPSMSMQTMEPILKYHTLRKREFDKRNGRNWLMPDDSDYPEEEEDVQIDESSDDDDDLTADDEINLSKKTSQGNTSTTELHQYTPPSYQEQREEVINLHRVLNRDRSMKPDHVSRVLDVVSQIFNSDLIIIDLICLDIMVVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGB8</accession>
    <name>A0A175YGB8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein groES</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0935109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Seems to function only as a co-chaperone, along with cpn60, and in certain cases is essential for the discharge of biologically active proteins from cpn60.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms stable complexes with CPN60 in the presence of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGB8"/>
    <dbReference type="Gramene" id="KZM82659">
        <property type="protein sequence ID" value="KZM82659"/>
        <property type="gene ID" value="DCAR_030228"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201875"/>
    <dbReference type="OMA" id="EDFLIMR"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF37">
        <property type="entry name" value="GROES-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH15567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10401" checksum="E300F957C30C0051" modified="2016-09-07" version="1">MAKRLIPTLNRVLIEKIVPPSKTTAGILLPEKSSKLNSGKVVAVGPGALDKSGNKIPVAVKEGDTVLLPEYGGTQVKLGEKEYHLFRDDDILGTLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YI51</accession>
    <name>A0A175YI51_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0935619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH16070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM83087">
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        <property type="gene ID" value="DCAR_030656"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="429" mass="49139" checksum="AFC93D02664C6204" modified="2016-09-07" version="1">MAFATRLYIKSTIPRYNIRACKLNMNGLNASAAIAIPASSQPSNNQTFPNNIQPNRFHALRRRIDLEVMNPYSRCGRIWRKFIISFFISVFLLQTLSIFLQYVKQDKNCIAQIDSRLEGISQILLKLSNIIFILHIIIKFRTAYVDPKSRLLVYEPKKQISTLAGNQTPSVFVVEVLFTMFITAMGLLLFSFLIGNIQRFLQARGSRSFENSLRGSDIEQWMSHRQLPDDLKAKIRESERYNWLATRGLNELMLLENLPEDLQRDIRRHLFKFENKLPIVASMDESILDAIRERMKHNTYIEGSRVLVRGGLMDKMVYIVQGKLESTSEGETVVPLSEGDVCGAELITLCLEHYVLNRDGDKFRIPAGKLVSKRTVRCLTNVEAFTLRAADLEDVFSLYSGLLIQNPLVQGAITKESLHPKSLLRSRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKS0</accession>
    <name>A0A175YKS0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0831160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKS0"/>
    <dbReference type="Gramene" id="KZM84316">
        <property type="protein sequence ID" value="KZM84316"/>
        <property type="gene ID" value="DCAR_028390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH11670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="75" mass="7887" checksum="51AB24AAB8CBA186" modified="2016-09-07" version="1">MAGKVPISQMEPGEALVMRIIEEAKQAVGIETDAGGRQRIIHRQLRTTASVSATSRAPAGSVTNAVPYHVYAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YNA9</accession>
    <name>A0A175YNA9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF241 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_027625</name>
        <name evidence="2" type="ORF">DCAR_0831874</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YNA9"/>
    <dbReference type="STRING" id="79200.A0A175YNA9"/>
    <dbReference type="Gramene" id="KZM84953">
        <property type="protein sequence ID" value="KZM84953"/>
        <property type="gene ID" value="DCAR_027625"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199131"/>
    <dbReference type="OMA" id="DNHEATK"/>
    <dbReference type="OrthoDB" id="1701699at2759"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070:SF109">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (DUF241)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KZM84953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH12371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31684" checksum="A407974C10285C3B" modified="2016-09-07" version="1">MASTSYHVRSISLPCRSHPAIIRVKEELSKLKTLETPCCQTSEAICNSLSGLAELYDCTNDLLGLKLTKQSALMDCLLDGSVRLLDICGGTRDIVSQIKEHTRDLESSIRRRKGDISIESSVVKFNSFRRRMKKEAKRLIASLKQIKKQYEALAMLDSDQKHSSVIGLLTEVTLSSISVYQSLLMFVTVPVSKTKGRRWSAITKLIHTERVSCEEENMNELERIDAALISLCRSRKGEKIESAQRGLVALETHFEGIESNLECLFRRLIKTRTLFLNMASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YP76</accession>
    <name>A0A175YP76_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_027163</name>
        <name evidence="8" type="ORF">DCAR_0832391</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YP76"/>
    <dbReference type="STRING" id="79200.A0A175YP76"/>
    <dbReference type="Gramene" id="KZM85415">
        <property type="protein sequence ID" value="KZM85415"/>
        <property type="gene ID" value="DCAR_027163"/>
    </dbReference>
    <dbReference type="OMA" id="HAFMNEL"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        </source>
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        <source>
            <dbReference type="EMBL" id="WOH12882.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="403" mass="42862" checksum="88D4516F8E5AAF17" modified="2016-09-07" version="1">MASPLTNLIHFFLICTIHFITSSTAKSSTRPKGLILSVYKDPSTLQYITEIKQRTPLVPVKLVVDLGAEFLWVNCEKDFTTSSYKPALCNSAPCKLSKSIACTTECYSPAKPGCTNNTCDLFPENTITLLATSGSLGSDALTVQSGAGSVTVPNFLFVCGSTIILDKLASGVTGMLGLGRTKISMPSQLFSTFGMKRKFGVCLSSTPSKGSIFFGEFDSSTAPLTHTPLLTNPVTTAGVFSKGEASSEYFNGKAVRVNPALLSINNNTGHGGTKISTVDKYTVLEASIYKAVVHAFMNELNVPRVRSVAPFGACFDSKKVGTAYTGPAVPTIDLVLQSKDVYWRIYGANSMVQVSKDVSCLGFVDGGVDSRTSIVIGGHQIEENLLEIDFKASTLGFSPASVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    </protein>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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    </dbReference>
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            <begin position="20"/>
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            <begin position="274"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="34984" checksum="081185EE0D0B1284" modified="2024-05-29" version="1">MSLLVRWRFQTSVNEILSVLRFEFIFIILSIPCVSQSSAFVIKGGTTGGILTGAMLLVIPGGLILLTSFFIVIVIFPGNFVQYKEVRYTDRNRNRMTNLWQLFTGKASTGKWFYGERLPSSIIQRFGIIFENTKGPPIYIVIDQNDPNQMPRWTESGQNGIGSMRALSSDDGIEETIASVSTRHLGCLRSSYVILDLTRRVALGILSGVHSSRGTNQNLYALIITLVQVLYLFTLKPHIRRAVHVVETISLLCEAGVFRLAIITSRTDAADKNTVGYIMLALLFCIAFVSQIINQWYHHKISFEILTASAGLI</sequence>
</entry>
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        <recommendedName>
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        <lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <location>
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        </location>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="470" mass="54237" checksum="7AEBEAA10480590E" modified="2024-05-29" version="1">MYNGSGDGEASASKKIPPATSMLWNRKLRRFIECGTGRALMELETKRTLLDMFKEKQQKNAESGSLPSFYKKKPEDGYISHRVQRLEKCRFKKKQSDLLLNADDLDAMWVCLRQNCVIDDATGAEKMNYEDFCHIASECTEQMGSKCRRFFSPSNFMKFEKDESGRIAILPFYLYVMRMVSLTQVRIDMSELDEDSDGFLQPHQMKTYISGLIPNLAQLRDMPEAFISMYCCIAAHKFFFFCDPHRRGKACIKKLLLSNALQELIELQEESEEEVTDTEQAENWFSLTSAQRICDMFLALDKDMSGTLNKHELRAYADGTLTDIFIERAFDEHIGRGKNGGNGREMDFENFLDFVLALKNKDTEEGLTYLFRCLDLHGRGFLTTADIHTLFRDVHRKWIDGGNYELCIEDVRDEIWDMVKPADPLRIPLADLLSCKQGGTVASMLIDVRGFWAHDNRENLLQEEEEPEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W540</accession>
    <name>A0AAF0W540_DAUCS</name>
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            <fullName evidence="4 19">Peroxidase</fullName>
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    </gene>
    <organism evidence="21 22">
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 19"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="secretory_peroxidase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Haem_peroxidase"/>
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        <property type="entry name" value="Haem_peroxidase_sf"/>
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        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF51">
        <property type="entry name" value="PEROXIDASE 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041768049" description="Peroxidase" evidence="19">
        <location>
            <begin position="22"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="22"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="65"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="118"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG81773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="35052" checksum="1C8FCD1CC543C4CD" modified="2024-05-29" version="1">MSSWRGLCFLVWIMLIGQCQAQLKENFYSSTCPNVESIVNQAVSKKLRQTFTTIPATLRLFLHDCFVEGCDASILITSSDRDAERDAEENLSLPGDGFDTVIKAKEAVEAKCPGVVSCADILAIAARDVVRMAGGPSFNVELGRRDGLISKASRVAEKIPKPSFNLKQLYSIFSQLNLTQLDMIALSGGHTLGVSHCDQFSNRLHSFSSSAQTDPSLDPDYARQLTAACSDSDESTVIILDPETPQIFDNMYFKNLVAGKGLLSSDQVLFSDPASQPIVRDFANSPGEFNVAFVAGMRKLGRSGVKTGRQGELRKDCTAFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W557</accession>
    <name>A0AAF0W557_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acireductone dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Acireductone dioxygenase (Fe(2+)-requiring)</fullName>
            <shortName evidence="12">ARD'</shortName>
            <shortName evidence="12">Fe-ARD</shortName>
            <ecNumber evidence="12">1.13.11.54</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Acireductone dioxygenase (Ni(2+)-requiring)</fullName>
            <shortName evidence="12">ARD</shortName>
            <shortName evidence="12">Ni-ARD</shortName>
            <ecNumber evidence="12">1.13.11.53</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0102459</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:49016"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="12">Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the acireductone dioxygenase (ARD) family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.54" evidence="12"/>
    <dbReference type="EC" id="1.13.11.53" evidence="12"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W557"/>
    <dbReference type="KEGG" id="dcr:108226372"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010308">
        <property type="term" value="F:acireductone dioxygenase (Ni2+-requiring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010309">
        <property type="term" value="F:acireductone dioxygenase [iron(II)-requiring] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02232">
        <property type="entry name" value="cupin_ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000031">
        <property type="entry name" value="1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03154">
        <property type="entry name" value="Salvage_MtnD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004313">
        <property type="entry name" value="ARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027496">
        <property type="entry name" value="ARD_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418:SF0">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03079">
        <property type="entry name" value="ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0533">Nickel</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG83284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="200" mass="23510" checksum="0B8E367778F93D3A" modified="2024-05-29" version="1">MGSISKDEREEVIQAWYMDDSDEDQRLPHHRNPKEFVSLEKLAELGVLSWRLDADNYETDEKLKEIRESRGYSYTDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNDGWIRVWVKKGGMIVLPAGIYHRFTLDSNNYIKAMRLFVGDPVWTPFNRPHDHLPARKEYLESFVQKATANNSVDATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7U0</accession>
    <name>A0AAF0W7U0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0102964</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7U0"/>
    <dbReference type="KEGG" id="dcr:108205089"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="34"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="252"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="736"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="759"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="736"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="759"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG83786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1021" mass="115784" checksum="FDD05AC4F5C160EE" modified="2024-05-29" version="1">MVLLHSPSSSSEEGGDVQSKFKRLPIHSLKSKIVEKILENRVTLIIGETGCGKSSQVPQFLLEENMGPILCTQPRRFAVVAVARMVAAARGCEVGGEVGYHIGHSKVLSSSSKIVFKTAGVLLDEMREKGLKALKYKVIILDEVHERSVESDLVLVCVKQFLLKQNDMRVVLMSATADIARYREYFKDLGKDERVEVLAIPSSPQHTIHQQRVSYLEQVTELLEIRPESFSLKFDHHPLFAETVIKHEERVLIHDLVIHIHKNEPDMEKSILVFLPTYNDLEQLWFLLKRFPTAFKIHILHSSIDTEQALRAMKIWQSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVCWDNVRKKEVPDLVWVSKSQADQRKGRTGRTCDGHVYRLVTGSFYNLLEDYESPSILRMSLRQQVLSLCCAESKAINDPKVLLQKALDPPDPEVVKDALDLLVSIRALEKIPPRGRYEPTFYGRLLASFSLSFDASRIILKFGGIGLMRDGILLGILMDTQPLPILRPFGQNNLSTKYTASYYSGDSTETGLTGKKEEALVGNFAAYQFWQHVFKDKYRFEHLQDLLLSDETEKEKNLQSVTEENWCSFHHLVLSSLNHVAEIYDDVLNTLHRFRPNFLVKSGGLPSYYDPYEFQHACHLKCEHIELDGDVLDIYEDEHRDTVSKVQICTNEPFAVANSLPLDDVAVNLASIIKEIRSIQFVKEISSDQLNLNKYDSALESGEAYLCRYFLSGNCNRGDQCIFSHSLQAKKAACKFFFSLQGCRNGSSCYFSHDIEINHSSHNGLSLCLPEEEVTDFSTLLHLFPTSSDERILILDDTDFRFSSNIARFYNPSSIICTTSSAGGSVSDPSLMGIKILSNLSHPYQTLLNKEGESYIPWNQVKCVLWFPFSDGCRGDLEAEKRVVQTFFEYLAIRILADALFEVQVILTMNNIRFSQLQTEKLGRESFFLLKESFPYNESRFGVLSLSDTVPVKRPMLVSKAVSYVFCLHPPSSIEFDSSTPVLFQPLNFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W863</accession>
    <name>A0AAF0W863_DAUCS</name>
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            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0102643</name>
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    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
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        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF135">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN DAYSLEEPER-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
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        <location>
            <begin position="308"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="478"/>
            <end position="559"/>
        </location>
    </feature>
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        <location>
            <begin position="412"/>
            <end position="443"/>
        </location>
    </feature>
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        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
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        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG83468.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="66058" checksum="78B72DB04F15A4E2" modified="2024-05-29" version="1">MDKGKRKFEESNRITRYFSRADKQQANPASNNESGINVEGVYESMGRFVCSAEISFSSIKKLTMMMNPSFPFSYLELKRSCLKVYQEEKAKVVQTMEKLDGLIALSMDILRRDTYDYYSLELPEGRTVFDYLCLRAHFIDDNWEPKSWVICYIDTDHIFKNGAEKTILKCLSDFHIEKKISTITPRDRSEYDDMIETLKNLPNEKKKLQIKCQQFGIYCCSNLLCQMVEVAFEEIEDIIDRLSVRSSHWHITLSSLQEAIDLEAKGKFLEQYHSEHEDLPDEKEWKKLRYVCRLVAYIYNAAEVLFFTKNPTASLYLHNLHQLQASLRKESMSTDRLDLATDLLQKFDEYWNDMFLLLAIATVMDPRCKMKYIEFSFLKYDDNSGNSKVTTILEAIRGIYDDYKMHSTEALKSSKLSESKPSELKPPDRDSEEETLEEEEEDLPMHTLERLKNCNFGFNCMDEYNEFIKPGDQPPKSELEWYFDEPVLPWTKDFDLMSWWRTESPKYPVLSKMARDLLAIPFSVASSYEAFVYQDYRMADKSLDSLGPVLINALACTRSYSPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8M7</accession>
    <name>A0AAF0W8M7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">DCAR_0104513</name>
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    <organism evidence="5 6">
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        <lineage>
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            <taxon>Apiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF23">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="16"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG85325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="274" mass="30354" checksum="B961D4E7EEDC2131" modified="2024-05-29" version="1">MAYHQYRSPFGDTTFTKVFVGGLAWETPTHVLQEYFEQFGEILEAVIIADKNTGKSKGYGFVTFRDAESARRSCAEPNPVIDGRKANCNIASFGRPRPSPPRGRTQSGSSSSSTYSGVPGVALAPPVAPPPPVIYPPYGYVPYPSDYGYHQGMYNPQVQQQQQAQYYQQAYGVGVSSPSAISSPYYYGYPMQATRGTFYSGPQRIPPPQSYPYYHHLTQMQHQQQQEMFSFPGYPPPPRHSFPPPNPDPRTPQQTSTDQTEAGAEVVTSESPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WBE5</accession>
    <name>A0AAF0WBE5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104897</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <location>
            <begin position="1"/>
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</entry>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="492"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG88662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="738" mass="80744" checksum="F7F1B901188DCCE9" modified="2024-05-29" version="1">MDPNYSSGEEHLLHTKTRKPYTITKQRERWTEDEHNRFLEALKLYGRAWQRIEEHIGTKTAVQIRSHAQKFFTKLEKEALVRGPSTGQAIDIEIPPPRPKRKPSNPYPRKTSVGSLSLQAGTKDGNHLISDFSQSSAAHVLDLEKKQTLEKQSANQQLEIVKKNQNGVDCAEAFSFSQEALSGPLCSENKSTVSKTTSTNHCNFKEFVPNFKEVVNQDASHESYLTLKASGGRNDKEHDTKQLVGDLPPSETAKLGNYHPTHDKLLQTQKVNELKPPVNYGILSSYDMQAAAHYPRHVPVHIVNGSLEIAQTGASLYGNVAPALTTEHENNASRSSVHHFPPFHSPFTPVQNPQSDCHSLLPIPATFTSLVASALLENPAAHAAASFAARFWPVANVDPSADPHVGKMENTGPSMEALAAATVSAATAWWASQGLLPLCTSFNATCSAAPTITTTMENNEARVSNDKSGENPADLSLKDQQLEPKCSETLLDHQTAVKSPPLSSSESAEGESEKLNIKLSNVDPEQAEAVTKAQDLSKTKDQKLVDRSSCGSNTASSSEVETDALEKHDKEEANEPDLSLQISDSTNRRGRICSNLNESWKEVSEGGRLAFQALFSREVLPQSFASPYCHQKYSIGEDKQHLYKEENERLQIDINSKACGSNEFVEHNVSLTGGSNEGEGTLSTGLGCLKFKARRTGFKPYKRCSVEAKESRVAAASTINLEEGKAPKKMRLEGGASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM07</accession>
    <name>A0AAF0WM07_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0311340</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM07"/>
    <dbReference type="KEGG" id="dcr:108213266"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000244">
        <property type="entry name" value="Serine/threonine-protein kinase CDL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF39">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="83"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG92082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="386" mass="43243" checksum="2BE096A3742AF349" modified="2024-05-29" version="1">MSCFSCCLSEDKVNRRSLRKSIQEGRDARTIASSFASSFANLSFKSDGSRRRYIVGEISKYGKGSVTTEFFPFKELSAATQNFRPDCMVGEGGFGRVYKGHLESKNMDVAIKQLDRNGFQGNREFLVEVLMLSLLHHPNLVNLVGYCTDAEHRILVYEYMANGSLEDHLLDIGEKKPLDWKTRMQIAKGAAKGLEYLHDTADPPVIYRDFKASNILLDENFNPKLSDFGLAKLGPTGDKTHVTTRVMGTYGYCAPEYALTGQLTAKSDVYSFGVVLLELITGRRVIDKSRPSEEQNLVTWAQPYFRDKRKFHLMVDPLLENQYPKKGLHQALAVAAMCLQDEDETRPYINDVVSALEFLCDSDSKEGVMSAASSMAEGRSMAEGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPV1</accession>
    <name>A0AAF0WPV1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0313147</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPV1"/>
    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG93860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="30193" checksum="D79699AC9E36B25E" modified="2024-05-29" version="1">MDKILDTFITEHEEAAGTTRPKGYEPDFVDILLSVFDKHEENRGDSIDVIDRDSVKAILVDMIVAGIDTSRTTIEWIMTELLRHPRILKKLQQEIKIVVGDADIVEDKHLSKLEYLDMVIKEDFRLHSTVPLLIPRESMEDIVMDGYFIPKKSRILINCWAIGRDPAVWSENVLEFIPERFADKKIELKGHDYELLAFGSGRRVCPGMNLGLLKVRQIVAQLVHSFDFELPNKMLASELDMDEKFGLALPRDNHLCLLPTVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRV2</accession>
    <name>A0AAF0WRV2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0312621</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF81">
        <property type="entry name" value="OS06G0145700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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            <begin position="1"/>
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        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG93338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="383" mass="43022" checksum="9F082526C870B60D" modified="2024-05-29" version="1">MATMFIKREEKPSRRRLSMADGEGQDVKPVKRRRRDSAAIARGSDAPQQPQPQQADQASAPAPTTVKRSSRFRGVSRHRWTGRYEAHLWDKLSWNVTQKKKGKQGAYDEEESAARAYDLAALKYWGTTTFTNFPVFEYASELQIMQGVTKEEYLASLRRKSSGFSRGVSKYRGARIGRVFGNKYLYLGTYSTQEEAARAYDIAAIEYRGINAVTNFDLSTYIRWLKPGATSPGIPQVPEINVEPQSVTYDNFNTNKEGHSSFYNAKAFAVDDMKTEQQQEALPRSHSLTSFNKKSSSPTALSLLLKSSIFKELVEKNSLINKEENNVVDETANQPQISSDDEFGEVVYDGINGDIPFSLSSTNDHGFEFQGQFSFSSLLGTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT83</accession>
    <name>A0AAF0WT83_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Apiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="193" mass="22798" checksum="BC4FB1DDE4CB1C4E" modified="2024-05-29" version="1">MIDLLVKNGPKRGHSLIKGPKDNFSRLFTTNLYTRVLSNIEKCDRDWFVYSKELDRIFYFCCKIFKKGIGRGQLTNEGFSNWGHVGLRLKEHETSIDHVKNMITCYELRLRFQKNETLDKTTQTLIEKEKYHRKKVLHRVISIIKYLAKHNLAFLSNFLGLVQMLAEYDPIIQEHVRRVTSTEINSHYLGHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WTJ5</accession>
    <name>A0AAF0WTJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Derlin</fullName>
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    </gene>
    <organism evidence="9 10">
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in the degradation process of specific misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Endoplasmic reticulum membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WTJ5"/>
    <dbReference type="KEGG" id="dcr:108213051"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG94313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="240" mass="26955" checksum="42C9C451A536411E" modified="2024-05-29" version="1">MSSPGEWYNSLPPISKAYGTLCLATTIAVQIGVLAPGFIALAYEYVFYRFQVWRLFTNFFFLGGFSINFGIRLLMLARYGVQLENGPFQRRTADFLWMMIFGALTLLALSAIPGFQSYFLGISLVFMLLYVWSREFPTGNINIYGLVTLKAFYLPWAMLAMDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKTMLQTPMWVHKIVARWRIGAPPTTRAEPDRSGSTAFRGRSYRVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WV16</accession>
    <name>A0AAF0WV16_DAUCS</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0415354</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WV16"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG96024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="251" mass="28190" checksum="21627B56FAAFD1BC" modified="2024-05-29" version="1">MLLKTLDLKTEKISLLVQMSGTQPTFPNKLYPIDLPVGVLLKSGTTVVREYKENVVIKIIDNSVNYLLFLYHCQRFGSVLERNEASRVFASEEAEAEARKESVKTFVPGEIPAPEVSKEEQPPKKVAPTPEQIIAIKAAILNSQTLEEVARLEQALKSGQLPADLEIIDTDPVTVDTNTNVDKMITESEKEAKDGTSDVNEQKMMGLQTWSRYNWCNIVRLIHSTKNIIDGCSFRPGQFLTQLKIQGKRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WYQ5</accession>
    <name>A0AAF0WYQ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0416711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WYQ5"/>
    <dbReference type="KEGG" id="dcr:108216414"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF1">
        <property type="entry name" value="PROTEIN SCO1 HOMOLOG 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG97371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30093" checksum="EE28D510C4934E43" modified="2024-05-29" version="1">MFSSRLLFSSLRNRSAVISNSLARFDCSKRFHSSRYLRSVRQTNQHLMGTTTQRTQSGSWTSYVIPPIILGGIGGVAYLIHYNDERRVILKGDAVNCGINSTKGPVIGGPFNLIDTEGRSISEHNLLGNWVLLYFGYTSSPDVGPAEVEKMSKAVNILESKQDLKVLPVFVTIDPQRDNPSQLRAYLKEFDSRIVGLTGSVAAIKDMAREYRVYYKKVEEDGDDYLVDSSHNMYLMNPKLKVLRCFGLEYSADELSESILKEMKSDGI</sequence>
</entry>
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            <fullName evidence="10">LMBR1-like membrane protein</fullName>
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        <name evidence="8" type="ORF">DCAR_0416741</name>
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    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG97401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="dcr:108219273"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LMBR1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
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    </feature>
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            <begin position="481"/>
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            <begin position="705"/>
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            <begin position="565"/>
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        </location>
    </feature>
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            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WZL3_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0418381</name>
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    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Scandiceae</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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    </dbReference>
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    </dbReference>
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            <end position="79"/>
        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG99035.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="195" mass="22249" checksum="E32D2197F7ED38AF" modified="2024-05-29" version="1">MKAVDDYYVQVVADTCKNSSAASLVVEGESSSSSVSRSFDHNRIKCNSWKTRYKFWAPLTIILLLALCSMFTGSVTLHWSTAAAALPDDLDYPLHSDLDILEVEERVKMVRRMWNVYTHSNTIKLPRFWQRAFQAAYEDLTSHVSSLRNNALLEIAQMSMFSLQSLPQPYSSIYPRHSTLGGSRNLVRRVSFLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZP2</accession>
    <name>A0AAF0WZP2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="2">Nuclease associated modular domain-containing protein</fullName>
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    <organism evidence="3 4">
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOG99080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003611">
        <property type="entry name" value="NUMOD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199:SF2">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07460">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Nuclease associated modular" evidence="2">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
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        </location>
    </feature>
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        <location>
            <begin position="539"/>
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        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
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        <location>
            <begin position="323"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="598" mass="68420" checksum="2AEF6A1DAD27A57E" modified="2024-05-29" version="1">MLAKKLNSSQHSFHYHLLPFGDHKLCHLNDTRLSYLSRSFKIPAQKRKFSVGHIKMQPREVLVSAVATVEPKISFKSDSRDDQRLHIGTCSERSQGVEHQSSSEEFTDVDDREKLRRMRISKANKGNTPWNKGRKHTPETLRKIREKTRLAMQNPKVKMKLVNLGHAQSKETRIKIGVGVRMGWNKRREKLMLQETCHFDWQSLIAEASRRGLNGEEELQWDSYKILDEQLEREWLESVEERKRSPRPKGSKRAPKSADQRRKISEAIAAKWADPGYRDRVYTGLSKYHGTPIGADNPRRRHKRTSSNTNTSKKKKNVADISARNTTKSTTQKAIKKRSKMPIYTDPLASSKLEMIKNIRADRENKKAEAITRAKLLIAEAEKAAKALEIAATKSPLAQASLIETRKLIAEAIQSIESIEGLFDENNLNILSSQTVSNVEREVDMKSDGLFTKNQIEINGSRTLISNFDDISDSTFEKFNLQELLNHEDELLPSSSYDVEIMNDKEYLKELLNTGINLSQTKPLQKKVEPSSFTHQLDHLTPNGSSIKHENLVRNGAESELWEKEKPPKSTREGNIPKSINSTKKWVRGKLVEMTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0F2</accession>
    <name>A0AAF0X0F2_DAUCS</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0417539</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108217447"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044610">
        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF4">
        <property type="entry name" value="CORE-2_I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG98198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="411" mass="47816" checksum="D335DBD7419D420E" modified="2024-05-29" version="1">MVSKMISSKFQVTSFRGYLISFFLTSLIFSLFYMSAFFKTFNNVTTLWSSKPLVIPEPSYPVRFAYLISASKGDVVKLKRTVLALYHPGNYYLIHMDSEASEEEHSEIAEFVPSYDVFEQMENVWVVDKPNLVTYRGPTMLSATLHAMSILLRKASEWDWFINLSASDYPLVTQDDLILAFSDLPRDLNFVQHSSNLGWKVNKRANPVMIDPGLYSLNKSEIMWADKQRPVPTAFKLYTGSAWTVLSRSFAEYCIVGWENLPRTLLMYYTNFVSSPEGYFQTVICNSEEYRNTTVNHDLHYIAWDYPPKQHPRLLGFRDYRKMILSHRPFARKFKQDHGVLDRIDRDLLSRQNGEFTTGGWCYKRDNSTESCSGLQREKFGLLRPGTGARRLRSLLRKTLYTDDFETRQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0Y3</accession>
    <name>A0AAF0X0Y3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PORR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0518702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PORR_domain"/>
    </dbReference>
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        <property type="entry name" value="PORR_fam"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="20"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="403" mass="47420" checksum="9A79E9F3EEE917E5" modified="2024-05-29" version="1">MSRALYYLRFSRNMSLWSMKKDPDLESALSRNRRWIVNNQIKNIILRCPNQVASVKLLQKKFKTLDLQGKALNWLKKYPCCFDVYLENDELYCQLTKRMMFLVEEEESVKDMQEGVYVERLAKLLMLSVNQRLSVLKLNVLRRNFGLPDDYIIRIIPKFPELFRVVNYSGRRSSMEIELLSWNPKLAISAIETSSERQGMVPSYSCSLPSSWVKSWERFHEFNSTPYISPYSDTGGLVEGSKEMDKRTVGLVHEILSLTLWKKISIIKLGHFKREFCLPEKLNGLLLKHPGIFYVSNRYQIYTVLLRNEYKGSELIDKDPLVVVKDKFGELMQEGLHEYNRRHYFQNLEKNRKKGIIPVKPEKRIEKISEMTEQNDQGGDLGGILDPEERKRFYRVLFDDTCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X133</accession>
    <name>A0AAF0X133_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0418020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X133"/>
    <dbReference type="KEGG" id="dcr:108216730"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG98676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="126" mass="13777" checksum="2712E73C80925209" modified="2024-05-29" version="1">MGILDQLRWVAGRAIAVSSVVDHNLKPVSGGSQVYKGVKDEALKAGTFQSSYKKKSHGSRGLAIMVEEMQAKVETVEEERNSATQRQSSTNCVKASDNLGELLAEPSDESTPQKANRKKVFIRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3Y4</accession>
    <name>A0AAF0X3Y4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    <dbReference type="KEGG" id="dcr:108222016"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF417">
        <property type="entry name" value="LACCASE-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041774297" description="Laccase" evidence="13">
        <location>
            <begin position="31"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="44"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="170"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="433"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH02724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="581" mass="64012" checksum="FFCB86412683A73F" modified="2024-05-29" version="1">MGVSLTLPGAILRFSLSIMCFLLHLASATASNAGAITRHYEFNIKLQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVINHVPNNISIHWHGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRGTLFWHAHISWLRSTLYGPIIILPKLNVPYPFPKPHKEVPIIFGEWFNADTEAIITQALQTGGGPNVSDAYTFNGLPGPLYNCSSKDTFKLKVKPGKTYLLRFINAALNDELFFGIANHSLTVVEVDAIYVKPFETETILLTPGQTTNVLLKTKPNYPEASFLMTARPYVTGSGTFDNSTVAGVLEYKGSSHHSTKKLPLFKPTLPSLNDTSFAANFSKKLRSLATAQFPANVPKKVDKQFFFTVGLGTSPCGLKNQTCQGPNGTRFAASINNISFIQPTTSLLQSHFLGQSDGVYKPDFPVSPLQWFNYTGNPPNNTLVSNGTKLMVLPFNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGDYNPNVDPRSFNLVDPVERNTVGVPSGGWVAIRFLADNPGVWFMHCHLEVHTSWGLKMAWLVLDGKLSNQKLLPPPADLPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAX6</accession>
    <name>A0AAF0XAX6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0623132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108227453"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049152">
        <property type="entry name" value="EFR3-like_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055296">
        <property type="entry name" value="SRL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46087:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46087">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21052">
        <property type="entry name" value="EFR3_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="995" mass="110665" checksum="3A69E640A9122078" modified="2024-05-29" version="1">MGVMSRRVVPVCSNVCFFCPSLRARSRQPVKRYKKLLADIFPRSQDAEPNDRKIGKLCEYALKNPLRTPKITEYLEQRFYKDLRNEHFGSVKVVLHIYRKLLSTCKEQMPLFASSSLGTVRTLLEHTGEDEMQILGCNIIVEFVNNQVDSTYMFNLEGLIPKLCQLAQEVGDDERTLQLRSAGLQALAFMVKFMGNHSHISMDFDKIISVTLENYMDLSMNPETTKQDGHSTQSQEQWVQEVLKVDNNGSSFPNIGMKISSTDNLINTKRGVADMMDTHGTPSYWSRICLHNIAQLAKEATTVRRVLEPLFNSFDNEDLWSSDKGIACSMLTYLQTQLEESGGNSHLFLSLLVKHLEHKNVGKQYSMQIDIVNVATQIAENVKPQSSVAIIGALADLMKHLRKCIQNAIESPNPEDGSYKCNLDLQIALENCISKLSHKVGDVGPILDMMAVVLENIPVTGVLARTAVYVVYRTAQIISSLPNVLYYKKAFPDALFHHLLLAMAHPDHETRIGAHHVFSTVLLPSLTTPWSVHGGYPSQDLLGPLLVGSIERHSFQNASKTNSGRMIAPMEASGVVGMHLDKPKEKPSFGQTYSFKHSSTDISNESTSLRLSSHQVSLLLSSIWVQATTFDNTPTNFEAMAHTYHLVLLFTLSKHSSHKALARCFQLAFSLRSVSLDHEGGMQPSRRRSLFNLALYMLIVTARAGKFSKLISPVKSSLTSETADPFLELVEDNKLKVARSQLGDARNSYGSPEDEIAALKSLSAVKSGDEVLKEMVVEHFISCKELSEEELSSIKKQLLEGFSPDDAYPLGGPLFMETPHPCSPLARMEFQAFDEAMPSALMDEEAFPDLYGSSSGRKTALPVKSIDILSVNQLLESVLETARHVASFPISSMPIPYDQVKNQCEALVTGKQQKMSALQSFKSQHEAKVVLSLNDSEKETPLANGVLKVPELDVTVATLEQPSQLVVFPHEHVQQQSFRLPPSSPYDKFLKAAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XBF4</accession>
    <name>A0AAF0XBF4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XBF4"/>
    <dbReference type="KEGG" id="dcr:108227078"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="209" mass="23562" checksum="00C234754B8AAEE7" modified="2024-05-29" version="1">MGVGMSRLNVASCGAFHRTTSELQDPSTYTRMMVDRNDHDNSKIIPVQGQKNNSSLPKEVVSSSLPKKDVVSSKKDDEEKNTYVNNIYQQLDEESEKNTDYDGPMFMKSPSFRDFMRPVEGSVKNGGKKKGEMKETNKTEAQYDYICNGEACVRVESKNDDTPPVMNKEAGPRKDKKINKIFAQYYKPSAGVKNLFNPHPKHPKPSNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    </reference>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH04142.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="282" mass="31637" checksum="188C3AE0EF11F32F" modified="2024-05-29" version="1">MNCNNSLKYKDHDQLITITRNSLKKATKLLLSVSVFSLLLSYSSSLYPIFIHSFKSNSLFPHNFLVLFTHILDKNFTFLICNGILVFLAKTSGLLRSRSGIDHRRRIKSIDTVSIQMHHPATGTSLEELENYHNQEVADVAEYSYVSPDSSLKDVPAAAGWGSSSTNHDEDLFSEEGKNVKGGERGEAKEGEVEETGFLSTELGRGAEEDHTDYPFTNDENTEIDLGGSFFSFKKEDADENCQLESSGLVSTEELNKKFDEFIRKMKERLATEEAGQKLIMV</sequence>
</entry>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108224443"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APRL5/7"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        </location>
    </feature>
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        <location>
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            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="72"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH07195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="33822" checksum="54AD72223A1133A2" modified="2024-05-29" version="1">MSKSTATSTALLLVCFSTVFSAASPPPTCPLQPNLFIHTLNSQSPFHFSPNPPLQVGSDYLDRILNSRGNNAYTSVLFYASWCPFSHKVRSTFKTLSAMFPHIEHLEVEESSVVPSILSRYRVHSFPTILMVKQTSKLRYLGAKDLNSLVRFYRRTTGWEPIQYVDTEQASSSEGSGEFVMRSWVGSSPYEILRREPYLVLSLLFLCLTVLINALPKVLLHIKPLWVVYGPHLNLGIFGETRQILGRLLQMVNLKMIWTKLKRCKTSGFHQCAKDVKNARVWASSLASVSLGKTSSSKSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJG9</accession>
    <name>A0AAF0XJG9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0728356</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:phosphorelay sensor kinase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:cellular response to abscisic acid stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:phloem or xylem histogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of flower development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of seed germination"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histidine kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000015">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.350:FF:000001">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="entry name" value="CHASE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056839">
        <property type="entry name" value="Receiver_AHK4/CRE1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF73">
        <property type="entry name" value="HISTIDINE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24896">
        <property type="entry name" value="Receiver_CRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="14">
        <location>
            <begin position="154"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="454"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="895"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH08905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1033" mass="116059" checksum="4FEB34019609E930" modified="2024-05-29" version="1">MSYFYVVGFALKVGQLLLMLWGWIISLISMSWFINGGIMSTKNDLLGDGRKLWLVWWDKISAYKIYNFYFEYFSPKRFRKSWWRNLLVTWVLIGTLFSMWVFWYMNSQALEKRKETLASMCDERARMLQDQFNVSMNHVQAMSILISTFHHAKNPSAIDQGTFARYTERTAFERPLTSGVAYAVRVLHSEREHFEKQQGWKIKRMDHSDQVPVPKGVYDGEESEAPEPSLIQPNQEEYAPVIFAQETVAHVISIDMLSGKEDRDNILRARESGKGVLTAPFKLLKSNRLGVILTFAVYKIDFPSNATPDERVQATDGYLGGIFDIESLVEKLLQQLASKQTILVNVYDTTNLSSPISMYGSNEFEDLLEHVSPLNFGDPFRKHEMRCRFKQKRPWPWLAITTSYGILVITLLVAQIFHATMNRITKVEDDFQKMRELKKRAEAADVAKSQFLATVSHEIRTPMNGVLGMLHMLMDTDLDVTQQDYVRTAQASGKALVSLINEVLDQAKIEAGKLELEAVRFDLREILDDVLSLFSGKSQDKGVELSVYISDKVPEMLIGDPGRFRQIITNLMGNSIKFTEKGHIFVTVHLVDEVMDMTEVEKESSWQNTLSGLPVADQSKSWAGFKILNPEGSNSSVSSSSDTINLIVSVEDTGVGIPLEAQSRVFTPFMQVGPSISRTHGGTGIGLSISKCLVGLMNGEIGFVSVPKVGSTFTFTAVFTNGGNSSYSQKIQQINQQSGYKEFQGMKAIVVDSREVRAKASRYHIQRLGIQVEVVIGLSHGLSVMSSGKKVIDMVLVEQEVWDKDLGMSALFVSRLMNFDQGVPPKVFLLANSVNSSRNGHATLGNYYPYVIVKPLRASMLAASIQRATGAKNRGNYRNGELPSLSLRSLLLGRKILVVDDNNVNLRVAAGALKKYGADVVCADSGKKAISLLEPPHQFDACFMDIQMPEMDGFEATRRIRDMESKINGRIQHGEMLKEAHKDISRCHIPIMAMTADVIHATNEECLKSGMDGYVSKPFEAEQLYREVSRFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XK85</accession>
    <name>A0AAF0XK85_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0727975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XK85"/>
    <dbReference type="KEGG" id="dcr:108196360"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000001">
        <property type="entry name" value="Magnesium transporter MRS2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF31">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH08534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="389" mass="43427" checksum="F264DAC27DB788F6" modified="2024-05-29" version="1">MGRDVVPVDPSQPAVGLKKTKARGWIRLDSSGQDTILDVDKYAIMHRVHIHARDLRILDPLLSYPSTILGRENAIVVNLEHIKAIITAEEVLLRDPSDDNVIPVVEELRRRLPLLNNTREDHGEGKELGGQNDGEAGEEDESPFEFRALEVALEAICSFLAARTTELETSAYPALDELTSKISSRNLDRVRKLKSGMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASPLSGSAAASWFISSPNISSKISRASRASMATIRGDENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVAAIFGMNIPYTWNDNHGYMFKWVVSLSGIASAVLFLLIISYARLKGLVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKG2</accession>
    <name>A0AAF0XKG2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase Pif1-like 2B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0728116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 (TC 1.A.33) family. HSP110/SSE subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH10756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108196185"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ASKHA_NBD_HSP70_AtHsp70-14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="840"/>
        </location>
    </feature>
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        <location>
            <begin position="787"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00061090"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH10756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="840" mass="92819" checksum="4176B7D27126FF1F" modified="2024-05-29" version="1">MSVVGFDFGNESGVVAVARQRGIDVVLNDESKRETPAIVCFGEKQRFLGTAGAASSMMNPKNTISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLINAQYLGEMRSFTPTQVLGMVFSDLKSIAEKNLNAAVVDCCIGIPIYFTDLQRRAVLDAATIAGLHPLRLIHETTATALAYGIYKTDLPENDQLNVAFVDIGHASMQVCIAGLKKGQLKILAHAFDRSLGGRDFDEVLFQNFAEKFKTEYKIDVYQNARASLRLRAACEKLKKVLSANPEAPLNIECLMDEKDVRGFIKRDEFEQISIPILERVKKPLEKALSEAGLTVENIYAVEVVGSGSRVPAIMKILTEFFGKEPRRTMNASECVAKGCALECAILSPTFKVREFQVNESFPFTIALSWKGTAPDAQNGADNQQSTIVFPKGNSIPSVKALTFFRFGTFTVDVHYADVNEVQAPAMISTYTIGPFQSTKSERAKVKVKVRLNLHGVVSIESATLLEEDVEIPVVKETAKMDADESPPVPAPSSGAENDVDMEDAKSTAANAPGSANGAQGDVPVQMETESKVEVPKKKVKKTDIPVTELVYGGMQPTDVQKAVEKEFEMALQDRVMEETKDKKNAVEAYVYDMRNKLHDKYHDFVTDPEREEFTAILQQVEDWLYEDGEDETKGVYVAKLEELKKRGDPVELRYKEHLERGSVVDQLVYCINSYREAVMSPDSKFDHIDIAEKEKVLKECADAEAWLREKKQHQDSLPKHATPVLLSADLKKKAEALDRFCRPIMTKPKPKAAKPVTPEVSSPSSEQFHGAENSGDPNKDASNTPGTEVPLASEEPMETETPASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XU30</accession>
    <name>A0AAF0XU30_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oberon-like PHD finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0832328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XU30"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
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        <property type="entry name" value="ADAPTER PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33345:SF6">
        <property type="entry name" value="OS03G0747200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="6">
        <location>
            <begin position="4"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH12819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="73" mass="8147" checksum="58DA97417C7563BC" modified="2024-05-29" version="1">MAGTIGAEYYCRCCDTRTDLLPHVKIFLQICESISSHDDIKKILNLGVYVLQGSQRSAAERLLLVFEIAMGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XU84</accession>
    <name>A0AAF0XU84_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0832403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XU84"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053031">
        <property type="entry name" value="Cuticle_assoc_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396">
        <property type="entry name" value="OS03G0264950 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396:SF27">
        <property type="entry name" value="OS08G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="WOH15909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM repeats" evidence="4">
        <location>
            <begin position="349"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM-like" evidence="3">
        <location>
            <begin position="512"/>
            <end position="865"/>
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            <begin position="929"/>
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    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="1171" mass="131467" checksum="D2DB8A477990AFBA" modified="2024-05-29" version="1">MIVFKDFAPKLCTNIFESCSVSGVNARTKSGAMNHVSCEGEIGEGLRLVAKQYKGWLMYYQVMFYGDASRSRRVSATFPDNYNKSENSLNMKSSSESSHPPERECSQQAYSNFEKVHPLDTLEKVPIASAEESKVSTYLRSLLVDDRSRTSDIRCLRDILKESQSSTPVSSSSRTDFIDDDDLQEYAEASEASFGLERTVSGNPEEVSDQFKSVILNFEHACIYLSRNRQSPSSSIHRWDAVIRREDMTIFGSGTFSSSLRNLNLSQLEAGLFESHTFLNSHMEEETSRRRMHLQDFEQTDQIGSATLQNYYYSQEYPPGNSARRKKKFSSENSIDEIFLHPEENSHIEQVGVLEKLISKLCFSENLGEEDYTVEITTVYEILSKKTGAKYSMLKDIIIDQLLMAISTSKEEGVIRTSVSILSTIISVNKSVVEDIKKKGLRLSDLVTALKRNVYEAATLIYFVNPSPAEINNLELLPVLLEVICSSNSQKCSLSSPKLTPPAASLMIIEVLITACDYATNNMHLEAISSPRVLCGLLNAPKQNNLQEFISLAAVLVRCMRFDGKCRKQISQFTPIAPVISLLLSEHKPAIYAGLEFFNEILRIPRTSAISLLQLVEKEGRTNDIRKSLLQILQSQPEHKLLAANLLLHFEMLEDLSGTSIYCTEAMEIILETMACDETPATQKNSAFILSNLGGTYAWTGEPYTVAWLVKKAGLSSLHHKNIIRKIDWSDQSIQDAGTDTWSSKIARHIIKFGKPVFHALVKGLQGNSKRIARECLITLAWLGCEIVKTPNDLRYSACEILLDSIEQYVHPGLELEDRLLACLCIYNYASGRAMQKLVHFSEGVRESLRRLSSITWMAEELLKVADYLQPDKWRISCVHTQVLEMGNNSSGSVNALIYFRGELYSGYADGSIKAWDIKGQSATLVRDIKAHKKAVTCFSLFEPQNCLLTGSADKTIRMWQIIEQKLECIQVIAAKSSVQSLDTSGNMIFVVTQGNNMKVFDTSKKDTDVYKKKNVKCINAKEGKYYLGCLDSSLQEVTLINNRHQELKAPIWSWRIQRKSINSIALYKDWLYSASSVVEGSSVKEWRRNSKPQMLIVPGKSTTITAMRVVEDFIYLQCNSSANSLQIWLRGTQHKVGRLSAGSKITSLLTANDMVLCGTETGLIKGWIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIA8</accession>
    <name>A0AAF1AIA8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="17">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0102724</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. Plant myosin class VIII subfamily.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01383">
        <property type="entry name" value="MYSc_Myo8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Unconventional myosin-XIX"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
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        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
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    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="MYO1-3_N_SH3"/>
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        <property type="entry name" value="Myosin_head_motor_dom-like"/>
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        <property type="entry name" value="MYSc_Myo8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF780">
        <property type="entry name" value="MYOSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25369">
        <property type="entry name" value="SH3_VIII-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 10" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="14">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="165"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="10">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="978"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="256"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG83548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1147" mass="129769" checksum="5115C5D80C5AA9EA" modified="2024-05-29" version="1">MSHKVERAPHLQSIRSLPVDFRLKDALEDHSSRNSETVTMMNSTRVYNSLTKNVEVVGTNINNKVDSNGFDESPYGKGPYRGESLNSAASTSQSYGSSFNDSKWSDTSYDAKKKLQSWFQLPDGNWELAKILTTSGTELVISLSDEKVLKVSGDTLTPANPDILDGVDDLMQLSYLNEPSVLHNLQFRYDRDMIYTKAGPVLVAINPFKKVPLYGNDFIEGYRRKTIASPHVYAITDTALREMIRDEVNQSIVISGESGAGKTETAKIAMQYLAALGGGSGIEYEILKTNPILEAFGNAKTSRNNNSSRFGKLIEINFSETGRISGANIQTFLLEKSRVVQCAEGERSYHSFYQLCAGAPPSLREKLNLKTAMEYNYLRQSSCYSIPGVDDAKEFRVVTEALDVVHVSKEDQESVFAMLSAVLWLGNVSFAAVDDENHVEPVNDEGLVTVAKLIGCNVADLKLALSTRNMKVRNENIVQRLTLSQAIDARDALAKSIYSCLFDWLVEQINKSLVVGKRRTGRSISILDIYGFESFIRNSFEQFCINYANERLQQHFNRHLFKLEQEEYIQDGIEWARVDFEDNQDCLSLFEKKPLGLLSLLDEESTFPNGTDLTFATKLKEHLHSDPCFRGEREKAFTVCHYAGEVMYDTTGFLEKNRDLLHLDSIQLLSSCQSNLPQAFAANLVAQSESSLSGLLNKSGGADFQKLSVATKFKGQLFQLMKRLENTTPHFIRCIKPNNNQSSGLYDQGLVLQQLKCCGVLEVVRISRSGFPTRMSHKKFARRYGFLLLESVASQDPLSVSVSILHQFGILPEMYHIGYTKLFFRMGQISVLEDTRNRTLHSILRVQSCFRGHQARLMVKDQRRRITAFQSFIRAEKTRKEYSVLLKQYRAAVAIQKQFKGRIGREKFKHIQDASVLLQSVIRGWLVRRCSGDAALLQFGVMKGHETDNVLVKASYLADLQRRVLQAESALRVKDGENDILQQRIQQYDNRWSEYERKMKSMEEMWQKQMRSLQSSLSIAKRSLAADDSDASVNNDSSYGSPVDYTRPTNTGLSVISRLAQEFDQRSRIFGDDAKFLVEVKSGQVDANLDPEQELRRLKQMFEGWKKDYGSRLRETKVILNKLASDGGSGEKMKKKWWGRLNSTRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AM49</accession>
    <name>A0AAF1AM49_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0207509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AM49"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33728">
        <property type="entry name" value="CTTNBP 2 AMINO-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33728:SF3">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="147" mass="16505" checksum="ACD57B8D0CD4C83A" modified="2024-05-29" version="1">MSEFDSERAKPWNIYTGSQPNPSQAGVDRQAPWRNFGTSMNAISFGFVATAILISMFLIMAIFEHLFRPNASFTSLEDGVNRPATESRRMQKLGHQPMVPTTYASDFSVVMPGQRCPTYIAQPAPLPCKREGASWPSHEHTYSLHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AP68</accession>
    <name>A0AAF1AP68_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-glucosidase 11-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0206524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AP68"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000020">
        <property type="entry name" value="Beta-glucosidase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF29">
        <property type="entry name" value="BETA-GLUCOSIDASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042220211" description="Beta-glucosidase 11-like" evidence="5">
        <location>
            <begin position="29"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG87301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56303" checksum="2D5C9E35EDE422AF" modified="2024-05-29" version="1">MPNLGLPRGQLLIFLLQLTSLSVLQVYGIGINYSRDDFPADFVFGSGTSAYQVEGAPFEDGKTPSTWDIFTHDGYVNNGETGDIACDGYHKYKEDVKLMADTGLEAYRFSISWTRLIPNGRGPINPKGLQFYNNLINELITWGIQPHVTLFHGDLPQSLEDEYGGWLDKKILKDFVVYADACFREFGDRVMHWTTFNEINMLPMLGYDAGMMPPRRCSSFYGMNCTQGALDEPYRVAHNTLLAHASAAKLYKQKYKPVQHGFIGLNVLAFWFSPYTITTEDLLATQRANDFYVGWFVNPLVKGDYPDVIKKNAGKKIPSFTKTESLRLKGSFDFLGVNHYASGYVKDQTINPAVETRDLMADMAGLTHVLEYFKDVYGNPPVYIHENGLRTVRNGSMSEELNDAARVKYMRGFIGSVLDALRNGANTKGYFTWSFLDVFELLGGYDSAYGLHYVDLNDKDLKRYPKSSATWYSSFLKKRGIQSVGQGKDSVSELFHSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1APF9</accession>
    <name>A0AAF1APF9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0309903</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1APF9"/>
    <dbReference type="KEGG" id="dcr:108210434"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
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    </dbReference>
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        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="zf-C2H2"/>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="31"/>
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        </location>
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            <begin position="1"/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <begin position="126"/>
            <end position="181"/>
        </location>
    </feature>
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        </source>
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            <dbReference type="EMBL" id="WOG92459.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="181" mass="20681" checksum="B11C238246A4FCB1" modified="2024-05-29" version="1">MFHARCCAHILNLCVQDGLDPTEEIVDKVRDGVKYIAASEGRRIKFAEISKSLGLKCKKLILDVSTCWNSTYNMLACAIEFKNVFLIYITSDQGFKVYVPSEEDWERVAGVCFFLEVFSDVTKVVSGTGYSTSNLFLSKIRRVKKIIDKKAIDPNLHIREMARKMELKFEKYLGETNLVMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AT06</accession>
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    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MICU1 family. MICU1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG91321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108211254"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh_MICU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG91321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="482" mass="54939" checksum="996D60978DAD60B3" modified="2024-05-29" version="1">MHSSWAVLRKPRSSIIFTFSRKTYRPFFNQANKNSSAPLVSLSNLCGGGGWNNRYKYSGNDKSNDVKCSYFSWFLRSVCLGVVVGSSSNSCIAYADDSQQQYADAAVKKPMFLFGDSYRKKVFFKYEKRIRMQSPPEKVFEYFASYRAPDGEVFMTPADLMRAVVPVFPPTGDTRVRVGSLKGEWSPGDLQCAPSEFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDLDNNGEIDKDEFRKVMNIMRSQNRQASRHRDGKRQGLKVSGSVEEGGLLEYFFGKDGKACLEHGRFVKFLEDLHEEILQLEFKHYDHKSRKAISAKDFALSMVASADMKHIDKFLKIVDQLEDEPRMRDIHITFEEFKNYAELRKRLRPLSMAISSFGKANELLTKADFQRAADQVCGIQLSNNVVDVIFHVFDVNHDGSLSAEEFLRVMQRREGDGTLPREAGVMGLISCWLSCTKSCKSAQIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU28</accession>
    <name>A0AAF1AU28_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0311558</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:sugar transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF30">
        <property type="entry name" value="SUGAR TRANSPORTER SWEET1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG92295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25293" checksum="C64891F0FDC069F9" modified="2024-05-29" version="1">MANEIILNAFGILGIISSFGIYACPMLTMHKILLKRSVQQFSPDPYLGTAINCMFWILYALPVVHRGSILVLIGNSIGLGLECVYLIMFLLYAKSNQQRIYIISIFIVELAVIGLVGGLVIGLVHTDEERSRIIGILCIIFNLAMSGSPLTIAKQVIQTKSVEYMPFFLSLTNTLSGVFWLTYATIRLDLNILIPNIIGAGLGVLQLILYALYYKRSPKDTSVVSVETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUL4</accession>
    <name>A0AAF1AUL4_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1 2">
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG95549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="18356" checksum="F8AEE97E2AA39C1A" modified="2024-05-29" version="1">MIMIGMAEANIVVDQLTSIQIDDDFEGLDDFFDIKDRSVNKQNLQRFRHQCRTSKKLLVLGIDGTIIDHRMKWIQLKLEEFWVLDNPDYKITTIFNRYAMISIRDIILEKYLFYKPSNTIVIYDLLRNFVMNPQNGLMTRHFRKAHARQADQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1V2</accession>
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            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">DCAR_0519850</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
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</entry>
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        </source>
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    </reference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042263140" evidence="2">
        <location>
            <begin position="25"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
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        </source>
    </evidence>
    <sequence length="177" mass="19032" checksum="E8096BE1222F6831" modified="2024-05-29" version="1">MAASGRSLSLAFSVLLVSMVLVSATDNDQSSLKSSSEKILTKVIGIQGIIYCKTGSQLIPIKDAIARLTCLAVDKKGYETAPFSILSKPADAKGYYFATLSPAELEQGWRLTKCKVFLEKSPLKSCNIPTDVNKGKTGTNLRSPRLLKTMNLYSIPAFVYTSNAPAAAAIPPVPRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <name evidence="8" type="ORF">DCAR_0728840</name>
    </gene>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51742"/>
        </source>
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    </evidence>
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    <sequence length="310" mass="32731" checksum="C1DC50CDDD601C91" modified="2024-05-29" version="1">MDPVTAHGRPLPPPFHARDLQLHYQPLQYQNQHQQQQQHNSDEGQSGSSGLSHGLKLERGEMIDNNNNNNTDGNELVSITGGRSDGEGSGNRKPRGRPAGSKNKPKPPIIITRDSANALRSHVMEIANGCDIQESVSTFATRRQRGVCILSGNGTVTNVTLRQPTAPGAVITLQGRFEILSLSGSFLPPPAPPAASGLTIYLAGGQGQVVGGSVVGQLIASGPVVIMAASFGNAAYERLPLEEEDQSAAPGNGALESPPGMIGGPQQQQQLMPEHSNSGTSLFHGLNPSMLNQLPSDQPYWAGAPRPPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Ashrafi H."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
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    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <end position="70"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="125"/>
        </location>
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            <end position="121"/>
        </location>
    </feature>
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            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH12443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="333" mass="36356" checksum="2FBFFA1A0017D08D" modified="2024-05-29" version="1">MIPYGGEAAVVADEDRAGEMRSVRGSWSNEEDAALTDLVTKFGPRNWSVLAQGVPGRSGKSCRLRWCNQLDPGLKRIPFTEEEDQLIISAHKRHGSKWSVIAKLLPGRTDNAVKNHWNATLKRRCLGLSKPGPASHNTLVDGLLETSRASSQASYGHPNFIKPPEVRKASMTPSGLVDFENVHDTVIPEMEEHHHVPEKEKDECRHNPEKDNPNSDSTLYRPVANVGAFAVYNTLGSMITKTTPGQRSLLQASRPEFGISKVLNYSCGDPVVPRLCGYGCCASHGEANCQSSLLGPEFVDYQEIPSSTNQELAALVTDLNNIAWIKSGVGTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BER4</accession>
    <name>A0AAF1BER4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BER4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF347">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="98" mass="11002" checksum="67BE2349FB694C51" modified="2024-05-29" version="1">MPEPCELGTSIRKSSLPSYLILIIPEADGVEVPSSVWRALLGACRIHNNLEVGKIAGKNVLKLESLNSGVYMILAEMYTASVKRLQAEKIWYERQKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XCK0</accession>
    <name>A0A161XCK0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0623935</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant DMP1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XCK0"/>
    <dbReference type="Gramene" id="KZM90383">
        <property type="protein sequence ID" value="KZM90383"/>
        <property type="gene ID" value="DCAR_022252"/>
    </dbReference>
    <dbReference type="OMA" id="AHKYMTL"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007770">
        <property type="entry name" value="DMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621:SF5">
        <property type="entry name" value="PROTEIN DMP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621">
        <property type="entry name" value="PROTEIN DMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05078">
        <property type="entry name" value="DUF679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH04526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="202" mass="22250" checksum="85157363FE8BFD9F" modified="2016-07-06" version="1">MASSSTIQVCTPRHPKPLHPVHRTLATAANLANLLPTGTVLSFRALTPSFSNQGHCQLSNKYLTAILIAVCAVACFLSSFTDSFVDSEGKLYYGIATFKGLNIFNYKNNNEAENELDMQDLRQYRLCFIDFVHAFMSLMVFLVFALSDSDVQSCYFPVGGENLNALVMNLPLGTGVLASFLFMLFPTTRRGIGVADLPLFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZMJ2</accession>
    <name>A0A161ZMJ2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_024584</name>
        <name evidence="7" type="ORF">DCAR_0727881</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZMJ2"/>
    <dbReference type="Gramene" id="KZM87450">
        <property type="protein sequence ID" value="KZM87450"/>
        <property type="gene ID" value="DCAR_024584"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193800"/>
    <dbReference type="OMA" id="IAMKIDM"/>
    <dbReference type="OrthoDB" id="510420at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045230">
        <property type="entry name" value="MBS1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213">
        <property type="entry name" value="GEO09665P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM87450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH08440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="106" mass="11114" checksum="E68655C35AAF795E" modified="2016-07-06" version="1">MTGKAKPKKHTAKELAAKLDAATTNRGGGKAGIADRTGSTKGGHAKYECPHCKITAPDVKSMQIHHDSKHPKIPFDDTKISNLHAGAPVVAETSAPRPGVRGSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZYD1</accession>
    <name>A0A161ZYD1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0100748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="143" mass="15755" checksum="3389C178A7E7EACB" modified="2016-07-06" version="1">MKKFFSELKLCYGGGSNTDSTAEDTTMTLPQPEKVVPLSIYYATGKKEIDTYASSGKISRRLKKLHSAANWKPVLSDILENNVIVSDVAFPRTRKQTTVVRSEVKASGKRKSAGSSASFYSDKYRKSTDPASLPAFSPTPYVF</sequence>
</entry>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN01261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG92050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="26247" checksum="8ECE11B6E19D1FBA" modified="2016-07-06" version="1">MVSPENTNWLYGLLDDIAVPDANFSVPTSSAFNWPVQALNNPLSVEIDGSKGNSSVVMEAGSKKRVRSESCSGSSSKACREKMRRDKLNDKFMELGAILDPGRPPRTDKAAILIDATRMVTQLRTESQKLKDTNSDLQDKIKELKNEKNELREEKQRLKAEKDKLENQLSTMNAQPSFMPPPSAIPAAYPQATYAAQAPVAGNKYVPIISYPGVAMWQFMPPASVDTSQDHVLRPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AMX0</accession>
    <name>A0A162AMX0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">PAR1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_012159</name>
        <name evidence="4" type="ORF">DCAR_0313779</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG94483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AMX0"/>
    <dbReference type="STRING" id="79200.A0A162AMX0"/>
    <dbReference type="Gramene" id="KZN03403">
        <property type="protein sequence ID" value="KZN03403"/>
        <property type="gene ID" value="DCAR_012159"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211921"/>
    <dbReference type="OMA" id="ERMSEYI"/>
    <dbReference type="OrthoDB" id="772928at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009489">
        <property type="entry name" value="PAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33649">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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            <begin position="25"/>
            <end position="194"/>
        </location>
    </feature>
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        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN03403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG94483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="20965" checksum="947DE79800E37C26" modified="2016-07-06" version="1">MASSNRLALILFLAYSFISQTSLGEIICEELPKDVCAFSISSSGKRCLLENSETKKGVEDQCSTSDVVVQGMSEHIETEACVNACGVDRRTIGISSDSLLEPQLTTKLCSPDCYHNCPNIIDLYFNLAAGEGVFLPDLCEKQKTNPHRAMLDLMNSSPAFAPSSNDEAEEYFVSPAPSSEVDENVEIAPAPSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162B686</accession>
    <name>A0A162B686_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_003189</name>
        <name evidence="3" type="ORF">DCAR_0103372</name>
    </gene>
    <organism evidence="2">
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        <lineage>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="DCAR_003189"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="133"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN10533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="133" mass="14043" checksum="ED03AF76390F4AF2" modified="2016-07-06" version="1">MSSPKDGDNMGKVGKGAPEPQQPRAVGPYRGLVIRNDDFSIGLGLDFTEPLNPWFLQKIEPKTHHPTNASEPRAPVCSEPGKENSLTPSPEMATKPLAFAPAVNGATCPNGVPNVKDSAAAENGSDIDLDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">F-box domain-containing protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">DCAR_004119</name>
        <name evidence="3" type="ORF">DCAR_0104398</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162B8W8"/>
    <dbReference type="Gramene" id="KZN11463">
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        <property type="gene ID" value="DCAR_004119"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN11463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG85210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="408" mass="46943" checksum="49B5B079007506EC" modified="2016-07-06" version="1">MAVKTKRKKTTAAELPDEILRHEILTRLPIKSAVRFKSVCKLWLFLISQPQFVFQHLTYNSTENPNAHDLLIADKGTKLVILSRYKETKLLPFDNLSFLVGSINGLVCLYRGTNLSLWNPAIHQSKEFRLPPGHFGVPGTSLGLAFDWVGNDFKVVALCADLRSASVYCSGLDDWSQVFFEEDLFPKTDFEDCTPPFIVKGCPYWTRSRYVYETKALVPGQEYTDLVEVFLSVVKFDPRNYEFTLSPEYHWDKDDIGIEFCHQFVDLKDRVTLIVHDQRAECCIVQMFTMDGEEGCSVWTKKCIVMLDFETRGMYVSLSQGFKFDGELVYHDNGMFACIVSETEENKRIRGTTSTEYQVTCYRYAPTLFFILGMKSVHLRTQTRTPHCHRTPRRLISSLRASSLSCDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U381</accession>
    <name>A0A164U381_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_025453</name>
        <name evidence="10" type="ORF">DCAR_0729264</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOH09805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A164U381"/>
    <dbReference type="Gramene" id="KZM88378">
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        <property type="gene ID" value="DCAR_025453"/>
    </dbReference>
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    <dbReference type="OMA" id="GMIPGWM"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:carpel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MADS-box transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor, MADS-box"/>
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    </dbReference>
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        <property type="entry name" value="MEF2-like_N"/>
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        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
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        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
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        <property type="entry name" value="TF_MADSbox_sf"/>
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        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="88"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM88378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH09805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="251" mass="29029" checksum="CA7D3B1A0B862286" modified="2016-07-06" version="1">MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFCSTSNMLKTLERYQNCSHGTLEVNRTAKDLEQNSYREYLKLKGKYDSLQQYQRHLLGEDLGPLNIKDLEHLELQLDESLMHIRSTKTQNMLDQLSELQNKERILLETNTALEEKLREYHSETQLRQLWPGGEVSASYGQQQHHQHQQQQGQSQGFFQPLNCNSTLQIGYNAVGSDQMNGTNGQNVNGLVPDWML</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UKQ2</accession>
    <name>A0A164UKQ2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0729960</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_026082"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193429"/>
    <dbReference type="OMA" id="GCNDAAV"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000067">
        <property type="entry name" value="calcium-dependent protein kinase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH10491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="544" mass="61553" checksum="6CF4BEE842A9B322" modified="2016-07-06" version="1">MGNCVTCITPDTKTHHNTTPTPNTTNKKAKKQNPYSTSPALIRVLKDISPGGSRWTRVTDKYTLGHELGRGEFGITHLCTDRETREKLACKSISKKKLRTAIDVEDVRREVAIMSSLPMHSNIVKLRATYEDGEAVHLVMELCEGGELFDRIVARGHYSERAAAAVAKTVAEVVKMCHDNGVIHRDLKPENFLFANKKETSPLKAIDFGLSVFFKPGEKFSEIVGSPYYMAPEVLRRNYGPEVDVWSAGVILYILLCGVPPFWAETEQGVALAILRGNLDFKREPWPQISDNAKSLVRRMLEPDPKKRLTAKQVLEHPWIQNAKKASNVPLGDIVRTRLKQFSLMNRFKKKALRVIAEHLTIEEVEVIRDMFTLMDTDNDGKVTYEELKAGLRKVGSQLAEPEIRMLMDVADVDGNGVLNYGEFVAVTIHLQRMENDEHIRRAFTFFDKDANGYIELDELREALADESGETDDNVLNEIMREVDTDKDGKISYDEFVAMMKTGTDWRKASRQYSRERFQSLSVNLMKDGSLHLQDAISGQTFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WD08</accession>
    <name>A0A164WD08_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lunapark zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_021019</name>
        <name evidence="5" type="ORF">DCAR_0625311</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WD08"/>
    <dbReference type="Gramene" id="KZM91616">
        <property type="protein sequence ID" value="KZM91616"/>
        <property type="gene ID" value="DCAR_021019"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226396"/>
    <dbReference type="OMA" id="MRTFRIL"/>
    <dbReference type="OrthoDB" id="1725934at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="3">
        <location>
            <begin position="290"/>
            <end position="340"/>
        </location>
    </feature>
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            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="344"/>
            <end position="411"/>
        </location>
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        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
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        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
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            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM91616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH05888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="411" mass="45668" checksum="C339300A6FD5AF34" modified="2016-07-06" version="1">MATDSKKDQKEVAEIAGDLSATASEEKTMKKKKRGILSRIWNWVFRLHGDDFEKRLQHISKEEAAIVARLKRRSINRRGVTKYLIIFSVLLELFAVCYAIMTTRSLDLNWKMRALRVSPMFLLPVLSSLSYSAIVSFTRMRDRKDQKTLERLRAERLEKINELKEKTNYYITQQLIQRYDPDPAAKAAAATILASKLGADSGLKLYMGDDSKLNPSTGKSSDVENVQSSGLRNRKQQLSRSSSGSSAVMQQLEGEMMQHAATEGSDMARHDQMVVEHENPTSLHAQDGGWIARIAALLVGEDPTQSYALICGNCHMHNGLARKEDYPYITYYCPHCNALNRPKQLEGSSPPGSSLVSSPISGPLKPVDEVSVDQNVSKPTSDRASDSSSPVAAAVENREATETRVCEGEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YJ73</accession>
    <name>A0A164YJ73_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_017837</name>
        <name evidence="2" type="ORF">DCAR_0520444</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YJ73"/>
    <dbReference type="Gramene" id="KZM94594">
        <property type="protein sequence ID" value="KZM94594"/>
        <property type="gene ID" value="DCAR_017837"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM94594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH01065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="86" mass="8731" checksum="3254B3CC763FEDBD" modified="2016-07-06" version="1">MDGRKPGYSSQVFNSTETALDTMVGTGVLANDVLQSIILGVIADLLPKLGCLKAPVSGSGAADGMLGMEQAAEIMKHGKTAKKAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YKK6</accession>
    <name>A0A164YKK6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0520503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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            <dbReference type="EMBL" id="KZM94708.1"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Zheng Z."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="12">Leaf</tissue>
        </source>
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        <location>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z9L5"/>
    <dbReference type="STRING" id="79200.A0A165Z9L5"/>
    <dbReference type="Gramene" id="KZM99778">
        <property type="protein sequence ID" value="KZM99778"/>
        <property type="gene ID" value="DCAR_012860"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218852"/>
    <dbReference type="OMA" id="LFYARCT"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF874">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="15">
        <location>
            <begin position="80"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="602"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="179"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="244"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="915"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="491"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="931"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZM99778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG98904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1154" mass="126474" checksum="301602700304A5ED" modified="2016-07-06" version="1">MLGRGDLGFVAVVAAVFAVIGIVIRRKWRTAAARRREVERLLVFAAEEAERAELEASVQYGAAVAKEESVSEKLVDRSECALCFSVATKFCSRCKAVRYCSGKCQIIHWRQGHKQECRPYSIVKKGNEEKFSCQKEFNQEEPRNYGDSYELDKSQYAKQVETSPGTYTDVGRSDLLHSKDDIEGEGLTVDAGSNLSEGSSEPTCTSSKASLDASVYDSDGLTRIQSAIDVLLKSESACVDMDRNRSPFAETSSNLKKSSIKSVQYKPDSSDGDSHSFSSSTSGWSVDGSSDSLSEPYTPSSGFWEGNINSRRSRNGANYDGTQSILSDFGGDVMLSPVSSMRYSADMAKSLTRGMPQQGFNSNTMISNGGPSASRTKRYVSDATSSVKLCKDNLKSKSLNLETSASMHVETGCNSRVLNSRETKSSSSDNHCARPCANDQGHLIMKDFKNISNSPSLCSERSNKVRNDTISSSHALESQEVGSVLCRDSDTGLTSSTSRDAGLTSSTSRDAGHIANLSKVSDGVHGFAGSSSKSPINSTSARNGLKSTMLKVVDQLKPSKISKPYSTGLDSGTVGKYNVKGLFSYELFVKLYNWNKVDMQPCGLINCGNSCYANAVLQCLAFTPPITAYLLQRLHSKTCQNKDWCFTCEFENLIVKAREGSTPLSPIRILSHIENIGSHLDYGKEEDAHEFLRCAIDTLQSSCLKEATSKISGSLNEETTLMGLTFGGYLRSKIKCMKCGGKSERNDRIMDLTVEIDGNICTLEEALRKFTSTEILDGENKYNCSRCKSYEKAKKKLTIIEAPNVLTIALKRFQSGKFGKLSKSIEFSEILNMAPFMSSTSDTSPIYRLYGVVVHLDIMNASFSGHYICYVKNIQNKWFKIDDSSVNAVELESVLTKGAYMLLYARCSPRAPKLIRNSMVPRDPRKPKNPSLKSRSHSTGLLDSPRTNHLNYQTGEGFYQTHPSFQRVSSVLEDDSSSDNSSSIFSEAASCSTESSTKDSMSADDYFDQIFGDFGHKLNSSWWNTSDSDTSSSSSSPSPLYTKNIPYSSSDHYVPGYNEENHCSADPAASAEDGRVWANQPGKSSNLDSLKGKGSTPILYSDKTKQCREVVDSNSDFSCSRCRATNLNSIGKINPLNNVKSGISHRRYSRGQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZEL1</accession>
    <name>A0A165ZEL1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_008693</name>
        <name evidence="7" type="ORF">DCAR_0309815</name>
        <name evidence="8" type="ORF">DCAR_0832409</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZM99938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZEL1"/>
    <dbReference type="STRING" id="79200.A0A165ZEL1"/>
    <dbReference type="Gramene" id="KZM85430">
        <property type="protein sequence ID" value="KZM85430"/>
        <property type="gene ID" value="DCAR_027148"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM99938">
        <property type="protein sequence ID" value="KZM99938"/>
        <property type="gene ID" value="DCAR_008693"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197064"/>
    <dbReference type="KEGG" id="dcr:108214329"/>
    <dbReference type="OMA" id="RFRENAM"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018262">
        <property type="entry name" value="Ribosomal_eL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01107">
        <property type="entry name" value="RIBOSOMAL_L27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KZM99938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG90571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="135" mass="15519" checksum="82A0C0B9E45A29C6" modified="2016-07-06" version="1">MVKFLKPNKAVIVLNGRFAGRKAVIIRAFDEGTRDRPYGHCLVAGISKYPKKVIRKDSAKKTAKKSRVKAFIKLVNYNHIMPTRYTLDVDLKDVVTADCLQSRDKKVTAAKATKAKLEERFKTGKNRWFFTKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZVR9</accession>
    <name>A0A165ZVR9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0310454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZVR9"/>
    <dbReference type="Gramene" id="KZN00492">
        <property type="protein sequence ID" value="KZN00492"/>
        <property type="gene ID" value="DCAR_009246"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210846"/>
    <dbReference type="OMA" id="NTACIIA"/>
    <dbReference type="OrthoDB" id="78663at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17338">
        <property type="entry name" value="MFS_unc93_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044771">
        <property type="entry name" value="UN933_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF13">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG91206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="432" mass="46839" checksum="CBECAFC8920F0AD3" modified="2016-07-06" version="1">MDEEVALIAQTKTPLSHTRDVHFLSWAFLFIFLAYGAVQNLESTINTEKNLGTISLGVLYTSFTVSTLFASFVVRMLGSKNALVLGTIGYWLFIAANLFPTWYTMVPASLYLGFSASIIWVGQGTYLTSTARSHAYDHNEHEGTIIGQFNGEFWGMFASHQLVGNLLSLVLLNKGEDGSTSGTTLLFVVFLSSMTVGTILMCFLHKRDPKKEEGLQESSVTFYSLMVSLSNSVINPLCDRRLLLIIPLIAYSGLQQAFVWAEYTRFIVEPVLGEAGVGGAMAVYGAFDAICSLAAGRLTSGLSSITLIVCGGALTQAIVLLWLLLGYSTSTGLLGTVIPLIIAAIWGIGDGIFNTQLSALIALLFKHDMEGSFAQLKLWQSASTAVVFFVSPYITFNVMLEFMLVAVCISVAGFLVLTLKLEKAFSSNTSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BN63</accession>
    <name>A0A166BN63_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_011405</name>
        <name evidence="9" type="ORF">DCAR_0312905</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN02651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166BN63"/>
    <dbReference type="STRING" id="79200.A0A166BN63"/>
    <dbReference type="Gramene" id="KZN02651">
        <property type="protein sequence ID" value="KZN02651"/>
        <property type="gene ID" value="DCAR_011405"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213774"/>
    <dbReference type="OMA" id="YQGHQNK"/>
    <dbReference type="OrthoDB" id="1917746at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF72">
        <property type="entry name" value="NDR1_HIN1-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="142"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN02651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG93619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="269" mass="29738" checksum="C49B54C9585A25DE" modified="2016-07-06" version="1">MADHLKIHPVVDIEAPPTAPLVANSSSKASERGGSLLHVKDMNPAPPRAQPPPGPPPPQFRQTTPLIPSPPPRRKSRSFCCKCICWTISLLILILIILGAIVGILYLVFQPKIPKYSVDRLQISDLRLNTDGSLYAKFDVQITAKNPNKKIGIYYQQGSHLSVWYAKTKLCQGKFPKFYQGHMNKTRLDVSLTGQAQYGNTLLGALQEQQQTGRIPLDLKVDVPVSVKLGKLKLRKVRILGKCLLIVDTLSSNSVITIKASSCKFKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D112</accession>
    <name>A0A166D112_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_005433</name>
        <name evidence="2" type="ORF">DCAR_0206122</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D112"/>
    <dbReference type="STRING" id="79200.A0A166D112"/>
    <dbReference type="Gramene" id="KZN04596">
        <property type="protein sequence ID" value="KZN04596"/>
        <property type="gene ID" value="DCAR_005433"/>
    </dbReference>
    <dbReference type="OMA" id="IPASTIC"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF38">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE REF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN04596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG86903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="183" mass="20606" checksum="4555C2B39A438DE7" modified="2016-07-06" version="1">MKTTYVQLNKSLAACSASPEGELRPTFTTRVQQVGSCPGKGHPVIKSVRESGKSYTVEEFEAKAKSFERNYFKKSSIDKGALSPLEIESLYWEADANKPFEVEYANDMHISAVVELEKRRGEDGLSDDLNVGDTDWNLRGAARSRRCLLRFVKDDIPASTICTLVRGRPGMECLRMQQQHLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D1N0</accession>
    <name>A0A166D1N0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206154</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D1N0"/>
    <dbReference type="Gramene" id="KZN04621">
        <property type="protein sequence ID" value="KZN04621"/>
        <property type="gene ID" value="DCAR_005458"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207311"/>
    <dbReference type="OMA" id="VCAIYYH"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016135">
        <property type="term" value="P:saponin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1560">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 74E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG86935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="458" mass="51378" checksum="1D8B1646B9ECC3CB" modified="2016-07-06" version="1">MGEIDRELACKTHVMIIPFPAQGHMSPMMQFSKRLAFKGVKVTMVVPLKLQDSMQILYDSPLISLECITFDFGKDEIPNDMQSYMGFMKLKITTVLPELLEKQENNGCPVKFLVIDSLFPSGVEMCHQFGLRGAPFFTQSCAVNAIYLNVLQGRLKIPFDKGLSNFSLPSLPVLDTVDMPSNVIGTFPDLWAYFSRQFSGLEKADWVFLNSFDKLETEEAKWLASHCPLTTVGPAIPSMYLDKKLPNDKKYGISLYKPEAEVCLNWLDERDPGTVVYISFGSVDKLSQEYMEEIGRGLLKSNSFFLWAIRDSEREKLSSDFISESSEKGLIVSWCPQLDVLAHRAVGSFMSHCGWNSTLEALSLGVPVVAVPVWFDQPTNGKYIVDIWRVGVRVPVDESGMVSREDVAACIMDAMEGDSKEEIRENALKWRQVAIEAMDEGGSSDRNIDDFISKLVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166D2L3</accession>
    <name>A0A166D2L3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_005481</name>
        <name evidence="3" type="ORF">DCAR_0206178</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166D2L3"/>
    <dbReference type="Gramene" id="KZN04644">
        <property type="protein sequence ID" value="KZN04644"/>
        <property type="gene ID" value="DCAR_005481"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN04644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="90" mass="10469" checksum="F82979EDC98C072D" modified="2016-07-06" version="1">MGSQSPSKTIRIIADKSVLEERIKCLVEEIANLQENLRLMENQLAHHQIVIDLMKKQIEERTAREDFIPVEVESRKASKLVEAERKEDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FJP2</accession>
    <name>A0A166FJP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_000524</name>
        <name evidence="5" type="ORF">DCAR_0100292</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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            <tissue evidence="5">Leaf</tissue>
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                <person name="Senalik D."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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    <sequence length="200" mass="22234" checksum="CE4E34A229831464" modified="2016-07-06" version="1">MAKPALGPERGTNPVIWFAAIILAIVAVAVITIGLFVIISYLVIHPTTPSISVSYANLDKFDYDQLGMLDVQVRVVVKAKNGNIKNHVRFYGLELVLALHGLQMAKLVNEPFDVKKNDSVEFLYVVKSNKIPLRSVYRDYVQSSLMKNLVSLELKGTTKTRWKVWVVDSVKITLHMDCDLHFVVPNGSLSTGSRCSSKSS</sequence>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
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                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="15">
        <location>
            <begin position="67"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="240"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="402"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZN10509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG84166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="61407" checksum="66290EADE789CD74" modified="2016-07-06" version="1">MGIVSTVLGFSGFGVGVSAGLVIGYYLFIFFQPTDVKDPQIRPLVEQDTKSLQRLLPEIPHWVKNPDYDRVDWLNKFLEYMWPYLDKAICKTAKNIAKPIIAEQIPKYKIESVEFEALTLGSLPPTFHGMKVYVTDEKELIMEPAFKWAGNPNVTVAIKAYGLKATVQVVDLQVFASPRITLKPLVPSIPCFANIYVSLMEKPHVDFGLKLLGADLMSIPGVYRFVQELIKDQVANMYLWPRTLEVPVMDPAKAMKRPVGILHVKVLRAMKLKKKDLMGASDPYVKLKLTEDKLPSKKTVVKHKNLNPEWNQEFHMVVKDPESQAIEFTVYDWEQVGKHDKMGMNVVSLKELSPDEPKELTLDLLKNMNPNDPINEKSRGQLVVELTYKPFKEEDMPKDFDESGEVEKAPEGTPSGGGMLVVIVHEAQDVEGKHHTNPFARILFRGEERKTKHVKKNRDPRWEEEFSFVLEEPPINDRLHVEVLSSSSRIGILHPRESLGYIDISLADVVHNKRINEKFHLIDSKNGRIQIELQWRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HXW7</accession>
    <name>A0A166HXW7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103361</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HXW7"/>
    <dbReference type="Gramene" id="KZN10522">
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        <property type="gene ID" value="DCAR_003178"/>
    </dbReference>
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    <dbReference type="OMA" id="CECERIC"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="130" mass="13978" checksum="05FC96C28E1B0610" modified="2016-07-06" version="1">MATARIKELFKKYGKVALGVHLSVSAASVTGLYVAIKSNVDVESLFERFGIGVAKEDEEAVKITENMNDEIINPKKRNRTAELAASSGGALAVAVLCNKALFPVRVPITLALTPPISRFLARRRLLKGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HYQ8</accession>
    <name>A0A166HYQ8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0103394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HYQ8"/>
    <dbReference type="Gramene" id="KZN10552">
        <property type="protein sequence ID" value="KZN10552"/>
        <property type="gene ID" value="DCAR_003208"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205250"/>
    <dbReference type="OMA" id="FYKHAYH"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16521">
        <property type="entry name" value="RING-HC_MKRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1350">
        <property type="entry name" value="Pneumovirus matrix protein 2 (M2), zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG84212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="359" mass="40285" checksum="10AF5125B275CA24" modified="2016-07-06" version="1">MSKRVLCKFYSHGACLKGENCEFSHDWKDPANNVCTFYQKGVCSYGSRCRYEHVKVSRSQPCATSSSAVTLQYSGSFPATNSSRNAASGRTAAEGVNRELLDSSRSFVPATNPAWNEISALPQSLDDTYTRESGTNPAELVMCSFAAAGNCPRGEKCPHIHGDLCPTCGKHCLHPFRLEEREEHSRMCEKRHIHLEALKRSQEIECCVCLERVLSKPTAAERKFGLLSECDHPFCVSCIRNWRSSSPISGMDVNSALRACPICRKLSYFVIPSVIWYSTKEEKQEIVDSYKSKLRSIDCKHFDFGEGSCPFGTSCFYKHAYRDGHLEEVDLRHLGADDGMTVIAKDIRLSDFLNSLRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J3S5</accession>
    <name>A0A166J3S5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_004417</name>
        <name evidence="6" type="ORF">DCAR_0104776</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Exo70"/>
    </dbReference>
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        <property type="entry name" value="Exo70_C"/>
    </dbReference>
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        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF7">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
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    </dbReference>
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    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
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        <location>
            <begin position="208"/>
            <end position="569"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN11761.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85585.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="589" mass="67520" checksum="CCE9D4FE23C2778A" modified="2016-07-06" version="1">MADDLHIPLVAFQEMKDSNSKCEDTNVPDLKTAKKVIEELGYPFQLQQLINNGDREKFNQYLYAVVEIQQSINSGLITGYETHGSRAIKSLHLVFRGILDCSISANTSSDPSSSSLYSSGVPSTYSYELQGRNRTFEYELSSEKIYYLRSIVEKLNAIECLGDCIDVYKNLRRSAVDAKYQWFRIGNWTTTDLKKLDSRQFAAKINIWIQVAKIFYDGFFVGERLSFEQIFKGVTAATYHNCFVPIVEHVAVELNNFASAVSSIASFQNLFPVLDLYSALDVILPDILIMFSTSWTINISQGAINIRRSLLTLVGKLLCSFEDTVQNELSTALLPEGTVHFLTAYAMEYVTKISLHKELLRYMIESPTKSLGNQADDQFLNSAGGTPLALRVVWIIISLRINLERKSSHYRDAWCRYVFVTTNVNYVIKTIKSSPELLDLIGKEYSSKLSTYIAQARQDYSSTIWDRVLYCLRDDGLKHKFLFYNRISRNSVKNRFKTFNTTFEEACRSQSRLFVPDIDIDSQFHMLILSKLLPAYKVFLEKYSSHLQSEKYKERCIKYSSEDLQSKIQTLFTEKLIVHNGTEENLMIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
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    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="445" mass="52440" checksum="EFC0D5B13BA4A074" modified="2016-07-06" version="1">MVTKLIKIQTLSLIKANNSITSTPIPHFSFIRSMTTSKRVQDRSEKKRIHDLEIATEKWKIISKVLFLIETMKQEPEQVISVRSLDQYRRQINLPKPHKISDFLRKSPKLFELYKDQRGVLWCGLTRQAEELVEEEERILEVTSEKAVEYVSRLLMMSIDKRLKLEKIAHFRRDLGLPYDFRKNWVYKYPDNFRVVKDEDEVEFLDLVSWNPCWAITELEKNVLGLSTLDESVKDHVPGLLSLAFPMKFPPNYKKIYRYGGQIDHFQKRVYLSPYADARELKAGSQEFDKRAVAVMHEMLSFMNEKRLVTDHLTHFRREFVMPQKLMRLLLKHFGIFYVSERGKRFSVFLTEAYEGSELIEKCPMAIWKEKVQKLIGYRGRKKKIETFDDLPDMEDRDLFESDSEEDIISIAPVHDETLNAYNDASHSDNSEMEIGDVCSAYKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1 2">
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBM7"/>
    <dbReference type="Gramene" id="KZM80628">
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        <property type="gene ID" value="DCAR_031902"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="259" mass="29221" checksum="E81EF040DAE55FF9" modified="2016-09-07" version="1">MISLRMTLLALKRLRSILIRNKLAENEFKLKAYKNSSQVVQDIYEKITKNNKLRIGYEYGRRPGKKTVSDYVGDDTVKPLILRKTAHTKNAVNKAATESFRGTVKRKRKKVTFVTAVKTVEANAAVKGHPTATFECVVNTNDTKSKNRNGKINRGKNDEYPKNAPRKLCNNFGCSHHLTHICRNDAATRINAVNVHGDLHRTPIMDRSMNVCSNIDCMSCKITAMSTVFNLPILSTAKCSHLYDVETLEATKVSFQARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YBP9</accession>
    <name>A0A175YBP9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0205629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YBP9"/>
    <dbReference type="Gramene" id="KZM81046">
        <property type="protein sequence ID" value="KZM81046"/>
        <property type="gene ID" value="DCAR_031378"/>
    </dbReference>
    <dbReference type="OMA" id="KGVGDEF"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF6">
        <property type="entry name" value="COPPER-TRANSPORTING ATPASE RAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="94" mass="10116" checksum="97B88ACEE04B26D1" modified="2016-09-07" version="1">MARSVELTRKDSPAGDLEDVRLLDSYEDEEAELSKVEQGMSRIQVRITGMTCAACSNAVETALISLNGVVSASVALLQNKADVVFHPSVVNVCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YE56</accession>
    <name>A0A175YE56_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="6">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_029193</name>
        <name evidence="19" type="ORF">DCAR_0933570</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="6"/>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YE56"/>
    <dbReference type="STRING" id="79200.A0A175YE56"/>
    <dbReference type="Gramene" id="KZM81580">
        <property type="protein sequence ID" value="KZM81580"/>
        <property type="gene ID" value="DCAR_029193"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203149"/>
    <dbReference type="OMA" id="ELPLMIM"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000005">
        <property type="entry name" value="Bifunctional riboflavin biosynthesis protein RIBA 1 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10990:FF:000001">
        <property type="entry name" value="Riboflavin biosynthesis protein RibBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01283">
        <property type="entry name" value="RibBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016299">
        <property type="entry name" value="Riboflavin_synth_RibBA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006803">
        <property type="entry name" value="PRK09311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF48">
        <property type="entry name" value="BIFUNCTIONAL RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="17">
        <location>
            <begin position="336"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KZM81580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH14054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="552" mass="59941" checksum="2DA931F47E68FE14" modified="2016-09-07" version="1">MAAMNMSGPSSAFPRLQTQKVFSLSNAQLVNLTEANIFTSDSSSAVGSRSGFCIKAINKVNSTLPSGSRGLYSHPKSWRSTLHCSSSAGIEVQPDSIALGTLSADTSPSGSIFPVDSNEYDLDHPTVGLSSIPQAIEDIRQGKMVVVVDDEDRENEGDLIMAASKVTPEAMAFIVKHGTGIVCVSMKEEDLERLQLPQMVTHKDNEEKLTTAFTVSVDAKKGTTTGVSARDRATTILTLASKDSKPEDFNRPGHIFPLRYREGGVLKRAGHTEASVDLTVLAGLEPVSVLCEIVDEDGSMARLPKLQEFAKKENIKIVSIADLIRYRRKRDHLVERASSARIPTMWGPFTSYCYRSIIDGIEHIAMVKGEIGEGVDILVRVHSECLTGDIFGSARCDCGNQLGLAMQQIEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEELGLPVDSREYGIGAQILRDLGVRTMKLMTNNPAKYIGLKGYGLAVSGRVPLVTLITKENKRYLETKRAKMGHVYSQGFNSNVINVVTEDGKPSTGEQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFK1</accession>
    <name>A0A175YFK1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_029663</name>
        <name evidence="4" type="ORF">DCAR_0934175</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFK1"/>
    <dbReference type="STRING" id="79200.A0A175YFK1"/>
    <dbReference type="Gramene" id="KZM82050">
        <property type="protein sequence ID" value="KZM82050"/>
        <property type="gene ID" value="DCAR_029663"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200841"/>
    <dbReference type="OMA" id="PTPEWNK"/>
    <dbReference type="OrthoDB" id="1916780at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF20">
        <property type="entry name" value="THYLAKOID LUMENAL 19 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="2">
        <location>
            <begin position="95"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM82050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="26009" checksum="0B5CBCE65BC5FC47" modified="2016-09-07" version="1">MASTFSPSALLSTTTSTSSTPSKPPQFKPQLTIPPSKPSSNNLTTTLTAALAAATILTSTTPSLADSTYQTYYGTAASAANYGGYGGNSNKKDSAEYIYDVPEGWKERLVSKVEKGTNGTDSEFYNPKKKTEKEYLLYLAGIRQLGPKEAILNNLALSDVDLQDLISSADSVTSEERNDDKGQVYYVYEIDGVAKHSLISVTCARNKLYAHFVNAPTPEWNKDKETLRHVHESFKTIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGB9</accession>
    <name>A0A175YGB9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_029485</name>
        <name evidence="2" type="ORF">DCAR_0933977</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGB9"/>
    <dbReference type="STRING" id="79200.A0A175YGB9"/>
    <dbReference type="Gramene" id="KZM81872">
        <property type="protein sequence ID" value="KZM81872"/>
        <property type="gene ID" value="DCAR_029485"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM81872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH14458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="111" mass="12805" checksum="2B27E39ABB817876" modified="2016-09-07" version="1">MTLEGPFKPVTIPLDETFRGNDIDLPDNDPRVVRIVNGFQPEQISLSLSSTFDSVWVSWVTVHKFTKVLRECRIVDEMTSARFSTKFHKGYYTWIPSAMRDQVFMVIGYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGS9</accession>
    <name>A0A175YGS9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0934945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGS9"/>
    <dbReference type="Gramene" id="KZM82567">
        <property type="protein sequence ID" value="KZM82567"/>
        <property type="gene ID" value="DCAR_030136"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH15407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="171" mass="19054" checksum="9D450B18498B95A0" modified="2016-09-07" version="1">MVNIEEIPVICADVHRHVDSFESDDSVVSSDNENSEFEIDAKQVSENNKADPTYQVGADGTKYWTSKCDEKKKPQLNQHFTTLDEISRSSKIDGNAHRVDNFSGPVPEGEISMQNLDISRNKGCGSCLKSSRELGAQDEKKKKKCGNCNQLVSRNARRTCPEPPKNIVEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGT8</accession>
    <name>A0A175YGT8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGT8"/>
    <dbReference type="Gramene" id="KZM82391">
        <property type="protein sequence ID" value="KZM82391"/>
        <property type="gene ID" value="DCAR_029960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH15206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="16194" checksum="74A810CB423250C8" modified="2016-09-07" version="1">MKAAKEDFALMCKSMTDRQAEILTSGLFLEEQHRRVIDLLDGKQDVVGDSSTADDETARTSGNEDARKLAREADESLRNHRVYREIELQELEKIFKKYEGMLKQNMSEMRKSAEDIEIQRRELSPKLIQLARSKADESGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGY1</accession>
    <name>A0A175YGY1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0935372</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGY1"/>
    <dbReference type="Gramene" id="KZM82879">
        <property type="protein sequence ID" value="KZM82879"/>
        <property type="gene ID" value="DCAR_030448"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108201571"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000107">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF1">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH15826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="305" mass="35176" checksum="42D35BA850CEF67A" modified="2016-09-07" version="1">MSKKASYKDARREINSKEVDEQMINSCDDQEEETMIEDLRRGTWTAEEDMALMNYISHHGEGRWNSLAHSAGLKRTGKSCRLRWLNYLRPDVRRGDITLEEQLMILELHSHWGNRWSKIAQQLPGRTDNEIKNYWRTRVQKHAKQLKCDVNSKQFKDFMRYVWMPRLVERIQAARGDSTGKATTGNSTFAKATDSNDNINNLSVPSQVIIMRETDSMMCDNLESMVQSETNNPSSLPPENISTTASLTPDSCTNLFLDLIRVMLGPQKLIQKYAQNDVDSTIICAMSFFVYRCGFHSHQCYAIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YHL7</accession>
    <name>A0A175YHL7_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0935533</name>
    </gene>
    <organism evidence="1 2">
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        <lineage>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YHL7"/>
    <dbReference type="Gramene" id="KZM83013">
        <property type="protein sequence ID" value="KZM83013"/>
        <property type="gene ID" value="DCAR_030582"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202772"/>
    <dbReference type="OMA" id="CPELEWI"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
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    </dbReference>
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        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF41">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH15984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="603" mass="65572" checksum="74A4AA00ED3D492D" modified="2016-09-07" version="1">MRGRDWINLILPDELILEIFDHLDDKSSRDACSLVCKRWCSLERLSRDTIRIGASGRPDELVRILSRKFVNVKNLYVDERLSIPLPVESGRRRPSVLSVRLHLLSETSGSQDNETEAYCLSDAGLAAIGDGFAKIEKLSLIWCSNATSAGLKSMAEKCKFLKSLDLQGCYVGDQGLGAVGECCKKLEDLNLRFCEGLTDAGLVELAIGCGSTLKSIGVAACAKITDISLEAVGSNCKCLESMALDSEFINNRGVLAVAKGCLKLKELKLQCINVTDEALQAVGLFCLCLELLSLYSFQRFTDKSLYAIGKGCKKLKNLTLSDCYFLSDKGLEAVATGCTELSHLEVNGCHNIGTFGLETIGRSCRQLSELALLYCQKIGNFALSEVGRGCKYLQALHLVDCSSIGDDAICSIARGCKLLKKLHIRRCYLVGNRGIIAVGENCKFLTDLSLRFCDRVGDSALIAIGRGCSLHHLNVSGCHQIGDAGIIAIARGCPKVSYLDISVLQNLGNMAMAELGEGCPLLKDIVLSHCRQITDVGLEHLVKRCPLLETCHMVYCPGITAAGVATVVSSCDKMKKILVEKWKVSQRTRRRAGSIISYLCVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQS1</accession>
    <name>A0A175YQS1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <shortName evidence="16">DHOdehase</shortName>
            <ecNumber evidence="4 16">1.3.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_026881</name>
        <name evidence="20" type="ORF">DCAR_0832697</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="16">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="4 16"/>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQS1"/>
    <dbReference type="STRING" id="79200.A0A175YQS1"/>
    <dbReference type="Gramene" id="KZM85697">
        <property type="protein sequence ID" value="KZM85697"/>
        <property type="gene ID" value="DCAR_026881"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108197323"/>
    <dbReference type="OMA" id="IYGTDTR"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000066">
        <property type="entry name" value="Dihydroorotate dehydrogenase (quinone), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 16" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="18">
        <location>
            <begin position="114"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KZM85697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH13188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="449" mass="47788" checksum="72E7A1BB0C8E54AC" modified="2016-09-07" version="1">MWARVGVKFMRQNLGQKLNLGSRFYSAAARPKLPQSSRKGRLLTGATIGLLIGGGAYASTVDEATFCGWLFSATKVVNPFFALLDPEVAHRLAVAAASRGWVPREKRPDPSILGLEVWGRRFSNPIGLSAGFDKNAEAVDGLLGIGFGFVEVGSVTPVPQDGNPKPRIFRLKSEGAIINRCGFNSEGIVAVAKRLGAQHGKRKLDETSNTSSSSSDEVKQGGKAGPGILGVNLGKNKTSEDPDADYVQGVHTLSQYADYLVINVSSPNTPGLRQLQGRKQLKDLVKKVQAARDEMQWGEEGPPPLLVKIAPDLSKQDLEDIAAVALALRLDGLIISNTTISRPDSVSKDPLASESGGLSGKPLFNLSTDILKETYILTKGRVPLIGCGGISSGVDAYKKIRAGASLVQLYTAFAYGGPALIPQIKAELAECLEKDGFKSIYEAVGADCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5P4</accession>
    <name>A0AAF0W5P4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0101781</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5P4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052610">
        <property type="entry name" value="bHLH_transcription_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45959:SF2">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8603" checksum="95977A8885741E12" modified="2024-05-29" version="1">MDKASVLGDVIKYLKQLQERVKALEEQTRRNIDNHAAGIPVEGPLPEIEVRIPYKDILIKIHCEKRKGVLKEKPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5R9</accession>
    <name>A0AAF0W5R9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0102884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5R9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044567">
        <property type="entry name" value="CLSY/DRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN CLASSY 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821:SF5">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN CLASSY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG83707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="159" mass="18041" checksum="280887AC78DB6868" modified="2024-05-29" version="1">MDGKLEAKQRQSSISALNDPKSKSRVLLASIKACSEGIHLVGASRVVLLDVVWNPSVERQAISRAYRIGQTKIVYTYHLIAEDMEVRKYKVQTAKDRLSEMLFSSKDTDSLQKNSPDMVSEDRILQEMYQQNDKLGGLFKQILYQPKESDLIDTFDVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W988</accession>
    <name>A0AAF0W988_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0104740</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Alfin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W988"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF98">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="199"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="145"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG85549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="255" mass="29110" checksum="17272DB19EB69E29" modified="2024-05-29" version="1">MDGGQQYNPRTVEEVFRDFKSRRAGMIKALTTDVEDFFQQCDPEKENLCLYGFPNERWEVNLPAEEVPPELPEPALGINFARDGMQEKDWLSLVAVHSDAWLLAVAFYFGARFGFDKADRKRLFNMINDLPTIYEVVTGAAKKQSKEKSAVSNHSSSKAKPNPKQRVTESQREVPKSLQLKDDDEDEEGLEEEEDEHGDALCGACGENYGQDEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPSCSNKRART</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAC8</accession>
    <name>A0AAF0WAC8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0104984</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endomembrane system</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAC8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG85791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="214" mass="24145" checksum="F0C693C971377F96" modified="2024-05-29" version="1">MALCSDYLFKLLLIGDSGVGKSCLLLRFAVSAFSSIRCFIYIKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVCNRYLCFLAPFPIKEYKKSSNFICTLWQVVYDVTDQESFNNVKQWLSEIDRYASENVNKLLVGNKCDLTSQKVAFADEIGIPFLETSAKSATNVEQAFMAMTADIKNRYSMPPTVQIRGQPVNQKSGCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAJ4</accession>
    <name>A0AAF0WAJ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0105074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAJ4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF66">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="195" mass="22006" checksum="180F088503CA5C51" modified="2024-05-29" version="1">MDVTIVSAQGLRSRTNLFSSRGIRPFTTLSTNINGVKHLHVYRTKVDNYGGANPAWGDKFQLPVDEATFNYSNVISSYIYLEVFTKRLVAGQSTLGWCQIPVTDIVNEFFPKDGVRQLSYQLRNRDGSRGQGVVNIEVKLTGLFHERFQRLQRPLICSGGLPQQYVPETVIGIPVNQSDGREFLPIINMHKQHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAR6</accession>
    <name>A0AAF0WAR6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0104820</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108204760"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000032">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000033">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
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        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
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        <property type="entry name" value="ATPase_P-type_domA"/>
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        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
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        <property type="entry name" value="P-type_ATPase_N"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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        <property type="entry name" value="P_typ_ATPase_c"/>
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    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF19">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
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        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E1-E2_ATPase"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
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    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="819"/>
            <end position="839"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="845"/>
            <end position="862"/>
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            <begin position="895"/>
            <end position="915"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="927"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="962"/>
            <end position="984"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="990"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="18">
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            <begin position="100"/>
            <end position="163"/>
        </location>
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    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
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            <begin position="779"/>
            <end position="1016"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1056"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1071"/>
            <end position="1094"/>
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    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
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<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WEG4</accession>
    <name>A0AAF0WEG4_DAUCS</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
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                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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        <property type="entry name" value="TRANSCRIPTION FACTOR GTE8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
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    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="122"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG89712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="624" mass="69402" checksum="2E0E7DAFECABF2F0" modified="2024-05-29" version="1">MTASDDVPKIRLKIKFSFKNADLLLKSGSSEVRKQTFMNEDCAQNISVDGNRGSTMKKSHEAAPHASDSLILYGADSSNVASPGQSSRKRGLLRVIDSAMNKRQKMDPSLKQHCGSILEALIKHPAALGFSEPVDPIKFNIPDYFSVVSSPMDLGTVRSKLQNDMYFSTEEFKDDVRLTFSNAMLYNPSDNIFNRNAKKLDGIFNTKLKSLDARLKCESMNPKQNSHSSGRERKTTETKYLCRRRSSVHSGLAPTISMSTEDKRKLTEEFVVATRPKIRENLMILNTGAANVSYSHIYKEVGINYAQISEGYLQSQEPAKNIQNSRSPGDKILQKAIGSRSTCRTAKTMPSASMVATRCSSCGSLECHCCPLDGYACAASSRDLASERHMGQNCDGSKMEHGSGLISDACKSDPESDGAVSVLDEQKICSSPQTSIVGTTGVAAEGWTTDSDLQLSPKKALRAAMLKSRFVDTILKAKQKTLLDHVEKVDPVKFRQQKEKLERQQLAEKARIEAQIRAAEVASSLRAQEELRIRRERERDEARIALQKMEKTVEIDENLNILKEFDMFSRGSDKSLVPFEASQGGHLGNILEHLGLYMKDDYMDYEDEEEGLFLHDREEGLVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKX4</accession>
    <name>A0AAF0WKX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0209324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108209976"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF55">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="202"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="313"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
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        <location>
            <begin position="392"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG90083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="519" mass="57420" checksum="6833BB4F7CDEFB31" modified="2024-05-29" version="1">MGKKHSSSKKDGDLKPPSSSSGDDIFKSIFGFVSQEQEAQASIFSDNNPFKRKPIDPSQQSLKIEQENDKNQLGSLGSSEFETPDGGDGKKRKRRKEKVGGVGEGEESEPKGFSKKVKGKGGSLGEDEGGSVGVELKVQDKKKRKRGDIEAEYEAKRYGEVVVKDEKELGGGNVGEKRKAMDSVVSMVDKEEGFDDEEKLLRTVFVGNLPLKVKKKTLLKEFSQFGEIDSVRIRSVPIMNSKTPRKGAVIKKEFNAAVDNVHAYIVFKEDESAKASLSHNMAVVGGNHIRVDRACPPRKKLKGEDVPLYDCKRTVFIGNLPFDVKDEEIYQLFAGLPNMNSSVEAIRVVRDPGTSMGKGIAYVLFKTKEAVNLVVRKRNLKIRDRDLRLSYSKPNTTPSKHTNPSPFDQKNSSAKKFGAKSTTPDSKAKTMSYQGLRASKSGVQKKVHTKKFDALKINSRSVQVQKPKERTEKRPSVAARKAKALRIASNGSKEAGGKKRKIENRTPGSSQKSKKPRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WL09</accession>
    <name>A0AAF0WL09_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0209386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WL09"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000125">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000927">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000470">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g66520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000804">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF485">
        <property type="entry name" value="REPEAT-LIKE SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="70738" checksum="D519452969199845" modified="2024-05-29" version="1">MSHISTRSLQKFIATQKSFSKTPPKLWPQLNHTHSIDHKGLPILNLTHPILRTLDTHNVSLHNFKQVQAQLIVSGLFQHSLAFSRVVKKLCSLPSGVNYAVELFDHFDEPDAFVCNSIIRCFVNLNDPLSGLGFYYEKMVGGFVRPNHYSFPIIVKVCGELGCVREGEKVHGRIVKHGFEFDLFVRNALIHMYCVCGRVWSAEKVFGLSCELDLVSWNSMIDGYVKNGEVGLARGLFDQMVERDVVSWNSMIKGYVGVGDMERAKELFDGMPCRDLVSWNCLIDGFARVGNVVAACEFFDQMAVRNVVTWNTVLALYVRVNKYGECLRLFDRMMAEGEVSPNEASIMSVLTACAHLTDLDRGKWVHSYIKHDKNIKADVLLLTALLTMYSKCGAMDLAKDVFDEMPDKSVVSWNSMIMGYGMHGDGEKALRIFMEMERSGQMPNDATFVCILSACTHAGMILEGWWYFDIMHRVYKIEPKIDHYGCIVDLLGRAGLLKDSEDVIKNMPMEAGPAFWGALLSACRSHSNVELGEVVAKRLIDLEPEDIGPYVLLSNIYAAEGRWDDVDNVRRRIVEKGFQKASGSSVFQVGDTRGESLQVSSSVHKRSMVYSMLSDMGTQIKMSSRYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLW7</accession>
    <name>A0AAF0WLW7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydroneopterin aldolase</fullName>
            <ecNumber evidence="4">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0310264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHNA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLW7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase/epimerase" evidence="8">
        <location>
            <begin position="12"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG91016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="115" mass="12841" checksum="CF6C5950C4645FB1" modified="2024-05-29" version="1">MANDGEMKGDKLVLRGLKFHGYHGVKEEEKKLGQKFVIDIDAWMDLHQIAKEVVEGTPQNLLETVAQLIASATLTNHSRISAIRVQVQKPHVSVPGHIDFLGVEITRYRSTEGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WM71</accession>
    <name>A0AAF0WM71_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0310256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WM71"/>
    <dbReference type="KEGG" id="dcr:108215225"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000554">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g41720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003312">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="541"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="576"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="611"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="751"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG91008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="826" mass="93225" checksum="0122E9FDBDAF05A0" modified="2024-05-29" version="1">MAALAQHHHQLQLFNHLFSPQSQKPIKLPLKPICAKPNTDEAFKPKKQVSVDYDKGTRQVSVHLSGLRKEHLPKYQRLRVDGDKFQKDWAISELVVKIMRMKHWEDIEGLLNRWAGRFARKNFPVLIREMTVRGSIEHSLQVFSWMKQQKNYCARNDIYNMMIRLHARHNRTDQARGLFFEMQEWRCKPDAETYNALISAHARAGQWRWAMNIMDDMLRAAIPPSRATYNNLINACGSSGNWKEALNVCKKMTDNGVGPDLVTHNIVLSAYKSGGEYAKALSYFELMKGTKIRPDTTTLNIVIHCLVKNRQYMKAIDIFTSMRDKRAECDPDVVTFTTMIHLYSVSGQVEDSRAVFDTMLAEGLKPNIVSYNTLLGAYALRGMSKEALLVFNEIKKNGLRPDIVSYTSLLNAYGRSQQPGKAAEVFEAMKKNNWKPNLVSYNALLDAYGSSGHLAEAVDLMHAMEQDGVYPNIVSISTLLAACGRCCQKVKIDSILSAAESRGIELNTVAYNSAIGSYMSVGEFDKALKLYSSMRKKVKCDSITYNLLISGCNKMSRYHEALKFLDEMVDLRIPLSKEVYSSAICAYSKQGQLAEAESLFCMMKEAGYSPDNIAFTAMLHAYSAAEDGDKAFALFQEMEMADVQLDSIACSSLMRAFNQGNQPARVLSVAEFMKKRRISFSDAVFSEMASACSILGDWRTTTLLISMMEPLINGLSVGLLNKVLYSIGKSGKIEIMMKLYYKIVASGTEISFSTYDILLKNLLAFGNWRKYIEVLQWMEDSGTKPSAAMFRNILSFAHKSVGTEYAVVIQKRIESLKRESFKEIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMN3</accession>
    <name>A0AAF0WMN3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0311395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG92136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF25">
        <property type="entry name" value="MAGNESIUM TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG92136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="344" mass="37342" checksum="36F3C6B8F8391D06" modified="2024-05-29" version="1">MYKENYLGFGLAVASSAFIGSSFIIKKKGLQRAAASGSHAGAGGYGYLLEPLWWVGMITMIVGEFANFVAYIYAPAVLVTPLGALSIIVSAILAHFLLKEKLRKMGILGCALCVIGSTIIVLHAPGEHSISSVDEIWALATQPAFLIYTALAIAVVLVLVLYCESRYGQTNMMIYIGVCSINGSLTVMSIKAIGIAIKLTFEGSSQVAHFQTWIFVMVAVSCIITQLNYLNKALDTFNTSVVSPIYYAMFTSFTILASAIMFKDWSGQNASDIISVLCGFGTVLLGVAVLHNTREPVPPPSDLYTSLSPQFSWLVHANGEIWKPTDNDELHHEFIGVIRPDYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN93</accession>
    <name>A0AAF0WN93_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0311735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN93"/>
    <dbReference type="KEGG" id="dcr:108215022"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ADF_cofilin_like"/>
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    </dbReference>
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        <property type="entry name" value="Actin-depolymerizing factor 2"/>
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    </dbReference>
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        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADF-H"/>
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        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
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        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG92466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="137" mass="15583" checksum="AB7C8DAF423F4B0B" modified="2024-05-29" version="1">MANAASGMAVEDDCKLKFLDLKAKRCYRFIVFKIDGQQVVVEKLGSPDESYDDFTNSLPADECRYAVFDFDFTTNENVQKSKIVFVAWSPDTSRVRMKMVYASSKDRFKRELDGIQVELQATDPSEMSLDIVKARAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPM1</accession>
    <name>A0AAF0WPM1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CSC1-like protein At4g35870</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0313089</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPM1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF114">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WR94</accession>
    <name>A0AAF0WR94_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">Acid phosphatase</fullName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="3">Belongs to the APS1/VSP family.</text>
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    </evidence>
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</entry>
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    <accession>A0AAF0WUP1</accession>
    <name>A0AAF0WUP1_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG94943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="181" mass="20303" checksum="981AB3BE9B553BE2" modified="2024-05-29" version="1">MQKPKAKIQPKAKMQPKAPGEWTTGLWGCFSDWGNCITTLFCPCITYGQIANIVQKGKTSVFTEARNYATIMYFTGGCCLYSCFSRSKLRHKYNLPQSPCSLPDCAVQFCCELCALCQEYRELQGRGFNMAIGKLASSYAQIPTPYICIYFSNWIANMNLNNYITVLQGNTVTVKCKAIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUU6</accession>
    <name>A0AAF0WUU6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0414619</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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            <end position="97"/>
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            <end position="130"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG95306.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="157" mass="17978" checksum="F1029DCE16438F5B" modified="2024-05-29" version="1">MLLNVPLGKLQENYQTKLMESKDHRMKATSEILKNIRILKLQGWEMRFLSKILDLRKIEAGWLKKFVYTNAVVTFVFWGTPTFVVVVTFSTCMLLGIPLESGKILSALATFRILQEPIYNLPDTILVMIQTKVSLDRIAAYPKKNEICMKPVISDLA</sequence>
</entry>
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        <lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="7">Leaf</tissue>
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    </reference>
    <comment type="similarity">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WWG6"/>
    <dbReference type="KEGG" id="dcr:108218479"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042134834" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="35"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="98"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="150"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG96819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="26513" checksum="CDA60C08016FC933" modified="2024-05-29" version="1">MEANAMLFRSLFFLISLSLLSLHISATSITLYNKCSHPVWPGIQPGAGQPILARGGFKLLPKKSYTLQLPPAWSGRLWGRHGCSFNRQGHGKCATGDCGGALFCNGMGGTPPATLAEITLGSEQDFYDVSLVDGYNLAISITPIKGSGKCTYAGCVSDLNMMCPVGLQVRSHDNRQVVACRSACAAFNSPRYCCTGTYGSPQSCKPTAYSRIFKNACPRAYSYAYDDPTSIATCTGGSYFLTFCPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY86</accession>
    <name>A0AAF0WY86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0416521</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY86"/>
    <dbReference type="KEGG" id="dcr:108216705"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000014">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF44">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG97181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="256" mass="28254" checksum="FCC827D4FCDAA9AC" modified="2024-05-29" version="1">MTKALHSFFGIFGDLIGLFLFLAPTITFKRIILNKSTEEFSGIPYLMTMLNCALSGWYGMPFVSPNNILVVTLNGTGASIEAIYVLIFIIYAPKKEKLKILGILAFVLALFSTIALISVFALHHQNRKIFCGLAAGIFSVIMYGSPLSVMRLVIRTRSVEYMPFFLSLFSFLCGTSWFIFGLLGKDLFLSVPNGVGGLLGIAQLILYAIYYKDKGKTKNSTTDKSTEMGLAGCDHKSGNDEQKLGSEYRKASQDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZ37</accession>
    <name>A0AAF0WZ37_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0417640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZ37"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37741:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG98299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9116" checksum="6C7DD7CD6FB88AB3" modified="2024-05-29" version="1">MMDASDSFPAADAEKELNPGTNTEVEVDSVSSEIAEKKAVGSSAGKKEKGKNDPMQTFKTTMIVSGVVIVVLAVALAVSKKMKENKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZK2</accession>
    <name>A0AAF0WZK2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0417212</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZK2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="11">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG97871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="672" mass="77455" checksum="C77E42752CCDE35E" modified="2024-05-29" version="1">MEVAIETPVKKPKRLTSAVWNHFERVRKADICYAVCIHCKKKLSGSSNSGTTHLRNHLIRCLKRSNFDVSQILAAKRKRKDNNSLDLPNVSYDEVKRKAEAIIPLAAYRYDNEQRKDEAVNLGSIKFDQERSRLDLARMIMLHGYPLGMVDHLGFKIFIKNLQPSFEILTNSGVEYDCMKIYEKEKQKVYDMIRNFNGRISMGVGKWVSPENEEYMCLTANFVDAEWKFQKKTLNFITLDSSHTDDTLSEVVIKCLMNWEIDRKLFSLTFDDCSINDEVARGVKDWISQSKPLLKNGELFDVRCASHVLKSLVQEAIEALEEVCCKIRESVRYVKSSQATQGKFYEIASDVGINSEKRLCLDTPMMWNSTHLMLETALEYRGAFSLLQEHDPAYYTTLSEEEWELANTVTSCIKLFVEIISVFTVNKHPTANIYFPEICDVHIQLIEWCKSPNEFICSMALKMKMEFDKYWSKCRFSLTVAAILDPRFKMKLVEYYYPQIYPSGAQNYIKQVSDGMRELFNEYSVGSASLDQGCSSLPSTSNGTRDRLRGFDKFLHESSQSHSIVSDLEKYLEEPIFPRNHDFNILNWWKVHTARYPILSMMARDILCIPFSTLTSELAFSTRGRVLPNHLISLNADTREALVCGQDWMHIESEDPNQFSSYTALPVVAEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZN5</accession>
    <name>A0AAF0WZN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase subfamily 4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0417192</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZN5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000109">
        <property type="entry name" value="Chitobiosyldiphosphodolichol beta-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000083">
        <property type="entry name" value="UDP-glycosyltransferase TURAN isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="9">
        <location>
            <begin position="34"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG97851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="477" mass="53961" checksum="A83E72E7778405BA" modified="2024-05-29" version="1">MVISGKGERRGRAALVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLEHQSIHIHKMKPWPAIPHLPKILQPLMLLFKPVFQFLMLLWFLCVKIPAPDVFIVQNPPSVPTLVAVKWASWLRRSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMANGSLCVTQAMQLELAQNWGIKATVLYDQPPEFFHPTLLEEKHKLFCRIEKNLNEPSGHRDCISNGKDEHNPNATLFTTQVGNDIKLNQNRPALIVSSTSWTPDEDFNILLEAALMYDRRVAAILNEDDSTPEDVLSREYCEGKQFLYPRLLFIITGKGPEKQKYEEKLRKLKLKRVAFRTMWLSAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCGLPVCAVSYSCIKELVKVEKNGLLFSSSSELADELMMLFKGYPDECDALKLLRKGVHETRSSVSWETEWEAKAKPLVNEVISGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7I6</accession>
    <name>A0AAF0X7I6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0522228</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH02838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31729">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR RAP2-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31729:SF2">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR RAP2-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="11">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH02838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="154" mass="17259" checksum="62EB9C865F7C13F1" modified="2024-05-29" version="1">MEGSTDCCSSNLDDKRKHSKHQENTKIYRGIRMRKWGKWVAEIREPNKRSRIWLGSYSSPVAAARAYDTAVFYLRGPSARLNFPELITDEPGDQLHDLSAASIRKKATEVGARVDALQASHHGSNDSSNVSNSGRVYHKPDLNEFPSPESSEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7I7</accession>
    <name>A0AAF0X7I7_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">Vacuolar import/degradation Vid27 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0522255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X7I7"/>
    <dbReference type="KEGG" id="dcr:108222832"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Vacuolar import/degradation Vid27-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055559">
        <property type="entry name" value="CYPRO4_DUF7135"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23581">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7135" evidence="3">
        <location>
            <begin position="35"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="258"/>
            <end position="568"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23581"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH02865.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="69793" checksum="6D4938DA9B3997A7" modified="2024-05-29" version="1">MGGTHSREDLELSDSESESETESQSSPDSQAFSDRKDPETPASVDDIDAKLKALKLKYQSGGANLQNALKLYLHVGGNTPNSKWVVSDKIASYVFDKSSRIDEEGEEEEDDEELEGKDSWWVLRVGSKIRVKVSAEMQLKTLGDQLRVDLVSKGVWAMKFFRKEDYELFLSKYEECLFENTYGVEFNDGNKVKVYGKDFLGWANPEKADDSVWEDAEESFEKSPKTVTPVRRGHDLNEEFEDVADGGSIQSLALGALDNSFLVGDVGIQVVRNYNHGISGKGVCVNYENGRGSVARSTPRKAILMKAETNMLLMSPVAEGKPHTRGLHQFDIETGRVVTEWKFEKDGTDITMRDVTNDSKGAQIDQGSTFLGLDDNRLCRWDMRDRHGMVQDLTEANTPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLYSGNSMRQAKTAFPGLGLPITHVDVTYDGKWILGTTDTYLILICTMFTDKDGRSKTGFAGRMGNRISAPRLLKLTPLDSHMAGLNNKFRGAQFSWVTEDGRQERHLVATVGKFSVIWNFQQVKDGSHECYRNQVGLKSCYCYKIVLKHDSIVDSRFMHEKYAVSDSPEAPLVVATPLKVSSFSISNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9Y6</accession>
    <name>A0AAF0X9Y6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoglucanase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0522587</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9Y6"/>
    <dbReference type="KEGG" id="dcr:108223266"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041772869" description="Endoglucanase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="10">
        <location>
            <begin position="31"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH03192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="506" mass="57012" checksum="95F6A5A35021784A" modified="2024-05-29" version="1">MEMKKQLPHCLVFTISLLLVIIWTKAQAPDYADALTKSLLYFEAQRSGRLPYNQRVTWRDHSGLTDGLEQGVDLVGGYHDAGDHVKFGLPMAFTVTMLSWGVIEYGEEIARAREYEHALEAIKWGTDYFIKAHTSPNVLWAEVGDGDTDHYCWQRPEDMTTSRQAHKIDEKNPGSDLAGETAAAMAAASIVFKETNPHYSHLLLHHAQQLFEFGDKFRGQYDSSVGVVKSYYASVSGYKDELLWAALWLYKATDNEVYLDYVINNAGNFGGIGWSISEFSWDVKYAGIQIIAAMLLPEVKHKKQKEIIEQYRSKAEFYICSCMSKNNGTNIERTPGGLLFIRQWNNMQYVSSAAFLLTVYSDFLSNTNQNLSCHGITVTPEELFALAKSQVDYILGSNPKNMSYLVGYGSRYPTKVHHRGASIVSYRVNKGFIGCTQGYDDWYSHSEPNPNVVTGALVGGPDREDNFRDDRGNYMQTEACTYNTAPLVGIFAKLNQFSDLRLIASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XAL2</accession>
    <name>A0AAF0XAL2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0622892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XAL2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="69" mass="7863" checksum="B70060F439CE8AEF" modified="2024-05-29" version="1">MSEQATLFLSSFGLLGEYSPSPRIKDKVGCMHFEAYQGNNRNFAGCQFMRPCPTIEDNTIFKTVTFYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XB55</accession>
    <name>A0AAF0XB55_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0623179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XB55"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd18312">
        <property type="entry name" value="BTB_POZ_NPY3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="31"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="213"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH03779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="69289" checksum="45C9881E30D6AAE1" modified="2024-05-29" version="1">MKYMKLGSKPDAFYAQGNCRYILHVSSDLATDVTIIVGEVKFYLHKFPLMSKSNRLQKLVSGAIDVDSNEILLPDFPGGPKNFEICAKFCYGMMVTLNAYNVVAARCGAEYLEMTEDADRGNLIFKVEVFLNSSLFRSWKDSINVLQTTSSLLPWSEELKVVGRCVDSIASKTSVDPSSINWSYTYNRKLTLSNEIVDDTTKVQDRSASVPKDWWIEDICELDISLYRRVMIAIKSKGRMDANIIGEALKTYAVRWLPDSVEALLSDVHQQRNKSLVETIICLLPSDKGIGCSCSFLLKLLKVAILIGAEDLSREDLIERISLKLDEATASDLLIPAQSTQDTKYDVDLVQCLVHRFMMNENIGQDSNIVLKNEKNTDDFVLGHGPLLNVGKLIDRYLAEIATDPKLALSCFIDLSQSIPEAARPIHDGLYWAIDIYLKEHPILTKAERKKLCSMMDVKKFTANASMHAAQNERLPLRVVVQVLFFEQVRTAAIVQNTNSSGSLEVSRSTTNTDEDLEKSEPQPMTLQKQMSQLRTTNKDFQKNGKLIKEGSKHKSAIQLLPSRSRRIFDKLWVVGKGNGMGEAKSSETSGSSQSPSSMIRGEVKESPSTARDRRHSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XD48</accession>
    <name>A0AAF0XD48_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0625256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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</entry>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
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    </reference>
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        <text evidence="11">Involved in the regulation of the G1/S transition. Increases the DNA binding activity of E2F proteins after heterodimerization.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the E2F/DP family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of cell cycle"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="16">
        <location>
            <begin position="84"/>
            <end position="167"/>
        </location>
    </feature>
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        <location>
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            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="282"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="WOH06104.1"/>
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            <dbReference type="Proteomes" id="UP000077755"/>
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37003" checksum="31814C570ECB8FA7" modified="2024-05-29" version="1">MISGSNREGASKSWGTNGSGQSVSTSGSVGSPTTRSEAVVNTPPSDKTLIRLNHIDAHADDVASQATDINKKKKRGQRAVGGDKNGRGLRQFSMKVCEKVQSKGRTTYNEVADELVAEFSDPGNGISSPDQQQYDEKNIRRRVYDALNVLMAMDIISKDKKEIQWRGLPRTSMNDIEELKCELLGLRSRVEKKAAYLQELEEQYVGLQNLIQRNEQLYSSGDTPSGGVALPFILVQTRPHATVEVEISEDMQLVHFDFNSTPFELHDDNYVLKAMKVCERQQGNEPQIPSDGGESSSVPVFQPQIAQPSSLGVQSSRPPTSPPLPGILKAARVKNAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XHT0_DAUCS</name>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0626688</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02079">
        <property type="entry name" value="P-type_ATPase_HM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000022">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000043">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF5">
        <property type="entry name" value="CADMIUM_ZINC-TRANSPORTING ATPASE HMA4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="12">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH07259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1036" mass="112366" checksum="C7A769766CD8D5AE" modified="2024-05-29" version="1">METEKRSSQSSSVNKSYLEVSGICCSSEIPLIERILKPLEGVLEVTVIVPSRTVIVLHHSLLINQLQMVNALNKAGLGASVKGRGGAGDHVNNNHNKKKKKNRWPSPFVIASGFLVLSALILQHVYHPLRYLGLLAVALAIFPIFLKAVAALRNFNLSNINILVLITVAGSIVLKDYVEAGTIVFLFTFAQWLESKAIPQTALIAETGERLNADQVQLNTILSIKPGEVIPIDGVVVQGNCDVDEKTLTGESFPVSKQTGSTVWAGTINLNGYLSVNTTALAQDCVVARMANLVEEAQKNKSKTQRIVDKCTKYYTPLIIAVALCLAIIPSAIRAHNLSEWYRLALVVLVSACPCALVLSTPVAAFCALSNAAKSGLLVKGAEHLETLAKVKIMAFDKTGTITTGEFVVADFRPLVQDDHMTLHKLLYWVASIESKSSHPMAEALVKYAKSYTVEPKPDDVEEFYNFPGEGICGKIDGKDIYVGNKKIAVRAGCATLPIIEDDEMEGKSIGYIFSGSVPAGIFSLSDVCRTGVKEAIQELKSMGIKTAMLTGDHQAAAKHAQNQIGGALELVHAELLPEDKARIIKDFQRESATAMIGDGLNDAAALATADIGISMGISGSALAMETGNIILMSNDIRKIPQAVRLARKTKQKILENIFLSIVTKTAIVVLAIMGHPLVWAAVLADAGTCLLVICNSMLLLAHEHNHGKRIFSRAAHSHKHCCSGTQTVKKHKQKRCSFRTCCVQSHSKRTGLAKGQGCCEVDDDEAKGVQHNIAISNSKSHCHQRCCSANKVETKCISHFHSPKHPCESVSPKSCGEIEPLRKSTETAGCCEYDDNKLEKVKHTKHANHGGCEESDKFKEAKHVNHHGICDIVHSHEEEEEGEEEGVDLVAKGHGCCSTSCQSEYEPKSCSPIYDTSHCHHEKSSSIISHERLEEDADDRSLSCPGAEQQAAILRANKHCHMKSCENEHNISEYQVRNMVKSCGHTDWASSPTPSSAVYSNIQVRHVRGCCKSLSKECCGKAPVFGGGLSEIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIH9</accession>
    <name>A0AAF0XIH9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0626450</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIH9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056876">
        <property type="entry name" value="Msl2-3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057483">
        <property type="entry name" value="MSL2/3_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045042">
        <property type="entry name" value="YnaI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634:SF2">
        <property type="entry name" value="LOW CONDUCTANCE MECHANOSENSITIVE CHANNEL YNAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634">
        <property type="entry name" value="OW CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24956">
        <property type="entry name" value="Msl2-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25237">
        <property type="entry name" value="MSL2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive channel protein 2/3 transmembrane" evidence="12">
        <location>
            <begin position="153"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="10">
        <location>
            <begin position="283"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel protein 2/3 C-terminal" evidence="11">
        <location>
            <begin position="358"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="497"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="571"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="636"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="672"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="689" mass="76245" checksum="8C3B26E1D8762183" modified="2024-05-29" version="1">MAASTSLLLLREVNARCTYNGRRHKSQYKGAIKKDPFHLFSITLSSHASLQDAWSIHLVNSVQRHQSPVLSRYNVFFCRSTLTPSKGHEIPLVKTIAELTRSCNAFPQGSLVLQLIPAIGVLAFAAWGLVPLVRLCLVLFQKKDITWKNSSAHYVLTSYVRPLLLWSGAALICRVLDPVILPSVASQAVKQRLLNFVQSLSAVLAFAYCFSSLIQQTQKFFTETKDFSDERDMGFEFAGKAVYTAVWVAGVSLFMELLGFSTQKWLTAGGLGTVLITLAGREIFTNFLSSVMIHASRPFVLNEWIQTKIGGYEVSGTVEHIGWWSPTIIRADDREAVHIPNHKFTVNVVRNLTQKTHWRVKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRIFLDNIDPENQALRIMVSCFVKTSRFEEYMCVKEAILLDLLRVISHHHARLATPIRTVLKTYGEAEAERSPFSDLYTRPRAAANPPFLLIEPSKINSDEKSKVSAQSDSNLDIKDGSGLNNLETDVKDASASPSSSNPHIKVNEVAPSNSEILTATPVESLSDNSKKSIKYPGDVLSSTSGDHVSSGESSSVNTSSVSQEPGRQNSPAKPLKPDVERSVSVLEDNILLGVALDGSKRTLPIDDEDISHSSITEEPSELATRRKGNGTTTLSKGNKDDPTPSRTTDQLEKD</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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            <begin position="243"/>
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        </location>
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        </location>
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        </location>
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    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="YhbY-like_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
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            <end position="282"/>
        </location>
    </feature>
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        </location>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="474" mass="53692" checksum="4072723CE86C6C27" modified="2024-05-29" version="1">MAVVAKLHLFSNLPSNPPPQPQPTPQSHRPPKPPIPIPKYPPPKPKKPPTPPNPAFKTHHNRSKYYKPVKQGVLASDDGRTILIGDSGVSYQLPGAPFEFQFSYSETPKVKPLAIREPAFLPFAPPTMNRPWTGKAPMKKAKRNVRLFGSGEEESDGVRRMEMRGGYEVKELFGGELRPRNEVLGRPLSKAEARELIKPYLSSNRQVNLGRDGLTHNMLELIHTHWRREPICKVRSLGVPTVDMNNVCRCLEEKTGGKIIFRVGGIAYLFRGRNYDPRNRPRYPVMLWKPATPVYPKLIQEAPEGLTKAEADVFREKGKKLLPICKLAKNGVYATLVRDVRSAFEECAIVKINCTGMHASDYKKIGAKLKELVPCVLLSFDDEQILMWRGHDWKSMYKRSPKAPSPVHDTTANGMNRSGMHMIMFNKGSEVARNVLDAHYNELLNCFMYLLYINFAQTILADLKKAFYMIILTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XL10</accession>
    <name>A0AAF0XL10_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
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    <gene>
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    <organism evidence="6 7">
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="6">Leaf</tissue>
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            <begin position="67"/>
            <end position="145"/>
        </location>
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            <begin position="683"/>
            <end position="780"/>
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            <begin position="179"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMH2</accession>
    <name>A0AAF0XMH2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ROOT HAIR DEFECTIVE 3 homolog</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein SEY1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0730291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein ROOT HAIR DEFECTIVE 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="701"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="725"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="37"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="759"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="761"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="774"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH10818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="811" mass="90185" checksum="C6C5AAD3875F2D09" modified="2024-05-29" version="1">MSKDGECCSTHLIEGDGTFNASGLENFIKEVKLSECGLSYAVVSIMGPQSSGKSTLLNHLFHTNFREMDAYRGRSQTTKGIWMARCAGIEPCTIVMDLEGTDGRERGEDDTAFEKQSALFALAVSDIVLINMWCHDIGREQAANKPLLKTVFQVMMRLFSPRKTTLMFVIRDKTRTPLENLEPVLREDVQKIWDSVPKPQAHKETPLSEFFNVEVVALSSYEEKEEQFREQVASLRQRFNQSIAPGGLAGDRRGVVPASGFSFSSQEIWRVIKENKDLDLPAHKVMVATVRCEEIANEKYSSFTANKDWLEVEEAVQSHPVPGFGKKLSSILDTCLSEYDAEATYFEEGVRSSKRKQLEEKLLQIVQPAYQSMLGHIRSRTLDKFKDALDDALKGGQGFAVAARYSTKAFLKFFDEESADAIIKQANWDTAKVREKVQRDIDAHIAAVRTSKLSELTTTYEKKLSEALSAPVEALLDGASDDTWPSIRKLLQRESEAAVSGFSTALSGFEMDEQDKDKMISSLEDYARGIVEGKAKEEAGRVMIRMKDRFTTLFSQDSDSMPRIWTGKEDIRAITKTARSASLKMLSVMSAIRLDDEIDNIENTLSLALVDSNSGSASNKAASLDALASSTWEKVPASKTLITPVQCKSLWRQFKTETEYTVTQAIAAQEASKRSNNWMPPPWAIVAIILLGFNEFMTLLRNPLWLLVIFVGYLLTKALWVQLDISGEFRHGALPGLLSLSTKFLPTVMNLLKKLAEEGQVPTSTNQRNPSLASKSFPSGSSDISSSASSEVISSENGTEYSSPIVHDKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPX4</accession>
    <name>A0AAF0XPX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0730267</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPX4"/>
    <dbReference type="KEGG" id="dcr:108193815"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000225">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF2">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="11">
        <location>
            <begin position="173"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH10794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="293" mass="32087" checksum="34AD0EEB55DECB60" modified="2024-05-29" version="1">MKKGLSSRGWDCEDSKLELRLAPPGDEDWEIKKECITETCENTSSAFLSLGCFNGSFQENSSKVLDDATVSPWSRSCSQYKQSLAPAVGKESIQNAEKRGFSPAPANNTAVHKSSQKRITSAPGPVVGWPPIRSFRKNIASGCASKLPTETKATNEVSTKIAGKRPTERFQKGLFVKINMDGVPIGRKVDLKAYDSYEKLSSAVDELFRGLLAAQTDPSVNNIQNSQGEKAITGLLDGTGEYTLVYEDNEGDRMLVGDVPWHMFVSTVKRLRVLKSSELSSLLFGSKQEREPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XT39</accession>
    <name>A0AAF0XT39_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0833020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XT39"/>
    <dbReference type="KEGG" id="dcr:108197512"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36402">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36402:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH13510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="198" mass="23732" checksum="BCECF26773648B54" modified="2024-05-29" version="1">MSLFLRSPSKPKTLIPLFTSLRSYAAVSPPPSQTHHTHHQKTHTYSPPSPFLSSWDPPKNPKEAQWQLAMLRRQYDKQVREVRKEYMQEMEVMRAEKARKDEAKREALRVANEERKAAKLEAKKVRAAERKLEEEEFRRMLLKEKEEKLEYWRKRENLVQEKKEKKKEDLRRQSSMWIDENKLEARTLEALVDGSNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XT98</accession>
    <name>A0AAF0XT98_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0933251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XT98"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="4">
        <location>
            <begin position="21"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH13740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="195" mass="20817" checksum="7FCADB62A11C697F" modified="2024-05-29" version="1">MTIRYIYICCSKLIGAKYYNKAYKRYTGRFNASLNTARDYEGHGTHTLSTAGGNFVSGANVSGHGNGIAKGGSPRARVAAYKVCWPPVNGSECYDSDIMKGIDTAIHDGVDVLSVSLGGKPSDYFSDGIAIGAFHAVKKGMVVVCSAGNDGPAYRTVSNLAPWIITVGASTTDRVFEAYVQLSNGQRLKVEHHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XUH2</accession>
    <name>A0AAF0XUH2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectate lyase</fullName>
            <ecNumber evidence="4 10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0832152</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XUH2"/>
    <dbReference type="KEGG" id="dcr:108198420"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007524">
        <property type="entry name" value="Pec_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF184">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04431">
        <property type="entry name" value="Pec_lyase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041768244" description="Pectate lyase" evidence="10">
        <location>
            <begin position="31"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="11">
        <location>
            <begin position="163"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH12646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="436" mass="48593" checksum="311A31D5C3758E65" modified="2024-05-29" version="1">MPILRRKKLAFFVFFFSLAIVLPCMHAGIAEVDDFLLARAKKAKENAEKSYDPHPEETTDNVNKQVGEILKRNKTRRLLGESDDCTATNPIDRCWRCRADWETDRKRLAECARGFGSRTTGGKDGEFYVVTDESDNDVENPAPGTLRHAVIQEVPLWIIFSKSMVIRLQQELIITSNKTIDGRGKTVHIAYGAGLTLQFVENVIVHGIRIHHIIVTPGGIVRDSVFHLGLRTISDGDGINIYGSSNIWVDHNSFGKCSDGQVDAIQGSTAITISNNKFNNHDHVMLLGGSDSCEADKIMQVTVAFNRFGKGLVQRMPRVRFGFVHVINNDYNRWEMYAIGGSSAPTIICQGNRFKGPDNGHAKEVTKREATPEVWKHWQWRSEGDKFTNGAYFVESGPPFSSEAVKMNLIKAKPGSFAGRLTRYAGALKCKPGHPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ADT1</accession>
    <name>A0AAF1ADT1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0100279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ADT1"/>
    <dbReference type="KEGG" id="dcr:108205329"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052733">
        <property type="entry name" value="Chloroplast_QOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013:SF1">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="17"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="330" mass="34892" checksum="14B642DCD9611BAC" modified="2024-05-29" version="1">MASANLMQAVWYDSYGGGASALKHVEAPLPSPKNDEVLIKVEASSINPIDCKVQNGLLRPLLPRRFPFIPVTDIAGEIVDLGSDVKNFKTGDKVVAKLNDVSGGGLAEYAVTKESLTVARPPQVSAAEAAALVTAGITALQTITESAGLKVDKSGPKANILVTAASGGVGHYAVQLAKLGNIHVTATCGARNFDFVKSLGADEVIDYKTPEGAAVKSPSGKKYDAVINCTTGIRWTTFEPNLSEHGKVVDMTPGAGTFANFALKKLTFSKKIVVPFLVNCKADNLEYLVKLVEQGKLKTVIDSKYPFSKAEEAWGRSLSGHAVGKIIIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJH9</accession>
    <name>A0AAF1AJH9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Berberine/berberine-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0104068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJH9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BBE"/>
    </dbReference>
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        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Berberine/berberine-like" evidence="3">
        <location>
            <begin position="60"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG84883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="124" mass="14639" checksum="7B2964535CF052E5" modified="2024-05-29" version="1">MSKISESEIPFPHRKDYMYSIQYLTAWNDANKDSSTNHINWIRDVYKYMAPYVSKSPRAAYVNYRDLDLGINKNGNTSFVEASAWGLKYFKGNFERLVRVKSEVDPDNFFRHEQSIPALPKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKN5</accession>
    <name>A0AAF1AKN5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0102909</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE V SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG83731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="176" mass="19647" checksum="C875D024E12AE783" modified="2024-05-29" version="1">MYLKAQLQWNVIIPAENLDAKGLALQKAIIVRLLDEFSAKKATSTLGYFLAVTTLDKIGEGKVRQHSGDVLFPVTFTCLTYKAFAGEVLEGEVHKILKHGVFLRCGPVEHIYLSHLKMQGYSFVPGENPIFMSEKSAKIEKGVKLRVMVIGVRYMEAEREFQAVANLDGDYLGPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALV8</accession>
    <name>A0AAF1ALV8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0104480</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALV8"/>
    <dbReference type="KEGG" id="dcr:108194862"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000467">
        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF679">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 10 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042206269" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="30"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="29"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="138"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="358"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG85292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="674" mass="75667" checksum="DC19923038311F25" modified="2024-05-29" version="1">MAFVINFSYITPLVFFILSLVFLHDIVHADPPYKLCQGENFTENSAFQINRDDLLGSLSLNAASLPKFYNTSSGNDKNTVYGLFLCYNYVKHDECTKCAEVATKDIRNLCKTSKEALLWSENCQLRYSDQKFFGLLDVADNVKQTNPKPFSDPGRLQSILNPVLLSMSKLVASSIDMVATKNISFPGDNPIYAFAQCTKDLSSEDCNTCLRAAISNISSCCYSYRGARLFSRSCFLRYEFNNFIDLDDGGITVPEIPKHSDGRNKQMIVALIFGAMAVVIAVISFYLYCLAPRKQASNSNAHNIGKGRFFHQQVQLPEANEHSDTYAMLQKFQALNNLDPQEFPFFPMESVLVATNDFSDSNKLGQGGFGPVYKGTLPTGEKIAVKRLSATSTQGSDEFVNEVLLIFKLQHKNLVRLLGFCIDKEERILVYEYMSNSSLDLILNDESKRAQVSWTVRLDIVKGIARGMLYLHQDSRLRIIHRDLKPSNVLLDKEMNPKISDFGMARIFGGSDCQANSTAKIVGTYGYIAPEFAMEGLYSIKSDVFSFGVLLLEIISGKRNAEFHISTRTKSLLSYAWELWNEGKGKDLMDPLLADACSLDDFLRCMHIGLLCVQEDSYDRPTMLSVVMMLNCETTALTQPERPAYSVGRLPHHQKTCYNNLSINDLSISNAYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANJ5</accession>
    <name>A0AAF1ANJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0208530</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasmic vesicle</location>
            <location evidence="1 14">COPI-coated vesicle membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Golgi apparatus membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="14">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANJ5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000008">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF68">
        <property type="entry name" value="COATOMER SUBUNIT BETA'-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="225"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="319"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="595"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15546" checksum="FB0918F9AF6815F5" modified="2024-05-29" version="1">MSTGKETAGEGLATGIHNIMIEDHVKVTCFSEVSDDLTLHFQIIRLPNQIYAWIGCNSAKFGHLYAAAPTRPNNTVSVASLLAGASDNTGSGIARRIVLKTGLNVILACNIPKENPMLEVNAEKRLVQKLIELGYAKPKSKGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF7">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="105"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="28933" checksum="1923D7D2A44CE809" modified="2024-05-29" version="1">MKNEVENSPQSSSASGQKSTSSSSSFPLSFREIAGACGVVLGLLGGLLCVYFTMPVSDYSFLKLPKTLEDLQILRDHLESYTNDYTIQVLVGYCMVYIFMQTFMIPGTVFMSLLAGSLFGVYKGVALVVFTATAGASSCYFLSKLIGRPLVFFLWPDKLNFFQSQVAKRRNGLLNYMLFLRVTPTLPNTFINVASPIVDVPFHIFFFATSVGLIPAAFVTVRAGIALGDLHSMGDLYDFQSMATLFLIGIVSVTPTLMSNKDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B1E3</accession>
    <name>A0AAF1B1E3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0522364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B1E3"/>
    <dbReference type="KEGG" id="dcr:108222792"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF7">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH02974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="54148" checksum="6C7291B4BF07A1EC" modified="2024-05-29" version="1">MKSKISSNGSNGLFQKISTFRDPKASIVPVLDQYIREGNKVKAADLQRFVRELRSRKRYLHALQLSEWVNTNNYCRDSSGNHAVQLDLIGAVRGIDAAENYFSNLTDKEKDERTYGALLNCYTREGVVDKSLSLMQTMKEIGFVTSPLAYNNLMCLYSRTDQPEKVLDLLSEMKKNGIPPNNFSYRICINTCGDQLDFNSMEKLLEEMESQPQITMDWSTYSTAVNHYINGDHKDKALVVLKKLEKINKDAIGYSHLISHYAKLGSKDQVMRLWGLQKVICKKQVNRDYITMLGMLVRLGEFEECTKVLKEWESSCRTFDFRVPNVLFIGLCSKGLTEKAETLLKNMIGTGKTTIPNSWAIISFGYLQMINLEKAFECMKEALAVSQESPGWKPKPVLISSLLQWLGEEGKITDVEAFVSSLRSVIPVNREMYHALIKANIRGGEGVDEIIERMKSDKIDADEDTMRILNSQTDGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B366</accession>
    <name>A0AAF1B366_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0522323</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B366"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF48">
        <property type="entry name" value="PUTATIVE THIOL PEPTIDASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="17"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="222" mass="24958" checksum="D5E6F261D41EC07E" modified="2024-05-29" version="1">MCLKGKVKLTSHCSRSTPVYLNVYDLTPMNGYAYWAGFGIFHSGVEVHGVEYAFGAHDYPTSGVFEVQPRECPGFSFRKTIFIGTTCLSPDQVREFTEHHSASYNGDTYHLIAKNCNHFCEDICYKLTGKHIPKWVNRLAKIGSIFNCVLPEALRISPVQHDPDYQVYDSDKKRLRSNFSNCLTSVSTRQKQLSSSSLFILSPSKGCLPPSDLSRSVENQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B404</accession>
    <name>A0AAF1B404_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0522581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + H2O = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:79699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:79700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:56312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140372"/>
            <dbReference type="ChEBI" id="CHEBI:140374"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:56313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>6-diphospho-1D-myo-inositol pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:79703"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:230534"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:79704"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Atypical dual-specificity phosphatase Siw14-like subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="1"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B404"/>
    <dbReference type="KEGG" id="dcr:108223431"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052847">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14528">
        <property type="entry name" value="PFA-DSP_Siw14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000024">
        <property type="entry name" value="probable tyrosine-protein phosphatase At1g05000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF48">
        <property type="entry name" value="INOSITOL PHOSPHATASE SIW14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="45"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH03187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="202" mass="22880" checksum="FAFBD249E7F8D980" modified="2024-05-29" version="1">MKAVSVTDGDGNGDTCRTIGVALVSGSSPTGEAYEYDEQYTPPFNFAMVENGIYRSGFPDSTNFSFLQTLSLRSIVYLCPEPYPEVNAEFLRSNGIRLFQFGVDGSKEPFVNIPEDTIREALKVVIDIRNHPLLIHCKRGKHRTGCVVGCLRKLQKWCLTSIFDEYQRFAAAKARVSDQRFMELFDISTFKHIPKEFSCLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B5G2</accession>
    <name>A0AAF1B5G2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0624764</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B5G2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010100">
        <property type="term" value="P:negative regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000610">
        <property type="entry name" value="B-box zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051979">
        <property type="entry name" value="B-box_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832:SF5">
        <property type="entry name" value="OS09G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="12">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH05348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="24925" checksum="43D4A2FF2BD21DE5" modified="2024-05-29" version="1">MRTLCDACESAAALVFCAADEASLCRSCDEKVHMCNKLASRHVRVGLADPIAVPRCDICENEPAFFYCETDASSLCLQCDMVIHVGSKRNHARYLFFRQRVEFPGDKASQAEDLTLKLEGESKDKTGQNQSSKPVSAENQDNCSISKARTMNASTQGLANRGTMFDLNVKPQKNNGEGSNHQLPLGSFCYHVIGIVKKKKTFTHYFSKFSFSNKNITSIFLFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCJ3</accession>
    <name>A0AAF1BCJ3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0933879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCJ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH14360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="35" mass="3986" checksum="71710DDF5A3A78CF" modified="2024-05-29" version="1">MVGCSRPSCTYRFFFGVNICNAVHPTINLIRIIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEB6</accession>
    <name>A0AAF1BEB6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH09192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="794" mass="87740" checksum="D4BBDFD7CB0EE499" modified="2016-07-06" version="1">MSSVDHADFKKQADKVRRYDELCTYDVPLNSPGLYATGFQEFLTYVLPRLELQLVLMFSLTQALHLLFKRFNFPRNFSEILTGIIIGKTLLGHIVGFEKHQLLFPDDDILLETLMKLGFIFYMFLVGVKMDPNLVPKAGKKGWSIGVSAAVCPFIVSSILGSQKLLDYYLPIYRRPAIRAIVKIIGLSPFPVIVALLIDLKIMNSELGRLALAAGLICDVLNLIQILGSTIYRIYFSEYGIPVVILTLIEALLVTIASISSHVLFLKIIQLTPEGKPVKNYYISLICSCVLVSSIACNNVGLQYHFAPFVIGLAVPSGPPLGSAIADAMDTFISGLLAPFILTYCAIKMDLVVFYDLSFLNSVLFVLSVVTASKIVGVCGVAMVIGVPIRDAVNLSIIMATQGIVQGALYESVYKLQSIDRESFTVLILSILVMALVAHLSVGFLNDYSRAYSGYQKRDIQHTPFNAELRILACADRLDDAMAVIRLLETSNPSKESPLAIYVLHLVELVGRATPVVINHRLGQKNANSHSRSHQLIGLFEKFGSQRTGFVSVQGFTAMSLPQFMHQDICSLAFDKLASLIILPFHKKWSQQGKIIFDSSLRRTINRQVLEMAPCSVGILIDRRKIRPPEASQDKEAGSVYHLGVIFLGGADDREAVAYAKRIMVKAVGCCLTVVRIVASDAVQENQWNTILDTETLREVKLQGANQHNIQYREVKSKDGPETALLINTMVEDGEFNMLMVGRRHSASSPLLTGLSEWCDLPELGSVGDILASAEIIRPVSVLVMQQQIVGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XSR2</accession>
    <name>A0A161XSR2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_017958</name>
        <name evidence="6" type="ORF">DCAR_0520569</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XSR2"/>
    <dbReference type="Gramene" id="KZM94716">
        <property type="protein sequence ID" value="KZM94716"/>
        <property type="gene ID" value="DCAR_017958"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222063"/>
    <dbReference type="OMA" id="YWGYGAN"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007829241" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="58"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZM94716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH01188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="198" mass="22836" checksum="35607FAD93C10B41" modified="2016-07-06" version="1">MNPSFILLIILCLSTHSSNNTVLSVNTSSTPPPKITPLRSDNNTIYKISQQLCWNCVAEQFQFLFAHNIVRAAKWEWPLTYDTQLEDYAKWWAGQRQADCELIHSFPEFEFKLGENIFWGSGSDWKPTQAVQAWAEEEKYYNYAANSCAEEQECGHYTQIVWKTTRRVGCARVVCESGDVFMTCNYDPVGNYVGERPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZR66</accession>
    <name>A0A161ZR66_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_026085</name>
        <name evidence="5" type="ORF">DCAR_0729965</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM89010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZR66"/>
    <dbReference type="Gramene" id="KZM89010">
        <property type="protein sequence ID" value="KZM89010"/>
        <property type="gene ID" value="DCAR_026085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="302"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="683"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="692"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM89010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH10496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="847" mass="96755" checksum="A8221F0AE814E7FA" modified="2016-07-06" version="1">MKREKQRGKKEFQISNWNPDFQWISEINQEDPSGQDELLRWIGKYGFLLEDDMLKALGKKNMKLLNPAVARINYLSTKIERSSLLAASRGEEEVIIDIQTFLMNEGWWDRAQRIKLRDFGLDIGVSQTRLNEDENMTRAFIQSYKELVHPSVLNMVEKRDPEGINLALNHIHSGSLNKSRRARAQRTAVKDFFSNIDDGDTKLGKEDRQRKSLDSKEALMRFIFSHSDKVDPLTLQGVSYNKDKDISEALRQIHSNSLRISIEEKMKCKSSYLEAAKSNRTTAVPKVIPLSNKKSPVPLRWDSLFFTGFEGVSVGDIWKGIKQVARIKDIVIPKRLDKYNKKFGFIKPSSVEDANRLLQVSNQLVLGGRCVRIERARDRHPQLLKKSSVPLEGKETKNDEMKEGNEGKESGKENIQVVLEEGMEEWVDMLARSVRIDLEVDYAPDSLWELILSKGFGDLGVRKRGPYSFLISFPDSLSLEEMDWKALEIDIKKVSKAALEDLCLSKLLSQWGVLASSSFPNIFQNELSNPRVCLVTPSMKEIHGNLNVHISGRDFTVEVSEIRNVVFWKDEVPYKKNNADSIFSSKSSQARTVEESVKPLLHPEVMSVGVLSDKSDTKSFGSSVELLQDSCDDSINYIEPVSEELRYRQARTENWNRAVESSTSFFPGQEDVRKECLNESSGNSINTKKWNRRGECESHYNSDKDSLSHDVLNADTSSNNSLTHSNKSFPELCSKLQNLRIKSKGGRMRKGRKFGCFDKNRRQNRKREAVPDWMIGRYVRVWNSAKTRKARGKMIEVRKKRAKETEQGKKGEMGNISAEDIYHLGVTLGLKPTKEKGQMIDLIEGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZSZ1</accession>
    <name>A0A161ZSZ1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_021763</name>
        <name evidence="5" type="ORF">DCAR_0624442</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="4">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="114"/>
        </location>
    </feature>
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            <end position="109"/>
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        <source>
            <dbReference type="EMBL" id="KZM94032.1"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH00399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="293" mass="32103" checksum="951D90651A15645C" modified="2016-07-06" version="1">MFTSSYHVYLLGYKPKSVRKRKNFAEKENVSGNSSKRFSFPLTPLTPNSLGYKPKSVRKRKNFAEKENVSGNSSKRFSFPLTPLTPNSLGESQTANATPETLNSRTSTLEDGGLGKRTIFNRTVQNQIATPTTPLSNITNVLGSPQLANRSTIKTSILEDGGLANRTIFDRTGQNQISTPTTPLSNITNVLGNPLLTNRSTANLHLGNGTQESRKKLFSSISSHNGKLNVGANLTRTGNAVKKRFEETTKTLFPEKASQNEEKTRYLQDDDIECSIATDCVLSEDSDSEFGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AHI2</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name evidence="3" type="ORF">DCAR_009725</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
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    <name>A0A162AIA6_DAUCS</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN01463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="WOG92285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A162AIA6"/>
    <dbReference type="Gramene" id="KZN01463">
        <property type="protein sequence ID" value="KZN01463"/>
        <property type="gene ID" value="DCAR_010217"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210468"/>
    <dbReference type="OMA" id="DFQLCAW"/>
    <dbReference type="OrthoDB" id="67850at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN01463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG92285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60177" checksum="21BF4FC4EAA1E7C2" modified="2016-07-06" version="1">MSTLSNYIIGEQGFLKHRFLGFLIWQTITSLSLYSLSKLTLFSFFTPAPKLTPSLLSLFLLLTFLLSNLLFSTALHLICSPHQPLSFASPVEMLFGVFRLLFLSGAGPVGSEGRRRVGASLGFVGFVFFCGVSGAVAVGSVCWSCMEFGGGFREVGFKGFVVGLVYGLVFVFRKRWIIEFPIVQRPLFFSFKMGIPSSLRRALKLSCTGYLISTFLAFFLPNEFKSMVTLGKFINEQILLFIGIFLVLICWELCLHLHQVLHTKRYSFAPPKGSAAAETNPSEPLLAALEESHAKTLLKYLAYLDLYMVCEGNANTWRRAAFFEESGETYKRVIAVCLKPLEQLAVELGECVETDSADAFKVSSQLKSPTERVAESRLEQAFYNIQLCAWSAGITASLTVHSRKEDRFGVAQLTESNAAVVSTLLSCLLAVELCMGKKTSVHSAQYLTGPAGIKWATLSNGKRDPAVSTVGKRKGSPLYSKAYVMADILRTSIYRIVSAFHKEMLDSTKSGLHDKDWIISSKPCYGSRELLLQKLNLFLNFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AKK5</accession>
    <name>A0A162AKK5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0103122</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_002972"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220306"/>
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    <dbReference type="OrthoDB" id="10264956at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="GT29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044782">
        <property type="entry name" value="SIA1/STLP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47486">
        <property type="entry name" value="SIALYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47486:SF1">
        <property type="entry name" value="SIALYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG83943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="470" mass="53245" checksum="D2CE54FFD8F21D2C" modified="2016-07-06" version="1">MKRPPHKASRSSILYLISAAAIFTLFFVAVQFTFFTGKNKIPSEDARILFDFQSNVQQCIANRGLGLTANIIDHCKLVLKFPEGTNSTWYNAQFKVFEPLEYSYDVCEAILLWEQYRNMTTVLTREYLDARPDGWLEYAAKRIAQLGAEKCYNRSLCEEHLNLILPGKPPFRPRQFKTCAVVGNSGDLLKTEFGKEIDSHDAVIRDNEAPVNDKYAKHVGLKRDFRLVVRGAARNMVKILDGSTDEVLIIKSVTHRDFNAMIKSIPNPVYLFQGIVLRRGAKGTGMKSIELALSMCEIVDIYGFTVDPGYTEWTRYFSTPRKGHNPLQGRAYYQLLECLGVIRIHSPMRAERMQDWSDVPSREMIGRAHAAALRLKRSQVGQVDGLGQFGSCKVWGNADSNGNGRISGSPDMSPVRKNSNYSKWEVMPFTSLRREAQDHYVQMEGVSLYKMDGNKLDDLVCVRHSLISEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AX10</accession>
    <name>A0A162AX10_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0208909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="7">BZIP domain-containing protein</fullName>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Leaf</tissue>
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        <property type="entry name" value="OS03G0770000 PROTEIN"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164V678</accession>
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        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="ORF">DCAR_022769</name>
        <name evidence="12" type="ORF">DCAR_0623276</name>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="High affinity nitrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nitrate transporter 2.1"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="61"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="487"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM89868.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="527" mass="56945" checksum="8E81A04ADFDD7ED4" modified="2016-07-06" version="1">MGDTPGSSMHGVTGREPVLSFAVASPMVPTDTTAKFDLPVDSEHKAKVFKLFSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKGDVGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFVIMLTAPTVFCMSFVSDASGYIAVRFMIGFSLATFVTCQYWMSTMFNGKIIGTVNGTAAGWGNMGGGATQLIMPVLYSVIQNVGATPFTAWRIAFFIPGWLHVIMGILVLTLGQDLPDGNLGALQKTGNVSKDKFSKVFWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFDLKLQTAGIIAATFGMANLVARPFGGYASDMAARRFGMRGRLWTLWILQTLGGIFCILLGRANSLPIAVTMMIIFSVGAQAACGATFGIIPFISRRSLGIISGMTGAGGNFGSGLTQLLFFTSSSISTATGLTYMGIMIVACTMPVTLVHFPQWGSMFLPPSRDIVKGSEENYYVSEWTEEEKQKGMHQGSVKFAENSRSERGRRVASAPTPPNATPDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WDL4</accession>
    <name>A0A164WDL4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_020997</name>
        <name evidence="3" type="ORF">DCAR_0625335</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05912.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="KZM91638"/>
        <property type="gene ID" value="DCAR_020997"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF5">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM91638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="138" mass="15283" checksum="26F358B4E1DFFA31" modified="2016-07-06" version="1">MATSRPALRKAVFVKVDALKPGTQGHNVVVKVVSSEEVLKKGQSKIPSLRKTRVSECLVGDETASILFTARNDQVDVMKPGTSVIIRNAKIDMYKGNMRLAVDKWGRLEVTEPVKFKVKTDNNLSSIEYELVDVVNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YCC4</accession>
    <name>A0A164YCC4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_017541</name>
        <name evidence="3" type="ORF">DCAR_0520111</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM94298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YCC4"/>
    <dbReference type="STRING" id="79200.A0A164YCC4"/>
    <dbReference type="Gramene" id="KZM94298">
        <property type="protein sequence ID" value="KZM94298"/>
        <property type="gene ID" value="DCAR_017541"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="7"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM94298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH00737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="272" mass="31323" checksum="C0A47512F7228A68" modified="2016-07-06" version="1">MDSLLTLEPNARQDWKVRVRVSRKWRHIRLNGHTAGVNMIFVDEYDKRIHAWMNSSIMLRLEPTMVEGDVFDIENFIVRRYRAHERNQCFHDDKRIFLTNSTVVTRCNGPYQFIPRHVFDCVPLSTVGHHATQDTYLIDVCGIVMEVEPIQHFSNTIGEEQFFVRFVLADNNNNTIKAIMWNELALSVHMTMALTSQHPLIAIISSCKALIWQGGIPIVANMQATRIFMNSSHPEAVTLGVDRSLNECSELLSKYAVITVLSKVRFAAWLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZGG0</accession>
    <name>A0A164ZGG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0521893</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZGG0"/>
    <dbReference type="SMR" id="A0A164ZGG0"/>
    <dbReference type="Gramene" id="KZM95891">
        <property type="protein sequence ID" value="KZM95891"/>
        <property type="gene ID" value="DCAR_019133"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108222822"/>
    <dbReference type="OMA" id="FREWRIE"/>
    <dbReference type="OrthoDB" id="1846924at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH02504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="447" mass="49312" checksum="0A31635198A08A08" modified="2016-07-06" version="1">MGKEKIHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKSRFEEIVKEVSSYLKKVGYNPDKIAFIPISGFEGDNMIDRSTNLDWYKGPTLLEALDQISEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEIQTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFMSYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTGAKVTKAAIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165A906</accession>
    <name>A0A165A906_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_015514</name>
        <name evidence="4" type="ORF">DCAR_0415234</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165A906"/>
    <dbReference type="STRING" id="79200.A0A165A906"/>
    <dbReference type="Gramene" id="KZM97124">
        <property type="protein sequence ID" value="KZM97124"/>
        <property type="gene ID" value="DCAR_015514"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215822"/>
    <dbReference type="OMA" id="KILWEVT"/>
    <dbReference type="OrthoDB" id="2329734at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM97124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG95905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="25452" checksum="58AC1173D993ED70" modified="2016-07-06" version="1">MEKVRSMLKPKVEPQKQLREWQRRLRRECLNVDRQIRDIQREEKNVQKAIKEAAKRNDMGSAKSLAKEIIRSRKTVNRLYENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKIVNNLMKAPEVAVTMQEFSKEMTKAGVIEEMVNDSLDSALDSEDIEEETEEEIDKVLTAIAGETAAQLPEAARRERLKQPAQSVEDAEEEGADDEEELEAIRARLAKVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZWI0</accession>
    <name>A0A165ZWI0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diaminopimelate epimerase</fullName>
            <ecNumber evidence="4">5.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0310467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2S,6S)-2,6-diaminopimelate = meso-2,6-diaminopimelate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZWI0"/>
    <dbReference type="Gramene" id="KZN00504">
        <property type="protein sequence ID" value="KZN00504"/>
        <property type="gene ID" value="DCAR_009258"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108210796"/>
    <dbReference type="OMA" id="GIRCFAR"/>
    <dbReference type="OrthoDB" id="4768at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.10:FF:000009">
        <property type="entry name" value="Diaminopimelate epimerase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.10:FF:000011">
        <property type="entry name" value="Diaminopimelate epimerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689:SF0">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG91219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="366" mass="39768" checksum="2909211F9443DB03" modified="2016-07-06" version="1">MATAAATVSAQLAYSARRNTSLSSLSPSSPTFAFKFNSLRLIHTTPTNPNPSFRISMSSSIQSPPETISQLSFLDRRESGSLHFVKYHGLGNDFILVDNRDCVEPKISPEQAAKLCDRNFGIGADGVIFVLPGFNDTDYTMRIYNSDGSEPEMCGNGVRCFAKFIADLENLQGKQSFNVHTGAGLIVPEIQEDGKVKVDMGVPILNASDVPTKLPTTNNQPVVSSQLDVDGVSWNVTCVSMGNPHCVTFSNEGSQNLLVDELNLADIGPKFEHHQMFPARINTEFVQVYSRSHVKMRVWERGAGATLACGTGACAVVVAAVLEGRTERCCTVDLPGGPLEIEWREEDNHVYMTGPAEVVFYGSVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CC17</accession>
    <name>A0A166CC17_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0313962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPR1-interactor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CC17"/>
    <dbReference type="Gramene" id="KZN03564">
        <property type="protein sequence ID" value="KZN03564"/>
        <property type="gene ID" value="DCAR_012320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010112">
        <property type="term" value="P:regulation of systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031425">
        <property type="entry name" value="NPR1/NH1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33669:SF14">
        <property type="entry name" value="NRR REPRESSOR HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33669">
        <property type="entry name" value="PROTEIN NEGATIVE REGULATOR OF RESISTANCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15699">
        <property type="entry name" value="NPR1_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG94665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="120" mass="13917" checksum="BBDE61031EF12FFB" modified="2016-07-06" version="1">METRSHKNRGKALCDRDQGDDEENMEKFFELVKNIRGARHYILNYNSSDEVKLENVEGDKRRKIEEEATWKPTFQPEDFQAYRSSSTSMSQLSAFESIGGMKKDEKKRRSGLGLDLNLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166E1D3</accession>
    <name>A0A166E1D3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_006791</name>
        <name evidence="12" type="ORF">DCAR_0207647</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166E1D3"/>
    <dbReference type="Gramene" id="KZN05954">
        <property type="protein sequence ID" value="KZN05954"/>
        <property type="gene ID" value="DCAR_006791"/>
    </dbReference>
    <dbReference type="OMA" id="ASCGMRI"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000711">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g12460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000725">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000450">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF13">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872516" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="590"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN05954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG88412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="876" mass="95934" checksum="FF2F7E90665188BA" modified="2016-07-06" version="1">MKRLRKYVLCIFVLLGYQFAAVCPLTEKEILLQFKGNILSDPYKSLSSWDSSKSLCQDFNGVSCNSAGNVYKILLWNTSLGGILSPALSGLKSLRILSFFGNKFSGNIPTEYGEIDTLWKMNFSSNSLSGDIPDFIGKLSNIRYLDLSRNGFSGEIPSALFTNCFNNKFISLSRNNLSGSIPVTIGKCFNLLGVDFSFNNLSGGLPPQICDISGLVYFSVSNNGLAGPVEEQISRCKSLEFLDLASNRFTGSAPFDVLQFSNLTYFNVSSNEFVGMIPKVESCSQRLEILGASSNELSAEIPGSIAKCSGLKVLDLGYNMLNGSISVNILDLKRLLVIRLGSNLISGTIPTEFGSIELLQVLDLHNLSLVGEIPDEISNCRFLLELDVSGNALQGNIPSKLYNMSYLVILDLHQNSLNGSIPSVLGNMSNLQLIDLSENLLSGQIPLLWNLLNLTHFNLSYNNLSGVIPSSSTIQQFGASAFSHNPGLCGAPLTSCSRNDTIPTTGKSKLLSVSAIVAIVAAAVILIGVGMVTIFNRRARRWRSDEVTVVEESTPMGSADSDVIVGKLVLFSKTLPSKYADWEAGTKALLDKESLIGGGSIGTVYKTKFEGGILIAVKKLETLGRIRNQDEFEQEIGRLGNLRHPNLVSFQGYYWSSRMQLVLSDFISNGNLYDNLYGLNHPSTSTSIGNHVLNWSRRYHIALGIAKALAYLHHDCKPPILHLNIKSANILLDENFKAKLSDFGLVKFLPLLNNGGITKFHNAVGYIAPELAQSWKLSEKCDVYSFGVVVLELVTSKKPVEYHQNEVVILCEYVRRLIESGNVSDCLDRSLHGFVENELIQVMKLGLFCTSEMPSRRPSMSEAVQILESVRLGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166EVX0</accession>
    <name>A0A166EVX0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_007875</name>
        <name evidence="2" type="ORF">DCAR_0208904</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166EVX0"/>
    <dbReference type="Gramene" id="KZN07038">
        <property type="protein sequence ID" value="KZN07038"/>
        <property type="gene ID" value="DCAR_007875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN07038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG89666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="69" mass="7873" checksum="14AD2B89937852C0" modified="2016-07-06" version="1">MATTTARLAPMMFSSKVLRPSIFQPKVELPQSSTRTPFSAVAPPCRHDQLPQPFFRIQPQKNLETIFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F5R0</accession>
    <name>A0A166F5R0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_008211</name>
        <name evidence="3" type="ORF">DCAR_0209286</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166F5R0"/>
    <dbReference type="Gramene" id="KZN07374">
        <property type="protein sequence ID" value="KZN07374"/>
        <property type="gene ID" value="DCAR_008211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007873105" evidence="1">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN07374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG90045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="172" mass="17385" checksum="09CF53EFDB5D0706" modified="2016-07-06" version="1">MTYNYNSAKLVIVVSTLVFLFGSLPQGHAQVQPQLVTGLTGLLNITQIQFTTVQLACTPTGNPPSTGGGVAGVAGALLSGTCNGASGSLGRVFTNASGFAQGILTLAEGIVIDPSRGMPCFINTRLPVTGTTCTVLPPTGLLEAVFQLVSVVTSPLGGLLAVATTGPWVIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GUX6</accession>
    <name>A0A166GUX6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0102074</name>
        <name evidence="6" type="ORF">DCAR_0102078</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GUX6"/>
    <dbReference type="Gramene" id="KZN09378">
        <property type="protein sequence ID" value="KZN09378"/>
        <property type="gene ID" value="DCAR_002034"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108227747"/>
    <dbReference type="KEGG" id="dcr:108227753"/>
    <dbReference type="OMA" id="HRVSCLS"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="4">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG82904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG82908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="112" mass="12312" checksum="A9E4C055D5A7118F" modified="2016-07-06" version="1">MVAGKKTKKTHESINNRLALVMKSGKYTLGYKTVLESLRSSKGKLIIISNNCPPLRKSEIEYYAMLAKVGVHHYNGNNVDLGTACGKYFRVSCLSIVDPGDSDIIKTLPGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H465</accession>
    <name>A0A166H465_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0102442</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H465"/>
    <dbReference type="Gramene" id="KZN09698">
        <property type="protein sequence ID" value="KZN09698"/>
        <property type="gene ID" value="DCAR_002354"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226394"/>
    <dbReference type="OMA" id="TEREGCK"/>
    <dbReference type="OrthoDB" id="1926344at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF227">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="194" mass="21804" checksum="F49CB5BA2892F652" modified="2016-07-06" version="1">MESINIQEEGLNLKATELRLGLPGRDETEKETIFSFKNNKRAAPKSTEDNESEANSDAKHGDHEAAPAAKAQIVGWPPIRSYRKNNFPPKKAEAEFGIYVKVSMDGAPYLRKIDLKVYNGYADLLRALESMFKLSIGYYSEREGYKGSEYAPTYEDKDGDWMLVGDVPWDMFMSSCKRLRIMKGSEARGLGCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I237</accession>
    <name>A0A166I237_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0103525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166I237"/>
    <dbReference type="Gramene" id="KZN10664">
        <property type="protein sequence ID" value="KZN10664"/>
        <property type="gene ID" value="DCAR_003320"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212320"/>
    <dbReference type="OMA" id="LYMINIA"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG84342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="536" mass="60161" checksum="931421DF2628915A" modified="2016-07-06" version="1">MATSSILCSFILLIVFSSFSLQTSASFSRQTSGDSAQRFVQCLTRYAKNSESITQVVFTPANASYNPILQLNLQNLRFNTSGTRKPLAIVTPVEDTQVQAVIYCARKNSMNVRTRGGGHDFEGVSYTADVPFVLLDMINYNRVNIDLKTSTAWVQSGISLGEFYYKISQTSDVLAFPAGLVSTVGLTGLLGGGGYGMMKRKYALAADNTLDARIVDYNGKILDRKSMGEDLFWAIRGGDPASFCVILELKLQLVPVPKLVTYFAVQRTLEQNGSALFQKWQSTAVNVFPRDLDVRVVVDTITSNSSAREDKKTVRFIFQSLYLGKIDTLLPIMKEYFPELGLVREDCVETSWIKTAPMFSFFPVGTDPKILLNKTAPTRNPVKIKSSFTTQPISLEGLNGIWDLWLKQPVQTTLIQYTPFGGKMNEFAESALPFPHRPGVLYMINIAVTLNQNAEATLQWINDLFKYYTPYVTKNPRTSYVNYRDADLGTGSKTYQEASIWGRKYYKNNFDRLVKIKSVVDPQNFFNHKQSIPLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I549</accession>
    <name>A0A166I549_DAUCS</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0103622</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166I549"/>
    <dbReference type="Gramene" id="KZN10760">
        <property type="protein sequence ID" value="KZN10760"/>
        <property type="gene ID" value="DCAR_003416"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108200027"/>
    <dbReference type="OMA" id="NYSMETK"/>
    <dbReference type="OrthoDB" id="1937095at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF39">
        <property type="entry name" value="TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG84439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="166" mass="19480" checksum="557270054F716CD7" modified="2016-07-06" version="1">MQSPNSKHNVHKPVKNNSQSAVTQTSTPRMGKSATPQQTNKNYFGVVQKHVTKENTKPVDFRLHTQQRAVSRAIYNYTVATKVYLLERQKKQIEKVQKMIEEEEVKMLRKEMVPKAQLMPRFNKPFYPQRSTRPLTIPKEPSMMSDKCSSRFSFYNLFSLRYNEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166I830</accession>
    <name>A0A166I830_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0103711</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166I830"/>
    <dbReference type="Gramene" id="KZN10846">
        <property type="protein sequence ID" value="KZN10846"/>
        <property type="gene ID" value="DCAR_003502"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108223230"/>
    <dbReference type="OMA" id="PNMEFKE"/>
    <dbReference type="OrthoDB" id="687396at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503:SF2">
        <property type="entry name" value="OS04G0455700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503">
        <property type="entry name" value="OSJNBA0006M15.15 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG84527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="21295" checksum="F825936E8BB81999" modified="2016-07-06" version="1">MDKFQDQAALSCELSIKKARNIEFNSTGSLFVRCYMSAGNKNRVRFETREVSSSNMAWNQSFSLDCFGTKECMSSMLSEGTVSFELRWRSRISIFGRRKSQLLGKSEVPWRTVYESSTMDTEKWIVMNSRKSLADGVKPPAVQIGMKVGGALPAISKAIRQTKRCGEKCECKDCVNCDLFAIDAALEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J4J4</accession>
    <name>A0A166J4J4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="12">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_004443</name>
        <name evidence="15" type="ORF">DCAR_0104806</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="12"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J4J4"/>
    <dbReference type="STRING" id="79200.A0A166J4J4"/>
    <dbReference type="Gramene" id="KZN11787">
        <property type="protein sequence ID" value="KZN11787"/>
        <property type="gene ID" value="DCAR_004443"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203690"/>
    <dbReference type="OMA" id="TQPFFSQ"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF63">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="13">
        <location>
            <begin position="84"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN11787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG85615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="528" mass="57291" checksum="AD9A44CCCA870780" modified="2016-07-06" version="1">MQGSAVMGCVQQPVWAKGSLGFAHKGSVGAVGLPHQVRLSGLRSCRCSSFEANVVTGRAASSVSVAAPEIGGDGRGFKDNPLGEVDPEVRSLIDNEKQRQFRSLELIASENFTYRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDELEILCQQRALAAFHLDGEKWGVNVQPLSGSPANFEVYTAILKPHDRLMGLDLPHGGHLSHGFMTPKRRVSGTSVYFESMPYRLNESTGLVDYDMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFECCDIVTTTTHKSLRGPRGGMIFFKKDPVLGIDLESAINNAVFPGLQGGPHNHTIGGLAVCLKYAQTPEFKAYQKQVVSNCRSLAAKLMGQGYELVSDGSDNHLVLVNLRPFNVNGAQVEKILDMASITLNKNSVPGDKSAIVPGGIRIGTPALTTRGFTEKDFMLVADFIHEGVKITQEAKKLAPSPKLQDFLKFITAPDFPLIGQVLDLKRRVEDLATQFPLPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YD11</accession>
    <name>A0A175YD11_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0933462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YD11"/>
    <dbReference type="Gramene" id="KZM81499">
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        <property type="gene ID" value="DCAR_029112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="68" mass="7470" checksum="600C56CDA33E9C58" modified="2016-09-07" version="1">MIRYRGVSDHSDEAKTAAKGAPTTEGQQKKVTLTKKEKAGKDKELLSRGVVLDNNLESIKSQTIELID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDY4</accession>
    <name>A0A175YDY4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0933734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDY4"/>
    <dbReference type="Gramene" id="KZM81683">
        <property type="protein sequence ID" value="KZM81683"/>
        <property type="gene ID" value="DCAR_029296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH14217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="237" mass="25920" checksum="06EA5B195D7708EE" modified="2016-09-07" version="1">MANNMALHWAPPPPGAIKINVHGHTISSPLSNGNSLGIGAIYITSGAEISLLTTSAIPALTPLGNQLWAMYVPLRRAVVQEYDDLRLETDNYEAYMTIKHFHVGVSASIFDIADQVARFLSKLGMDHASKFTPSTCLLGVWRSFWIGTMGLHHPSYMDTFIPSNAPNPIDFNVSLSLTEQVDDLGLGQLDAPRVSRIELEPEELEDLMEGAVEDSFVGSLGPRRSLLAHEPPFPDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGP1</accession>
    <name>A0A175YGP1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor HY5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_030415</name>
        <name evidence="15" type="ORF">DCAR_0935335</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGP1"/>
    <dbReference type="STRING" id="79200.A0A175YGP1"/>
    <dbReference type="Gramene" id="KZM82846">
        <property type="protein sequence ID" value="KZM82846"/>
        <property type="gene ID" value="DCAR_030415"/>
    </dbReference>
    <dbReference type="OMA" id="RKVIMNT"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
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        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF14">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23708" checksum="B420AFA7356280BB" modified="2024-05-29" version="1">MSTSPAPCSRSWSISNDSLRRYVHFATSDCNRVGNENDGWKLLAFETELRYQRRSGSFDTFRSRWLLRSISPQQFITGANAIDAAKQWDADLVEARYIKDLEDNRSIYDKIRGEPKPPLETENSLCGCSSFTPKEIAAGLHPKQNNAIRGLLLQSGWVVEKLENDSCIVTYVAQILQDGCPGFCESLNTKLVMIIENLQKQAQACSVHVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W434</accession>
    <name>A0AAF0W434_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0101897</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W434"/>
    <dbReference type="KEGG" id="dcr:108193961"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF467">
        <property type="entry name" value="CARBOXYLESTERASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="71"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG82729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="317" mass="35481" checksum="49A77FC6CA2598BE" modified="2024-05-29" version="1">MAELDYELPRFIRVYKDGTVERLMGTDTVPAGIDPETGVESKDVLVTPESGVSARLYLPKIRDPAKKLPLVIYFHGGGFYCFTPFSTFYQPFLNSFVAESNIILISIDYRRPPEHPVPVAYDDSWNAIRWVASHCNGTGPEAWLSEYADFGRVHISGDSAGANIAHCMAMRAGSEKVNGLDFDGLILMHPYFWGKDPVGGETDDTEKRAYIEKFWVLACPTSSGADDPWMNPGLDPKLSSLVCKRALVFGTEDDFLKYRAKYYGEVLGKSGWAGKVEVVETEGEAHVFHLYKPKIKKSMELVHKVMSFIYEENANPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W4N9</accession>
    <name>A0AAF0W4N9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0102097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4N9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="23616" checksum="51818847383A72FD" modified="2024-05-29" version="1">MNWCIFLKGTGHPSISSFRYFIMLKLLMSSIGGENLATRLSTSRSGMEKKWSNGVQQEINEKDSRTESKIIEDIVTSFMQKYSEHYPRGQTSQQNCSQVMPLTESYRFDIEEGSVEVTKGNANPMKNSYGDMTFRSWMKDWMIQNPKFFILVVIIVIVILIFTVVTFIQIGIEASTADAVTSAFKDFPKTLNDVEPKNFRTTFPGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5J1</accession>
    <name>A0AAF0W5J1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0101157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5J1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF16">
        <property type="entry name" value="OS02G0565500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="68"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG81998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="29418" checksum="B053AF3AEF69C6FC" modified="2024-05-29" version="1">MRSSPTVRKQKKLNQKTAKRPEKKQRKIRGRGEPRGSGMVRGGGANKVLPIETPALPLDELNKITGNFGRKSLIGEGSYGRVFYANLNNRASAAIKKLHTGSSQETDSDFTAQENANNNKILAYQYATIGSLHDVLHGRKGVQGAEPGPVHSWVERVKIAYGAARGLEFLHEKVQPPIVHRDVRSSNVLLSDDFFVSCRSFPVLLTMLLLRLLQSSRLMLNSNVWPNFTSIFKMVARLPNFQKRGPTLSPLFKRMAAVKETLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6S1</accession>
    <name>A0AAF0W6S1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein BOP1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0101841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6S1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="121"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="380"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG82675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="722" mass="82340" checksum="2D8CD137423B5A59" modified="2024-05-29" version="1">MKAPKSKQESKSKEKEPQQHVVEEEDPVVSDSDDEDWSVEEGSEGSLSDSGLENSDFTDNKEDLTTSDDAVSLDDEDDDDDVGQDSQSESGESHQGVEESDSSEDEAAPRNTIGDVPLKWYEDEEHIGYDISGKKLKKKEKQDKLESFLERADLKNWRKIYDEYNDEEVEITPKEAKIIRRLLKGKAPHAEFDPHAPYVDWFAWDGAKHPMSNAPEPKRRFIESKWEHKKIVKLIRAIRNGYIKFDKPKEEPKVYNLWGDDQTEKAGGLAYIPAPKPQLPGHEESYNPSLEFIPTQEELDAYQLEFEEDRPKFIPKRFLSLRSVPAYEKAVKETFDRCLDLYLCPRARKKRINIDPESLKPKLPSRKDLRPYPSTCYLEYRGHKGPVMSISTDPSGQWIASGSSDGTVRIWEIDTGRCLKIWELDESVNFVAWNPSLELPILAVAVGADVYLLNAGLGREEQHKIEELLRVEKPEAPAPDESSKGAPTVIWTEDEKQKGIRLKHIKTVTSVEWHRKGDYFSSVMPSDILVIESRAISVHQLSKKFTQRIPFKLHGLPVSTAFHPSRSFFFISTKMTVRVYDLLKHELVKKLETGLRNVSSIAIHPGGDNVLVGSREGKLCWFDMDLSSQPYKILRCHKKDITSVAYHRTYPLFASCSDDGTAYVFHGMVYSDLNQNPLIVPLEILRGHHSSNGRGVMDCKFHPRQPWLFTAGADSVIKLFCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9D7</accession>
    <name>A0AAF0W9D7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0103008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9D7"/>
    <dbReference type="KEGG" id="dcr:108205020"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000002">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000040">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
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            <begin position="113"/>
            <end position="156"/>
        </location>
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            <begin position="324"/>
            <end position="367"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG83830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="527" mass="58063" checksum="DE7BBACC12386B03" modified="2024-05-29" version="1">MSLRPNARTEVRRSRYKVAVDAEEGRRRREDNMVEIRKNKREENLLKKRREGLQAQQFTATAAQLAQKLESIPAMVAGVWSDDGNLRLEATTQFRKLLSIERNPPIQEVIQSGVVPRFIDFLGLDEFPQLQFEAAWALTNIASGTSEHTKVVIEHGAIPIFIRLLASPSDDVREQAVWALGNVAGDSPKCRDLVLSQGALIPLLAQFNENAKLSMLRNATWTLSNFCRGKPQPHFEQTKPALPALARLIHSNDEEVLTDACWALSYLSDGTNDKIQAVIDAGVCPRLVELLLHPSASVLIPALRTVGNIVTGDDMQTQVIVQHQALLCLLNLLTNNHKKSIKKEACWTISNITAGNKEQIQAVIESGITAPLIQLLENAEFEIKKEAAWAVSNATSGGTNEQIKFLVNQGCIKPLCDLLVCPDARIITVCLEGLENILKVGEAEKNLGTTGDVNLYAQLIDEAEGLEKIENLQSHDNTEIYEKAVKVLETYWLEEDDEQLPVGDTPQSGLDFAGNAPAVPSGGFSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9P3</accession>
    <name>A0AAF0W9P3_DAUCS</name>
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        <recommendedName>
            <fullName evidence="6">MHD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0105075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="776"/>
        </location>
    </feature>
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            <end position="1040"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1153" mass="131290" checksum="66703C7D3FD1481B" modified="2024-05-29" version="1">METTPSLLQRYRRDRRKLIKFLLSPAAGFVKELRNSSNSISSVDYDTLSADYILHCIQSNGILDISEATRKFTEEAQYPITMDSELGDTYFLVSDPESAGSPPHRAPPPIVVNHSSSNDLHPSDLHDSLIAQKISVPSHESKDKYAANKQKWKPVNRVLSLGLPALHTGLSDDDLRESAYEILLTCMAFYGLEIYIFEDQKKDKSSGFLAGLKNKKDKKHPRSQSQSPERHDEVINTIRVQMQISEAMDACVRQTSMQFASRKACGQLDIPQISLGLLNGIFKSDFLYEKPYMQWKKRQANILEEVFISANHLITDKQVIDTLLAKMNNSEEWDNLMSTSERTEVLAAIRQVSLLLSSVNGHFGIQGETCYWATSYHFNIRLYEKLLFGIFDILDEGNFIEEADEFLKLCKLTWSTLGITQKMHNALYGWVLFQQFVLTEEEMLLDHAIIEVQKLLSAESSRGKDEEYINSLVCYIECDDSKSEMSLVQAILWSITYWCDSKLQDYHLHFSEEHDFFAKVLTLASVLRNKNYGAQDENKLTSDALSGPDIVHVKMYVERSVEAAYRRIEDNMNLGSQMERGNALAVLANDVRVIAEKELNLYSPVLCDWYPESAMVAAKLLHQLYGERLKPFLEQVSSLSDEVKLVLPAVGSLEHGLTLLFSSTCKANESRSFSSLELDRYQVGKFSRPIILEWVIAQHARILEWTGRILDLEDWEPLSYQQKHSSSAVEVFRIIEETVDQFFGLRLPLDITHLQALLSIIFHTLDSYLHKVCSHIVDKHHLYPPVPPLTRYKEAVFPMIKKKVVENVLLEEEINGNLVELTVSKLCIRLNTLQYIQKQMGILEDGIRKSWGSATISDNSRGCEESRETSETVSEYSESVDELFVATFDSLRDTAADAIKRTCEFIGARVVFWDMRDTFLFRLYRGSVGSARFDNILPNFDSVLNNICDYVDDVLRDLVVASICKASLDGFVWVLLDGGPSCAFSDADINMMEEDLNMLKDFFIADGEGLPRSLVENEAQFAHQILNLYSLQTESVIQMLMTASELIPTGVESHKHRHRHLVDAHTLVRVLCHKKDKEASQFLKLHYQLPASSDYEDESPLKEPGLKPPMMADLLRNTSMQWSNNGHDSFKSIKKKFQKVTSEIRHSGSLSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WA64</accession>
    <name>A0AAF0WA64_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">PLAC8 family protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0105012</name>
    </gene>
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            <taxon>Apiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31152:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23270" checksum="D4C3A598FC1FCDDB" modified="2024-05-29" version="1">MASSKAQVEKAQLRQTYRNEWHSDLISTITADDCVMCNEFLECVVYVNVPQCASYMLRKRALYDDMSRYVCCGGYMPCSGRCGESKCPEFCLCTEVFCCFANSVVSTRFLLQDQFNIHTTECDNCIIGFMVCLQQLACICSLIAMVAGSEEISEASQLLNCISDWVYCTVCACMQTQHKIEMDKRDGLLSSQPMAVPPPQQMSRVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAQ5</accession>
    <name>A0AAF0WAQ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ferrochelatase</fullName>
            <ecNumber evidence="9">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0104805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Plastid</location>
            <location evidence="1 9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="9"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAQ5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000006">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF4">
        <property type="entry name" value="FERROCHELATASE-1, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="7 9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG85614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="490" mass="54037" checksum="0BF75AE9A72306B0" modified="2024-05-29" version="1">MDAIASSRILPQLKGSDRSNLSLSNRKFMSSSRSTLVMPTCKQSYEGECTSKVSTQKVSVLSVSGSTEQRNSMLGGLELKGSVSAKPLVGKVFSSAGSCTYPGSSIESHAHVAEEKIGVLLLNLGGPDTLQDVQPFLFNLFADPDIIRLPRLFRFLQRPLAQLISVVRAPKSQEGYAAIGGGSPLRKITDEQANALKMELEAKEVPVNVYVAMRYWHPFTEEAVQQIKRDGISKLVVLPLYPQYSISTTGSSVRVLEQMFREDATLSRMPVSIIQSWYQREGYVKSMADLIGKELKSFSNPEEVMIFFSAHGVPVTYVEDAGDPYRDQMEECIYLIMQELKSRGINNNHKLAYQSRVGPVQWLKPYTDEVLVELGQNGVKSLLAVPVSFVSEHIETLEEIDMEYRELALESGIENWGRVPALGCTSSFITDLADAVIEALPSATAMSAASTSDDSELDPLGYAIKMFFGSILGFFLLLSPKIKSAFRNHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WC38</accession>
    <name>A0AAF0WC38_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0205909</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000157">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="538"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="613"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="680"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="763"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1184"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1214"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1260"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1297"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1325"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1355"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1410"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="170"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="838"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG86691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1438" mass="161894" checksum="5377AE9325152637" modified="2024-05-29" version="1">MAALDGSNEKESLRTGSDVGRSLRSNFGHLSSSFRNIITLSSLDDNGDDQKSQECAEFSKLSSSKCSRSSSFDEDNKNTPDAKMKQGVDVTKLDALERHMLIEKLIKDAKNDNLQLLQKIRKRMDKVGVKLPTVEVKYTNLHVEAECEVVHGKPLPTLWNSLQSMVSDFAKLPRLKPKAAKIDIIDDISGIIKPGRITLLLGPPGCGKTSFLKALSGNLDKSLKMTGNISYNGYNLKDIVPQKTSAYVSQNDLHIPEMTVRETLDFSACCQGTGNRAEIMNEISRKEKEAGVFPDPDIDVFMKAISVEGQKETLQTDYILKILGLDTCADVLVGNAMKRGISGGEKKRLTTGEMIVGPSKASFMDEISNGLDSSTTYQITSCLQQLAHITDATILVSLLQPAPETFDIFDDIILMAEGKIVYHGPCSRILEFFEGCGFRCPERKGVADFLQEVISRKDQAQYWHQSAHSHSYVSVRTFSEKFKQSPFGKKLDKDILEKNVMSENHDNSISFRLFSLSKWTLFKACMSREFLLMRRNSFVYIFKSAQLIIIASVTMTVFIRSRIKINALDADKYLGALFYSLVIILVDGFPEVSMTVARLAVFYKQRELCFYPAWGYAISAAILKVPLSLLEAFIWVCLTYYVIGYSPEPERFFRHLILLFAVHWTSISMFRFIASLLRTVVASTTTASLSILVVLLFGGFIIPKSSMPFWLEWAFWLSPLSYGEIGLAINEFHAPRWQKLGSTNTTIGITTLENRSLNFDGHFFWISISVLFGFILFFNVGFLLALSFLNPPGSRAIVSSVKLSQIQRSEESTNTDYHDEEKCSNRGKAVLPFEPTSLVFQDVQYYVDAPTEMRERGFSQKKLQILHDITGALRPGVLTALMGVTGAGKTTLLDVLAGRKTCGTIKGEIKVGGYPKIQETFARISGYCEQTDIHSPQVTVEESIIFSAWMRLHPQINAQTKYNFVKDVLQTVELDGLKDALVGIPSVSGLSTEQRKRLTIAVELVANPSILFMDEPTTGLDARAAAIVMRAVKNVADTGRTIVCTIHQPSIDIFEAFDELILLKSGGRVIYSGSLGQNSSNVIEYFERIPGVASINKNYNPATWMLEVTSKSAEAELGLDFAQVYQSSALHERNKEMVKSLSIPPSGSVDLLFPTRFSQNSWGQFKSCLWKQHLSYWRSPSYNLMRLMFMIFSSFLFGLVFWDQGKKINNQQSLFNMLGLIFSATIFCGINNSSSVIPYITTERNVLYRERFAGMYAPWAYGLAQVTIEVPYIFVLSFVYVIITYPMIGYYWSAYKVLWYLYSIFCTLMYFTYMGMMLVSLTPSYPLAAIISSSSYTMLNLFSGFIIPQPQIPKWWLWLYYLMPTSWTLNGMLSSQYGDLEDEIIVFGETKTVAAFLEDYFRFHRHRLPLVGAMLILYPIVFASIFAYAIGRFNFQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDA6</accession>
    <name>A0AAF0WDA6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206229</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDA6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF37">
        <property type="entry name" value="INACTIVE PROTEIN KINASE SELMODRAFT_444075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="240"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG87009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="626" mass="70382" checksum="C5E67C04C23196D6" modified="2024-05-29" version="1">MPSEVILVAVDASKEVTDYTLEWAVQNVIKPKDSLILLAILPSTSSPHDLVFFKTIGVLKKKCNRRENTRNLKEKIAEENCRDQLVQDTHDSCTQIIRQLLKLHSIVQVRTEVRVVADQAAEGSITKLALEIGATWVILDRISIHTHPPQVAPRISIDTKLSRPKQPMSGEQNLRTETNQTTEVAKIDRLSSIRKAMSVSVKQPPIPPPLCSVCKNNAPIFGRAPRKFTYMEIQEATNGFSKNNFLAKGGFGDVYKGVLDDGQVVAVKQHKVLSAQGASEFCSEVEVLSCAQHKNLVILVGYCTEMEWLLVYEFVCHGSLDKHLYGRGEPMAWQNRMKVAVGSAGGLRYLHEDCRVGCIVHRDFRPNNILLTHDFESMVGDFGLARWQADGQSAEETRIVGAFGYLAPEYTQTGLITEKADVYAFGVVLLELLTGIKATEFARNAKQPYMPDWSRQFLESKVPSEIVDPRLDHDFVEKEVECMIQAASLCISPDPDQRPRMSEVLKILEGYMPRVVPGPHSQPASTFPGRNLIEDHIDNKSEILKLQRNRLVYAGISDDKCNPLHQVLKDTQLQSMYKQVSFSDENKIAGTANKMDKNKSKLPRNEDYQEYLQGSLSRYIQNMKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WDV7</accession>
    <name>A0AAF0WDV7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0206200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM135 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WDV7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000102">
        <property type="entry name" value="Putative serine esterase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="475"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG86981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="755" mass="85865" checksum="E5C2C686CD320588" modified="2024-05-29" version="1">MFEAVHEIAIYIHRFHNLDLFQQGWYQLKITMRWEDGNSSSVGFPSRVVQYEAPDLGSDDVYGVWKIDDTDHSFSSQPFRIRYARQDVLLSLMVSFNLPHDISKGVSTTAVILKFELMYAAVLGNRSTLQASLDVPAAVHEFRIPPKALLGLHSYCPVHFDSFHSVLVDISLHITLLKGGVHNSSTKPPSVFWADNGGAGGNYDRLKQVVLVKALFGARDLFLEDLHKLSKGISKTINFTNLTSKFNEKNLPPKVGLGTSDAEVRKLASSISLNLVEKDNGNIDFRSDFIQSLSRDELFAAFHSLGDQLLYLWNVFLQFHRANKIKILEILRDTWASDRKAEWSIWMVYSKVEMPNQYISSDIDGASQQALRRKALALRKLNEDPAQAAATRAELHRRSIAQMRINNRSIQDMHIFGDPSRVPIIIVERVANAPRRTASGDSYFSYLDQKHTFGMQSETGSKSVKKLSGTAHKSSRNLKIAIFVHGFQGHHLDLRLVRNQWLLMDPKIEFLMSEANEEKTSGDFREMGRRLAQEVVSFVKKKMDKASRSGVLNSIKLSFVGHSIGNVILRTALAEDIMEPYLRYLYTYVSISGPHLGYLYSSNSLFNSGLWLLKKLKGTQVIHQLTFSDDVDLRNTFFYKLSKQKTLDNFKNIILMSSPQDGYVPYHSARMEMCQASSGDYSKKGKVFLEMLNNCLDQIRAPSSEQRVFMRCDVNFDISLQGRNLNTFIGRAAHIEFLETDSFARFIMWSFPELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WER0</accession>
    <name>A0AAF0WER0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Peroxidase</fullName>
            <ecNumber evidence="4 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0207416</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
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    </reference>
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        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0349">Heme</keyword>
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    <keyword evidence="11 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041771033" description="Peroxidase" evidence="19">
        <location>
            <begin position="24"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
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            <begin position="41"/>
            <end position="346"/>
        </location>
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        </location>
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    <feature type="binding site" evidence="16">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="86"/>
        </location>
        <ligand>
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            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="88"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <location>
            <position position="106"/>
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        <ligand>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
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            <dbReference type="ChEBI" id="CHEBI:18248"/>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        <location>
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        <ligand>
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        <ligand>
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        </ligand>
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        </location>
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        </location>
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    <feature type="disulfide bond" evidence="18">
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    </feature>
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        </location>
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    <feature type="disulfide bond" evidence="18">
        <location>
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            <end position="251"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apioideae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971">
        <property type="entry name" value="TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="6">
        <location>
            <begin position="31"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG87880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="212" mass="23538" checksum="4E778986887A6DDE" modified="2024-05-29" version="1">MEASVAETGRDNCLPTGVGEAVKQVSAEKILQVVLVSPQIPGNTGCIARTCAASAVGLHLVEPLGFQVDDTKLKRAGLDYWPYVVVKVHSSWEEFREYFRQQKGEKRLLAFTKRGTNIHSDFSYRRGDWLVFGAETFGLPAEALLDCKNEPLGGGTIRIPMVETYVRCLNLSVSVGISLYEACRQLDYEQLQNPTEISNDKQKSFVSEDVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHA3</accession>
    <name>A0AAF0WHA3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHA3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF14">
        <property type="entry name" value="PROTEIN PMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="7">
        <location>
            <begin position="61"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="39106" checksum="A255D7BCC25259B8" modified="2024-05-29" version="1">MIQANQTSCALFIGTWVYDDTYPVYQSSACSIIDPEFNCQMFGRPDTDYLKYRWKPVNCELPRFDGEEFLIKMRGKNIMFVGDSLGRNQWESLICMISASVPRSPTQFIRGDPFSSFKFIEYGVAVQFYRAPYLVDIDLVQGKRILKLEDMSKNGGAWKNADVLSFNTGHWWTHKGSLQGWDYMEAEGQLYQDMERLVALERGLRTWAKWVDANVDKSKTRVFFQSISPTHYNPSEWSAGATSVAKSCYGEKTPMSGTAYPGAYPGQMKVLDAVIRNMSNPAYILDITMLSAMRKDAHPSIYSGDLSPEQRANPDHSADCSHWCLPGLPDTWNQLFYTALFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WI65</accession>
    <name>A0AAF0WI65_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208023</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WI65"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="119"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="414"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG88788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="588" mass="64868" checksum="E67C808A53A83F17" modified="2024-05-29" version="1">MLYSTSSAPILMKSSISLLNLVKLTSFSSSAPKFILPIKQISTISSFSTSSSSSLVLTLSGSTRRRVRVFSTATAAAEDGTFFAEKNVSWVSLGISDRLSQALCSIGLDRPSLVQAACIPFIKSKNDVVVAAETGSGKTHGYLVPLIDKLCTALENSNEDTPVEGQLTQLHSHSLVLCPNVMLCEQVVRMANCICKDSGEPLLRAAAVCGRQGWPAEEPDIVVSTPAALLNYLYAIDPDRRRRSNFMRGVKYVVFDEADMLLCGSFQNQVIRLINMFRFDEKLLSREKSSEAERLLDSNSDFITQFEEEESENLNPDSILKEEEAEIVQVVQNKGEENEVGVVKRRDWRRVRKTYERSKQYIFVAATLPENGKRTAGGVLKKMFPDASWVSGSYLHCHSPRLVQNWVEVTVDTQVDILIEAVNQGSNTGNEVSRTMVFANTVEAVEAVANILSGAGIHCFRYHRDGSLEERAQNLVDFQQKGGVFVCTDAAARGLDVQNVSHVIQAEFASSAVDFIHRVGRTARAGQPGLVTSLYTESNKDLVAAVRQAGKLGQPVEKAFSRKRSFRNKIKKRVKSSTWSLHGCHLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WID0</accession>
    <name>A0AAF0WID0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0209472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WID0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415:SF52">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG90229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26576" checksum="4DA79EB70BE63FF5" modified="2024-05-29" version="1">MLDPRIQYARCLYWGIVFLFCTPPWIWITLSFADQSSIKFSIQEFHIAGVESSDGSNLNQSIIYFKLNLENDYEELGIYYDNLNLTFSYTTQGDIVPLANYTIPGFRQGRDAETDREGYVVTTRGITLQEISNVSVSSNVDFRVDLATAVRFRHYIAGIKSKRLQTMAWCNVEVDRITGKKKNNNAIELQHVVIRHPSAWISFAYIIANLSTLVLVCCLVFICFGVCDRLLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJZ7</accession>
    <name>A0AAF0WJZ7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0209002</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJZ7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23791">
        <property type="entry name" value="UBCc_UBE2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000047">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="127" mass="14223" checksum="B3642003C09A84E6" modified="2024-05-29" version="1">MGGDPGISAFPEEDNIFCWKGTITGSKDTVFEGIEYKLSLSFPADYPFKPPKLKFETGCFHPNVDIRGNICLGILQDKWSSAYDVRIIQSLLGEPNTSSPLNTQAAALWENQEEYRKMVEKLYKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKD1</accession>
    <name>A0AAF0WKD1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0209082</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKD1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000316">
        <property type="entry name" value="serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF73">
        <property type="entry name" value="OS01G0201200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="42"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG89843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="348" mass="39391" checksum="742600B5FB058E68" modified="2024-05-29" version="1">MKIYDKLKSKKESAWTKFFDHGGGKVTSVESADEWMVDLSKLFIGLRFAHGAHSQLYHGCYKDESVAVKMIRVPDDIDDGSLGARLEKQFTREVTLLSRLHHQNVIKFKGACRKPPVFCIITEYLSEGSLRAYLHKLEEILPLRKVVSMALDIARGMAFIHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDPLADDSGTYRWMAPEMIKRKSYSRKVDVYSFGLILWEMVAGNIPYEDMTPIQAAFAVVNKNFRPAIPGDCPAAMKALIEQCWSLQPDRRPDFCQVVKVLEQFEASLASDGNLNKLQNLPCQDHKKGILHLIQKIGPVHHNPSPKPKPKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKT3</accession>
    <name>A0AAF0WKT3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310982</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKT3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041921410" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG91732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="41834" checksum="DF5877C57ABC0D00" modified="2024-05-29" version="1">MDSNKPFSKVSVILFLVIFCDKVCSYDTSRVKKHGDNAHEKFYDINSLLDSWQEACQSESGGIVSVPDGTFQLDPIEFEGPCKNQVTFQLDGTLQAPTGIINGDDWIKFHNIDGLIIQGSGTLDGQGASAWQDKCPSCPPLTTSLTLSSVTNAQVTGITSLNSKGYHIKVNKGGGTTIEHVTITAPEDSPNTDGIHTSEANNINILNSDIGTGDDCISIGEGTQNINITGINCGPGHGISIGSIGKNADDGSVSGVHVMSCTMTSTENGVRIKTWTSDCSATVSDVTFHDITIDQASNPIIIDQQYCGGSHEYPSHVQVSDVKFINVHGISKSEVAINLQCSSTMPCQGIELDTIDLSLDGGEQTTASCSNVNATYDGSQNPPPCSNSLYHRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WN83</accession>
    <name>A0AAF0WN83_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0311720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WN83"/>
    <dbReference type="KEGG" id="dcr:108212382"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF86">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG92451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="157" mass="18033" checksum="51174914344085B3" modified="2024-05-29" version="1">MVILGKFELASGQKVNRSKSSIFFSTNMNLDKKQQVCDIVQMMEANDNCTYLGLPNMMQHSKTTTLKFLKDQVIRKALSWDVKIIFKGKKEVLVKSVIQSFPTYAMSVSLLPLEITKDLETSISWFWWISKKNDSGSIHWMSYERLSRHNSCLGDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPR9</accession>
    <name>A0AAF0WPR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0311740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPR9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710">
        <property type="entry name" value="BNAC02G09200D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33710:SF64">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG92471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="303" mass="34712" checksum="B0A8F1EC312CB389" modified="2024-05-29" version="1">MPYPFGWIESPSTGLSGGLLTLWNKSIFSISFSKVATNWLLIRGSCLADGRYILIINFYAPQPLASKKHIWAELLHELGEATETFIIMMGDFNAVHKVEERVNSVFCVHESSAFNDFIEDTGLIDVHLVNSMFTWFGPANKCSRLDRILVSHDWLSLGNWSLTAQDRVSSDHRPIVLTINQLDWGPKPFKLFNCWLEDPHFVETLRNSWLNCRLNGFTQKFRRLREVAKKWGNQEFGSIDEKIKALLTKQDKADAGLEHSLDGKQIKVELDRLIQVKVGVLCQKSRLNWELKGKKTQNFFTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WR93</accession>
    <name>A0AAF0WR93_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caffeoyl-CoA O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0313580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WR93"/>
    <dbReference type="KEGG" id="dcr:108213052"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF82">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG94287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26569" checksum="A0C8B8C83D32473B" modified="2024-05-29" version="1">MENEKKELTVPNKGLLQTPELYQYILESSVYPNEPEPLKELRVVTASHPRALMGTAPDAGQMMSMLLQLLNAKKTIEIGVFTGYSLLLTALTIPEDGEIIAVDLNLDTYEIGLPIIRKAGVEHKINFIQSEALPVLDKLLETSGNEGSFDFAYVDADKINYRNYHERLLKLLKVGGVVVYDNTLWGGSVAMPEEHVAQEWKAGRHWTIELNKFLASDSRVQICLAPLGDGMTICKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WRD9</accession>
    <name>A0AAF0WRD9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="8 9">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0313967</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="8 9"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WRD9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010025">
        <property type="term" value="P:wax biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF6">
        <property type="entry name" value="LONG CHAIN ACYL-COA SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0276">Fatty acid metabolism</keyword>
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        <location>
            <begin position="51"/>
            <end position="480"/>
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            <dbReference type="ARBA" id="ARBA00004930"/>
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            <dbReference type="ARBA" id="ARBA00006432"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG94670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="660" mass="74448" checksum="64B2A7F38EE43DB5" modified="2024-05-29" version="1">MSLFAVKTEDGRKGQDGQPSVGPVYRNPLAKDGFPPLDPELSTTWDVFSASVKKYPGNRMLGWREFINGKWGPYVWKTYEDVYNEVLHVGAALRASGIEPGSRVGIYGMNCPQWIVAMEACSAHSLICVPLYDTLGSSAVDYILDHAEIDIVFVQDKKLKELLKPECTHARRLKSIISFSSLMESEKDGALAIGIKPYTWKDFLLMGRESPAAIVPPKPFNICTIMYTSGTSGEPKGVILTHENISTYIRGIDIFLEQFEDKMTVDDVYISFLPLAHILDRMIEEFFFYRGASVGFYHGEIDALKDDLLELKPTFLAAVPRVLERMHEGILKVVEDLNPRRRKIFSILYKYKLKWMKLGYKHRDASPVADLLAFRKVSAGLGGRLRLIVSGSAPLSNEIEEFMRVTSRAFVVQGYGLTETCGLASIGYPDEMCMIGTVGSAFVYSELRLEEVPDMGYDPLADPPRGEVCVRARSKFAGYYKNQELTNESMKDGWFHTGDIGEMLPNGVIKIIDRKKNLIKLSQGEYVAIEHLEKVYCITPIVEDIWVYGDSFKSAVVAVVVPHKGNTERWAQQNGHKGSFSQLCSLKDLTESVLQELKSTAERNKLRGFEYIKGVHLVPEPFEAEHDLVTPTLKKKRDKMLICYKVQIDELYQKLQKGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WSM0</accession>
    <name>A0AAF0WSM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0312623</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OMA" id="ACKFERD"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
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        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
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            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG93340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="152" mass="17723" checksum="80E5FFA32B73C017" modified="2024-05-29" version="1">MKSESPNIKSCGSCSSDSVKNWEGRLCFCGKQARVYTSWTLKNPGRRFYTCSTPKEIQGCHFFEWIDEGFSGRAFDVITHLNHRRIFLEEKLKHVEEDLEENIEKRRTLKDEKLQLIDGNKAFEADLKLIRRQLKLCVLVCVLLFAVVLMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WSN4</accession>
    <name>A0AAF0WSN4_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0313120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108214744"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000131">
        <property type="entry name" value="la-related protein 1B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF132">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="335"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="502" mass="53783" checksum="A10E6BFCF293A0AD" modified="2024-05-29" version="1">MAAADTSLSSSSSSSVSMTSPSWTQQEEAPPVISVVIGGESGNQDNSNAGGLKQVWGKPVNVNGSSGGAAVLSAAVATVDVSSRASEAPAAGVVMDTHSWPALSKAPPKSASSDSVPTSHSGSGMGSISSSKQVIGNTSSPNSNVNHVTNTRQRSIKRGTNNGGFIQPSSPQPQAPAVEVLPGKPGITTGESSSHKEGGPKGGFGNDHQPQRSNSFNRRGGGGQHPRGDASYHHSSGGRRDQDRGNQDWNHTQQSYGSRDNYMQQQRGAHRGYPRGPHASPFIAPQPLPVRPYPNHMVFPTDVPQPVFYVQSPESLRGVVPIMPQVAAHPMYYPPIPDPQLHVKIMNQIEYYFSNENLVKDTFLRRNMDEQGWVPIKLIAGFKKVMNLTDNILLILNAVQASTVVEVQNDRIRKRNEWMKWIMPPGPFSPVSSPQARAKSGHDMLTGQFQGVTLDERSVHGHTDMFKKSLSGEFSSQLQHSGGEGDVHSGFEHKSSAGSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X0D2</accession>
    <name>A0AAF0X0D2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0418094</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
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        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
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        <location>
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        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG98749.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
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            <name>heme</name>
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    <comment type="similarity">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <location>
            <position position="449"/>
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        </source>
    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1V6</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cux N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <name evidence="5" type="ORF">DCAR_0418339</name>
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    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG98993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108215670"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF13">
        <property type="entry name" value="REMORIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </feature>
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            <end position="66"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="118" mass="13988" checksum="D465255ABD30FA97" modified="2024-05-29" version="1">MPPRRKNVRDIELEELRRQVEQLQEQLAQREGRRHNADNHNSENGSSTPDDEDENPFHEESDDHSSHASAPRRRHEFIDWINKVERVFEYNDVHDQQKVKLVAVKLKKYDGLFMVGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="3 4">
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    <dbReference type="InterPro" id="IPR005162">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33223:SF10">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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        <name evidence="2" type="ORF">DCAR_0520027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Scandiceae</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <tissue evidence="2">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X5F9"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="113"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="162" mass="18782" checksum="322C879633B7C4F4" modified="2024-05-29" version="1">MNFQAETNGKDPTPADVYLLTHTKNRDKKTFVTKKAEAVYVIEIREERSKPVEGSEEPQIIDEDEIFLQAVGGLDKRNRIYGMGSLQSVIYGPESRSDTSTSRYSGSNFNREYELMQVELQEMKDQVKELQEMRNTELEDMRKQMEEMKSQLALVFRNQNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0X5P5_DAUCS</name>
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            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
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    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
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        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH01903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108220225"/>
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    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH01903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="267" mass="30356" checksum="4D2B642714ADABA5" modified="2024-05-29" version="1">MGYAQLVIGPAGSGKSTYCASLHQHCETIGRAVHIVNLDPAAENFDYPVAMDIKELISLDDVMEELGLGPNGALIYCMEHLEESLDDWLEEELENFAEDDYIVFDCPGQIELYSHVPVFKNFVEHLKRKNINVCVVYLLDSQFMTDVAKFISGCMASLSAMIQLELPHVNILSKMDLVRNKKDIEDYLNPESQFLLSELNQRMAPRFGKLNKALIELVDQYNMVSFVPLDLRKKGSIQYILSQIDMCIQYGEDADVKIRDFDPEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X6Q2</accession>
    <name>A0AAF0X6Q2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520838</name>
    </gene>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="45508" checksum="D56F00981F543638" modified="2024-05-29" version="1">MFYSIIISYPKVTKYIFYFSILPSIILRSSNIHNHSKLGKILEIIAGNIDLFLLLNISMEANSNTTRSRREKNLTSKEKQVIYEFLLKKSEGLIRPHTNAVKPTLTEDNKRTRLEFCLSQIDPDTLLFSDMFNVVHIDEKWFYMCKTTTKYYLVHDEPEPTRRVKSKRFITKAMFLAAVARPRNGFSGKIGIFPFTFIEPAKRNSKNRVAGTMVTKVDESITKEKVKSCLIEKVIPAIHAKWPQGMRTIIIQQDNAKPHIDCNNAEFMQAASKDGFDIRLICQPPNSPDLNVLDLGFFRSIQDLQHEKAPTTIEELVAAVKQSFDEYPVEKLNRVFLTQQTCMKEIMKFKGDNDYKIPHMGKDRLEREGTLPLQIRCESDLVNDVVAYLEEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X7R2</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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</entry>
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                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
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            <location evidence="1 9">Nucleus</location>
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        <text evidence="2">Belongs to the CONSTANS family.</text>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEL4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF163">
        <property type="entry name" value="VESICLE-ASSOCIATED PROTEIN 1-2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="5">
        <location>
            <begin position="17"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH06821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="223" mass="24660" checksum="2DA79409B7DE63D9" modified="2024-05-29" version="1">MDSTTSTLFQSAHPEGLISIDPLELKFPLELKKQICCSFQLSNKSDKHVAFKVKTTNPKKYSVRPNNGIVLPRSQSNVTVTMQAQKEVPSDMQCNDKFLVQSVAAPVGATLKDITPEMFNKEAGNHVEECKLKVAYVSPPRPPSPVREESEEETSENTTAANELISALTKEKQSIIQENNRLQQELKLLKRGPRRNNGSGGLSIYYVVLIALLGILLGCYISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XK14</accession>
    <name>A0AAF0XK14_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0728890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XK14"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="176"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="423"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="11">
        <location>
            <begin position="673"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="918"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH09433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130761" checksum="CA80FF1C37C5A609" modified="2024-05-29" version="1">MADAIVGDLAAGLIRKLVSLATNQVIQVWKLHEDLETLRQRYELIGALLHDAQTKNIIMSSAKIWFNKLEDVAQVAEAFMDELEYEVTRRKVENRHKVRDFFVPSKNTLLYRSKVAHKIKNINASFDKICKWATDIGLKPVEHLRSTVQHREIRYTQPFEDESLIVGRDDDISFLVNLLCNPNDEGLQVKAILGMGGQGKTTLARMVYNRDAVIKMFPKRMWVTVSDDFDFMMILNQMVVSLTSSPSVLDNAEGLIKNLQEKLKGEKFLLVLDDVWNEKPEEWDKLRNSLLGVGGARGSKIFVTTRKQEVIDAMRCSDPYLIKKLTEEDSWELFKQRAFLNGGVLETEAFVALGKTMVERCGGLPLAIKTLGSLLYSKKTEDEWLLIKNSEIWKSKGVLSSLRLSYDNLPYSSLKRCFAYCSIMPKDHDIYKDELIQIWMALGFLPGDSTVLMEDIGNEYFEILLGNSLFQDVEKEKDQYGYITKCKMHDLVHDLALEVSSNYSTTVNQSHDVNKGSKAAYVRLEGFKDVKPSMFKLRFDTVQALYAEATIFNCVLPKLKYLRVLVLNSFCNEVPGLIGNLKCLKHLDISRTAYSNTSYKLPNHVTRLYNLQTLRISLAHELPENICQLVNLRHLVIGAYTKTRYMFVGIERLTSLQTLPHFVVKKNQNCLVGQLGMLKNLRGSLKLYGLNEVENIEDARKAKLCEKSNVRHLLLKWRNNEDEREEGEYNDEAVLEGLEPHLNLKVLEIEDFMGKKFASWISKMTNMVRIAVTDCSRCEGFPQLGHLPKLREICIKKMENVKVIGNDLCVGLSPAQKAVTSVMYPSVTTLTLRRLPKLEEWVEDVLSKGGKDQIVFPKLEELEISDCPKLRKILNSCFPSLKDLSITNSERILESMYMHVSSLTTLKLQNIRDGGGDSSSSSSSSSSSSSSSCSNLESILKVLLKNNSLSLTSLDLVDCKGLQHLTLGVSLKILSVYDCPNLVSINLVEGSAGLRSLYLSGLPSSLLDGISTQIQSSTLRELSLGPFSDEFPWPFSSSLHSFPNLVWLSLRGTEMVKSIALFEQLQLSTFPALTELFICDFGGMKDLVLSIAKLPSLADLSIQNCKDLERVSLFDESHCLRFLRISECPILKERYRKESGPEWFKIQHIPIIRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XL26</accession>
    <name>A0AAF0XL26_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">DCAR_0729499</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
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            <tissue evidence="20">Leaf</tissue>
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        </reaction>
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</entry>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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                <person name="Sanseverino W."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Putative 2-hydroxyacid dehydrogenase"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF179">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATED"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0521">NADP</keyword>
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            <end position="324"/>
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    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
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            <end position="293"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36384" checksum="C77F127756C3543B" modified="2024-05-29" version="1">MASSPDKISPELPVVLILKPPEVFKVHEELFCQRFHFLRAWESTLPTDQFLTTYAQSIQALFCNSFTPIPAHVIRLLPSLRLIVTSSVGVNHIDLHECRRQGIKVANIAKVFTDDVADIAVGLLIDVLRKVSAGDQYVRSGSRIWSDFPLGYKLGGKRVGIVGLGSIGLEVAKRLEAFGCIVSYNSRMKKPSVPFLHYANVCELALNSDVLIICCSLTEQTYHMINREVLSALGKHGFIVNIARGSIVDEKELVRCLVQGEIGGAGLDVFADEPNVPDDLFELENVVLSPHRGVFTDETMWECYELVSGNLEAFFSNKPLLTMVHNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">DOG1 domain-containing protein</fullName>
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    </gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="49"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH09909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="269" mass="30651" checksum="088F6C51EBC34331" modified="2024-05-29" version="1">MERAFSQHITAHSLKLEQEEVVEVQEKEEQEGDDVAHLVGCISLHKKSEDLSIRKYRSWFQKQKSIAAGREKQMKASMDLDDFIEEQMKHFHAQYSRGVVPTHLEDLSKLLMPKSTPPNELAALSWLGDWRPSSFLDLLRSLSHSLPSLSKSKTTGLVLSKLTHDVRIEEAVIDDKLCLSPFTREISSMMTFPTFSLPYPVVNPLLSSPKITIGPVLITTSVSRDFSPVMCLEQPLSRTHTTLLFLHVPCIHKWIKLSLVPRRSWVQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNW4</accession>
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        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0729966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108196410"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF22">
        <property type="entry name" value="F25A4.25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="329"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH10497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="542" mass="60110" checksum="DCFF25531111EA0C" modified="2024-05-29" version="1">MIPDKWTATVASIWIQCSSGSSYAFGIYSPLLKSSQLYDQSTLDTVSVFKDIGANVGVISGLLYAAVSSRRRGPWLVHLVGAVLCFVGYFFIWLAVTGILDRPPVPVMCVFMFLASQAQTFFNTANVVVAVQNFPEYGGTIVGIMKGFLGLSGAIIIQVYYSLLENNPSSFLLMLALLPSLLPISLMFFVRTYETNDGHDNKHLNHLSSIALILAAYILMLIILENTFTFQPWARIFMFLLLLLLLFSPLKIAINAQREETCRISPTSASRTRLLHNTESIDPKKNYAVGDPIENRDLQLYARQENDGLENHTRQIGEEMNLLQALGTMDFWLLFVAMICGMGSGVATINNISQIGESLNYTSVERSTLVSLWSIWNFLGRFGAGYVSDVYLHKRGWPRPIFMILTLATMASGHLVIASGFPGNLYLGSVIVGVCYGSQWSLMPTITSEIFGVLHLGTIFNTIAVASPIGSYIMSVRVAGYIYDKEASGHRTCYGTHCFMLSYCIFAAVSLLGSLVALILFFRTRRFYTMVLLNRIQHSSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XNW8</accession>
    <name>A0AAF0XNW8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0730001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XNW8"/>
    <dbReference type="KEGG" id="dcr:108193714"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010029">
        <property type="term" value="P:regulation of seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000485">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000442">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF116">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="277"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="421"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="546"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH10532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="686" mass="76438" checksum="FC471F3843EAE7AF" modified="2024-05-29" version="1">MINNFDQNDRRILTFPAIHPCENIYPGTLLESLVTLSRKISNYQTRFFATQRKNSKQAIRQVGILLMFLEELQESRIVLPGSINLCFSELHLTFQRILFLLDDITREGARIWVLMKAHFVASQFLVLVRSVATALDVLEMDSVRVSSEIRELVELVAIQARRVRLELDFEDEEVMKKVIVIINQFENRFEPDQGMIKGVLDYLGIRRWDECHKEVKFLEELSGLMCLDGNEREMPLISSLAGLMRYCRGVIFTNSGFGEGGQVDGRGNVEVEVVSFLNLEDFRCPISLELMTDPVTVSTGQTYDRASIQKWFKSGNLICPKTGERLETTELVANSSLRKIIQQFCADHGVSLAKTRKKNSDISRTIVPGSPAAAEAIRFLSGFLSSRLSYGTDEQKMKAAHEVRLLGKSSIFNRYCLIEAGTVPPLLTLLASSDSPTQENAISAILKLSKHSKGKKQIIENEGLNLIVNVLKKGLKLEARQTAAATIFYLSSVDKYRKMLGETTEAIPALVELIKEGTNCGKKNAVVAIFGLLLYPRNHHRVLAAGTVQVLTNLLSTSDKKDVITDSLAILAKLSESFEGSSMIVQASALSIILRIFKNLASRSGKEYCASILLSLCHNCGADVISVLAKDPGLMTALYSLLSDVDGTAHSNKKARMIINIVHRFHETSTSQLINHQVQEQSIHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPN9</accession>
    <name>A0AAF0XPN9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase At5g45910-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0831404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPN9"/>
    <dbReference type="KEGG" id="dcr:108197799"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF659">
        <property type="entry name" value="GDSL LIPASE_ACYLHYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042175513" description="GDSL esterase/lipase At5g45910-like" evidence="5">
        <location>
            <begin position="21"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH11908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39593" checksum="3DF2B97CB8D2F329" modified="2024-05-29" version="1">MKQFYFLCMLCIASFGAVISTKKYDSIFSFGDSLADTGNFIRTGALAFPVIADLPYGETFFRRATGRCSNGRLIVDFIAEAYGLPYLPPYLAIEEGLTHKNGVNFAVAGATALDAKFYADKKLGHVLWTNNSLSVQLGWFKKLKPTICHTKQECDNYFKKSLFLVGEIGGNDYNYPAFGGGSPEELKALVPLVLEKIISTAGMLIAEGAVELLVPGNLPIGCSSVYLTIFQTPDKSAYDQYGCLKAHNSFAQYHNNQLKLALGRLRKKYPHTKIMYADYYNAAKQYVHTPRHHGFTNGVLVACCGGGGPFNFNNTARCGHTGSEACTNPSTYANWDGIHLTEAAYGYIAKGLINGPFTIPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRV7</accession>
    <name>A0AAF0XRV7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF868 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0832344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH12835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33672" checksum="CDBC5A82442DB3A5" modified="2024-05-29" version="1">MRDFPSCFGENGVQVADNSYSSVSFSKAPQNMVTCIYQCRLLGKSCLIILCWSKNLMGYCLGVEIDDLGGHCVCRVDVKPSLFYKRKGSKCLQVNSAKIDIYWDLSSAKYGAGSQPVEGFYVGVVCKGEMVLLVGDMVKEAIKKANAVPSLFGSTFVSKREHIFGKRVYKSRVQFSDNGQIHHLVIECETTGISDPRLVVRLDSKMVMQVKHLRWTFRGNQTILVDGLPVEVFWDVYNWLFGTSLGSAIFMFQTCSRTEKLWASQTLDPSEPPWPCLQSNRESKSSDPGFALTLYAWKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XSC4_DAUCS</name>
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    </protein>
    <gene>
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; starch biosynthesis.</text>
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    <dbReference type="EMBL" id="CP093350">
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    <dbReference type="GO" id="GO:0009011">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Starch_synth_cat_dom"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="144"/>
            <end position="320"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH12254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="565" mass="64329" checksum="450798ACE49BF278" modified="2024-05-29" version="1">MELSLQVQRPVWCRNVYRDAPHFKLNPFPGFSRTLTQVMLLLSLRKSVACCQRFPYHSQPFPFKVPLDAYMMDFVFSEREDGGVFDNKLGMDYHIPVFGGIAKEPPMHIVHIAVEMAPIAKVKFVSLMNMVLTYLLIFLFRTFGDVATSLSRAVQNLNHTVDIIFPKYDCLNLKQCKLRSAVEEFHFHKSYFWGGTEIKVWFGKVEGLSVYFLEPQNGLFWTGCIYGCKNDGDRFGFFCHAALEYLLQSGSNPDIIHCHDWSSAPVAWLFKDHYMHYGVSKARVVFTIHNLEFGAHLIGKAMAYVDKATTVSPTYSQEVSGNPAISPHLNKFHDILNGIDPDIWDPYNDKVLPVCHTSENVFEGKRAAKEALQQKLGLKRVDLPLVGIITRLTHQKGIHLIKHAICRTLDRGRQVVLLLGSAPDPRIQNDFVNMASHLHSSHNDRARLYFILVPSIFEPCGLTQLTAMRYGSIPIVHKTGGLYDTVFDVDHDKERAQARGLEPNGFNFEGADAAGVDYAVNRAISAWYDGRDWFNSLCKLVMEQDWSWNRPGLDYLELYNAARKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTV8</accession>
    <name>A0AAF0XTV8_DAUCS</name>
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            <fullName evidence="3">Dipeptidylpeptidase IV N-terminal domain-containing protein</fullName>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OS03G0314500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH14261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="715" mass="80296" checksum="22B4FDAB79F7C6C8" modified="2024-05-29" version="1">MAEKRGSIAFFTTYRPPVPLEIFSCPYPPTSNKDELPMTDGVSYNFNGHSIPPAALKTILRRPKLVPEGIKDTDVDSGRVSGLVFVSERDNLETIQFALRFHDQKPIKTKVFSFADVYSTSDKARMEDSPCIAGNYLVYVSTKEAADKPRQPWTAVYKTNLSDGKTERLTPSFVADLSPSVSPSGKMIAMASFQNRSGWDGEIQDLKTDIYVMFVDKPSNRRVIRRTMILKNGGWPTWGSEDVIFFHRKVGDYWAVFRFDMNTKEQTRVTPDKCNAMTPVAIDANTVAVATIFDIAKFGVNRAENQYRHIMVFDSTDPEKKMKISQISKPLADHFNPFVIMDGEKKRIGYHRCGAQIERQFSKIKSPLEDVGLFRLSGAFPTFSNDGKRVAFVDNEFKSVWVADDKGLRITEGANKIFAPVWNQKEDILYVCRGPSFSDKETLDIYRIPNVSTARQHAEQLTDGSNNAFPSTNKDGTKLVFRSTRDFKDSEKKYKNLYIMEDAESGDFGEGEITRLTNGHWIDTHCHWSPSGDWIVFSSTRDKPASAPESDNGLDAGYFAIYLVNPKDPSVVVRVLGSVPATDLTNPLAGHVNHPIFSPDGKSIVVVADLAAVSVDPISLPLVEHSVRPYGDIFTVDIDKDDIKKNQDLTKYERITHTRYENSTASWTMFSTEDPNAAWNLQFSGKYSPACPYAPADGRESWHMTGHLCIPKRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0XY03_DAUCS</name>
    <protein>
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            <fullName evidence="5">Aspartic peptidase DDI1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
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            <tissue evidence="9">Leaf</tissue>
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    </reference>
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        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
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        </subcellularLocation>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AI31</accession>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Zheng Z."/>
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            <tissue evidence="24">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        </reaction>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000687">
        <property type="entry name" value="Leucine Rich Repeat family protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000453">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000356">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
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    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
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        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042210373" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="27"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="859"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="924"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="952"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="WOG81212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1229" mass="134751" checksum="763BF96A5D902861" modified="2024-05-29" version="1">MGTFQKPLFVLVHILSLSLFFLNVTASPRTEAQALVQWKESFSSSSLDSWSLSNFRNFCKWTGISCNSAGSVSRINLHDANITGNVALFSFSSFPDLTRLDISSNHFDGPLPSIGTRFSMITYLDLSANTFEGGIPPEIGNLKELRYLSFYDNSLNGTIPYQVSKLQKLWYLDLGSNYLATPDWSKFAAMPRLTHLSFFYNVLDSEFPDFLLNCRNLTYLDLSSNLLTGPIPEMVFSNLDNLEYLNLTDNSFRGALSANISKISKLKDLRLGRNQFSGFIPNAISLLSDLQILELYNNSFEGNIPSSVGQLSELQQLDLRINKLNSTVPPELGKCNKLTYLALAGNSFTGPLPLSFSNLTKLSELGLSENSLSGELSPDLISNWTELTSLQLQNNHLTGYIPPAIGKLTNLSYLFLYGNSLSGPIPSEIGNLAYLVALDLSANQLTGPIPPTIGNLANIGVINLFSNKLSGTIPPEIGNLTTLVTLDLNTNQLNGELPNTIADLQNLETLSLFTNKFSGSIPSDLGKNSLFLANVSFSNNSFSGELPKGLCSSFLFSEFTVNRNNFTGPLPDCLKNCSGLTRVRLERNQFYGNISEAFGVHPNLVFMSLSWNKFTGELSSKWGKFEMLTNLQMSGNKISGQIPPELGNLTQLGVLQLDSNALTGDIPSELGQLSRLFNLNLSNNHLSGEVPQSLGNLTKLQYLDLSTNKLRGSIPKVFGNCVNLLSLNLSHNSLIEAIPSELGSLSHLQYVLDLSSNSFTGIIPPSLGKLSSLENLNLSHNNLSGRIPNALSDMISLQFIDVSYNNLSGPIPSGKVFEQALANAFTGNKGLCGNATGLPSCSGTTKTAKAEKSSNKVRIIVIVPVVSILVLAIIIALYVVFYKKVKQHNDDSKSSDITDNSESLIWGKEGRCTFRDIIKATDDFDEKYCIGRGGFGSVYKAVLLTGETVAVKRIGMPQSTDVPAINRWSFENEIRTLTEVRHRNIIKLYGSCSKLGCLYLVYEYMEKGSIGNLLYSEDGAFELGWDTRLRIIQGLGHALSYLHHDCTPPIVHRDVSPNNILLESDLEPRLSDFGTARLLTTDSTNWTSVAGSYGYMAPELAFSMRVTEKCDVYSFGVVALEIMMGKHPGELISSLSTKTMSENDLDFKDVLDQRILPPTGTLADEVMFAVNAAVACTHSTPDSRPTMRFVAQELSARIQTREPEKFGQIKLSKSPSLQRVSHGIFLDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIU0</accession>
    <name>A0AAF1AIU0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0101181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AIU0"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250:SF17">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21021" checksum="C4E0C9943EC309D3" modified="2024-05-29" version="1">MASDMESNNSSVERGAGKNKRKWTEDEDGKLVEALMKTLKKDFDVVYDICYGANSSGFGWNAEDNVLTAPRDVWVQYLNTHSDESTSKKRKIGNVESLLEAVYAASDRISNQFEASTKLLIATEEDMMQKKKQLNDELSKIPNLTVLQKLQVPKKIAKDEDLMILFFEAPTEEKIILVNAILNNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJG9</accession>
    <name>A0AAF1AJG9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">EGF-like calcium-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">DCAR_0101651</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasmic vesicle</location>
            <location evidence="16">Clathrin-coated vesicle membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Prevacuolar compartment membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJG9"/>
    <dbReference type="KEGG" id="dcr:108204517"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02125">
        <property type="entry name" value="PA_VSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000001">
        <property type="entry name" value="Vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041939972" description="EGF-like calcium-binding domain-containing protein" evidence="19">
        <location>
            <begin position="23"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="20">
        <location>
            <begin position="515"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="WOG82486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="626" mass="69054" checksum="BF5803681E8F23F9" modified="2024-05-29" version="1">MGLKIKLFLGFMVLNFCESVMGKFVVEKNSFRVTSPDSLKGTHDSAIGNFGIPQYGGSMAGTVVYPKDNKKGCKSFDDFGISFKSKPGSLPTFVLVDRGDCFFALKVWNVQNAGASAVLVVDDTEEPLITMDTPEEDVKAAKYIQNITIPSALLDKSFGEKLKKVISNGDMVNVNLDWRESVPHPDDRVEYELWTNSNDECGIKCDMLMDFVKDFKGAAQLLERGGYTQFTPHYITWYCPMAFTISKQCKSQCINHGRYCAPDPEQDFSSGYDGKDVVIENLRQLCVFKVANESQTPWVWWDYVTDFQIRCPMKEKKYNKECADSVIKSLGLDSKKIEKCMGDPNADSDNPVLKEEQDAQVGKGTRGDVTILPTLVVNNRQYRGKLAKGAVLKALCSGFEETTEPAVCLSGDVETNECMEKNGGCWEDKASNITACKDTFRGRVCECPVVDGVQFKGDGYSSCVASGPGRCKVNNGGCWHETKGGHTFSACSDKGDGKCVCPPGFKGDGVKSCVDVDECKDKKACQCPECSCKNTWGSYECTCSGDNLYIREHDTCISTTAAEGKSAWTAVWVILIGLAMAAGGAYMVYKYRLRSYMDSEIRAIMAQYMPLDSQNEIPNHVSDDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKF3</accession>
    <name>A0AAF1AKF3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0102910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF90">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="1">
        <location>
            <begin position="212"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG83732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1426" mass="163289" checksum="748AB8A8020D04D2" modified="2024-05-29" version="1">MYNNAFGFTSVGANIDKSINNGRGPFVYRIHGVIYHQIGSLFAEESKKPVFSQIYMYDNLEQVNKRMNFPNSEEPLDSEITELLSVMLHRVNALVDIYRQVRDRYKESEVIPGKLRLIANRDTDGRESNVPTNAYDFAGLVANSNLADDRDILVHPHDGVLHRISVLHPCYMSLQYPLLFPWGEDGFRIDILHSGISAKSGNKRDVVTIREYYCYRLQYRDAEGHTLIGGGRLFLQFVVDAWACIEHTRLTWVLTHQTILRSDLYNNVVDSVNKGDTNASTIGKRIVLPSSFTGSPRYMQQNYQDCMAICRKFGSPDLFITFTCNPQWPEIKEYCDKVPHCIPADRPDVMARVFKIKLELLLEDLTDNHVLGKVIGVAYTIEFQKRGLPHAHIIVWLEESDKCHSTDDIDQLICAEIPDKESDEIGYDAVSRHMIHGPCGAYNIDCPCMKDGKCTKYYPKEFTNETTIDSNGYPVYRRRDDKHTVHFKEKDIEIDNRFLFYISVIEYNCMGCNSTYLTYFYCISISRFVVPYNRGLLVKYQAHINIEWCNQGRLIKYMFKYVNKGPDRATVVVETADAVVTNATKQYVNINEVEEYVSCRYLSSAEACWRIFEFPIHHHKPVVIKLVFHLENEQQVYFREDETLTTVVDRIDPNATMFIQWFRVNREDPAARELTFVEFPEKYFWDNTGKIWQRRKKKMCVIGRMVYVHPTAGERFYLRMLLNIVKGATSFEDVRTVNGVVYNTYKEACFHHGLLECDDEWHTAITDASTHQTGAQLRELFVTLLLFCDISDVRALWDKHWKSFSDDIEHRQRKHFPNRAFVINDEQLESLTLYDVDLQLRKRGKTLADFPTLPKLDRDLQRQSTNTLLYEENMYDRHALAQVPYADWVVSVGDGVVPTVPSVEGNEPCWIEIPSELNLDPGDDGKKAIIDAVYDELCNSSNDSGYFRDRAILTPLNEDVDLINKEVMKRFTGQSKIYRSVDSVCKSTVNYESMESMYTPEYLNSLRIAGVPNHQLELKIGAPIVLLRNLAPSKGLCNGTRLIVTQLCARVIEAIIVTGNHIGEKAFIPRICMRPSDTALPFTLKRVQFPISLCYAMTINKSQGQTLKYVGLYLPRPVFSHGQFYVAISRVTTPLGLHIVCENETHPIVGMTKNVVYHEQISSNRCIVIVDQHMPQPKPISLLMKRYALLIIIYMCGTQVQAGPLQFGLIADFSKRLLLGSVYLISNYDVAAAPDTYRPVPGEYSVNFHRKTSVKKIGDVPAIPMFQFNLKTFEETRARLGDVVTLIDVVGKLKDYTHIQTAKSGKKSLDIVLADKSQEQQNMANVEAVTIAQLFEAQLPDGKNFIEFYTEATVIGLFPNEGWYYIGCNKCGKKMNDFGQCIKLTLAVKDSTADTSFVIFDRHVMKLINVSAQHLLNSDQVFTFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AKQ8</accession>
    <name>A0AAF1AKQ8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0205449</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Apiaceae</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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            <tissue evidence="10">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant may improve abiotic stress tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        </reaction>
    </comment>
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            <name>a divalent metal cation</name>
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    </comment>
    <comment type="pathway">
        <text evidence="3 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
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        <text evidence="4 8">Belongs to the trehalose phosphatase family.</text>
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    </dbReference>
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</entry>
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                <person name="Senalik D."/>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="22"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 second beta-propeller" evidence="3">
        <location>
            <begin position="497"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 TPR" evidence="4">
        <location>
            <begin position="924"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
            <begin position="668"/>
            <end position="704"/>
        </location>
    </feature>
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        <location>
            <begin position="1277"/>
            <end position="1301"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
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        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF23751"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF23752"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF23753"/>
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            <dbReference type="EMBL" id="WOG92477.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1333" mass="146506" checksum="F3C0E9B87DCC2E6C" modified="2024-05-29" version="1">MSSSSTSPRSTSESGDFILPSPPSRHNGGAADLSPTGLLAYAASTSVSIVDSRSMQLISVIPIPPPPSTPISSFITSVRWSPQPLRRDILAHDPSTSLSHLLLAAGDRYGGIALLDLRIKAPLFFLETPDYIHANKPGIQDLCWIQARVDSWILASLSGPSLLSLYNTSTGRCFFKYDAAPELFACVRRDPFDSRHFCALGLKGFLLSAKAHSDGSEEDIQLNELRINTDSSEIQRLEKDAYTNPAAAMSPPLAIFPTYVARVAFSPHWRHILFLTFPKELLVFDLKYEVALFVTSLPRGVGKFIDVLPDPSTELLHCAHLDGKFSTWRRKEGQQVHTMCMTEELMPLIGSSVPSPTLLAVTLSSTESVMQNFGKHCSNVLNTVPVLDIKHHFEVNDSSIIICNTHLIAISDDGKIWDWLLTAEGNTESLKEKLNSTNMSEANDVNVSRSNIEVASTHDSTLVHPEDTRRCTTRFSIHCVTQEKITFKINLVGQLHLLSSTITVLAVPTPSLTAIVARGGNYPAVAVPLVALGSQSGTIEVIDVSANSVAASFYVHNNVVRGLRWLGNSRLVSYSYTLANEKSGGYINKLVVTSLKNGQNRPFRVLHKSERAPVRALKASSSGRYLLIMFRDAPVEVWAMTKTPLLLRSLALPFTVIDWTLPTVPRPGQTAQSRHLPVVSEDSATVAQMGATSPRTSTDSLEGSQDESAESFAFALVNGALGVFEVHGRRIRDFRPKWPSSSFVSTDGLVTALCYRLPHVVTGDKLGNVRWWDVTTGQSSSFNTQREGIKRIKFSPVAPGDRSRGRIAVLYFDNTFAVFDLDSQDPLASSLLQPQFPGILVLELEWLPLRTDRNDPLVLCIAGADGTFRLTEVNISDKKGGYVPHPRFVKERFRPVPLCSPVLLPTPHALALRMILQLGVKRSWFDAATNVDQGNSKLTTTTTTDDLRSYLIDLPPVVGDSVVPELLLKVLEPYRKEGGILDDERVNIYTTLVDKGSIMRFAFAAVIFGEYPEALFWLLLPRALKHWITKIVSKSHQKAQVSAPTSDVNNASMLVMLASKEKAASENKNVVMNFGQLKQMAFEHGELWEMANERITWHEKLEGEQAVQKQIHELVALGNLEAAVTLMLSTPPESSYFPSNALRAVALSSAVSKSLFELSVNVVAANMVRTDRSFSGTHLLSAVGRYQEACSQLQDAGCWTDASTLAATHLKGSAYARVLQRWADHVWHVEHNIWRAMILYVAAGAFPEALVALREAQQPEAAAMFIVACHEIQKAFTSDSESHDESGPAAKEPVPPLPGLNSDNEDVIAVGEYYGEYQRKLVHLCMDSPPLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATY9</accession>
    <name>A0AAF1ATY9_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0414605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS01G0541250 PROTEIN"/>
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    </dbReference>
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            <dbReference type="EMBL" id="WOG95293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38082" checksum="73A8A2D7762BE283" modified="2024-05-29" version="1">MQKAAGQPYNMTTKGHNSGIKRIQVQPNAITEINMGDRACIRDLAVQLKVSSSTANRMIKKGLIKPHTNPLHPALKEANLFQRFEWVLNLLMGDTPTTKRQYYPIYNFVHIDEKWFYLSKKSHRVYLERNVKGKYRAAKSSKFIPKVMFTAAVARPRFNADKECTFDGKIGIFPFTYQEPAEKASKYRAKGTIMTKIVESDNARTHIGQDDPEWQQAHQQGHFTFILVQQPPNSPDLNILDLGFFRSIQFLMHKKVPKDVDDMLEAVHHAYNELEPTTLGNVWLSYKYVMNEVLKVKGSNDYLVPHVNKKKLAAEGKLPIQVTAPMWVVHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AU84</accession>
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        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="8" type="ORF">DCAR_0311961</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FYVE_scVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="258" mass="27951" checksum="15826280C37730E9" modified="2024-05-29" version="1">MSLEPPPFQEASRCDVCKCSFNTFRRRHHCRCCGRTLCAEHSSTQMALPQFGLQSLVRVCSDCYNDASRKKDDTQASLSTVNNVSDSISRLDIRAVAEPESKLPIKQLDVLECTCGMPLCICEAPAPAPVPIPARDSGVTSSSTFQSNTKPKKSDSKPRSRGSASNSNLGPASNSSSNSLESNKLYEVNGEGVDANYRDKQGLSLLHLAAVFNQSDIAFVLMDHGASITCKNSQGETPLDCAPITLQNKMKKKIEQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX78</accession>
    <name>A0AAF1AX78_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0415012</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Iovene M."/>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH10879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="370" mass="39367" checksum="91635769CE5DA0D2" modified="2024-05-29" version="1">MSTMSQLHRLSLPNHYHIFNTNRSIDLIHSNLAFSSPPTCLKLFNSPLPKNQLTHYYLITKVHIPCANIAAAGGSNNDGIGGFGGGGSDGTSGNNDGDDSSSSSNFERFGPVGAFLNGWRSRVAADPQFPFKVLMEEIVGLSACVLGDMASRPNFGLNELDLVFSTLVVCFILNFTLMYILAPTTASSGVALPALFASCPTSHMFEPGPFSVLDRFGTFVYKGIVFAAVGFTAGLVGTALSNGLLALRKNMDPTFETPNEAPRTMLNALTWAAQVGLSANLRYQTLNGVEYVLAKGLPSFAFKTSVIVLRVVNNVMGGMSFVILARLTGSQSSGANESKLVSSEEVEASATGDEKDKLPQDSESSESVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="42"/>
        </location>
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        <location>
            <begin position="126"/>
            <end position="166"/>
        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH08737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="180" mass="20902" checksum="2007245CBCBF55C4" modified="2024-05-29" version="1">MQNSNSRSLQKYKFEVKMIFLAFVMNFQTFFGIKTAKIIWGYKYPAYTFIDTVNNNKIFIKSFRNLSRKSSFHSDHQFVDDKQCSYSIWCGRIFHMQELLDLGETVGTQSRGLSQENISLLPISKCVICQMEYKRGDQQINLPCKHIYHSGCGSRWLSINKACPICYKEVFANASKQSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BA88</accession>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
            <position position="138"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH09021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="333" mass="37575" checksum="8FF7D8E9F316CAE9" modified="2024-05-29" version="1">MIIKRSCSSSLIVGLLSNHQRCCWSCSMAANSSSALVRYSYKDAFELDNCNGFQSLALKFKPFSRTVCVKSCGDKINELGNDKLLFKGRRPRAFSQFIGGGSGKKSHGLVDQVFDNTGGCHTLGFPDPPKLVVAVDVDEVLGNFVSALNRFIADRYSSNHSVSEYHVYEFFKIWNCSRKEADIRVHEFFKTSYFKKGIHPIPGAQQALQKLSRFCNLSVVTSRQNVIKDHTVEWIERHFPGLFHEIHFGNHFALDGQSRPKSDICRSLGAKVLIDDNPRYAAECAEAGIKVLLFDYDNTYPWCKVESVAQHPLVVKVHNWEEVERQLVSQVVS</sequence>
</entry>
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            <fullName evidence="3">Sulfotransferase</fullName>
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    </gene>
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            <taxon>Viridiplantae</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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        </lineage>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108200666"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X3X2"/>
    <dbReference type="Gramene" id="KZM80319">
        <property type="protein sequence ID" value="KZM80319"/>
        <property type="gene ID" value="DCAR_031801"/>
    </dbReference>
    <dbReference type="Gramene" id="KZM87143">
        <property type="protein sequence ID" value="KZM87143"/>
        <property type="gene ID" value="DCAR_024277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044987">
        <property type="entry name" value="AtMg00030-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37773">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37773:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="15343" checksum="710D9F671AB99A40" modified="2016-07-06" version="1">MKPGEKAVNTLLRNEASCLFFFKPPIRSRSPLLTESRLISLPLATQMFQFAKFEKSKERRLATELGYGFPIGDPWITDGISPWPFASESVLPSQCPGIHPMHSFRSCTLCRLASHRLHKRYTEHQPNIDSKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YG29</accession>
    <name>A0A161YG29_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0625669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YG29"/>
    <dbReference type="Gramene" id="KZM91949">
        <property type="protein sequence ID" value="KZM91949"/>
        <property type="gene ID" value="DCAR_020686"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228088"/>
    <dbReference type="OMA" id="CTSNDEP"/>
    <dbReference type="OrthoDB" id="733571at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31182">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31182:SF15">
        <property type="entry name" value="F26K24.5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="745"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="750"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="778"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH06246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="801" mass="89637" checksum="8C21A5749ECE7C56" modified="2016-07-06" version="1">MVVKMMKWRPWPPLQSKKLEVKLVVKRLDGLSPDEKDGQKMGVEIRWKGPKIGLGSFRRTVKKNVTKEVGFDQNGVVLWDEEFLSLCALSGYKDDVFHPWEISFTVLNGLNQRLKNKVPNIGTASLNLADFASKAEGKEFELSIPLTVSGTAAEPHPSLTISLGILELRAAQEPAESVQRPIVRSPSPQRPRGNSLSEKDELSALKAGLRKVKIFTEYVSARKAKKACREEDGSEGRCSARSEEGDYTYPFDSDSLDEFEEGESDEIKEDSSVRKSFSYGTLAYANCAGQTINSSTRSNLEDEGWVYYSNRKSDLACSNIEDSTASISETVTHNSKRSLLPWRKRKLSFRSPKVKGEPLLKKDYGEEGGDDIDFDRRQLSSDESLSHGSKTDEDSSAHRSSVSEFGEDNFAIGRWEQKEVTSRDGHMKLQTQAFFASIDQRSERAAGESACTALVAVIADWFQNNQKLMPIKSQFDALIREGSLEWRNLCEDESHRDRFPDKHFDLETVLQAKIRSLFVVPEKSFIGFFHPDEMEEGRFDFLHGAMSFDNMWDEISRAGSECSTRGESLIYIVSWNDHFFVLKVDPEAYYIIDTLGERLYEGCDQAYILKFDRNTTICKLPTDATKPVEEKPTDKQIVVAEAELRNQQANQVDCKEHSVDGAVYEPDVLVKSDNEEVVVCQGKDSCKEYIKSFLAAIPIRELQADIKKGLMASTPLHQRLQIEIHYTQLQVPAPKSSDELAMVTTTTNGSSSPVSELTSSSSPVSELAMTTTRTTADSSPVSEFTSSSPVPEVAIEEAAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YJC6</accession>
    <name>A0A161YJC6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YJC6"/>
    <dbReference type="Gramene" id="KZM93389">
        <property type="protein sequence ID" value="KZM93389"/>
        <property type="gene ID" value="DCAR_016634"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221228"/>
    <dbReference type="OMA" id="YMINEEV"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08603">
        <property type="entry name" value="GDPD_SHV3_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08604">
        <property type="entry name" value="GDPD_SHV3_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000011">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000013">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF7">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG99656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="760" mass="83432" checksum="A22FBB9D4DA6CD3B" modified="2016-07-06" version="1">MFKASIMLLCLIVFAAHAHHAKRSTSNNNTSSLWKTLTGDAPLAIARGGFSGIFPDSSIDAYEFALLTGLPNIILWCDVQLTSDGAGICFPELTLDNGSDISSLFDRRKKSYLVNGATRTGWFSVDFTLDDLTNISLTQGIYSRTNMFDQNFFQVVTVEDVARQLKRPGLWLSIQHDAFFSQHNLSMKSFVISASRSGIVNYISSPEVNFLRSIVTQFKESPTKLIFHFLGQNDIEPTTNQTYNSLLINLTYIKTFASGILVPKTYIWPLDRDLYLRPHTSVVLDAHSKGLEVFASDFSNDVPFAYDYNYDPVAECLNFIDNGNFSVDGMLSDFPITPSEAIDCYSHMDNNLSRAAIPLVIAHEGSSGVFPGCTDLAYKQAISDGADVLDCPVQMTKDGTPFCLGSINLIHGTTAAQNFSNLIMNIPELDGNGIFSFSLDWSDIQRLQPMISNPHQGASLYRNPRNKNAGNFVTLSDFLALANNADSVLGVLIHIENASYLAEKEGLSVTDAVVDALSKAGYNNQTRKKVMIQSSSSAVLVKLKEEKNNYEFVYEIEEDIRDTLNSTILDIKKFANSLVINKNSVYTQNLKFLTGATGVVSKLQAFNLSVYVELFQNEFLSQAWDCFSDASVELNLFIVGSGVDGVITDFPGTANRYRRNRCLNLGKKTPRYMMPVAVGPGDLLSVSETQPPAEAPNPVLTVSDVTEAPLPAIVAKPHTDSGTRSEATAPLPKQPSGQVKVGSGCFMLNLVVLLITLILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A1G2</accession>
    <name>A0A162A1G2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF868 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_016925</name>
        <name evidence="2" type="ORF">DCAR_0519353</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="301" mass="34363" checksum="55D9AE35F7F94880" modified="2016-07-06" version="1">MRSIATCYSEHAIKVSNSYCSGPSNHATFSPNMIPSIQDAVTCIYRVKLCSQHQLLISLTWCSSLMDRGFTVTLVQDPSSPFKFHTRRLYLHNIKGTKSFKSTNSKVEIFWDLSFAKYDAGPEPVRGFYIAVFVNYELSLLLGDMEQEPEVKKLMCNVPMSKFSFVSRSEHFSGGAACSTKAQFFTTGTRHDILIKCIVQEKGQKNSILSVSIDDKNVVQVKRLQWNFRGNQIIFIDGCLVDMMWDVHDWFFNPKSGYAIFMFRPRSGMESRLWLEESKVEQNEEEKAGFTLLISACKNPD</sequence>
</entry>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
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                <person name="Senalik D."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
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        </cofactor>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2">Single-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="435"/>
        </location>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000021">
        <property type="entry name" value="DUF21 domain-containing protein At4g14240-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="29"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM95170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH01674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="493" mass="53301" checksum="42E5BAEF86347E7F" modified="2016-07-06" version="1">MTLINTVTLATVLATHRSFGSSADNIEFGSVWWFVYAGISCFLVLFAGIMSGLTLGLMSLGLVELEILQRSGTPTEKKQAAAIFPVVQKQHQLLVTLLLCNAASMEALPIYLDRIFHPVVAVLLSVTFVLVFGEIIPQAICTRYGLAVGANFVWLVRSLMFICYPIAYPIGKVLDAVLGHNDALFRRAQLKALVSIHGQAAGKGGELTHDETTIISGALDLTLKTAEEAMTPIESTFSLDVNSKIDWEAIGKILARGHSRVPVYSGNPKNIIGLLLVKSLLTVRAETETPVSAVSIRRVPRVPADMPLYDILNEFQKGSSHMAAVVKVKTQNTVSDCEVEKAFGNKVTKGKYQLTVPLLSEHDEESDNHVVDVDNASNTTALKHTFISQSEAATTNVAENIEDGEIIGIITLEDVFEELLQEEIVDETDVYVDVHKRIRVAAAVAASSVARAPSSRKLIAQKVVGGQGKQGDSPHLQSRSPGTTKGSLFSTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AB54</accession>
    <name>A0A162AB54_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_014388</name>
        <name evidence="14" type="ORF">DCAR_0416541</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM98250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AB54"/>
    <dbReference type="STRING" id="79200.A0A162AB54"/>
    <dbReference type="Gramene" id="KZM98250">
        <property type="protein sequence ID" value="KZM98250"/>
        <property type="gene ID" value="DCAR_014388"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219296"/>
    <dbReference type="OMA" id="SGLEGYW"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042651">
        <property type="term" value="C:thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010277">
        <property type="term" value="F:chlorophyllide a oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd04337">
        <property type="entry name" value="Rieske_RO_Alpha_Cao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015881">
        <property type="entry name" value="ARHD_Rieske_2Fe_2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013626">
        <property type="entry name" value="PaO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF19">
        <property type="entry name" value="CHLOROPHYLLIDE A OXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08417">
        <property type="entry name" value="PaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00570">
        <property type="entry name" value="RING_HYDROXYL_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Rieske" evidence="12">
        <location>
            <begin position="218"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KZM98250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="533" mass="60039" checksum="82A8EB537E458AF9" modified="2016-07-06" version="1">MCSIATAAALSLPISFSPYSKSISKRGFKAGFRVFAILGDENGVVDKKNPWGAIFEVEQPRFKVTHVRGKFVDVNEALEVARFDIQYCDWRARQDLLTIMLLHEKVVDVLNPLAREYKSIGTVKKELAELQQELAQAHRQVHISEARVGAAVDKLAYMEELVNDRLLQDRSVTETDGISPSPSTSAESLETVKKKMPRKSLNVAGPVQPYHSRLKNFWYPVAFTSDLKDDTMIPIDCFEQPWVLFRGKDGKPGCIKNTCAHRACPLDLGSINEGRIQCPYHGWEYATDGTCEKMPSTKFVNVKLKALPCFEQEGMIWIWPGSDPPAATIPSLQPPPGFKIHAEIVMELPVEHGLLLDNLLDLAHAPFTHTSTFAKGWSVPSLVKFLTPASGLQGYWDPYPIDMEFKPPCIVLSTIGISKPGKLEGQSTSQCATHLHQLHVCLPSSKNKTRLLYRMSLDFAPILQYVPFIDHLWKHFAEKVLNEDLRLVLGQQDRMINGENVWNMPVSYDKLGVRYRLWRDAVERGVKQLPFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ADU7</accession>
    <name>A0A162ADU7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_013238</name>
        <name evidence="15" type="ORF">DCAR_0417837</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the early nodulin-like (ENODL) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ADU7"/>
    <dbReference type="STRING" id="79200.A0A162ADU7"/>
    <dbReference type="Gramene" id="KZM99400">
        <property type="protein sequence ID" value="KZM99400"/>
        <property type="gene ID" value="DCAR_013238"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217477"/>
    <dbReference type="OMA" id="PADYLNC"/>
    <dbReference type="OrthoDB" id="959565at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11019">
        <property type="entry name" value="OsENODL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000010">
        <property type="entry name" value="Early nodulin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041846">
        <property type="entry name" value="ENL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF14">
        <property type="entry name" value="OS01G0272700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007832059" description="Phytocyanin domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="13">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZM99400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG98494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="20954" checksum="ED6D88FC68DD93AB" modified="2016-07-06" version="1">MALSKLFFILNIAIFVSSVFSYQFEVGGDEGWITPSSANFTTDIDEPYNVWASHNRFRIGDTLHFRYENDSVLVVGYEDYKNCTTTNAISEFDNGDTVFKFKRSGFFYFISGQPDHCTSGQKMIIRVMHPSEYTPPHMQPQSPESAPSPGTRALGPSGDSYSSSTRVSAVAAFVVAALAAPLVIIYLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SM72</accession>
    <name>A0A164SM72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="7">3.4.21.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_023458</name>
        <name evidence="10" type="ORF">DCAR_0726910</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="7"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SM72"/>
    <dbReference type="STRING" id="79200.A0A164SM72"/>
    <dbReference type="Gramene" id="KZM86324">
        <property type="protein sequence ID" value="KZM86324"/>
        <property type="gene ID" value="DCAR_023458"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193814"/>
    <dbReference type="OMA" id="GIFDTMQ"/>
    <dbReference type="OrthoDB" id="2017408at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009840">
        <property type="term" value="C:chloroplastic endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000001">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001368">
        <property type="entry name" value="PRK00277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009205">
        <property type="entry name" value="PRK12553.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF11">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZM86324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH07480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="243" mass="26835" checksum="F57DEF0FE32550F4" modified="2016-07-06" version="1">MRSLISRSLCHVSAGIGKTITGGHTSTFTHRRCYIPMVIEHSSRGERAYDIFSRLLKERIVYINGPISDDTSHVVVAQLLFLESENPSKPIHMYINSPGGAVTAGLAIYDTMQYIRSPISTICIGQAASMGSLLLAAGAKGERRSLPNARVMIHQPSGGYSGQAKDITIHTKEIVRIWDALNTLYVKHTGQPIEVIQKNMDRDYFMTPEEAKEFGIIDEVVDERPMALVTDAVRTEGKDKTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TAG4</accession>
    <name>A0A164TAG4_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0728211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH08933.1"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
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            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U216"/>
    <dbReference type="Gramene" id="KZM88331">
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        <property type="gene ID" value="DCAR_025406"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193229"/>
    <dbReference type="OMA" id="ACPWCNV"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46616:SF2">
        <property type="entry name" value="OS03G0211100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46616">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH09704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="254" mass="28810" checksum="1626F16ECB48BF91" modified="2016-07-06" version="1">MWNFASNCVAGNNGLKNDSHKHRTDALDSSDDELSSCTSREEGLECPICWESFNIVENVPYVLWCGHTLCKNCILGLQWAVVKFPTIPIQLPLFISCPWCNLLSVRLIYRGNLKFPRKNYFLLWMVESRNGDRGKSSSVYPGDNNHGGWPANTELESEEGHHPESSRSNRNVDSYLNARSLSSSLQKSLIFIVNLTTKFPLVLMFLLIVLYVIPASAAVILLYLLITVLFAIPSFLILYFSYPSLDWLLKEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VX71</accession>
    <name>A0A164VX71_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_021649</name>
        <name evidence="13" type="ORF">DCAR_0624574</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VX71"/>
    <dbReference type="SMR" id="A0A164VX71"/>
    <dbReference type="Gramene" id="KZM90986">
        <property type="protein sequence ID" value="KZM90986"/>
        <property type="gene ID" value="DCAR_021649"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225540"/>
    <dbReference type="OMA" id="NCYLLCW"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF225">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZM90986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH05160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="271" mass="30766" checksum="1339AF5DCE625E88" modified="2016-07-06" version="1">MALFHFANPELFAFGILGNLVSFLVYLAPIPTFYRIVKKKSTEGFHSIPYVIALYSATLMIYYALNKNDATLLITINSFGIVIETIYLSLFIAYAPKKIRVSTIKLVVLLNIVSYVMIVAVTYFFIQPPKRVHILGGINLLLSFIVFVAPLSIIKKVVQTRSVEFMPFWLSFFLSLSAVMWFFYGLLSKDIYVALPNILGFTFGIIQMVLYGIYKNCESKPVEELKLPEIVKGTPEIYPVSSFPSSENDDDEEKKKDENIKDKNMDASDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164WNG5</accession>
    <name>A0A164WNG5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetylglutamate kinase</fullName>
            <ecNumber evidence="2">2.7.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_020609</name>
        <name evidence="12" type="ORF">DCAR_0625749</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM92026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164WNG5"/>
    <dbReference type="STRING" id="79200.A0A164WNG5"/>
    <dbReference type="Gramene" id="KZM92026">
        <property type="protein sequence ID" value="KZM92026"/>
        <property type="gene ID" value="DCAR_020609"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224333"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="438291at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000004">
        <property type="entry name" value="Acetylglutamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="10">
        <location>
            <begin position="78"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZM92026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH06324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="339" mass="35496" checksum="A777CE737A0FC288" modified="2016-07-06" version="1">MAAKALHLPKSTPLLNPPPKPLINPNPNHLHFPKSPISLPPLKSASISQFDPILATSPSTRVRILSEALPFIQKFRNKTIVVKYGGAAMKSGNLQASVIADLVLLSCVGMRIVFVHGGGPEINLWLAKLGIKPNFLNGLRVTDASTMEIVSMVLIGKLNKMLVSLINKAGGTAVGLSGIDGRLLTASPAVNAEELGFVGDIAEVDSSVIRPLIDNGCIPVIASVAADGNGQMYNINADTVAGELAAALGAEKLILLTDVVGILEDRNDLGSLVKEIDIKGVKKMMEEGKIAGGMIPKVNCCIRSLAQGVRTTSIIDGGLEHSLLLEILTDEGAGTMITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XFR9</accession>
    <name>A0A164XFR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0518723</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XFR9"/>
    <dbReference type="SMR" id="A0A164XFR9"/>
    <dbReference type="Gramene" id="KZM93121">
        <property type="protein sequence ID" value="KZM93121"/>
        <property type="gene ID" value="DCAR_016366"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220841"/>
    <dbReference type="OMA" id="YMIYKKP"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF22">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG99375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="294" mass="32463" checksum="8CA43BAC378FD6C8" modified="2016-07-06" version="1">MAPLSPHWTLAFGLLGNVVSFMVFLAPIPTFYKVYKKKSTEGFQSVPYVVGLFSAMLWIYYALLKSNAFLLITINSVGCVIQTIYIVIFLCYAPKNAKLQTIKLLVGMNVVGFGMIIGLTQFVANGVASRVTIVGWICLVFSLCVFVAPLGVVRQVIRTKSVEYMPFLLSLFLTLSAVMWFFYGLLLKDINIAVPNVLGFSFGVIQMVLYMMYKDAQKINTKSEKLPEFQDQIIVVDERKFPELAEQIVDVMKLSALVCSEMKPVAPPTPCGNETPVVVAVPPPALPVQPVAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XHA5</accession>
    <name>A0A164XHA5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_016421</name>
        <name evidence="2" type="ORF">DCAR_0518776</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XHA5"/>
    <dbReference type="Gramene" id="KZM93176">
        <property type="protein sequence ID" value="KZM93176"/>
        <property type="gene ID" value="DCAR_016421"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM93176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG99428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="70" mass="7613" checksum="3E20C4462AD976E5" modified="2016-07-06" version="1">MTEVPPSVLEDNAIGNKGFYLYPLGTSNNAEPQLLDLYVSHISGTQAPELVSYISRFLNSYGHGQNVDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZIH2</accession>
    <name>A0A164ZIH2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_019218</name>
        <name evidence="3" type="ORF">DCAR_0521998</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZIH2"/>
    <dbReference type="Gramene" id="KZM95976">
        <property type="protein sequence ID" value="KZM95976"/>
        <property type="gene ID" value="DCAR_019218"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221803"/>
    <dbReference type="OMA" id="LCRNIHF"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000022">
        <property type="entry name" value="F-box protein FBW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF80">
        <property type="entry name" value="F-BOX DOMAIN, LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM95976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="305" mass="34794" checksum="35446FE829DE4A91" modified="2016-07-06" version="1">MDSADRRGWDGLIPDVLGSIFSKLSLEDVLTVVPSVCKSWHKVVMGPYCWQSIDIEQWSDQQSHLDRIERMLRMLITRSNGSMTKLSVSRLQYDHLFSFIADNSRSLQILRLTRSKISDSIVEQLADRLSGLTFLDLSYCDKIGARALEAIGKQCKFLGGLCLNMHPIDVLGKPSQKDEARAIATTMTKLKHLELTYIHLDTTNVVEIISNCPNLEFIDLRGCWDVDLDNSWLKDEFSKLKVLGPHVADHLEIHCCWECEDSSYYSDSVYDYESWDEFDDDDGTLEIILYEGQGENSDNAWPQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165X9R1</accession>
    <name>A0A165X9R1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_014682</name>
        <name evidence="2" type="ORF">DCAR_0416217</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165X9R1"/>
    <dbReference type="Gramene" id="KZM97956">
        <property type="protein sequence ID" value="KZM97956"/>
        <property type="gene ID" value="DCAR_014682"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053115">
        <property type="entry name" value="CDK_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35162">
        <property type="entry name" value="OS08G0516600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35162:SF2">
        <property type="entry name" value="OS08G0516600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM97956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG96879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="73" mass="8278" checksum="711CECF03AFA0FF5" modified="2016-07-06" version="1">MQNANPIHKAEEFECVTPKSDRLVKCSTSVCPPAPKKLKQRNKFAVTALTERPHQVYVQVPKDLSSIFLLRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YXG7</accession>
    <name>A0A165YXG7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0417783</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YXG7"/>
    <dbReference type="Gramene" id="KZM99357">
        <property type="protein sequence ID" value="KZM99357"/>
        <property type="gene ID" value="DCAR_013281"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108218383"/>
    <dbReference type="OMA" id="RFGCCSN"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG98440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="217" mass="24355" checksum="370963AC5C435F73" modified="2016-07-06" version="1">MSAYRTEDEYDYLFKLVIIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATKTLDFDSKVIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDVTRRNSFENVERWLKELRDHTDQNIVVMLVGNKSDLRHLVAVSTEDAKDLAERESLYFMETSALEATNVEKAFKEVLTQIYRVVSKKAVETGDDGAAATLPSGQTINVKDDSSVLKRFGCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166AQ49</accession>
    <name>A0A166AQ49_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAB6-interacting golgin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0311805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GORAB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166AQ49"/>
    <dbReference type="Gramene" id="KZN01622">
        <property type="protein sequence ID" value="KZN01622"/>
        <property type="gene ID" value="DCAR_010376"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213432"/>
    <dbReference type="OMA" id="MINVNQS"/>
    <dbReference type="OrthoDB" id="1921288at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007033">
        <property type="entry name" value="GORAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21470:SF2">
        <property type="entry name" value="RAB6-INTERACTING GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21470">
        <property type="entry name" value="RAB6-INTERACTING PROTEIN GORAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04949">
        <property type="entry name" value="Transcrip_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG92535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="158" mass="18537" checksum="2933D8B3C0746349" modified="2016-07-06" version="1">MEQQNSQMVRLISYNSSPMAEDKEEDISALSAFRTMEEEIKKKKMEVSMKVQAQLGRVEEETKRLAEIREELEGLADPMRKEVAFVRKRIDLVNRELRPLGQSCLKKEKEYKEALEAFTEKNKEKAQLVTKLMEMVSESERVRMKKLEELSKNIDSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166C1Q9</accession>
    <name>A0A166C1Q9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0313474</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166C1Q9"/>
    <dbReference type="Gramene" id="KZN03170">
        <property type="protein sequence ID" value="KZN03170"/>
        <property type="gene ID" value="DCAR_011926"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108211804"/>
    <dbReference type="OMA" id="SYPTICV"/>
    <dbReference type="OrthoDB" id="1430376at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000006">
        <property type="entry name" value="Pectinesterase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF110">
        <property type="entry name" value="PECTINESTERASE INHIBITOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG94181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="197" mass="21736" checksum="75A304DF219F6526" modified="2016-07-06" version="1">MATLTPITLSLLLLLSLSTTLLLRTAAKSPHSDVPSSDLIHASCIHANYPQICLRTLSTYAPPATTQNDVAQAAVKISLTRSQKASEFLSNLKNKSTKREKDAVSDCVSQMSDSVDELTKTLSELKHLRRGSEFKWQMSNVETWVSAALTNEDTCLDGFKEIDGKVRSDVRRKITNVARVTSNALYLINLMDSKAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CLH2</accession>
    <name>A0A166CLH2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_004891</name>
        <name evidence="6" type="ORF">DCAR_0205414</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CLH2"/>
    <dbReference type="STRING" id="79200.A0A166CLH2"/>
    <dbReference type="Gramene" id="KZN04054">
        <property type="protein sequence ID" value="KZN04054"/>
        <property type="gene ID" value="DCAR_004891"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209910"/>
    <dbReference type="OMA" id="ASIHRLY"/>
    <dbReference type="OrthoDB" id="593669at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="245"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="326"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="464"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="276"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN04054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG86213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="60252" checksum="AFD31AE41C378E2F" modified="2016-07-06" version="1">MQTFHGSQSSGTTQRLYYQPMQQVEPYWIQNKKASSDNSSQETAHSSPHDEQFFTLDSSPADYVTYDSPSVASISSNRSPFSSQYSQSYRSDQHQSPENTYGSPVSGSSMIDDGNALKHILKELESKLLGPESEADDVCSYSFNSATPEVTSLTKYDRLLQMVPSLDVKQVLVACAEAIADGDVSSVAPLINVLERMVSVTGDPMQRLGAYMMEGIKARMLSSGSIIYKKLKCNEPTSSELMSYMGVIYQIVPFYKFAYMSSNVIIKEAVEYEPVIHIIDFQIAQGSQWVSLIQALSSRPGGPPIVRVTGVDDSNSAYARGGGLQLVGERLTKVAESCGVPFEFHGAAINGSEVKRENLRIRQGEALVVNFPYVLHHMPDESVSTANHRDRLLRLIKSLSPKVVTLVEQESNTNTSPFLLRFRETLDYYTAMFEAIDASRSRDDRQRISAEEHCVARDIVNMIACEGEERVERHELFGKWSSRLRMAGYTQCQLSHTVGGVVQDMLKEYSGYFRLEHRDGALYLRWKNRDLVTCSAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DAP6</accession>
    <name>A0A166DAP6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_005851</name>
        <name evidence="10" type="ORF">DCAR_0206582</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DAP6"/>
    <dbReference type="Gramene" id="KZN05014">
        <property type="protein sequence ID" value="KZN05014"/>
        <property type="gene ID" value="DCAR_005851"/>
    </dbReference>
    <dbReference type="OMA" id="MANQCCS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019199">
        <property type="term" value="F:transmembrane receptor protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044812">
        <property type="entry name" value="CERK1/LYK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204">
        <property type="entry name" value="CHITIN ELICITOR RECEPTOR KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204:SF8">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LysM" evidence="8">
        <location>
            <begin position="179"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN05014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG87358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="235" mass="25884" checksum="B0332316DA3D95A2" modified="2016-07-06" version="1">MARGHCTWNTYSIFHTMPTHMASWILFALLLILPSPLCMRFVASAVTAPLSAMNPLTCSLFQSATCNSILYQSNDLQKEEIAVYYSVNATDIRNITNGSKQDYLVPVPCSCQNVNGTTAFFYDTVYTVKVGDTFVNVSSQYYSGQAWPLGGEERTFIPDAEVPIHLLCGCVQDDAEVVVTYTVQQQDTLSEIGDNLSAQVSKIQSLNTNLAQNSTYIEAGWVLFVPMEKNGTSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DI86</accession>
    <name>A0A166DI86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_006133</name>
        <name evidence="6" type="ORF">DCAR_0206906</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DI86"/>
    <dbReference type="STRING" id="79200.A0A166DI86"/>
    <dbReference type="Gramene" id="KZN05296">
        <property type="protein sequence ID" value="KZN05296"/>
        <property type="gene ID" value="DCAR_006133"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208264"/>
    <dbReference type="OMA" id="MEPKIRK"/>
    <dbReference type="OrthoDB" id="1913820at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000043">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="91"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN05296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG87675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="196" mass="21475" checksum="F4BB739B3D6211F0" modified="2016-07-06" version="1">MAPKKSPKKKAVGAVVKTTTKVIQETVQVSVIQTKPKPQQETPQTENNKNGPKDIEIQDVTTPTPTPKKATKTIPTQDTAKKTKKDSAQGATKKRKRSVEGYKRYVYKVLKQVHPDIGISSKAMTIVNNLMTDMFERLADEAARLTKYTKKMTLSSREIQGAVKLVLPGELGKHAVAEGAKAVTNYVQYASGPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FEW0</accession>
    <name>A0A166FEW0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_008537</name>
        <name evidence="10" type="ORF">DCAR_0209645</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FEW0"/>
    <dbReference type="STRING" id="79200.A0A166FEW0"/>
    <dbReference type="Gramene" id="KZN07700">
        <property type="protein sequence ID" value="KZN07700"/>
        <property type="gene ID" value="DCAR_008537"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209972"/>
    <dbReference type="OMA" id="ISKVCVW"/>
    <dbReference type="OrthoDB" id="581474at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000001">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 28, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KZN07700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG90401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="148" mass="16576" checksum="428E3FED72945D9B" modified="2016-07-06" version="1">MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYETTARSWTQKYAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FHR2</accession>
    <name>A0A166FHR2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CAAX prenyl protease</fullName>
            <ecNumber evidence="15">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_008629</name>
        <name evidence="19" type="ORF">DCAR_0209748</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 15">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="15"/>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN07792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166FHR2"/>
    <dbReference type="STRING" id="79200.A0A166FHR2"/>
    <dbReference type="Gramene" id="KZN07792">
        <property type="protein sequence ID" value="KZN07792"/>
        <property type="gene ID" value="DCAR_008629"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108209109"/>
    <dbReference type="OMA" id="HWHYSHI"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000005">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 14 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="17">
        <location>
            <begin position="27"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="16">
        <location>
            <begin position="213"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="288"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
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        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN07792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG90504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="424" mass="48472" checksum="5A19ADA3466725B4" modified="2016-07-06" version="1">MAFPYMEAVVGFMILMYIFESYLDLRQHTALKLPKLPKTLEGVISQEKFEKSRAYSIDKSNFHIFHEFVSILMESAILYFGVLPWFWKKSGSFLVTAGLNAENEILRTLSFLVGVMFWSQITDLPFSLYSTFVIEARHGFNKQTIWLFFRDMIKGIVLSIVIGPPIVAAIIVIVQKGGPYLAIYLWGFMFVLSLVMMTIYPILIAPLFNKFTPLPEGELRSKIEKLASSLQFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKDEEEIVAVIAHELGHWKLNHTMFTFVAVQILSFSQFGGYTLVRNSNDLFQSFGFDTQPVLIGLILFQHTVMPLQRVVSFALNLVSRAFEFQADAFAKSLGYSTPLRAALVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAIDEPDKKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166FP67</accession>
    <name>A0A166FP67_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_000684</name>
        <name evidence="8" type="ORF">DCAR_0100475</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166FP67"/>
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        <property type="gene ID" value="DCAR_000684"/>
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    </dbReference>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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        </location>
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            <end position="302"/>
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    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
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            <end position="300"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="322" mass="34665" checksum="D32358EE9C90C244" modified="2016-07-06" version="1">MENRVIVDSSPYLSLLAGLSYGISSMAMVFINKAVVMQYSDSMTLLTVQQLATALLIHFGRVMGYTRAKALNIATAKKLILVSLFYNANVGFALASLKGVNIPMYIAIKRLTPLSVLVAGYFYGKGRPSVQVTLSVILTAAGVVIAALGDFSIDIFGYSMAGTSVFFQTMYLVLVEISGAEDGLSSIEIMFYNSLLSLPFLMLLIITTGEFPNSLALLFAKSTSLSFLVILILSLVMGIVLNYTMFLCTIVNSALTTTIVGVLKGVGSTTLGFFLLGGVQVHGLNVAGLVINTAGGLWYSYAKYQQKKRPPKIVPDEESYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GQ56</accession>
    <name>A0A166GQ56_DAUCS</name>
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        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">DCAR_0101879</name>
    </gene>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="79200.A0A166GQ56"/>
    <dbReference type="Gramene" id="KZN09215">
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        <property type="gene ID" value="DCAR_001871"/>
    </dbReference>
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    <dbReference type="OMA" id="EWNSINQ"/>
    <dbReference type="OrthoDB" id="683555at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:plant organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006355">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="NAC domain-containing protein 35"/>
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        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF70">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 19-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="29"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN09215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG82712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="343" mass="38971" checksum="08A53AC062CF7FCD" modified="2016-07-06" version="1">MAIASSMNQDHPHHDDDNKADDEHDHDMVMPGFRFHPTEEELVEFYLRRKVEGKRFNVELITSLDLYRFDPWELPALAAIGEKEWYFYVPRDRKYRNGDRPNRVTTSGYWKATGADRMIRTDSYRPIGLKKTLVFYSGKAPKGIRSSWIMNEYRLPHHETERLQKAEISLCRVYKRPGVDDHPSLPRILPTRASTSRNANNSTPNSSKKLKNIVEQTQVHAEDKFSTDQTTKISTSTDIGTALGLSSSCIALEPNISTPNGNNIYSTVPSLVPLPNAIDDLHRLISFQQASVNIQHGQQMYHNPNNIDDVLPGGSVQSAFADRLWEWNSVTGAAAKDYDAPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166ICG7</accession>
    <name>A0A166ICG7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0103859</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166ICG7"/>
    <dbReference type="Gramene" id="KZN10986">
        <property type="protein sequence ID" value="KZN10986"/>
        <property type="gene ID" value="DCAR_003642"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204071"/>
    <dbReference type="OMA" id="ISKVTWH"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000071">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000078">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000002">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000011">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG84675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="880" mass="97689" checksum="C4130F8EC7CB0F1A" modified="2016-07-06" version="1">MAQMVKKDDDRDEEADYSPFVGIEKGAVLQEARVFNDPQLDPRRCSQVITKLLYLLNQGESFTKFEATDVFFAVTKLFQSKDIGLRRMVYLMIKELSPCADEVIIVTSCLMKDMNSKSDMYRANAIRVLCRIIDGTLLTQIERYLKQAIVDKNPVVASAALVSGIHLLQTNPDIVKRWSNEVQEAVQSRAALVQFHALALLHKIRQNDRLAVSKLVTSLTRGTVRSPLAQCLLIRYTSQVIRESSTSNQTGDRPFYDYLEGCLRHKAEMVIFEAARAITELNGVSSRELTPAITVLQLFLSSSKPVLRFAAVRTLNKVAMTRPLAVTNCNIDMESLIADQNRSIATLAITTLLKTGNESSVDRLMKQITNFMSDIADEFKIVVVEAIRSLCLKFPLKYRSLMNFLSNILREEGGFEYKKAIVDSIVILIRDIPDAKESGLLHLCEFIEDCEFTYLSTQILHFLGNEGPKTSDPSKYIRYIYNRVILENATVRASAVCTLAKFGAMVDSLKPRIFVLLRRCLYDSDDEVRDRATLYLNTLGGDGAVVETDKDVKEFLFGSLDIPLVNLETSLKNYEPSEEPFDVKSVPKEVKSKPIAEPTGLRAPPAAAPAPTVDTYERLLSSIPEFSSFGKPFKSSAPVELTEAETEYAVNVVKHIFDGHVVFQYNCTNTIPEQLLENVSVIVDASEAEEFSEVAVTPIKSLPYGSPGQTFVAFEKPGGVTAVGKFLNVLRFIVKEVDPSSGEAEDVGVEDEYQLEDLEVVAADYILKVGVSNFRNAWESMDPDTERVDEYGLGSRESLAEAVGVVINLLGLQPCEGTEVVPNNARSHTCLLSGVYIGNMKVLVRLSFGIDSRKEVAMKLAVRSDDISVSDAIHDIVASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175Y9H7</accession>
    <name>A0A175Y9H7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0934794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175Y9H7"/>
    <dbReference type="Gramene" id="KZM80185">
        <property type="protein sequence ID" value="KZM80185"/>
        <property type="gene ID" value="DCAR_000083"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF34">
        <property type="entry name" value="OS08G0555500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH15257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="167" mass="18849" checksum="01066ED512C1295D" modified="2016-09-07" version="1">MGFGLLKYLPTVKMEQKLVKKLVENFDVGSCSLFGLTITAQHGGEILGFPSKGKLVPKAISDVERDKFKAKYEKLNFKKLTELLKKADLDFTETFMLYAFGHFYCSCTKDVPSQKLYNALSVVPSAREYNWGKFVCESLLEAITIKEEKVKQIAIHGIWILGAAHTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YB97</accession>
    <name>A0A175YB97_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0414448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YB97"/>
    <dbReference type="Gramene" id="KZM80763">
        <property type="protein sequence ID" value="KZM80763"/>
        <property type="gene ID" value="DCAR_031661"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG95145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="134" mass="15591" checksum="F72A73BD02B273D3" modified="2016-09-07" version="1">MLSKRIILPVPLKQVKMDIEIWFSIMCLIKVKVNEYEESFTKYQIHQMLLFRYVGVDEDEDVQLFYYFVKSRANPENDPLILWITGSPGCSSFTALAYEFGVTSLEVEIHAKVKKKDVQEEVDSKMDVRGSEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YD21</accession>
    <name>A0A175YD21_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_029127</name>
        <name evidence="3" type="ORF">DCAR_0933479</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM81514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YD21"/>
    <dbReference type="Gramene" id="KZM81514">
        <property type="protein sequence ID" value="KZM81514"/>
        <property type="gene ID" value="DCAR_029127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM81514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH13965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="122" mass="13976" checksum="28363EFF8FEE9CAF" modified="2016-09-07" version="1">MADWIRPVTLSRLWNRCYNLFSSSDFFISTINTIKDVLSWSGLFIISLLIGIWIKLAITGLASFLPGDVPSWKTSWTVGIAIFTFSCYCLEECLKMKRAQLARALLDPSRRNNIEFRNGTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YDD8</accession>
    <name>A0A175YDD8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0933433</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YDD8"/>
    <dbReference type="Gramene" id="KZM81473">
        <property type="protein sequence ID" value="KZM81473"/>
        <property type="gene ID" value="DCAR_029086"/>
    </dbReference>
    <dbReference type="OMA" id="HVLLICY"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000101">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000237">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF986">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH13920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="488" mass="53771" checksum="2D7E892F01405029" modified="2016-09-07" version="1">MPHAMNSQDLVHVLLVSFPSQGHVTPLLRLGNLLASTGLLVTFSTTENAGDFMRKANKNIDEATPVGDGMIRFEFFDDGLSGDNEERRRLDLEYKIAQLELHGKEAVTRFVEKHNKEGRPVACLINNPFIPWVTDVAHALNIPNAVLWVQSCASFSAYYHYNNKLAIFPDESDPEIDVQLPSMPLLKHDEIPSFLHPNAPFSFFGKGILGQFKNMSKTFCVLVETFQELESEVIDYMSKLCPIKPIGPLFKNPKSSNSSIQGDFVEADDCMDFLNSKEPATVVYISFGSIVSLNQEQTNEIAYGLLNSGVSFLWVLKPPTMGSASAVVLPENFLGAAGDKGKVVQWCAQKEVLEHPSVAYFVTHCGWNSTLEAISSGVPVVTFPAWGDQVTNAKYLVDVFEMGVRLSRGEQTEKNGVSRDKIEKCLREAIGPKAAEMKKNALKWKEAAKEAVADGGSSDRNLKEFVEKIRNVSKLGSASVNLVNGTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YFU1</accession>
    <name>A0A175YFU1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">rRNA N-glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0934706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome-inactivating protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.22" evidence="1"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YFU1"/>
    <dbReference type="Gramene" id="KZM82363">
        <property type="protein sequence ID" value="KZM82363"/>
        <property type="gene ID" value="DCAR_029932"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="1" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH15169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="155" mass="17767" checksum="E698B8871AC880DF" modified="2016-09-07" version="1">MIYRGHDLYTVGHLDRYRHAFLLVQERKSQLRWTIKCLRHYGIRHTMMHAIGDSYPALEAVAQIRRAELSIGQQGFLTALNHLLVENPLANIRNTGFSYLFLCVFVCEGRRIVLVRDSIVHNFTGLGLPVPEYLLQYVYGWAGASFILEAFATAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YGF8</accession>
    <name>A0A175YGF8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">S-acyltransferase</fullName>
            <ecNumber evidence="15">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_029858</name>
        <name evidence="18" type="ORF">DCAR_0934503</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="15"/>
    <dbReference type="EMBL" id="LNRQ01000009">
        <property type="protein sequence ID" value="KZM82258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YGF8"/>
    <dbReference type="STRING" id="79200.A0A175YGF8"/>
    <dbReference type="Gramene" id="KZM82258">
        <property type="protein sequence ID" value="KZM82258"/>
        <property type="gene ID" value="DCAR_029858"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108202615"/>
    <dbReference type="OMA" id="FWVGFRY"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000300">
        <property type="entry name" value="S-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF101">
        <property type="entry name" value="PROTEIN S-ACYLTRANSFERASE 23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="160"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="366"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KZM82258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH14973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="538" mass="59903" checksum="B2BC7C4164F8F292" modified="2016-09-07" version="1">MASSEIEIVSDASDQMNLEQPQNQSIVISDIFSATAYGDFSKLKKFVEEDGVSVMIPDGNGYYALQWASLNNFPDIAQYIIQHGADANATDHMKQTALHWAAVRGSIAVAGVLLQNGAQVEAADMNGYRAVHIAAQYGQTAFLNFIVAKFHAEFDVPDNDGRSPLHWAAYKGFADTVRLLLFRDASQERQDKEGCTPLHWAAYQGNVEVCTVLVYAGTKEELIVKDSTGLTPVQIASDKGHRSLTSFLLRAQHAQGISWKDRVFSGKLSFVGYAPILFFIIVITTLMFINTILLASNLPKVTAVVGLWGWTAVSISFCTVLMLIRCSSKDPGFIKFARGTGHFTDTEDPLLNVDLNNPAISLRDWSQLCPTCKIIRPTRSKHCPVCKHCVEQFDHHCPWISNCVGKRNKWDFFIFVFMGTSIAFLGAVVGFHRLWTSAPVLQDEEIWIHHLVVQHPGAVVFMFVDAIILISCGTLMTAQLYQISRNITTNELSNRARYGYLHGPDGRFHNPYDRGLLKNCSDFFIRGYTDDLIAHPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKN7</accession>
    <name>A0A175YKN7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0831125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
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    </comment>
    <comment type="catalytic activity">
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    <comment type="subunit">
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    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Plastid</location>
            <location evidence="8">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W4Q2"/>
    <dbReference type="KEGG" id="dcr:108205337"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000001">
        <property type="entry name" value="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.380:FF:000001">
        <property type="entry name" value="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004014">
        <property type="entry name" value="PRK05477.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="9">
        <location>
            <begin position="395"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG83210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="546" mass="60560" checksum="795D2167CF3B421E" modified="2024-05-29" version="1">MALTMFRGIKCCPFSMYPSALVARKHVFLYCTMNNSPSQTATQDKAQANAKVSTQSKKMSKVAKDYEAIIGIETHVQLSTLTKAFCTCPYNYGSQPNSSVCPICMGLPGALPVLNSKVIEYAVRLGLALNCELSFNSKFDRKQYFYPDLPKGYQISQFDVPIATGGFIDLDLPVEFGGGHRKFGITRVHMEEDAGKLLHTGNGSFSQVDLNRAGVPLLEIVSEPDMRTGIEAAEYAAELQRMVRYLGISNGNMQEGSLRCDVNVSVRPWGQIEFGTKVEIKNLNSFSAMNRAIDFEIARQELLHSQGQADQIVQETRLWEEGAQKTVTMRKKEGLADYRYFPEPDLPAVNLTIKYVDDIRDSLPELPEMKRRRYETMGLSMQDILFLVNDMDVADFFDATIAKAADVKLAANWIMGDIAAYLKNEKLSINEVKLTPEELGELIASIKSGTISGKIGKEILFELLAKGGTVQGLIKEKDLVQIADPVEIEKMVDKVIADNPKQLEQYRGGKTKLQGFFAGQIMKASKGKANPGILNKILLEKLNAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5I0</accession>
    <name>A0AAF0W5I0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W5I0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG82719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="14459" checksum="29BB70E0E1A4D35A" modified="2024-05-29" version="1">MNANEMSYIPIFRPEMGSYSETEKRALFLRSYHFSRKQSFSERIKKSFFRIKRVIRVRLRSAKRLRKIVWFRLKNGMFCSTRTRTRFLRMQTRDAQKIWTRRRCKAYVSSWPSSCLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W9J6</accession>
    <name>A0AAF0W9J6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1-aminocyclopropane-1-carboxylate synthase</fullName>
            <ecNumber evidence="8">4.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0104998</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.14" evidence="8"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W9J6"/>
    <dbReference type="KEGG" id="dcr:108214624"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016847">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF6">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="45"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049554"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="494" mass="55955" checksum="9ED245A27863BC6C" modified="2024-05-29" version="1">MAFVNNLLSKMAISDGHGENSPYFDGWKAYDSDPFHSVENPQGVIQMGLAENQLCFDLIQEWILKNPKASICTSEGVDEFKDTAIFQDYHGLPEFRDAIAKFMAKVRGNTVTFDPDRVVMSGGATGAHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESCNDFKITREALEEAYQKAVESNINVKGLLITNPSNPLGTILDRDTLQSLVDFINEKNIHLICDEIYAATVFTKPMYVSIAEILEEDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDSVVNTARKMSSFGLVSTQTQRLIANMLSDDTFIDQYVSESRKRLAARHGVFTRGLMQVRVGNLQGNAGLFFWMDLRRLLKDSTVEAEMELWRVIINEVKLNVSPGSSFHCSEPGWFRVCYANMDNETMRIALRRIKKFVLQADERQVTAKKQTWHKDLKLSLSFRRCDDTILMTPRMVTPHMMSPHVVVSPHSPLASPLVRARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAG0</accession>
    <name>A0AAF0WAG0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0104668</name>
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    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85479.1"/>
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        <property type="component" value="Chromosome 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="121" mass="14542" checksum="6C070C8C7B6CB8EC" modified="2024-05-29" version="1">MKPIPELPQELFRDEILSRLPIESVSRFKSVCKSWLSLFSDPHFVKQHNDRQNPKENDLLVIRKYGHIAIISRYQEQFLLRKYDDYKLIGSAKGLVSLRWQNVVSLWNPRFVNLRNFTFHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WF66</accession>
    <name>A0AAF0WF66_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0206391</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WF66"/>
    <dbReference type="KEGG" id="dcr:108209403"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Casein kinase 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="301"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG87168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="456" mass="51658" checksum="725626B133D978BC" modified="2024-05-29" version="1">MDHVVGGKFKLGRKIGSGSFGELYLGVNVQSGEEVAIKLEPVRTKHPQLHYESKIYMLLQGGTGIPNLKWFGVENEYSVMVIDLLGPSLEDLFNYCNRKFSLKTVLMLADQLINRVEYMHSRGFLHRDIKPDNFLMGLARKANQVYAIDFGLAKKYRDLQTHKHIPYRENKNLTGTARYASVNTHLGVEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGNKKQKYDRISEKKMLTPVEGLCKSYPSEFISYFHYCRSLRFEDKPDYSYLKRLFRDLFIREGYQFDYIFDWTILKYPQIGASSRGRNPSGNPGPSGERTGKTSVGQDIREKFSGAVEAFSRRNTSGHGRQGEQSRHRSSEKVPSSKDVQADSDKGRSSQNGNSSKRVVIASSTRPSSSGEFTEGRAGRLVSTSGRLSAAQRILSGSEPKQPSYSRNTVTKGTRDDPLRSFDFLSIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHE2</accession>
    <name>A0AAF0WHE2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0208957</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHE2"/>
    <dbReference type="KEGG" id="dcr:108206381"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF3">
        <property type="entry name" value="OS01G0748500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042185269" evidence="8">
        <location>
            <begin position="26"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG89719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="363" mass="40217" checksum="9B4F3D2B42B58597" modified="2024-05-29" version="1">MGVELSKWLLCVIFLLALCRNGAHAQPQVPCYFIFGDSLVDNGNNNQITSLAKANYRPYGIDYPQGPTGRFSNGLTTVDVIAELLGFSNPIPPYSQARGRAILGGVNYASAAAGIRDETGQQLGQRISMSGQVNNYKNTISQVVNILGDENTAANYLSKCIYSVGLGSNDYLNNYFMPNIYSTSRRYTPDQYADVLIQQYRAQLVDLYNYGARKFVLNGVGQIGCSPNALSQSPDGRTCVQRINSANQLFNNRLRSLVDDLNRNQRDSKFIYINAYGIFQDLVTRPANFGFRVTTAGCCGVGRNNGQITCLPFQNPCRNRNEYVFWDAFHPTEAANIVLGRRSYAAESGSDAYPFDIRRLAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WIC9</accession>
    <name>A0AAF0WIC9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0208302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WIC9"/>
    <dbReference type="KEGG" id="dcr:108205594"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055801">
        <property type="entry name" value="DUF7377"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF84">
        <property type="entry name" value="PROTEIN KINASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24093">
        <property type="entry name" value="DUF7377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="329"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG89066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="591" mass="65150" checksum="8177E7DAE28E428F" modified="2024-05-29" version="1">MSAALECWSSRASTDEDMVEQVLMRTQHRSESLNDAVLSSAVSPGGAAGFKETSAMQKRIQRLSRNVSEAIASLKNSLNLDSPGPSGRVENCRKNVWAGVVRNLTQLYPGSQLPEKLVSNIRKHYDSLPLSYAQAGFEMKDVFLHIKLIEQASVQDHPAILIQEVSDDEVQGSVFKLVFACTSSLSWPTMSGALDNASICCKKIQIFEKKGFTLGIVLVLVQSGQEKLFKNRIDSALKLGLKKPKNSGMKLPFGLCGCQEESTRGRELGVGEVDEDSGESRNGSENSNSRVQLQLPLSNSAFVVSVDEWQTVESGGDEIGRWLLNPDNLEFMDQIGSSTYKGLYKGKKVGIEKLKGCDKGNSYEFEIRKDLLELMTCGHKNILQFCGVCIDDNHGLCVVTKLMEGGSVHDLMLRNKKLQNKEIVRIAADVAEGIKFMNDHGVAYRDLNTHRILLDRHGNACLGDMGVVAACKSVTEAMEYETDGYRWLAPEIIAGDPESVTETWMSNVYSYGMIVWEMVTGEVAYSAYSPVQAAVEIAACGLRPDIPKDCPQLLRALMSKCWNNCPSKRPHFSDILSILTRPVNNGNNTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJ56</accession>
    <name>A0AAF0WJ56_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0209340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJ56"/>
    <dbReference type="KEGG" id="dcr:108208718"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1551">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="481" mass="53977" checksum="75828C1AED0F198B" modified="2024-05-29" version="1">MDTLETTAKKQGHVVCIPFPFQSHIKAMLKMAKLLYSKGISITFVNTDYNHKRFLKSGGAQALDGLPGFKFESIPDGLHSSDSDVTQDITALCHSILEEMPRPFQNLLTKLNTGTHQVTSILSDGFMPFTADAAHSHGVPAILFWPFAACGFMGFYQFKNARERGLVPFKDESYLTNGYLDTIVDWIPGMGNIRLGDLPTQIRIMDSDDFLFNFAVECSKRADNATAHVIHTFDDIELKLLNAISSMFKGVHTIGPQQLLLNQIPTDQEESLRSIGYSLWEEEKTCLRWLDSKEADSVVYVNFGSVTVMSPEQLMEFGWGLANSNCAFLWIIRPDLIMGESNITLGAEFMDAIRNRGLIASWCPQEDVLNHVSVGGFLTHGGWNSIIESICAGVPMLCWPFFGEQTTNCKYMCNEWECGMEIHHDVNRDDVEKLVRLLMDGVEGKKMRNKTMQWKKMAEKACQPDGSSSLNLDKLVLLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKR3</accession>
    <name>A0AAF0WKR3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0310952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKR3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF252">
        <property type="entry name" value="TERPENE SYNTHASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="213"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG91702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="513" mass="58749" checksum="DD19793E10D5E9E4" modified="2024-05-29" version="1">MATKLEIEIRMTIEDEMASNPLNFFELIDNIERLGLGYRFEKDISTALTKYVSLEGAPEYHNSLHSTALRFRLLREHGYKVSQDVFQSFKDENGAFMPSLLADVKGLLSLYEASFLAFQGETFLDEARSFARKGLEYVMQKTESKLLAKLIGHTLALPWYRRTLRFAARPYIDVYSRMEDSNELLLQLAKLDFNIVQSALQQDLVQVMRWWQNIGLSGKLSFSTKDSLLQCFFWTIGISFEPHLSDSRISLTKLATFLTVIDDLYDDHGSVDELELFTDSVRRWNVDAVDDLPDSLRLFFLALYNTVNEMAYDALKEHGENPLPLFKKVWGDLCQALLQETKWNHEKSIPELDDYVENGWRSSSGVVILAHAFPLRSQNITKEALDILAKDHHLLKWSSMVFRLCNDLASFTRESKSGETANSVTSYMYHNGVSEDVAREHIKNLIDDAWKQMNEARVSLEPQFSTSFTEAAINLARLSHSAYGSGDDHRIPDKKAKKQIFSLLMEPFPVTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WLB6</accession>
    <name>A0AAF0WLB6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0310934</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WLB6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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            <end position="376"/>
        </location>
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        <location>
            <position position="217"/>
        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG91684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="39847" checksum="8730E502879F795D" modified="2024-05-29" version="1">MNSYIYSIKNLMIFFMIIFCNEVCGSRTFHVKNYDKNDIDTQVFSVPDGTYVIDPIQFSGPCQGQVTFQLDGTLQAPTGKIDANDWIKFHNVDGLIIQGSGTLNGEGDLAWVGHCPGCPPLTTSLTLSFVNNGHIKDITSLNSKGFHIKIINGGDTIIEHVTISAPGDSPNTDGIHTSGASNINILNSEIGTGDDCISIGGGSQNINITGVTCGPGHGISIGSIGKNTDDSSVTGVYVNGCTMSNTQNGVRIKTYTSDCMATVSDVTFQDITVDQSKNPIIIDQNYCGGHQECIGNSHVQVSDVKFIGVQGTSISPIAVKLQCSPEKPCEGIELDTISISLNDGGETTSSCSNVNVTYNGPQNPPTCSNSLHLNHM</sequence>
</entry>
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        <recommendedName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG91053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="325" mass="36107" checksum="3B675295192E209E" modified="2024-05-29" version="1">MGSQGGYSGGAGRGTREPKLDSLVRQNSLYNLTLDEVQNHMGNLGKPLSSMNLDEFLKNVSSVESNQGLGRGEYGVQNRQLASVSSLSRKPSLSLSRDLSKKTVDEVWKDIQQGQKRPGIGMNSRERNSTLGEMTLEEFLVKAGVISEGEKALGSVVGVNPVSLPQQSVSLPAAWMNYQIPPTHQPTQQQIMPILFMPSNSVQPQLSLSSDPILDSYGETQMTSPSPLLGTLLDAQSQGRKRIASEDVVEKTVERRQKRMIKNRESAARSRARKQAYTNELENKVQRLEEENEMLKTQKEAEKLLPYVPFPEPKYQLRRTSSASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WME0</accession>
    <name>A0AAF0WME0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">NusG-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">DCAR_0311827</name>
    </gene>
    <organism evidence="5 6">
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        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>asterids</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
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    </dbReference>
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        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF4">
        <property type="entry name" value="KOW MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="4">
        <location>
            <begin position="101"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG92555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <source>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Senalik D."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
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</entry>
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                <person name="Zeng P."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG96394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Lysine histidine transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
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        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="398" mass="44409" checksum="5CC29B3B58BCEACE" modified="2024-05-29" version="1">MVGAGVLSLPYAMAALGWGPGIVVLILSWVITLYTLWQMVEMHEMVPGKRFDRYHELGQHAFGEKLGLYIVVPQQLIVEVGVNIVYMVTGGKSLQKFHQTVCPDCKDIKLTFFIMIFASVHFVLSHLPNFNSISGISLAAAVMSLTYSTIAWSASLHKGVQQDVEYGYRSKSSIGTVFNFFTALGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWKGVMVAYFVVALCYFPVALIGYKMFGNTVDDNILITLKDPAWLIAAANMFVVIHVIGSYQIYAMPVFDMLETLLVKKLHFKPSRPLRFITRNTYVAFTMFIAITFPFFGGLLGFFGGFAFAPTTYFLPCVMWLAIVKPKKFSFSWFANWTCIVLGLLLMTLSPIGGLRNIIINAKTYKFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X1Y0</accession>
    <name>A0AAF0X1Y0_DAUCS</name>
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        <recommendedName>
            <fullName evidence="8">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0519404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108220517"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042180857" description="FAD-binding PCMH-type domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="75"/>
            <end position="251"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH00048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="540" mass="60244" checksum="3B4C58A6D8523425" modified="2024-05-29" version="1">MKTALHFLFFLSVVTICSSLELEWLESPNPTGFLRCLRSSTTDHINQLTFTSANSSYTDIYSFSARNPRFLNASGRLSPAVIVTPSAESQVQAVVICARKNDMQIRTRSGGHDFEGLSYSSTYTRPFVLLDMINLRSVIADPVAKTAIVQAGATLGEVYYWINRASGTLAFPAGVWSTVGATGLICGGGYGPLRRKYGFAADNVLDARIVDVRGNILDRKGMGEDLFWAIRGGSCSSFGVILSWKLNLVDVPSKLTIFTVTREERQTEIMYPFQTIAPVLPNELDIRCRISTVRNNASTRADGLVVQLGFTGSYLGSAEEALAIFDSRLPQLRIQRSELQEVTWIEALMQSSFFPFFSANYTPENFLDRTFLADIPTKAKSDFVRSPISVPGLNGLWDKLLEVGEGETTVIFTPYGGVLDSYPESTIPFPNRAGTLFMIYARVLWVGNTTQKLEWIRSLHDYLTPYVSSNPRRAYYNYDDLDLGANPATGMISNIAARRWGRSYFNNNFNRLIAIKTLVDPVNFFRHEQTVPTFSLIQDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X8F8</accession>
    <name>A0AAF0X8F8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0622787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X8F8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF42">
        <property type="entry name" value="GERANIOL 8-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042026516" description="Cytochrome P450" evidence="7">
        <location>
            <begin position="24"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH03390.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="58035" checksum="2D7A0318FE79AD12" modified="2024-05-29" version="1">MENYNALVACSLIFLVFVQYVLGLSARAELPPGPNGLPFLGNLLQIGPKPHRSLAKLAEQYGPLFAIRLGRVTNVVVSSAEMAEEVFRKYDAEFSGRAVPDAVAGGLQNHDLALPWISIGDQWRTIRRALSVYLTNPKKLDMLQGLRLKVVKQMVEHVKEISERGQVVNIGRLAFATALNQMSNTCFSTDVAHFNSDEDGSEFQNAVKTIMKVDGKMNFADYFPWLKIFDPQGIRRDAKAAYSWLDQLCEKFIIERLKHRESNFSPHGDLLDSFLDFRQENLVYFDLKHIKVLLMDLFIAGTDTNSSTIEWAMTELIQNPSIMQKLREELTHRIAEKGSLEEAEILEIPYLQSVLKETMRLHLVSPFLLPHKTVTNVKFKGYTIPKNTPIIINAWAIARDSNSWENPTDFTPERFLSSEIDYKGRYFSFLPFGSGRRICPGIRLAERVMSLMLLSLVAQFDWNLPNNMSLKDLDMDDTFGVTSQKATPLLLIPTTRNDQQPLKKQDKTDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XBD0</accession>
    <name>A0AAF0XBD0_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0624570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XBD0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH05156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="180" mass="20274" checksum="1C5C6272E7ADF3FD" modified="2024-05-29" version="1">MFLKHGAVTFLKKSQNWYTISQKVPFSCFHGNPCSFGHFWLNIDTGGADEESDSSDSESNDVLDLSKILMDILFGVFCECAELNPDPIEKEEEHNWVFSADQVVTDGAEVDDSEWNGVLAPTSSIGYSNGDNDLARTALQVIFLSYVPVRGLRFEQSLWSRNLDYMVQNILLHFFMNAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEZ6</accession>
    <name>A0AAF0XEZ6_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0624993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
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        <property type="protein sequence ID" value="WOH05574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAUT"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF30">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH05574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="505" mass="56938" checksum="79EDD511BD849BE9" modified="2024-05-29" version="1">MKGKASVVGRRRSRISIVKLMLAILLPFLFLRLALFLIESASLCSSSFGCMGPKLSGGSESSRLIEQLTKALLEANNGDEVVEKTFGVSPESFGDLIKDVMSSKQDIKAFAFKTKAMIALMERKVQSARRHELIYWHLASHGVPKSLHCLHLKLAEEYAVNAIARSSLPSPEYVSRLTDPSFHHVLLLTDNVLAASVAITSAIKNSISPEKMVFHVVTDKKTYTPMHAWFAKFPVDSAVLEVKGLHQYDWSHEVNYGVKEMLEIHRLILSHTYENLKEGFNTVGYNERQLEFTSPRCVSLLNHLRIYAPELFPNLNKIIFLDDDVVVQHDLSALWQVNLNGKVVGAVFESGCGPDCCPGRKYKNYFNFSNSYISSNLNEDQCGWLYGMNVFNLEAWRNTNITATYHQWLKFNLDSGLDLWNPGALPPALLAFSGFVHPIHPSWHMAGLGYRFPQVGLEALKAATVLHFSGPAKPWLEIGDSKVRSLWTRHLNYSNEILRECRIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFV2</accession>
    <name>A0AAF0XFV2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0726753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BetVI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFV2"/>
    <dbReference type="KEGG" id="dcr:108193621"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00451">
        <property type="entry name" value="PATHOGENESIS_BETVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="3">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH07323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="155" mass="16639" checksum="C7A262325DFA2A35" modified="2024-05-29" version="1">MGVQKSEVEATSSVSAEKLFKALCLDIDTLLPQVVPGAIKSAEILEGDGGVGTVKLIHLGDASPFKTMKQKVDVIDKDSFTYSYTIIDGDILLDFVESINNHFTYVPTADGGCTVKSTITFTTKGDAVVPEENIKFANDQNLAIFKAIEAHLIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJH3</accession>
    <name>A0AAF0XJH3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0727599</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJH3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF19">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042197807" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="17"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="499"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="553"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOH08162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="864" mass="96918" checksum="8710E8F4A48D9AA4" modified="2024-05-29" version="1">MGLIFCLLALVPYVMCQVTEFISIDCGGSSNYTDLNTGLPWISDSGIISSGQSAELEDPEASLPQYRKRRDFLYLVRATFLYGNSKSTFPKFQVYLDATKWSTVSITNASEVHVEEMIIRAQSNSVDVCLCQATTGSPFISTLELRPLNQSMYATEFEDEFYLKVAARVNFGATSNEAIRYPDDPYDRIWSSDLERRQNFLVGVAPGTERVNTTKYINTNAKEHPPLKVMQLQLLNLEDFPASARGYAYFAEIEEFETSESRKFTIAQPEIPDYNTAVINIAENAHRSYTLYEPSFKNVTLDFVLSFSFVKTPDSTHGPLLNAIEISKYVPIAKKTDVRDVNTLNSLRGKSMDSYMKDEARGDPCVPTPWDWVNCSTSIPPRITKIALSGRNVEGEIPPDLNNMDGLIELWLNGSSFEGPIPDMTNLINLQILHLENNKLTGPIPSCLGSLPSLKELYVDNNSLTGDIPSTLLKGKLILSYEGNPGLRDSTKHKRYRRMIVGVSIGLLAILLLLFLGTLYYCIIFRKRERGRNRIDEEVSYHITFADIEEFTKSFSKQIGKGSFGPVYFGRMKDGKDLAVEVMADSSNHGNRQFVTEVARLSRIHHRNLVSLIGYCKESHQSMLVYEYMHNGTLRDHIHDSSNQKSLDWLSRLQIAEDAAKGLEYLHSGCNPSIIHGDVKTSNILLDINMRAKLSHSGLSRQTEDDQTHVSSLAQGTVGYLDPGYYANKKLTKRSDVYSFGVVLLELVSGRKPVSTEDSGTDWNIVHWARSMICKRDVKSIVDPSVAEKAKIESVWRIAEVAIQCVEQRSSSRPRMQDIILAIQDAIKIEKGSDETVSGNAKSQSSRRTLLTSFLDIESPELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJP4</accession>
    <name>A0AAF0XJP4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0727705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJP4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053031">
        <property type="entry name" value="Cuticle_assoc_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396">
        <property type="entry name" value="OS03G0264950 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396:SF27">
        <property type="entry name" value="OS08G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="6">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH08267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="10877" checksum="4DAF9AF990C2F86E" modified="2024-05-29" version="1">MQTTEPTGGTSSDPPVDSAASIHKKRKAMKPRSEVWKHYDKIQNELGELRARCKYCKRDYIGDTSNGTSGLATHVRHCKVYNLASNLLGPKYRRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XPV9</accession>
    <name>A0AAF0XPV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0831574</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="13"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XPV9"/>
    <dbReference type="KEGG" id="dcr:108199828"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042193261" description="Receptor-like serine/threonine-protein kinase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="17">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="18">
        <location>
            <begin position="344"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="506"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOH12075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="817" mass="92683" checksum="B876A73CB8943162" modified="2024-05-29" version="1">MELTSTILVCFILSSTLVNSMAVDIIRMHQTFRDGDTITSAGGEFELGFFSPGSSTNRYLGIWYRKISRGTVVWVANRDNPLMNTSGLVQVNHKGISLQLVDNSTGIIWSSNTSVSMRNPVAQLLDTGNLVLREGHLSFNGVKDFIWQSFDHPGDNFLPGMKHGIDLVTGLNWIFSSWTSVVDPSLGNFTSGLDPNGFPQFFVLKDSMIWTRIGPWNGHKFSGLPNRNPNGIFKDVFVFNEKEIYYKFHLINTNTTSAIMRFVLTPTGDAKLLVWNDPQKIWMVYLSMQDSDCDRYGLCGVYGVCKINSTPRCECLRGFVPKFPEKWKAADWSSGCIRKTDLVCGSDEGFVKYSGLKLPDTRHSWYDSKMNLQECEMVCWKNCSCTAYAIAEFRRGGHGCILWFNDLIDIRDFTEDGQDIYVRMPSSELVISRRVKWMPEFIFIAAVLAVVLLGLIFFLVYKKKKLQKKESLKLNLETVALTKIENEDPELPSFDFQSITNATSNFSQDNKLGEGGFGPVYKGMLTDGLEIAVKRLSRNSSQGLDEFKNEVSCIAKLQHRNLVTLLGCCTEKDERILIYEYMANKSLDSFIFDNNVRTTLDWPKLYNIINGIARGLLYLHQDSKLRIIHRDLKASNILLDHDMNPKISDFGIARTFVGNESEANTSRVIGTYGYMSPEYAIDGKFSVKSDVYSFGVLLIEIISGVQNRSFSHPDHSFNLLGHAWMSYKHDKLLEVIDRTILEHSNHFEVFRVVQIGLLCVQQDPKDRPVMSQVVLMISSDMKLPRPKQPGFFTERYLSHLNPSSSNQLTITTLLPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSK2</accession>
    <name>A0AAF0XSK2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0833028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSK2"/>
    <dbReference type="KEGG" id="dcr:108197871"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022821">
        <property type="term" value="F:solute:potassium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090333">
        <property type="term" value="P:regulation of stomatal closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF114">
        <property type="entry name" value="PROTEIN ZINC INDUCED FACILITATOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="38"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH13518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="484" mass="53154" checksum="E950BF133E882D62" modified="2024-05-29" version="1">MASEDIKETLLEKDYNENCPGCKVDRRKAVQQGVPYKEILVIWVVVLASALPISSLFPFLYFMVRDFNIAKEEEDISYYAGYVGSTLMLGRALTSVFWGMVADRYGRKVVIIFGTFSVVVLNTLFGLSINLWMAIATRFLLGSLNGLLGPIKAYAIELVRCEYQALALSTVSTAWGAGLIIGPALGGFFAQPADKFPVLFSQESVFGRFPYFLPCLVISLLALVVAIACLWLPETLHMPEFHKLQRHDSLEKLESAYGSEEKYMSDNIKTDSSESLYKNWPLMSSILVYCVFSLHDMAYTEIFSLWAVSPRKLGGLNFSTDGVGKTLAFSGLGLLVFQLFVFPQLERRVGFIILARVSGLATIPLLTSYSYIALLSGVTLTVLLTCASVLKNILSVSIITGIFILQNRAVDQHQRGAANGIAMTAMSLFKAIGPAGGGAILSWSQGRQDAAFLPGTQIVFFILNVVEAIGIALTFEPFLVLRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XV97</accession>
    <name>A0AAF0XV97_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0934453</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XV97"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000020">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF424">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="56"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="507"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH14923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="849" mass="97948" checksum="2C0B436538F88E67" modified="2024-05-29" version="1">MSLIDSCKHKLDAYTRVDDGVSSSSAVTMRSDVINSVSGARSAACSAESSKSHSSLQFLVRLFSGMNTLVIRADVNDTIGYVLDLIQSKARIPSNVQMLIYKSKQIYPEQTLGECCVENDAVLQLVGVMRSTRLVKAQQLVDEIVSLIWRLCKRNSGNEAWDREARRVIRRNLSDYFGDFLPKDNDNYFGHYLQVFCCSHVPASLAMLYMSPQNMIFGNELIQELITLISLLPRHLSLQCIPIYLEICKALRGMPYYRNDAMFNTCRRCLSDMVKCTEMKTAPNGAGYAYLVNFIRDMQTFVAEAADEIVHSMDAGIEPDTRMSPSHPLLTEVVYLKNFLMPIETFITEELFFEFRYRKVSQDGPYQEFSIMFETFFRLLNKMDYCLEELEKLLPTKSENLGCYQYVAIIMELNNMSKHFPDLAKQFWTTLRNRKLSFSYLIIKYASREDDYEWISVHKEVTNFECRRHLAMMLLARVSGEDNNLIQEIMLIDRSRLLEDSFEYIAHSEAETLRASLDLQFKDEEATGPGVLREWFVLVCQAIFDPRNALFVACPNDRRRFFPNSVSRVDPLHLEYFKFAGRVIALALIRKVQVGIVFDRVFFQQLAGKRVSVDDIRDTDPVFYSSCKRILEMDPDEVDQDALALTFIVEADNFGSKNIVELLPDGKNISVNSKNRVNYVARLVQHHFVDSVKDQVAQFTQGFDDIMNSDRLRESFFQCLELEDFDWMLYGSERPLCVEDWKSHTDYNGYEETDPQISWFWETVASMSAGQRKALLFFWTSLKNLPVEGFGGLTSRLYIYKVNETCNRLPTSQTCFYRLCFPPYQSLEVMQDRLSLVTQEHVACSFGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVC1</accession>
    <name>A0AAF0XVC1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0933310</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVC1"/>
    <dbReference type="KEGG" id="dcr:108202500"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000003">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041951398" description="glucan endo-1,3-beta-D-glucosidase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="12">
        <location>
            <begin position="370"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH13799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="458" mass="48629" checksum="7C02D71C006912DF" modified="2024-05-29" version="1">MAITMLSYMLFILVLQSPYFARSQSFIGVNYGQLSDNLPAPAVTAKLLQSTAIGKVRLYNADVAIIKSLANTGIGIVIGVANGEIPGLASDPNVAGQWVGSNVLPFYPGSKIEVITVGNEVMTSGDQGLMAQLLPAMQNVQNALNAASLGGKVKVSTVHSMSVLANSDPPSSGSFNSGFSDTMRALLQFQRTTGSPFMINPYPFFAYQSDPRPETLAFCLFQPNAGRVDSASGIKYMNMFDAQVDAVKSALNSMGFKEIEIVVAETGWPYKGDPNEVGPSVDNAKAYNGNLISHLRSKVGTPQMPGKSVDTYIFALYDENLKPGPGSERAFGLFKQDLSMIYDVGLLKTSQTPTTPTTPSPATPAPKAAAWCVPKPGVSDAQLQSNLDYVCGHGLDCSPIQPGGACYEPATILAHAAYAMNLYYQSSGKNPWNCDFARTATLSSTNPSYNGCIYPGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XWU4</accession>
    <name>A0AAF0XWU4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0935188</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH15645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XWU4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04480">
        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="6">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="7">
        <location>
            <begin position="278"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH15645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54135" checksum="E94A0BD684739D7C" modified="2024-05-29" version="1">MAFSYLSELDDHTEDWMIKVRVCRKWESVNTKDGNLISLDMILIDEKGNLMHASVRKHLVSRYADRVLEGRVYNLRNLKVTTNMYPYRPLASNVKLLFLATTAVQELQESDVSIGRYGFEFVNQTVLQSRANDPTVLSDILGCFTGFEGIETVRSGSKKRDIQILTDYSVNSTVTLWGKLGEEFDPALYTQDSGPYVLLVSSVTVKRFRQGSLTFSTTSANKVYINPEVDHVTSIKERFSALSIQAKPIQGTSAAKLTPEEEMFINRMTVDALESVVTLKATITGINSGQGWYYIACRSCVKKAEFENGVYVCRSCGTLEYPLALYRVKAEVQDRTGTTTVVMFNYPAENLLDTSAKKLLGKMKPGDDSVPKELNTLLGKELVFKLKLDKYNLIEGLQDYRVSTVFTPVEGLEAVYAQKEARKVSLKSKQWKKGNIKNQRSSGATALCSLIKFKNIKQGIYNKKKLKRFSIARSAFSSLVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y095</accession>
    <name>A0AAF0Y095_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0935837</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0Y095"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000040">
        <property type="entry name" value="lysine-specific demethylase JMJ25 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF11">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="6">
        <location>
            <begin position="534"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
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        <location>
            <begin position="307"/>
            <end position="339"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
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        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00558"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH16287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="810" mass="93973" checksum="F661077E5B6F5D8F" modified="2024-05-29" version="1">MMKLRERKGCERDERDCERDERDGERKRERPRKVHERDGGKEWAVYKRREMKKSVKEGLRDDEVVVYKRRKVRDGPLSDDDLGGKKMRYIKDLEMESSAQVEKEDGFDGIERDKVLKSNMCHQCQRNDSGRVVECSACEQKRYCEPCMKKWYPKMTEEDFAIKCPVCQFNCNCKRCLRLEVLVKDRERFKVKFSPEEKIRYSRYILSMLLPFLKQFNKEQLMEKRIEAKIKGLPISKIEVQNANCKPDERMFCDNCRTSIADLHRSCPNCNYDLCLSCCSEIRDGCLQGSKEKVSMQFTDPGKDYLHGKKPHPLQSLPAKNSGRGHQKSADEWKSHENGNIPCPPKEMGGCDQGILELRFILGDNYVHDLCKEAENLAKTHKLHVCNTQDQQCSCFNISAGGKKNLLKAACREGSDDNFLYFPTALDLRPGELGHFQCHWLKGEPVIVRNVLATTCGLSWEPMVMWRAFRQIRNRKHDQLLDVAAINCLDWCEVDINVHHFFSGYLNGIYDSDGWPKLLKLKDWPPSSTFEEHLPRHCGEFLNSLPFKEYTDPHAGYLNLAVKLPKDSLKPDMGPKTYIAYGFSQELGRGDSVNVLTHIYEVTPSSDNLSKIETLKQLHDSQDQTEICENEKKTKMKMNGVGSCSDHIQDMKCSDGGALWDIFRRQDAPKLKEYLRKHFNEFRHIYCLPLEQVFDPIHDQTFYLTIEHKNQLKAEYGVEPWTFVQKQGDAVFIPAGCPHQVRNLKSCIKVALDFVSPENVNECIRLTEEFRALPENHRSKEDKLEVKKMTIYAVKQAVKDFSEYAKTKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AEC9</accession>
    <name>A0AAF1AEC9_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">DCAR_0100690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108193682"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36320">
        <property type="entry name" value="OS04G0611300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36320:SF1">
        <property type="entry name" value="OS04G0611300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG81539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="12467" checksum="CF6137CBBC4EDCB9" modified="2024-05-29" version="1">MAKSMRSKRIKRLRAIRREIVAPLNEGKEAAKLAAQEAALAAPKLPVRVSKSAAAAAAAMELITNTSTENADMDVEMAEGTSKSLKPVGGVGKKMKKKVKIGKRSGGKGKIRKKRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AFA6</accession>
    <name>A0AAF1AFA6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="14">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0100323</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="14"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AFA6"/>
    <dbReference type="SMR" id="A0AAF1AFA6"/>
    <dbReference type="KEGG" id="dcr:108205051"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF58">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="16">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="15">
        <location>
            <begin position="206"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG81178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="378" mass="40715" checksum="C7BC4063EC82BB3A" modified="2024-05-29" version="1">MATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIKILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGVGVMMNDRKSRFSVNGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVGLAVAEGAKAAGASRIIGVDIDDRKFERAKKFGVTEFVNPKEHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINKAFDLLHEGDCLRCVLTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AIA5</accession>
    <name>A0AAF1AIA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Early nodulin-93-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0100569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108201761"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005050">
        <property type="entry name" value="Enod93"/>
    </dbReference>
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        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605:SF3">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
        <property type="entry name" value="ENOD93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="12572" checksum="DE8531D3B4E564F6" modified="2024-05-29" version="1">MGIPSEMRDVWAAARKNSILIASPSEDQRLINSRNCTQEGVRAGAKAASIACVVSAVPTLAACRMIPWAKANLNYTAQALIISGASIAAYFITADKTILACARRNAEAEYRKSAQGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJC6</accession>
    <name>A0AAF1AJC6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0101515</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJC6"/>
    <dbReference type="KEGG" id="dcr:108207771"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000690">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
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            <end position="212"/>
        </location>
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            <end position="315"/>
        </location>
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            <begin position="350"/>
            <end position="380"/>
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        </location>
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    </feature>
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        <location>
            <begin position="597"/>
            <end position="692"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="692" mass="78243" checksum="52AE6315CE64B212" modified="2024-05-29" version="1">MTQLVGFLKEGRQEFIDILRRCSKSLKLDQGMQVHGAILRTGYEVDTMINNDLIDMYGKCGKLDLAHKVFDRMVDRNVVTWTSLMCGYLQQGKAVVSLAIFKEMGSSEVNPNEVTLSSNVKACGVLGVVDTGVQIHGLSCKSGFECYLVVGNSLLDMYARCGRIYEAAQVFNEMPYRSLISWNAMISRYAHGAMGDKSLLLFQEMQKQGEIPDEYTFASTLKACSGLGAIREGSQIHAFLITHGFPLLATTIIAGALIELYVKCGYLNQARALYNQVEQKSVILWTSLFLGYAQEANLLEAMDFFRELRNSKIEVDGHVVSSIAGVFADYALVEQGKQIHSYAVKVPEDDISVPNAIVDMYLKCGQIEEAEKLFNDMQEKNLVSWTIMITGYGKYGLGIEAVDLFKRMQLDGIEPDGVTYLALLAACSHSGLVEESREYFSRLCRDRHIKPKVEHYACMVDILGRAGRLNEAKNLIHNMPFNPSTGIWQTLLSSCRVHENLEMGREVERILLNLDGNNPVNYVMVSNLYVGAGYWKESERLRKVAKRKGLKKDAGRSWIEIDKEVHFFFNRDETHPLTRKIHEVLEETERRMRQEIGYTSDVRYALHDVEEETKEESLRVHSEKLAIGLALLCGGMEKGGKPIRIYKNLRVCGDCHEFIKGLSKILSKVFVVRDASRFHKFEDGRCSCNDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJD0</accession>
    <name>A0AAF1AJD0_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LECTIN-LIKE"/>
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    </dbReference>
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        <property type="entry name" value="PP2"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG85469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="178" mass="19480" checksum="DBD844181895B448" modified="2024-05-29" version="1">MAQVQRVRDDIVSLWTQGVQAAKDKSSYKFCTIYFDSEISPLMLINWSSPSLRLSPMDVAELITVCWLQIDGKYNANNLTKGVKYAVYLAVELSDNLCMNGPVTLKLTRPNGTTEEHKEDLKTKPTNTLVGLKVGEFCNTGACGCENTDVKFSMNGCDGTTWKTGLTVVGAVIAPVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALB5</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">DCAR_0206628</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALB5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS01G0855200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG87404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="533" mass="59267" checksum="0F54BD2D5BB4D3C7" modified="2024-05-29" version="1">MRVGSSWLNLSQHLVAYSAVCSPLERRGSGEGFVRYGFGYSSSRIYRSRSFEMGILRGGRSRGGCFASMEAFSDEEFSEKITELALRFQVSDGDGSIRGGVNEADMDSEGNMNRNHNRTGFFRSRLEPVEPPDWQDVISGSIERKANSIELPFSLRMIKKKKKQLKQGLVGVGGSARSSVKKAFSSMVFIIRELQSYTMQMRELLFYEDLEGILVRVQKEMNASFVWLFQQVFSHTPTLMLYVMILLANYSVHSMASNSALAAAPPVSYTMESVLDIADRNDVEKNFDSSSVKIFSVSSSSGKLTSIGGKNGGGGKFPSIASGTNGDEGVDRSASSDYHRTDVPDGPSTSGRSGEQESILGQVSREEELSLWDSVVEEASSMQAVLRDESLDHETMQRFVSPVTAKIESDDYADYLRTELLYQTELLQEPSNTLLLANYAQFLYLVAQDYDRAEDYFKRATNVEPKDAEAVSKYASFLWQARKDLWAAEETYLEAISIDPSNSYYAANYAHFLWTTGGEDTCFPLSSSDANSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ALM2</accession>
    <name>A0AAF1ALM2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(+) kinase</fullName>
            <ecNumber evidence="3">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0103799</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in chlorophyll synthesis and chloroplast protection against oxidative damage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ALM2"/>
    <dbReference type="KEGG" id="dcr:108204394"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000004">
        <property type="entry name" value="NAD kinase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000019">
        <property type="entry name" value="NAD kinase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055214">
        <property type="entry name" value="PTP-NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF6">
        <property type="entry name" value="NAD KINASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22741">
        <property type="entry name" value="PTP-NADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042275547" description="NAD(+) kinase" evidence="17">
        <location>
            <begin position="22"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="DSP-PTPase phosphatase fused to NAD+ Kinase" evidence="18">
        <location>
            <begin position="243"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="414"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="574"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG84615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112833" checksum="E7CEADA76C1DA9EB" modified="2024-05-29" version="1">MAASCSWTCHLLIITTAPSLSIPTPSFNLFLKNTSIPSGFQLRRSRENRSFQRRRFGFVVAAQLTSHFHSATDLDSKSSDISHLPWIGPLPGDIAEVEAYCRIFRASERLHNALMKTLCNPVTGECSVSYGFPSEDKPLLEEKIVSVLGCMICLLNKGREDLLSGRSSLTASFSMSDVNISEDKLPPLASFRTEMKRYCESLHVALEDYLTPDDIERVGIWRKLQRLKNVCYDSGYPRQDGSPCHALFANWTPVYMSTLKEDIESESSEIAFYRGSQLTEESLKWLLVNGFKTIVDLRAEAVKDHFYQAMLDEAVFTQKVELVKLPVEVGTAPTMEQVEKFSSLVSDSRKKPIYLHSKEGVWRTSAMVSRWRQYMTHYRSPSPSNKAVLPNGIMLGGTTDMKNMQMSVKLKEDALDDKNGSMEVGPKTRYSSIGKLPKKDSQAPDESNQSDNGISGSLDTMSITPEVDVYKNESLIDDWSAVKPFDSQLPPSNVFSRKSMVRFFKNKKISPGRYFNNNHKRWMELCGSRGNYNGTILSSKSSQTNVKLVDQQRETSNGSAISYVNPGTNGTYKEGGSYTYQSSDPISTAPSDKMYTTRKSDDRISATNGLDKSAISSTMSSDQRNKTTATVSSQNDELGLIEGNMCASATGVVRVQSRKKAEMFLVRTDGVSCSREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGEELMEEAKEVASFLYHQQKMNVLVEPELHDIFARIPGFGFVQTFYSQDTSDLHERVDFVACLGGDGVILHGSNLFRGAVPPFVSFNLGSLGFLTSHIFADYKQDLKQVIHGNNTMDGVYITLRMRLRCEIFRNGKSVPGKVFDVLNEIVVDRGSNPYLSKIECYEHDRLITKVQGDGVIIATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPKDARSNAWVSFDGKRRQQLSRGDSVRINMSQHPLPTVNKCDQTGDWFRSLIRCLNWNERLDQKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMD9</accession>
    <name>A0AAF1AMD9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0207779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>gibberellin A12 + 2 2-oxoglutarate + 3 O2 + H(+) = gibberellin A9 + 2 succinate + 3 CO2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:60772"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
            <dbReference type="ChEBI" id="CHEBI:73255"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:60773"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMD9"/>
    <dbReference type="KEGG" id="dcr:108209173"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045544">
        <property type="term" value="F:gibberellin 20-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000003">
        <property type="entry name" value="Gibberellin 20 oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="224"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="380" mass="43160" checksum="AC5BB55C8B9B57A6" modified="2024-05-29" version="1">MPMPSPTLKSWYSPTKESKNEQKALVFDASLLQHELNIPAQFIWPDHEKPSADQPQTPPLVVPPIDLKGFLTGEPDAISNAIKLVDEACRKHGFFQVVNHGIDSKLINEAHKNISGFFGLPLLEKQRAQKKAGDVSGYASSFTNRFSSKLPWKETLSFRYSADQQSAYKVEDYFSSVMGEEFRQFGRICQEYCEAMSNLSLVIMELLGMSLGIGPSYLREFFEENDSIMRLNFYPPCQKPDQTLGTGPHCDPTSLTILHQDDVGGLEVYVDNQWRSILPNTEAFVVNIGDTFMALSNGIYKSCLHRAVVNNRTPRKSLAFFLSPKMDKVVRSPEALIKMDGARNFPDFTWSTFLEFTQKHYRSDMNTLEAFADWLKLRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AMF5</accession>
    <name>A0AAF1AMF5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0105055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AMF5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF135">
        <property type="entry name" value="OS02G0640800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="84" mass="9237" checksum="99535866620ECEC6" modified="2024-05-29" version="1">MFDPDAVDEEGELMSIPILDCWQKLPNMLINCVHPGYTQTELTSKTGPLTPEEGARAPVMLALLPDGGPSGTYFLEMQHQLSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANA5</accession>
    <name>A0AAF1ANA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0208697</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ANA5"/>
    <dbReference type="KEGG" id="dcr:108207737"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000173">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF35">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="11"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG89459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="343" mass="38406" checksum="38926C01A4E3C297" modified="2024-05-29" version="1">MLELAVATENFSAENLLGEGGFGYVYKGVLPNERKIAVKRLKLGSRQGEREFQAELETISRVHHRHLVSMIGYCIAGTERLLVYEFVENSTLEYHLHGKDSPVMDWGTRMRIAIGSAKGLAYLHEDCNPTIIHRDIKSANILLNSKFEAKVADFGLAKFFTDTNHDITHVTTRVVGTFGYLAPEYASSGRASHKSDVFSYGVILLELITGRKPLSKDFSSTDFLVPWAKPLLRLAMENNKFNTLVDPRLGNNCNYDEIAIMASCAAACVRNSEQLRPRMSQILRTLEGDVSPMELFSDGTRPGYSSFHDVFDILHLGSGQTGNTSDPQTRLLRRSGTNYHRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQG0</accession>
    <name>A0AAF1AQG0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0310672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQG0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF237">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="348"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG91423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="429" mass="48959" checksum="697D8F1489C0E467" modified="2024-05-29" version="1">MDCIEKDMISELSCPLQETILGFLPIRDAVRSSALSRKWRHKWTMIPHLIFDDQFFDSKRKNLRQYQDKKLRAHGFVSVINKVLLLHQGPILEFSLTNLEGCDTKIVHDYISQWLPLLSRNGIKKFNLKNCHFDNIAAYGFSSLDLTQLRLDGVLFSTSPSFRSFTCLRSLELVDCINNKPSIFVCPVLGELVLIYCEGLLPNNFRAPNLKRLKQAICKLPLEYSLAGLPNLKEFSCTALTIPMINAKAFSGVNFLDSLHTIEKLSLKFTFVKYLVAGGCPVKLSKLMPHLKILLLSGMELIHLSDITCLLCLMQSAPNLKKLHISAMEINDAVEGNLRNYLAKKFVDCTFEHLEIVTCTWLRGLRDELELVKFLLANSPLLKKMFIHHCVTVRTDVALNIAEEMLQYARASSEAQIRFLKDDLNIEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ARA5</accession>
    <name>A0AAF1ARA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0208653</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG89415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ARA5"/>
    <dbReference type="KEGG" id="dcr:108205637"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042631">
        <property type="term" value="P:cellular response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902457">
        <property type="term" value="P:negative regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000059">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="5"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG89415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="27019" checksum="ACD08C26F7CDF9E2" modified="2024-05-29" version="1">MAEKACVKRLQKEYRALCKEPAPQCVARPSSNNILEWYYVLEGSAGTPFAGGYYFGKIKFPPEYPFKPPGISMTTPNGRFKTQTKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVTTTVAEKQRLAKSSLAFNCKNVKFRNLFPEYVEKYEKEQQLFEKVVPEQKRAEHASEEVRPLLEQRGQSIGEGNKPEPQQDIKNVRRQSFPTWVLVLLVSIFGIVISLPLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVC2</accession>
    <name>A0AAF1AVC2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0415634</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVC2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000504">
        <property type="entry name" value="ABC transporter G family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="620"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="42"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG96299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="703" mass="78820" checksum="DA56AA76195C3193" modified="2024-05-29" version="1">MEIEGHMAQGDRMVVGGLSPLSETLWKERANTEFIGDVSARLSWKDLTVMVTLSNGETQNVLEGLTGFAEPGTFTALMGPSGSGKSTLLDSLASRLAANAFLSGNIYLNGRKQKLSYGTAAYVTQDDNLIGTLTVRETISYSARLRLPDKMAWEEKRSLVESTIVEMGLQDCAETVIGNWHLRGISGGEKRRVSIALEILMRPRLLFLDEPTSGLDSASAFFVTQTLRALSRDGRTVIASIHQPSSEVFELFDRLYLLSGGKTVYFGQASEAYEFFAQAGFPCPSLRNPSDHFLRCINSDFDKVKATLKGSMKLRFEANDDPLDKTTTAEATRTLTEFYRSSHYFYSAREKVEAMSKLKGTVLDSGGSQASFLMQAFTLTKRSFVNMSRDFGYYWLRLVIYIVVTVCIGTIYLNVGTSYNSILARGACASFVFGFVTFMSIGGFPSFVEDMKVFQRERLNGHYGVVAFVISNTLSAMPFLLMITFISGTICYFMVHLHSGFTHYLFFVLCLYASVTVVESLMMAIASIVPNFLMGIIIGAGIQGIFMLVSGYFRLPHDIPKPVWRYPMSYISFHFWALQGQYQNDLKGLVFDNQTPDQPKIPGEYVLEYVFQIDVNRSKWVDLSVIFSMIVIYRIIFFLMIKINEDLTPWLRGYMARRRIMQKNGGQNTTVAPYGLTQSPSLRNYVDNIATGGGRRRQPPRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVG1</accession>
    <name>A0AAF1AVG1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0313918</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
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        <property type="protein sequence ID" value="WOG94622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVG1"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
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        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
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        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF245">
        <property type="entry name" value="(-)-ALPHA-TERPINEOL SYNTHASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Terpenoid synthases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="7">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="8">
        <location>
            <begin position="204"/>
            <end position="444"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG94622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="501" mass="58099" checksum="ABF51DE8CD4BD0E3" modified="2024-05-29" version="1">MSTLTVNHPLPVVRRSGNYKPCIWDDDFVQTLATDYTKERFNGRVNELKVNVIEMLNDVAKPNLAQLELIDDLQRLGLGYHFEQEIKSVLMKIYEDQSSETLERSDLHAAALRFRLLRQHGYRISQGLIFFCNITDPNLSIKVRYALELPVNWRMLRLEARWYIDFYSRSNNMNPELLELAKLDYNTVQGLYQEELKIASRWWKELDMSTKLSFARDRLVESFIWTVGIAPQPQFGNCRIEAAKAIQMISVIDDIYDIYGTVHELELFTDAIERWDINSVQLLPDYMRICFLALYNMVNETVHHYIHREQNTDVLPHLKKAWFELVKCYLVEARWYHSGYTPTLEEYLENGKLSIALPSLATQSYIFLANPIEKEALEFLEDMPDILHSLGTVCRIVDDNGTSSDEIARGDVPKSIQCHMFHNGTSEEAARAEMRALMRKKWREINACRARDMPLSSPNIETILNIGRSGHYVYYDSDGYAGQDGKSKAMFLSLVVQPVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVI5</accession>
    <name>A0AAF1AVI5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0416297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVI5"/>
    <dbReference type="KEGG" id="dcr:108218667"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044217">
        <property type="entry name" value="CLPT1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47016">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPT1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47016:SF1">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPT1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="2">
        <location>
            <begin position="84"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG96958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="26244" checksum="93DB5F8CA0F0B74D" modified="2024-05-29" version="1">MAAHALSLLPISTPSRNHFCCKQESIKSSLCYHLSLASSFVGAKLAVNYTNSRQYTHTQHSSTVATISVSLPTGTPDRISRQKSPKWSARAIKSYAMAELEARKLKYPNTGTEALLMGILVEGTSLAAKFLRENGITLFKVRDETVKLLGKSDMYFFSPEHPPFTEPAQRALDWAIAEKLKSGEDGEVTAAHFLLGVWSEKESAGHKIMAALGFDDEKAKEVAKFMDKDIDLGFKTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVK0</accession>
    <name>A0AAF1AVK0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Fused homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0416342</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219192"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14002">
        <property type="entry name" value="STKc_STK36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000292">
        <property type="entry name" value="Serine/threonine-protein kinase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000223">
        <property type="entry name" value="Serine/threonine-protein kinase TIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983">
        <property type="entry name" value="PROTEIN KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG97003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1353" mass="147814" checksum="AFC954268A1F0B5A" modified="2024-05-29" version="1">MGVENYHVIELVGEGSFGKVYKGRRKYTGQTVAMKFIMKHGKTDKDIQNLRQEIEILRKLKHENIIGMLDSFESPQEFCVVTEFAQGELFEILEDDKCLPEEQVQAIAKQLVRALHYLHSNRIIHRDMKPQNILIGAGSVVKLCDFGFARAMSTNTVVLRSIKGTPLYMAPELVREQPYNHTADLWSLGVILYELFVGQPPFYTNSVYALIRHIIKDPVKYPENMSSNFKSFLRGLLNKVPQSRLTWPALLEHPFVRETSEDIEAREIRATTAAARGYDASWKGEGDLQKAELPLSSNESRNPQSLPSTENGDLHSLQQDIQSHTPSSALGNSSPKDSPGVTSYEEDVQSGCQVLDRLENNSRTVKGANLIGQDNEALTVILLRLKSVCTSSPDSLRDQDLICFNQSLRILSNLVAASAINYSVLNEIISELLSATASLANLKLADSSDLIAKSLLLIKKLIDNYGGTIGGSYVKHWVSLVELFSKVVGSADDSSGRLLYESTACITVVLSKAAQSLKTSALAVTSEVVSGPSAIIEIVKQILDHAKVSNVVELLCLCLANCGSSLISGSSNLLRAACEACRSLWSLIDALELLYVKENTHLFPLCSLRSHSLLRLDIRNHEQGSLIGVESTIVIDAVTRSFLRSKPIQVAVYYCLRQRAAEPVLFAGTQLILRCCLHSSSISSILCGLPSSLPATKIVSGGGDGTIVSEIFSILSLCASSYRDAQTADTNNLKCKIANPGILVVHSCLVLATVAQCLKSSGRNSPVFMLTSIPKKQLSRLSVLAHHISLDDRTQNSFQPRCSSSMLALASIMSLETGVLSESSISEIAVPLIPRTATLCDNLKILLADENGMVPAIQNGMLSYWHGFRDGSVGLLEARLKWGGALAVQQLCASGIPQLLITLLANSLSDVSVQGSDFTKDQIALSPVGVVWTISSICQCLSGGALTFRQILLKSEHVKFISDLISDVHLNLLRCWTGPGGGKGGVRDTINAVIDLLAFPFVAIQSAPGLASATASVNGGFVLNISSPGGRVCAEDKDMVKAIEASLGKYIQIILEVGVPGYILRCLEHMELKDSARLVAFLAKMTGHRPLVVQLIGKGLLDPARMRRLFDRSSPREVTMDVLMIVSDLARMDKVFYEHLDGAEILQFLKDFLTHEDPNVRAKTCSAIGNMFRHSSYFYSLMAKQQIVNLLIDRCSDPDKRTRKFACFAIGNAAYHNDLLYDELKRSIPQLANLLLSAEEDKTKANAAGALSNLVRNSNKLCEDIVSKGAIQALLQLVADCSLVALNPNRRDAINESPLKIAMFSLAKMCAHMPCRQFMRSSELYPVIGRLRQSQEPTISNYASVILSKTSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AWW0</accession>
    <name>A0AAF1AWW0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0414765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AWW0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144:SF16">
        <property type="entry name" value="OS02G0129000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144">
        <property type="entry name" value="OS10G0409366 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="141" mass="16850" checksum="D771498E13C927E4" modified="2024-05-29" version="1">MEEKYFFLRVSHKGQFLKTKYHGGSCLKVPTAMDPEKFSYSVLMEYPKKTVKPQKYIIPEEGKDWIMKTIDESWRVYKSRFKAHYYTKYDTDEDRLKNRPQHISLEQFKSLIAYWGDEFFPKVKSSCFILVLSHNVLSVIS</sequence>
</entry>
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    <name>A0AAF1AY42_DAUCS</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GASA family.</text>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99628.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13317" checksum="F4A74FBE6CD5B28D" modified="2024-05-29" version="1">MRPALATSTSRLITTPANVLRCFHKSSMSVYILSLLVLVLTLMYCCIKIKANSRSTNIYTINCTASCDTRCSETKRPNLCKRACGSCCGKCNCVPPGTSGNYEACPCYFNLKTHNNTRKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF1B2F1_DAUCS</name>
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        <name evidence="7" type="primary">LSM1</name>
        <name evidence="9" type="ORF">DCAR_0623490</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation by promoting decapping and leading to accurate 5'-3' mRNA decay. LSM1A and LSM1B are essential for the formation of the cytoplasmic LSM1-LSM7 complex which regulates developmental gene expression by the decapping of specific development-related transcripts. Required for P-body formation during heat stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="7">P-body</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sm"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LSM1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF20">
        <property type="entry name" value="SM-LIKE PROTEIN LSM1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
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        <location>
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            <end position="85"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="14518" checksum="4AA5E3A668A0658B" modified="2024-05-29" version="1">MSWAGPEDFYLSTSLASYLDKKLLVLLRDGRKLLGILRSFDQFANAVLEGACERVIVGEIYCDIPLGLYIIRGENVVLIGELDLEKDELPPHMTRVSPAEIKRAQKAEREASDLKGTMRKRMEFLDMD</sequence>
</entry>
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            <fullName evidence="7">LisH domain-containing protein</fullName>
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        <name evidence="5" type="ORF">DCAR_0521238</name>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2S6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044716">
        <property type="entry name" value="LEUNIG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
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    </dbReference>
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        <location>
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</entry>
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        <lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
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    </reference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="298" mass="33576" checksum="1D8B03155EC4DC17" modified="2024-05-29" version="1">MAAAIASLCFSALTSQSASERKLSAPSLISNFDASRLRSSHDSVAVRVSQSSSSARFVVRCMASASTDVPTVSETKANFLQAYKRPIPSIYNTVLQELIVQQHLTRYKRTHRYDAVFALGFVTVYDRLMEGYPSDEDKEAIFRAYIKALKEDPEQYRSDAQKLEEWARSQTANTLVDFSTKEGDVESILKDISERARSKESFSYSRFFAIGLFRLLELADATEPTTLEKLCAALNIDKRSVDRDLAVYRNLLTKLVQAKELLKEYVEREKKKAEERTGPQTANEAVANCLGEYLYLGL</sequence>
</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF88">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH02437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25518" checksum="554CB746E496E026" modified="2024-05-29" version="1">MKNAELIFIPAPVRGHLISMVELAKLLISRNQSLSVTIFIMKFPYDTGVNSYIESLETNPIPRLAILEVPLSSSEAYKFESYYTMIPTFIDSHVTIVKNAVMAQPDRAVAALVLDVFCVSMIDMAKELNIPSYVYFTSGASYLGVLFHLQGLTDYENEDIYEYKDSDAELSVCCFNNRVPAKVLPAICLDEKGSQVMLSCGRKFRETIRDYCEHVCGAGNVCCSVSCGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B7F5</accession>
    <name>A0AAF1B7F5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B7F5"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="120"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH06637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="216" mass="23775" checksum="7F1E9F0E46F070D1" modified="2024-05-29" version="1">MADYYWRHTAGRQPDLPPLLELRPCLNSDVRGAQDYNGSDGRRGAQRVMRAEDSLRASSDYCSCGESVCQILCFNSRSEVTIPIDDHSIMGSGGSNPAMAELQLVGGSGPEGLLPPNASCTLFVDGLPQDCSPREVAHIFRPLRGYKGVRFVPRASRNSNDDPLVYCFVDFESPGYAANALYCLQGYEFDEQDQDDVRLRLGFAHFVQRPGATSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B8R2</accession>
    <name>A0AAF1B8R2_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0727499</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="182"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
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        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
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        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
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            <end position="48"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
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            <end position="104"/>
        </location>
    </feature>
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            <end position="534"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH08062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="534" mass="59110" checksum="8A559ADF089761B3" modified="2024-05-29" version="1">MGCFRVSKGKKKRSEQNMYVKRVNPQDHPPTALPEPPTQTRSLQSAPSSFRDRVKQQPNSSTNSRARALSAPSSLDTAEQDALSVDYEEQEGSKSRFGSMKERQTPNPIPLPLPAPQNASVMKKLGSFKVMNASGQHNISGPLPLPPALPPSIPSTSPQTLPIKGKVRNFSYEELATACNNFSAERCLSVGLSSTIYKASFGDDKHGSRKLDATVTRFKSSTQGQKEFINEVNTLASLQHPSICKLLGFHAHEGSEWRMLVYERLYHGSLDLLLYGKSDHPLLDWNARMKVASCAAQGLTFLHDEGPFQAMFHEFSTRNIQIDKDFSAKLSGYGCISHIPETYISSTSVARTHPSVEIPEKGLLTPKSNVWSFGVVLLELLTGRRNLDIHQAKEEMNLVKWSWPYLSDDGKLSLIMDPQLKGWFPASAARQLADITQRCLQTDPSERPTMRTVAEHLKMIQQMKFSSRLPLQEPGSHVPRNMMRSPSLNGIITPGPQLSLSPSLTTRLSASQTSPLGIPHFMSPQNLSTVSGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCQ5</accession>
    <name>A0AAF1BCQ5_DAUCS</name>
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        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">DCAR_0934075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36055">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36055:SF1">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
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        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="702"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH14555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="715" mass="78829" checksum="5B5B31750B27049B" modified="2024-05-29" version="1">MPGCHCRSRAGRNLDAMIVGVGNDVQDALVRQTIGIGPYLSFPRVNDVPMQWVQLLNGFDQQELYGWPMLTPLKIQMQKCDKCSLEFCSTVNYRRHIRVHRRLKVDKDSYKSRDSLGAFWDKVSLDEAKNILSFKDVTLEGLPGSSIIKALASFIRAPGFCTLPQVYVKAGLVLLDAIEATPSRLPISSQELFTVLDDASERTFLSGSTEGSVQKFIFDGEARKVGLEIKNLVACTSFLIEQSLVKAWIADKDAEALRCQKLLVEEEEAAQRRQAELLERKRLRKLRQKEQKTKGRSCKETTVLTSAADILETVPSAESSTPPQSEADLHAVSSPPQSEVDLHSSSSPPQSEGDLHTSSSLPHSEGDLHELEDHSSVIEPVHIFNNEVNGHINIEGGYNSELVDIGRAKNVELPESQDSNNQQIVDSKLQVLESKSEQNACHPSHSLDVVKPDPIQAQAPPNDLMVAPVNGSDISTGKGTVENGRECVSALLHEREISQTDHTSCEVMIGSITVTLRNCTTQKQHDDSQSESLHENCSIDHVTCENVGAHYKQIKPESTMNNTLISNDRHEVESEFVVQTGVTGVGTMPDKCSHASSEVDSDSKQNDFIQPAKVALSSHSARAFLAHRWKEAISEDHETLVLSQEAESQGHCDAINECSEAVSTVKAIDSEELSVLGRAKNFLDIVGAPEYPTSRPVDSKRTSKPRKGYQMKVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BEB2</accession>
    <name>A0AAF1BEB2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0936182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BEB2"/>
    <dbReference type="KEGG" id="dcr:108202954"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002166">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001636">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g20540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF453">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="58355" checksum="38240E42A234160C" modified="2024-05-29" version="1">MLAVPTLNKHIVTLLNKSNHLNHLKQIQAFLVTLGYSQTQFYAFKLVRFCNLSLFNLNYARSIFDNLRFRNIYLYTAMITAYASQPDHYSALNLYIQMFRKANPGPNEFIFPHVLRACAGVLGPFGTESLHTQIAKLGFGEYPVVQTALVDSYSRFCSDLGVARKVFDEMTERNVVSWTAMVSGYTRVGEMGNAVLLFEEMPERDSPSWNAVIAGCTQNGLFSEAISLFKRMVGSSNRPNQVTVVCTLSACGHNGMLQLGKCIHGYIYRNDLGSDCYVSNGLVDMYGKCGSLVEAKRVFDKTSKKNLTSWNSMINCLALHGQSDCAVSIFEEMLGHRDNVLPDSVTFVGLLNACTHGGLVEQGRKYFSMMINDYGLEPHIEHYGCLIDLLGRAGQFEEAKEVIREMKIPPDEVVWGSLLNGCKIHGQMDMAEYAVKKLIEIDPNNGGYGIMLANIYSESGSWNEVRKVRKMLKEQNAHKTPGCSWIEIDNQVHQFYSVDKTHPRTEDIYLTLECLGNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BF68</accession>
    <name>A0AAF1BF68_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BF68"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328">
        <property type="entry name" value="FAR-RED IMPAIRED RESPONSIVE (FAR1) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46328:SF35">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH14722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="167" mass="19350" checksum="8822D208BA26E6AF" modified="2024-05-29" version="1">MSEPFDVEEEDNDDDEDNNEEISEKLSPPEVNSVFDSEVEVCEYYKRYGKQNGYGNSHRKVVDSLNPKPVGKSNCKVRLCAKRMDDGKWCVTQFVNEHNHDLSPSKAHRFRCNRKLTNNAKRSLSLYDEAGIPINKSYITLVVEHGGHENMSFNKKDCENYTRTIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BFQ4</accession>
    <name>A0AAF1BFQ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0935682</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108202681"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059143">
        <property type="entry name" value="NFP_LysM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059144">
        <property type="entry name" value="NFP_LysM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF15">
        <property type="entry name" value="SERINE_THREONINE RECEPTOR-LIKE KINASE NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23457">
        <property type="entry name" value="LysM2_NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23462">
        <property type="entry name" value="LysM3_NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042126765" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="13">
        <location>
            <begin position="181"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="316"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH16133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="68480" checksum="42C0E694A663DB4A" modified="2024-05-29" version="1">MKNRVLCFLNTLFLFILSCDSQAVIEPTTSGYTCSLNQTISPCQTYVYYRAASPDYLDLASVGDLFSVSRLMIANPSNISSVSSPLVPSQSLLVPIQCSCNRINSSLSISYAGLNYTIKKDNTFYLVSSIQFQNLTSYQSVEVVNPSLVPTNLSIGENVIFPIFCKCPDKAQVENQTKYLISYVFQPFDNFSSIARKFGSTTQAIIDVNGNNIKTFQTVFVPVSRLPVFTQPVVAAAAPTAPVSVKTEENKDKVRGLSVGLGICGLLLLLVCGLWGYRETSMKKKRGKYKDEEKPQVIQKKKAVDVDLMDDVSDCLDKYRVFGIDELVEATEGFDEKWHVQGSVYKGYINGILYAIKKMKWNACEELKILQKVNHGNLVKLEGFCIDPEDGNCYLIYEYVENGSLHSWLHSSRSEQLTWKMRLRVAIDVANGLQYIHEHTQPRVVHKDIKSSNILLDGHMRAKIANFGLAKSGCNAITMHIVGTQGYIAPEYLTDGVVSTKMDVFSFGVVLLELVSGREAINEEGKVLWSTVDGILEKKDGKMERLIEWMDDSLRTGESNLLDSVANVVSVAIACLHRDPSRRPSMVDIVYALCKSDDLFSDLSQDFSPGMILAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X5E2</accession>
    <name>A0A161X5E2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X5E2"/>
    <dbReference type="Gramene" id="KZN06145">
        <property type="protein sequence ID" value="KZN06145"/>
        <property type="gene ID" value="DCAR_006982"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205580"/>
    <dbReference type="OMA" id="RIMIFTM"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="473" mass="54047" checksum="6B5147DC328E2EDB" modified="2016-07-06" version="1">MISFTLFALIIVAIFILLRLFLIRTALISIVNKCLHCIEDAVHVYQFFKVPEFNENHQENQFYRKVLTYVNSLSSIEDSDFTNLFSGSKSNDIILCLDDNQVVFDTFLGARVSWKNEIDSISRKRTFVLKIKKKDKRRVLQSYLQHIHTVSDEIDQRWREIRLFINTELNQRWRSVPLAHPVTIDTMVMDLDLKNRIKSDVEAFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVVAMAKLLNYDVYDIDLSKVSDDSDLKLLLLQTRPKSLIVVEDLDRFLSDSSAESSVSLSGVLNFMDGIVNSCTGDEKVMVFTMNCKDLIDPVVLRPGRVDVHIYFPLCNFNAFKTLANSYLGVKDHKLFPHVEEIFETGATMSPAEIAELMVMNRSSPSRALKSVITALQISGDARAGKSGQRLSESKSIDEGSMLSWKDVSSPAIKEVRKLYNVLRLKKSGPSDHQLHHNLSLTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YI76</accession>
    <name>A0A161YI76_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0518496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YI76"/>
    <dbReference type="Gramene" id="KZM92909">
        <property type="protein sequence ID" value="KZM92909"/>
        <property type="gene ID" value="DCAR_016154"/>
    </dbReference>
    <dbReference type="OMA" id="KGNPPDA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36755:SF1">
        <property type="entry name" value="OS06G0149300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36755">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG99148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="62" mass="5837" checksum="FF107131C96B4001" modified="2016-07-06" version="1">MTIKPTVALRAIVVGGVAAFAKIASVAKAAGGVKLGAAAAAMTAAATATVAGSKQDQTNAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YL70</accession>
    <name>A0A161YL70_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="WOH00660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="379" mass="43066" checksum="257F55500101E86D" modified="2016-07-06" version="1">MTSNSSLIGDILTDDLVTEILHRLPVKSLLRFKLVSKPWLSLISSPRFIKSHLHHALTSPAAHQTLIVYKKHHLSISLFQLRSPQIGPSLGVPYSRGEFSFTPFTMLVASQNGVVCVAVADFPRHEMALSNLYKLNNCCLYLWNPATRQSRVLPPHDIREDVMSVCFGFGFDSVGNEFKVVRVVSSFRKPFSAEVYSERKDAWRRVKPKPCDVPYYEVFDVCVNGLLCCTGMYGLMAFDLNKEVFRSGIRIPVRRRDIKRFNARVIVVNESVAVGFFSRDMELSGKVKVWTLDDDACLRGDQVEASWTLVLSIRVDIPGRFVRGYCSSKDLLLVIGEDIWLSYNTEEKEVKPFPDSIYLSQVIKYNESLISIRGSKLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">UspA domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_022493</name>
        <name evidence="4" type="ORF">DCAR_0623664</name>
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    <organism evidence="3">
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        <lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UNIVERSAL STRESS PROTEIN PHOS32"/>
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        <location>
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            <end position="45"/>
        </location>
    </feature>
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        <location>
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            <end position="239"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM90142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH04255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="26170" checksum="081970425D211CA5" modified="2016-07-06" version="1">MDSQKPAPSSPDSDLPLLAAIKVKASSPRYRPSSTPHATDTPTSAAQRRIGIAVDLSDESAFAVKWSVHHYLRPGDAVILLHVRPTSVLYGADWGSVDSDTDEATKQKLETEFDDFTTSKAADLSQPLVDAKIPYKIHIVKDHDMRERLCLEVERLGLSAVIMGSRGFGATKNGGNERLGSVSDYCVRHCVCPVVVVRYPDEKDRNVGGGDKKKDVVAKNEVEVENRNADEDDKEKKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5I7</accession>
    <name>A0A162A5I7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_018942</name>
        <name evidence="5" type="ORF">DCAR_0521667</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A5I7"/>
    <dbReference type="STRING" id="79200.A0A162A5I7"/>
    <dbReference type="ESTHER" id="daucs-a0a162a5i7">
        <property type="family name" value="Plant_carboxylesterase"/>
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    <dbReference type="Gramene" id="KZM95700">
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        <property type="gene ID" value="DCAR_018942"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
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        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF635">
        <property type="entry name" value="OS07G0162700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="69"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM95700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH02278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="308" mass="34097" checksum="F7DCE25CB76AE5FE" modified="2016-07-06" version="1">MALVAEAPGYIQVFSDGSVKRFAPDIIAPSVNPTHGFMSKDVVIDSSKPITGRVFVPENISSKNAVPVLVYFHGGGFSIGSTTWSGYHYFLGGLSVTCKCVILSVDYRLAPEYKLPIAYEDCYSSLAWLCNAVSHEPWLQKADLSRVFLSGDSAGGNIAHHVAVKAIRDKACPIRVKGILPVHPYFGSEKRTKLEQETGDVGEVAMNDFFWGLSLPEGSNRDFYGCNFENGDLSKDEWARFPAVVVYVGGLDFLKERGVMYVEFLQKRIKGVKLVEAEEDTHVYHIFYPDSDASHLLQGLMSEFINSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A953</accession>
    <name>A0A162A953_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_014756</name>
        <name evidence="4" type="ORF">DCAR_0416122</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A953"/>
    <dbReference type="STRING" id="79200.A0A162A953"/>
    <dbReference type="Gramene" id="KZM97882">
        <property type="protein sequence ID" value="KZM97882"/>
        <property type="gene ID" value="DCAR_014756"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108216071"/>
    <dbReference type="OMA" id="NTHTHLW"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF6">
        <property type="entry name" value="CLASS I PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="2">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM97882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="235" mass="25454" checksum="97A2D23EE1CBAA75" modified="2016-07-06" version="1">MSQTLFKTFPKLAHIAITCKNGAVFAPSSSNFYLPRTSITRTICCYSSSSEKGSNFEAFGRELLGLSDEQLLSQCDQTTFKSSGPGGQHRNKRETAVRLKHLSTGIVAQAAEDRSQHMNRASALTRLRTLLALKVRNTIDLDTYSPPPELLQILPAKSTIRGKDIGSQIGPKNPKFVLGMQALLDLICAVDGSVADAAKKLGLSTGALSRLILSDDSLRMAVNEFRGSKGMKPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AE52</accession>
    <name>A0A162AE52_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_012816</name>
        <name evidence="8" type="ORF">DCAR_0418300</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162AE52"/>
    <dbReference type="STRING" id="79200.A0A162AE52"/>
    <dbReference type="Gramene" id="KZM99822">
        <property type="protein sequence ID" value="KZM99822"/>
        <property type="gene ID" value="DCAR_012816"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219353"/>
    <dbReference type="OMA" id="WIPAPRE"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001188">
        <property type="entry name" value="Transducin/WD40 repeat-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="39"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="99"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="310"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="397"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM99822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG98954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="49760" checksum="99B60971992349DD" modified="2016-07-06" version="1">MWKQVRERETGVINSKLFTRRVKSDRVSSLQLSNHKEIVSAHRGAINSLQVDLTEGRYLLSGGSDASVAVYDVQRATEYVAGGLVAKHKNILLVDKQHEHGHKYAVSSAIWYPVDTGLFVTGSYDHFVNVWDTNTTQVVMNFKMPGKVYKTAMSALSTSHMLIAVGSEDVQVRLCDIASGAFSHTLSGHRDGVMSLEWSTSSEWVLLTGGRDGAIRFWDIRRAGCFSVLNQSHTQLGRRPPFLQRVSTNKASTSKSSLASVSSKSKTSQRKHVNGNGSKQSSAAKKNVIQEKASAGKRLHPGLISSRDRSTAHYGAVIGLKMTSDGQYLLSAGSDSRLRLWDVESCCNTLVNFDIVRLQTSKPMQLAVSPDPDLVFVPCMSTVKAFSMWSGQTMLSFRNHYDNVNSCWYNEQDQELYTGASDRQILVWSPFKSNLEHEGVAPDQDNWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ASU4</accession>
    <name>A0A162ASU4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0207051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162ASU4"/>
    <dbReference type="Gramene" id="KZN05413">
        <property type="protein sequence ID" value="KZN05413"/>
        <property type="gene ID" value="DCAR_006250"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108207537"/>
    <dbReference type="OMA" id="CARDCKL"/>
    <dbReference type="OrthoDB" id="1703246at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF93">
        <property type="entry name" value="PECTIN ACETYLESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042620664" description="Pectin acetylesterase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG87819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="398" mass="44439" checksum="2921F0924FB5FC60" modified="2016-07-06" version="1">MAGRRLSQAFYTLLVVCNLIKFGRGVDFPYVTRLVNLTYLHYAVRQGAVCLDGSPPAYQFDKGFGDGVNNWLVFLEGGGWCDTTEDCLNRILLNGMGSSTNMFPKNFTGMTSNEPSLNPYFYNWNRVYVRYCDGASFTGNNVDPVHKLHYKGAKIFNVIVKDLLAKGMKNASNALLTGCSAGGLAATLNCDKFRSLLSTSTRVKCASDAGYFPHLKDVLGGFSFAKYFDNIVKLHGSAKNLPKSCTSKMKPRSLCFHPQYSLPYVQTPVFIINSAYDTYQIANILAPNITNLPKSWETCRSNTTTCLTPDHVETLKEFRLGFLDALPKLGNSSFRGMFITSCLIHCQYHLQQSWHGDDPRFRLHNKAISEALGEWYSGKAALQIIDHENDLPRVCLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SKJ5</accession>
    <name>A0A164SKJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_023378</name>
        <name evidence="15" type="ORF">DCAR_0726822</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SKJ5"/>
    <dbReference type="SMR" id="A0A164SKJ5"/>
    <dbReference type="Gramene" id="KZM86244">
        <property type="protein sequence ID" value="KZM86244"/>
        <property type="gene ID" value="DCAR_023378"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108193878"/>
    <dbReference type="OMA" id="EIRIHDS"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000004">
        <property type="entry name" value="Probable protein phosphatase 2C 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF620">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="74"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZM86244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH07392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="381" mass="41310" checksum="9D3AAAAFAC1D57F0" modified="2016-07-06" version="1">MVAEAEILLCGDYKSDVVSIRPDLFDPSKTGLDVCASGSVSVQVTRSESAVRCSTIIQATSLESGASDYVPSIRSGSHTDIGPRRSNEDEHICVDDICAWSGSRYSLPLPSSFFAVFDGHGGSEASAYLKNNAMRLFFEDVALPQIYDIDDILLEDLENSHCKAFLLADQALAAEGSVCDFCGTTALTALVLGRHLLVANVGDSRAVLCRKGVAVPMSQDHRPSYISELKRIKELGGYIEDGYLNGELAVTRALGDWYMKSPSGCPAPLTAEPDVQRTLLTKDDEFLIIACDGIWDVMSNEEAVRLVRGQLRQHDDPQKCAKALVGQALRLDTSDNLTAIIVCFSSPAELTESVTSQRPKLSCCSLSDDARNRLRSFLQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SRF3</accession>
    <name>A0A164SRF3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0727137</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </subcellularLocation>
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        <property type="entry name" value="Znf_B-box"/>
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        <property type="entry name" value="CCT MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31874:SF1">
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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    </evidence>
    <sequence length="417" mass="47532" checksum="6BBB4CDB079270A5" modified="2016-07-06" version="1">MESESSIANAVGGKTARACDGCVKKRARWYCAADDAFLCQACDGSVHAANPLAQRHERVRLKTASMTQLSLEIPSWHYGFTRKPRTPRGKHHHRHKSEKLTTNPVHYEPEIVPNNENLSEENDEQEQLIYRVPIFDPFAAEMSLSDTSTESVTLRAGDDDGEKHKVGFYQSDMDLEEFAADVENLLCKGLDDQESFDMETLGFFNSRGKDSVESPSTWCAGTVIKFEEADEIKDSAIVNYQIEAEIDMTKETFELNFDDYDTPENNFEEDYEKAGGFGNVIDQLKEECEAMETNVEEKKSKILLALDYEGVLAAWPEQKSAWMTGDRPQLEDSTCWPDCMGECGNVHDNYASRYGEMGGLRMLDGGREARVSRYREKRRTRLFSKKIRYEVRKLNAEKRPRIKGRFVKRANFLAAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        </location>
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        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="314" mass="35863" checksum="A34158307DFC87E3" modified="2016-07-06" version="1">MMSSGTNAFALLGDQQGKEATSIIALKTAEIKKIADKNKKISAEKKKAEAKIKNKKKKAKAKIEEGQANEEQKTVDEHSGIQNQAKVSVRTAREEEEEEEKEEEARQSLAREKEKKEEEARRRECWLARQAELAREREERRLAWEAQLARQAEEQRLAREAELARQAESKARSAAREAEREERRLAREEERKKEIQERRNKEMVMKTLREYEEEKALENQKANKVILNLKEILKKDSHIKKHGKVRKAMNIEEFLKLPTGGGGGEGSEGQDGHNKESASSDENNSIKVKAGHAPDFSDESFPLLGKVSKPPKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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    <name>A0A164T563_DAUCS</name>
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        <recommendedName>
            <fullName evidence="9">Fungal lipase-type domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0727973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:phospholipase A1 activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase A1-II 3"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403">
        <property type="entry name" value="PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31403:SF53">
        <property type="entry name" value="PHOSPHOLIPASE A1-IGAMMA2, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="9">
        <location>
            <begin position="197"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH08532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="469" mass="53129" checksum="FBB416021E13F102" modified="2016-07-06" version="1">MAVPLSDVGWFFGSHSKEKNVCQSQNSVAFGGGSFAVSSRVRRCNMSTMVKSSQSENNMTVVIGEKAKSLAETWKEIHGENDWEAMLDPIEPLMKSELIRYGDMAQACYDAFDQDPGSKYCGGCQFKPDMFFEDLGLSKFGYDVTAYLYSAYNINFPIFYSKSLWPNSWSSSANWSGYVAVSNDETSASLGRRDITIAWRGTVVKREWLADMMDFLRPVTQREIDSRDPSIRVEAGFLQLYTDKDERCKFSEYSAREQIIAELSRLIQKYENEEMSITITGHSLGSALAILSAYDIAEAGIDLTKDGRDIPLCVFSFSGPRVGNARFKERLEWLGVKVLRVVNIHDKVPYMPGIFFNEHVSPIMRKVGELLPWCYSHVGEELALDHENSPFLKKTNDPRCCHNLEALLHLVDGYHGKGKKFWRSTGRNIALVNKSADFLKDYLSIPPNWWGTQNIEPNRNNMENVTGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TDC3</accession>
    <name>A0A164TDC3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_024518</name>
        <name evidence="3" type="ORF">DCAR_0727760</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TDC3"/>
    <dbReference type="STRING" id="79200.A0A164TDC3"/>
    <dbReference type="Gramene" id="KZM87384">
        <property type="protein sequence ID" value="KZM87384"/>
        <property type="gene ID" value="DCAR_024518"/>
    </dbReference>
    <dbReference type="OMA" id="PREFCKA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM87384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH08322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="98" mass="10682" checksum="6858F674857513DC" modified="2016-07-06" version="1">MPLLGVGTAHFPILPVLEVVEMNPVWQQVILREFCKAKGIMISIYSPLAAGGAIRGTRKVLDSEVLKEIAESKGKSVAQVALRWAHEQGVVIITKSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TMB1</accession>
    <name>A0A164TMB1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_024819</name>
        <name evidence="4" type="ORF">DCAR_0728501</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small hydrophilic plant seed protein family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM87718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164TMB1"/>
    <dbReference type="SMR" id="A0A164TMB1"/>
    <dbReference type="STRING" id="79200.A0A164TMB1"/>
    <dbReference type="Gramene" id="KZM87718">
        <property type="protein sequence ID" value="KZM87718"/>
        <property type="gene ID" value="DCAR_024819"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195720"/>
    <dbReference type="OMA" id="INRRTSW"/>
    <dbReference type="OrthoDB" id="540492at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038956">
        <property type="entry name" value="LEA_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022377">
        <property type="entry name" value="Sm_Hydphi_plant_seed_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000389">
        <property type="entry name" value="Small_hydrophilic_seed_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671">
        <property type="entry name" value="EM-LIKE PROTEIN GEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671:SF19">
        <property type="entry name" value="EMBRYONIC ABUNDANT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00477">
        <property type="entry name" value="LEA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00431">
        <property type="entry name" value="SMALL_HYDR_PLANT_SEED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM87718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="92" mass="9917" checksum="13E09FA58F5F4FFA" modified="2016-07-06" version="1">MASQQEKKELDARARQGETVVPGGTGGKSLEAQQHLAEGRSKGGQTRKEQLGGEGYHEMGRKGGLSNNDMSGGERAEQEGIDIDESKFRTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164U9X3</accession>
    <name>A0A164U9X3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_025689</name>
        <name evidence="3" type="ORF">DCAR_0729519</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM88614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164U9X3"/>
    <dbReference type="STRING" id="79200.A0A164U9X3"/>
    <dbReference type="Gramene" id="KZM88614">
        <property type="protein sequence ID" value="KZM88614"/>
        <property type="gene ID" value="DCAR_025689"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd04480">
        <property type="entry name" value="RPA1_DBD_A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM88614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="239" mass="26795" checksum="CD2F57D3E8CDC5D4" modified="2016-07-06" version="1">MAPQSALISSLSLNTKDVLLHVRATRIWESFSVDKKSSQRKMLNTKVVFIDEEQSQIMLTVWNNQKQDYFPLLKEGGVYDISQFRVVPNLTGYRIVNSEIALSFDHNTKVIPKEETERIPLFKFELTKFEDVPSLLWNTKNLIEVAGLVTEYGDPETASNGAKKMDILLLDSSNKDMIVTLWEEKANGFQNDLAAADDGAAFVIITGLLVKKYSGCLISICAILSPGVFKQSKLQTTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164UT07</accession>
    <name>A0A164UT07_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0730304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164UT07"/>
    <dbReference type="Gramene" id="KZM89321">
        <property type="protein sequence ID" value="KZM89321"/>
        <property type="gene ID" value="DCAR_026396"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOH10831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="284" mass="32477" checksum="FA9DC6AD2EB50E90" modified="2016-07-06" version="1">MGAWSLYSFIPLYSMNSSGLIFEKFLKFLTIKDIDSDEIQVPKKILGRYGDRLPERFFFNFRNGYELPVKFTKDTGIIKGMQTLYQDFDLTAGEMLLFEYNGMTDLNVYVIGKDKREIDYPHLVHSTQKRVPRVVSMKNGGIRFINFVTDQDPLADEFEPPLSFEISCPLVPAYQSFVFSNGKEFEARYDAQRRRFRGLSAFCRDVGIDDFSGFNLLLFQYELYGNVTISAFDDAFVEVMFVGGPLSVGSISTNPSIHGSFSITVQPFHMSKYCYGVVLIFPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X7A7</accession>
    <name>A0A164X7A7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0626670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X7A7"/>
    <dbReference type="Gramene" id="KZM92797">
        <property type="protein sequence ID" value="KZM92797"/>
        <property type="gene ID" value="DCAR_019838"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225572"/>
    <dbReference type="OMA" id="THINMAY"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000479">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000062">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF56">
        <property type="entry name" value="LOW QUALITY PROTEIN: PROTEIN STRUBBELIG-RECEPTOR FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043926762" evidence="12">
        <location>
            <begin position="25"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH07241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="732" mass="80604" checksum="BA04A4935EB35842" modified="2016-07-06" version="1">MSARSLLLYFTLLVFFALGLQASAETDVLDSKALQDLYRTLNIPSQLKGWKTDGGDPCAESWTGVTCNGSSVIHLIIPGLQLGGNLGFQLSDLRNLKQLDISSNYIQGEIPYNLPANVTHLNLADNNFSQNMPYSLTNMKHLRHLNLSHNSLSGPVGNVFTGLTNLKEMDLSYNNFTGDLPSSFGDLKNLTRLFLESNGFTGSVIFLSNLPLSDLDIQDNHFSGVIPETFENIENLWIGGNRFHRGENYPPWFFPLDTTPNITSPPSTESSAVESHPSHHEHKHKKKGFGPGGIASIVCGVTLLAACAAFFAVARIQQRGRSLRRLESSPGSWQSLPVSTAREFSSTAPEESSELSAISSPPLIDLKYLPPLRARTVRTSRRSFSRKSRVPVGTKIYSVAELQAATDSFSEANLLGEGSLGSVYKAEFPGGQIFAVKNINTVALALHEEEKFLDVIWNVARLRHPNIVKLVGYCVEHKQHILVYEHVRNLSLDDALHCDSYMPLSWGLRLRVALGVARALNYLHSTCMPPIAHSNLKAANILLDEDLMPHICDTGLAVLKPLTSNKIKIKASEMAIGDTGYIAPEHIQSGIGNMKGDVYAFGVLLLELFTGRRPFDSSKPRGEQSLVSWASSQLHDNESLEEMVDPAIRRTIPAKALSRFADIISLCIQAQKEFRPPMTEIQEGLMCILQEHRVVRGHTAAADGTEADPLDRSFRSSNSRFFASPTLSYLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YBJ5</accession>
    <name>A0A164YBJ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0520031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH00658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164YBJ5"/>
    <dbReference type="Gramene" id="KZM94261">
        <property type="protein sequence ID" value="KZM94261"/>
        <property type="gene ID" value="DCAR_017504"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224078"/>
    <dbReference type="OMA" id="YSLAKGY"/>
    <dbReference type="OrthoDB" id="1690600at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOH00658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="166" mass="16716" checksum="3E244B26F0ADA0F5" modified="2016-07-06" version="1">MSSTAFSGDETAPFFGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGIYGLIIAVIISTGINPKAKSYYLFDGYAHLSSGLACGLAGLSAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVGIILSSRAGQSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164YWW5</accession>
    <name>A0A164YWW5_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0520894</name>
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    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH01510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="127" mass="13977" checksum="C73E5F5310A9E96A" modified="2016-07-06" version="1">MSLASEKVVLCELGGRKEIVRCFEDEDFKSSNADGTSFYNIVLEYADGGTLQQLIKSKGWILNMKLVADFGLAKKGGEKSLGAGEEYKNRGTLLYSSRNPLFGVHEAAMDIWAVNCIVLELLLGEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZS27</accession>
    <name>A0A164ZS27_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_019560</name>
        <name evidence="4" type="ORF">DCAR_0522378</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZS27"/>
    <dbReference type="STRING" id="79200.A0A164ZS27"/>
    <dbReference type="Gramene" id="KZM96318">
        <property type="protein sequence ID" value="KZM96318"/>
        <property type="gene ID" value="DCAR_019560"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219996"/>
    <dbReference type="OMA" id="AWGGYPQ"/>
    <dbReference type="OrthoDB" id="4150at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000037">
        <property type="entry name" value="BnaA05g14690D protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017506">
        <property type="entry name" value="PSII_S4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040591">
        <property type="entry name" value="RqcP2_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03069">
        <property type="entry name" value="PS_II_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633:SF3">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17774">
        <property type="entry name" value="YlmH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="2">
        <location>
            <begin position="238"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM96318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH02988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="33803" checksum="2363F0AC0437BFFE" modified="2016-07-06" version="1">MAATTAVRSFLNSTARSVKQHNLSHLGTRPFPRLVFKSPLTSSGMCHVAQATRGNTDVLVRVVGEKGAVDEVKHVVEMAKRASLRREVLHTDFLTPPVLKESMQVLEKLADVKAVAQGGYPQAERCRLSVGHPEALTSDPDIVEALSISGNFGFEPCSHGDFLGSILGTGISRDKLGDILLQGEKGAQILLVPDLVDFLTSALNKVGNVTVTCKRIPLLALEYEPPRTKSFKTIEGSLRLDAIASAGYKISRSKLVDLISNGDVRVNWASVSKNNTTIKTGDMISVSGKGRLKIGEINSTKKGKFAVELIRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165ZYQ8</accession>
    <name>A0A165ZYQ8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0310539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165ZYQ8"/>
    <dbReference type="Gramene" id="KZN00564">
        <property type="protein sequence ID" value="KZN00564"/>
        <property type="gene ID" value="DCAR_009318"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108213516"/>
    <dbReference type="OMA" id="EAHAYSW"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000019">
        <property type="entry name" value="Clathrin coat assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF62">
        <property type="entry name" value="ASSEMBLY PLANT-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG91291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="605" mass="66696" checksum="9E8F6D41AAD31F0F" modified="2016-07-06" version="1">MAPTTIRKAIGAVKDQTSIGIAKVVGNVAPDLEVLIVKATSHDDEPADDKYIREILNLTSHSKGYVDACVFAISKRLSKTHDWIVAIKALMLVQKLLVDGTPLFGEEMMHASRKGTRVLNMSGFRDEAHAYSWDHAGFVKSYALYLDQKIEFSVYDRRMSGLDDKYSEFRVEYGHGGNNRPRAYGDLDEPVVRGVREKGTPLREMKTEKVSQRLNQLLRLLDRFLACRPNGAAKDSKIILVAVYSLVKESFKLYADLCEVLHILLDRYSDMEYEGCLKSFDTYVGAAKMIDELVGFYSWCKDTGIARSTEYPELQEISDKLMEKLEGFLRERAIRGNSREKPMEVNTSVIQKEKLPDFSEIKALPPLKDYDLSMPQAQPKPQAQQITEDLVNIKDNAMSVEVQGNKLALALFSEAPPINADGSWEVFPSDGGPDMTSAWQTPAAEMGKADWELTLVETASNLSKQKADMGGGIDSLLLSGMYDQGAVKQHNSNAQLNVGSASSVTLPGTGKGSTPALALPAPDGTVQTVGNQDPFAASLVVPPPSYVQMADMEMKQHLLVQEQRLWQQYANNGMQGQVGSANVTGGTHGCYITGQPGGYNYHSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A711</accession>
    <name>A0A166A711_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0310808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A711"/>
    <dbReference type="Gramene" id="KZN00814">
        <property type="protein sequence ID" value="KZN00814"/>
        <property type="gene ID" value="DCAR_009568"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214079"/>
    <dbReference type="OMA" id="RIRRNHA"/>
    <dbReference type="OrthoDB" id="1875050at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038934">
        <property type="entry name" value="At5g64816-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35548">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35548:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG91559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="128" mass="14215" checksum="5B1F558A2FE705ED" modified="2016-07-06" version="1">MVDWWWSVLGAAVPAVVGGQAWRMKNRRAEEQRLKSVRGREKSSDEIFVCERVCTSKRMLKKVGAFSKEPTPDTCVTVCGVSELDACADACARTVCVNQHQVPNWNDTCLKRCQSECLRLSSSASTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166BUS4</accession>
    <name>A0A166BUS4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannitol dehydrogenase</fullName>
            <ecNumber evidence="9">1.1.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NAD-dependent mannitol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_011657</name>
        <name evidence="15" type="ORF">DCAR_0313165</name>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DLU6"/>
    <dbReference type="Gramene" id="KZN05408">
        <property type="protein sequence ID" value="KZN05408"/>
        <property type="gene ID" value="DCAR_006245"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208002"/>
    <dbReference type="OMA" id="KQPEWFI"/>
    <dbReference type="OrthoDB" id="1931827at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034565">
        <property type="entry name" value="Put_cell_wall"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733:SF1">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007872266" description="Cell wall protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN05408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13508" checksum="8BF9CFA6753272C8" modified="2016-07-06" version="1">MAMKATSVLALLVIFNVLVSSVMGRDIPKDSKTAEVKKPETFNQEGSVLIPGVGRVMIPPKKKCFFKGFNPFTYNPVTGKNTGHGISIPTLPNTGVPGYLPGNDDTFIPNPGFEVPTGFPAPPIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DN07</accession>
    <name>A0A166DN07_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_006291</name>
        <name evidence="9" type="ORF">DCAR_0207099</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN05454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DN07"/>
    <dbReference type="STRING" id="79200.A0A166DN07"/>
    <dbReference type="Gramene" id="KZN05454">
        <property type="protein sequence ID" value="KZN05454"/>
        <property type="gene ID" value="DCAR_006291"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205871"/>
    <dbReference type="OMA" id="MDWVREQ"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13136">
        <property type="entry name" value="MATE_DinF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044644">
        <property type="entry name" value="DinF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893:SF45">
        <property type="entry name" value="PROTEIN DETOXIFICATION 45, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN05454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG87867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="608" mass="65243" checksum="35C9477364B9F997" modified="2016-07-06" version="1">MAASQVSGGGTSSRLFTRELNPTTRMRRSLLLQNSSQSRNFNMLVNGKRLRCIDIVARASLSTNYTQMSSPLPIMRQRRRPLSDVNYKLSSDSSVQTSDMDESLSIEEEFEPSTSEDTSHDLNENSSSVASDKVKAELKGNDTNPTHELITLSLPAILGQAIDPFAQLMETAFIGRLGSVELASAGVSISIFNIVSKLFNIPLLSIATSFVAEDISRNAHSRSNSEGHEEINNARPLEAMAKRQQLSSVSTALTLAIGIGLFEAVALWLGSGIFLNLMGLSSASSMHAPAQRFLSLRAIGAPAVVVSLALQGIFRGFKDTKTPVICLGIGSAATILLLPVLMYFLKLGVTGAAISTVVSQYIVTCLMLWHLNKRAVLLPLKLGSLQFRGYLKSGGFLLGRTLAVLITTTTGTSMATRQGPLAMAAHQICLQVWLAVSLLTDALAASGQALISSSASKGDYRSVKEVTYSVLKIGFGTGILLTLILGASFSSLATLFTKDAEVLAIVRTGVLFVSASQPINALAFIFDGLHYGVSDFKYAACSMMVVGAISSIFLYYAPSVFGLPGVWAGLTLFMGLRTIAGVIRLASKDGPWWFLHKDSERLKFSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166G0R9</accession>
    <name>A0A166G0R9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_000961</name>
        <name evidence="8" type="ORF">DCAR_0100917</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN08415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166G0R9"/>
    <dbReference type="STRING" id="79200.A0A166G0R9"/>
    <dbReference type="Gramene" id="KZN08415">
        <property type="protein sequence ID" value="KZN08415"/>
        <property type="gene ID" value="DCAR_000961"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108208078"/>
    <dbReference type="OMA" id="AKPYHPL"/>
    <dbReference type="OrthoDB" id="1924702at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006904">
        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47830">
        <property type="entry name" value="OS11G0534100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47830:SF1">
        <property type="entry name" value="OS11G0534100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZN08415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG81766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="311" mass="34242" checksum="301753CF74D49351" modified="2016-07-06" version="1">MAETAVLYQTFITTGLISMGTYHLISTVKNYLKSPSHYSAKPYHPFTSTSSYKKYIQLYLSLVFLFFSFLHQTLISSHTDLLLKGNTPVHKFISLQYAAVSVLFILLCLAILLSETTLLLPFSNDLFFALASGVFFFQYYVSSSAAQFQISDLQAHCDSVSAKISAVSSLLSFLLACFPKLFVADVLLCGSLILQGFWAMQTGLTLYVDAFVPQGCHKLLDVVTGVEGSTRCDLDDSKFRAVAILDLMFLVHVFFVMIIVLLVYALVAKMLGVRSSRFGSYEALPTVIPASASSITDSNHIQMKALTGTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GMF2</accession>
    <name>A0A166GMF2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0101772</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GMF2"/>
    <dbReference type="Gramene" id="KZN09128">
        <property type="protein sequence ID" value="KZN09128"/>
        <property type="gene ID" value="DCAR_001784"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205444"/>
    <dbReference type="OMA" id="NIAIKIR"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000010">
        <property type="entry name" value="Probable protein phosphatase 2C 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="31"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG82607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="280" mass="30664" checksum="5252EAFAA52964FC" modified="2016-07-06" version="1">MDKLCCFSSQASEGHSTHGTGKKRGGRGTIKFGFHLVKGKASHPMEDYHVAKFVRHQGHVLGLFAIYDGHLGDGVPAYLQKHLFNNILKEKEFWTDPNRSISRAYERTDQAILTHNPELGKGGSTAVTAILIDGQSLWVANVGDSRAVLSRRGQAIQMSIDHEPNTERGSIENKGGFVSNMPGDVARVNGQLAVSRAFGDKNLKNHLRSDPDVKDAIVDEDADLLILASDGLWKVMSNQEAVDIALKIKNPQKAAKQLSVEALRRDSKDDISCIVVQFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H5C2</accession>
    <name>A0A166H5C2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uncharacterized GPI-anchored protein At5g19230-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0102493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H5C2"/>
    <dbReference type="Gramene" id="KZM80048">
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        <property type="gene ID" value="DCAR_000459"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN09744">
        <property type="protein sequence ID" value="KZN09744"/>
        <property type="gene ID" value="DCAR_002400"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192555"/>
    <dbReference type="OMA" id="SSENNWI"/>
    <dbReference type="OrthoDB" id="753138at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045285">
        <property type="entry name" value="At5g19230-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059083">
        <property type="entry name" value="At5g19230_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33976">
        <property type="entry name" value="OS07G0645000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33976:SF8">
        <property type="entry name" value="OS07G0645000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25884">
        <property type="entry name" value="At5g19230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uncharacterized GPI-anchored protein At5g19230-like" evidence="1">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG83318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="199" mass="21743" checksum="A070907B7697A91E" modified="2016-07-06" version="1">MVWFQHYLLLSLVIFSYLSIHHTAKCDDEEESLLLGINSYRATLKLPTLTDNDKAECLAEEIAEQFKNTPCSNTTGANTVPGTEPQFSDFPALLNKCKLNVTTTRDGTIMPACVPNLVQDVVLSNYTQSQYAAFLNDTKYTGIGIGSDEDWIVVILTTGTPTGNFATGSSVPDNFAAKLSPIQPMLCIVIGIFYLIGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HXM0</accession>
    <name>A0A166HXM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_003171</name>
        <name evidence="6" type="ORF">DCAR_0103353</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HXM0"/>
    <dbReference type="STRING" id="79200.A0A166HXM0"/>
    <dbReference type="Gramene" id="KZN10515">
        <property type="protein sequence ID" value="KZN10515"/>
        <property type="gene ID" value="DCAR_003171"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195944"/>
    <dbReference type="OMA" id="EAENQAW"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647:SF22">
        <property type="entry name" value="BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647">
        <property type="entry name" value="SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036836">
        <property type="entry name" value="RNase_bind_SBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KZN10515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG84172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="32540" checksum="56585373A5823ABB" modified="2016-07-06" version="1">MAIQSQFYSENFGFNADNREVVCQDNMYAEHGFGFESELNVNFHQQQKQPFMEFQPQQQHQFQFMPRRFESLCSEKNVFTPNYANQSLNLSSTISAVMEKQRQEIDQFLSVQNERLRLALLEHRRQQVAVILKRCESKAMVLLKQKDEEIARALNRKLELENVIKAMEIESQKWHRAAKENEAMVMSLNNTIEQLKENAICLSLNNNGGADQDAESCCDDNRGVGFQEDEEQRRICRSCYTCNSCVVFIPCRHLASCKACDAFLDTCPVCGMAKKSTIEALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IHZ5</accession>
    <name>A0A166IHZ5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_003805</name>
        <name evidence="9" type="ORF">DCAR_0104039</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IHZ5"/>
    <dbReference type="Gramene" id="KZN11149">
        <property type="protein sequence ID" value="KZN11149"/>
        <property type="gene ID" value="DCAR_003805"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204974"/>
    <dbReference type="OMA" id="RPMYSAD"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241:SF12">
        <property type="entry name" value="OS03G0784100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="65"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZN11149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG84854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="425" mass="45348" checksum="838254B44B3F73EE" modified="2016-07-06" version="1">MAAALSMLESNYEADKLTYEIFSILENKFLFENKNPQFCLPKQTALPLEKLKSGKHITGKVRILSIDGGGATNGLLAAKSLVHLESNLQRKSNNPNARIAHYFDVVAGSGAGGVLAALLFTAGKDGRPMYSAQDALNFMVENHGKISRSSPEGIFRRVFRSSESVLGKTFGDLSLKDTMKAFLVPCYDLNSCGPFVFSRADALEMDGYDFSIKDMCAATSAVQSVRVKSVDRKTKIAAVGGEVAMNNPTAAAITHVLNNKQEFPFCNGVDDLLVVSLGNGESFSGGVAGNATPLRSAVVRIAGDGVADMVDQAVSMAFADCGTSDYVRIQANGTVGASQGSLKGDKKGLLSRVEEMLRMKSIESVLFKGKKLADGSNLDKLELFSTKLIKEEERRKTSILPAVVLKQAGSPRTSSATTLSTTSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IRS6</accession>
    <name>A0A166IRS6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_004073</name>
        <name evidence="2" type="ORF">DCAR_0104347</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IRS6"/>
    <dbReference type="STRING" id="79200.A0A166IRS6"/>
    <dbReference type="Gramene" id="KZN11417">
        <property type="protein sequence ID" value="KZN11417"/>
        <property type="gene ID" value="DCAR_004073"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108196471"/>
    <dbReference type="OMA" id="CCTAVEV"/>
    <dbReference type="OrthoDB" id="691424at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF2">
        <property type="entry name" value="DUF506 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN11417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="304" mass="34552" checksum="9F59091D72D00FF9" modified="2016-07-06" version="1">MAGVMTRIPMSHRIFTPYNSRDIAFPGELALSMSDTVFGFLHEESEGSPESILYEEYNRNDQEEDEEAENSTSNAEDHKKFWENQYQLLQATLYRTSSLESKIRNCTKEVLREAQAAGNECTCRNPVFGGCRICLMKQVSASLQNAGFNAAICNSKWKNLPDMPSGEHTFVDILDSNTKKGEVRVIVELNFRGEFEMARASEEYNRLVSKLPEVFVGKIERLLSITAILCAAAKKCMKEKKMHLGPWRKTRYMQAKWLRVTERQTAVPRLISTTAGYSIRPQRPRASMLTVDLLEKLPSVQCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J7W9</accession>
    <name>A0A166J7W9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_004543</name>
        <name evidence="17" type="ORF">DCAR_0104919</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in plant defense signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J7W9"/>
    <dbReference type="STRING" id="79200.A0A166J7W9"/>
    <dbReference type="Gramene" id="KZN11887">
        <property type="protein sequence ID" value="KZN11887"/>
        <property type="gene ID" value="DCAR_004543"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212571"/>
    <dbReference type="OMA" id="KPFTANC"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="89"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KZN11887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG85727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="412" mass="46314" checksum="0A2B441057E2C09B" modified="2016-07-06" version="1">MKESKPWKPFTANCCSAEDHTIFRNFSRCRTSRSDFSKNVAPMPSFRLISVSDISRTSSSRINEDLMQTFGQDLFDFQLTELRAITQNFSSSFLLGEGGFGTVHKGYVDENMRAGLKAQAVAVKLLDIEGLQGHREWLAEVIFLGQLRHPNLVKLIGYCCEDEERLLVYEFMPRGSLENHLFKRISVSLPWGTRLKISIGAAKGLAFLHGAEKPVIYRDFKTSNILLDSEFNAKLSDFGLATMGPEGSETHVTTRVMGTYGYAAPEYVSTGHLTTKSDIYSFGVVLLELLTGRRAMDKTRAKTEQNLVEWSKPYLTSSRRLRYIIDPRLLGQYSVKGAKEMANLALHCVSLNPKDRPKMPQIIETLEAVQPLRDMAVTCGHWPVSPKTGRNVSNGNNSMKKKESPVFVNRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WGB6</accession>
    <name>A0A169WGB6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0314296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Interacts with RANBPM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WGB6"/>
    <dbReference type="Gramene" id="KZN03858">
        <property type="protein sequence ID" value="KZN03858"/>
        <property type="gene ID" value="DCAR_012614"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215371"/>
    <dbReference type="OMA" id="YEDHCCS"/>
    <dbReference type="OrthoDB" id="972532at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000087">
        <property type="entry name" value="WD repeat-containing protein 26 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF23">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN WDS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23627">
        <property type="entry name" value="LisH_WDR26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG94994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="521" mass="58685" checksum="3B5A78ACFA86738E" modified="2016-07-06" version="1">MENSSTTLDGKGLIKKNEFIRLIIQCLYSLGYEESAACLESEANVSYKSAELKLLESQVLGADWDDCVDTLYGIKDLTDEVRASAVFLVFKQCLLEILSRGDDTMALALLRKQILSSNMGREKVHKLAFGMLARKEMVKVEDHIICELRKRLLTKLEIVLPPPIALPERRLEHLVEMAVSAQIDSCMYHSAFASVTICEDHHCGRDQIPTETYQILTDHTNEVWFVQFSNNGQYLASSSSDCSAILWEVMDDGELRLKHTLQSHQNPVSFVAWSPDDTMLLTCGNAEVLKMWDVETGTCKHTFGDQGFMVSSCAWFPDSKRLVCGSCDPEKGIYMWDTDGNEIKAWRGMRMPKVLDLAVTPNGESLITIFSEKEIRVLNLVTNDERVISEEHPITSLSVSGDSKFLLVNLNSQEIHMWDIAGTWAKPLKYKGHMQHKYVIRSCFGGFNSNFIASGSEDSNVYIWNRRSSDPLEILVGHSMTVNCVSWNPKRPNLLASASDDQTIRIWGPCSSDSRKLLESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WIB9</accession>
    <name>A0A169WIB9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_012665</name>
        <name evidence="3" type="ORF">DCAR_0314353</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG95051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WIB9"/>
    <dbReference type="STRING" id="79200.A0A169WIB9"/>
    <dbReference type="Gramene" id="KZN03909">
        <property type="protein sequence ID" value="KZN03909"/>
        <property type="gene ID" value="DCAR_012665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN03909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG95051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="25892" checksum="39DA466EEE245762" modified="2016-07-06" version="1">MEYERIHKLQVYSDTAEVTFCLRCYQPTDERLDSFLSFLKDKGLKLDEVEIRKANEDAENKGDGKSSGDDSVEDTTSESLQPDNIPNMENTILVALALEKAALSDEYFVKWKLYPNVDLYSGCIEFFPVLFAIPRMAGYLSHWKESLNDPCTTIMRHAQVYTGVWLRPYIPQRERMVATETDKLSQISVSNATRRRLAANSLCRFWLFSTRDVSTYYPIYEDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WN35</accession>
    <name>A0A169WN35_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_005707</name>
        <name evidence="15" type="ORF">DCAR_0206416</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN04870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WN35"/>
    <dbReference type="Gramene" id="KZN04870">
        <property type="protein sequence ID" value="KZN04870"/>
        <property type="gene ID" value="DCAR_005707"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108205976"/>
    <dbReference type="OMA" id="MHNDVCY"/>
    <dbReference type="OrthoDB" id="1889525at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF164">
        <property type="entry name" value="OS05G0485000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="51"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="12">
        <location>
            <begin position="489"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="13">
        <location>
            <begin position="628"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KZN04870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG87193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="792" mass="88460" checksum="27E2D7CA0E1933E1" modified="2016-07-06" version="1">MDSPASNNASRVCSIMDHSNSKGLWFGDNPLMFYVPSLLLHLSLVNILTKCIHFFLKPLGQPTIISQTLAGVVLGPSILGQSTTFLSNVFPKETRIVLETTAAFGFMLFIFLIGVKVDPMMVYRTGKQPLIIGILGFFIPYGLASFVAFLLQRYAYLDRDTFDGIPFIVAVMSMTAFPVVTCFLDDLKILNSEIGRLASSSSIICDVCNWAVMVTNYTVRLADKTRSFRITIGSVFSTLLYLFTIVYGIRPAALWAVRRTPEGRPVKELYIFLVLVTLMICGFIGELIGITAFGASLALGLVIPDGPPLGAALTEKLDSFVEVFMPLFFVVSGLQTDVFAIKQMDNVGAIQLLACASFVGKVLGTILPPLCCRMPIRDALSLALIMNTKGIAELGFMIQMKHMNQLTAEPYTIMVISVVVITGVISPIVKFLYDPSRRYLAYRRRTILHLRRNEELRVLTCLHSPENVQAVISLLQASNPTKESPINLVVLHLVKLIGRASSLLVPYRQREKPSSKRSESEQIFSAFRKYEQLNHGSVFVNNTFKGISPYATMHDDVCSLALEKRSILIILPFHKQWIYGETVETSHAFRNLNKKVLDKAPCSVGVLLDRVKQKNPRYVLSEQLLQKVAVLFFGGPDDREALSYGQRMSRNSTIELHLVRFITSTSQNIVGGKERSKMLDDNILSDYKHNTMSSKRVSYQEEVVSSGKDVVSSTRSVGVSHDLVLVGRRHGESLLMYQLKKWRDKGELGEVGEILAYPEYNCEASVLVMQQQTKLWGLQDPEESTHLRKCDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WUY0</accession>
    <name>A0A169WUY0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102675</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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                <person name="Macko-Podgorni A."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KZM83148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YJ24"/>
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        <property type="protein sequence ID" value="KZM83148"/>
        <property type="gene ID" value="DCAR_030717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008044617" evidence="1">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM83148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH16142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="96" mass="10197" checksum="26ED6151D429172F" modified="2016-09-07" version="1">MGKTIFGILCVCVVYATIGINLPVTIGEVNNEARGEGPWYHNLGGHPIFSNTAIPVNNCNQQSCRSACLSSKPGGNLASVNCRDDKTCVCVWLNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YL77</accession>
    <name>A0A175YL77_DAUCS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_028230</name>
        <name evidence="3" type="ORF">DCAR_0831078</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YL77"/>
    <dbReference type="Gramene" id="KZM84223">
        <property type="protein sequence ID" value="KZM84223"/>
        <property type="gene ID" value="DCAR_028230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM84223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH11588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="583" mass="66931" checksum="C9921884753EC987" modified="2016-09-07" version="1">MAHFIYMEQSHALAHYERPEIPNIYSEIDEETVIHSEDMSVSDLKRQEQALDYYNRTKESLPQDPAGISRIESQIQERDFQANAPYQEDDGFQYLFDTSQIEEQDDWHTLKLLVYLERKKGSISPTQHVNQGRFLTRRSQLEAKHNRLVRLYGKRPLNKHDFDSKLGYSMYRMSWFIWGDHVIQSDGSDDLDDYGEFFDPNAPFSHPDMKCNPYDPKENNVSDDSVVDVDMTGSYPDLKEVLKPDDPIFDEIDDLDLNEIFHWIDVCFGSQFDSTPASASGVSADKSHIFKGPKPQAVKDTDDADLTLATEMSLGKVSEDTKSKISEDQPAPEMDTLPRKRRMTDPMPEAIENKQSKRRKLLYSPPKHGVNQEEERCLLLNTDNEYDEDYEILWRLYWYTVNQRTSIFQGGAFVLDFENGVTKFINLFLDSKITTDQCKKKVMELREKFLCFMELNGNEDFNEADFYVPHEYKMFQMFKIVWGDDRGRGGSCSVADPISATVTNVSGAGKAPEPANVKSGAADKELEADMARAVSNSLKEIREDTMLQGAISDIRELDMKVDSGSVQDSGENIYVQDNMDIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YR17</accession>
    <name>A0A175YR17_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_026499</name>
        <name evidence="3" type="ORF">DCAR_0833164</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM86079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YR17"/>
    <dbReference type="Gramene" id="KZM86079">
        <property type="protein sequence ID" value="KZM86079"/>
        <property type="gene ID" value="DCAR_026499"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM86079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH13654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="275" mass="27252" checksum="CF89C3F0A6E23FF3" modified="2016-09-07" version="1">MASSATAVSKPTRKLVGDLDFFSAGGAEAPAADSLSSFGAAAPSSGLENKLFAILSHESTQMQSFLEHSDYSEELLEVHSSIPVGMASTASATKIEYRKFATGAAEFFTGTGGASAPSPGPSAAEAPSLGPSAAEAPSPDSEPSTQVLGTSVTEPSKEAGKPESEPSTEPGITDHADHPGHSKHSKHPKHSGSHSKSHPSSSIPSGGDQAATKVMSDSTGSLPEPQGPSSADAPSTTQASDTLGALGPSSTILGALGPSSADASSPGLAPAQPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W451</accession>
    <name>A0AAF0W451_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0101807</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W451"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
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    </dbReference>
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        <location>
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            <end position="360"/>
        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82641.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="452" mass="51299" checksum="A359ECC98ED9F1F4" modified="2024-05-29" version="1">MICNSIKQSKLRKHPNHESTTTVCQEIKANGLLIRWLNIFHQDRTLGYKVFGSTENYQPALNSIFLFDNLCSLFYQCKLRYKSYLQSGERCDQVVIANHLTGPISKMYATTCEKFSEDPPMADPVSDMLVDSFGRLHTYLRISLTERCNLRCQYCMPAEGVELTPSARILSQNEIVRLANLFVSSGVDKIRLTGGEPSIRKDIEELCQRLSNLEGLKTLAMTTNGITLSKKLPKLKEYGLNLVNISLDTLIPAKFEFMTRRRGHDRVIESINTAVDLGYSPVKVNCVVMRGFNEDEICDFVKLTQDKPINVRFIEFMPFDGNVWNVKKLVPYSEMLERVEKQFKIERIKDHHTETAKNFRIDGHQGTVSFITSMTEHFCAGCNRLRLLADGNFKVCLFGPSEVSLRDPLRSGVGDDKLREIIGAAVKRKKASHAGMFDLAKTPNRPMIHIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W5U2</accession>
    <name>A0AAF0W5U2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102973</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
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            <taxon>Pentapetalae</taxon>
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            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L4"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="296" mass="33366" checksum="CE51B7887162E708" modified="2024-05-29" version="1">MASSNGFKLLRLLSRQSLRPTFLHDSSPISVIFTLPLYFATGQPFLAFRKFSTSILTPESSEGTFPSDLLSRGQVLTPERLYQDLVIPVTNFNNEDKGYMVLAGDVFDVPIRKDIIHRVVRWQLAKRQQGTHSTKTISEVSGTGRKPWPQKGTGQARHGSRRGPQWRHGATMHGPKPRSHEIKLNKKVRRLGLKIALTARAAEGKLAIFDDLDVPTHKTKNIVNYLKHMENTKKLLLVDGGLINEKLKLSTQNLHYVNVLPSIGLNVYSILLHDTLVMSRDAVNTIVERMHTPINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W6W8</accession>
    <name>A0AAF0W6W8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0103426</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W6W8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000036">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000026">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000006">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000011">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000011">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024750">
        <property type="entry name" value="Ca_ATPase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF448">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12515">
        <property type="entry name" value="CaATP_NAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="395"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="920"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="959"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="116"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG84244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1037" mass="114035" checksum="115C9A62F0086BDB" modified="2024-05-29" version="1">MEYYLKKNFEVEAKNTSEEALKKWRDAVWLVKNPRRRFRMVADLDKRAEADRKRQDIQEKIRIALYVQKAALQFIDAVRIKSDYKLSDKVRDAGFGIEPEELQSINRSRDAKCLDAHGGVKGFALKLKVSLKEGVMSSNVPLIQDIYGYNRVTEKPMKPFWMFVWEALQDMTLIILMVCAVVSVGVGIATEGWPKGMYDGVGIILCIILVVVVTAVSDYKQSLQFKDLDREKRNIMIQVTRDGSRQSVSIYDIVVGDIVHLSIGDQVPADGLFISGYSITVDESSLSGESEPVNVNAKNPFLLSGTKVQDGSGKMLVTSVGMMTEWGRLMLTLSEGGDDETPLQVKLNGVATIIGKIGLAFAVMTFVVMTARFVIVKAVHHDISKWSMADALNLLNFFAIAVTILVVAVPEGLPLAVTLSLAFAMKKLMNDRALVRHLSACETMGSANCICTDKTGTLTTNHMEVTKIWICDEAKDIGSADNTGNVLESSISENALSTLLHSIFHNTNAEVVKDKDGKTNIMGSPTESALLEFALLFQGDYMAQSKKAQIMKVEPFNSIKKKMSVVVALPGGGHRAFCKGASEIILGMCDKIISKNGESVPLSEEQRKNITTVINGFASEALRTLCLAFQDMDTSKSNADSIPEDSYTFIAVVGIKDPVRPGVREAVKTCLRAGVKVRMVTGDNIHTAKAIARECGILTDDGVAIEGPNFRDKSAEELQDIIPKLQVMARSLPLDKHKLVQLLRNEYQEVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKENSDIVVMDDNFKTIVTVAKWGRSVYINIQKFVQFQLTVNVVALMTNFVSACVSGSAPLTAVQLLWVNMIMDTLGALALATEPPTDELMQRPPVGRNVHFITKIMWRNIIGQSIYQLIVLGILKFDGKRLFHLHGADSFSVLNTLIFNTFVFCQVFNEINSRDMEKINIFKGMFDSWVFMMVMVSTVAFQIVIVEFLGTFAETVPLSKELWLASVLMGAVSLPIGALLKLIPVSSIECPRHHDGYEQLPSGPDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7J2</accession>
    <name>A0AAF0W7J2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0103896</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7J2"/>
    <dbReference type="KEGG" id="dcr:108223432"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF43">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG84712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="314" mass="35456" checksum="2702C433806EAD80" modified="2024-05-29" version="1">MKLPPGYRFKPLEEELITLYLKPRVLAQQDPFNAVQECQLYGPNANPWHLLPPDTDSWLLSEVSPGKLEKVTYVFVSLTKKSGKMKNNPRAAKENYTKKAGCGTWDGQTKRYEIRERDSGDLVGERRFLVFEINDVEGVELSKVGHWKMHEFHLSGVNERIANPCNVVLCKITLDLSKGPLVKLKNVCDPPVKSGRKFQHRNANVDRNQGPVSCQRSRRDSEMLESGRVEEKEGDKGNNIVEMCDRVECMSIDHGNDCAGSVNSPSEQVNTENFEPAMIAQENKEEESGRGKFLFGMTKVQFSGHSNPQEEDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W932</accession>
    <name>A0AAF0W932_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0104758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W932"/>
    <dbReference type="KEGG" id="dcr:108205237"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF5">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="4">
        <location>
            <begin position="80"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG85567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="558" mass="63176" checksum="89C0E5C01CF83603" modified="2024-05-29" version="1">MASDTQGSVELKQEPPQRIQIYPTPSNEVPPFWRLKYEREAKKYWDNFYKRHQDRFFKDRHYLDKEWGQYFSGAGKKVVLEIGCGAGNTVFPLVATYPDIYVHACDFSPRAVELVKTHKDFIETRVNAFVCDLTAEDLTKQISPASVDIVTMIFVLSAVSPEKMSQVLQNIKMVLKPNGFVLFRDYAAGDLAQERLASKDQKISENFYVRGDGTRAFYFSEEFLRKLVKQIGFDTKEHSIHCKQVENRSREIVMNRRWVQAVFQLNSLSYSSITETEIKLQLLSKESSKSDNRVIESATDSEIDVSEGIIEMFGMSNSRNEIIEVNVRDWSFKIKVLPKENQHTCKSTGLMLWESAHLMAHVLATNQHIVAGKKVLELGCGCGGICSMISTESADLVVATDGDEKALELMSENVSSNLQSSSLSKLIVKRLEWGNKDHIEEIKDLKSGGFDVIIGTDVTYVAEAILPLFATAKELISSNKNINQESEPAFILCHIFRRVDEPSILSAASKFGFRLIARWPDESVTASSHSIISDWFKEDTEDECLPSKALNIMYFTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WAU8</accession>
    <name>A0AAF0WAU8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0205280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WAU8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF590">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="173"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG86082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="311" mass="36759" checksum="BE1E167822CDEE4C" modified="2024-05-29" version="1">MINPESGPYILAFNQTRPKNNPIIFRANFRAISVVLLVYIACKGTGFFYLINHGIEIELIQKALEESRNFFCLPLEEKMKLAWKEHRGYTPLCSERLDASLRYQGDLKETIYIGPIEDDKSYLNQWPSREILPSWRFTMELYYEKVRAAGRRLISLIALALNLEEDFFEKDGPVDPFLRLLHYPGVLDEKVLGSSAHSDYGMMTLLVTDGVPGLQVCREKAKKPQVWEDVPHISGAFVVNIGDMMERWTNCLYRYYLLERWNNFKYLNEIIKMQKLLHRLITDVNDNNRHLLFIDPYIFFWLEKKFELWWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WCZ3</accession>
    <name>A0AAF0WCZ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Drought induced 19 protein type zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0206063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Di19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WCZ3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033347">
        <property type="entry name" value="Di19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027935">
        <property type="entry name" value="Di19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875:SF26">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14571">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Di19 zinc-binding" evidence="3">
        <location>
            <begin position="42"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Di19 C-terminal" evidence="4">
        <location>
            <begin position="129"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG86844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26187" checksum="0A55E7F5031AD215" modified="2024-05-29" version="1">MDADSWSARLSSASKRYQSALQSRSDIFMGFEEIEADDDIRDEYPCPFCSEYFDIVGLCCHIDDEHPVEAKNGVCPVCAMRVGVDMVAHITLQHGNIFKISFSADFLFLFFFSFCRKRKSRKGGSHSTLTLLRRELREGNLQSLFGGSSYLVPSTNVAPDPLLSSFIMPMVDDYGSGQSHSLAEVTSVKKNTTDSISERSIQSAPLSVKDHEERTRRSEFVQGMLLSTILDNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WEV4</accession>
    <name>A0AAF0WEV4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0207319</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF385">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="5">
        <location>
            <begin position="27"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG88086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1357" mass="153905" checksum="6B3B0FCAE7EEA417" modified="2024-05-29" version="1">MASTSSQSPSSSVSYLEPPPNSQRPHLLWDVFLSFRGADTRSNFVSHLYKRLADHGIRTFKDVPEVSTGEVIKKGLHQAIQESLIYVVVFSENYASSTWCLDELVEIYKNYKKMNRLPSVVRRQTGSFEEPFEEYEIRFADRKERVEKWRRTLEEVAGFSGQHISAERDEADIITEIVDRIEIEIKPRSLEVAAFPTGLETRVKDITTLMSCDTEGVTIIGMYGMGGVGKTTLAKALYNQLLDRRFKYCCFLADVRETAGRENGLVLLQQQFIDEFLRSKKQHKIHNVEEGTKFIRERFGSAKVMVLIDDINKLKHYKNLVPGIFGPGSVVIVTTRDEELLLKIEVEKRYRLYINLMEDDDSLALLSQHAFGNAVPEHALKFLLKDILSLAGGLPLALVVFGAYLSTRSEEGWKCYIEKLQRIPDSTIQQKLVISLDAIELEDPMLKKIFLDIACFFVGQRKREVVPILETYYSYVDHKIDTLKKRCLLTINNRYELRMHDLLRDTGREVARNRSYDEPGKHSRLWGSNIIREVLKRKKGTEAIEGIIPCKFNYHKALEGVSFVTKTFKRMRILRFLYLSGGNVTGNFEQTFANLRWLHWDHFPLRCLPSEFFPQELVFLALPSSKMRTFWELNMDSQVFEMLTTLDMSYSQDLVTTPDFTTLSSLKFLNLKGCKRLEEVHISIGCLLSLISLNLSGCVNLRSLPDNICNFRALKSLNVGGCSNLEELPMDLGNIESLTELNAWGLAVPKIPDSILNLSKLVELNLTDKEYLETPSTSLQDSTLTDWDVFLSYCSADTHFTSHLYTALDRHEIRTYKGDVELSTGEVIGLPQAFWESKFFPTIIANTVGDALTVLMLPESIGRLNKLVRLTLSFNKNLETLPNTICDLRALESLYIGGCSSVEALPLGLGNIKSLQVLDAEGLAVFRLPDSMGRLSNLVELTISYKKNLESLPDTICNLSYLKILNIRLGATLFIQPFDMKPSQFALSLQKLDLSESNITALTSNINHFLNLEYIDLINCGHLSSIAELPSSLKWIRAAGCASMESLPNVSNLKLLEKLDLTDCSALTEIQGLEEVSLLYKYSVWDLLREGSFEGSCFLTDVSEAWQTFKGPEILQQKLVNDVLKKDGEIKVHNVDQGTKLIRDGIISTKVLVVIDDLEDLNQYQSLVGPFVAGSVVIITTRDEEMLDEINVETKYRYMANVLDPAESLALFTQHAFDNGKLSRSLMVFCKDVLHLAGGLPFALQVFGSHLSSRSAGDWQDYIKNLQESPNSTIQQKLMISLDALALDDSVLKKVFLDIACFFLGSKMEEVLRIMGNRYHYLHDNICTLEKNCFLTINDRDELEMHQLLRDMARESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WG27</accession>
    <name>A0AAF0WG27_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0207129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG87896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WG27"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG87896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="118" mass="13838" checksum="413B4F24C854ABDC" modified="2024-05-29" version="1">MLDDLDPKKIYIVGGLVDRNRWKGITMKKAEEQNIQTAKLPIGNFLKMSSSQVLTVNQVIEIILKYLETRDWKDSFFQVIPQRKRFEADSEEKQGDLVDDKDEQQYDVQEKKKPCTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJI7</accession>
    <name>A0AAF0WJI7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pectate lyase superfamily protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0209745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJI7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF50">
        <property type="entry name" value="PECTIN LYASE-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042291851" description="Pectate lyase superfamily protein domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG90501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="51132" checksum="6F0AFA9CDDCBE724" modified="2024-05-29" version="1">MKTIWVLVVLMVGVVNLRGAECRKAKKRVENTLEYSAISCRAHSASITDYGGIGDGKTSNTKAFREAISHLSRYEADGGAQLFVPPGQWLTGSFNLTSHFTLFLHKDAVLLASQEISEWAVIAPLPSYGHGRDTAGGRYISLIFGTNLTDVVVTGENGTIDGQGALWWQQFHKKKLKYTRPYLIEIMHSDNIQISNLTFLNSPSWNVHPVYSSNIIIQGITILAPVTSPNTDGINPDSCTNTKIEDTYIVSGDDCIAVKSGWDEYGISYGMPTKQLIIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAINTESGVRIKTGVGRGGYVKDIYVKGMNLHTMKWVFWMTGNYGSHADSHYDPKALPVIQGINYRDITAENVSMAARLEGISGDPFTGICISNATIGLAKKAKKVPWTCTDIAGVTSGVLPKPCSSLPDQGPEKVGMCSFPTDNLPIDDVEIKKCSYRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WJI8</accession>
    <name>A0AAF0WJI8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-galactose/UDP-glucose transporter 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0209522</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. UDP-galactose:UMP antiporter (TC 2.A.7.11) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG90279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WJI8"/>
    <dbReference type="KEGG" id="dcr:108208478"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046964">
        <property type="term" value="F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF8">
        <property type="entry name" value="ADENOSINE 3'-PHOSPHO 5'-PHOSPHOSULFATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG90279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39474" checksum="7680D3AAE5742493" modified="2024-05-29" version="1">MKTNEDQDRFLFGISLSHRPAWQQFLICSSGFFFGYLVNGICEEYVYNRLQFSYGWYFTFVQGFVYLALLYLQGFTPKQMVNPWKTYVKLSAVLMGSHGLTKGSLAFLNYPAQIMFKSTKVLPVMVMGAFIPGLRRKYPLREYVSALLLVFGLIIFTLADANTSPNFSVIGVVMVSGALIMDAFLGNLQEAIFTTNPETTQKEMLFCSTVVGLPFLIPPMVLTGELFRAWKSCYQHPYVYGVLVFEAMATFIGQVSVLLLIAIFGAANTALITTARKAVTLLLSYLIFTKPLTEQHGLGLFLISTGIVLKMIPENKASTRIPISNVNNKLEKAYLIEEKRPAKTIEEASHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WPM0</accession>
    <name>A0AAF0WPM0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0312761</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WPM0"/>
    <dbReference type="KEGG" id="dcr:108214368"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="34"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="224"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG93477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="516" mass="59551" checksum="F3B245D54F7E3698" modified="2024-05-29" version="1">MASDTWTLEKRAPLRSDSVLKEVNVPEIGCLSIVVLGASGDLAKKKTFPALFNLYRQGFLQSQEVYIFGYARTKISDEELRDRIRGYLVPSKNTAEGHEEDLSNFLQLIKYVCGSYDTEEGYQSLDKEIAEHENLRNSKEGSSRRLFYLALPPSVYPPVCKMIRNCCMNKSDLGGWTRIVVEKPFGRDLESAEQLSNQIGELFEEPQIYRIDHYLGKELVQNLLVLRFANRFFMPLWNRDNIDNIQIVFREDFGTDGRGGYFDQYGIIRDIIQNHLLQVLCLVAMEKPVSLKPEHIRDEKVKVLQCVIPIKDEEVVLGQYEGYLEDPTVPDDSHTPTFATMVLRIHNERWEGVPFILKAGKALNSRKAEIRVQFKDVPGDIFKSNKQGRNEFVIRLQPSEAMYMKLTVKQPGLKMLAIQSEMDLSYGQRYQDITIPEAYERLILDTIRGDQQHFVRRDELKAAWEIFTPLLHRIDNGEFKSIPYKRGSRGPEEADELLKRAGYVQTHGYIWIPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WUJ9</accession>
    <name>A0AAF0WUJ9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0415687</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WUJ9"/>
    <dbReference type="KEGG" id="dcr:108217557"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1193">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPP13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="168"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="427"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="541"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG96352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="889" mass="102385" checksum="30D04DE5BDD885DD" modified="2024-05-29" version="1">MAEYIVAFVLDKIAGFLIEEAGSFARIDVQVEWIQAELRRMQCFLRDAELKQDGDERVKNWVSDIRDIAYDTDDLVDFCILKLSHQRSGFLYFLKKFTFYNEVLFRRKVNKHIDKIKKRIIYVKDSRSTYGIENLKIGNEMISFCGNRLNEKRRSFPYNNEDDVVGLEEDIEALMSRLIHGDCRRSVISIIGMAGLGKTTLAKKLYLNCDINKHFDCCAWVYVSQDYRARDILLELGKKVMGFTKESVEKMNGEDLLDSLCVFLSDKRYLIVLDDIWKHEHWDDLRPAFPEMSNGSRIILTTRFKDVALYANVRDPPHELCFLNNDRSWELFLRKLCVEWDSSSRLPPWSEDLGKQIVKRCGGLPLAIVVLAGLLSRKGLVFSEWQKVLQSVHWQLKQEPTQCEEILALSYSDLPYYLKPCFLYLGLFPEDFEISARRLTLLWVAEGLVQPRGEEHLEDVAEDYLEELIGRSMVQVAKRKSSGRTKACRVHDLLRDLCLARGQEEALFNVICAGDRVGSISGARRLAIHSGVPIFTRNSQKIRSLLCFDLIECVPLNLTKFKLLRVLDLEGVQIAQLDSAVGDLIHLRYLGLQETWLKNLPSSVGRLSNLQTLDLRSTLIEPIPLAIWKLQQLRHLYFSKFKAMVVCPPYRLTLPNLHTLKGICLSESGCIQNGLDNIVNLRELGLYGDLHLQEEEVSRWIHINKNLEELKIQSSNNLSYISNAAIPKLMSFSSHVRLYKLHLEGIISKMLDIKDFPPNLTELSLKDSFLAEDPMPILEKLQSLRVLKMKQTSFLGKELVCSSGGFPQLQYLKFGFLTFTTWTIEEGALSNLRQLEIVECKRLKISPKGLRPVTTLRELKLGFMPVDMKSKVQDRQGENWYKIQQVLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WW93</accession>
    <name>A0AAF0WW93_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0415157</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WW93"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000059">
        <property type="entry name" value="dual oxidase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF54">
        <property type="entry name" value="RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN J-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="507"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG95828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="852" mass="97245" checksum="A8C902A1D93888E7" modified="2024-05-29" version="1">MDNLHTRRRPGDSEKVVLQGVKIDKLNNEQNNMASPQSQNQTRQTLPKSVSKSTFGRIASTFKASRNASKGIGHRHAPHSGALRGLNSVRFIDKKQVGKEGDGWHNVENRFHQMAVNGKLSREKFGVCVGMGESRDFAVELFDTLARRRDVNTENGIDLEQLKLFWEDIALQDSDTRLSIFFDMCDKNGDGRLSEEEVKEVLIMSASANKLGTFKKHAGRYAAVIMEELDPDHLGYIEMWQLEAILRGGLANQEGGNSGGAAMKKSQSLARTMIPTRYRGAIGKYGSLALEKTYENWQKIWVMLLWLYVNAQLFAWKFQEYKKKPSFLVCSYCVCFAKGAAETLKFNMALILFPVCRRTLTKIRVAIGVAVHTGFHLTCNLVRMSSCSRSLFMSTLGPSFDYKQPSYMDIVMTIPGITGVLMILIMAFSFTLATHAFRRNVVKLPWIFHHLAGFNAFWYSHHLLAVVYILFYIHGTMLIIGKEKTTWMYITVPILCYATERLLVLRDLNFDVQIIKAIIYTGNVLALYMSKPPSFKYQSGMYLFVKCPDISPFEWHPFSITSAPGDDYLSIHIRTLGDWTTELKDRFAKVSMVHFYIRSELKLDGLKIHTPPYESTGLIINFFPTIIIKGPYGAPAQNYKKYDVLLLIGLGIGATPFISILKDLLNNIGDYKKQVSQMLDDFQLSRSEIWIHGQKVNSEHFRLLQDFDEEADAKIPDRAYFYWVTREQGSFEWFKGVMDDVAEQDVNHIIEMHNYLTSVYEEGDARSALITMVQSLERAKKGVDVVSNSKIITHFARPNWRKVFTDLATTHDKHIIGVFYCGSPTLTKPLRELCEEFSLETSSRFEFHKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WWI8</accession>
    <name>A0AAF0WWI8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
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    </comment>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
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        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
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    </dbReference>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG96934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="333" mass="37608" checksum="FCEA437F81670CC8" modified="2024-05-29" version="1">MERDESSSESFPMGETAQEKAFSHVPQCYTISTSQRPGLNPETGIVPVVDWAALSSPDRRSDAINEIKNACSRSGCFQIINHGISQSVLDGALETAFNFFKLPTKEKAKFMSNDVHKPVRYGTSLKDGQDKLQYWRVFLKHYANPLNQWIQQWPTNPPEYREQMGTFATEVNKIAAELMKAITESLGLGPTYLTHKMENGMQVMVINCYPPCPEPTLSLGLPPHSDYSCFTIVLHSSQGLEILDKSDDTWKVLSNGIYKSMVHRVALNRDETRISIASLHSLGMNEKMETAKELIDDEHPKGYRESSMMDFLNFLSKRDISEGSFIETLKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093346">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR039647">
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    </feature>
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        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG96821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="151" mass="17116" checksum="137A7915E18625B8" modified="2024-05-29" version="1">MDTSRSSLYLQDHDEIKRVFDRFDTNSDGQLSSDELAEVLKNLGTAPSPEEIARMMEEIDTDKDGFISFDEFERFYSAGSDNGEGEMREAFEMYDINKNGLISSSELHQILTKIGEKCTEQDCVNMITSVDADGDGFVNFEEFKTMMSRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3U4</accession>
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                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS07G0624100 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH00042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="87" mass="9976" checksum="BBA6303E93052A8F" modified="2024-05-29" version="1">MAASMKEKYDKYWGNADNINPLLFVAVVLDPRYKMRYLKYCFEAVYDSATVARLIVKVESVLQSLYTCYNVGSKGADNQVSYFIQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X493</accession>
    <name>A0AAF0X493_DAUCS</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
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                <person name="Sanseverino W."/>
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                <person name="Moranska E."/>
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                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
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    </reference>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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            <tissue evidence="4">Leaf</tissue>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
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    </reference>
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        <text evidence="10">Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents.</text>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="11">
        <location>
            <begin position="85"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH02244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="315" mass="34781" checksum="FD63DD914D6AE0A0" modified="2024-05-29" version="1">MAPLKSLEPDYPIIDSNFQQFCASHHIFSVEDFLLHDIYALELSTKQHFDSKRLEEGISVVRTIMEGLHQPWLNGMELLQKAQGNKHGISTGYERIDVLLEGGLFQGNLVELVGPSSSGKTQLCLKVAANVAVNNLGSVTFFDTGNSFSPKRIDKLLDQSSEPAKIKTEEVRRRVMDKIVCQSVFDIYVLFDLLHQLKSTLKPVTGSSVQMLIVDSISSLVTPILGSGAHGHALMVSLGFLLKELAYEHNLSVLVTNHMVGAEGGHLKPALGESWKNVPDMRLHVSRDCKSNICSMSILRHPYITAGKAVMFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9I9</accession>
    <name>A0AAF0X9I9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0522329</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9I9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF211">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH02939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="58174" checksum="E5B59B65014CA0AF" modified="2024-05-29" version="1">MAFVFLILLPTLAMILYFFYSVMWVPWRIQQHFRKQGVDGPNYRPFYGNTAEMTRMTKEALSKSISCLNHDILHRVSPDYYQWSAKYGKTFLCWFRMRPRLVLVDPDMIKDVMLKTTDTNDRDDFNPLSKLLFAQGLPGLRGHTWAVHRKIATPAFNKEKIKVWVPGMVASVTKMLENWEAKIGERDEIEMEVHKEFHELSAEIFSRTVLGNNYENGKRIFKLQQQQEISTNLAMQNVYIPGFRFLPTRMNRLRWRLEKETRDTMRMIIEASRKTSVNSLNFLSMLMSGDINKHGPRLEIEEVIDECKTFFYAGKEATANALTWALLLLAQHQEWQNKAREEVLLVCKDNEHPTVETLPELKIVSMIIKETLRLYTPDNSICRRTFKKVKVGSLDIPAGTELCVPQAAVHHDTKIWGADANVFNPARFEKPPKHLGAYFPFGLGSRICIGRNLAMVETKIVLAMIIKQFSFEVSPSYVHAPMMFFTVQPQYGAHILVKRINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X9S5</accession>
    <name>A0AAF0X9S5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mechanosensitive ion channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0623220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH03820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X9S5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF16">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="12">
        <location>
            <begin position="593"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="353"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH03820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="775" mass="88642" checksum="3D9666E6D61782B1" modified="2024-05-29" version="1">MADLEKNQSASAGRPPRIMENNRSKVSFQTPSRKFSEDENDNPRSNTPGAAEDSVSGHCYSRRLPSNAGGDRDEVIKCTSFQKRPSFLLSTKTVSRLSDPPVSDRRTDRVPASGPLKSGKVSGMLEKVPGIDDDDDDDDILLDDVPSDDFKTAKLDALTLLQWISLFTIVSLFICSLVIPKWKRAEFRGLELWKWEVLVLVLICGRLVSGWGIRIIVFFFERNFMLRKRVLYFVYGLRKAVQNCIWLGLVLIAWQCFFDQKIEGNNEFLWFINKLMWCMLTATLLWLLKTLMVKVLASSFHVSTFFDRIQDSLFNQYVIEMLSGPPLIELQNNQDEDERTMIEISKLQSAGATLPPDLRSAIPSPAAKSGRGSQTPGRSETDSKKFSGALTINSKRQEETITIDHLHKLNPKNISAWNMKRLMNLVRHGSLCTLDEQIQDLTNEDDNNKQITSEYEAKLAARKIFKNVARPRSKYIYMSDLLRFLGEEEAMKAMAILEGSSDSDRISKKDLKNWVVNAFRERRALALTLNDTKTAVNKLHHMVNVLVGVIIFIICILILGLASTQFLLAVSSQIVVVTFIFGNTCKTVFEAIIFLFVIHPFDVGDRCEIGGTQMIVEEMNILTTVFLRYDSQKIIYPNSTLSTIPIHNYYRSPDMDDAIDFYLHIATPIEKINLMKQKILSYIENKKDHWYPNAILVTRDTDQLNRIRMSLWPTHKMNHQNMIERYNRRSLLVEEILKTVKELGIEYQLYPLDINVKNMADITSTRMPSTWTSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEP2</accession>
    <name>A0AAF0XEP2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0625147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEP2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH05727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="143" mass="16956" checksum="79B094441125249A" modified="2024-05-29" version="1">MFYRRLIRWLTGSIDSLYMFSYYLCFSVVLENRTPEFMAPEVYEEQYSELMDIYAFGMCILEMVTFEYPYSECFYAAQICKKVVSDPEIREFIEKCLATASCRLPERNSLMICIFNQRLKSELLTSNHPLLKRVLITVEGIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEV3</accession>
    <name>A0AAF0XEV3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0625183</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SHL1/EBS protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEV3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04714">
        <property type="entry name" value="BAH_BAHCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000561">
        <property type="entry name" value="Bromo-adjacent homology (BAH) domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000019">
        <property type="entry name" value="Chromatin remodeling protein EBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0287">Flowering</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="13">
        <location>
            <begin position="21"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="138"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOH05762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24330" checksum="0D576308385F6F63" modified="2024-05-29" version="1">MAKTKPGKKDLDTYTIKGTNKIVRPGDCVLLRPSESDKLPYVARVEKLEADHRSNVKVRVRWYYRPEESLGGRRQFHGAKELFLSDHYDMQSAHTIEDKCIVHSFKNYTKLENVGVEDYFCRFEYKAANGAFTPDRVAVYCKCEMPYNPDDLMVQCEGCKDWFHPSCMGMTIDEAKMLEQFLCGECASEEDAKRSSNTFPVPEPSTEAKVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFF2</accession>
    <name>A0AAF0XFF2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0625500</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Vesicle trafficking protein that functions in the secretory pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of the t-SNARE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFF2"/>
    <dbReference type="KEGG" id="dcr:108225091"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000037">
        <property type="entry name" value="Putative syntaxin-71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="170"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH06077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="264" mass="29846" checksum="F973FCD056A5093A" modified="2024-05-29" version="1">MTVIDILTRVDVICKNYDKYDIDKRNHLTVSADDAFARLYADVESDIDSALQKAETAANGNNRASVVAVDAEIRRTKARLLEQVPKLQKLAMKKVKGLSPEEFVARNDLVLALPDRIQAIPDGTPAVPKHTGGWTASASHTEIKFDSDGRFDNEYFQQSEESNQFRQEYEMRKMKQDQGLEMIGEGLDTLKNMAQDMNEELDRQVPLMDEIDTKVDKATSDLKNTNVRLKDTVTQLRSSRNFCIDIILLCIILGIAAYLYNVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XFW6</accession>
    <name>A0AAF0XFW6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0625492</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XFW6"/>
    <dbReference type="KEGG" id="dcr:108227451"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000465">
        <property type="entry name" value="Heat shock cognate 70 kDa protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000005">
        <property type="entry name" value="heat shock cognate 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
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    </dbReference>
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="644" mass="70816" checksum="74A18C8695152FEE" modified="2024-05-29" version="1">MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDIKLWPFKVTPGPGEKPMITVSYKGEDKQFAAEEISSMVLIKMREIAEAFLGTTVKNAVVTVPAYFNDSQRQATKDAGVIAGINVLRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEEMNMDLFRKCMEPVEKCLRDAKMDKSTVHEVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLITRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYSYNMRNTVKDEKIASKLSADDKKKVEDAIEAAIQWLDTNQLAEADEFEDKMKELESICNPIIAKMYQGGEGSAPMDDDIPSGGGAGPKIEEVD</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
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            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: A02"/>
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    </dbReference>
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    </dbReference>
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            <dbReference type="EMBL" id="WOH07615.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="78" mass="9002" checksum="F553F61190E34FD1" modified="2024-05-29" version="1">MKARQNIMEQNKSSQEEKYLGVAVHSQVRKIKQEMEKIEQPSLEEPEFLVGPVFDENRRQRSRSPLGIAHRPISVGNS</sequence>
</entry>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
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        </lineage>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="12">Leaf</tissue>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BZIP family transcription factor"/>
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        <property type="entry name" value="TGA_domain"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
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        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
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        <location>
            <begin position="107"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="61"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH07903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="326" mass="36255" checksum="5157A87DB5411CFD" modified="2024-05-29" version="1">MADASPRSDSSTDGDTDDKALGFHNSQGVVVSDASDKSRDQKTLRRLAQNREAARKSRLRKKAYVQQLESSRMKLTQLEQELQRARQQGIFISSSGDQSQTMSGNGALAFDVEYARWLEEHNRRVNELRGAVNSHAGDAELRIIVDGVLAHYDEIFRIKGDAAKADVFHILSGMWKTPAERCFLWLGGFRTSEILKLLISQLEPLTEQQLLSISNLQQSSQQAEDALSQGMEALQQSLADTLAGSLGPSNSSENVANYMGQMAMAMGKLGTLENFIRQADNLRQQTLQQLHGILTTRQSARAVLAISDYFSRLRALSSLWLARPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMD5</accession>
    <name>A0AAF0XMD5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoprotease 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GCP2</name>
        <name evidence="10" type="ORF">DCAR_0730228</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMD5"/>
    <dbReference type="KEGG" id="dcr:108193250"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000105">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="9">
        <location>
            <begin position="32"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="352" mass="38446" checksum="57E3F5FB97411A2A" modified="2024-05-29" version="1">MKRLLALGFEGSANKIGVGVVSLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLIKSALEAAEVTPNEIDCLCYTKGPGMGAPLQVAAVVVRVLSQLWKKPIVAVNHCVAHIEMGRIVTGAKDPVVLYVSGGNTQVIAYSEGKYRIFGETIDIAVGNCLDRFARVLELSNDPAPGYNIEQLAKKGEKFLDLPYAVKGMDVSFSGILSYIETTAEEKLKNKECTPADLCYSLQETVFAMLVEITERAMAHCDKKDVLIVGGVGCNERLQDMMRTMCSERGGNLFATDDRYCIDNGAMIAYTGLLAYAHGLSTPMEESTFTQRFRTDEVLAVWREKESSNMNSVSGEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSR5</accession>
    <name>A0AAF0XSR5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0831628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XSR5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13960">
        <property type="entry name" value="PT_UbiA_HPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044502">
        <property type="entry name" value="AtHST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009525">
        <property type="entry name" value="PRK12887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009:SF7">
        <property type="entry name" value="HOMOGENTISATE GERANYLGERANYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009">
        <property type="entry name" value="HOMOGENTISATE SOLANESYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH12129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="396" mass="44048" checksum="9D8A0B51F88DB9DD" modified="2024-05-29" version="1">MIQSFMPSCFSSSFHVQPEKALPSQRRHAKILNQDKKYKYTGPSSSNNIIKAREILSSSCKLKAYKNQRLQTFCTTSDAEFLLHPNDNRKATPKSRLWEEWDAFYRFCRIYTVNGMVAGVSSVSLLPLTSVGDLSPAFFVGMLQALISFVILNLYVVGINQLADVEIDKINKPHLPLASGEYSMEKGITVVSALGFMCLAMGIISWSPPLFVGIVAYILLGTVYSIKLPLLRWKTNPYLAAFVIVGLHGLTIQPAVFYHIQHVLGKPFTLSKSLVFATIFFSLYSVVIALFKDIPDVEGDRAFGNRTFSVRHGKKKVFSICGGILLFAYGSGVVVGASSSFLICKIVSVIGHSALASLFLLRAKSLNLDDDVATQSFYMFLWKLFSAEYVLIHFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XVB4</accession>
    <name>A0AAF0XVB4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trehalose-6-phosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0933507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XVB4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000079">
        <property type="entry name" value="Trehalose-6-phosphate synthase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF14">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00982">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH13992.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="708" mass="80227" checksum="093E18864FF3E52B" modified="2024-05-29" version="1">MDTNSRMNFANLAFGDVDTGTNSCVKYVDSDGTSSMAVRNIIVSNMLPLHAQKDWETEDWSFNYDEDSPLWELKDGFSPDIEEVAKRLLDDFNCVPTFLTNDLLEKSYHGFCKHHLWPIFHHMLPMCGKHGDRFDQGLWQAYISANKIFADKVLEVANPETDYIWIHDYHLMAVPIFLRNKRHRAKLGFFLHSPFPASEIYRTLPVSADLLRSLLNCDLIGFHTFDNARHFLSCCSRMLGLDYESKRGQIGLHYSGRTVYNKILPIGIHLGKGENVMNLPSTSVKVKEIEEQFKGKHVILGVDDMDLFKRISLKLLAFEQLLSKYENLGDIVVLDIEEVRREAYGTANRINHIYGSCGYQPSAYYAASECCIVNAVRDGMNLVPHMYIVCRQGSTAMDEARGIISHSRQTSVLIISEFVRCSPSLSGAIRINPWDISSVAEAMRSAVSMDDSSRQLRHEKNYSYVQSHDSMERSCLDHYNRQCWGMGLGFTFKVAALPLDFQKLTNKRAIFLDFDGTLVPHSSTNKKLIHEVVTALNTLCDDPKNTVFIVSGRGRNSLIEWLAPCEGLGIAAEHGYFIKWKKTSEWQSNFVVDLHWKAIVERIMNLCTEATDGSTIEVKESALVWHYQDADHDFGSFQAKELPDHLKHVIANELAKVKKGTYIDVSKSLVTQKVISTMVGNGGIPDFILCIGDDRSDEDMFEGPLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Enod93"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG81420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="136" mass="14809" checksum="2B0C673AB99F491C" modified="2024-05-29" version="1">MCPSVLNAGYTDEIGGLNIASLECLSGIARAYLSPHYPAAMLLPDYLNFCNFHNLHFAYGDADVQNAVFAGLKSLGITAGVTYASVTLANRHIPWARKNLNYTGRTLIVCGGTVAVHVRSDYRSSDLRNPEEKYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJ46</accession>
    <name>A0AAF1AJ46_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104462</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AJ46"/>
    <dbReference type="KEGG" id="dcr:108203763"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11446">
        <property type="entry name" value="bHLH_AtILR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000104">
        <property type="entry name" value="Transcription factor bHLH34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044818">
        <property type="entry name" value="ILR3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133:SF23">
        <property type="entry name" value="TRANSCRIPTION FACTOR ILR3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="76"/>
            <end position="127"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
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        </location>
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        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG85274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="235" mass="26360" checksum="1BAFABFADC7A1928" modified="2024-05-29" version="1">MVSPENNPNWMFDCGLLDDVPVPGGHLPSLEPRFQWPTNAFPDSTVLSAEFVESFGNSDGIKEHGSRKRVKSGSCSASDSKACREKMRRDRLNDRFQELSSILEPGRPPKMDKCVILSDAVRMVNQLRDEAKKLKESHEELQDKINELKAEKNELRDEKQKLKAEKEKLEQQVKGLHPQPGYMPPPVPSPFPGPGQFFGGKMVPFMGYPGVPMWQFTPPAAVDTTQDHVLHSPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJX4</accession>
    <name>A0AAF1AJX4_DAUCS</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">DCAR_0102123</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ANZ2</accession>
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        </recommendedName>
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    </gene>
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            <taxon>Spermatophyta</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
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        </reaction>
    </comment>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25110">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25113">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="5">
        <location>
            <begin position="1903"/>
            <end position="1997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG87106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="2133" mass="240931" checksum="6D4A4463A60D610A" modified="2024-05-29" version="1">MDNSPEASDLLSKLESSPDFRNFYTLISSYLLPFTSTLTTSKPSKSSKRVVDNTRALAKQFLPFLNRCLSLIPKRLLSQCPKLDSSSALQLFDSYVVCLNCMDSVSSQLSGKAYAVHLQRVRLVHCYLFWERYGDASREGLSLLQSVCRLGGDGNRNEYVPDMTRENEKDHDFAWLVVEIVVSLVKCVAMDQSKVESDYRRLLVMISEVTPWFRILDANAYEKLHRVHVSYMSKCALILVDDLNDFDDKVARRFCLATFAELAKSSSKDQLFKFAHRLCYSLFSKEKNPSSVVDILACVLDSVANAGEVGTQTSTELLELVSYCATKSLYASSDMSFSVAEQLQKFADDICLVSPSPTPLLTSLYATGLFFSDFCGQTMAKGSTSSRTDKDSSIYGFLLYYEHRLQELTGYLQSLKHYYNSTKRISLHEDPDLVSYFNALKFLCKPLAEFVNSERGTIVTEIVDATSAAKLKYIQDALHQYLEIFILYQRGSEKKREANDGNNKTTLCVAVAAFILSVKRNQGIKESTTFIKNSISSDWIQFHGLKFLFSSLYNFGVVLYKNNNTKEASLSLKLCCRASWTCLLNNCKLFEDKSIEFSDEMSEDSIIGLLAEACTRSAFVLDVLYQCDCNKMNKLITYSLEKWAAAEKLFDRLPSPTALLKQWVKIQCKISKTDDVENNGKTLYSVLSSSVKMSKRAPGILLEQELLLYEEMRPLNPTFCRRMRMKIVDILLKEVYVTKTIQRSRVLIAKGRELRACGVERLDDCIECLSESISMMSETNRSIHCDSIHSYYLANAYCLRALCTQEAEPDRKVIFQDMDAAIKLWMSIDLPCLSGQIDMVFENILFLLYHVVDLLSLKGYMKFHANIYEIIIRLFEWRNVPVKKLLAILWECRRLGHALCASPVNETFITTLSNHCDASNTMEYWLSCLTESPLLEVGFKQNFSYMFTDFSLVAHHHVSSQAEITIDGVEQAVSNLLSSVPLSSGSASIAAHMYYDLGERLISNGQFIEALSYAKEAYQIRTKLFQEKFMYKIDQQSEVCNGTGEVLQKQCYSLKTLDIFSSVATAAWSSECVTRDFDSHVVTPWNVLQRYLESILQVGSIHEIVGNGSDAKRLLLWGKNISCFQDLPLFTISFSSVLGKLYCKEQLWEFAELELQKARHLLDKLDSWSPFYCCKCKLVLEVTIDQQLGDLSRGRCDDSNIGELSVDRLLYAEKRYRIALETLRFSEWKNSVSNPEETAVSGTGTSFSLQSNYLETKISFECCNREETKVDQEISRKSRKVAKILPKGQCLTRQQNRMMTRSCRKNIECDQEEKSKNKSKFPSSDAVHKNTKIVNLGSRASKEIKCWYCLPHDVSESGYLIDYIHMKWELVRRRLLLRVLTSIGKCSVIQNERQKGDEIFSESISVLVARNQFSPTYQSVPFSLKLDLIGSDIPGDALAVERATILYNLCWFILKYNHCKGTSVGSDFSGITISRIMAWLKLAFLICREVPVLFRKVSRLLAVLYICSSVKILSLPSSHSDTITASQWGSYFHQASLGTHFNQQLFSHKAGKQKGESFSKTKGCLSTSSSLGQETANSVRRTPDILLREFVSNFYQSLPPATIICISLLGVAYASLLRELSSSPASVRAWILFSRLKSDGQPVFLLLPADSILGEASGDETTSLGFLHESQSSVKRWHCPWGSTVVDDVAPVFKLILEQNYLSSSAYPLEDTKMTRSLWWTQRRKLDQYLGHFLRDIEEKWFGPWKFLFCSDWSDCKHLDLAINKLADDLKVKCDVNLHESLLRVILAGGQHACDSSRCVPNLILNNGCHVDGVECNIDEMSMVFKLILETIHEFEEDLCVNREPIILVLDHEIQMLPWESLPILRNQEVYRMPSICSIFASLDRHDHKKLGTGSMVFPSIDPLDAYYLLNPSGDLISTEVEFDKWFKDQNLEGKTGMTPTIEELTGALESHELFIYFGHGSGAQYIPNHEIQKLRTCAATLLMGCSSGSLSLSGSYSPQGAPLCYLIAGSPVIVANLWEVTDKDIDRFGKAMLDAWLRERSMDQASCAQCDAITDELKSLSISAAKGKGKKKTSRKILSAICDVSKCNTCRHRPKIGTFTSQAREACTLPFLIGASPVCYGVPTGIKKKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AQS6</accession>
    <name>A0AAF1AQS6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0208080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AQS6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000127">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF132">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MPSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="106"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG88845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="205" mass="22753" checksum="25AEC4069E7029EB" modified="2024-05-29" version="1">MPQVTPNDQLNNSRTTDLSQNTPITTDSTDSQTNTDPDQDSSERSDPRDRIILLNPLTRRVVVIEGSASRIDALVRNLTGPTGPPPASKASIEAMRSVETKEDDECVICMDEFVGGLAKEMPCKHKFHGECVEKWLKINGSCPVCRYKMPEEDDNDFNHKNPFEGEEVRRRGSFVTISVGTLRVEPNQMATQGSDGQDSEIQSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASV9</accession>
    <name>A0AAF1ASV9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0310992</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASV9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000062">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF58">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042020799" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="507"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="243"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG91742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="788" mass="85742" checksum="F80E89EB20B86C75" modified="2024-05-29" version="1">MSNLNLVYIFLELILIFEAGTSYGFTNPVDVAAINSLYIAVGSPILPRWTSVGGDPCGDAWQGVECDGSNISSIKYNVANMNGDLGDNLGSFASIKIIDLSNNQIGGTIPTNLPVSLQQLYLADNKFTGSIPDSLSSMNQLSAMSLNGNQLTGDIPDSFQGLTVLVNLDLSSNKLTGQLPPSLEGLSSLTTFRLQNNQLSGTLDVLQDLPLRDLNVENNSFSGPIPEKLLSIAIFKSDGNPFNTSVAPSAPPTSPTGSPTLPTARPPAPPFFRVPASGQTPEKAPGNETPSRPADGPSATTDANSKKTNQSSNTKKIVWISIGSVFSVVILLLLLVLLIPRCCKERGDRVPRRYEIAPYIGNRENLREYGSLEQPFHQTEKGPKVDLVKPQDNYLKGPIVSSLAPMPREELNVDKMSAVPKVEDHEIDMTRFDFDSMLPPPPPPPPPPSPPPQLAPPAPPPMPLKSVTVNPILPAQTIPVNSSSGPLPPTTVRSYSIASLQQYTNSFSQENLIGGGMLGNVYTAQLPNGKLVAVKKLDRRVSSQLKDDKFLDLVNSIDKIRHANVVEILGYCAEHGQRLLVYEYCSNGTLQDALLADDESKPSWNLRIRMALGAARALQYLHEFCEPPVVHRNFKSSNVLLDDELTVHVSDCGLAPLISSGSVSQLSGNLLSTYGYGAPEFESGIYTSMSDVYSFGVVMLELLTGRKSHDSTRSRTEQFLARWAIPQLHDIDALSRMVDPSLQGKYPVKSLSRFADIIFRCVQPEPEFRPLMSEVVQDLLQLIRRDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AT72</accession>
    <name>A0AAF1AT72_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">DCAR_0313734</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AT72"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042065072" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="20"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="349"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOG94438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="663" mass="74401" checksum="F61828A461B7884A" modified="2024-05-29" version="1">MLAELPFLLIQLMYTLVSADQEFHFNGFQSNNISLGGVAIITPNGLLKLTNSTFVAGQAFYSNSITFKNKDSLKNGTNSSLVYSFSTTFVFAIVPKYSQVSAHGMSFVIAPTKDLSAAAPSEYLGIFNPTSNGNASSHVFAVELDTFRNLQFQDINDNHVGIDINSLNSSVSRPAGYFDDVNEEFQNLTLPGGQIMQLWVEYSDLEKQINVTLAPIKIGKPKKPLLSLRYDLDKIINEEMYVGFSSTTGTLAFAAHYVLGWSFMVNGMARELELSKLPKLPRIGPKKKHIFLTIELPLILVIILLLTISGIVYYRWRKKKFAEVLEDWELEYGPHRFKYKDLYVATEGFDDKELLGIGGFGRVYRGVLPISKIEVAVKRVSHESRQGMREFIAEIVSIGRLRHRNIVQLLGYCRRKGELLLVYDYMPNGSLDRFLHNSPEIMLNWMQRFRVIKGVAAGLKYLHQDWDQVVIHRDVKASNVLLDIDMNARLGDFGLSRLYDHGTDTQTTHVVGTIGYLAPEHARTGKATTSTDVYSFGAFLLEVVCGRRPIDVRKEVLLVDQVCSLWNKGEIIQAVDHKLGVDYVVEEVELVLKLGLLCSCVDPNVRPSMQQVVQYLGGYEQLPELSDLSISQTGFTFENDTVDVLTTSTQSYSVVESFLSSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUA5</accession>
    <name>A0AAF1AUA5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0312021</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUA5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415:SF20">
        <property type="entry name" value="NDR1_HIN1-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="81"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG92746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="211" mass="23500" checksum="E00310FFE297CB25" modified="2024-05-29" version="1">MSRMDAKSPQHCAKKGFHIRKRYKKLLYTLCTILLSVISIAFIIYFILRPSKPEFSLKEIDINQLNLSSSPQLVNSSVELTLLSKNPNQRVGIYYDELNIYASYKNQQITVPTSLPPFYQGHGDNNLLTASLVGTGLPVAPSFGYEVKRDQTVGKLVLYLKGNGHLRWKIVSWVSGKYRFNVNCAAVLPFGPSIPSGPISSKQGTQCSTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW13</accession>
    <name>A0AAF1AW13_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="15">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_0313613</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="15"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW13"/>
    <dbReference type="KEGG" id="dcr:108213004"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010075">
        <property type="term" value="P:regulation of meristem growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000164">
        <property type="entry name" value="Os01g0740650 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PA" evidence="16">
        <location>
            <begin position="144"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="325"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="17">
        <location>
            <begin position="572"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="WOG94320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="704" mass="77594" checksum="E0B4436A4CBC38D6" modified="2024-05-29" version="1">MYTSIAATLITIASSLTYFFISSPQKSNYHSLYIASTFSDNVSVSQHLYSLTRRPHVAGTEANAEVARYVLSTLSSYDIRSHIRSYGVSLTYPVSRSLALTHPPSDTTTIFELRQETYEGDPYIDVADEVLPTFYAYARSGTVVGPVVYVNYGRVEDFMILKTKGLNVSGTVVLARMGQIYRGDIVANAYQAGAIGAVLFTDKKDYGGGDDGKGFPHDKWMPPSGVQVGTLYKGCGDPTTPGWPSTGGCERISDDEVDKGGDIPLIPSLPVSAVDGEAIIKSIGGEVADSDWQGCKDGPVYNIGPGPGILNLSYTGKQGINTIENVIGIIEGEEEPDRFVILGNHRDAWTFGAADPNSGTAALLEVADRLSKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLSSRVVAYLNVDVAVSGAGFQAAATPQLDQLLMQATKQVRDPENSSQSIFDSWISTSDHPKIGRLGGAGSDYAPFLQHVGIPAADMSFGEGYPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALSLADEEFLPFNYLSYAFELQTNADELTNELMDKNIDLTPLFKSIEDLKIAATKIDNEIKALKRSKGWASMWGTKPRQVRELNDRLMMAERAFMDRDGLLGRQWYKHLIYAPSEHDDYGSVSFPGITDAIEKAKQENTKESWSSVQHEVWRVSRAVIHVSLVLNGVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AX20</accession>
    <name>A0AAF1AX20_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
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        <lineage>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
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</entry>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Simon P."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </evidence>
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        <name evidence="23" type="ORF">DCAR_0520814</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B0F0"/>
    <dbReference type="KEGG" id="dcr:108220107"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000784">
        <property type="entry name" value="leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000676">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000150">
        <property type="entry name" value="serine/threonine-protein kinase BRI1-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001709">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF58">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE MSP1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041916909" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="19"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="903"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="991"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="1020"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="WOH01430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1269" mass="137711" checksum="763BEACB9FCE9DA7" modified="2024-05-29" version="1">MAPLFLSLLLLQLLCSHAITTTEFAERDSLLTFKHSLKNPAQVSSWTTKTSHCHWLGVSCVNNSVTQLVLPTHSLSGPLAISLFSLTSLTTLDLSNNQLSGELPSQLGELTRLEILKLGPNCLTGEIPPEIGKLGSLRQLDLSGNSFTGNIPEEIGDLFRLQSIGLSGNVLSGSLSPALFTNLNSLTFFDVSNNTLSGNIPPEISKLKKLTDLYIGINKFSGELPSEIGELSSLENFFSPSCLIRGPIPDTISKLRSLTKLDLSYNPLRCSIPRGIGKLENLTILNLVYAELNGSIPSELGNCRNLKTLMLSFNLLTGSLPEELGGLPMLSLSAEKNQLSGPLPSWLGRWDKVDSLLLSSNRFSGPIPPEIGNCSLLDHISLSNNLLTGVIPKELCNSGALTEIDLDSNMLTGTIEDTFVNCGNLTQLVLVDNQIVGPVPEYLSKLPLMVLDLDSNNFTGTIPMSLWNSMNLLEFSAANNHLEGTLPVEIGNAGSLERLVLSNNQLSGSIPGEIGNLTSLSVLGLNSNFLEGSIPVELENCISLSTLDLGNNQLNGSIPEGIADLEQLQCLVLSYNNLSGPIPSKPSKYFRQISIPDSSFIQHHGVYDLSHNRLYGSLPEELGSCSVIVDLLLSENLLSGQIPQSLSRLSNLTTLDLYGNQFTGTIPAELGSSQKLQGLYLGKNQLTGTLPESLGQLSSLVKLNLTGNKLSGYIPSALGDLEGLTHVDLSYNELSGELPSTLSSMVNLVGFYVQQNKLSGHINELFHDSMVCRIEVVDLSNNMFTGSLPQSIGNMSYLMSLDLHGNSFSGEIPSDLGNLMQLQYLDLSNNGLSGEIPKKLCSLENTVTLNLAENRLEGPIPRIGICQNLTNITVAGNKDLCGGLVGLRCPLKSFHKRSRLTSIWVVALIVVGGALATLFTTIVLYIWVHRSNKQSDLEESEDSKLNSSVNQNLLFLSSSRSKEPLSINVAMFEQPLFKLTLVDVLQATNNFCKTNIIGDGGFGTVYKATLPDGKIVAVKKLNQSKTQGNREFLAEMETLGKVKHENLVSLLGYCSFGEEKLLVYEYMVNGSLDLWLRNRTGALDVLGWSKRLKIAVGAARGLDFLHRESIPHIIHRDIKASNILLDKDFEPKVADFGLARLISACETHVSTDIAGTFGYIPPEYGQSWRSTTKGDVYSYGVILLELVTGKEPTGPEFKDVDGGNLVGWVYEKIKKGQAVDVLDSTLLDADSKPVMLKVLQIAAVCLSDNPYDRPTMHNVWKVLNGISEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B0N3</accession>
    <name>A0AAF1B0N3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0521567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH02179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108221736"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF45">
        <property type="entry name" value="SECOISOLARICIRESINOL DEHYDROGENASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="313" mass="33738" checksum="0C3EEE675481D658" modified="2024-05-29" version="1">MKASSLLAPFARRLEGKVAIITGGARGLGETAARHFLRHGAKVVIADIQDELAHSLCKDIDDKSSLSYIHCDITKDSDVEALVGITLAKHGKLDILHASAGISTSSRSESKILLANNEIFRNLMDVNVYGTFLSCKHAARAMIPAKSGSIILMSSAASVSSGPISHGYLASKHAIVGLTKNLGVELGKSGIRVNCLSPFIFDTELSRKEFGLDDEEAVKRFVSDFSNLKGVILDAEDVAAAAVYLGSDEAKYVSGLNLVIDGGYSTTNEAITQAIMRKNSYKLSRIETHYTLLGVCLRVLQLVFYVCFCFFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B0Z2</accession>
    <name>A0AAF1B0Z2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0521883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47340">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47340:SF1">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="846"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="1059"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="1063"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1034"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1452"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1034"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH02494.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B249</accession>
    <name>A0AAF1B249_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulator of rDNA transcription protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0622701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B249"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052997">
        <property type="entry name" value="RRT15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33047">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33047:SF8">
        <property type="entry name" value="REGULATOR OF RDNA TRANSCRIPTION PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH03305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17017" checksum="414B3657136CEC5D" modified="2024-05-29" version="1">MIGRADIEGSKSNVAMNAWLPQASYPCGNFSDTSSFKFRRSKGSLGHAFTVRIRTGNQNQTSFYPSVPHEISVLVELILGHLRYLLTDVPPQPNSPPDNVFRPDWPPTGSGLGPPCPALRANPFPEVTDPFCRLPLPTLFHRPEAVHLGDLMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B2P2</accession>
    <name>A0AAF1B2P2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0521737</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B2P2"/>
    <dbReference type="KEGG" id="dcr:108221040"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009805">
        <property type="term" value="P:coumarin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000357">
        <property type="entry name" value="Caffeic acid 3-O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000061">
        <property type="entry name" value="Caffeic acid O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="135"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH02348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="359" mass="39256" checksum="3F2841EC8868887E" modified="2024-05-29" version="1">MGGIKTGRSEDEEACLLAMQLATATVLPMILKSAIELDILNTMAKAGPGNYLTPSELASKLPRSNPDAPAMVQRILRVLATYKVLGCKPNDRSNGEAEWLYCWTPVCKFLSYNEDGGSMAPLLLVNTDKVVIDSWYHVADAVLDGGIAFNKAYGMSIFDYNSREPRFSKVFNQCMTGHSNITLKKILETYNGFQGLSSIVDVGGGSGATLNMIISKYPTIKGINFDLPHVVRDSPSIPGVEHVGGDMFTSVPNGDAIFLKWVCHNWNDEDCLRILKNCHQALADNKKLIIAEFILPEVPGGSDDATKGVVHMDSIMMAHVPGGKERSEKEFEAMATQAGFKSFSKVCCAFNTWIMELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4R2</accession>
    <name>A0AAF1B4R2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0522535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH03141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1B4R2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="3" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="1 3" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0793">Thylakoid</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH03141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="117" mass="12727" checksum="CF8AED61D3581FAB" modified="2024-05-29" version="1">MVSHLMRLCGSRPVSKSSLKVALTIYWEPKPCSCSEHSCIWSSQVVLMGLIEGYRVGGSPLCEGLDVIYPGGAFEPLGLAEDPESFSAMFGYFVQAIVTGKGPMENLYNSMTMLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1B4W5</accession>
    <name>A0AAF1B4W5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0625715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH10358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="7">
        <location>
            <begin position="80"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOH10358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43737" checksum="1A9E9E528603F6E6" modified="2024-05-29" version="1">MSAPSSVKKTETEALRRAPHEKPPFTIGDLKKAIPAHCFEKSLVTSFRYLIQDLIMAYALYYAATNYIDQYLPSPVNYLAWAAYIAVQGCVLTGAWVVGHECDHDAFSNYGWVNDLVGLIVHSSLLVPYFSWKISHRRHHANTQSLENDEVYVPRFRSNIRNYYKILNNPPGRVIVWVITLLIGFPIYLMFNVSGHKYERWTSHYDPHSPLYTERERKQIIVSDVAILAVIYGLYRLVLLKGFAWVFFVYGGPLLVVNGWFTLITILNHTHPSVPYYDSSEWDWLRGALCTVDRDYGILNKVFHNVCNAHVCHHIFSMIPHYHGLEATEAMKPVLGDYYQYDGTPILKAMYREMKECIYVEKDEGETKGVYWYRKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAB7</accession>
    <name>A0AAF1BAB7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ApaG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0730022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH10553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BAB7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050718">
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    </dbReference>
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        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47191:SF2">
        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="ApaG" evidence="2">
        <location>
            <begin position="130"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH10553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28896" checksum="125C23FF86167A43" modified="2024-05-29" version="1">MQSLSFKVFVDFNGGGTARLPCFPSRLGSVGADFEAGDRRLGLNYRDHGRTCRIVACSSGADSQSQSASSSSNSFLTRSQSYALLKQQMEVAAKSEDYEEAARLRDSLKSFEEEEPVLHLKRLLREAIANEKFEGIKVQVRSVYIEGRSQPLKGQYFFAYRIRITNNSNRPVQLLRRHWIITDANGKTENVWGIGVIGEQPVILPNTSFEYSSACPLCTPNGRMEGDFEMKHIDKVGSKTFNAVIAPFSLSTYGDGTDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BAT0</accession>
    <name>A0AAF1BAT0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0830527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell junction</location>
            <location evidence="7">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051378">
        <property type="entry name" value="Cell2Cell_Antifungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080">
        <property type="entry name" value="ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080:SF27">
        <property type="entry name" value="OS01G0548750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041945846" description="Gnk2-homologous domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="11">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="11">
        <location>
            <begin position="134"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH11048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="301" mass="33178" checksum="9BE8821FAC302196" modified="2024-05-29" version="1">MKSIMMLGHTFVIVSVIMKGITSDSQVEELLGKCTRLKVSVDLPIFLNSVNKTFEDMREQLSNAHFATTYETDVFGMAQCRNYLSTADCLACFDAGVTDIRRKCNAAVVGTHFIYQGCFLRYGIHNFYKEITLESPVLVCSYNHNIAEPGAFSPIVQGLLTEIVSATPKMKNYFAAAKRQVLSDGTTPTVYAVAQCVETISQSDCRSCLTRTYTNLKTCLSQPGGTCAEAGCFLRYSDSSFFADNNITNITPYIGGGSSSAKRPILGIIGGLMFLLLIIASIFWYQLVRKAKLEERAKITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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    </protein>
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        <name evidence="3" type="ORF">DCAR_0933468</name>
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    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the QWRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="dcr:108200379"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nuclear microtubule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="AUGMIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807:SF2">
        <property type="entry name" value="PROTEIN SNOWY COTYLEDON 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04484">
        <property type="entry name" value="QWRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="212"/>
        </location>
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        <location>
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        </location>
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        <location>
            <begin position="326"/>
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            <begin position="26"/>
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            <begin position="273"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Photosynthetic NDH subunit of subcomplex B 5, chloroplastic</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16132.1"/>
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    </dbReference>
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        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH16132.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="215" mass="24300" checksum="1911FCADFBA803E6" modified="2024-05-29" version="1">MATSVSVSSLNSVPRFGFGFKPDPCSSRARVLLQTPLSSVKHGRLSLKRLNAAGLADIEPDLNEDPKDRWATNGVDPDDFIYGEYDDHHTYHPGDHEKVSFWGSIAEDYAAIDPPTGFQGLISWLFLPAVAVAMYFDAPGEYIYIGAAIFTIVFCIIEMDKPSEAYNFEPQIYNLESKSRDKLIADYNTMDIWDFNEKYGDLWDFTVTKDDIMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH16326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BKT9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053340">
        <property type="entry name" value="PTF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48428">
        <property type="entry name" value="PLANT-SPECIFIC TFIIB-RELATED PROTEIN PTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48428:SF1">
        <property type="entry name" value="PLANT-SPECIFIC TFIIB-RELATED PROTEIN PTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH16326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="60061" checksum="BDBF8F9D00C68A42" modified="2024-05-29" version="1">MELSARCQSCGERSLVTDEVSENLICTSCAVVQDYNNFQAHVGGISGPAGTYVRTGTSGSGNNYSYKESKIYKAGVLIDDILFKLNFSVSSIDEVKKMIVTITEGEYGQGDWFSIFVGACAYVVRRKNGNLLPMTDVAAVLGRMVNRVVSFLDLKLPEFDIVYLYETTLRNSPSFSCIEAEKKEVMLKQGVFLVQCLIKWFVTTGRRPVQVVVSVLVFIGQLNQLNVKIEDLASELNVPAVTCRLRYKELLERLVEVARVLPWGKDVNIKNIVKFAPYVIQYMEMKSMGNGLKQIKNSDNVGFDIAYFLSDCLQERSDNMIDSYRSCEGDDFFSHNTEQEQSPSLDYANLDKFNISPECLKMIYSKCMEEVSDFRSTSEFGKENRKRRKRHDNYLDCTDWWKGKSELSKKLSLKKILEKDVGMDVMPPAFVNGCLTYQMRRDKIEAAKARIKNIMFPSNAGLHAGYSHCLSTTAKKEKKKKKRHVDVDWEDLIIETLLLHQVKEEEIEKGYYKTLMDLHVFNSGCFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161X1P6</accession>
    <name>A0A161X1P6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0726787</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Required for RNA silencing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RNA polymerase II, IV and V complexes. Interacts with NRPD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161X1P6"/>
    <dbReference type="Gramene" id="KZM86213">
        <property type="protein sequence ID" value="KZM86213"/>
        <property type="gene ID" value="DCAR_023347"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195390"/>
    <dbReference type="OMA" id="DTSMVLF"/>
    <dbReference type="OrthoDB" id="282270at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000419">
        <property type="term" value="C:RNA polymerase V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10508">
        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034012">
        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOH07357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="114" mass="13073" checksum="1254ECB4E2EEC60F" modified="2016-07-06" version="1">MSTMKFCRECNNILYPKEDKEQKVLLYACRNCDHQEAADNNCVYRNEVHHSVGERTQVLQDVAADPTLPRTKSVRCSVCNHGEAVFFQATARGEEGMTLFFVCCNPVCGHRWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161XF28</accession>
    <name>A0A161XF28_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161XF28"/>
    <dbReference type="Gramene" id="KZM91413">
        <property type="protein sequence ID" value="KZM91413"/>
        <property type="gene ID" value="DCAR_021222"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226738"/>
    <dbReference type="OMA" id="TIDCRAR"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="481" mass="52395" checksum="A7B31C2E0AD4E023" modified="2016-07-06" version="1">MENPSPAINILEHCGISPSAEAITVTSLPLSFFDIMWLTFHPLGRVIFYDFPYSTTHFTQNVVPNLKTSLSLALKHFTPLAGNLIIPSNTDSNIDIGIRYMDGDSVSVTFAECTGAVNQFSGNHVRLADIMNPLVAQLPSGTCAEISGESCSVAPLIAVQVTVFPNQAICIGITNSHVVADGNTMFNFVRAWASIAKQLNICEAKPDTNDLIASGCFQIPCYDRSLIKDPYGLGPLFKKMRAAGGAGRMEQLMKQKEDSAPDSSKIKVRATFVITEANIQALKNKVLAKRPTLTHVSSFTAVCAYLWTCFAKTRATVWESAHDLDELQNFSFAMDSRPRLDPPLPASYFGNCLVACLGVQTGRVMIGDEGLAAAAEVLGNAISAKVKNGPMHGADKWMEEFAGIIRGEWYIGIAGSPKMDYYNNVDFGWGKALKFEFVEEPLSLSRCSNSKTDIEVSIILPKIEMDLFSTVFTQGLHNLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161Y9U8</accession>
    <name>A0A161Y9U8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0730234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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                <person name="Ellison S."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SQG0"/>
    <dbReference type="Gramene" id="KZM86478">
        <property type="protein sequence ID" value="KZM86478"/>
        <property type="gene ID" value="DCAR_023612"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194461"/>
    <dbReference type="OMA" id="YQLDVMS"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13844">
        <property type="entry name" value="CuRO_1_BOD_CotA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13868">
        <property type="entry name" value="CuRO_2_CotA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13891">
        <property type="entry name" value="CuRO_3_CotA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000102">
        <property type="entry name" value="Multi-copper oxidase type I family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000081">
        <property type="entry name" value="Spore coat protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000087">
        <property type="entry name" value="Spore coat protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052152">
        <property type="entry name" value="LPR1/LPR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48461">
        <property type="entry name" value="MULTICOPPER OXIDASE LPR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48461:SF1">
        <property type="entry name" value="MULTICOPPER OXIDASE LPR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853213" description="Plastocyanin-like domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="138"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="267"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="412"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KZM86478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH07649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="65061" checksum="F5D6867E182BECA6" modified="2016-07-06" version="1">MERLLSLVTLCLAVFGFCVDKTWGVGLLDPSKLPMFVDELPDMPRLSGFEVVNGVPVSKSLKIGMFRKKWKFHRDLPPTPVFAYGTSAKAATVPGPTIEAIHGVPVNVTWQNHLPRKHILPWDPTIPTAMTMVKTGIPAVVHLHGAIDEPESDGNAYAWFTPKFKERGSTWTKKTYHYSNAQHPGNLWYHDHAMGLTRVNLLAGLIGSYIIRHPAVEEPFRLPHGNQYDRPLVVFDRAFRRDGSLYMNSTGNNPSIHPQWQPEYFGDAIIVNGKAWPRMVVRRRKYRFRILNASNARFFRFYFTNGLKFIHVGSDSTYNQHPVTVNKLLVAPSEIADVVVDFSGSKSNSVVLANDAEYPYPSGDPVNKINSRVMKFLITRKKELDTSRVPAKLVHYPSPDASSASQTRFITMYEYTTKSDEPTHLNLNGKSPDDPVTEQPKVGTSEIWYVINLTDDNHPLHIHLGLFSVLDQTELIKLDEFKECMMKLKDATKCHVDKYARGRNTAVVAHEKGWKNVYKMLPGYVTKILVRFSYVHTNASYPFDATSEPGYVYHCHILDHEDNVMMRPLKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164SX94</accession>
    <name>A0A164SX94_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_023928</name>
        <name evidence="14" type="ORF">DCAR_0727444</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000007">
        <property type="protein sequence ID" value="KZM86794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164SX94"/>
    <dbReference type="STRING" id="79200.A0A164SX94"/>
    <dbReference type="Gramene" id="KZM86794">
        <property type="protein sequence ID" value="KZM86794"/>
        <property type="gene ID" value="DCAR_023928"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108195401"/>
    <dbReference type="OMA" id="ETHWENA"/>
    <dbReference type="OrthoDB" id="1103324at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000019">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943:SF8">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47943">
        <property type="entry name" value="CYTOCHROME P450 93A3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="248"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="EMBL" id="WOH08008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="536" mass="60578" checksum="F9BA9B98EBFBBDD3" modified="2016-07-06" version="1">MANGTDSLYFSPSSILTAGFQDSVVKVCIWLIPALIILRAILKSRTSSRLPPGPPRLPIIGHLHLLAPIPHQALYKLAVKYGPLYHIYLGNNPCVIISSPEMAKEFLKTNELSCSNRPESMASEYLSYHSQDFSYAPYGPYWRFVKKLCMSELLSGQSLDFLHTVRRFEIESMVKEMLQKARAVEAVDVGGVITRITNNVISNMIMSKRCSDTDDEAGEMRKMIKEISKLLGEFNPADYVWFYKFLDLQRIKKRLVDVRARYDRLAQSIIDERREIRKTQEKIAADKDFLDLLLDVYEDEKAEFRLNMDKIKAIILDMFTTGTEAVANVTEWGLAELLNHPMIMDKARQEIDSVVGKSRLVQESDLVNLPYLQAIVKETLRLHAASPLIPRQASEDIVIGNYHIPAKTNVLVHDWAIGRDETHWENALEFIPERFLKTSGENGITGSAGHMDIRGQHYQLLPFGSGRRACPGISLSLKVIQATIAAMIQCFEWNVQGEGNDSIPPVKMDEGRALVLERVHPLICIPVARLSPFPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TXG4</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>Tracheophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
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            <taxon>Apioideae</taxon>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="OS07G0418000 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="WOH09483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="268" mass="30195" checksum="6D249FAB336EE4F9" modified="2016-07-06" version="1">MGDLYALDFDGVICDSCGESSVSAVKAAKVRWPSLFENVSSSLEAWIVDQMHIVRPVVETGYENLLLVRLLVEIQIPSLRKSSVAEGLTVEGILESWSKLKPVIMEEWGEDKDTLVDLFGKVRDEWMDNDLTTWIGANRFYPGVSDALKFASSKVYIVTTKQSRFADALLREIGGITIPPERIYGLGSGPKVEVLKKLQKIPEYQGLTLHFVEDRIATLKNVIKEPELDGWNLYLGDWGYNTQKERDEAASISRIHLLQLSDFSKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiales</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the C-terminally encoded plant signaling peptide (CEP) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH10094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM88647">
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        <property type="gene ID" value="DCAR_025722"/>
    </dbReference>
    <dbReference type="OMA" id="GIACHAI"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:nitrate import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of leaf morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033250">
        <property type="entry name" value="CEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33348">
        <property type="entry name" value="PRECURSOR OF CEP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33348:SF44">
        <property type="entry name" value="PRECURSOR OF CEP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0732">Signal</keyword>
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    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="6"/>
            <end position="25"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH10094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="193" mass="20265" checksum="F271C30E459FA8D8" modified="2016-07-06" version="1">MAQMQAIYSWAILLAVITCHGILSGEGRQLKPMKKEVGVQTVPITKDNITPSTKSMESSTVAEPIADFRPTTPGNSPGVGHSYTEHSVVVQPNEASKISKLLKESKQESTTKQKVPTVSNSIDGFRPTAPGNSPGVGHAFTGNDVKVQTKGVGIGPAIGHSNAGSTDDFKPTQPGHSPGVGHSFRNKNTEPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VKC1</accession>
    <name>A0A164VKC1_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_022159</name>
        <name evidence="2" type="ORF">DCAR_0623993</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VKC1"/>
    <dbReference type="Gramene" id="KZM90476">
        <property type="protein sequence ID" value="KZM90476"/>
        <property type="gene ID" value="DCAR_022159"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZM90476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH04583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="78" mass="8899" checksum="1AD8060A4399EDBA" modified="2016-07-06" version="1">MIWYLVFHGDFSLAIELTNKEAPLCINTTSGLLLKVELAFLKDSTFASKPSIHCKTQNNCRVPNYEQVKIERLKNINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VPQ7</accession>
    <name>A0A164VPQ7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_021971</name>
        <name evidence="4" type="ORF">DCAR_0624200</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I3 (leguminous Kunitz-type inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VPQ7"/>
    <dbReference type="STRING" id="79200.A0A164VPQ7"/>
    <dbReference type="Gramene" id="KZM90664">
        <property type="protein sequence ID" value="KZM90664"/>
        <property type="gene ID" value="DCAR_021971"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192852"/>
    <dbReference type="OMA" id="GANGWRY"/>
    <dbReference type="OrthoDB" id="1387960at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007853956" evidence="2">
        <location>
            <begin position="21"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM90664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH04788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="205" mass="22326" checksum="87D50EBB8153E194" modified="2016-07-06" version="1">MKSNTCILIVLLYGSYLAAAILPPTVYDKSQQKVIAGIRYYILPISRGHGGITIASTRNKTCPLDVAQELSVYNRGLGVTFNRVDASAHTVRVSTDLTIKFSSPAKACAQSSYWKVDGYDNLRARYFVTIGGVGGKHSGKSLPGTFRIEKEGNGYKIVYCRPVNCVATPCEPVCKNVGVYEQHGGILLALADEPLVVKFRKVYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164VPV3</accession>
    <name>A0A164VPV3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_021967</name>
        <name evidence="5" type="ORF">DCAR_0624204</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM90668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164VPV3"/>
    <dbReference type="STRING" id="79200.A0A164VPV3"/>
    <dbReference type="Gramene" id="KZM90668">
        <property type="protein sequence ID" value="KZM90668"/>
        <property type="gene ID" value="DCAR_021967"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108228116"/>
    <dbReference type="OMA" id="CGFKQYG"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000808">
        <property type="entry name" value="Iridoid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF0">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="3">
        <location>
            <begin position="88"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KZM90668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH04792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="388" mass="44189" checksum="C7E75E1A3D6D175B" modified="2016-07-06" version="1">MSWWWSGAVGAARKTLNQDKVPSSYESVALVVGITGIVGNSLAEILPLPDTPGGPWKVYGVARRPRPPWDANHPVEYIQCDISNPEETQSSLSRLNDVTHLFYVTWASRSTEAENCEINSKMFRNVLNCIIPNAPKLQHICLQTGKKHYFGSFEASGIAAHDPPYSEDLPRLESPNFYYNLEDILFEEVKKKDGLTWSVHRPGTIFGFSPHSMMNMICTLSVYAAICKHEGTVLRFPGTKEAWSLYSEASDADLIAEQEIWAAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFEVECGEFEGDERLTLVELMKDKESVWDEIVKEYNLVPTKLEDIGVWWFVDYILGIEYPLDTMNKSKEHGFLGFRNSKSSFIHWIDKLKDSKIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164XHM4</accession>
    <name>A0A164XHM4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_016433</name>
        <name evidence="4" type="ORF">DCAR_0518788</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164XHM4"/>
    <dbReference type="Gramene" id="KZM93188">
        <property type="protein sequence ID" value="KZM93188"/>
        <property type="gene ID" value="DCAR_016433"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108219689"/>
    <dbReference type="OMA" id="AGHNNCE"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM93188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="372" mass="42737" checksum="37604D2C9E62ADE1" modified="2016-07-06" version="1">MGGVTSSMAAKFAFFPPNPPSYKVIKDGFTGLLLLDPFPHRENVDVLKLPTRRGNEIVAMYVRYPMATSTLLYSHGNAADIGQMYELFIELSIHLKVNLMGYDYCGYGRSTGKPSEQNTYADIEAAYKCLKENYGAKQEDIILYGQSVGSGPTLDLAARLPQLRAVVLHSAILSGLRVMYPVKRTYWFDIYKNIDKIPLVTCAILVIHGTADEVVDCSHGRQLWALCREKYEPLWLKGGSHCNLELYPEYLKHLKRFISHVEKTQSRRNGSRNSVDGRLEHSRSTDYVEAPRKSTDRRDKRRQSTSKNEKLKFYEYKFDNDNKFAAKLKIPSDQIEKSRRSVEYHEKSRKSVDKQFERARRSVDWVDRIQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Apiales</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_016802"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EMBL" id="WOG99855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="99" mass="11387" checksum="CC269DF687BC5620" modified="2016-07-06" version="1">MSWRSWRESIENLKSVILIRGARDVSDLTAWIVAGTLVYYLDIVPAQERKQQLKEKAARDACDPNRYIEKCKPVPDPQVTGLIYGNKTRVRSTNKPKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">DCAR_0520494</name>
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    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
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            <taxon>eudicotyledons</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOH01113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_017888"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="203"/>
        </location>
    </feature>
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        <location>
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            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH01113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="400" mass="43908" checksum="90E89AA9AA942C53" modified="2016-07-06" version="1">MPKSKVFSRVKPFLAVLFLQFGLAGMDVISKVALDQMMSTYVFVVYYHAIATILTAPLAIILEKKVRPKMTKAMFGKFILLALLEPVIDQNLYMMGMKNTSATFAAALCNILPAITFVMAWIFGLEKVKLKSIRSQAKIGGTLTTIGGAMLMTFVKGPVIHLMCTEGRTNPVQRSNGVGYQHSLRGSLMITASCFSWACFMVLQANTLETYPAELSLTAWICLMGTVEGAVLALVMERGNAAVWSLNFDAKLLAAVYSGIICSGLAYYVQGIAMKDRGPVFVTAFNPLGMILVAVLGSFILAEQIHLGGVIGAFVIAVGVYLVVWGKSKDYKPRDLSIDNENALKSEQIIDVSRMNDEENHKVSEQMIEASRIYDKDHHEVLAISESMEEEKESDKTLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZD39</accession>
    <name>A0A164ZD39_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_018990</name>
        <name evidence="4" type="ORF">DCAR_0521725</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZD39"/>
    <dbReference type="STRING" id="79200.A0A164ZD39"/>
    <dbReference type="Gramene" id="KZM95748">
        <property type="protein sequence ID" value="KZM95748"/>
        <property type="gene ID" value="DCAR_018990"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108220241"/>
    <dbReference type="OMA" id="ARGNVWA"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01855">
        <property type="entry name" value="YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050896">
        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048422">
        <property type="entry name" value="NOA1/YqeH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21516">
        <property type="entry name" value="YqeH-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="309"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="NOA1/YqeH-like C-terminal" evidence="2">
        <location>
            <begin position="425"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM95748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH02336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="574" mass="63121" checksum="CC205B9FADFC8D27" modified="2016-07-06" version="1">MMLRTLSPSKFKHLIPLINSSYYTYTKPASNILLQPQSIPFLLNPAKTLIPSFSTFLKPFSSQPNFRDGNYDESRSLSVHICPGCGVQMQDLDCKKPGFFVKPSVKGANYKTLIDRNPVAEELEISGSVKKGFLEIENDETPEKNEVKMAKKPVVCSRCHSLRFYGKVKDSSVENLLPDFDFDHTVGRRLSSASGPRSVVLMVVDAVDFDGSFPRTVAKLVSNTIEENYRAWKEGKAGNVPRVLLVVTKIDLLPTSVSPTGLEHWVRSRAREGGASKLAGVHLVSAKRNWGLKNLVDDVVGLAGSRGHVWAVGAQNAGKSTLINAIGKCVGGNVTHLTEAPVPGTTLGILRVEGVLPRQARLFDTPGLLHPHQITTRLTKEEQKLVHISKELKPRTYRIKAGYSIHIAGLMRLDLEETPLDSIYVTVWASPYLPLHMGKTENVLAMLEEHFGHQLQPPNGKSRVEELGNWVKKEFRVSGNSWDFSSVDIAAAGLGWFAISLKGEALLNVWTYEGVNVISRNSLILHRSHDFEVAGFTVSQIVSDADRALNKSSKTLKSKKAGNLTVATATVTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165WV76</accession>
    <name>A0A165WV76_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_014940</name>
        <name evidence="4" type="ORF">DCAR_0415886</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM97698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165WV76"/>
    <dbReference type="Gramene" id="KZM97698">
        <property type="protein sequence ID" value="KZM97698"/>
        <property type="gene ID" value="DCAR_014940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007868833" evidence="2">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM97698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="110" mass="11970" checksum="6799561F651F10F0" modified="2016-07-06" version="1">MRSLGFILVLMLVAIADTNIVTEARNVKLWKTNVVEQDPRQYDHMLPKGPVPPSGPSHGNNPPLERSYGLPDVLPSQHFHIHVLPKGDHHPPPGPSHGSTPDPPGPPPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165XAG2</accession>
    <name>A0A165XAG2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0416241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARR family. Type-A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165XAG2"/>
    <dbReference type="Gramene" id="KZM97975">
        <property type="protein sequence ID" value="KZM97975"/>
        <property type="gene ID" value="DCAR_014663"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217898"/>
    <dbReference type="OMA" id="MAGINDD"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000159">
        <property type="entry name" value="Two-component response regulator ORR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF99">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG96903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="153" mass="16992" checksum="A7A761B0611F23DE" modified="2016-07-06" version="1">MDVSASLVDGGRVEGDVQELHVLAVDDNLIDRKIVEKLLKNFSCKVTTAENGLRALEYLGLRDDHQDTLSGNGSKVNMIITDYCMPGMTGYELLKKIKESSMLKEVPVVIMSSENIPTRINKCLEEGAQMFMLKPLKQSDVKKLRGQLMKCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165YBS0</accession>
    <name>A0A165YBS0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0417329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AG-peptide AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165YBS0"/>
    <dbReference type="Gramene" id="KZM98955">
        <property type="protein sequence ID" value="KZM98955"/>
        <property type="gene ID" value="DCAR_013683"/>
    </dbReference>
    <dbReference type="OMA" id="MEATKMI"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039281">
        <property type="entry name" value="AGP3/12/13/14/21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34114">
        <property type="entry name" value="ARABINOGALACTAN PEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34114:SF11">
        <property type="entry name" value="ARABINOGALACTAN PROTEIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG97988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="56" mass="5563" checksum="987CAF0C987B4249" modified="2016-07-06" version="1">MEATKMIFIAVVMMMAAVQLVAAADAPAPSPASDASVVAVPAALASVLAVVYAFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z0H5</accession>
    <name>A0A165Z0H5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0417906</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z0H5"/>
    <dbReference type="Gramene" id="KZM99465">
        <property type="protein sequence ID" value="KZM99465"/>
        <property type="gene ID" value="DCAR_013173"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215534"/>
    <dbReference type="OMA" id="WILTTRQ"/>
    <dbReference type="OrthoDB" id="2015618at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000019">
        <property type="entry name" value="BZIP family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG98562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="352" mass="39755" checksum="36B012F3F68858E9" modified="2016-07-06" version="1">MASPSTQFATTRRMGMYDPLHHISTWEDILGAESSVNITSSCTILQADTRLLNKSYTSQESLGELSDNQAGRTKCDKLQRRLAQNREAARKSRLRKKAYVQQLETSRLKLAQLELEIDRTKQGLYTGGAGHSGTINSGIISFEIEYGLWVELQEKKNCQLRNVLQAQISEKELRVHVEGGLAHFSDLFRMKAEAAKADVFHVMHGMYRSPLERLFLWIGGFKPSEVIQILVPQLEPLTEQQLVDVYKLRHSCQQAEDALSQGMEKLVQTLAQCVVLDNIAGGSYGSRMASAMERLESLECFMNQADHLRQQTLQQMSRILTTSQAARGLLAFGEYFQRLRALSSLWAVHPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z107</accession>
    <name>A0A165Z107_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoenolpyruvate carboxylase</fullName>
            <ecNumber evidence="5">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0417929</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxaloacetate + phosphate = phosphoenolpyruvate + hydrogencarbonate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PEPCase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z107"/>
    <dbReference type="Gramene" id="KZM99485">
        <property type="protein sequence ID" value="KZM99485"/>
        <property type="gene ID" value="DCAR_013153"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217635"/>
    <dbReference type="OrthoDB" id="1365747at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.90:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.90">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00595">
        <property type="entry name" value="PEPcase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022805">
        <property type="entry name" value="PEP_COase_bac/pln-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018129">
        <property type="entry name" value="PEP_COase_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033129">
        <property type="entry name" value="PEPCASE_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000584">
        <property type="entry name" value="PRK00009.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523:SF33">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00781">
        <property type="entry name" value="PEPCASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00393">
        <property type="entry name" value="PEPCASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG98585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="959" mass="109683" checksum="13880708062E8450" modified="2016-07-06" version="1">MADRNVEKLASIDAHLRSLVPGKVSEDDKLVEYDALLLDRFLDILQFLHGEDLRETVQECYELSADYEGQHNPQKLEELGKVLTSLDPGDSIVVAKSFSHMLNLANLAEEVQIANRRRNKLKKGDFVDESSAITESDMEETFKKLVQMNKSPEQIFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLAQLYTEDITPDDKQELDEALQREIQAAFRTDEIRRAPPTPQDEMRAGMSYFHETIWKGVPQFLRRVDTALKTIGIKERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFEMSMWRCSDEARLRADEIHTTAKRDTTKHYIEFWRQVPTSEPYRVILGEVRDKLYYTRERSRHLLSHGKSDIPEEATYTKIDQFLGSLELCYRSLCACGDQLIADGSLLDFLRRVSTFGLSMVRLDIRQESERHTDVLDAITRHLEIGSYREWSEEKRQEWLLSELRQKRPLFGPDLPRTEEIAEVLDTLHIISELPSDCFGAYIISMATSPSDVLAVELLQRECHVMEPLRVVPLFEKLADLEAAPAAIARLFSIEWYKNRIDGKQEVMIGYSDSGKDAGRFSAAWQLYKAQEELVKVAKQYGVKLTMFHGRGGTVGRGGGPSHLAILSQPPETIQGSLRVTVQGEVIEQSFGEQNLCFRTLQRFTAATLEHGMHPPISPKPEWRQLMDEMAAVATEEYRNIVFKEPRFVEYFRLATPELEYGRMNIGSRPSKRKASGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAFKHVIEAKQENLQMVQEMYNTWPFFRVTIDLVEMVFAKGDPGIAALNDKLLVSEDLWPFGEQLRSKYQETQSLLLQIAGHKEILEAAPTLKQRIRLRDSYITTLNVCQTYTLKRIRDPSFSVTCRPHITKEKFASLVDLNPTSEYAPGLEDTLILTMKGIAAGMQNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A165Z7F6</accession>
    <name>A0A165Z7F6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_012932</name>
        <name evidence="4" type="ORF">DCAR_0418171</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000004">
        <property type="protein sequence ID" value="KZM99706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG98826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A165Z7F6"/>
    <dbReference type="Gramene" id="KZM99706">
        <property type="protein sequence ID" value="KZM99706"/>
        <property type="gene ID" value="DCAR_012932"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108217528"/>
    <dbReference type="OMA" id="CLARIEY"/>
    <dbReference type="OrthoDB" id="591557at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006527">
        <property type="entry name" value="F-box-assoc_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="F-box associated beta-propeller type 1" evidence="2">
        <location>
            <begin position="91"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZM99706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG98826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="373" mass="42884" checksum="89246CA62072F788" modified="2016-07-06" version="1">MSPSLPEHIILEEILTRLPAASLARFKFVSKSWMALFSHPNFVKSHINRKEDYLIRTCPGGIGILSRTTLTESHIEDVPASFDGLVGSVTGLVCGVSESRNCFILWNPILHVYKQIPLPKHSKYSLFGFCWDSATEDFKLVAKKFDSSSAVVYSTKTNRWINIRAFRIPYAGDEFPAVIVKGNPYWTGYFRSPGILKFEARTNRFTCLGLTCVAGKRYSLCSVNDCLARIEYSLTRGNCLELYQFNDGRGVWSKMYTINIKTTYIFTIPKCFNYSGEIVFSGCHEWCDTKLNEVESLEYEFGYELGPSRLHRYSYTPSLIILDGMKTSTRHLRDKHRKLRSVWCMRLPRKYRRSRHFSVYDISLPTYCGGNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166A623</accession>
    <name>A0A166A623_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Catalase</fullName>
            <ecNumber evidence="4 15">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0310778</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="4 15"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166A623"/>
    <dbReference type="SMR" id="A0A166A623"/>
    <dbReference type="Gramene" id="KZN00782">
        <property type="protein sequence ID" value="KZN00782"/>
        <property type="gene ID" value="DCAR_009536"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214439"/>
    <dbReference type="OMA" id="QVTWLFG"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08154">
        <property type="entry name" value="catalase_clade_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000002">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF23">
        <property type="entry name" value="CATALASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 15" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 15" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="13">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG91529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="492" mass="56593" checksum="2945C118E059B7FF" modified="2016-07-06" version="1">MDPYKHRPSSAFNSPYWTTNSGAPVYNNNASLTVGTRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISQLTCADFLRAPGVQTPVIVRFSTVIHERGSPETIRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLNMFTFLLDDIGIPQDYRHMDGSGVNTYTLINKAGKAHYVKFHWKPTCGVKSLLEDEAIKIGGANHSHATQDLYDSIAAGNYPEWKLFIQIIDPDHEDKFDFDPLDVTKIWPEDILPLMPVGRLVLNKNIDNFFAENEQLAFCPAIIVPGVYYSDDKLLQTRIFSYADTQRHRLGPNYLQLPANAPKAAHHNNHHDGLMNFMHRDEEVNYFPSRFDPVRHAERHPIPPPMLTGKRDRCMIEKENNFKQPGERYRSFCSGRQERFVNRLVDALSDPRVTHEIRSIWISYWSQADKSLGQKLASRLNVRPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166DXD7</accession>
    <name>A0A166DXD7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sucrose-phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0207472</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the final step of sucrose synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>sucrose 6(F)-phosphate + H2O = sucrose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="EC" id="3.1.3.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Glycan biosynthesis; sucrose biosynthesis; sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the sucrose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.24" evidence="9"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166DXD7"/>
    <dbReference type="Gramene" id="KZN05797">
        <property type="protein sequence ID" value="KZN05797"/>
        <property type="gene ID" value="DCAR_006634"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206327"/>
    <dbReference type="OMA" id="VENCDAY"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050307">
        <property type="term" value="F:sucrose-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02605">
        <property type="entry name" value="HAD_SPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013679">
        <property type="entry name" value="SPP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051518">
        <property type="entry name" value="Sucrose_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012847">
        <property type="entry name" value="Sucrose_phosphatase_pln/cyn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01485">
        <property type="entry name" value="SPP_plant-cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521:SF8">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08472">
        <property type="entry name" value="S6PP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01141">
        <property type="entry name" value="C2.B.1:_Sucrose_Phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose-phosphatase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG88238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="426" mass="48525" checksum="0FD5579FE84AC01C" modified="2016-07-06" version="1">MDRLNGPARLMIVSDLDQTMVDHNDRENVSLLRFNALWEAEYRQDSLLVFSSGRSFASYEQLCAKKPMLTPDITVMSVGTEIAYGESMVPDNEWKQSLDKNWNRDIVVEETSKFPELTPQWEQSQRPHKVSFFLEKDKALKVINALTERLEVRGLDAKTIYSGGEALDVLPKGAGKGQALSYLLKKFEVNGNRPNNTLVCGDSGNDAELFSVPEVYGVMVSNAMEELLQWREENAKNNPKILLATERGAAGIIQAIGNFGLGSSISPRDRVDLSEFGSKTLCPDHEIVMFYLFYERWRRAEVENTEQSLHKLRSICHSMAIFVHPSGVKRPLQHSIDEMVKIHGDKKGKRFSVWVDQVYSSEIALNTWLVKFYKWELCGEEKEKYCCLTTVLLSSKTEESDGLMWLHLHQTWLEGQGTEESSTWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H1U0</accession>
    <name>A0A166H1U0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_002275</name>
        <name evidence="13" type="ORF">DCAR_0102358</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Channel protein in tonoplast. These proteins may allow the diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H1U0"/>
    <dbReference type="SMR" id="A0A166H1U0"/>
    <dbReference type="STRING" id="79200.A0A166H1U0"/>
    <dbReference type="Gramene" id="KZN09619">
        <property type="protein sequence ID" value="KZN09619"/>
        <property type="gene ID" value="DCAR_002275"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108194274"/>
    <dbReference type="OMA" id="HINPAMS"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF37">
        <property type="entry name" value="AQUAPORIN TIP2-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KZN09619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG83184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="249" mass="24799" checksum="BBF0A2E32B9DE04D" modified="2016-07-06" version="1">MVKISIGSLGDSLSHGSLKSYLAEFIATLLFVFAGVGSAIAFNNLTADAGLDPAGLVAIAVAHAFALFVGVSMAANISGGHLNPAVTFGLAVGGNITLITGFFYWIAQLLGATVASFLLIFVTGGKAVPIHGVGAGLGAAEGVVFEIIITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVAAGDFSGHWIYWVGPLIGGGLAGLVFGDVFIGSYAAVPASDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166H627</accession>
    <name>A0A166H627_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0102523</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166H627"/>
    <dbReference type="Gramene" id="KZN09770">
        <property type="protein sequence ID" value="KZN09770"/>
        <property type="gene ID" value="DCAR_002426"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226306"/>
    <dbReference type="OMA" id="FTGWYVY"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF50">
        <property type="entry name" value="PROTEIN LIFEGUARD 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG83348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="244" mass="27046" checksum="DD5F06D59518B286" modified="2016-07-06" version="1">MYGFTSISSKEDVDIESGSSTLYPGLSHGENQLRWGFIRKVYGILAAQMILTTIISSVTVLYAPVNEVLRGNSGLLLFLLVLPLVLLWPLYVYQQKHPINLVLLGLWTSCLSITVGVSCANTDGKIVLEALILTSAVVASLTAYTFWASKKGKDFSFLGPILFTSLFVLLLTSFLQMFFPLGSTSTAVYGGISAVVFSGYIVYDTQNLIKRFSYDEYIWATVTLYLDILNLFLTILRMLRQGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166HTJ9</accession>
    <name>A0A166HTJ9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S acidic ribosomal protein P3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_003035</name>
        <name evidence="3" type="ORF">DCAR_0103198</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN10379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166HTJ9"/>
    <dbReference type="STRING" id="79200.A0A166HTJ9"/>
    <dbReference type="Gramene" id="KZN10379">
        <property type="protein sequence ID" value="KZN10379"/>
        <property type="gene ID" value="DCAR_003035"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108206521"/>
    <dbReference type="OMA" id="EWNAKQL"/>
    <dbReference type="OrthoDB" id="2015129at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044252">
        <property type="entry name" value="RPP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47207">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P3-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47207:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P3Y-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZN10379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG84019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="12239" checksum="E8091A3A37EC8139" modified="2016-07-06" version="1">MGVYTFVCRSSGGEWTGKQHKEGDLEASAASTYELQRKLVQTVLSTDSSGGVQSSFSFVCPNSAVFQVIVGGAALVGGGGVAAAASGGAAPAAEAAAPAEEKKAEKEESEDEDLGFSLFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IZC8</accession>
    <name>A0A166IZC8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IZC8"/>
    <dbReference type="Gramene" id="KZN11654">
        <property type="protein sequence ID" value="KZN11654"/>
        <property type="gene ID" value="DCAR_004310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006527">
        <property type="entry name" value="F-box-assoc_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="185" mass="20865" checksum="086BC41F9B32BFFD" modified="2016-07-06" version="1">MVIVYSLKTNSWKHILNVPAHTTRFFGDWGMSASGALHWLAIDNPINGSHIIVGFDLGLEQFKKLPAPALEGPINTRCVVSVGSSLWILDEYPDSHRDMWVMLNSSGGEISWSKILSKRSALTYLRSVRPVYISMSDPGILFEVDSSQLVWYDLERKMLKNVRIRGPTDKFDHIVHSESHTAQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J1N5</accession>
    <name>A0A166J1N5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_004373</name>
        <name evidence="4" type="ORF">DCAR_0104721</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN11717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J1N5"/>
    <dbReference type="STRING" id="79200.A0A166J1N5"/>
    <dbReference type="Gramene" id="KZN11717">
        <property type="protein sequence ID" value="KZN11717"/>
        <property type="gene ID" value="DCAR_004373"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108212000"/>
    <dbReference type="OMA" id="HYWAHPS"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF52">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007875579" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KZN11717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG85531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="311" mass="35260" checksum="176F025CCBA7781F" modified="2016-07-06" version="1">MAISLVTLFGGIIRRTLSAASLSSQTIAIDDETTIHFWSPKPTTTSSSTAKPPLLLIHGFGPHGVWQWQQQINFFAKSYSLYIPNLVFFGGSYSTSSERSEIFQARAMGKLMEKVGVEKYYVVGTSYGGFVAFRMAEMWQDRVMKVVIANSAVNGLLSDSMELVKRGKVEKIEDLIMPEKASELRKLMNLSVFRRAYVPDFILNDFINRLYSKNRKEKLELLRGLTIGWDDIMKASPLQQEVLIVWGEHDKIFLLDLAMELKKVLGKNVKLKVIKNASHIPQVENAWKFNNIIDTFLRDSPDQGLSLMSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J2F5</accession>
    <name>A0A166J2F5_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104725</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. Plant myosin class XI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Gramene" id="KZN11720">
        <property type="protein sequence ID" value="KZN11720"/>
        <property type="gene ID" value="DCAR_004376"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108203481"/>
    <dbReference type="OMA" id="LGHPRSF"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15475">
        <property type="entry name" value="MyosinXI_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01384">
        <property type="entry name" value="MYSc_Myo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000001">
        <property type="entry name" value="unconventional myosin-Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WCA1</accession>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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</entry>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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                <person name="Zheng Z."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="gene ID" value="DCAR_031981"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG86502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="65" mass="6930" checksum="B39C5A044C1D320D" modified="2016-09-07" version="1">MASKSSMESLNSTGRLNSASCISSHLSRLVLAAVEGDGDREENVPEVPVENDFLLNGELDGEDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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    <name>A0A175YC94_DAUCS</name>
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        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS4c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rps4</name>
        <name evidence="15" type="ORF">DCAR_0100123</name>
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        <name evidence="16" type="ORF">DCAR_0C036276</name>
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    <organism evidence="14">
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Moranska E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </lineage>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the EAF1 family.</text>
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    </dbReference>
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        <property type="gene ID" value="DCAR_029722"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000416">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="319"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH14772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="644" mass="73656" checksum="3CF28682CDDDC2A6" modified="2016-09-07" version="1">MAPKSSIRLISYSEELIDGRPLYVSSNCLPVKALYYEPAGHSYHLAAQKLLGFFEKKDVEGDNQDVPNDKVNESIHSSGSYSSSKGKKKSGSGGDQQDHYALLGLSHLRYLATEDQIKKSYRETALKYHPDKQAALILSEVTEAAKQAKKDEIENHFKSIQEAYEALIDPVKRRIYDSTDEFDDEIPTDCAPQDFFKVYGPAFMRNGRWSVNQPVPPLGDDSSSLKEVDDFYNFWYAFKSWREFPHADEFDLEQAESRDHKRWMERQNAKLSEKARKEEYARVRILVDNAYKRDPRIVRRKEQEKAEKQRRKEAKYLAKKMQEEEAARIVEEEKRKKEEEQKRAAEVALQQKKLKEKEKKLLRKEKARLRTLSGSVLSQHLLDLCEDDVEKLCTSLDIEQLKILCNNIEGEEGLGRAELIREALACNNGEKQDGMKQPMQNGHATKVASKIPLGNYEKKEKPWSKEEIELLRKGMQKFPKGTSRRWEVVSDYIGTERSVEEILKATKTVLLQKPDSSKAFDSFLEKRKPAVSIASPLTTREEVVGESLTNGVKETAVKTEDLKESSSKPESPQKSDDVDAASSDQDVWSAVQERALVQALKTFPKETTQRWERVATAVPGKTVNQCKKKFASLKESFRSKKSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YKZ8</accession>
    <name>A0A175YKZ8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0830538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YKZ8"/>
    <dbReference type="Gramene" id="KZM83798">
        <property type="protein sequence ID" value="KZM83798"/>
        <property type="gene ID" value="DCAR_028780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH11059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="24211" checksum="195598D8DD4E3F3D" modified="2016-09-07" version="1">MSTLPQEIIIEEILKRLPVKSLVKLQVVWEVAVYSCKTDCWSYKPVDSDVFRSDIFQFPSVVVKGVPYWKDCYGTKIVKFDVRTNRFTSFINSTNPLGPYYTLVNIYDSLARIRYSYFTEVHLYDEERGVWSKMYGLKNVITRSIMTPMCFKYGGEIVYPDKDNVNCYDPKSDEIKVLLHCKKEYTQGFSYTPSLLSLDGMSSASHWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W8I7</accession>
    <name>A0AAF0W8I7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0104346</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W8I7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF73">
        <property type="entry name" value="OS01G0201200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG85159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="351" mass="39778" checksum="CF00C3BE3710D789" modified="2024-05-29" version="1">MKFYDKSKSTKESAWAKFFDHGQGKVTSVESADEWMLDLSKLFIGLRFAHGAHSQLYHGSYKDEPVAVKMIRVPDDIYDGSLGASLEKQFTREVTLLSRLHHQNVIKFIGACRKPPIFCIVTEYLSEGSLRAYLHKLEENLPLQKLISLALDIGRGMEFVHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTGTYRWMAPEMIKRKSYSRKVDVYGFGLILWEMVSGSIPYEDMTPIQAAFAVVNKNLRPAIPDTCPAAMKALIEHCWSSHPDRRPEFSQIVKILEQFEASLASDGNLDSVQNLTYHDHKKGILHLIHKLGPVHHSHHSPPPTPKPRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="7">ENTH domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>campanulids</taxon>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="8">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
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        <text evidence="3">Belongs to the epsin family.</text>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
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                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
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        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG88804.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11303" checksum="9D4BC8FA4B80E4AF" modified="2024-05-29" version="1">MGNGGGLGFFRALFAILILLGFVLSLAGGMIESSVKSETNWINLSESEAGGLKQMKVIDHTLAKKLQVPGPNSDFNFNMNKRRVPNGPDPIHNRKAGNSGRPPGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WFV0"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047574">
        <property type="entry name" value="AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039683">
        <property type="entry name" value="Lsm12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019181">
        <property type="entry name" value="LSM12_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048478">
        <property type="entry name" value="LSM12_LSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13542">
        <property type="entry name" value="LSM12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09793">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21166">
        <property type="entry name" value="LSM12_LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00995">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52001">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AD" evidence="1">
        <location>
            <begin position="90"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG88782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="189" mass="20841" checksum="4ECBFBA5677F83B0" modified="2024-05-29" version="1">MNMTTGVGEEEIAVGCILAIKTTLGDEFEGQVLTFDRVSNILVLQDSVPAAGVLKSSNGRRNIRMLKANFIKEVTFLGQADEDPLDVNKCFLDLNTLHSKELSAIRQAEIDSERIGVGVTPEAQSIFDALSKTLPVRWDKTVIVVMNEVRVSSPYTSERVTGGTPAANERVRKVLELERKRLQNRSPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WG59</accession>
    <name>A0AAF0WG59_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0207774</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WG59"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000130">
        <property type="entry name" value="synaptotagmin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF190">
        <property type="entry name" value="C2 CALCIUM_LIPID-BINDING ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="16">
        <location>
            <begin position="66"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="247"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="473"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG88539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="54664" checksum="FB50BC3E4E80A55B" modified="2024-05-29" version="1">MGLISGILMGMITGIALMAGWSRMMKYRSNKRIAKAVDIKLLGSLSRDDLKKICGENFPEWISFPVYEQVKWLNKQLSKLWPFVADAATAIIKESVEPLLEEYRPAGITSMKFSKLSLGNVPPKIEGIRVQSLKEGQITMDIDFRWGGDPSIILGIEAAMVASIPIQLKDLQVFTVIRVIFQLTDEIPCISAVVVALLSEPKPRIDYTLKAIGGSLTAIPGLSDMIDDTVNTIVTDMLQWPHRIVVPIGGVPVDTSDLELKPQGKLTLTVVKANELKNMEMIGKSDPYVVLYIRPLFKVKTKVVDNNLNPVWDETFELIAEDKETQALIFEVFDQDIGQDKRLGIAKLPLRELEADTTKEMQLRLMPSLDMLKIKDKKDRGTITVKVLYHEFNKEEQYQALEDEKRILEERKKLKEAGVIGSTMDAVGAVGSGIGAGVGMVGTGLGAGVGIVGSGFGAVGSGLSKAGKFMGRSITGQSTSKKSGTSTPVNSTQENGGAKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WHR9</accession>
    <name>A0AAF0WHR9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0207828</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG88593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WHR9"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01989">
        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF100">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="523"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="14">
        <location>
            <begin position="810"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="397"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="224"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOG88593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="881" mass="98960" checksum="2D7191FD586B388D" modified="2024-05-29" version="1">MTLTSLYHAVSHSMIRVPDIALKGLMDSRGDIVEEIAVGEEEMVYVSVSKNVKESESTLKWAVRNFHGKKLCILHVHQPSKKKPSMGTKYSFSRLKDHQARTYHDTERQHMQSVLETYGLICERAGVWAETLHIEMDSIEKGIVELISMNNIRRLVMGAAADKLYSKKMIELKSKKAIYVCQKAPMSCHIWFVCNGNLIYTRRGDLDKASGKVFSPPPPLPSNCHVQSSSPRQSSGQEWRCSELAISASQFPKVRSAISSQDASGGTTPSSSLNNERNLYAWDNISRSDSADSFVSPRSSIEVVENSSLLKFSRPERSRNGIEFRAILNCDEGLYNSSPPSAQEEIASDKLYDELEKTMAEAENSRQKAFEETIKCRKAEKRATEAILRAKASESSFTEELKQKKELEEALASGKEEIEKLKPKLDEIMKELQFALEQKSSLEFQIRDSNETVQELEQKIFSAVHLLQRYKKERDELLVERDNAVGLAEGLKKNPVEDTSIARVTQFYTEFSLSEIEAATHGFNPSLKIGEGGYGNIYAGILRHTQVAVKVPHSNSLQGPLEFEQEVNVLSTLRHPNLVTLIGACSEARILVYEYLPNGSLEDRLNCKENTPPLSWQTRVRIAAELCSVLVFLHSCKPRSIIHGDLKPSNILLDTNHVSKLSDFGTCSLISRDELSSNNTTICCRPDYAKGTFPYIDPEYLSTGELTRKSDVYSFGIILLRLLTGRPALGIAKDVQSALDKGNLKDLLDCTAGDWPFVRAKQLAQLALSCCEMSRNKRPELISEVWRMLEPMKFSCGASSFKRGSEDHLQIPHFYMCPIFQEIMQDPVVAADGYTYETEAIKGWLESGNDKSPMTDRKLANDILVPNHALRSAIQEWLQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WK09</accession>
    <name>A0AAF0WK09_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0310589</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WK09"/>
    <dbReference type="KEGG" id="dcr:108210556"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000010">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002130">
        <property type="entry name" value="DNA mismatch repair protein MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="12">
        <location>
            <begin position="883"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1022"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="WOG91340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1103" mass="121414" checksum="2752CF3F12DDC75E" modified="2024-05-29" version="1">MGKQKQQVISRFFAPNPKTPSNPTPSSPPPPPKISTTVNFSPAKRALNSQFISSTKPPKTPKLSPHTHNPIPSLHQRFVEKLLEPSQDLLEPSGESSLVGPSVKYTPLEQQVVELKRKYPDVLLMIEVGYKFRFFGQDAENAARVLGIYAHMDHNFLTASVPTFRLNVYVRRLVGAGYKVGVVKQTETAAIKAHGSNRLGPFCRGLSALYTKATLEAAEDVGGGEEGFGSCSNYLVCVVENVDIGGIESGCDSKIGIIAVEISTGDVIYGEFDDNFMRSALEALLLRLSPAELLLAEPLSKQTEKLLSSYAGPTSNVRVEHISRDSFNQGSALAEVMALSDNKSDSQLVKNLHKETEVETEAADCSTNEGIVDMPDMVVQALALTIRHLKQFGFEKILCSGASFRPLSGSMEMTLSANAMQQLEILRNNIDGSESGSLLQCMNNTLTISGSRILRHWVTHPLCDRNMIHSRLDAVSEIAESMGSYKASQSINCDEEDSNVTIVCPKLHQVLSSVLTFLGRSPDIQRGITRIFHRTATTSEFIAIIKAILVAGKQLHELHVEEEGRNTIVGKNIVHSVLLRKLVLTASSSRLIGIAAKLLSSLNKDAAEKQDLQNLFISTDGQFSEVGEARTKVQCAKEKLDLLINSYRKQLGKRNLEFTSVLGTTHLIELPADVKVPSNWVKVNSTKKTIRYHPPEVSTALDQLSLVTEELTHICRAAWDNFLKEFGRYYSEFQAAVEALASLDCLHSLAVLSRNKGYVRPVFVNDNDPVQISVRSGRHPVLESILQDSFVPNDTNMHSDGEYCQIITGPNMGGKSCYIRQVALIAIMAQVGSFVPACSASLHVLDGIYTRMGSSDSIQQGRSTFLEELSEASNILHSCSPRSLVIIDELGRGTSTHDGVAIAYATLQYLLEEKRCMTLFVTHYPKIVDIKTQFPRAVGAYHVSYLTSKKDPSTDRNNDQRVDTMNYEDVLYLYKLVPGVSKSSFGFKVAQLAQLPASCISQAISMAVKLEAAVSNRRKNRLACNHSNESASPDNEAKHAPKRLTEHIEDTCAEETEDLEELCTVFREIFLNINLASSVDGDLEKCFQLLNHARGLALELINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKP5</accession>
    <name>A0AAF0WKP5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucan endo-1,3-beta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">DCAR_0310080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKP5"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG90834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31442" checksum="118C88EEF77051C0" modified="2024-05-29" version="1">MRLYDPVQSALQALGGSNIELMLDVPNPQLQSLQTAAGASTWVRNNIQNYLPGVNFRYIAVGNEVDPNNGGTSEYVNLVLPAMRNIHNAIVAAGLQNQIKVSTATYSGLTSGFPPSQGAFRDNVQGFIVPIIQFLAQNNLPLLANIYPYFSYLGTPQGDLQYALFTAPNVVVTDPVGNRQYRNLFDALLDTLYSAVEKSGGPNIEIVVSESGWPSSGGTEASVQNAGTYYRNLINHVKGNGTPKRQGRPIETYLFAMYDENLKGGAETEKHFGLFNPTNQQPKYQLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WKQ3</accession>
    <name>A0AAF0WKQ3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium load-activated calcium channel</fullName>
            <shortName evidence="14">CLAC channel</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0310937</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium-selective channel required to prevent calcium stores from overfilling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TMCO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WKQ3"/>
    <dbReference type="KEGG" id="dcr:108213195"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023322">
        <property type="entry name" value="DUF841_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 14" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOG91687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="183" mass="20532" checksum="95CBF7C4D92E958C" modified="2024-05-29" version="1">MASNLIFTQSKYSDSLTIVAISIFTAIICESISYLLIYRTNSYKSLKSTIDKASKKLETLKKSKTKKIDRFETNLKESNRDLSMFKFKSGAVVALILFVVFKFLSNLFEGKAVAKLPFVPFKIVQKMSHRGLEGEDFTDCAMAFLYFLCSISIRTNLQKFLGFSPPRGAAAAGLFAMPDVKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WMU2</accession>
    <name>A0AAF0WMU2_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0311489</name>
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    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
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        <property type="protein sequence ID" value="WOG92226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WMU2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
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    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOG92226.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="97" mass="11031" checksum="49176BBE521B8A25" modified="2024-05-29" version="1">MALGLILGIGRAFRRKRASSLDILSSKRGPRDYYKGKNCKPTGFHTRKGGYVVVQEKLPNYVVPDLTDFKVNKLNSLINQSPIILNHRDNLRQFNVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAF0WPZ8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="4">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0312935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Gunneridae</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
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        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
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        <property type="entry name" value="IPPc"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="260"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG93649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="577" mass="66280" checksum="081BA0EB12134256" modified="2024-05-29" version="1">MTKTPIWQKSHFYKIFNNFREMNRDSVTSAEETFFIKNKALPRLRRRNSETFRTEYITAREVRIFAGTWNVACQLPTDDLNIEEWLDINEAADIYVIGLQEIISLNAGNIFGVEDIRPIPKWERLIRETLNKIQPVKMKGKCYSDPPSPSKFEQSEDFSPTGDEASVESDGDEQEEIFMSKKESDFKNNYQVIRRDELLPTAGANLSILLDEQLSTHFYAPKTSKAFGDRVKKRVSKKEIVGLSWAEPPLDLLAHCNIDRTTSLTNSKSFKAFNSINFLKSSMNDDTSPKGQPPDTAASTSKVDLEPLVNWKRRSHYVRIVSKQMVGVFITIWVRRSLRRHIQNVNVSTVGVGAMGYMGNKGSISVSMSIYQTLFCFICTHLSAGEKKVDAIRRNADVRAIHSRTHFNSFTTIPLPKSIYDHERIIWLGDLNYRINLPYNETRKLISTSDWTKLIEHDQLVQEFKKGGTFDGWSEGTLNFPPTYKYELNSDKYRGEDSKEGRRTPAWCDRVISFGKGIKLISYGRTELRLSDHQPVAASYMVEVEVFCPKRLQKALKYTGAEIEYELNLLDYELGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WS02</accession>
    <name>A0AAF0WS02_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">Sieve element occlusion N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0312742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
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            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WS02"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010088">
        <property type="term" value="P:phloem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027944">
        <property type="entry name" value="SEO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027942">
        <property type="entry name" value="SEO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039299">
        <property type="entry name" value="SEOA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232:SF9">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14577">
        <property type="entry name" value="SEO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14576">
        <property type="entry name" value="SEO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sieve element occlusion N-terminal" evidence="1">
        <location>
            <begin position="38"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Sieve element occlusion C-terminal" evidence="2">
        <location>
            <begin position="563"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG93458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="92248" checksum="5A6C7BD3B82313EB" modified="2024-05-29" version="1">MTSTMPPVGNALALRHPMNSMFNQQNIIKGDRNMFMSSDDNVMMRQIYDTHTPDGRVLYVKPLLHIVEDILSFTHMTSPLDAANAHGAHMDDKTHQSEVISMLEALSHLIDRISCEISIKCLSGSDGHVTTVSILNMVSNYPWDAKLVLTLAAFAFSYGEFWLLAQLYSSNQLARSMAILKQVPLIMEHSGSLQHRFDAVNTLLNSIMDLTRTIIALEELPTMYIAKDVPPFSSAIETIPTAVYWSIRSVIACATTISLLTSMGHEYTISMTELSWELSTFAQRIKQIHDHLKRNLDICYLHIDEKMRAAAYESLRHLFDVTHIDNMKVLRALIYPRDDIQPLVEGSSKRRVHLDVLRRKNVLLLISGLDISADELSILEQIYSESRIHGTRMESLYELVWIPIVDLSHVTHPGTTPGIYGTTTGPYGTTTGPYGTTTDTFPLQKHTGNLPEDHSLHSSNSKTMSVFSSKNLMDPQNYSAVFKQHWTDPMQKRFDELTSTMPWYSVYHPSIIDQAVVRFVKERWHFRKKPILVVLDPQGKEVSPNALHMMWIWGSTAFPFTSSREEALWRDETWRLELLVSGMDPIIYNDWIRDGKFIILYGGEDIDWIRRFTNTARAVAQAAGVQLEMAYVGKSTKREMVRKAMSTITVEKLSYCWQDPTMIWFFWARMESMLFSKIQVKKRDDHLDPLMQEIKKLLSYDKSGSWAMLCKGSYIVTSGHAQTVLSTLLEFDTWKEYVPLRGFDVAFKEYHDKLHVANPPCCRFEFLTTAGRIPDGMTCPECLQHMEKYSSFLCCHPENAAYLPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WT56</accession>
    <name>A0AAF0WT56_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_0313379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the production of high-mannose type N-glycans during plant development and fruit maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(4)-(oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc)-L-asparaginyl-[protein] + H2O = an oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-D-GlcNAc + N(4)-(N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:73067"/>
            <dbReference type="Rhea" id="RHEA-COMP:12603"/>
            <dbReference type="Rhea" id="RHEA-COMP:18176"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:132248"/>
            <dbReference type="ChEBI" id="CHEBI:192714"/>
            <dbReference type="ChEBI" id="CHEBI:192715"/>
            <dbReference type="EC" id="3.2.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 85 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WT56"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06547">
        <property type="entry name" value="GH85_ENGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000043">
        <property type="entry name" value="cytosolic endo-beta-N-acetylglucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057882">
        <property type="entry name" value="ENGase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25529">
        <property type="entry name" value="Ig_ENGASE1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="9">
        <location>
            <begin position="78"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase C-terminal" evidence="10">
        <location>
            <begin position="561"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="WOG94088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="680" mass="76432" checksum="1066C4670E5DC376" modified="2024-05-29" version="1">MSTPTPTPPPFDPSRPSSPISYPIKTLEELESRSYFDSFHYPFNKSWGCTQGVRSNAAEKSLPMRPRLLVCHDMAGGYGDDKWVQGGDNPDAYAIWHWYLMDVFVYFSHSLVTLPPPCWVNAAHLHGVKVLGTFITEWDEGKAITRKLLETKESAHMYAERLTELAVSLGFDGWLINMEVEVDIEQIPNLKEFVSHLTYAMHSSKDGSLVIWYDSVTVFGKLEWQDHLNDSNKPFFDACDGIFVNYTWKANYPKLSADVAGSRQFDVYMGIDVFGRGTFGGGQWTTNVALDVIKKDNVSAAIFAPGWVLETNQPPDFQTAQNRWWGLVENSWGVSQKYPKSLPFYSNFDQGHGYHIFMDGRKISTKPWNNISCQSFQPLLDCLEDQRPNAIQVSIDFNEASYIGGGNITLKGSLDGDTYFTTRIFQGELLFGNLPVLFTFSVKSDVSSMVGLSLKFSSQALEKTSILLASRGDSVLNTKQFSSKFNRVIMPHQVKRLDATPGWVIQESRLAMEGYTLTGIHIICYRSKHISVNHEIVTQSPSIYNAVLGHIDIRTSATNSDFPPDASWLVEGQFIKWTSGSEASKTVSVKIIWKLKDGTASWFPSYNIYVEKQGIVRNSNALLEERLEYLGLARVEAFYISDLMVPSGISRLNFIIQVCSLDGTLQKLDCSPSFQLNVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WXR4</accession>
    <name>A0AAF0WXR4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoplasmin homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glucose-regulated protein 94 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0417108</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WXR4"/>
    <dbReference type="KEGG" id="dcr:108218704"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000006">
        <property type="entry name" value="endoplasmin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000006">
        <property type="entry name" value="endoplasmin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000005">
        <property type="entry name" value="Endoplasmin-like isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041982651" description="Endoplasmin homolog" evidence="14">
        <location>
            <begin position="24"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="15">
        <location>
            <begin position="100"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="293"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="775"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="293"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="802"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG97767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="815" mass="93304" checksum="446F7B0E8E039566" modified="2024-05-29" version="1">MKTWTVPSVVFLLCLLFLLPDQGRKLHASAEGDSEELVDPPKVEDRLGAVPHGLSTDSDVAKREAESMSRKTLRSNAERFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLSLTDKEVLGEGDTSKLDIQIKLDKEKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQTGGDLNLIGQFGVGFYSVYLVADYVEVISKHNDDKQYVWESKADGAFAISEDTWNEPLGRGTEIRLHLREEAGEYLEESKLKELVKKYSEFINFPIFLWASKEVDVEVPADEDDSSDDDEKSETSSDDDGDDDDSEKGEESDDEKKPKTKTVKETTYEWELLNDVKAIWLRSPKEVTDEEYVKFYKSLAKDFSDEKPLTWSHFNAEGDVEFKAVLFVPPKAPHDLYESYYNANKSNLKLYVRRVFISDEFDELLPKYLNFLKGLVDSDTLPLNVSREMLQQHSSLKTIKKKLIRKALDMIRKIADEDPDESNDKEKNDVEESNENNEKKGQYTKFWNEFGKSIKLGIIEDAANRNRLAKLLRFESTKSNGKLTSLDQYIKRMKSGQKDIFYLTGLSKEQLEKSPFLERLIKKNYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKLGKDSKVKELKEAFKELTKWWKGILASENVDDVKISNRLADSPCVVVTSKYGWSSNMERIMQSQTLADANKQAYMRGKRVLEINPRHPIIKELQARVAKDPKDTSVKQTAELVYQTALMESGFVLSDPKDFASRIYGSVKNSLKISPDAMPEEEEEVEETKAESSVQDDSAKEDDKDVKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WY98</accession>
    <name>A0AAF0WY98_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0415461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG96130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WY98"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="38"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="117"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="336"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="148"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG96130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="415" mass="47114" checksum="4F139ED51FC4AD14" modified="2024-05-29" version="1">MLDKVSLCPDYNTTWQTKESVLQVSRGRKPQDKRRQLVDLKRLLTQCASSASNGDILAAEQTLGTIRSQSSPHGDGTQRLAHYIATAIEARIHGTGTTLYADSCMMGISFADTLKAYQSYFTAVPFQRMSNIFANKAIYRQTMGATKLHIIDFGISYGFQWPCIIQAFSVRPGGSPYLRITGIDFPEPGFLPSKRVAKTGTCLSDYCKRFNIPFEYNCIAKKWDSIQLEDLKIVEGERLVVNCLYKLRQVPDETALESGPRNVVLKLIKRINPELLVLGVLNGSYNSAFFKARFRDALAHFSNLSDMFEATLAHEDKSRLIIEQIMLAKDAINVIACEGTARVERPEKYERWTSRIMKAGFMQTPLPREILAEVQGKVRLQYNKKFLVREDNNWMLQGWNGRVIYALSLWKPVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0WZ66</accession>
    <name>A0AAF0WZ66_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0416937</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG97596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0WZ66"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF3">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG97596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="511" mass="58812" checksum="FC52F4C9CA31FE21" modified="2024-05-29" version="1">MCKIEKRGYIEMGLKSFGGEGKKGIDKFKSSMVSRSKMKLWMIRATTCILLWTCLVQLTTLSEMWGARVLKGWPSCFSHESATAMDVMITPSLPTRVLPPKRVYKNNGYLMVSCNGGLNQMRAAICDMVAIARYLNVTLIVPELDKTSFWADPSEFQDIFDVDHFISSLRDEVRILKQLPPRVKRRVELGLFYTMPPVSWSDISYYHNQILPLIQKYKIVHLNRTDARLANNGLPLEMQKLRCRVNFGALRFTSQIEELGKRVIKLLRQNGPFLVLHLRYEMDMLAFSGCNQGCSNEEVDELTRMRYAYPWWKEKIINSDLKRKDGLCPLTPEETALTLRALDIDRDIQIYIAAGEIYGGEKRMASLTAAYPRVVRKETLLDSSDLGYFQNHSSQMAALDYLVSLESDIFVPTYDGNMAKVVEGHRRFLGFRKTILLDRKLLVDLIDQYNHGSLNWDEFSSAVKEAHSDRMGNPTKRLVIPDRPKEEDYFYANPDECLQSSEMSEERLEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3E2</accession>
    <name>A0AAF0X3E2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA box binding protein associated factor (TAF) histone-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0519022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3E2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
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        <property type="entry name" value="TAF6"/>
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        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
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        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="7">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG99668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="544" mass="60329" checksum="7C7A39B37600E876" modified="2024-05-29" version="1">MSSSNVNNTTSSSSHSLVVGKESIQVIAQKIGNNLSPDIFPSLAADEAIKCMHHSNRTLLTSHDVDFALHLRNLEPIYGFGSGDALKFKKATTHKDLFYIDDKQVDFKNVINAPISKTPRDTSVTAQWLAIEGVQPATPEATLFEALASPSDNKKAEYKDDEISADVKYPVKHALSRELQLYFEKITELTVGRSDSILFKEALLSLATDSGLHPLVPYFTYFISDEVMRNSKSFHLLFALMRLVRSLLRNPHLHIEPYLHQLMPSIMTCLIGKTLSHKLSDNHWELRNYAATLVSSICKRYGHAYHILQPRVTRTLLHTFLDSTKTLAQHYGAIRALADIGPSVVGFVVLPNLEPYLQLIEPEMQVEKQKNETKRYEASRVYGVLLHAVGQCVYKKLKLIPSPLSSPTIVPWKVNSKIMTTMASKRKASMDNIMQQPPLKRVATEGSMGVSSFPVNIQRTAGGYSTGLGGSDVGLSSSMSRQIPTENALGINGRKDDRAKKPSSASTQAWKEVMDAGQSLPLLTECFGESMLTFIPTPLSNIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0X3J4</accession>
    <name>A0AAF0X3J4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Plant intracellular Ras-group-related LRR protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0518971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0X3J4"/>
    <dbReference type="KEGG" id="dcr:108220253"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOG99618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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        <property type="entry name" value="PROTEIN, PUTATIVE (DUF 3339)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11820">
        <property type="entry name" value="DUF3339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7388" checksum="7F042CB07120EF92" modified="2024-05-29" version="1">MADWGPVLVAVVLFVLLTPGLIFQLPGRNRVVEFGNMHTSGASIIVHAVIYFGLITIFLIAIGVHITTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XEB4</accession>
    <name>A0AAF0XEB4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0624168</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH04756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XEB4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042289062" description="RING-type E3 ubiquitin transferase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="493"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="WOH04756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="543" mass="61774" checksum="D314F3BB37CCF66A" modified="2024-05-29" version="1">MASLITNLCLFLSLCVVSIVFAAPRKGLSSMISPAPSSTDEKWQPPSILAWNISGIYKGSWGLMDSKKSHSRSQIFNKSSGDFVLELISTPTTVNGLHSVKGMIIFLDLSDNEHEVGSVEVKVEGVYIWPFRKLRVVAYRGEAGEFGHEKDFICNPYHKLGVFTSQMFEESLWDKIWKRKNSPPHAMEKHCRMELAAHVSLVPPSWQSNGVRYHLDGSMESISVDDDGDCLQTMQLNSTSMNTDMYFLKEMNYNLLVIFISFLQVLLLLQQLSHSSSEFAVGRVSILTIGQQTIMDAYLCALHLVAGIAADSLFCAFATAAFMKFVIFFYFERKYIEAIWKVNGLPAMGRRHDLAVPYSLLCGTFMGGILVMYMFQKFLPFILLLVHSFWMPQIVINVVRDRRGDLHPWYIIGMSVTRLVIPLYFFGCPHNFMQTKVDRHWCICLVVFVGLQALILLLQDYLGSGCAIFCKIFGIKYKHRKRSDQDSDDATMCIICLTAIDFFQSPCVFVVTPCGHFYHAECLQRWMHERTDCPICRSELPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XF15</accession>
    <name>A0AAF0XF15_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0625023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XF15"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877:SF3">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN _ LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH05604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="240" mass="25265" checksum="6B75DE2D81975FE2" modified="2024-05-29" version="1">MASQDQARRKKKSEVGEKQSVSKQFESLTESPERAQNVGKFEMQGEEGEGDIGKYRQAAQQNSIDAIRAAQERYEKAKETATTVKDKAVVSGWGAGEYTAEKVAEATKAAANVTAGAAGYVGEKAVAAKDKVGSVGVSAKDYAAQKLAAATDAVVAAQESAKEYAARKRAEAEREMLAKQSAEAEERHDDIAGAVGEFSQAEPEYGHEGQQGGFGGVLQAIGETVIEIGQTAKDFLVGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XH70</accession>
    <name>A0AAF0XH70_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">DCAR_0727563</name>
        <name evidence="16" type="ORF">DCAR_0830493</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH08126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XH70"/>
    <dbReference type="KEGG" id="dcr:108196196"/>
    <dbReference type="KEGG" id="dcr:108199524"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="WOH08126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOH11016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="447" mass="50366" checksum="7EF59E17B2A3A7E5" modified="2024-05-29" version="1">MREILHIQGGQCGNQIGAKFWEVICAEHGIDSTGRYQGDTDIQLERINVYYNEASSQRYVPRAVLMDLEPGTMDSLRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALSVPELTQQMWDSKNMMCAADPRHGRYLTASAVFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEEGDYFEDEEEEGQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHH9</accession>
    <name>A0AAF0XHH9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0626523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHH9"/>
    <dbReference type="KEGG" id="dcr:108226359"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="172"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="617" mass="68209" checksum="71C037BBED8E4B78" modified="2024-05-29" version="1">MGFCKRVVYKPVAEVDVSSSSDEVYISANVKAPRMAGLLVKLFVWLLELRILKPLLLYMLKRENQVHKFVSFVELEDPPLYVPLHPYEEHREPETKYLESGLSPSEQVQQATECIEFSENIGENEKISFRHWTILDYSRAYLSRQITPNMVADKFIASVHQSNDPALQMSFFISCDDQEILRQANEATLRYERGEPLSALDGVPFAVKDEIDCMPYPTTGGTKWLHKVRPCTDDADCVKRLKLCGAIIVGKTNMHELGAGTSGINPHYGAPRNPYDTNKITGGSSSGSAAVVSAGLCPAALGVDGGGSVRIPASLCGVVGLKPTFSRVSHSGVLPLNWTVGMVGVLAGTVEDAMIVYSAISGQILSHQHVEQRHPEVHLPLLNSPNCMPKIKLARYDKWFNDCSSDIKLSCSNALDQLQKIYGWETVDINVPEIESMRLAHYITIGSECNASLSRYLEKLDMDEVGWDARAALSLYGAFDSKEYLNAQRIRNRQLQIHQKIFDQADVIVTPTTGVTAYTIQDDAQKTGELDYINGASLVRYMIAGNFLGLPAVTVPVGYDRDGLPIGLQIIGKPWSEASLIYIAYAVQGLCAGEYKKPEVYYDLLSGLKKGETTQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XHI8</accession>
    <name>A0AAF0XHI8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0727365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XHI8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF2">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH07930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17932" checksum="74A64ABC28680AAB" modified="2024-05-29" version="1">MIIKDELPFRFVERDGFKKFCEVSMPEFKIPSRFTIVKDCYSLFVAEKKKLFDIFASQCQRVSLTTDTWTSIQNVSYLCLTAHFIDSNWRMHKRFLNFCVIKSHKGLNIGNVVDFCMEEWGLKNVMCITVDNATSNDKALTQLKKKLIKRGALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XIX6</accession>
    <name>A0AAF0XIX6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_0727296</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH07862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XIX6"/>
    <dbReference type="KEGG" id="dcr:108193335"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000225">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF47">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="100"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOH07862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="268" mass="28711" checksum="3DF1CBD7ABE5BE7F" modified="2024-05-29" version="1">MVVGAANISRGGGSFLVSYLRRAGGLRPHFPASFPVPIPKAPFNFPSLKATTTTSNKISRVCFCTTSGSVPSTTDTTTVVSDSTSDTEAVISEEDKLKGLGNSLDIRVGQIIKIWRHEEADSLYVEEVDVGEPEPRIICSGLVKYIPLEHLQDKKVVVLANLKPRNMRGVKSSGMLMAASDAAHENVELLVPPEGSIPGDRVWFGSEEEMGSLPDAASPNQIQKKKIWESVQPHLKSDASCVAALGMHLMRTSAGVVETGSLKNANIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XJX4</accession>
    <name>A0AAF0XJX4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0728830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XJX4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH09373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="449" mass="50471" checksum="72CFCF523C043C4F" modified="2024-05-29" version="1">MPCLNVAVIGAGIGGLCAGRKLQRVGHRVTIFEKQNQLGGTWVYDPRVESDPLSLDPDREIIHRSLYASLRTNFPRHVMGFSDFPFTKIYEDSRAFPSHEEHLRFLNDFSEQFGLVELTQLNTEVVRVELKEDEWVVESRMGELTREEGFDAVVVCVGHHTEPRVANFPDIGQWPGKQIHSHNYRVPEPFHNQIVVMTGAGPSAYDISRDIAGVANYTTTKLASDNTSQTTDIDYVDESGRVAFVDGSSVCADIIFHCTGYMFSFPFLRTNGIVTVDDNRVGPLYKHVFSPEIGSRLSFVGIPCNITLFPMMELQSKWISCVLSSKVVLPSKEAMLADTNEHYRSLEERGIPKHSTHSLATTAFQYLDWLASQVKVQPVEERLKNIYKQLVSVFFSSEGDQIREWDVDSWVNGNHLKLLAASEKHSASKLLAAFEENVGHDNSKVVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XKP7</accession>
    <name>A0AAF0XKP7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0729371</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XKP7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="2">
        <location>
            <begin position="188"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="3">
        <location>
            <begin position="401"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH09911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53603" checksum="12AEE39788B51881" modified="2024-05-29" version="1">MPSSHGHEVTVDRAILLFGIVSGKYIDLGHVIHQGILRFLQGGTTGAIPYGTIVTKLCRSSGVRWPANEQLQLPATPIDHSAISRMTEWDGGVPHPRGLGYLYDEMPGGRPAFVRREQTRASGAGTSQTERSSEPMGDVHYRRLARRMDTMHDIHQRRVRTFFGTLAFNYALISKPLTDLLKVGAFKWTAEAQDSFEKLKRALTSALVLAVPIFEETFVIETDASKKGIGAVLMQRNHPLAFISRSLGPKWQKLSVYEKELLAIVFPVQKWEQYLSDGHFIIRTDQKSLKWLLQQKVSTPFQQFWLSKLLGFDYEIQYKHGKENLVADALSRAKGAEILYLAIFVIASNVEDLIKDSYKGDQWIENILQSFQRSETVKHFAVIDGLLRKHNKIVVGISGDIQTKLINWHHSSAEGGHSGRDLTMKRLKHLFYWKDMTVQVTQFIQKCQVCQRNKADLAAYPGLLQPLRIPNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XMD4</accession>
    <name>A0AAF0XMD4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">DCAR_0728871</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP093349">
        <property type="protein sequence ID" value="WOH09414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XMD4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10289">
        <property type="entry name" value="GST_C_AaRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000070">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000004">
        <property type="entry name" value="Glutamyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione S-transferase C-terminal" evidence="14">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="15">
        <location>
            <begin position="218"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="16">
        <location>
            <begin position="525"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="17">
        <location>
            <begin position="628"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
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</entry>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Sanseverino W."/>
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                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CIA30"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="162"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="2">
        <location>
            <begin position="304"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="3">
        <location>
            <begin position="460"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH11604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="64898" checksum="7E19D82701232FB8" modified="2024-05-29" version="1">MTCAACSLTPTTSPAASFNKSIIQKWSLNNGAVLFYKKPASSILTRPCAASSSSSSSATTKKKRKKKKKKDEEEEEEEEEKDESRSGDDESSSSMMKRLDDVNPVGLGRKSRQIFDEVWRKFSGLGQISRTIRSDEDDEAVGGPMCEFAIPGAQNTTVLVVGATSRVGRVVVRKLMLRGYNVKALVRKASQQVVDMLPTSVEIVIGDVGDSLTLKAAVENCSKIIYCATARSSITGDLNRVDNQGVYNLTKAFQDYNNKLAQLRAGKSSKSKLLLAKFKSEDSLSGWTVSKGTYFQDVVATKYDGGMDAKFEFTENEEATFSGYVFTRGGYVQLSKRLSLPLGSTLDRYEGLVLSVGGNGRSYVIILESGPSADTTQSKMYFTRISTKAGFCRVRVPFSAFRPVMPEDPPLDPFLVHTFTIRFEPRRQRVTDGTTGGNQDLRSFQLILEYIKALPTGQETDFILVSCTGSGIEANRREQVIKAKKAGEDSLRRSGLGYTIIRPGPLMEEPGGQRALVFDQGNRISQGISCADVADICVKALHDSTARNKSFDVCYEYVAEQGKELYELVAHLPDKANNYLTPALSVLEKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XRQ6</accession>
    <name>A0AAF0XRQ6_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">DCAR_0831576</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XRQ6"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000004">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041908300" description="Receptor-like serine/threonine-protein kinase" evidence="13">
        <location>
            <begin position="23"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="15">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="16">
        <location>
            <begin position="342"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="502"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="WOH12077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="820" mass="92359" checksum="CE031419A17A3693" modified="2024-05-29" version="1">MQLPSIILVCSILSSTLIKSMAVDTISMHQTFRDGDTITSAGGEFQLGFFSPGSSTNRYLGIWYKKISRVTVVWVANRDSPLVNTSGFLQISREGIILQAVNSSTGIIWSWSTSKLFKNPVLQLLDTGNLILRDEDRDFKSEEDFIWQSFDHPGDNMLPGMKFGIDLVTGMNRISSSWTSVNDPSRGSFTIGLDTSGFPQILLSKGSVIQARTGRWDGYKFKGIPKRSLNGIFKDEFVFNEKEIYYRFYLVNPTSATMRIILTPTGNVKVLVWNDQQQIWTVYSSLMDNDCDRYGLCGGYGICKIDKTPRCECLRGFVPRSQEKWKAADWSSGCIRKTNLVCGTDEGFVKYSGVKLPDMRSSWYTLKMSLQECEKLCLKNCSCTAYANADARRGGHGCLLWFGELIDISDYAEDGEDIYVRMPSSELVKSRKGKLEFILITAVLLVVLVGLILLSVYKKRKLMKEESLKLDSESVSLTKIENEDLELPLYDFESIAHATSNFSPEKKLGEGGFGPVYKGTLTGGLEIAVKRLSKDSSQGLDEFKNEVSCIAKLQHRNLVTLLGCCTEKGERILIYEYMANKSLDSFIFDSNARNTLDWPKLYNIITGIARGLLYLHQDSKLRIIHRDLKASNILLDHDMNPKISDFGMARSFGGNETESNTSRIVGTYGYMSPEYAIDGQFSVKSDVYSFGVLLIEIITGVKNRLFSHPDHSLNLLGHAWLSYEQDNLLQLIDGVILESNNYSEVFRVIQIGLLCVQHDPEDRPVMSQVVLMLSSNMKLPHPKQPGFFMERYLLRQDQFLSKPNLSSSTQLTITTLLPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XSU1</accession>
    <name>A0AAF0XSU1_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">indole-3-pyruvate monooxygenase</fullName>
            <ecNumber evidence="10">1.14.13.168</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0832179</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
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            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Plant hormone metabolism; auxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.168" evidence="10"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103075">
        <property type="term" value="F:indole-3-pyruvate monooxygenase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF49">
        <property type="entry name" value="INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0073">Auxin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOH12672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="411" mass="45749" checksum="096A53C588C316A0" modified="2024-05-29" version="1">MVHNLEHEDHFFAGRCILVNGPVIVGAGPSGLAVAAGLKQQGVPYVILDRADCIASLWQNRTYDRLKLHLPKQFCQLPYYPFPETFPEYPTKHQFIEYLESYAKRFEIGPRFNESVMSAKYDKTCGLWRIKTTMLKCPGRGEIEYICRWLVVATGENAEKVVPEFEGLEEFGGNVMHACDYRSGGVYKGKRVLVVGCGNSGMEVSLDLCHHDAIPSMVVRSSVHVLPREIFGRSTFELAVSMLKWLPIWAVDIILLASARLLLGNIEKHGLKRPATGPLQLKDSTGKTPVLDIGALQKIKCGEIKIVELVNGTKLEIDSVILATGYCSNVPSWLNENEFFTREGFPVAPFPNGWKGKLGLYAAGFTRKGLSGASLDAMRVAQDIGKLWKEETKQKKHSVAVAGLKRYKSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XTD7</accession>
    <name>A0AAF0XTD7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0933466</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XTD7"/>
    <dbReference type="KEGG" id="dcr:108200723"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
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        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF22">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14Z_US14Y_US14X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOH13953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6357" checksum="A789AF2B3D0EE95D" modified="2024-05-29" version="1">MGHSNIWNAHPKTYGPGSRACRVCGNPHGIIRKYGIMCCRQCFKTNAKEIGFVKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0XU00</accession>
    <name>A0AAF0XU00_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">DCAR_0933473</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZFPL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH13960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0XU00"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058730">
        <property type="entry name" value="U-box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039043">
        <property type="entry name" value="ZFPL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058731">
        <property type="entry name" value="Znf-B_box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
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            <begin position="321"/>
            <end position="341"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
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                <person name="Simon P."/>
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    <sequence length="325" mass="35476" checksum="7A10658381EDA444" modified="2024-05-29" version="1">MADCSLFLQQRSQLPKFGNWESEDNVPYTVYFENARKGKSGKKTNPSNPDMVSNNSSPVQVTSAGKAELEVVNGPETGRPGHKRHMSREDGEPIPGPITDIQSSHQQHGQVTSENNYKSEIEANKVPDGTGSSHQHWLSGEDGDIKKPSNSPLRHDTIGKKDHADSPHHRLGSGGTSSPKRVSRQNLRPDRSVEQSPLHPHHQTRTGNKGSGVSSPSWERKGSSEGTHGLAPFTPGRSRLRSSVTGGDETPDHGPVVPKFGDWDESDPSAGEGYTHIFNKVREEKQSETGKVPVVPAETSYTSNGQKQYANESSKGCFCFPWGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0Y1L5</accession>
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        </lineage>
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                <person name="Senalik D."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
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        </reaction>
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    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF571">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GST_NTER"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH16494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="26804" checksum="CB44DD744BAC1997" modified="2024-05-29" version="1">MSTSDVKLLGRCASPYVNRVQIALNVKSIKYEYLEENLSSKSSLLLNANPVKKKVPVLIHGNRSVSESLVIVQYIDEVWTSGPCILPSDSLDRAEARFWAAYIDDKWFPLATEFKLTRQEEKKLVVKQKIIEGLMLLEEVFVKCSKGKAYFGGDSIGYLDIVLGSFLSWLTAIELTSDVCFLDMTKTPVLVGWSDRFRSNDAVKNIVPGTEKVIELLNVYLKLSSTLKAYAYVFNQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AI26</accession>
    <name>A0AAF1AI26_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">DCAR_0100342</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG81197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108192556"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000508">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF189">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="949"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1000"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1098"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1192"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="323"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="633"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="964"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1281"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="WOG81197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="1527" mass="170430" checksum="85E84BB3DE297FA5" modified="2024-05-29" version="1">MGISNFRYLVAENYLLGSVYSLPTLELISICFNLILCVVFLLSRQISVYINRIRINKEDTDEISGPTRRNVDAEIQSVEIGKWYKASAFCCFYILVVQVLLIGFDCVKLIRGSSGGRGSDWILLLFPAVQGLAWFGQSFWVLYCKIKPTEKLPLLLRVWWAVSFAFCSCTIYADGKALVTEGAKHWNSHVLSNFATTPAISFLSIVAARGVSGIKISRASDLQEPLLEEEEDAGCLKVTPYKDAGIFSLITISWLNPILSLGAKRPLELRDIPLLAPKDRSKTNYKILNSNWEKLKATNPSNQPSLAWAILKSFWKEAACNGIFAGVATIVSYVGPYMISDFVDYLGGIETYPHEGYILAGTFFIAKLLETLITRQWYIGVDILGMHVKSALTAMVYRKGLRISSLAKQSHSSGEIVNYMAVDVQRVGDYCWYLHDIWMLPLQIILALCILYKNVGLASIATLISTIISIVATIPLARIQEDYQDKLMASKDERMRKTSECLRNMRILKLQAWEDRYRLMLEEMRNVEFKWLRKALYSQAFITFIFWSSPIFVSAVTFATSILLGGELTAGRVLSALATFRILQEPLRNFPDLVSMMAQTKVSLDRLSGFLLEEELQEDATVSLPRGFTDVAVEIIDGEFCWDPSSSSSSRPTLSDIHLKVEKGMRVAVCGMVGSGKSSFLSCILGEIPKTSGEVRICGSSAYVSQSAWIQSGNIEENILFGSPMDKAKYKSVINACSLKKDLELFSHGDQTIIGDRGINLSGGQKQRMQLARALYQDADIYLLDDPFSAVDAHTGSELFKEYIMSALATKTVIFVTHQVEFLQATDLILVIKEGRITQAGKYDELLQAGTDFDALVSAHHEAIEALDIPVQSSADSSEFHSLDEEIQLSDKCESIIGNAQISAKEVQEIGTSSDQKLIKEKKKAKRSRKKQLVQEEERERGRISMRVYLSYMTAAYKGLLIPLIILAQTLFQVLQIASNWWMAWANPQTAGGQPKTSNIVLIVVYMALAFGSSCFIFARAVLVATFGLAAGQKLFLKMLTNVFRAPMSFFDSTPAGRILNRVSVDQSVVDLDIPFRLGGFASTTIQLLGIVGVMTQVTWQVVLLVIPMAIACLWMQKYYMASSRELVRIVSIQKSPIIHLFSESISGAATIRGFGQEKRFMKRNLYLLDCFARPFFCSLAAIEWLCLRMELLSTFVFAFCMILLVSFPHGSIDPSMAGLAVTYGLNLNARLSRWILSFCKLENKIISIERIHQYCHIPSEAPQVIEDSQPPPSWPEEGTIELNDLKVRYKENLPLVLHGVSCTFPGRMKIGIVGRTGSGKSTLIQALFRMIEPEGGRIVIDDIDISRIGLHDLRSRLCIIPQDPTLFEGTIRGNLDPLEKHTDSEIWQALDKSQLGEIVRLKEQKLESPVLENGDNWSVGQRQLVSLGRALLKQARILVLDEATASVDSATDNLIQKIIRTEFRDSTVCTIAHRIPTVIDSDLVLVLSDGRVAEFDTPAQLLDDKSSMFFKLVTEYSTRSSGIPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AJS9</accession>
    <name>A0AAF1AJS9_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0102231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
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                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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            <dbReference type="PubMed" id="27158781"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <sequence length="337" mass="37764" checksum="AA896B8FCD2BFB61" modified="2024-05-29" version="1">MGTGIGCEHKNLMNIELKQGQELANQLKEQLCSPKRSKETCEILVATILSSFEKSLSILKSSALKLQDDSMLNSLHSSPGDASPRSEMPDEPRKNVFKKRKAMTQWSEQVQVPFGPGIEGPVGDEYSWRKYGQKNILGATFPRAYYRCTYRNSQGCLATKQVQRSDESLSLQVIYKGRHTCKKGSESSAGSASCGNQEKRHKIQKQNVENTINFGTCDKTEELGRCIQVERMPSFSFPSTNHFCPEDTREYSFIDSRYLPSLVNQETSECSYPSLSPYHNMNNFEIGQNLGSSVSSLAEILSTPNSVTNSPVCNFDLLIDPVDFDPNFPLDILEFFS</sequence>
</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
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    </dbReference>
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        <location>
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            <end position="20"/>
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        <location>
            <begin position="21"/>
            <end position="617"/>
        </location>
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            <begin position="544"/>
            <end position="565"/>
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        <location>
            <begin position="21"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG88920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="617" mass="67422" checksum="6867347D2834C69C" modified="2024-05-29" version="1">MVLSLSLSLPLSSLLHKAFHIDCGGSTQSTDKFNTTWLADEFFSAGATGLVSEPLRFQNLHEKTVRFFPISSGKKNCYTVPNLPIGSRFYIRTFTVYDNYDGKSHSPSFEVSVEGTLVFSWHSPWPEDISRSGAYSDLIAFISDGEADICFYSIATDPPVIASLELVQIDAAAYDAASIGNSSILVNYGRLTSGSGQWGPGFSNDTDLFFRSWQSDADFRSENSVGIKSVSAVKKVINTDRSPNFFPEKLYSTAVVAKGKGGLEYELQVDAKMDYLLWFHFAEIDVSVNRAGKRVFDIVVNEINVTRIDIYKEVGGFAAYDWHYVVKNLSDTILSVKFVPVVGSPLICGVENYAIVPADLKTIPDQVIAMRSLKESLKVPDRMGWNGDPCAPTSWDAWEGVTCHTNKGESALVVSQIDLGSQGLKGYISEEISLLSNLVSLNLSSNTLGGTLPLGLGQKSLVKLDLSSNKFSGSIPESLTSSKLQLVLLNDNSLEGQVPEEIYSIGVHGGAIDLSGNKGLCGVPSLPDCPLFWKNGGLSKNGKIAIGISCLIFVCLLSLLIYICCIRRGRNDYDFGLPHELTSLAAKRNRYHRQKSLMTLEMESQHAKGFIPSYSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ASG7</accession>
    <name>A0AAF1ASG7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0309889</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG90645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ASG7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042885">
        <property type="entry name" value="HIPP47/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46932:SF12">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG90645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14799" checksum="4E45B5019E5174FE" modified="2024-05-29" version="1">MVKSLLKVVIRVVMEDQKKSRKKAMKIAATAFGVESVALTGDDRDQIVVVGEGIDTVELTNSLRKKVGCADLLSVGPAKEEKKEEKKEEKTSESLAVPLAYGSQPYYYNYNQYPVVYGYDRVDQRDSAPCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATK8</accession>
    <name>A0AAF1ATK8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="12">HMG-CoA reductase</shortName>
            <ecNumber evidence="12">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">DCAR_0311130</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="12"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG91875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATK8"/>
    <dbReference type="KEGG" id="dcr:108211606"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016135">
        <property type="term" value="P:saponin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000002">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF43">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="WOG91875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="571" mass="61759" checksum="2224BF68778DD72C" modified="2024-05-29" version="1">MDSHRSLPPKPPRATLHHRRKTVDLSPTPPPPVKANSDALPLPLYLTNAAFFTLFFSVAYFLLHRWREKIRTSTPLHIVTVTEIVAVVSLFSSFVYLLGFFGIDFVQSILYRPYIEDHEDEEIFTLMDHNNNNSNNDISNDVSNDKSKPVGVEENEEIINSVVLGKTPSYSLESKLGDCYRAALIRREAVQRTTKRSLEGLPLDGFDYNSILGQCCEMPIGYVQIPVGIAGPLLVNGCEYTVPMATTEGCLVASTNRGCKSIYACGGATGILLRDGMTRAPVVRFPTAKRAADLKFYLEEPLNFDTLAVVFNKSSRFARLQRIQCSMAGKNLYIRFTCSTGDAMGMNMVSKGVQNVLDFLQRDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAIITGDVVKKVLKTTVPALVELNMLKNLAGSAVAGSLGGFNAHAANIVSAIFIATGQDPAQNIESSHCITMMEAVNDGQDLHISVTMPSIEVGTVGGGTQLASQSACLNLLGVKGASKDSPGSNSRLLSIIVAGSVLAGELSLMSAISAGQLVKSHMKYNRSNKDISTIACQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1ATR3</accession>
    <name>A0AAF1ATR3_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0311360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG92101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1ATR3"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000023">
        <property type="entry name" value="protein REVEILLE 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF155">
        <property type="entry name" value="DEUBIQUITINASE MYSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="79"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
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        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG92101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="526" mass="58390" checksum="4905682025506FE4" modified="2024-05-29" version="1">MCVALTLPLKKHWLVDYHQFVESMGSATDDIQIQSENARSRMTSPDTLTAGLQSPADTQDKCQVVSSDDYAIKVRKQYTITKQRERWTDEEHHKFLEALKLYGRAWRRIEEHIGTKTAVQIRSHAQKFFSKVVRESSINEKNTVKPIEIPPPRPKKKPMHPYPRKMVGRVEPGAHLSDELGRSASPSHSVSGRENRSPASVLSAVGSEATCVTDSNMQNCIFSASSSDADDQTNSVFPSDTTPLPEENRSENGSNSPIQANDSSNEDEHVPTKLELFEADGSFVKEESNDEVSTHSVKLFGKTVTVAESSLSSSRNNSCGSSHLESADERPVQTSPWNISQGMSYASPDAAEYTWTGVPCKAPAVYYMQYAINNSINVDDESSVPVPCWTFYGGVPYPFLQLRNSVQERVYMHFDGSNVQDKVLQKPGSLTGSNSGSINVETDGDKTWEMETQCSQSLSEKKDAEQKLTSPLKVRADAGFTEQKSILAKANHGKGFVPYKRCLAERDNGTSISGEEREERRTRLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AUH8</accession>
    <name>A0AAF1AUH8_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable 6-phosphogluconolactonase</fullName>
            <ecNumber evidence="5">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0414763</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP093346">
        <property type="protein sequence ID" value="WOG95444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AUH8"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF9">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOG95444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="295" mass="33434" checksum="FBDB135E9178859E" modified="2024-05-29" version="1">MRTNNVIQKKLLSEEEVSVELAQYVIYLSDKCIKAKDSFTVVLSGGTLIHTMRKLVEKPNADCVDWSKWFVFWLDERVVSLEDPDSNYKLAYDGFLSKVPIPDGNIFAIDDELSTEEAAEEYEARLRVLVDNRTIGLSSCTGFPKFDLVLLGMGPDGHVASLFPWLYQRYEEERWVTFVKDSPKPPSSRITMTFPVINSSSECALVVTGKQVAYAVKVALASRESNFIPLPVEMVYPEGNLTWFLDKDALSQLSNINDHSYNSLFLGLGFLVLIITCLLYYFPCIHIFLALLKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AVC4</accession>
    <name>A0AAF1AVC4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">DCAR_0313189</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG93901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AVC4"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000177">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000015">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042019943" description="Protein kinase domain-containing protein" evidence="19">
        <location>
            <begin position="23"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="135"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="319"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="WOG93901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="68982" checksum="98BF2E0F4E4C96D9" modified="2024-05-29" version="1">MHFSLSSILLISLSCFVNRSFSDHADTSLACSKKTVLDPSERQGFMTSYLLAMVSLTESVSMQSYGAAENGTVFAFADCRRDLSKNDCILCVEHCKVKLHSCLFFQSVFLGGRVYNNFDGCFLRYDGYRFFGEISDDEVFCAGNEFAGNKSLLRESVGELVRNLGVKGAKNEGFSVGNYNKGDFQVYGLVQCWRFLNKTDCEKCLEGLVAKVGSCLPKTEGSVLNSGCYVRYSTHKFYHNSRTTAASIQTKGNKHLTLVLAGISAGIASLMLASTTYFFLKKHMEKQRREKEQYDYLASVTDSKLIFSYEMLEKATKYFHDSNKLGEGGSGHIYKGRLPDGRTVAIKRILFDTKQWAEHLFNEVNLISGINHKNHVKLLGYSVTGPESLLVYEYMPNQSLHDYFFGKKNVEALKWDVRYNILLGTAEGLMYLHEDSNLRIIHRDIKLSNILLDKEFTPKIADFGLARLFPHDKTHLSSAIAGTLGYMAPEYVFSGRLTEKADVYSFGVVVIEVVCGRQNYAFSESTIPLLEMVWNSYQKGKLCEEIDPVVKASFQEDAERVLKIGLLCAQASAELRPSMSKVVKMLMNNQQLPQPTQPPFISSRYVEKIPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AW08</accession>
    <name>A0AAF1AW08_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PFK</name>
        <name evidence="17" type="ORF">DCAR_0313598</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by AMP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AW08"/>
    <dbReference type="KEGG" id="dcr:108213009"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ATP_PFK"/>
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        <property type="entry name" value="PKF_sf"/>
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        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
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        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
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        <location>
            <begin position="131"/>
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        </location>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="490"/>
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        </location>
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            <begin position="1"/>
            <end position="11"/>
        </location>
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        <location>
            <begin position="20"/>
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        </location>
    </feature>
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        <location>
            <begin position="498"/>
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        </location>
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        <location>
            <position position="137"/>
        </location>
        <ligand>
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        </location>
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            <position position="227"/>
        </location>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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    </evidence>
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</entry>
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
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        <property type="entry name" value="AMPK1_CBM"/>
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    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
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    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF2">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="77"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="290"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="WOG96437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="65449" checksum="15A520272EA7F9DB" modified="2024-05-29" version="1">MSSLTLPNSYTTFQQSPQHLSKKTVYDCAIWGRDLCLRNGGSVSPGCVRNKGYFRVHAMSTSSSSSSALKMNLNEYMVTLEKPLGIRFALSVDGSVFVHSLKKGGNAEKSRIIMVGDTVKKASESSGGKLIEIKDFGDAGNLTKEKSGSCSLVLERPFSPFPIHHLHPLNDLDLLFNKGHVPVTTWNKKIMASNLRASADTSGNSGFVVFNSKFLNSKGWNCLNAETGQAQSKLQNSTHVEPISQLVTMFSDEESGDIEWAHGNFPLEEYIEALDRSKGDLYYNHSLGMRYSKITEQIYVGSCIQNENDVETLSGVGVTAVLNFQSVTEAQNWGINSKLINESCQQSNILMINYPIREVNSFDMRKKLPFCVGLLLRLLKKNHRVYVTCTTGFDRSPACVIAYLHWMTDTSLHAAYSFVTGLHTCRPDRPAIAWATWDLIAMVEDGKHDGPPTHAVTFVWIGHEGEDVSLVGDFTGNWKEPIKAIHKGGPRFEAEVRLSQGMYYYKFIIGGQWRHSTASPTERDERGNLNNIIVVGDIASVRPTSRQEKKDANVVKVIERTLTENERFMLAKAARCVAFSVCPIKLAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AY86</accession>
    <name>A0AAF1AY86_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">DCAR_0518482</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AY86"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46368:SF7">
        <property type="entry name" value="GFO_IDH_MOCA-LIKE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="139"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="WOG99134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40187" checksum="2B599EAAC7A8C973" modified="2024-05-29" version="1">MAEHTKAIRFGIMGCAKAATKVSRAIKMSPNSTLYAIASRSLEKAQQFATENGYSDQQVVIYGSYSELLDDPLVDAVYMPLPTSLHLEWAVLAAKKHLLLEKPTALDATQLDRILEACRSNGVQFMDGSMWYHHPRTAKMKELLSDSKLFGQVKSIYSSSSYVGGQGFLENNVRIKADLDSLGALGDAGWYCVGGILWAMNNNLPTTVTALPSVVRNSDGVLLSCTASLHWEKEEIVATFFCSFLAHETMDLTISGSNGTLHLEDFIIPYEETSASFKYTSGAKFVDMHIGWNVKPEEVQVDTGELPQEARMVQELSRLIQGIKYSGRLPDQKWAEVSRVTQLVIDTVIRSINYGFEPVYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1AYU7</accession>
    <name>A0AAF1AYU7_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0519135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1AYU7"/>
    <dbReference type="KEGG" id="dcr:108220424"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041775913" description="Expansin" evidence="7">
        <location>
            <begin position="24"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="47"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="172"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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</entry>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Macko-Podgorni A."/>
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        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BBL7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036574">
        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH13475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7222" checksum="AB5AC0F1CD4FB2CE" modified="2024-05-29" version="1">MNTKIERILVAIITRHLVIGLDQENCIGACNQFPNCAYQCIHKGYPSGDCTTKGHDPPCACCCNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCM7</accession>
    <name>A0AAF1BCM7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0830843</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM192 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH11361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCM7"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029399">
        <property type="entry name" value="TMEM192"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31592">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31592:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14802">
        <property type="entry name" value="TMEM192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH11361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="355" mass="40629" checksum="144E01D7BEED0B0D" modified="2024-05-29" version="1">MSSDRHASSSTISPAEENDLFLDILHEAPLFGHRKPTNIVGSVFYCLVLVGYAALAVGGPWIFHHIQELESPLLSSSSVALLFLTGIFQQYMVYQVQKIRLQGYYMFSQKLKHIVRLPFASTAYGTAAMLLVLVWKPYIGILSMSTLLRIIMLIEVIFAGFFMSLYIGYVHRYNSLDSQPDVLKSLYSPLQPSSSLEGLRYHDGSRLSDQQMALLQYQRENIHFLSEEILRLQECLSKYEKSSDGITPQVDLAHLLAARDQELRTLSAEMNQSQSELTLARSLIAERDSEIQRFYTTNNQYVEENERLRGILAEWSARAAKLERALEVERMSNMELQKKILRTKKKTKDEFVEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BCV2</accession>
    <name>A0AAF1BCV2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0934227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093351">
        <property type="protein sequence ID" value="WOH14705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BCV2"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF467">
        <property type="entry name" value="CARBOXYLESTERASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH14705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="144" mass="16075" checksum="F5CAC9825C87E680" modified="2024-05-29" version="1">MAIRVGSEKPEGYNVEGIVLVHPYFWGREALSGEPVEPQSRDFLDRLWSFVKPSTRGLDDPLIDPSKDPGLSSLGCKRVLIFVAGKDVLKQRGLFYKEVLDKSGWGGQVEVVETKEEDHVFHLFNPTTDNAVSLVRSVASFVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF1BD48</accession>
    <name>A0AAF1BD48_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_0832189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF1BD48"/>
    <dbReference type="KEGG" id="dcr:108199508"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043436">
        <property type="term" value="P:oxoacid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000010">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="6">
        <location>
            <begin position="90"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="6">
        <location>
            <begin position="362"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="WOH12682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="510" mass="55482" checksum="E9946AE4A7A1D7D1" modified="2024-05-29" version="1">MASAAIASSSSSFINQRRNDVGLSSFSPNTSFYLSNAKKKMVSKNIVSVMAPQSQRTPSTTGSVKTGMTMTEKILARASEKPQLSPGENVWVNVDILMTHDVCGPGSIGIFKKEFGQNAKVWDREKLVIIPDHYIFTKDERANRNVDILRDFCNEQNIKYFYDINDLGNFKANPDYKGVCHVALAQEGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRFVMDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLSMEERMTLCNMVVEAGGKNGVIPADATTYKYLEDKTSLPYEALYSDEKARFLSEYRFDISKLEPLVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPASPSCGACLGGPKDTYARMNEAQVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPREFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161YLZ4</accession>
    <name>A0A161YLZ4_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0520933</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH01549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161YLZ4"/>
    <dbReference type="Gramene" id="KZM95061">
        <property type="protein sequence ID" value="KZM95061"/>
        <property type="gene ID" value="DCAR_018303"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108221626"/>
    <dbReference type="OMA" id="DNWHLSW"/>
    <dbReference type="OrthoDB" id="2014623at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF24">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="COBRA C-terminal" evidence="9">
        <location>
            <begin position="416"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOH01549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="654" mass="71867" checksum="14C5BDD3BFFC452D" modified="2016-07-06" version="1">MGKFHNSLCIYKLLILAVLHTTICFSQAQAPQNSPDQCNEILVTYTYVSGYAVPPMVPSDLSQQAYRFQSTLTVLNNGLQELPSWRVFVGFRNNEVLVSASNAVLADGTSLPANVSTGAILAGFPVTNLRTGIQTAGDFNLMLAQVDLVGTQFGVAAPDVPLPGSLRIANDGFSCTSPFAQGNASQVCCFKDSDSPPSFELEDQEIFEPLQNGDISIMYDVIRSYESNYLAQVTISNQNPIRRLDNWHLSWEWMREEFINTMRGAYPFIVDTNECIYGRQGEFYQDLDFSAALNCERRPVIIDLPLERTNDTNLGRIPFCCRNGTILPPNMDPSKSMSIFQMQVYKMPPDLNRTQLTPPQNWKINGTNSPNYQCGPPVRTNPTLFPNAEGLSSESVAIASWQVVCNITRSASESPKCCVSFSSFNNDSVIPCNTCACGCNSDQSDVCSATTPALLLPSQALLVPFDNRTKMAKEFSKINNRKMPDPLPCPDNCGVSINWHLLSDFKDGWSARMTVFNWGETDFADWFASVQLDNAMPGFEQAYSFNGTALSSSDQILFIQGLPGLNYIVSERDGKNPRKDPRVPGSQQSVISFTKKTTPGINVPRGGGYPSKVYFNGAECSLPSMLPSDGYGITAATSVFSCLIASFVLLLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A161ZV90</accession>
    <name>A0A161ZV90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_020713</name>
        <name evidence="8" type="ORF">DCAR_0625640</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000006">
        <property type="protein sequence ID" value="KZM91922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH06217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A161ZV90"/>
    <dbReference type="STRING" id="79200.A0A161ZV90"/>
    <dbReference type="Gramene" id="KZM91922">
        <property type="protein sequence ID" value="KZM91922"/>
        <property type="gene ID" value="DCAR_020713"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108226595"/>
    <dbReference type="OMA" id="KTYANYH"/>
    <dbReference type="OrthoDB" id="1426517at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415:SF51">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31415">
        <property type="entry name" value="OS05G0367900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="72"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM91922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH06217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="208" mass="23370" checksum="3F16D28B734D7E13" modified="2016-07-06" version="1">MASYYENKRRKKILKRVLFGTLIFLFVVGLAVLITWAILQPKKPRFILQDASLYAFNVTANPTLLTTNFQLTISSRNPNSKIGIYYDKLHVFAAYRSQQITYYTTLPSNYQDHKEVKVWSPFVYGTNVPVAPYNGLALGQDQANGAIWMMIKINGRVRWRVGSITTGSYNIHVTCPAYIPFGNNNAGTVVTVGGVIKYQLSVSCEVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A093</accession>
    <name>A0A162A093_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_016315</name>
        <name evidence="9" type="ORF">DCAR_0518665</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM93070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOG99317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A093"/>
    <dbReference type="STRING" id="79200.A0A162A093"/>
    <dbReference type="Gramene" id="KZM93070">
        <property type="protein sequence ID" value="KZM93070"/>
        <property type="gene ID" value="DCAR_016315"/>
    </dbReference>
    <dbReference type="OMA" id="DDHICER"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF56">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KZM93070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="WOG99317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="420" mass="46383" checksum="6DA38F0971C36E27" modified="2016-07-06" version="1">MEIKEDDDKAEGKKLCARNKHGDGVSWYRGALIGKGNFGCVYLANLKKPRSKFSCFPCVMAVKCAEVSISGSIQKEREILDNIGRCGYVIRCFGEEITNGENGEMVYNLLLEYGSGGTLVDVIDKLGGGGLVEADVRRYTRDLVRGIYWIHSKGYVHCDLKPDNVMMVGNCGTGEFRAKIGDLGLAKRGMRSNKKRKLDPYFRGTAMYLSPEAVADGIQECPCDIWALGCIVIEMFTGKPPWDGTEDLDANELLDRIGEGRESPKIPDEISVEAKNFLKGCLAVKPMYRLTAEMLLNHPFLEGLVDVIETEEIEEFSDVNADSSLVLLSEAEADDELNRFSCSEDDSFVTEEESVSYWCEEVTNGGQIVAFDEEGTLKIQESTHTASSRNDSEYDHALNTTVPTSTGLQYPVAFTIPTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5B6</accession>
    <name>A0A162A5B6_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_018852</name>
        <name evidence="3" type="ORF">DCAR_0521564</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM95610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A162A5B6"/>
    <dbReference type="Gramene" id="KZM95610">
        <property type="protein sequence ID" value="KZM95610"/>
        <property type="gene ID" value="DCAR_018852"/>
    </dbReference>
    <dbReference type="OMA" id="GHISWKT"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37721">
        <property type="entry name" value="OS05G0464200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37721:SF1">
        <property type="entry name" value="OS05G0464200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM95610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH02176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="70" mass="7331" checksum="8534C29B6D38950E" modified="2016-07-06" version="1">MELSSATPSNNKKKQFPPKRGQIKEDILKSFATSLSNLASKAGEVVSKIKQGSTSSAAASPPSSSYNSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162A5H2</accession>
    <name>A0A162A5H2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-quinone oxidoreductase subunit I</fullName>
            <ecNumber evidence="15">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NADH dehydrogenase I subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NDH-1 subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ndhI</name>
        <name evidence="15" type="synonym">nuoI</name>
        <name evidence="19" type="ORF">DCAR_0100081</name>
        <name evidence="18" type="ORF">DCAR_018912</name>
        <name evidence="20" type="ORF">DCAR_0C036330</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plastid" evidence="20"/>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="15">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 16">Belongs to the complex I 23 kDa subunit family.</text>
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    <keyword evidence="10 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0520">NAD</keyword>
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    <keyword evidence="4 15" id="KW-0874">Quinone</keyword>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="9 15" id="KW-1278">Translocase</keyword>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="17">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
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            <end position="124"/>
        </location>
    </feature>
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        <location>
            <position position="64"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
            <position position="67"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
            <position position="74"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
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        <location>
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        <ligand>
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        <location>
            <position position="107"/>
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        <ligand>
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        <ligand>
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        </ligand>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
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        <source>
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            <dbReference type="RuleBase" id="RU004412"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM95670.1"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG80936.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOH16769.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="164" mass="19269" checksum="422F455BE9160455" modified="2016-07-06" version="1">MFSMVTEFMNYSQQTVRAARYIGQGFMITLSHASRLPVTIQYPYEKLITSERFRGRIHFEFDKCIACEVCVRVCPIDLPVVDWKLEKDIRKKRLLNYSIDFGICIFCGNCVEYCPTNCLSMTEEYELSTYDRHELNYNQIALGRLPMSIINDYTTRTILNLPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162ACZ7</accession>
    <name>A0A162ACZ7_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="1" type="ORF">DCAR_013628</name>
        <name evidence="2" type="ORF">DCAR_0417390</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
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            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG98049.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="63" mass="7288" checksum="A564868FAE087434" modified="2016-07-06" version="1">MHNTTDLNSMLLISADSRWNLEYVSYNVALRTAIANTKRARRYVAPSAVEHRINTADRHIEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A162AYQ7</accession>
    <name>A0A162AYQ7_DAUCS</name>
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            <fullName evidence="10">Methyltransferase</fullName>
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    <gene>
        <name evidence="11" type="ORF">DCAR_0209706</name>
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    <organism evidence="11 12">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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            <taxon>asterids</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
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            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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                <person name="Spooner D."/>
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                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the methyltransferase superfamily.</text>
    </comment>
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        <location>
            <begin position="25"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="640" mass="72941" checksum="0D82594B32725ABC" modified="2016-07-06" version="1">MANSYSSTNILPHNLTLFKKKHINLYYTAIVTILCSMFYIIGVWQHGGGATGDTVLLSTLPCIPRENTTLRIDPLPELDFSSHHKAEDMQPAEARVKHFPPCDSKFSEYTPCEDAERSLKFDREMLVYRERHCPEDDEVLSCRIPAPFGYKQPFRWPESRDAVWYANVPHKHLTVEKAGQNWVRFEGDRFKFPGGGTMFPRGANAYIDDIGKLINLRDGSIRTAIDTGCGVASWGAYLLSRNILTVSFAPRDTHIAQVQFALERGVPALIGVLASIRLPYPSRAFDMAHCSRCLIPWGKNDGLYLIEVDRILRPGGYWIYSGPPINWQNRWKGWDRTPKDLEDEQNLVESVARSLCWKKLVQKDDIAIWQKPTNHVHCKLNRKVFKKPQLCQAPNPDKAWYTKLETCLTPLPEVKNIKETAGGQLAKWPSRLNAIPPRIASGSVEGVTNENFTKDSELWKTRILHYKSLDHQLAERGRYRNLLDMNSYLGGFAAALVDDPVWVMNIVPVEGQIDTLGVVYQRGLIGTYQNWCEAMSTYPRTYDFIHADSIFSLYKDRCGIEDIVLEMDRILRPQGSVIIRDDVDVLLNVKNILDGVEWESRLVDHEDGPLVRQKLLIATKQYWTSPAAIEQDPQALNRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164TBV8</accession>
    <name>A0A164TBV8_DAUCS</name>
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        <recommendedName>
            <fullName evidence="5">DUF1645 domain-containing protein</fullName>
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        <name evidence="2" type="ORF">DCAR_024458</name>
        <name evidence="3" type="ORF">DCAR_0727687</name>
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
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    </reference>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZM87324.1"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26415" checksum="4DBE2E6E80074729" modified="2016-07-06" version="1">MSYDNNAEMDSKQAQLRRKEVHEEDNKDEDFTFAISGGDSSPISADDAFEHGKIRPIYPLFDQNLLLNDGELSLPARPPVEHVFMESPARKSDEMERIATGPYCAWSKSPEMSRKSNSTGFSKLWRFKEYMQRSNSDGRDAYVFYNQNSTGEASSEKKIIAKKSASEKKAVVEEKKKAKKGDKTMSSHEVYLKKKGYNPEEKRKSYLPYRPELVGFFTNVNGGGLSKNVHPY</sequence>
</entry>
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>eudicotyledons</taxon>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
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                <person name="Iovene M."/>
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                <person name="Ashrafi H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Gramene" id="KZM87851">
        <property type="protein sequence ID" value="KZM87851"/>
        <property type="gene ID" value="DCAR_024952"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOH09189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="124" mass="13923" checksum="567CF7AB7E75A04E" modified="2016-07-06" version="1">MASAPVPSILQAPCINNCTNFIKLSPEPTETSRVIYRLGSFSETELKELKDKFISDLERVRSLLKQVESRELELVHAEDVDLREQGNSVDKASKIIGGKRTLPVSSDGEIKRKRLVKKTVDELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164W1E5</accession>
    <name>A0A164W1E5_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0624777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
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            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH05361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164W1E5"/>
    <dbReference type="Gramene" id="KZM91151">
        <property type="protein sequence ID" value="KZM91151"/>
        <property type="gene ID" value="DCAR_021484"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108225600"/>
    <dbReference type="OMA" id="SKWIISA"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF40">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE EPSILON 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="1">
        <location>
            <begin position="137"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOH05361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="284" mass="31724" checksum="240C85B6A865E5D4" modified="2016-07-06" version="1">MAASTAIVNGPAILPLSFFAKPLSCKLVSCTRLRAVNTRCRKLNAPDKLTFLSETIIKYKKRRIRNHITTYVSRDSSVEEGIGAHEQEAFDAELARFEMNQLEAFLNDLSKWLMVALFSLVLLLRHDAKAIWICAGSILNVGLSIALKRILNQERPSSATKSDPGMPSSHAQSLFFLSFVVILSMMDWLGMNIYTHLLAGLVLSLGSYFAWLRVSQGFHTLGQVTVGAVAGSVYVGLWFWIWDAIVLPAFISSLWIRLLVVVGGVGCILRFVVFLVYDWIMKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164X4T0</accession>
    <name>A0A164X4T0_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="1">eIF3j</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">DCAR_0626559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093348">
        <property type="protein sequence ID" value="WOH07130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164X4T0"/>
    <dbReference type="Gramene" id="KZM92705">
        <property type="protein sequence ID" value="KZM92705"/>
        <property type="gene ID" value="DCAR_019930"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224794"/>
    <dbReference type="OMA" id="ICISLEM"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH07130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="224" mass="25269" checksum="8BC36D01EA26C1C8" modified="2016-07-06" version="1">MDEWEDEPAPLVLKKEPVKSLWADEDLDEDDIKESWEDEDEPTPVPEAKPPVEKPPKKTVAKANDKKGKTAEVVKEEPLDPVAEKLRQQRLVEEADYRNTAELFGKKSGDDKSLDIFIPKSESDFLEYAELISHKLRPYEKSFHYIGLLKSVIRMSMTSLKASDAKEVASSVTAIVNEKLKAEKEANAGKKKTGAKKKQLIVDKPDDDAVVSNLDSSYDDFDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A164ZL90</accession>
    <name>A0A164ZL90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="2 10">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_019320</name>
        <name evidence="12" type="ORF">DCAR_0522109</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="2 10"/>
    <dbReference type="EMBL" id="LNRQ01000005">
        <property type="protein sequence ID" value="KZM96078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093347">
        <property type="protein sequence ID" value="WOH02720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A164ZL90"/>
    <dbReference type="STRING" id="79200.A0A164ZL90"/>
    <dbReference type="Gramene" id="KZM96078">
        <property type="protein sequence ID" value="KZM96078"/>
        <property type="gene ID" value="DCAR_019320"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224056"/>
    <dbReference type="OMA" id="YNEIGSH"/>
    <dbReference type="OrthoDB" id="1658at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000035">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4 10" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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</entry>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="10">Leaf</tissue>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Leaf</tissue>
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    <comment type="subcellular location">
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        </subcellularLocation>
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    <comment type="similarity">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG94526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="188" mass="20121" checksum="FA08CA1EA85A027A" modified="2016-07-06" version="1">MKCLGIIISLSVLLTIFIACSLDVDALSCSPSGKIRGRKAPQGQCNRENDSDCCVQGKLYPTYTCSPRVTGNTKATLTLNSFQKGGDGGGPSECDNQYHDDDTPVVALSTGWYSGGSRCLNKITISANGRSVEAMVVDECDSTMGCDDEHDYQPPCPNNIVDASKAVWKALGVPKDNWGDLDITWSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CA21</accession>
    <name>A0A166CA21_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">DCAR_012255</name>
        <name evidence="12" type="ORF">DCAR_0313881</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000003">
        <property type="protein sequence ID" value="KZN03499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093345">
        <property type="protein sequence ID" value="WOG94585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CA21"/>
    <dbReference type="Gramene" id="KZN03499">
        <property type="protein sequence ID" value="KZN03499"/>
        <property type="gene ID" value="DCAR_012255"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108215045"/>
    <dbReference type="OMA" id="PETTHMA"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009561">
        <property type="term" value="P:megagametogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055046">
        <property type="term" value="P:microgametogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000746">
        <property type="entry name" value="E3 ubiquitin-protein ligase RHF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463:SF78">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="325"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KZN03499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG94585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="381" mass="41178" checksum="FF62C77491368278" modified="2016-07-06" version="1">MEEDKKSENHLTSAAAFVEGGIQDACDDACSICLEVFSESEPSTVTACKHEFHLQCILEWCQRSSQCPMCWQAISLKDPNSQELLDAIERERSIRTTPSRNTAIFHHPTLGDFELQHLPVGATDAELEDRIIQHLAAAAAMGRARHFARREGQRNRSSAQGRPHFLVFSTNSNAPSATASSSPTEAGDGETNPSITVSGSSHIPLGINDEPVQPNTPQRPIQSVQASGSRVITENQHGTSPYRSPSRSSPNSQDRAGPSEFQSFSDTLKSRFNAMSSRYKESITKSTKGWKEKLFSRNSSATDTGGSEVRREVNVDVGTVSRMMGNLETNDNVPSPSISVSNTSEGRPVPEHGHQHTGGTNGATLNESNVQPSCAATSASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166CJI2</accession>
    <name>A0A166CJI2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pirin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_004836</name>
        <name evidence="9" type="ORF">DCAR_0205333</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000002">
        <property type="protein sequence ID" value="KZN03974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
        <property type="protein sequence ID" value="WOG86132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166CJI2"/>
    <dbReference type="Gramene" id="KZN03974">
        <property type="protein sequence ID" value="KZN03974"/>
        <property type="gene ID" value="DCAR_004836"/>
    </dbReference>
    <dbReference type="OMA" id="VAHRDNA"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000055">
        <property type="entry name" value="pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
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        </location>
    </feature>
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        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
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        <location>
            <position position="68"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
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        <location>
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        </location>
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        </ligand>
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        </ligand>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
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            <dbReference type="Pfam" id="PF02678"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KZN03974.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG86132.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <name evidence="4" type="ORF">DCAR_0207458</name>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN05784.1"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG88224.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
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                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
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                <person name="Bostan H."/>
                <person name="Rolling W."/>
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                <person name="Simon P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
            <begin position="61"/>
            <end position="75"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG89134.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
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    </evidence>
    <sequence length="193" mass="21455" checksum="1C8DD5F4321FA1FF" modified="2016-07-06" version="1">MANNEKQAASSPENLLDDFFGIKQHPDSASVLALLSSKPKDSDTANSAPTRRMPMCLGGSQAWNRNQGSFPQDSGANKENKLTFPQESVHPSPLSSSIYYGSLEDMYVQSSSSQASKSYPTYYKYENGYRREGRSVEDEGAATIKWWEGMVLNNGLTFLAYTRVSGIHYSATDVNCAEILNFEYMQDQSTIKK</sequence>
</entry>
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        <recommendedName>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
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                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
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                <person name="Grzebelus E."/>
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                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="1">Leaf</tissue>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000002">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093344">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="DCAR_007581"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KZN06744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG89324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20826" checksum="FF7D5DF11C6B2D8B" modified="2016-07-06" version="1">MEESGAMAAKRVWSMVRALYFMFRKDKSHKKIMLDIKMMLKRGKLARKAFQSLMFNQHDKMTIADSQQQLSVLPGEYIFSCSDTPLFRLHSRRNGSTSHRFFSCAHATPTLEDGDEMSVNVAVRKALEMLQSDHEVVRSPVGRIPDSLYRIGEVHEEESCVDEAAEEFIRKFYKDLKKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166F6J4</accession>
    <name>A0A166F6J4_DAUCS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">DCAR_0209316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_008238"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="783427at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35297">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35297:SF2">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="WOG90075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="150" mass="16902" checksum="FB8BBA90DB255277" modified="2016-07-06" version="1">MHRQSLGSPNSKLHTNGVLIAPNDIIELTADDLKTKHQLSSQLVAGDDDEELRKSQKPQKSPEKLIHIIPMLIIFCFLILYLTSHDPSQGVNNLKGFKRNSKPVEISSEIDDLRGVSAIEKSGVLAVRSLQEEEIYEMKRSRINRKLGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GDG0</accession>
    <name>A0A166GDG0_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0101440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GDG0"/>
    <dbReference type="Gramene" id="KZN08834">
        <property type="protein sequence ID" value="KZN08834"/>
        <property type="gene ID" value="DCAR_001490"/>
    </dbReference>
    <dbReference type="OMA" id="NINICNQ"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF36">
        <property type="entry name" value="BNAC01G27460D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG82277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="238" mass="27066" checksum="03BA4A5CB576516E" modified="2016-07-06" version="1">MQIILSAIGKLRGCVRQIENQNPSGASQIDILNRAKVLLEQDKIYDKGFKFDHVWEILKDSEKFGDDHSNATPYRQTQTSNFVSSQANSPATESPTSASPGLSSFSPDINDLSVDGSSSQRPIGVKKAKEKRKVEEHTSAIIDTIKEEQRQVIEILKKNSADRQQNYEIQMLRAQNEKRKLDMAPYLAENKILIKDLNSIDDPILREHFRNEQLKILQKRSTHDQAAPHGFHDDLPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166GMU3</accession>
    <name>A0A166GMU3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_001795</name>
        <name evidence="8" type="ORF">DCAR_0101795</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG82629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166GMU3"/>
    <dbReference type="STRING" id="79200.A0A166GMU3"/>
    <dbReference type="Gramene" id="KZN09139">
        <property type="protein sequence ID" value="KZN09139"/>
        <property type="gene ID" value="DCAR_001795"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204990"/>
    <dbReference type="OMA" id="EMMCEEC"/>
    <dbReference type="OrthoDB" id="778365at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
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    <dbReference type="InterPro" id="IPR043561">
        <property type="entry name" value="LHW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH155-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR EMB1444-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23176">
        <property type="entry name" value="bHLH_LHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="544"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KZN09139.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOG82629.1"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166IQC8</accession>
    <name>A0A166IQC8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
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        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166IQC8"/>
    <dbReference type="Gramene" id="KZN11374">
        <property type="protein sequence ID" value="KZN11374"/>
        <property type="gene ID" value="DCAR_004030"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108214928"/>
    <dbReference type="OMA" id="TIFKGRE"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF3">
        <property type="entry name" value="OS09G0540400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="365" mass="41115" checksum="616C7950F13FF044" modified="2016-07-06" version="1">MGGPCVNFLRFVIQFQLILLALAQKQTLRTNSGNLAIFAFGDSFVDPGNNNYIRTISKANFPPYGIDFENQIPTGRFTNGRLVTDYAASYAGIKDYVPPYLDRNLSMEELLTGVSFASAGSGYDPLTPTLTGGVISMPKQIDHFREYKSRIETQIGKERTEALIKNAVYVISAGSNDFLFNYFWVSGIRRLSYSLPRYYHLLMQQLQHFLQDLLDLGAGNIVMMGLPPLGCFPLVITLKSNPINGFLERQCVTRISDAAQGFNQIAKQKCKEMERVDSQIYYVSLYESYMDIFQDPKKFGFDKADTSCCGTGILEVSIFCNRRSRLCPNVSDHVFFDSLHPTERTYSLLFEALAPTIDLILQGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J3T8</accession>
    <name>A0A166J3T8_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">DCAR_0104779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J3T8"/>
    <dbReference type="Gramene" id="KZN11764">
        <property type="protein sequence ID" value="KZN11764"/>
        <property type="gene ID" value="DCAR_004420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="WOG85588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="105" mass="11764" checksum="78D969F8B7F1662A" modified="2016-07-06" version="1">MTLAPPMTSDHTDLKASESEDAFKSQHLESAAKLIIEFGSPLQLQQLIRVENRDQFDQYLCAVDEVQQSIKSGTASSYETHGIRAIKTLHTISRNIGMLNPCNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A166J9J2</accession>
    <name>A0A166J9J2_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="3 11">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">DCAR_0104972</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 11"/>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A166J9J2"/>
    <dbReference type="Gramene" id="KZN11932">
        <property type="protein sequence ID" value="KZN11932"/>
        <property type="gene ID" value="DCAR_004588"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108224438"/>
    <dbReference type="OMA" id="PGQVLGC"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="WOG85779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="514" mass="56127" checksum="728C733C98384188" modified="2016-07-06" version="1">MDTSEPTALALTNPNAAAAAGEPPRVRQQPKRFIKNQIPDSILNNASLNAAISLLPSNYSFEIHKSVWRVASTNAKRVALQFPEGLLMYSLVISDILTAFADVERVFILGDVTYGACCVDDLAAAALGADLLIHYGHSCLVPVDNTVIPCLYVFVDIKIDVSKLISTIQLNLADKISDVRNGSSDEVSDIRVNSGEKASDIGVNLDAKVSDIGDDLGDKVSDIRVNKIVLAGTIQFGTAIRVAKPELEKLGFRVLVPQSKPLSAGEVLGCTAPTIVGKEVGEVGENVLVFVADGRFHLEAFMIANPGVKAFRYDPYMGKLFLEEYDHEGMKECRRNAILKAREAKNWGIVLGTLGRQGNPRILDRLEKKMKEKDLDWTVVLMSELSPARIALFGDSVDAWIQIACPRLSIDWGDAFVKPLLNPFEAEIALGDLPGWWEKSGMNSDCNTGVGCEKNESCCRGDCTGKKEEGKTVADYPMDYYAQDGGEWNSSYSKKQTRPSRRILVSGKENGHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A169WT90</accession>
    <name>A0A169WT90_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">DCAR_002513</name>
        <name evidence="20" type="ORF">DCAR_0102619</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LNRQ01000001">
        <property type="protein sequence ID" value="KZN09857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG83444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A169WT90"/>
    <dbReference type="Gramene" id="KZN09857">
        <property type="protein sequence ID" value="KZN09857"/>
        <property type="gene ID" value="DCAR_002513"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108204654"/>
    <dbReference type="OMA" id="PHWASMI"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000329">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF47">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ROCO5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0157">Chromophore</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="18">
        <location>
            <begin position="100"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="482"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="272"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KZN09857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="WOG83444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="761" mass="84963" checksum="90B8E1B2CA40DAC0" modified="2016-07-06" version="1">MENKTEELIKKIQVLESEHEQLKQEMSKLIISGDHHRRSQSISPQRRRRFAGDSGSLAAFRKGSASFRHSSPLRRESRSTVVAAGAEPENTAAAAAAEFSDSQYLSILESMGQSVHIFDLNFHIFYWNPTAQRLYGYSATEAHGQNVIDLLLDPEDCVSGHNILNHTLSMRETWTGKFPTKTKRGARLEVIATNSPFYDDNGNLIGVICVSNDSRPFEDMSSALLAAQKVDTDSSVVSRPRRIASAKLGLNPQQPLQVAIASKISDLASKVSSKVKSKMKTDDGIMDREGGSGGSHNSDHGFSDTSLFEQKEDAASSGGSTPRGEIHPSSFGVFSHTAHDVYLNGKESRDCGDEYEGKIGIHKIITSKAEAWMSKKNITWPWKVNGQERLEPRSANTVLPQLNAAQESNLAHKMTHNTSARPDNHEFENNRSPHKEAPGSWPSINVYSSGSLSSCGSTSSSTINRVDMEADSLDCEILWEDLTIGEQIGQGSCGTVYHGLWYGSDVAIKVFSKQEYSDDVIFAFREEVALMKRLRHPNILLFMGAVSSPERLCIVTEFLPRGSLFRLLQKGTSQKSASRLDWRRRVHMALDIARGMNYLHHCSPPIVHRDLKSSNLLVDKSWNVKVGDFGLSRLKYGQFLETITGKGTPQWMAPEVLRNEPSDEKSDVYSYGVVLWELATEKIPWDHLNSMQVIGAVGFMNQRLEIPNDVDARWASLIESCFQSEPKDRPTFQELLERLKDLQRQYAIQFKQNTSMQDIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YB34</accession>
    <name>A0A175YB34_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">DCAR_0101005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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    </evidence>
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</entry>
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    </protein>
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            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
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            <taxon>Apiaceae</taxon>
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            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
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        </citation>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
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                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
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    </reference>
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        <text evidence="5">May play a role in photosystem I and II biogenesis.</text>
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        <text evidence="5">Originally thought to be a component of PSII; based on experiments in Synechocystis, N.tabacum and barley, and its absence from PSII in T.elongatus and T.vulcanus, this is probably not true.</text>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="WOH16743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="43" mass="4722" checksum="060A87AA741707AD" modified="2016-09-07" version="1">METATLVAIFISGLLVSFTGYALYTAFGQPSQQLRDPFEEHGD</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="8">Cation/H+ exchanger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">DCAR_027943</name>
        <name evidence="6" type="ORF">DCAR_0831503</name>
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        <name type="scientific">Daucus carota subsp. sativus</name>
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        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
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                <person name="Huang J."/>
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                <person name="Sanseverino W."/>
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                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <tissue evidence="5">Leaf</tissue>
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        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM84635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="DCAR_027943"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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        <recommendedName>
            <fullName evidence="1">DUF3700 domain-containing protein</fullName>
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    </protein>
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        <name evidence="3" type="ORF">DCAR_0832389</name>
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    <organism evidence="2">
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            <taxon>Apiaceae</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
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                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH12880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YPM7"/>
    <dbReference type="Gramene" id="KZM85413">
        <property type="protein sequence ID" value="KZM85413"/>
        <property type="gene ID" value="DCAR_027165"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199779"/>
    <dbReference type="OMA" id="KDEEMWG"/>
    <dbReference type="OrthoDB" id="2019121at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd01910">
        <property type="entry name" value="Wali7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024286">
        <property type="entry name" value="DUF3700"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044828">
        <property type="entry name" value="TSJT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952">
        <property type="entry name" value="ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952:SF5">
        <property type="entry name" value="ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12481">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01172">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3700" evidence="1">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KZM85413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="WOH12880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="237" mass="25433" checksum="2968FF1A38CFC0EA" modified="2016-09-07" version="1">MLAVFSSSIVSPPDELVAAGSRTPSPKITSTALLKRFLGSDASAVSMQIGDDAQLAYTHNNQSALRPRSFAVKDEIFCLFEGALDNLGSLKQQYGLGKSANEVVLVIEAYKTLRDRAPYPPNHVVSHLDGNFAFIVFDKSTSTLFVAADQSGKVPLYWGITADGYVAIANDSDLLKGACGKSLASFPQGCFFSTDNGELRSYENPKNKITAVPATEEEIWGAKYMVEGVPVLAAINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YQ16</accession>
    <name>A0A175YQ16_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">DCAR_026863</name>
        <name evidence="8" type="ORF">DCAR_0832718</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="LNRQ01000008">
        <property type="protein sequence ID" value="KZM85715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YQ16"/>
    <dbReference type="STRING" id="79200.A0A175YQ16"/>
    <dbReference type="Gramene" id="KZM85715">
        <property type="protein sequence ID" value="KZM85715"/>
        <property type="gene ID" value="DCAR_026863"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108199221"/>
    <dbReference type="OMA" id="MIRCIAN"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000163">
        <property type="entry name" value="Geraniol 8-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF14">
        <property type="entry name" value="CYTOCHROME P450 76A2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KZM85715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="WOH13209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="48730" checksum="E7DC32E0A5C97E73" modified="2016-09-07" version="1">MVILSAGAAEELFKNHDLSFVDRFTNDSMRSHDHYTVSIAFGSYNAYWRTLRRICASELFANKRINNTVLIRQKCVDEMLLWIENEVKKSTSSSIVVRDFVFPALFNMIGNLTLSHNLVDPQSEMSSEFCTALAGFHESVGRPNISDLLPWLRRLDLQGIRKASDYSLAKAKEIISAFVKERVIEREIKHEPSSEQKDFLDVLLDYRGTGKDEPAELSESQVTVFLMEMFFAGTDTSSATIEWAMSELLTNPDRLKKIRAELASVVGANRKLQETDIENLPYLRAIVEETLRLHPPAPLLIPRKAVQDTNFMGYSILRNTQVMINCWAIGRDEESWEDALCFKPERFLDSDTNYKGQSFEFIPFGAGRRMCPAVPLAHRMVHLILGSLLHHFEWELCEDGGVIDMRETMGVTAKKLEALQAIPKRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A175YRS9</accession>
    <name>A0A175YRS9_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0832854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093350">
        <property type="protein sequence ID" value="WOH13344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A175YRS9"/>
    <dbReference type="Gramene" id="KZM85828">
        <property type="protein sequence ID" value="KZM85828"/>
        <property type="gene ID" value="DCAR_026750"/>
    </dbReference>
    <dbReference type="KEGG" id="dcr:108198542"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF75">
        <property type="entry name" value="RIBONUCLEASE H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="135"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="958"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="WOG84202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1642" mass="187594" checksum="BDB19BF7004D3324" modified="2024-05-29" version="1">MESGKRSDFQISNSQTTFRRSSGGDKSKQDVGDKLVNNFIWAHNHLVEKRVLERLNFGDRSAIQDALDQIHARSLDDNPFKGVRSYSEVLKNSEKGHATGFSVGDQPDPKEEEWQEVSYKRRQSARPKQGVNYRVTIFLHNIPENATGSQIWRLFKSCGHVLDIILPKKRDVRGSRYGFVHTISELEAGAIINNAKMDRALGGRISMSINGISNQQGRGQGKANWENKTNPKDFGKKMFEFTELEIDEEVETALLECKIGFSWFEESATIMQERLNDIGLENYRITSLSKRKESDHIISKVTWIECKGLPMPAWKDENLKAITSRFGEWISWTYQSDSLNEFFNPLICIDTVLPDNICDRMKVLPMEFSNNQEHVGSVNTQKDVTGLSHSERISSGSVTNGPLPIIEMPMSKEQDSKEKIIEGGGLIKNLRVITPTSQRHSSEFRSYVSSPEESSNTSHQHDSLPGSEICNSELNSSIQTDLCIDVSKKLRVKSNRGRPKKVNNGVRNPFEIGIKFKGRKRKAGGGRKVNRSRRVKEAPNCLQIVSTKLTGRSTMEALEIIRSAELMGLEIKGDRELAVKEIARKLDNGEFHVVSVYSPLDLSLKMSLWEDIRNILSLIDKEPLAVVGDFNCIRRRSEQGNCVYSRKDMDLFDGWIEDCNLLELPLLNDMYTWFGPVGKKSKLDRILVNHEWFQTGNWNVKSLSRKHSDHSPLFTFMVGMSRVSKPFKIFNCFLTEELFEGIQKEMWRDDQWRSQNIHVVLKRIKTKIKEMTRGSRDKINTEILALENKLRIADNSTVDQGWKTQGEIREQLLQLYSKRESMLKQKARLSWLKLGDGNTSFFHRTVQIRRHRNNIKRIIWNDVWISDPEGIKRTFFQFFSSFYKLEVTSPLGLGMLTLPWLEDHAKSSLIKPILVQEVEAVLHNLEDDKAPGPDGLNIRSLKFLWPIIKSKIMCFLEHFQSTATLPTGINASFIALIPKIAEPTLVNHYRPISLINSAVKLLTKILASRLACHMNAIVSDSQTGFIKGRQASESILLVKEIVHSIQKGKGRGMVLKLDFEKAFDTVKWEFLFEVLAKMNFDQKWIGWIKGLLETARVSILVNGSPTKEFVPSRGLRQGDPLSPLLFNLVGEVLSSMLHKTSQKGIFKGFSLGKAMQPITHLQFADDTIVFVDNTLESISGVKKVLQCFQMISGLKINYEKSEIFAHNSSFSTQMEGARIFGCKVGQWPMTYLGVPIGISSKRRIFWEPLVKKFHSKLATWKADILNLAGRTTLVKSVLDSLPTYWLGLHMIPPGVCDKIERIRRDFLWGSVGGKEEPKRKIHAIRWSRICLSKDQGGLGLVPIKVKNLALLGKWSFRWDNERHRNWNIWIRGKYNCSKHSNLQESLFCSWWNFEDDFLEGLNPCNLWDSAKLIRYKKAKFAWQVVISATLWVIWLSRNDAIFNNVPVHVRHNLIFLKSLAREWCLAENLITVSSTTWWDHNPMGSITKSEDDQMKALSQVNWDLIAFLDGSWKILQGSNQAGIGGYIQQKNGNAIFSFSGPSSACSAFQAEWNALTFLLTNFAKSRWSDHSLLIYSDCKSLICKFLELFTSDVEEEYIYLARLIKPMKVYIKLISRDLNCRADMLAKQGAHNSQMAHFWAHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W7B3</accession>
    <name>A0AAF0W7B3_DAUCS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">DCAR_0103761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG84577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAF0W7B3"/>
    <dbReference type="KEGG" id="dcr:108197017"/>
    <dbReference type="Proteomes" id="UP000077755">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042296518" description="AMP-dependent synthetase/ligase domain-containing protein" evidence="8">
        <location>
            <begin position="16"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="100"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="WOG84577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000077755"/>
        </source>
    </evidence>
    <sequence length="694" mass="76162" checksum="E1AD2736FD0DC44F" modified="2024-05-29" version="1">MGPYLVGVVVPIVLALLLHSSKKGKKRGLPVDVGGQPGFAIRNSRFTSPSESSQAGISTLAELFEQSCKKHHDKKFLGTRRLISSEIEVSEDGRSLEKLHLGDYEWLSYCKAFETVCNFASGLVQLGHNKERLAIFADTQEKWMIALQGCFRNNVTVVTIYASLGEEALRHSLNETEVTTVVCGKRELDKLLDVSGQLTTVKRVICMDDEISAHKSTIQSYWTITSFSNVERLGRDKPVTANLPVSTDIAVIMYTSGSTGLPKGVMVTHGNILSAVYGFMKIVPDIGSKDTHLAYLPLAHILELAAENIVVAAGCSIGYGSPLTLTDTSNKIKSGTKGDVSALRPTLMPAVPAILDRIRDGVWKKVDATGGLPKKLFDLAYARRLAAINGSWFGAWGLESLFWNFLIFRKIRKILGGRVRFMLSGGAPLSGNTQRFTNICLGVPVVQGYGLTETCAAATFSEHDDTSIGRVGPPLSCAIIKLIDWPEGGYLANDSPMPRGEIVVGGPHVSVGYFKNDEKTKEVYKVDDRGMRWFYTGDIGRFHDDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALVISPYVEIIVLHADPYRDHCVALVVVCRPALEDWALSQGIKYVSFSDLCFKEDAVKEVLGSLRKAGKEAKLEKFEIPTKIKLLSEMWNLESGLVTAALKIKRDAIRKTFAAELAQLYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAF0W868</accession>
    <name>A0AAF0W868_DAUCS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">DCAR_0104228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Daucus carota subsp. sativus</name>
        <name type="common">Carrot</name>
        <dbReference type="NCBI Taxonomy" id="79200"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Apiales</taxon>
            <taxon>Apiaceae</taxon>
            <taxon>Apioideae</taxon>
            <taxon>Scandiceae</taxon>
            <taxon>Daucinae</taxon>
            <taxon>Daucus</taxon>
            <taxon>Daucus sect. Daucus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Genet." volume="48" first="657" last="666">
            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Zeng P."/>
                <person name="Satapoomin P."/>
                <person name="Huang J."/>
                <person name="Bowman M."/>
                <person name="Iovene M."/>
                <person name="Sanseverino W."/>
                <person name="Cavagnaro P."/>
                <person name="Yildiz M."/>
                <person name="Macko-Podgorni A."/>
                <person name="Moranska E."/>
                <person name="Grzebelus E."/>
                <person name="Grzebelus D."/>
                <person name="Ashrafi H."/>
                <person name="Zheng Z."/>
                <person name="Cheng S."/>
                <person name="Spooner D."/>
                <person name="Van Deynze A."/>
                <person name="Simon P."/>
            </authorList>
            <dbReference type="PubMed" id="27158781"/>
            <dbReference type="DOI" id="10.1038/ng.3565"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2022-03" db="EMBL/GenBank/DDBJ databases">
            <title>Draft title - Genomic analysis of global carrot germplasm unveils the trajectory of domestication and the origin of high carotenoid orange carrot.</title>
            <authorList>
                <person name="Iorizzo M."/>
                <person name="Ellison S."/>
                <person name="Senalik D."/>
                <person name="Macko-Podgorni A."/>
                <person name="Grzebelus D."/>
                <person name="Bostan H."/>
                <person name="Rolling W."/>
                <person name="Curaba J."/>
                <person name="Simon P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP093343">
        <property type="protein sequence ID" value="WOG85042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <title>A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.</title>
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                <person name="Senalik D."/>
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[file cut: first 33554432 bytes kept]
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